Rh3DG072500

F-actin-capping proteins bind in a Ca(2 )-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0008545
Physical Location & Seq
Forward (+)
4902800 .. 4906704
3905 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG072500.1

Sequence Viewer

Length: 702 bp
ATGCATGCGCCTTCTATTCTCCTTGTTTTGTTTGTGCCACATTTTTTTTTTTTTTGGATCTTTTCATTTACCAATTATTTAATGCTAAAATTGGTTCAGGTTTGTCCAGACGTGAGACCTGCATTGGATGAGGAACTTCCTTCCGCATATGTTGAGGAATACAGGTGTGCTTTGGATGGAGAAATACTTAGATATGTGGGTGAAGCTTATCCAAAAGGGGTCTGCTCAGTGTACTGTGGGAATGGTAAAGATGCAGAGGGCCCAGGATCTGACTTTGAGCTCGTGGTAGTGATTTCAGCTGCTAGACATAGCCCACAAAATTTCTGCAATGGTAGTTGGCGTTCAGTGTGGAGTATTGAGTTCAAAGATGAAGTACAAGTGCTGGAATTAAAAGGCAAACTGCAGGTGGGTGCCCACTATTTTGAGGAGGGGAATGTGCAGTTAGATGCAAAACATGAATGCAAAGATTCAACAATTTTTCAGTCCTCTGAAGATTCTGCAATTACCATAGGCAACATTATAAGGCAACACGAGGCAGAATACTTGGCATCTCTTGAGGCATCCTATTCAAACTTGCCCGATACCACTTTTAAGGATCTTCGTAGAAAGCTTCCAGTTACTCGCACCCTATTTCCATGGCAGAATACTTTACAGTTTAGCTTGACAAGAGATATCACAAAAGAACTTGGCATTGGAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
F-actin_cap_A

Protein Analysis

233

Amino Acids

26.44

Weight (kDa)

5.18

Isoelectric Point (pI)

40.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-actin_cap_A PF01267 46 - 224 1.1e-51 F-actin capping protein alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 521
AarI CACCTGC 1 cut(s) 394
Acc36I ACCTGC 2 cut(s) 127, 394
AccB1I GGYRCC 1 cut(s) 410
AciI CCGC 1 cut(s) 144
AclWI GGATC 3 cut(s) 65, 274, 603
AcsI RAATTY 1 cut(s) 319
AcuI CTGAAG 1 cut(s) 510
AfaI GTAC 2 cut(s) 233, 375
AgsI TTSAA 3 cut(s) 364, 471, 570
AjiI CACGTC 1 cut(s) 112
AjnI CCWGG 1 cut(s) 262
AjuI GAANNNNNNNTTGG 1 cut(s) 675
AluBI AGCT 5 cut(s) 206, 280, 299, 610, 660
AluI AGCT 5 cut(s) 206, 280, 299, 610, 660
Alw21I GWGCWC 1 cut(s) 282
Alw26I GTCTC 1 cut(s) 109
AlwI GGATC 3 cut(s) 65, 274, 603
AlwNI CAGNNNCTG 1 cut(s) 269
AoxI GGCC 1 cut(s) 259
ApaI GGGCCC 1 cut(s) 263
ApeKI GCWGC 1 cut(s) 299
ApoI RAATTY 1 cut(s) 319
AspLEI GCGC 1 cut(s) 10
AspS9I GGNCC 2 cut(s) 259, 260
AsuHPI GGTGA 1 cut(s) 212
BaeGI GKGCMC 2 cut(s) 263, 415
BanI GGYRCC 1 cut(s) 410
BanII GRGCYC 2 cut(s) 263, 282
BauI CACGAG 2 cut(s) 281, 530
Bbv12I GWGCWC 1 cut(s) 282
BbvI GCAGC 1 cut(s) 286
BccI CCATC 1 cut(s) 170
BciT130I CCWGG 1 cut(s) 264
BcoDI GTCTC 1 cut(s) 109
BfaI CTAG 1 cut(s) 303
BfmI CTRYAG 1 cut(s) 401
BfuAI ACCTGC 2 cut(s) 127, 394
BisI GCNGC 1 cut(s) 300
BlsI GCNGC 1 cut(s) 301
Bme1390I CCNGG 1 cut(s) 264
BmgBI CACGTC 1 cut(s) 112
BmgT120I GGNCC 2 cut(s) 259, 260
BmiI GGNNCC 2 cut(s) 261, 412
BmrFI CCNGG 1 cut(s) 264
BmsI GCATC 4 cut(s) 241, 436, 557, 569
BpuEI CTTGAG 1 cut(s) 575
BsaI GGTCTC 1 cut(s) 109
BsaJI CCNNGG 2 cut(s) 262, 635
Bse1I ACTGG 1 cut(s) 614
Bse3DI GCAATG 1 cut(s) 334
BseBI CCWGG 1 cut(s) 264
BseDI CCNNGG 2 cut(s) 262, 635
BseGI GGATG 3 cut(s) 133, 181, 560
BseMI GCAATG 1 cut(s) 334
BseMII CTCAG 1 cut(s) 240
BseNI ACTGG 1 cut(s) 614
BseRI GAGGAG 1 cut(s) 440
BseSI GKGCMC 2 cut(s) 263, 415
BseXI GCAGC 1 cut(s) 286
BsgI GTGCAG 1 cut(s) 458
BshFI GGCC 1 cut(s) 261
BshNI GGYRCC 1 cut(s) 410
BsiHKAI GWGCWC 1 cut(s) 282
BsmAI GTCTC 1 cut(s) 109
BsmI GAATGC 1 cut(s) 464
BsnI GGCC 1 cut(s) 261
Bso31I GGTCTC 1 cut(s) 109
Bsp120I GGGCCC 1 cut(s) 259
Bsp1286I GDGCHC 3 cut(s) 263, 282, 415
Bsp143I GATC 3 cut(s) 57, 266, 595
Bsp19I CCATGG 1 cut(s) 635
BspACI CCGC 1 cut(s) 144
BspANI GGCC 1 cut(s) 261
BspCNI CTCAG 1 cut(s) 239
BspLI GGNNCC 2 cut(s) 261, 412
BspMAI CTGCAG 1 cut(s) 405
BspMI ACCTGC 2 cut(s) 127, 394
BspPI GGATC 3 cut(s) 65, 274, 603
BspT107I GGYRCC 1 cut(s) 410
BspTNI GGTCTC 1 cut(s) 109
BsrDI GCAATG 1 cut(s) 334
BsrI ACTGG 1 cut(s) 614
BssECI CCNNGG 2 cut(s) 262, 635
BssMI GATC 3 cut(s) 57, 266, 595
BssSI CACGAG 2 cut(s) 281, 530
BssT1I CCWWGG 1 cut(s) 635
Bst2BI CACGAG 2 cut(s) 281, 530
Bst2UI CCWGG 1 cut(s) 264
Bst4CI ACNGT 2 cut(s) 236, 654
BstC8I GCNNGC 1 cut(s) 6
BstDEI CTNAG 2 cut(s) 188, 226
BstDSI CCRYGG 1 cut(s) 635
BstF5I GGATG 3 cut(s) 133, 181, 560
BstHHI GCGC 1 cut(s) 10
BstKTI GATC 3 cut(s) 60, 269, 598
BstMAI GTCTC 1 cut(s) 109
BstMBI GATC 3 cut(s) 57, 266, 595
BstNI CCWGG 1 cut(s) 264
BstNSI RCATGY 1 cut(s) 8
BstSCI CCNGG 1 cut(s) 262
BstSFI CTRYAG 1 cut(s) 401
BstSLI GKGCMC 2 cut(s) 263, 415
BstV1I GCAGC 1 cut(s) 286
BstX2I RGATCY 3 cut(s) 57, 266, 595
BstYI RGATCY 3 cut(s) 57, 266, 595
BsuRI GGCC 1 cut(s) 261
BtgI CCRYGG 1 cut(s) 635
BtrI CACGTC 1 cut(s) 112
BtsCI GGATG 3 cut(s) 133, 181, 560
BtsIMutI CAGTG 2 cut(s) 234, 351
BveI ACCTGC 2 cut(s) 127, 394
Cac8I GCNNGC 1 cut(s) 6
CaiI CAGNNNCTG 1 cut(s) 269
CfoI GCGC 1 cut(s) 10
Cfr13I GGNCC 2 cut(s) 259, 260
Csp6I GTAC 2 cut(s) 232, 374
CviAII CATG 3 cut(s) 5, 455, 636
CviJI RGCY 7 cut(s) 206, 261, 280, 299, 312, 610, 660
CviKI_1 RGCY 7 cut(s) 206, 261, 280, 299, 312, 610, 660
CviQI GTAC 2 cut(s) 232, 374
DdeI CTNAG 2 cut(s) 188, 226
DpnI GATC 3 cut(s) 59, 268, 597
DpnII GATC 3 cut(s) 57, 266, 595
Ecl136II GAGCTC 1 cut(s) 280
Eco130I CCWWGG 1 cut(s) 635
Eco24I GRGCYC 2 cut(s) 263, 282
Eco31I GGTCTC 1 cut(s) 109
Eco32I GATATC 1 cut(s) 673
Eco53kI GAGCTC 1 cut(s) 280
Eco57I CTGAAG 1 cut(s) 510
EcoICRI GAGCTC 1 cut(s) 280
EcoO109I RGGNCCY 1 cut(s) 259
EcoRII CCWGG 1 cut(s) 262
EcoRV GATATC 1 cut(s) 673
EcoT14I CCWWGG 1 cut(s) 635
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 2 cut(s) 263, 282
ErhI CCWWGG 1 cut(s) 635
FaeI CATG 3 cut(s) 8, 458, 639
FaiI YATR 9 cut(s) 6, 148, 150, 195, 309, 456, 509, 521, 637
FatI CATG 3 cut(s) 4, 454, 635
FauNDI CATATG 1 cut(s) 148
Fnu4HI GCNGC 1 cut(s) 300
FokI GGATG 3 cut(s) 140, 188, 547
FriOI GRGCYC 2 cut(s) 263, 282
Fsp4HI GCNGC 1 cut(s) 300
FspBI CTAG 1 cut(s) 303
GlaI GCGC 1 cut(s) 9
GluI GCNGC 1 cut(s) 300
HaeIII GGCC 1 cut(s) 261
HhaI GCGC 1 cut(s) 10
Hin1II CATG 3 cut(s) 8, 458, 639
Hin6I GCGC 1 cut(s) 8
HinP1I GCGC 1 cut(s) 8
HindIII AAGCTT 2 cut(s) 204, 608
HinfI GANTC 2 cut(s) 467, 494
HphI GGTGA 1 cut(s) 212
Hpy166II GTNNAC 1 cut(s) 232
Hpy188I TCNGA 2 cut(s) 271, 490
Hpy188III TCNNGA 2 cut(s) 107, 554
Hpy8I GTNNAC 1 cut(s) 232
HpyAV CCTTC 2 cut(s) 21, 150
HpyCH4III ACNGT 2 cut(s) 236, 654
HpyCH4IV ACGT 1 cut(s) 111
HpyCH4V TGCA 9 cut(s) 4, 122, 254, 327, 403, 439, 449, 462, 500
HpyF3I CTNAG 2 cut(s) 188, 226
HpySE526I ACGT 1 cut(s) 111
Hsp92II CATG 3 cut(s) 8, 458, 639
HspAI GCGC 1 cut(s) 8
Kzo9I GATC 3 cut(s) 57, 266, 595
LpnPI CCDG 9 cut(s) 83, 120, 132, 148, 249, 276, 368, 389, 627
Lsp1109I GCAGC 1 cut(s) 286
LweI GCATC 4 cut(s) 241, 436, 557, 569
MaeI CTAG 1 cut(s) 303
MaeII ACGT 1 cut(s) 111
MaeIII GTNAC 1 cut(s) 616
MalI GATC 3 cut(s) 59, 268, 597
MboI GATC 3 cut(s) 57, 266, 595
MboII GAAGA 2 cut(s) 503, 590
MflI RGATCY 3 cut(s) 57, 266, 595
MhlI GDGCHC 3 cut(s) 263, 282, 415
MluCI AATT 6 cut(s) 73, 89, 319, 386, 474, 501
MnlI CCTC 8 cut(s) 124, 148, 250, 418, 421, 496, 526, 550
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 3 cut(s) 80, 389, 591
MspA1I CMGCKG 1 cut(s) 299
MspR9I CCNGG 1 cut(s) 264
Mva1269I GAATGC 1 cut(s) 464
MvaI CCWGG 1 cut(s) 264
NcoI CCATGG 1 cut(s) 635
NdeI CATATG 1 cut(s) 148
NdeII GATC 3 cut(s) 57, 266, 595
NlaIII CATG 3 cut(s) 8, 458, 639
NlaIV GGNNCC 2 cut(s) 261, 412
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 8
PaeI GCATGC 1 cut(s) 8
PaqCI CACCTGC 1 cut(s) 394
PctI GAATGC 1 cut(s) 464
PfeI GAWTC 2 cut(s) 467, 494
PkrI GCNGC 1 cut(s) 301
PsiI TTATAA 1 cut(s) 521
Psp124BI GAGCTC 1 cut(s) 282
Psp6I CCWGG 1 cut(s) 262
PspGI CCWGG 1 cut(s) 262
PspN4I GGNNCC 2 cut(s) 261, 412
PspOMI GGGCCC 1 cut(s) 259
PspPI GGNCC 2 cut(s) 259, 260
PsrI GAACNNNNNNTAC 2 cut(s) 325, 357
PstI CTGCAG 1 cut(s) 405
PstNI CAGNNNCTG 1 cut(s) 269
PsuI RGATCY 3 cut(s) 57, 266, 595
PvuII CAGCTG 1 cut(s) 299
RsaI GTAC 2 cut(s) 233, 375
RsaNI GTAC 2 cut(s) 232, 374
SacI GAGCTC 1 cut(s) 282
SaqAI TTAA 3 cut(s) 80, 389, 591
SatI GCNGC 1 cut(s) 300
Sau3AI GATC 3 cut(s) 57, 266, 595
Sau96I GGNCC 2 cut(s) 259, 260
ScrFI CCNGG 1 cut(s) 264
SduI GDGCHC 3 cut(s) 263, 282, 415
SfaNI GCATC 4 cut(s) 241, 436, 557, 569
SfcI CTRYAG 1 cut(s) 401
SmlI CTYRAG 1 cut(s) 554
SmoI CTYRAG 1 cut(s) 554
SphI GCATGC 1 cut(s) 8
Sse9I AATT 6 cut(s) 73, 89, 319, 386, 474, 501
SsiI CCGC 1 cut(s) 144
SspMI CTAG 1 cut(s) 303
SstI GAGCTC 1 cut(s) 282
StyD4I CCNGG 1 cut(s) 262
StyI CCWWGG 1 cut(s) 635
TaaI ACNGT 2 cut(s) 236, 654
TaiI ACGT 1 cut(s) 114
TasI AATT 6 cut(s) 73, 89, 319, 386, 474, 501
TatI WGTACW 2 cut(s) 231, 373
TfiI GAWTC 2 cut(s) 467, 494
Tru1I TTAA 3 cut(s) 80, 389, 591
Tru9I TTAA 3 cut(s) 80, 389, 591
TscAI CASTG 2 cut(s) 234, 351
TseI GCWGC 1 cut(s) 299
TspDTI ATGAA 3 cut(s) 54, 384, 471
TspRI CASTG 2 cut(s) 234, 351
XapI RAATTY 1 cut(s) 319
XceI RCATGY 1 cut(s) 8
XspI CTAG 1 cut(s) 303
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.