Rh3CG145500

F-actin-capping proteins bind in a Ca(2 )-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0008545
Physical Location & Seq
Reverse (-)
12085010 .. 12086893
1884 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG145500.1

Sequence Viewer

Length: 480 bp
ATGAAAGGCTATCATTTTCCTCAAGGGTGTGCTTTGGATGGAGAAATACTTAGATATGTGGGTGAAGCTTATCCAAAAGGGGTCTGCTCAGTGTGTTGTGGGAATGGTAAAGATGCAGAGGGCCCAGGATCTGACTTTGAGCTCGTGGTAGTGATTTCAGCTGCTAGACATAGCCCACAAAATTTCTGCAATGGTAGTTGGCGTTCAGTGTGGAGCATTGAGTTCAAAGATGAAGTACAAATGCTGGAATTAAAAGGCAAACTGCAGGTGGGTGCCCACTATTTTGAGGAGGGGAATGTGCAGTTAGATGCAAAACATGAATGCAAAGATTCAACAATTTTTCAGTCCTCTGAAGATTCTGCAATTACCATAGGCAACATTATAAGGCAACATAAGGCAGAATACTTGGCATCTCTTGAGGCATCCTATTCAAACTTGCCCGATACCACTTTTAAGGTACCATTCAAAATTCCGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
F-actin_cap_A

Protein Analysis

159

Amino Acids

17.57

Weight (kDa)

5.2

Isoelectric Point (pI)

36.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-actin_cap_A PF01267 11 - 152 2.2e-35 F-actin capping protein alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 383
AarI CACCTGC 1 cut(s) 256
Acc36I ACCTGC 1 cut(s) 256
Acc65I GGTACC 1 cut(s) 457
AccB1I GGYRCC 2 cut(s) 272, 457
AccIII TCCGGA 1 cut(s) 472
AclWI GGATC 1 cut(s) 136
AcsI RAATTY 2 cut(s) 181, 468
AcuI CTGAAG 1 cut(s) 372
AfaI GTAC 2 cut(s) 237, 459
AgsI TTSAA 4 cut(s) 226, 333, 432, 466
AjnI CCWGG 1 cut(s) 124
AluBI AGCT 3 cut(s) 68, 142, 161
AluI AGCT 3 cut(s) 68, 142, 161
Alw21I GWGCWC 1 cut(s) 144
AlwI GGATC 1 cut(s) 136
AlwNI CAGNNNCTG 1 cut(s) 131
Aor13HI TCCGGA 1 cut(s) 472
AoxI GGCC 1 cut(s) 121
ApaI GGGCCC 1 cut(s) 125
ApeKI GCWGC 1 cut(s) 161
ApoI RAATTY 2 cut(s) 181, 468
Asp718I GGTACC 1 cut(s) 457
AspS9I GGNCC 2 cut(s) 121, 122
AsuHPI GGTGA 1 cut(s) 74
BaeGI GKGCMC 2 cut(s) 125, 277
BanI GGYRCC 2 cut(s) 272, 457
BanII GRGCYC 2 cut(s) 125, 144
BauI CACGAG 1 cut(s) 143
Bbv12I GWGCWC 1 cut(s) 144
BbvI GCAGC 1 cut(s) 148
BccI CCATC 1 cut(s) 32
BciT130I CCWGG 1 cut(s) 126
BfaI CTAG 1 cut(s) 165
BfmI CTRYAG 1 cut(s) 263
BfuAI ACCTGC 1 cut(s) 256
BisI GCNGC 1 cut(s) 162
BlsI GCNGC 1 cut(s) 163
Bme1390I CCNGG 1 cut(s) 126
BmgT120I GGNCC 2 cut(s) 121, 122
BmiI GGNNCC 3 cut(s) 123, 274, 459
BmrFI CCNGG 1 cut(s) 126
BmsI GCATC 4 cut(s) 103, 298, 419, 431
BpuEI CTTGAG 2 cut(s) 6, 437
BsaJI CCNNGG 1 cut(s) 124
BsaWI WCCGGW 1 cut(s) 472
Bse3DI GCAATG 1 cut(s) 196
BseAI TCCGGA 1 cut(s) 472
BseBI CCWGG 1 cut(s) 126
BseDI CCNNGG 1 cut(s) 124
BseGI GGATG 2 cut(s) 43, 422
BseMI GCAATG 1 cut(s) 196
BseMII CTCAG 1 cut(s) 102
BseRI GAGGAG 1 cut(s) 302
BseSI GKGCMC 2 cut(s) 125, 277
BseXI GCAGC 1 cut(s) 148
BsgI GTGCAG 1 cut(s) 320
BshFI GGCC 1 cut(s) 123
BshNI GGYRCC 2 cut(s) 272, 457
BsiHKAI GWGCWC 1 cut(s) 144
BsiSI CCGG 1 cut(s) 473
BsmI GAATGC 1 cut(s) 326
BsnI GGCC 1 cut(s) 123
Bsp120I GGGCCC 1 cut(s) 121
Bsp1286I GDGCHC 3 cut(s) 125, 144, 277
Bsp13I TCCGGA 1 cut(s) 472
Bsp143I GATC 1 cut(s) 128
BspANI GGCC 1 cut(s) 123
BspCNI CTCAG 1 cut(s) 101
BspEI TCCGGA 1 cut(s) 472
BspLI GGNNCC 3 cut(s) 123, 274, 459
BspMAI CTGCAG 1 cut(s) 267
BspMI ACCTGC 1 cut(s) 256
BspPI GGATC 1 cut(s) 136
BspT107I GGYRCC 2 cut(s) 272, 457
BsrDI GCAATG 1 cut(s) 196
BssECI CCNNGG 1 cut(s) 124
BssMI GATC 1 cut(s) 128
BssSI CACGAG 1 cut(s) 143
Bst2BI CACGAG 1 cut(s) 143
Bst2UI CCWGG 1 cut(s) 126
BstDEI CTNAG 2 cut(s) 50, 88
BstF5I GGATG 2 cut(s) 43, 422
BstKTI GATC 1 cut(s) 131
BstMBI GATC 1 cut(s) 128
BstNI CCWGG 1 cut(s) 126
BstSCI CCNGG 1 cut(s) 124
BstSFI CTRYAG 1 cut(s) 263
BstSLI GKGCMC 2 cut(s) 125, 277
BstV1I GCAGC 1 cut(s) 148
BstX2I RGATCY 1 cut(s) 128
BstYI RGATCY 1 cut(s) 128
BsuRI GGCC 1 cut(s) 123
BtsCI GGATG 2 cut(s) 43, 422
BtsIMutI CAGTG 2 cut(s) 96, 213
BveI ACCTGC 1 cut(s) 256
CaiI CAGNNNCTG 1 cut(s) 131
Cfr13I GGNCC 2 cut(s) 121, 122
Csp6I GTAC 2 cut(s) 236, 458
CviAII CATG 1 cut(s) 317
CviJI RGCY 6 cut(s) 9, 68, 123, 142, 161, 174
CviKI_1 RGCY 6 cut(s) 9, 68, 123, 142, 161, 174
CviQI GTAC 2 cut(s) 236, 458
DdeI CTNAG 2 cut(s) 50, 88
DpnI GATC 1 cut(s) 130
DpnII GATC 1 cut(s) 128
Ecl136II GAGCTC 1 cut(s) 142
Eco24I GRGCYC 2 cut(s) 125, 144
Eco53kI GAGCTC 1 cut(s) 142
Eco57I CTGAAG 1 cut(s) 372
EcoICRI GAGCTC 1 cut(s) 142
EcoO109I RGGNCCY 1 cut(s) 121
EcoRII CCWGG 1 cut(s) 124
EcoT38I GRGCYC 2 cut(s) 125, 144
FaeI CATG 1 cut(s) 320
FaiI YATR 6 cut(s) 57, 171, 318, 371, 383, 393
FatI CATG 1 cut(s) 316
Fnu4HI GCNGC 1 cut(s) 162
FokI GGATG 2 cut(s) 50, 409
FriOI GRGCYC 2 cut(s) 125, 144
Fsp4HI GCNGC 1 cut(s) 162
FspBI CTAG 1 cut(s) 165
GluI GCNGC 1 cut(s) 162
HaeIII GGCC 1 cut(s) 123
HapII CCGG 1 cut(s) 473
Hin1II CATG 1 cut(s) 320
HindIII AAGCTT 1 cut(s) 66
HinfI GANTC 2 cut(s) 329, 356
HpaII CCGG 1 cut(s) 473
HphI GGTGA 1 cut(s) 74
Hpy188I TCNGA 2 cut(s) 133, 352
Hpy188III TCNNGA 2 cut(s) 416, 473
HpyCH4V TGCA 7 cut(s) 116, 189, 265, 301, 311, 324, 362
HpyF3I CTNAG 2 cut(s) 50, 88
Hsp92II CATG 1 cut(s) 320
Kpn2I TCCGGA 1 cut(s) 472
KpnI GGTACC 1 cut(s) 461
Kzo9I GATC 1 cut(s) 128
LmnI GCTCC 1 cut(s) 213
LpnPI CCDG 4 cut(s) 111, 138, 230, 251
Lsp1109I GCAGC 1 cut(s) 148
LweI GCATC 4 cut(s) 103, 298, 419, 431
MaeI CTAG 1 cut(s) 165
MalI GATC 1 cut(s) 130
MboI GATC 1 cut(s) 128
MboII GAAGA 1 cut(s) 365
MflI RGATCY 1 cut(s) 128
MhlI GDGCHC 3 cut(s) 125, 144, 277
MluCI AATT 5 cut(s) 181, 248, 336, 363, 468
MnlI CCTC 6 cut(s) 30, 112, 280, 283, 358, 412
MroI TCCGGA 1 cut(s) 472
MseI TTAA 2 cut(s) 251, 453
MspA1I CMGCKG 1 cut(s) 161
MspI CCGG 1 cut(s) 473
MspR9I CCNGG 1 cut(s) 126
Mva1269I GAATGC 1 cut(s) 326
MvaI CCWGG 1 cut(s) 126
NdeII GATC 1 cut(s) 128
NlaIII CATG 1 cut(s) 320
NlaIV GGNNCC 3 cut(s) 123, 274, 459
PaqCI CACCTGC 1 cut(s) 256
PctI GAATGC 1 cut(s) 326
PfeI GAWTC 2 cut(s) 329, 356
PkrI GCNGC 1 cut(s) 163
PsiI TTATAA 1 cut(s) 383
Psp124BI GAGCTC 1 cut(s) 144
Psp6I CCWGG 1 cut(s) 124
PspGI CCWGG 1 cut(s) 124
PspN4I GGNNCC 3 cut(s) 123, 274, 459
PspOMI GGGCCC 1 cut(s) 121
PspPI GGNCC 2 cut(s) 121, 122
PsrI GAACNNNNNNTAC 2 cut(s) 187, 219
PstI CTGCAG 1 cut(s) 267
PstNI CAGNNNCTG 1 cut(s) 131
PsuI RGATCY 1 cut(s) 128
PvuII CAGCTG 1 cut(s) 161
RsaI GTAC 2 cut(s) 237, 459
RsaNI GTAC 2 cut(s) 236, 458
SacI GAGCTC 1 cut(s) 144
SaqAI TTAA 2 cut(s) 251, 453
SatI GCNGC 1 cut(s) 162
Sau3AI GATC 1 cut(s) 128
Sau96I GGNCC 2 cut(s) 121, 122
ScrFI CCNGG 1 cut(s) 126
SduI GDGCHC 3 cut(s) 125, 144, 277
SetI ASST 5 cut(s) 70, 144, 163, 270, 459
SfaNI GCATC 4 cut(s) 103, 298, 419, 431
SfcI CTRYAG 1 cut(s) 263
SmlI CTYRAG 2 cut(s) 21, 416
SmoI CTYRAG 2 cut(s) 21, 416
Sse9I AATT 5 cut(s) 181, 248, 336, 363, 468
SspMI CTAG 1 cut(s) 165
SstI GAGCTC 1 cut(s) 144
StyD4I CCNGG 1 cut(s) 124
TasI AATT 5 cut(s) 181, 248, 336, 363, 468
TatI WGTACW 1 cut(s) 235
TfiI GAWTC 2 cut(s) 329, 356
Tru1I TTAA 2 cut(s) 251, 453
Tru9I TTAA 2 cut(s) 251, 453
TscAI CASTG 2 cut(s) 96, 213
TseI GCWGC 1 cut(s) 161
TspDTI ATGAA 3 cut(s) 17, 246, 333
TspRI CASTG 2 cut(s) 96, 213
XapI RAATTY 2 cut(s) 181, 468
XspI CTAG 1 cut(s) 165
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.