Rmu_sc0033180.1_g000001

F-actin-capping proteins bind in a Ca(2 )-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0033180.1
OG0008545
Physical Location & Seq
Reverse (-)
2 .. 3046
3045 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0033180.1_g000001.1.cds

Sequence Viewer

Length: 674 bp
atgcctggtggaattggagacgttctagttacatctttcggagagctccgtgggaatgagtaccttgatcccaggactgctcatgttgctgttgttgaccatatcaaacaggtttgtccagacgtgagacctgcattggatgaggaacttccttccgcatatgttgaggaatacaggtgtgctttggatggagaaatacttagatatgtgggtgaagcttatccaaaaggagtctgctcagtgtactgtgggaatggtaaagatgcagagggccctggatctgactttgagctcgtggtagtgatttcagctgctagacatagcccacaaaatttctgcaatggtagttggcgttcagtgtggagtattgagttcaaagatgaagtacaagtgctggaattaaaaggcaaactgcaggtgggtgcccactattttgaggaggggaatgtgcagttagatgcaaaacatgaatgcaaagattcaacaatttttcagtcctctgaagattctgcaattaccataggcaacattataaggcaacatgaggcagaatacttggcatctcttgaggcatcctattcaaacttgcccgataccacttttaaggatcttcgtagaaagcttccagttactcgcaccctatttccatggcagaatactttacagtttagctt
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
F-actin_cap_A

Protein Analysis

224

Amino Acids

24.96

Weight (kDa)

4.87

Isoelectric Point (pI)

40.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 533
AarI CACCTGC 1 cut(s) 406
Acc36I ACCTGC 2 cut(s) 139, 406
AccB1I GGYRCC 1 cut(s) 422
AciI CCGC 1 cut(s) 156
AclWI GGATC 3 cut(s) 62, 286, 615
AcsI RAATTY 1 cut(s) 331
AcuI CTGAAG 1 cut(s) 522
AfaI GTAC 3 cut(s) 62, 245, 387
AgsI TTSAA 3 cut(s) 376, 483, 582
AjiI CACGTC 1 cut(s) 124
AjnI CCWGG 3 cut(s) 4, 71, 274
AluBI AGCT 6 cut(s) 46, 218, 292, 311, 622, 672
AluI AGCT 6 cut(s) 46, 218, 292, 311, 622, 672
Alw21I GWGCWC 2 cut(s) 48, 294
Alw26I GTCTC 2 cut(s) 12, 121
AlwI GGATC 3 cut(s) 62, 286, 615
AoxI GGCC 1 cut(s) 271
ApaI GGGCCC 1 cut(s) 275
ApeKI GCWGC 1 cut(s) 311
ApoI RAATTY 1 cut(s) 331
AspS9I GGNCC 2 cut(s) 271, 272
AsuHPI GGTGA 1 cut(s) 224
BaeGI GKGCMC 2 cut(s) 275, 427
BanI GGYRCC 1 cut(s) 422
BanII GRGCYC 3 cut(s) 48, 275, 294
BauI CACGAG 1 cut(s) 293
Bbv12I GWGCWC 2 cut(s) 48, 294
BbvI GCAGC 1 cut(s) 298
BccI CCATC 1 cut(s) 182
BciT130I CCWGG 3 cut(s) 6, 73, 276
BcoDI GTCTC 2 cut(s) 12, 121
BfaI CTAG 2 cut(s) 26, 315
BfmI CTRYAG 1 cut(s) 413
BfuAI ACCTGC 2 cut(s) 139, 406
BisI GCNGC 1 cut(s) 312
BlsI GCNGC 1 cut(s) 313
Bme1390I CCNGG 3 cut(s) 6, 73, 276
BmgBI CACGTC 1 cut(s) 124
BmgT120I GGNCC 2 cut(s) 271, 272
BmiI GGNNCC 2 cut(s) 273, 424
BmrFI CCNGG 3 cut(s) 6, 73, 276
BmsI GCATC 4 cut(s) 253, 448, 569, 581
BpuEI CTTGAG 1 cut(s) 587
BsaI GGTCTC 1 cut(s) 121
BsaJI CCNNGG 4 cut(s) 49, 71, 274, 647
Bse1I ACTGG 1 cut(s) 626
Bse3DI GCAATG 1 cut(s) 346
BseBI CCWGG 3 cut(s) 6, 73, 276
BseDI CCNNGG 4 cut(s) 49, 71, 274, 647
BseGI GGATG 3 cut(s) 145, 193, 572
BseMI GCAATG 1 cut(s) 346
BseMII CTCAG 1 cut(s) 252
BseNI ACTGG 1 cut(s) 626
BseRI GAGGAG 1 cut(s) 452
BseSI GKGCMC 2 cut(s) 275, 427
BseXI GCAGC 1 cut(s) 298
BsgI GTGCAG 1 cut(s) 470
BshFI GGCC 1 cut(s) 273
BshNI GGYRCC 1 cut(s) 422
BsiHKAI GWGCWC 2 cut(s) 48, 294
BsmAI GTCTC 2 cut(s) 12, 121
BsmBI CGTCTC 1 cut(s) 12
BsmI GAATGC 1 cut(s) 476
BsnI GGCC 1 cut(s) 273
Bso31I GGTCTC 1 cut(s) 121
Bsp120I GGGCCC 1 cut(s) 271
Bsp1286I GDGCHC 4 cut(s) 48, 275, 294, 427
Bsp143I GATC 3 cut(s) 67, 278, 607
Bsp19I CCATGG 1 cut(s) 647
BspACI CCGC 1 cut(s) 156
BspANI GGCC 1 cut(s) 273
BspCNI CTCAG 1 cut(s) 251
BspLI GGNNCC 2 cut(s) 273, 424
BspMAI CTGCAG 1 cut(s) 417
BspMI ACCTGC 2 cut(s) 139, 406
BspPI GGATC 3 cut(s) 62, 286, 615
BspT107I GGYRCC 1 cut(s) 422
BspTNI GGTCTC 1 cut(s) 121
BsrDI GCAATG 1 cut(s) 346
BsrI ACTGG 1 cut(s) 626
BssECI CCNNGG 4 cut(s) 49, 71, 274, 647
BssMI GATC 3 cut(s) 67, 278, 607
BssSI CACGAG 1 cut(s) 293
BssT1I CCWWGG 1 cut(s) 647
Bst2BI CACGAG 1 cut(s) 293
Bst2UI CCWGG 3 cut(s) 6, 73, 276
Bst4CI ACNGT 2 cut(s) 248, 666
BstDEI CTNAG 2 cut(s) 200, 238
BstDSI CCRYGG 2 cut(s) 49, 647
BstF5I GGATG 3 cut(s) 145, 193, 572
BstKTI GATC 3 cut(s) 70, 281, 610
BstMAI GTCTC 2 cut(s) 12, 121
BstMBI GATC 3 cut(s) 67, 278, 607
BstMWI GCNNNNNNNGC 1 cut(s) 86
BstNI CCWGG 3 cut(s) 6, 73, 276
BstSCI CCNGG 3 cut(s) 4, 71, 274
BstSFI CTRYAG 1 cut(s) 413
BstSLI GKGCMC 2 cut(s) 275, 427
BstV1I GCAGC 1 cut(s) 298
BstX2I RGATCY 2 cut(s) 278, 607
BstYI RGATCY 2 cut(s) 278, 607
BsuRI GGCC 1 cut(s) 273
BtgI CCRYGG 2 cut(s) 49, 647
BtrI CACGTC 1 cut(s) 124
BtsCI GGATG 3 cut(s) 145, 193, 572
BtsIMutI CAGTG 2 cut(s) 246, 363
BveI ACCTGC 2 cut(s) 139, 406
Cfr13I GGNCC 2 cut(s) 271, 272
Csp6I GTAC 3 cut(s) 61, 244, 386
CviAII CATG 4 cut(s) 83, 467, 542, 648
CviJI RGCY 8 cut(s) 46, 218, 273, 292, 311, 324, 622, 672
CviKI_1 RGCY 8 cut(s) 46, 218, 273, 292, 311, 324, 622, 672
CviQI GTAC 3 cut(s) 61, 244, 386
DdeI CTNAG 2 cut(s) 200, 238
DpnI GATC 3 cut(s) 69, 280, 609
DpnII GATC 3 cut(s) 67, 278, 607
Ecl136II GAGCTC 2 cut(s) 46, 292
Eco130I CCWWGG 1 cut(s) 647
Eco24I GRGCYC 3 cut(s) 48, 275, 294
Eco31I GGTCTC 1 cut(s) 121
Eco53kI GAGCTC 2 cut(s) 46, 292
Eco57I CTGAAG 1 cut(s) 522
EcoICRI GAGCTC 2 cut(s) 46, 292
EcoO109I RGGNCCY 2 cut(s) 271, 272
EcoRII CCWGG 3 cut(s) 4, 71, 274
EcoT14I CCWWGG 1 cut(s) 647
EcoT38I GRGCYC 3 cut(s) 48, 275, 294
ErhI CCWWGG 1 cut(s) 647
Esp3I CGTCTC 1 cut(s) 12
FaeI CATG 4 cut(s) 86, 470, 545, 651
FatI CATG 4 cut(s) 82, 466, 541, 647
FauNDI CATATG 1 cut(s) 160
Fnu4HI GCNGC 1 cut(s) 312
FokI GGATG 3 cut(s) 152, 200, 559
FriOI GRGCYC 3 cut(s) 48, 275, 294
Fsp4HI GCNGC 1 cut(s) 312
FspBI CTAG 2 cut(s) 26, 315
GluI GCNGC 1 cut(s) 312
HaeIII GGCC 1 cut(s) 273
Hin1II CATG 4 cut(s) 86, 470, 545, 651
HincII GTYRAC 1 cut(s) 97
HindII GTYRAC 1 cut(s) 97
HindIII AAGCTT 2 cut(s) 216, 620
HinfI GANTC 3 cut(s) 231, 479, 506
HphI GGTGA 1 cut(s) 224
Hpy166II GTNNAC 2 cut(s) 97, 244
Hpy188I TCNGA 3 cut(s) 41, 283, 502
Hpy188III TCNNGA 2 cut(s) 119, 566
Hpy8I GTNNAC 2 cut(s) 97, 244
HpyAV CCTTC 1 cut(s) 162
HpyCH4III ACNGT 2 cut(s) 248, 666
HpyCH4IV ACGT 2 cut(s) 21, 123
HpyCH4V TGCA 8 cut(s) 134, 266, 339, 415, 451, 461, 474, 512
HpyF10VI GCNNNNNNNGC 1 cut(s) 86
HpyF3I CTNAG 2 cut(s) 200, 238
HpySE526I ACGT 2 cut(s) 21, 123
Hsp92II CATG 4 cut(s) 86, 470, 545, 651
Kzo9I GATC 3 cut(s) 67, 278, 607
LmnI GCTCC 1 cut(s) 51
Lsp1109I GCAGC 1 cut(s) 298
LweI GCATC 4 cut(s) 253, 448, 569, 581
MaeI CTAG 2 cut(s) 26, 315
MaeII ACGT 2 cut(s) 21, 123
MaeIII GTNAC 2 cut(s) 28, 628
MalI GATC 3 cut(s) 69, 280, 609
MboI GATC 3 cut(s) 67, 278, 607
MboII GAAGA 2 cut(s) 515, 602
MflI RGATCY 2 cut(s) 278, 607
MhlI GDGCHC 4 cut(s) 48, 275, 294, 427
MluCI AATT 5 cut(s) 12, 331, 398, 486, 513
MlyI GAGTC 1 cut(s) 240
MnlI CCTC 8 cut(s) 136, 160, 262, 430, 433, 508, 538, 562
MseI TTAA 2 cut(s) 401, 603
MspA1I CMGCKG 1 cut(s) 311
MspR9I CCNGG 3 cut(s) 6, 73, 276
Mva1269I GAATGC 1 cut(s) 476
MvaI CCWGG 3 cut(s) 6, 73, 276
MwoI GCNNNNNNNGC 1 cut(s) 86
NcoI CCATGG 1 cut(s) 647
NdeI CATATG 1 cut(s) 160
NdeII GATC 3 cut(s) 67, 278, 607
NlaIII CATG 4 cut(s) 86, 470, 545, 651
NlaIV GGNNCC 2 cut(s) 273, 424
PaqCI CACCTGC 1 cut(s) 406
PctI GAATGC 1 cut(s) 476
PfeI GAWTC 2 cut(s) 479, 506
PkrI GCNGC 1 cut(s) 313
PleI GAGTC 1 cut(s) 239
PpsI GAGTC 1 cut(s) 239
PsiI TTATAA 1 cut(s) 533
Psp124BI GAGCTC 2 cut(s) 48, 294
Psp6I CCWGG 3 cut(s) 4, 71, 274
PspGI CCWGG 3 cut(s) 4, 71, 274
PspN4I GGNNCC 2 cut(s) 273, 424
PspOMI GGGCCC 1 cut(s) 271
PspPI GGNCC 2 cut(s) 271, 272
PsrI GAACNNNNNNTAC 2 cut(s) 337, 369
PstI CTGCAG 1 cut(s) 417
PsuI RGATCY 2 cut(s) 278, 607
PvuII CAGCTG 1 cut(s) 311
RsaI GTAC 3 cut(s) 62, 245, 387
RsaNI GTAC 3 cut(s) 61, 244, 386
SacI GAGCTC 2 cut(s) 48, 294
SaqAI TTAA 2 cut(s) 401, 603
SatI GCNGC 1 cut(s) 312
Sau3AI GATC 3 cut(s) 67, 278, 607
Sau96I GGNCC 2 cut(s) 271, 272
SchI GAGTC 1 cut(s) 240
ScrFI CCNGG 3 cut(s) 6, 73, 276
SduI GDGCHC 4 cut(s) 48, 275, 294, 427
SfaNI GCATC 4 cut(s) 253, 448, 569, 581
SfcI CTRYAG 1 cut(s) 413
SmlI CTYRAG 1 cut(s) 566
SmoI CTYRAG 1 cut(s) 566
Sse9I AATT 5 cut(s) 12, 331, 398, 486, 513
SsiI CCGC 1 cut(s) 156
SspMI CTAG 2 cut(s) 26, 315
SstI GAGCTC 2 cut(s) 48, 294
StyD4I CCNGG 3 cut(s) 4, 71, 274
StyI CCWWGG 1 cut(s) 647
TaaI ACNGT 2 cut(s) 248, 666
TaiI ACGT 2 cut(s) 24, 126
TasI AATT 5 cut(s) 12, 331, 398, 486, 513
TatI WGTACW 2 cut(s) 243, 385
TfiI GAWTC 2 cut(s) 479, 506
Tru1I TTAA 2 cut(s) 401, 603
Tru9I TTAA 2 cut(s) 401, 603
TscAI CASTG 2 cut(s) 246, 363
TseI GCWGC 1 cut(s) 311
TspDTI ATGAA 2 cut(s) 396, 483
TspGWI ACGGA 1 cut(s) 38
TspRI CASTG 2 cut(s) 246, 363
XapI RAATTY 1 cut(s) 331
XspI CTAG 2 cut(s) 26, 315
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.