Rh3AG069400

F-actin-capping proteins bind in a Ca(2 )-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0008545
Physical Location & Seq
Forward (+)
4822962 .. 4825338
2377 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG069400.1

Sequence Viewer

Length: 699 bp
ATGTGCCTTCTATTCTCCTTGTTTTGTTTGTGTCACATTTTTTTTTTTTTTTGGATCTTTTCATTTACCAATTATTTAATGCTAAAATTGGTTCAGGTTTGTCCAGACGTGAGACCTGCATTGGATGAGGAACTTCCTTCCGCATATGTTGAGGAATTCAGGTGTGCTTTGGATGGAGAAATACTTAGATATGTGGGTGAAGCTTATCCAAAAGGAGTCTGCTCAGTGTACTGTGGGAATGGTAAAGATGCAGAGGGCCCAGGATCTGACTTTGAGCTCGTGGTAGTGATTTCAGCTGCTAGACATAGCCCACAAAATTTCTGCAATGGTAGTTGGCGTTCAGTGTGGAATATTGAGTTCAAAGATGAAGTACAAGTGCTGGAATTAAAAGGCAAACTGCAGGTGGGTGCCCACTATTTTGAGGAGGGGAATGTGCAGTTAGATGCAAAACATGAATGCAAAGATTCAACAATTTTTCAGTCCTCTGAAGATTCTGCAATTACCATAGGCAACATTATAAGGCAACACGAGGCAGAATACTTGGCATCTCTTGAGGCATCCTATTCAAACTTGCCCGATACCACTTTTAAGGATCTTCGTAGAAAGCTTCCAGTTACTCGCACCCTATTTCCATGGCAGAATACTTTACAGTTTAGCTTGACAAGAGATATCACAAAAGAACTTGGCATTGGAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
F-actin_cap_A

Protein Analysis

232

Amino Acids

26.42

Weight (kDa)

5.09

Isoelectric Point (pI)

43.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-actin_cap_A PF01267 45 - 223 1.3e-51 F-actin capping protein alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 518
AarI CACCTGC 1 cut(s) 391
Acc36I ACCTGC 2 cut(s) 124, 391
AccB1I GGYRCC 1 cut(s) 407
AciI CCGC 1 cut(s) 141
AclWI GGATC 3 cut(s) 62, 271, 600
AcsI RAATTY 2 cut(s) 155, 316
AcuI CTGAAG 1 cut(s) 507
AfaI GTAC 2 cut(s) 230, 372
AgsI TTSAA 3 cut(s) 361, 468, 567
AjiI CACGTC 1 cut(s) 109
AjnI CCWGG 1 cut(s) 259
AjuI GAANNNNNNNTTGG 1 cut(s) 672
AluBI AGCT 5 cut(s) 203, 277, 296, 607, 657
AluI AGCT 5 cut(s) 203, 277, 296, 607, 657
Alw21I GWGCWC 1 cut(s) 279
Alw26I GTCTC 1 cut(s) 106
AlwI GGATC 3 cut(s) 62, 271, 600
AlwNI CAGNNNCTG 1 cut(s) 266
AoxI GGCC 1 cut(s) 256
ApaI GGGCCC 1 cut(s) 260
ApeKI GCWGC 1 cut(s) 296
ApoI RAATTY 2 cut(s) 155, 316
AspS9I GGNCC 2 cut(s) 256, 257
AsuHPI GGTGA 1 cut(s) 209
BaeGI GKGCMC 2 cut(s) 260, 412
BanI GGYRCC 1 cut(s) 407
BanII GRGCYC 2 cut(s) 260, 279
BauI CACGAG 2 cut(s) 278, 527
Bbv12I GWGCWC 1 cut(s) 279
BbvI GCAGC 1 cut(s) 283
BccI CCATC 1 cut(s) 167
BciT130I CCWGG 1 cut(s) 261
BcoDI GTCTC 1 cut(s) 106
BfaI CTAG 1 cut(s) 300
BfmI CTRYAG 1 cut(s) 398
BfuAI ACCTGC 2 cut(s) 124, 391
BisI GCNGC 1 cut(s) 297
BlsI GCNGC 1 cut(s) 298
Bme1390I CCNGG 1 cut(s) 261
BmgBI CACGTC 1 cut(s) 109
BmgT120I GGNCC 2 cut(s) 256, 257
BmiI GGNNCC 2 cut(s) 258, 409
BmrFI CCNGG 1 cut(s) 261
BmsI GCATC 4 cut(s) 238, 433, 554, 566
BpuEI CTTGAG 1 cut(s) 572
BsaI GGTCTC 1 cut(s) 106
BsaJI CCNNGG 2 cut(s) 259, 632
Bse1I ACTGG 1 cut(s) 611
Bse3DI GCAATG 1 cut(s) 331
BseBI CCWGG 1 cut(s) 261
BseDI CCNNGG 2 cut(s) 259, 632
BseGI GGATG 3 cut(s) 130, 178, 557
BseMI GCAATG 1 cut(s) 331
BseMII CTCAG 1 cut(s) 237
BseNI ACTGG 1 cut(s) 611
BseRI GAGGAG 1 cut(s) 437
BseSI GKGCMC 2 cut(s) 260, 412
BseXI GCAGC 1 cut(s) 283
BsgI GTGCAG 1 cut(s) 455
BshFI GGCC 1 cut(s) 258
BshNI GGYRCC 1 cut(s) 407
BsiHKAI GWGCWC 1 cut(s) 279
BsmAI GTCTC 1 cut(s) 106
BsmI GAATGC 1 cut(s) 461
BsnI GGCC 1 cut(s) 258
Bso31I GGTCTC 1 cut(s) 106
Bsp120I GGGCCC 1 cut(s) 256
Bsp1286I GDGCHC 3 cut(s) 260, 279, 412
Bsp143I GATC 3 cut(s) 54, 263, 592
Bsp19I CCATGG 1 cut(s) 632
BspACI CCGC 1 cut(s) 141
BspANI GGCC 1 cut(s) 258
BspCNI CTCAG 1 cut(s) 236
BspLI GGNNCC 2 cut(s) 258, 409
BspMAI CTGCAG 1 cut(s) 402
BspMI ACCTGC 2 cut(s) 124, 391
BspPI GGATC 3 cut(s) 62, 271, 600
BspT107I GGYRCC 1 cut(s) 407
BspTNI GGTCTC 1 cut(s) 106
BsrDI GCAATG 1 cut(s) 331
BsrI ACTGG 1 cut(s) 611
BssECI CCNNGG 2 cut(s) 259, 632
BssMI GATC 3 cut(s) 54, 263, 592
BssSI CACGAG 2 cut(s) 278, 527
BssT1I CCWWGG 1 cut(s) 632
Bst2BI CACGAG 2 cut(s) 278, 527
Bst2UI CCWGG 1 cut(s) 261
Bst4CI ACNGT 2 cut(s) 233, 651
BstDEI CTNAG 2 cut(s) 185, 223
BstDSI CCRYGG 1 cut(s) 632
BstF5I GGATG 3 cut(s) 130, 178, 557
BstKTI GATC 3 cut(s) 57, 266, 595
BstMAI GTCTC 1 cut(s) 106
BstMBI GATC 3 cut(s) 54, 263, 592
BstNI CCWGG 1 cut(s) 261
BstSCI CCNGG 1 cut(s) 259
BstSFI CTRYAG 1 cut(s) 398
BstSLI GKGCMC 2 cut(s) 260, 412
BstV1I GCAGC 1 cut(s) 283
BstX2I RGATCY 3 cut(s) 54, 263, 592
BstYI RGATCY 3 cut(s) 54, 263, 592
BsuRI GGCC 1 cut(s) 258
BtgI CCRYGG 1 cut(s) 632
BtrI CACGTC 1 cut(s) 109
BtsCI GGATG 3 cut(s) 130, 178, 557
BtsIMutI CAGTG 2 cut(s) 231, 348
BveI ACCTGC 2 cut(s) 124, 391
CaiI CAGNNNCTG 1 cut(s) 266
Cfr13I GGNCC 2 cut(s) 256, 257
Csp6I GTAC 2 cut(s) 229, 371
CviAII CATG 2 cut(s) 452, 633
CviJI RGCY 7 cut(s) 203, 258, 277, 296, 309, 607, 657
CviKI_1 RGCY 7 cut(s) 203, 258, 277, 296, 309, 607, 657
CviQI GTAC 2 cut(s) 229, 371
DdeI CTNAG 2 cut(s) 185, 223
DpnI GATC 3 cut(s) 56, 265, 594
DpnII GATC 3 cut(s) 54, 263, 592
Ecl136II GAGCTC 1 cut(s) 277
Eco130I CCWWGG 1 cut(s) 632
Eco24I GRGCYC 2 cut(s) 260, 279
Eco31I GGTCTC 1 cut(s) 106
Eco32I GATATC 1 cut(s) 670
Eco53kI GAGCTC 1 cut(s) 277
Eco57I CTGAAG 1 cut(s) 507
EcoICRI GAGCTC 1 cut(s) 277
EcoO109I RGGNCCY 1 cut(s) 256
EcoRI GAATTC 1 cut(s) 155
EcoRII CCWGG 1 cut(s) 259
EcoRV GATATC 1 cut(s) 670
EcoT14I CCWWGG 1 cut(s) 632
EcoT38I GRGCYC 2 cut(s) 260, 279
ErhI CCWWGG 1 cut(s) 632
FaeI CATG 2 cut(s) 455, 636
FaiI YATR 8 cut(s) 145, 147, 192, 306, 453, 506, 518, 634
FatI CATG 2 cut(s) 451, 632
FauNDI CATATG 1 cut(s) 145
Fnu4HI GCNGC 1 cut(s) 297
FokI GGATG 3 cut(s) 137, 185, 544
FriOI GRGCYC 2 cut(s) 260, 279
Fsp4HI GCNGC 1 cut(s) 297
FspBI CTAG 1 cut(s) 300
GluI GCNGC 1 cut(s) 297
HaeIII GGCC 1 cut(s) 258
Hin1II CATG 2 cut(s) 455, 636
HindIII AAGCTT 2 cut(s) 201, 605
HinfI GANTC 3 cut(s) 216, 464, 491
HphI GGTGA 1 cut(s) 209
Hpy166II GTNNAC 1 cut(s) 229
Hpy188I TCNGA 2 cut(s) 268, 487
Hpy188III TCNNGA 2 cut(s) 104, 551
Hpy8I GTNNAC 1 cut(s) 229
HpyAV CCTTC 2 cut(s) 17, 147
HpyCH4III ACNGT 2 cut(s) 233, 651
HpyCH4IV ACGT 1 cut(s) 108
HpyCH4V TGCA 8 cut(s) 119, 251, 324, 400, 436, 446, 459, 497
HpyF3I CTNAG 2 cut(s) 185, 223
HpySE526I ACGT 1 cut(s) 108
Hsp92II CATG 2 cut(s) 455, 636
Kzo9I GATC 3 cut(s) 54, 263, 592
LpnPI CCDG 9 cut(s) 80, 117, 129, 145, 246, 273, 365, 386, 624
Lsp1109I GCAGC 1 cut(s) 283
LweI GCATC 4 cut(s) 238, 433, 554, 566
MaeI CTAG 1 cut(s) 300
MaeII ACGT 1 cut(s) 108
MaeIII GTNAC 2 cut(s) 32, 613
MalI GATC 3 cut(s) 56, 265, 594
MboI GATC 3 cut(s) 54, 263, 592
MboII GAAGA 2 cut(s) 500, 587
MflI RGATCY 3 cut(s) 54, 263, 592
MhlI GDGCHC 3 cut(s) 260, 279, 412
MluCI AATT 7 cut(s) 70, 86, 155, 316, 383, 471, 498
MlyI GAGTC 1 cut(s) 225
MnlI CCTC 8 cut(s) 121, 145, 247, 415, 418, 493, 523, 547
MseI TTAA 3 cut(s) 77, 386, 588
MspA1I CMGCKG 1 cut(s) 296
MspR9I CCNGG 1 cut(s) 261
Mva1269I GAATGC 1 cut(s) 461
MvaI CCWGG 1 cut(s) 261
NcoI CCATGG 1 cut(s) 632
NdeI CATATG 1 cut(s) 145
NdeII GATC 3 cut(s) 54, 263, 592
NlaIII CATG 2 cut(s) 455, 636
NlaIV GGNNCC 2 cut(s) 258, 409
NmuCI GTSAC 1 cut(s) 32
PaqCI CACCTGC 1 cut(s) 391
PctI GAATGC 1 cut(s) 461
PfeI GAWTC 2 cut(s) 464, 491
PkrI GCNGC 1 cut(s) 298
PleI GAGTC 1 cut(s) 224
PpsI GAGTC 1 cut(s) 224
PsiI TTATAA 1 cut(s) 518
Psp124BI GAGCTC 1 cut(s) 279
Psp6I CCWGG 1 cut(s) 259
PspGI CCWGG 1 cut(s) 259
PspN4I GGNNCC 2 cut(s) 258, 409
PspOMI GGGCCC 1 cut(s) 256
PspPI GGNCC 2 cut(s) 256, 257
PsrI GAACNNNNNNTAC 2 cut(s) 322, 354
PstI CTGCAG 1 cut(s) 402
PstNI CAGNNNCTG 1 cut(s) 266
PsuI RGATCY 3 cut(s) 54, 263, 592
PvuII CAGCTG 1 cut(s) 296
RsaI GTAC 2 cut(s) 230, 372
RsaNI GTAC 2 cut(s) 229, 371
SacI GAGCTC 1 cut(s) 279
SaqAI TTAA 3 cut(s) 77, 386, 588
SatI GCNGC 1 cut(s) 297
Sau3AI GATC 3 cut(s) 54, 263, 592
Sau96I GGNCC 2 cut(s) 256, 257
SchI GAGTC 1 cut(s) 225
ScrFI CCNGG 1 cut(s) 261
SduI GDGCHC 3 cut(s) 260, 279, 412
SfaNI GCATC 4 cut(s) 238, 433, 554, 566
SfcI CTRYAG 1 cut(s) 398
SmlI CTYRAG 1 cut(s) 551
SmoI CTYRAG 1 cut(s) 551
Sse9I AATT 7 cut(s) 70, 86, 155, 316, 383, 471, 498
SsiI CCGC 1 cut(s) 141
SspI AATATT 1 cut(s) 352
SspMI CTAG 1 cut(s) 300
SstI GAGCTC 1 cut(s) 279
StyD4I CCNGG 1 cut(s) 259
StyI CCWWGG 1 cut(s) 632
TaaI ACNGT 2 cut(s) 233, 651
TaiI ACGT 1 cut(s) 111
TasI AATT 7 cut(s) 70, 86, 155, 316, 383, 471, 498
TatI WGTACW 2 cut(s) 228, 370
TfiI GAWTC 2 cut(s) 464, 491
Tru1I TTAA 3 cut(s) 77, 386, 588
Tru9I TTAA 3 cut(s) 77, 386, 588
TscAI CASTG 2 cut(s) 231, 348
TseFI GTSAC 1 cut(s) 32
TseI GCWGC 1 cut(s) 296
Tsp45I GTSAC 1 cut(s) 32
TspDTI ATGAA 3 cut(s) 51, 381, 468
TspRI CASTG 2 cut(s) 231, 348
XapI RAATTY 2 cut(s) 155, 316
XspI CTAG 1 cut(s) 300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.