Rorug03G0009700

F-actin-capping proteins bind in a Ca(2 )-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0008545
Physical Location & Seq
Forward (+)
707434 .. 709202
1769 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0009700.1

Sequence Viewer

Length: 1131 bp
ATGGGTTGCACGTCTTCAAAATACTCCATAGAGGTATCTAATGAAGATGAAGAAAAACAGGACTCGAATGTCAGAGGGACTATAATTGTCGCCTTCTTTTGGAAGCAATTCATTCTGTTTGGTGTCGATTCTAGGGCATCGAATGAATCGCGTCAAGATATTGAAGGAGAGCATGAATGGAAGCTTAGAATCGACGACAATCATACAAAGTTGTTTGCAATATCTTCCAACATAATCGCCATAATGGTGGGAGACGCTCGTGATAGCAAAGCCATGTGCCAACATGCACTACGTTACTTACATAAACGGAAGAAGGCCCCGTTCCAAGTTGAAGATGTATTGGAAACAATGTCTTCGTACTTAACAGAGGAGCGTGACAGGGGCGAGAGCAAACGCTTTGGGGCCATGATTGTTGGATGGGATCCGATAAAGAAATTTCAAATTTGTAGTGTTAATATACGTGGCGACAGGATTGTGAAAAAAAGACACGAATATCTCGCAGCACTAGGAAGTGGAACACACTATAGTATGAAGTTCTTGGGACGTTGTTATAACTCTTCCATGTCAGAAAAAGACGCTATTGAGTTGGTCCTAAAGTCTTTATTTTATGCAACCTTGAGTGATAAGCATTGCGGTGGAATTCTGCATGTTTATCGCCTGGATGAGAGCAGAATCCATGACGAAAAGAGTCAGAATGCTATGGAGGTATATTTAGAATATTACGAGTCTTGCAATGAACGAAATACACTTTTTATCGTTTATTCTTCGAAGTACCAACCTTTTTCTGCAGATGACCTCATAACCCACTTAAGGCAATATGGAGCAATGAAAGTCGAAGCAGCTCATCTTGTTGCCCATTGTGAATCACAGAATGAAGGCATCGCGTTCTACTTCCACAGACTCATACTCGAGACAGAAAAAGAAGCTACAAAAGTTTTTAGAAACGTGGTGAAAAATGCTCTTGGATTTCCTAGTGATGGTCAATTTGTTGATGATGCCCTAGTTTTTAGAGCAATGCGATATCGAGTGCTCTTGAATACTAAAGAGATGGATGTGCCAGTTTATGTGAACAAGAGCTCGAAGCAGTTGTTGGAAGTCATAACATCAAATGCGGAAGTAATATATCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
F-actin_cap_A

Protein Analysis

376

Amino Acids

43.37

Weight (kDa)

6.68

Isoelectric Point (pI)

42.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 25 - 213 3.4e-16 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 552
AasI GACNNNNNNGTC 1 cut(s) 68
AccII CGCG 2 cut(s) 151, 884
AciI CCGC 2 cut(s) 633, 1112
AclWI GGATC 2 cut(s) 416, 429
AcsI RAATTY 3 cut(s) 434, 441, 639
AfaI GTAC 2 cut(s) 359, 773
AfiI CCNNNNNNNGG 3 cut(s) 99, 810, 977
AflII CTTAAG 1 cut(s) 808
AgsI TTSAA 5 cut(s) 18, 164, 332, 440, 1036
AjiI CACGTC 1 cut(s) 12
AjnI CCWGG 1 cut(s) 657
AjuI GAANNNNNNNTTGG 2 cut(s) 1073, 1105
AluBI AGCT 4 cut(s) 184, 842, 926, 1077
AluI AGCT 4 cut(s) 184, 842, 926, 1077
Alw21I GWGCWC 2 cut(s) 1032, 1079
Alw26I GTCTC 2 cut(s) 246, 905
AlwI GGATC 2 cut(s) 416, 429
Ama87I CYCGRG 1 cut(s) 908
AoxI GGCC 2 cut(s) 315, 402
ApeKI GCWGC 2 cut(s) 500, 839
ApoI RAATTY 3 cut(s) 434, 441, 639
Asp700I GAANNNNTTC 1 cut(s) 107
AspS9I GGNCC 3 cut(s) 316, 402, 589
AsuHPI GGTGA 1 cut(s) 961
AsuII TTCGAA 1 cut(s) 767
AvaI CYCGRG 1 cut(s) 908
AvaII GGWCC 1 cut(s) 589
BamHI GGATCC 1 cut(s) 421
BanII GRGCYC 1 cut(s) 1079
BauI CACGAG 1 cut(s) 258
BbsI GAAGAC 2 cut(s) 6, 345
Bbv12I GWGCWC 2 cut(s) 1032, 1079
BbvI GCAGC 2 cut(s) 512, 851
BccI CCATC 3 cut(s) 411, 971, 1042
BcgI CGANNNNNNTGC 2 cut(s) 635, 669
BciT130I CCWGG 1 cut(s) 659
BcoDI GTCTC 2 cut(s) 246, 905
BfaI CTAG 4 cut(s) 132, 506, 972, 1001
BfmI CTRYAG 2 cut(s) 523, 786
BfrI CTTAAG 1 cut(s) 808
BisI GCNGC 2 cut(s) 501, 840
BlsI GCNGC 2 cut(s) 502, 841
Bme1390I CCNGG 1 cut(s) 659
Bme18I GGWCC 1 cut(s) 589
BmeT110I CYCGRG 1 cut(s) 908
BmgBI CACGTC 1 cut(s) 12
BmgT120I GGNCC 3 cut(s) 316, 402, 589
BmiI GGNNCC 3 cut(s) 318, 403, 423
BmrFI CCNGG 1 cut(s) 659
BmsI GCATC 3 cut(s) 146, 888, 985
BpiI GAAGAC 2 cut(s) 6, 345
Bpu14I TTCGAA 1 cut(s) 767
BpuEI CTTGAG 1 cut(s) 637
Bsa29I ATCGAT 1 cut(s) 1126
BsaAI YACGTR 1 cut(s) 461
Bsc4I CCNNNNNNNGG 3 cut(s) 99, 810, 977
Bse1I ACTGG 1 cut(s) 1058
Bse3DI GCAATG 4 cut(s) 628, 739, 831, 1020
BseBI CCWGG 1 cut(s) 659
BseCI ATCGAT 1 cut(s) 1126
BseGI GGATG 3 cut(s) 422, 667, 1057
BseLI CCNNNNNNNGG 3 cut(s) 99, 810, 977
BseMI GCAATG 4 cut(s) 628, 739, 831, 1020
BseNI ACTGG 1 cut(s) 1058
BseRI GAGGAG 1 cut(s) 383
BseXI GCAGC 2 cut(s) 512, 851
Bsh1236I CGCG 2 cut(s) 151, 884
BshFI GGCC 2 cut(s) 317, 404
BshVI ATCGAT 1 cut(s) 1126
BsiHKAI GWGCWC 2 cut(s) 1032, 1079
BsiHKCI CYCGRG 1 cut(s) 908
BslFI GGGAC 2 cut(s) 91, 555
BslI CCNNNNNNNGG 3 cut(s) 99, 810, 977
BsmAI GTCTC 2 cut(s) 246, 905
BsmBI CGTCTC 1 cut(s) 246
BsmFI GGGAC 2 cut(s) 91, 555
BsmI GAATGC 1 cut(s) 700
BsnI GGCC 2 cut(s) 317, 404
BsoBI CYCGRG 1 cut(s) 908
Bsp119I TTCGAA 1 cut(s) 767
Bsp1286I GDGCHC 2 cut(s) 1032, 1079
Bsp143I GATC 1 cut(s) 421
BspACI CCGC 2 cut(s) 633, 1112
BspANI GGCC 2 cut(s) 317, 404
BspDI ATCGAT 1 cut(s) 1126
BspFNI CGCG 2 cut(s) 151, 884
BspLI GGNNCC 3 cut(s) 318, 403, 423
BspMAI CTGCAG 1 cut(s) 790
BspPI GGATC 2 cut(s) 416, 429
BspT104I TTCGAA 1 cut(s) 767
BspTI CTTAAG 1 cut(s) 808
BsrDI GCAATG 4 cut(s) 628, 739, 831, 1020
BsrI ACTGG 1 cut(s) 1058
BssMI GATC 1 cut(s) 421
BssSI CACGAG 1 cut(s) 258
Bst2BI CACGAG 1 cut(s) 258
Bst2UI CCWGG 1 cut(s) 659
Bst6I CTCTTC 1 cut(s) 562
BstAFI CTTAAG 1 cut(s) 808
BstBAI YACGTR 1 cut(s) 461
BstBI TTCGAA 1 cut(s) 767
BstDEI CTNAG 1 cut(s) 185
BstF5I GGATG 3 cut(s) 422, 667, 1057
BstFNI CGCG 2 cut(s) 151, 884
BstKTI GATC 1 cut(s) 424
BstMAI GTCTC 2 cut(s) 246, 905
BstMBI GATC 1 cut(s) 421
BstNI CCWGG 1 cut(s) 659
BstNSI RCATGY 2 cut(s) 287, 650
BstSCI CCNGG 1 cut(s) 657
BstSFI CTRYAG 2 cut(s) 523, 786
BstUI CGCG 2 cut(s) 151, 884
BstV1I GCAGC 2 cut(s) 512, 851
BstV2I GAAGAC 2 cut(s) 6, 345
BstX2I RGATCY 1 cut(s) 421
BstXI CCANNNNNNTGG 1 cut(s) 247
BstYI RGATCY 1 cut(s) 421
Bsu15I ATCGAT 1 cut(s) 1126
BsuRI GGCC 2 cut(s) 317, 404
BsuTUI ATCGAT 1 cut(s) 1126
BtgZI GCGATG 1 cut(s) 865
BtrI CACGTC 1 cut(s) 12
BtsCI GGATG 3 cut(s) 422, 667, 1057
Cfr13I GGNCC 3 cut(s) 316, 402, 589
ClaI ATCGAT 1 cut(s) 1126
CseI GACGC 3 cut(s) 140, 263, 584
Csp6I GTAC 2 cut(s) 358, 772
CviAII CATG 7 cut(s) 173, 274, 284, 406, 562, 647, 677
CviJI RGCY 7 cut(s) 184, 272, 317, 404, 842, 926, 1077
CviKI_1 RGCY 7 cut(s) 184, 272, 317, 404, 842, 926, 1077
CviQI GTAC 2 cut(s) 358, 772
DdeI CTNAG 1 cut(s) 185
DpnI GATC 1 cut(s) 423
DpnII GATC 1 cut(s) 421
DrdI GACNNNNNNGTC 1 cut(s) 68
DseDI GACNNNNNNGTC 1 cut(s) 68
Eam1104I CTCTTC 1 cut(s) 562
EarI CTCTTC 1 cut(s) 562
Ecl136II GAGCTC 1 cut(s) 1077
Eco24I GRGCYC 1 cut(s) 1079
Eco32I GATATC 1 cut(s) 1022
Eco47I GGWCC 1 cut(s) 589
Eco53kI GAGCTC 1 cut(s) 1077
Eco88I CYCGRG 1 cut(s) 908
EcoICRI GAGCTC 1 cut(s) 1077
EcoO109I RGGNCCY 1 cut(s) 316
EcoRI GAATTC 1 cut(s) 639
EcoRII CCWGG 1 cut(s) 657
EcoRV GATATC 1 cut(s) 1022
EcoT38I GRGCYC 1 cut(s) 1079
Esp3I CGTCTC 1 cut(s) 246
FaeI CATG 7 cut(s) 176, 277, 287, 409, 565, 650, 680
FaqI GGGAC 2 cut(s) 91, 555
FatI CATG 7 cut(s) 172, 273, 283, 405, 561, 646, 676
Fnu4HI GCNGC 2 cut(s) 501, 840
FokI GGATG 3 cut(s) 429, 674, 1064
FriOI GRGCYC 1 cut(s) 1079
Fsp4HI GCNGC 2 cut(s) 501, 840
FspBI CTAG 4 cut(s) 132, 506, 972, 1001
GluI GCNGC 2 cut(s) 501, 840
HaeIII GGCC 2 cut(s) 317, 404
HgaI GACGC 3 cut(s) 140, 263, 584
Hin1II CATG 7 cut(s) 176, 277, 287, 409, 565, 650, 680
HindIII AAGCTT 1 cut(s) 182
HinfI GANTC 9 cut(s) 62, 128, 146, 189, 672, 688, 725, 863, 900
HphI GGTGA 1 cut(s) 961
Hpy166II GTNNAC 1 cut(s) 1069
Hpy188I TCNGA 4 cut(s) 74, 426, 568, 693
Hpy188III TCNNGA 4 cut(s) 155, 260, 910, 1033
Hpy8I GTNNAC 1 cut(s) 1069
Hpy99I CGWCG 1 cut(s) 197
HpyAV CCTTC 4 cut(s) 103, 158, 307, 869
HpyCH4IV ACGT 5 cut(s) 11, 292, 460, 544, 945
HpyCH4V TGCA 7 cut(s) 9, 218, 287, 611, 646, 732, 788
HpyF3I CTNAG 1 cut(s) 185
HpySE526I ACGT 5 cut(s) 11, 292, 460, 544, 945
Hsp92II CATG 7 cut(s) 176, 277, 287, 409, 565, 650, 680
Kzo9I GATC 1 cut(s) 421
LmnI GCTCC 2 cut(s) 370, 821
LpnPI CCDG 6 cut(s) 44, 364, 454, 644, 671, 1071
Lsp1109I GCAGC 2 cut(s) 512, 851
LweI GCATC 3 cut(s) 146, 888, 985
MaeI CTAG 4 cut(s) 132, 506, 972, 1001
MaeII ACGT 5 cut(s) 11, 292, 460, 544, 945
MaeIII GTNAC 2 cut(s) 293, 374
MalI GATC 1 cut(s) 423
MboI GATC 1 cut(s) 421
MboII GAAGA 9 cut(s) 6, 56, 62, 216, 322, 344, 345, 549, 756
MflI RGATCY 1 cut(s) 421
MhlI GDGCHC 2 cut(s) 1032, 1079
MluCI AATT 6 cut(s) 84, 107, 434, 441, 639, 983
MlyI GAGTC 4 cut(s) 56, 697, 734, 894
MmeI TCCRAC 3 cut(s) 252, 394, 1071
MnlI CCTC 5 cut(s) 25, 68, 361, 697, 806
MroXI GAANNNNTTC 1 cut(s) 107
MseI TTAA 3 cut(s) 362, 453, 809
MslI CAYNNNNRTG 2 cut(s) 245, 633
MspCI CTTAAG 1 cut(s) 808
MspR9I CCNGG 1 cut(s) 659
Mva1269I GAATGC 1 cut(s) 700
MvaI CCWGG 1 cut(s) 659
MvnI CGCG 2 cut(s) 151, 884
NdeII GATC 1 cut(s) 421
NlaIII CATG 7 cut(s) 176, 277, 287, 409, 565, 650, 680
NlaIV GGNNCC 3 cut(s) 318, 403, 423
NmuCI GTSAC 1 cut(s) 374
NspI RCATGY 2 cut(s) 287, 650
NspV TTCGAA 1 cut(s) 767
PaeR7I CTCGAG 1 cut(s) 908
PctI GAATGC 1 cut(s) 700
PdmI GAANNNNTTC 1 cut(s) 107
PfeI GAWTC 5 cut(s) 128, 146, 189, 672, 863
PkrI GCNGC 2 cut(s) 502, 841
PleI GAGTC 4 cut(s) 56, 696, 733, 894
PpsI GAGTC 4 cut(s) 56, 696, 733, 894
Ppu21I YACGTR 1 cut(s) 461
PsiI TTATAA 1 cut(s) 552
Psp124BI GAGCTC 1 cut(s) 1079
Psp6I CCWGG 1 cut(s) 657
PspGI CCWGG 1 cut(s) 657
PspN4I GGNNCC 3 cut(s) 318, 403, 423
PspPI GGNCC 3 cut(s) 316, 402, 589
PstI CTGCAG 1 cut(s) 790
PsuI RGATCY 1 cut(s) 421
RsaI GTAC 2 cut(s) 359, 773
RsaNI GTAC 2 cut(s) 358, 772
RseI CAYNNNNRTG 2 cut(s) 245, 633
SacI GAGCTC 1 cut(s) 1079
SaqAI TTAA 3 cut(s) 362, 453, 809
SatI GCNGC 2 cut(s) 501, 840
Sau3AI GATC 1 cut(s) 421
Sau96I GGNCC 3 cut(s) 316, 402, 589
SchI GAGTC 4 cut(s) 56, 697, 734, 894
ScrFI CCNGG 1 cut(s) 659
SduI GDGCHC 2 cut(s) 1032, 1079
SfaNI GCATC 3 cut(s) 146, 888, 985
SfcI CTRYAG 2 cut(s) 523, 786
Sfr274I CTCGAG 1 cut(s) 908
SfuI TTCGAA 1 cut(s) 767
SinI GGWCC 1 cut(s) 589
SlaI CTCGAG 1 cut(s) 908
SmiMI CAYNNNNRTG 2 cut(s) 245, 633
SmlI CTYRAG 3 cut(s) 616, 808, 908
SmoI CTYRAG 3 cut(s) 616, 808, 908
Sse9I AATT 6 cut(s) 84, 107, 434, 441, 639, 983
SsiI CCGC 2 cut(s) 633, 1112
SspI AATATT 1 cut(s) 719
SspMI CTAG 4 cut(s) 132, 506, 972, 1001
SstI GAGCTC 1 cut(s) 1079
StyD4I CCNGG 1 cut(s) 657
TaiI ACGT 5 cut(s) 14, 295, 463, 547, 948
TasI AATT 6 cut(s) 84, 107, 434, 441, 639, 983
TfiI GAWTC 5 cut(s) 128, 146, 189, 672, 863
Tru1I TTAA 3 cut(s) 362, 453, 809
Tru9I TTAA 3 cut(s) 362, 453, 809
TseFI GTSAC 1 cut(s) 374
TseI GCWGC 2 cut(s) 500, 839
Tsp45I GTSAC 1 cut(s) 374
TspDTI ATGAA 9 cut(s) 57, 63, 100, 159, 189, 545, 750, 842, 888
TspGWI ACGGA 1 cut(s) 322
Vha464I CTTAAG 1 cut(s) 808
VpaK11BI GGWCC 1 cut(s) 589
XapI RAATTY 3 cut(s) 434, 441, 639
XceI RCATGY 2 cut(s) 287, 650
XhoI CTCGAG 1 cut(s) 908
XmnI GAANNNNTTC 1 cut(s) 107
XspI CTAG 4 cut(s) 132, 506, 972, 1001
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.