AT3G06460

GNS1 SUR4 membrane family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0013056
Physical Location & Seq
Forward (+)
1982659 .. 1983767
1109 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G06460.1

Sequence Viewer

Length: 897 bp
ATGGCATCTGTTTACTCCACCCTAACCTACTGGCTCGTCCACCACCCCTACATTGCCAACTTCACGTGGACCGAAGGTGAAACACTAGGCTCCACCGTTTTCTTTGTCTTTGTCGTCGTCTCCCTTTACCTCTCCGCCACATTCCTCCTCCGATACACCGTCGATTCACTCCCCACACTCGGTCCCCGCATTCTCAAACCAATCACAGCCGTTCACAGCCTCATTCTCTTCCTCCTCTCCTTAACCATGGCCGTTGGTTGCACTCTCTCCCTAATCTCTTCCTCGGACCCGAAGGCGCGTCTCTTCGACGCCGTTTGTTTCCCCCTCGACGTGAAACCTAAGGGACCGCTTTTCTTTTGGGCTCAAGTCTTTTACCTCTCGAAGATCCTTGAGTTCGTAGACACACTTCTCATCATACTCAACAAATCAATCCAACGGCTCTCGTTCCTCCACGTCTACCACCACGCAACGGTTGTGATTTTGTGCTACCTCTGGTTACGAACACGTCAATCGATGTTTCCTGTTGGGCTCGTGTTGAACTCGACGGTCCATGTGATTATGTACGGGTACTATTTCCTCTGCGCTATCGGATCGAGGCCCAAGTGGAAGAAGTTGGTGACGAATTTTCAAATGGTTCAGTTTGCTTTCGGCATGGGGTTAGGAGCCGCTTGGATGCTCCCAGAGCATTATTTCGGGTCGGGTTGCGCCGGGATTTGGACAGTTTATTTCAATGGTGTGTTTACTGCTTCTCTATTGGCTCTCTTCTACAACTTCCACTCCAAGAACTATGAGAAGACTACAACGTCGCCTTTGTATAAGATCGAATCCTTTATATTTATTCACGGAGAGAGGTGGGCAAATAAAGCGATTACATTATTTTCCAAGAAAAACGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00062 ko01110 map00062 map01110
Pfam Domains
Protein Families
ELO

Protein Analysis

298

Amino Acids

34.0

Weight (kDa)

9.34

Isoelectric Point (pI)

27.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ELO PF01151 29 - 268 3.1e-61 GNS1/SUR4 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 802
AccI GTMKAC 2 cut(s) 399, 456
AccII CGCG 1 cut(s) 298
AciI CCGC 4 cut(s) 135, 187, 347, 666
AclWI GGATC 2 cut(s) 379, 598
AcoI YGGCCR 1 cut(s) 249
AcsI RAATTY 1 cut(s) 622
AcvI CACGTG 1 cut(s) 66
AcyI GRCGYC 1 cut(s) 309
AfaI GTAC 2 cut(s) 563, 569
AfiI CCNNNNNNNGG 3 cut(s) 179, 469, 714
AflIII ACRYGT 1 cut(s) 503
AgsI TTSAA 3 cut(s) 538, 629, 730
AjiI CACGTC 3 cut(s) 331, 454, 506
Alw26I GTCTC 2 cut(s) 124, 305
AlwI GGATC 2 cut(s) 379, 598
AoxI GGCC 2 cut(s) 249, 596
ApoI RAATTY 1 cut(s) 622
AspLEI GCGC 3 cut(s) 298, 584, 707
AspS9I GGNCC 6 cut(s) 69, 182, 286, 344, 547, 597
AsuC2I CCSGG 1 cut(s) 709
AsuHPI GGTGA 2 cut(s) 89, 628
AvaII GGWCC 5 cut(s) 69, 182, 286, 344, 547
AxyI CCTNAGG 1 cut(s) 339
BanII GRGCYC 2 cut(s) 364, 531
BarI GAAGNNNNNNTAC 2 cut(s) 390, 422
BauI CACGAG 1 cut(s) 530
BbrPI CACGTG 1 cut(s) 66
BbsI GAAGAC 1 cut(s) 800
BceAI ACGGC 4 cut(s) 194, 236, 296, 452
BcnI CCSGG 1 cut(s) 709
BcoDI GTCTC 2 cut(s) 124, 305
BfaI CTAG 1 cut(s) 86
BisI GCNGC 1 cut(s) 666
BlsI GCNGC 1 cut(s) 667
Bme1390I CCNGG 1 cut(s) 709
Bme18I GGWCC 5 cut(s) 69, 182, 286, 344, 547
BmgBI CACGTC 3 cut(s) 331, 454, 506
BmgT120I GGNCC 6 cut(s) 69, 182, 286, 344, 547, 597
BmiI GGNNCC 5 cut(s) 91, 184, 288, 345, 664
BmrFI CCNGG 1 cut(s) 709
BmsI GCATC 2 cut(s) 14, 663
BpiI GAAGAC 1 cut(s) 800
BpuEI CTTGAG 2 cut(s) 348, 410
BpuMI CCSGG 1 cut(s) 709
Bsa29I ATCGAT 1 cut(s) 512
BsaAI YACGTR 1 cut(s) 66
BsaHI GRCGYC 1 cut(s) 309
BsaJI CCNNGG 2 cut(s) 246, 282
BsaXI ACNNNNNCTCC 2 cut(s) 761, 791
Bsc4I CCNNNNNNNGG 3 cut(s) 179, 469, 714
Bse1I ACTGG 1 cut(s) 35
Bse21I CCTNAGG 1 cut(s) 339
Bse3DI GCAATG 1 cut(s) 51
BseCI ATCGAT 1 cut(s) 512
BseDI CCNNGG 2 cut(s) 246, 282
BseGI GGATG 1 cut(s) 678
BseLI CCNNNNNNNGG 3 cut(s) 179, 469, 714
BseMI GCAATG 1 cut(s) 51
BseNI ACTGG 1 cut(s) 35
BseRI GAGGAG 2 cut(s) 137, 224
Bsh1236I CGCG 1 cut(s) 298
BshFI GGCC 2 cut(s) 251, 598
BshVI ATCGAT 1 cut(s) 512
BsiSI CCGG 1 cut(s) 708
BslFI GGGAC 2 cut(s) 168, 357
BslI CCNNNNNNNGG 3 cut(s) 179, 469, 714
BsmAI GTCTC 2 cut(s) 124, 305
BsmBI CGTCTC 2 cut(s) 124, 305
BsmFI GGGAC 2 cut(s) 168, 357
BsmI GAATGC 1 cut(s) 189
BsnI GGCC 2 cut(s) 251, 598
Bsp1286I GDGCHC 2 cut(s) 364, 531
Bsp143I GATC 3 cut(s) 384, 590, 819
Bsp19I CCATGG 1 cut(s) 246
BspACI CCGC 4 cut(s) 135, 187, 347, 666
BspANI GGCC 2 cut(s) 251, 598
BspDI ATCGAT 1 cut(s) 512
BspFNI CGCG 1 cut(s) 298
BspLI GGNNCC 5 cut(s) 91, 184, 288, 345, 664
BspPI GGATC 2 cut(s) 379, 598
BsrDI GCAATG 1 cut(s) 51
BsrI ACTGG 1 cut(s) 35
BssECI CCNNGG 2 cut(s) 246, 282
BssMI GATC 3 cut(s) 384, 590, 819
BssNI GRCGYC 1 cut(s) 309
BssSI CACGAG 1 cut(s) 530
BssT1I CCWWGG 1 cut(s) 246
Bst2BI CACGAG 1 cut(s) 530
Bst4CI ACNGT 5 cut(s) 97, 160, 472, 547, 721
Bst6I CTCTTC 4 cut(s) 233, 283, 308, 767
BstACI GRCGYC 1 cut(s) 309
BstBAI YACGTR 1 cut(s) 66
BstDEI CTNAG 1 cut(s) 339
BstDSI CCRYGG 1 cut(s) 246
BstF5I GGATG 1 cut(s) 678
BstFNI CGCG 1 cut(s) 298
BstHHI GCGC 3 cut(s) 298, 584, 707
BstKTI GATC 3 cut(s) 387, 593, 822
BstMAI GTCTC 2 cut(s) 124, 305
BstMBI GATC 3 cut(s) 384, 590, 819
BstMWI GCNNNNNNNGC 2 cut(s) 682, 863
BstSCI CCNGG 1 cut(s) 707
BstUI CGCG 1 cut(s) 298
BstV2I GAAGAC 1 cut(s) 800
BstX2I RGATCY 1 cut(s) 384
BstYI RGATCY 1 cut(s) 384
Bsu15I ATCGAT 1 cut(s) 512
Bsu36I CCTNAGG 1 cut(s) 339
BsuRI GGCC 2 cut(s) 251, 598
BsuTUI ATCGAT 1 cut(s) 512
BtgI CCRYGG 1 cut(s) 246
BtrI CACGTC 3 cut(s) 331, 454, 506
BtsCI GGATG 1 cut(s) 678
CfoI GCGC 3 cut(s) 298, 584, 707
Cfr13I GGNCC 6 cut(s) 69, 182, 286, 344, 547, 597
ClaI ATCGAT 1 cut(s) 512
CseI GACGC 2 cut(s) 287, 317
Csp6I GTAC 2 cut(s) 562, 568
CviAII CATG 3 cut(s) 247, 551, 652
CviQI GTAC 2 cut(s) 562, 568
DdeI CTNAG 1 cut(s) 339
DpnI GATC 3 cut(s) 386, 592, 821
DpnII GATC 3 cut(s) 384, 590, 819
DrdI GACNNNNNNGTC 1 cut(s) 802
DseDI GACNNNNNNGTC 1 cut(s) 802
EaeI YGGCCR 1 cut(s) 249
Eam1104I CTCTTC 4 cut(s) 233, 283, 308, 767
EarI CTCTTC 4 cut(s) 233, 283, 308, 767
EciI GGCGGA 1 cut(s) 124
Eco130I CCWWGG 1 cut(s) 246
Eco24I GRGCYC 2 cut(s) 364, 531
Eco47I GGWCC 5 cut(s) 69, 182, 286, 344, 547
Eco72I CACGTG 1 cut(s) 66
Eco81I CCTNAGG 1 cut(s) 339
EcoT14I CCWWGG 1 cut(s) 246
EcoT38I GRGCYC 2 cut(s) 364, 531
ErhI CCWWGG 1 cut(s) 246
Esp3I CGTCTC 2 cut(s) 124, 305
FaeI CATG 3 cut(s) 250, 554, 655
FaiI YATR 8 cut(s) 248, 416, 552, 560, 653, 789, 816, 833
FaqI GGGAC 2 cut(s) 168, 357
FatI CATG 3 cut(s) 246, 550, 651
FauI CCCGC 1 cut(s) 194
FblI GTMKAC 2 cut(s) 399, 456
Fnu4HI GCNGC 1 cut(s) 666
FokI GGATG 1 cut(s) 685
FriOI GRGCYC 2 cut(s) 364, 531
Fsp4HI GCNGC 1 cut(s) 666
FspBI CTAG 1 cut(s) 86
GlaI GCGC 3 cut(s) 297, 583, 706
GluI GCNGC 1 cut(s) 666
HaeIII GGCC 2 cut(s) 251, 598
HapII CCGG 1 cut(s) 708
HgaI GACGC 2 cut(s) 287, 317
HhaI GCGC 3 cut(s) 298, 584, 707
Hin1I GRCGYC 1 cut(s) 309
Hin1II CATG 3 cut(s) 250, 554, 655
Hin6I GCGC 3 cut(s) 296, 582, 705
HinP1I GCGC 3 cut(s) 296, 582, 705
HinfI GANTC 2 cut(s) 164, 824
HpaII CCGG 1 cut(s) 708
HphI GGTGA 2 cut(s) 89, 628
Hpy166II GTNNAC 7 cut(s) 13, 40, 69, 214, 400, 457, 741
Hpy188I TCNGA 3 cut(s) 152, 286, 590
Hpy188III TCNNGA 1 cut(s) 379
Hpy8I GTNNAC 7 cut(s) 13, 40, 69, 214, 400, 457, 741
Hpy99I CGWCG 6 cut(s) 119, 164, 311, 332, 547, 808
HpyAV CCTTC 2 cut(s) 68, 286
HpyCH4III ACNGT 5 cut(s) 97, 160, 472, 547, 721
HpyCH4IV ACGT 5 cut(s) 65, 330, 453, 505, 803
HpyCH4V TGCA 1 cut(s) 261
HpyF10VI GCNNNNNNNGC 2 cut(s) 682, 863
HpyF3I CTNAG 1 cut(s) 339
HpySE526I ACGT 5 cut(s) 65, 330, 453, 505, 803
Hsp92I GRCGYC 1 cut(s) 309
Hsp92II CATG 3 cut(s) 250, 554, 655
HspAI GCGC 3 cut(s) 296, 582, 705
Kzo9I GATC 3 cut(s) 384, 590, 819
LmnI GCTCC 3 cut(s) 95, 662, 681
LpnPI CCDG 5 cut(s) 16, 478, 534, 693, 721
LweI GCATC 2 cut(s) 14, 663
MaeI CTAG 1 cut(s) 86
MaeII ACGT 5 cut(s) 65, 330, 453, 505, 803
MaeIII GTNAC 2 cut(s) 495, 616
MalI GATC 3 cut(s) 386, 592, 821
MboI GATC 3 cut(s) 384, 590, 819
MboII GAAGA 7 cut(s) 220, 270, 295, 394, 619, 754, 805
MflI RGATCY 1 cut(s) 384
MhlI GDGCHC 2 cut(s) 364, 531
MluCI AATT 1 cut(s) 622
MmeI TCCRAC 1 cut(s) 457
MseI TTAA 2 cut(s) 242, 895
MspI CCGG 1 cut(s) 708
MspR9I CCNGG 1 cut(s) 709
Mva1269I GAATGC 1 cut(s) 189
MvnI CGCG 1 cut(s) 298
MwoI GCNNNNNNNGC 2 cut(s) 682, 863
NciI CCSGG 1 cut(s) 709
NcoI CCATGG 1 cut(s) 246
NdeII GATC 3 cut(s) 384, 590, 819
NlaIII CATG 3 cut(s) 250, 554, 655
NlaIV GGNNCC 5 cut(s) 91, 184, 288, 345, 664
NmuCI GTSAC 1 cut(s) 616
PctI GAATGC 1 cut(s) 189
PfeI GAWTC 2 cut(s) 164, 824
PkrI GCNGC 1 cut(s) 667
PmaCI CACGTG 1 cut(s) 66
PmlI CACGTG 1 cut(s) 66
Ppu21I YACGTR 1 cut(s) 66
PspCI CACGTG 1 cut(s) 66
PspN4I GGNNCC 5 cut(s) 91, 184, 288, 345, 664
PspPI GGNCC 6 cut(s) 69, 182, 286, 344, 547, 597
PsuI RGATCY 1 cut(s) 384
RsaI GTAC 2 cut(s) 563, 569
RsaNI GTAC 2 cut(s) 562, 568
SaqAI TTAA 2 cut(s) 242, 895
SatI GCNGC 1 cut(s) 666
Sau3AI GATC 3 cut(s) 384, 590, 819
Sau96I GGNCC 6 cut(s) 69, 182, 286, 344, 547, 597
ScrFI CCNGG 1 cut(s) 709
SduI GDGCHC 2 cut(s) 364, 531
SfaNI GCATC 2 cut(s) 14, 663
SinI GGWCC 5 cut(s) 69, 182, 286, 344, 547
SmlI CTYRAG 2 cut(s) 363, 389
SmoI CTYRAG 2 cut(s) 363, 389
Sse9I AATT 1 cut(s) 622
SsiI CCGC 4 cut(s) 135, 187, 347, 666
SspMI CTAG 1 cut(s) 86
StyD4I CCNGG 1 cut(s) 707
StyI CCWWGG 1 cut(s) 246
TaaI ACNGT 5 cut(s) 97, 160, 472, 547, 721
TaiI ACGT 5 cut(s) 68, 333, 456, 508, 806
TaqI TCGA 8 cut(s) 162, 306, 327, 380, 512, 542, 593, 822
TaqII GACCGA 2 cut(s) 86, 170
TasI AATT 1 cut(s) 622
TauI GCSGC 1 cut(s) 668
TfiI GAWTC 2 cut(s) 164, 824
Tru1I TTAA 2 cut(s) 242, 895
Tru9I TTAA 2 cut(s) 242, 895
TseFI GTSAC 1 cut(s) 616
Tsp45I GTSAC 1 cut(s) 616
TspGWI ACGGA 1 cut(s) 858
VpaK11BI GGWCC 5 cut(s) 69, 182, 286, 344, 547
XapI RAATTY 1 cut(s) 622
XmiI GTMKAC 2 cut(s) 399, 456
XspI CTAG 1 cut(s) 86
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.