AT3G06470

GNS1 SUR4 membrane family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0013056
Physical Location & Seq
Forward (+)
1983731 .. 1985330
1600 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G06470.1

Sequence Viewer

Length: 837 bp
ATGGCATCAATTTACTCCTCTTTAACCTACTGGCTCGTTAACCACCCCTACATCTCCAATTTTACTTGGATCGAAGGTGAAACCCTAGGCTCCACCGTCTTTTTCGTATCCGTCGTAGTCTCCGTTTACCTCTCCGCCACGTTCCTCCTCCGATCCGCCATCGATTCACTCCCATCACTCAGTCCACGTATCCTCAAACCGATCACAGCCGTCCACAGCCTAATCCTCTGTCTCCTCTCCTTAGTCATGGCCGTCGGTTGCACTCTCTCAATAACCTCATCTCACGCGTCTTCAGATCCGATGGCGCGTTTCCTTCACGCGATTTGCTTTCCCGTCGACGTTAAACCTAACGGACCGCTTTTCTTCTGGGCTCAAGTCTTCTACCTCTCGAAGATCCTCGAGTTCGGAGACACGATCCTCATCATACTCGGCAAATCAATCCAACGGCTATCCTTCCTCCACGTGTACCACCACGCGACGGTTGTGGTCATGTGTTATCTCTGGCTCCGAACTCGCCAATCGATGTTTCCGATTGCGCTCGTGACGAATTCGACGGTACACGTCATCATGTACGGTTACTACTTCCTCTGCGCCGTTGGATCGAGGCCCAAGTGGAAGAGATTGGTGACGGATTGTCAGATTGTTCAGTTTGTTTTCAGTTTCGGGTTATCCGGTTGGATGCTCCGAGAGCACTTATTCGGGTCGGGTTGCACCGGGATTTGGGGATGGTGTTTCAACGCTGCATTTAATGCTTCTCTTTTGGCTCTCTTTTCCAACTTCCATTCAAAGAATTATGTCAAGAAGCCAACGAGAGAGGATGGCAAAAAAAGCGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ELO

Protein Analysis

278

Amino Acids

31.31

Weight (kDa)

9.21

Isoelectric Point (pI)

30.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ELO PF01151 29 - 271 1.8e-63 GNS1/SUR4 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 336
AccII CGCG 4 cut(s) 287, 307, 320, 476
AciI CCGC 3 cut(s) 135, 156, 356
AclWI GGATC 6 cut(s) 77, 147, 290, 388, 409, 607
AcoI YGGCCR 1 cut(s) 249
AcsI RAATTY 1 cut(s) 547
AcuI CTGAAG 1 cut(s) 276
AcvI CACGTG 1 cut(s) 463
AfaI GTAC 3 cut(s) 467, 558, 572
AfiI CCNNNNNNNGG 2 cut(s) 478, 720
AflIII ACRYGT 3 cut(s) 285, 462, 559
AgsI TTSAA 2 cut(s) 736, 786
AhdI GACNNNNNGTC 1 cut(s) 633
AjiI CACGTC 1 cut(s) 562
AjuI GAANNNNNNNTTGG 2 cut(s) 510, 542
Alw21I GWGCWC 1 cut(s) 693
Alw26I GTCTC 3 cut(s) 124, 236, 402
AlwI GGATC 6 cut(s) 77, 147, 290, 388, 409, 607
Ama87I CYCGRG 1 cut(s) 398
AoxI GGCC 2 cut(s) 249, 605
ApeKI GCWGC 1 cut(s) 740
ApoI RAATTY 1 cut(s) 547
AspA2I CCTAGG 1 cut(s) 85
AspLEI GCGC 3 cut(s) 307, 538, 593
AspS9I GGNCC 2 cut(s) 353, 606
AsuC2I CCSGG 1 cut(s) 715
AsuHPI GGTGA 2 cut(s) 89, 637
AvaI CYCGRG 1 cut(s) 398
AvaII GGWCC 1 cut(s) 353
AvrII CCTAGG 1 cut(s) 85
BanII GRGCYC 1 cut(s) 373
BauI CACGAG 1 cut(s) 539
BbrPI CACGTG 1 cut(s) 463
BbsI GAAGAC 2 cut(s) 282, 370
Bbv12I GWGCWC 1 cut(s) 693
BbvI GCAGC 1 cut(s) 727
BccI CCATC 5 cut(s) 167, 181, 295, 720, 812
BceAI ACGGC 4 cut(s) 194, 236, 461, 578
BciVI GTATCC 2 cut(s) 118, 200
BcnI CCSGG 1 cut(s) 715
BcoDI GTCTC 3 cut(s) 124, 236, 402
BfaI CTAG 1 cut(s) 86
BfuI GTATCC 2 cut(s) 118, 200
BisI GCNGC 1 cut(s) 741
BlnI CCTAGG 1 cut(s) 85
BlsI GCNGC 1 cut(s) 742
Bme1390I CCNGG 1 cut(s) 715
Bme18I GGWCC 1 cut(s) 353
BmeRI GACNNNNNGTC 1 cut(s) 633
BmeT110I CYCGRG 1 cut(s) 398
BmgBI CACGTC 1 cut(s) 562
BmgT120I GGNCC 2 cut(s) 353, 606
BmiI GGNNCC 2 cut(s) 91, 506
BmrFI CCNGG 1 cut(s) 715
BmsI GCATC 2 cut(s) 14, 669
BpiI GAAGAC 2 cut(s) 282, 370
BpuEI CTTGAG 1 cut(s) 357
BpuMI CCSGG 1 cut(s) 715
Bsa29I ATCGAT 2 cut(s) 162, 521
BsaAI YACGTR 2 cut(s) 188, 463
BsaBI GATNNNNATC 1 cut(s) 419
BsaJI CCNNGG 1 cut(s) 85
BsaWI WCCGGW 1 cut(s) 671
Bsc4I CCNNNNNNNGG 2 cut(s) 478, 720
Bse1I ACTGG 1 cut(s) 35
Bse8I GATNNNNATC 1 cut(s) 419
BseCI ATCGAT 2 cut(s) 162, 521
BseDI CCNNGG 1 cut(s) 85
BseGI GGATG 3 cut(s) 684, 731, 823
BseJI GATNNNNATC 1 cut(s) 419
BseLI CCNNNNNNNGG 2 cut(s) 478, 720
BseMII CTCAG 1 cut(s) 193
BseNI ACTGG 1 cut(s) 35
BseRI GAGGAG 3 cut(s) 7, 137, 224
BseXI GCAGC 1 cut(s) 727
Bsh1236I CGCG 4 cut(s) 287, 307, 320, 476
BshFI GGCC 2 cut(s) 251, 607
BshVI ATCGAT 2 cut(s) 162, 521
BsiHKAI GWGCWC 1 cut(s) 693
BsiHKCI CYCGRG 1 cut(s) 398
BsiSI CCGG 2 cut(s) 672, 714
BslI CCNNNNNNNGG 2 cut(s) 478, 720
BsmAI GTCTC 3 cut(s) 124, 236, 402
BsnI GGCC 2 cut(s) 251, 607
BsoBI CYCGRG 1 cut(s) 398
Bsp1286I GDGCHC 2 cut(s) 373, 693
Bsp143I GATC 7 cut(s) 69, 152, 201, 295, 393, 414, 599
BspACI CCGC 3 cut(s) 135, 156, 356
BspANI GGCC 2 cut(s) 251, 607
BspCNI CTCAG 1 cut(s) 192
BspDI ATCGAT 2 cut(s) 162, 521
BspFNI CGCG 4 cut(s) 287, 307, 320, 476
BspLI GGNNCC 2 cut(s) 91, 506
BspPI GGATC 6 cut(s) 77, 147, 290, 388, 409, 607
BsrI ACTGG 1 cut(s) 35
BssECI CCNNGG 1 cut(s) 85
BssMI GATC 7 cut(s) 69, 152, 201, 295, 393, 414, 599
BssSI CACGAG 1 cut(s) 539
BssT1I CCWWGG 1 cut(s) 85
Bst2BI CACGAG 1 cut(s) 539
Bst4CI ACNGT 4 cut(s) 97, 481, 556, 575
Bst6I CTCTTC 1 cut(s) 611
BstAPI GCANNNNNTGC 1 cut(s) 749
BstBAI YACGTR 2 cut(s) 188, 463
BstDEI CTNAG 2 cut(s) 179, 241
BstF5I GGATG 3 cut(s) 684, 731, 823
BstFNI CGCG 4 cut(s) 287, 307, 320, 476
BstHHI GCGC 3 cut(s) 307, 538, 593
BstKTI GATC 7 cut(s) 72, 155, 204, 298, 396, 417, 602
BstMAI GTCTC 3 cut(s) 124, 236, 402
BstMBI GATC 7 cut(s) 69, 152, 201, 295, 393, 414, 599
BstMWI GCNNNNNNNGC 3 cut(s) 688, 749, 828
BstSCI CCNGG 1 cut(s) 713
BstUI CGCG 4 cut(s) 287, 307, 320, 476
BstV1I GCAGC 1 cut(s) 727
BstV2I GAAGAC 2 cut(s) 282, 370
BstX2I RGATCY 2 cut(s) 295, 393
BstYI RGATCY 2 cut(s) 295, 393
Bsu15I ATCGAT 2 cut(s) 162, 521
BsuI GTATCC 2 cut(s) 118, 200
BsuRI GGCC 2 cut(s) 251, 607
BsuTUI ATCGAT 2 cut(s) 162, 521
BtrI CACGTC 1 cut(s) 562
BtsCI GGATG 3 cut(s) 684, 731, 823
CfoI GCGC 3 cut(s) 307, 538, 593
Cfr13I GGNCC 2 cut(s) 353, 606
ClaI ATCGAT 2 cut(s) 162, 521
CpoI CGGWCCG 1 cut(s) 353
CseI GACGC 1 cut(s) 276
Csp6I GTAC 3 cut(s) 466, 557, 571
CspI CGGWCCG 1 cut(s) 353
CviAII CATG 3 cut(s) 247, 490, 568
CviQI GTAC 3 cut(s) 466, 557, 571
DdeI CTNAG 2 cut(s) 179, 241
DpnI GATC 7 cut(s) 71, 154, 203, 297, 395, 416, 601
DpnII GATC 7 cut(s) 69, 152, 201, 295, 393, 414, 599
DriI GACNNNNNGTC 1 cut(s) 633
EaeI YGGCCR 1 cut(s) 249
Eam1104I CTCTTC 1 cut(s) 611
Eam1105I GACNNNNNGTC 1 cut(s) 633
EarI CTCTTC 1 cut(s) 611
EciI GGCGGA 2 cut(s) 124, 145
Eco130I CCWWGG 1 cut(s) 85
Eco24I GRGCYC 1 cut(s) 373
Eco47I GGWCC 1 cut(s) 353
Eco57I CTGAAG 1 cut(s) 276
Eco72I CACGTG 1 cut(s) 463
Eco88I CYCGRG 1 cut(s) 398
EcoRI GAATTC 1 cut(s) 547
EcoT14I CCWWGG 1 cut(s) 85
EcoT38I GRGCYC 1 cut(s) 373
ErhI CCWWGG 1 cut(s) 85
FaeI CATG 3 cut(s) 250, 493, 571
FaiI YATR 5 cut(s) 248, 425, 491, 569, 795
FatI CATG 3 cut(s) 246, 489, 567
FblI GTMKAC 1 cut(s) 336
Fnu4HI GCNGC 1 cut(s) 741
FokI GGATG 3 cut(s) 691, 738, 830
FriOI GRGCYC 1 cut(s) 373
Fsp4HI GCNGC 1 cut(s) 741
FspBI CTAG 1 cut(s) 86
GlaI GCGC 3 cut(s) 306, 537, 592
GluI GCNGC 1 cut(s) 741
HaeIII GGCC 2 cut(s) 251, 607
HapII CCGG 2 cut(s) 672, 714
HgaI GACGC 1 cut(s) 276
HhaI GCGC 3 cut(s) 307, 538, 593
Hin1II CATG 3 cut(s) 250, 493, 571
Hin6I GCGC 3 cut(s) 305, 536, 591
HinP1I GCGC 3 cut(s) 305, 536, 591
HincII GTYRAC 2 cut(s) 40, 337
HindII GTYRAC 2 cut(s) 40, 337
HinfI GANTC 1 cut(s) 164
HpaI GTTAAC 1 cut(s) 40
HpaII CCGG 2 cut(s) 672, 714
HphI GGTGA 2 cut(s) 89, 637
Hpy166II GTNNAC 7 cut(s) 40, 127, 185, 214, 337, 466, 559
Hpy188I TCNGA 8 cut(s) 152, 295, 300, 407, 509, 531, 639, 686
Hpy188III TCNNGA 3 cut(s) 388, 541, 799
Hpy8I GTNNAC 7 cut(s) 40, 127, 185, 214, 337, 466, 559
Hpy99I CGWCG 6 cut(s) 116, 257, 338, 341, 481, 556
HpyAV CCTTC 3 cut(s) 68, 323, 463
HpyCH4III ACNGT 4 cut(s) 97, 481, 556, 575
HpyCH4IV ACGT 5 cut(s) 140, 187, 339, 462, 561
HpyCH4V TGCA 3 cut(s) 261, 711, 743
HpyF10VI GCNNNNNNNGC 3 cut(s) 688, 749, 828
HpyF3I CTNAG 2 cut(s) 179, 241
HpySE526I ACGT 5 cut(s) 140, 187, 339, 462, 561
Hsp92II CATG 3 cut(s) 250, 493, 571
HspAI GCGC 3 cut(s) 305, 536, 591
KspAI GTTAAC 1 cut(s) 40
Kzo9I GATC 7 cut(s) 69, 152, 201, 295, 393, 414, 599
LmnI GCTCC 3 cut(s) 95, 510, 687
LpnPI CCDG 5 cut(s) 16, 352, 487, 685, 727
Lsp1109I GCAGC 1 cut(s) 727
LweI GCATC 2 cut(s) 14, 669
MaeI CTAG 1 cut(s) 86
MaeII ACGT 5 cut(s) 140, 187, 339, 462, 561
MaeIII GTNAC 3 cut(s) 541, 575, 625
MalI GATC 7 cut(s) 71, 154, 203, 297, 395, 416, 601
MboI GATC 7 cut(s) 69, 152, 201, 295, 393, 414, 599
MboII GAAGA 5 cut(s) 282, 355, 370, 403, 628
MflI RGATCY 2 cut(s) 295, 393
MhlI GDGCHC 2 cut(s) 373, 693
MluCI AATT 4 cut(s) 9, 58, 547, 790
MluI ACGCGT 1 cut(s) 285
MmeI TCCRAC 4 cut(s) 466, 577, 656, 798
MseI TTAA 4 cut(s) 23, 39, 342, 747
MspI CCGG 2 cut(s) 672, 714
MspR9I CCNGG 1 cut(s) 715
MvnI CGCG 4 cut(s) 287, 307, 320, 476
MwoI GCNNNNNNNGC 3 cut(s) 688, 749, 828
NciI CCSGG 1 cut(s) 715
NdeII GATC 7 cut(s) 69, 152, 201, 295, 393, 414, 599
NlaIII CATG 3 cut(s) 250, 493, 571
NlaIV GGNNCC 2 cut(s) 91, 506
NmeAIII GCCGAG 1 cut(s) 408
NmuCI GTSAC 2 cut(s) 541, 625
PaeR7I CTCGAG 1 cut(s) 398
PcsI WCGNNNNNNNCGW 3 cut(s) 111, 120, 527
PfeI GAWTC 1 cut(s) 164
PkrI GCNGC 1 cut(s) 742
PmaCI CACGTG 1 cut(s) 463
PmlI CACGTG 1 cut(s) 463
Ppu21I YACGTR 2 cut(s) 188, 463
PspCI CACGTG 1 cut(s) 463
PspN4I GGNNCC 2 cut(s) 91, 506
PspPI GGNCC 2 cut(s) 353, 606
PspXI VCTCGAGB 1 cut(s) 398
PsuI RGATCY 2 cut(s) 295, 393
RsaI GTAC 3 cut(s) 467, 558, 572
RsaNI GTAC 3 cut(s) 466, 557, 571
Rsr2I CGGWCCG 1 cut(s) 353
RsrII CGGWCCG 1 cut(s) 353
SalI GTCGAC 1 cut(s) 335
SaqAI TTAA 4 cut(s) 23, 39, 342, 747
SatI GCNGC 1 cut(s) 741
Sau3AI GATC 7 cut(s) 69, 152, 201, 295, 393, 414, 599
Sau96I GGNCC 2 cut(s) 353, 606
ScrFI CCNGG 1 cut(s) 715
SduI GDGCHC 2 cut(s) 373, 693
SfaNI GCATC 2 cut(s) 14, 669
Sfr274I CTCGAG 1 cut(s) 398
SgrDI CGTCGACG 1 cut(s) 335
SinI GGWCC 1 cut(s) 353
SlaI CTCGAG 1 cut(s) 398
SmlI CTYRAG 2 cut(s) 372, 398
SmoI CTYRAG 2 cut(s) 372, 398
Sse9I AATT 4 cut(s) 9, 58, 547, 790
SsiI CCGC 3 cut(s) 135, 156, 356
SspMI CTAG 1 cut(s) 86
StyD4I CCNGG 1 cut(s) 713
StyI CCWWGG 1 cut(s) 85
TaaI ACNGT 4 cut(s) 97, 481, 556, 575
TaiI ACGT 5 cut(s) 143, 190, 342, 465, 564
TaqI TCGA 8 cut(s) 72, 162, 336, 389, 399, 521, 551, 602
TasI AATT 4 cut(s) 9, 58, 547, 790
TfiI GAWTC 1 cut(s) 164
Tru1I TTAA 4 cut(s) 23, 39, 342, 747
Tru9I TTAA 4 cut(s) 23, 39, 342, 747
TseFI GTSAC 2 cut(s) 541, 625
TseI GCWGC 1 cut(s) 740
Tsp45I GTSAC 2 cut(s) 541, 625
TspGWI ACGGA 4 cut(s) 100, 112, 366, 644
VpaK11BI GGWCC 1 cut(s) 353
XapI RAATTY 1 cut(s) 547
XhoI CTCGAG 1 cut(s) 398
XmaJI CCTAGG 1 cut(s) 85
XmiI GTMKAC 1 cut(s) 336
XspI CTAG 1 cut(s) 86
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.