AT3G07720

Nitrile-specifier protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0005031
Physical Location & Seq
Forward (+)
2465235 .. 2467270
2036 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G07720.1

Sequence Viewer

Length: 990 bp
ATGGCAGCGACTCCAATGGAAGGCAAATGGGTTCAGCTTAAACAGAAAGGAACCGGACCAGGAGCAAGAAGCTCTCATGCTATAGCTCTTGTGGGAAACAAAATGTATGCCTTTGGTGGCGAGTTTCAACCACGTGTTCCTGTTGACAACCAGCTCTACGTTTTCGACCTCGAAACTCAGACATGGTCTATCCAGGAAGCTTCTGGGGATGCTCCTCCTCCTAGAGTAGGTGTAGCCATGGCTGCAGTCGGACCCATCATCTATTTCTTTGGTGGCCGGGACTCGACTCACCAGGAGCTCAACGAGTTATACTGTTTTAACACTTTGACCAACCAGTGGAAACTACTCTCTTCAGGGGAAACCGGTCCACAGAACCGAAGCTACCACTCTATTACTGCGGATTCTCAGAACGTGTATGTGTTTGGTGGATGCGGAGTTGATGGGCGTCTCAACGATCTATGGGCTTACAATGTCGTTGATCAGAAATGGATTAAGTTCCCATCTCCGGGAGAAGCTTGTAGAGGAAGAGGCGGTCCAGGACTCGAGGTGGTTCAAGGAAAGATCTGGGTGGTATATGGATTCGCAGGAGAAGAAGCAGACGATGTCCACTGTTTCGACATAGCTAAAGGGGAATGGAAAGAAGTTGAGACAAAAGGCGAAAAACCGTCAGCAAGAAGTGTGTTTTCGACTGCGGTTGTTGGGAAACAGATACTGATATCTGGAGGAGAGATTGACCCGAGCGATCTGGGGCATATGGGAGCAGGATGTTTCACAGGTGACGCTTATGGGCTTGATACAGAGACGCTGGAATGGAGGAAATGGGAGGATGGGGTTGGATCGGTGGAGCATCCCGGTCCTAGAGGATGGTGCGCGTTTGCAGCTGGATCACGAGATGGTAAGCAAGGTCTGTTGGTTTATGGCGGGAACTCGCCGAGCAATGATAGGCTTGATGATATCTTCTTCTTCACTCCCAACACTCTACTCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kelch_1 Kelch_3 Kelch_4

Protein Analysis

329

Amino Acids

35.69

Weight (kDa)

5.07

Isoelectric Point (pI)

25.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_FBX42 PF13415 7 - 100 8.3e-17 FBX42, beta-propeller domain
Beta-prop_ATRN-LZTR1 PF24981 8 - 81 2.2e-10 Attractin/LZTR1 beta-propeller
Kelch_1 PF01344 22 - 64 1.1e-06 Kelch motif
Kelch_KLHDC2_KLHL20_DRC7 PF24681 25 - 242 7.8e-71 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_HCF PF13854 63 - 165 3.1e-07 Host cell factor, Kelch-repeats domain
Kelch_FKB95 PF25210 72 - 174 8.8e-06 FKB95, Kelch-repeats domain
Kelch_1 PF01344 74 - 118 7.2e-10 Kelch motif
Beta-prop_FBX42 PF13415 87 - 243 6.7e-25 FBX42, beta-propeller domain
Beta-prop_ATRN-LZTR1 PF24981 87 - 168 2.1e-14 Attractin/LZTR1 beta-propeller
Kelch_1 PF01344 126 - 169 5.4e-08 Kelch motif
Kelch_FKB95 PF25210 126 - 241 1.2e-06 FKB95, Kelch-repeats domain
Beta-prop_FBX42 PF13415 135 - 323 2.1e-20 FBX42, beta-propeller domain
Kelch_1 PF01344 176 - 217 1.9e-06 Kelch motif
Kelch_HCF PF13854 184 - 247 1.2e-06 Host cell factor, Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 188 - 320 5e-10 Attractin/LZTR1 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 336
AccII CGCG 1 cut(s) 872
AciI CCGC 5 cut(s) 398, 432, 531, 692, 921
AclWI GGATC 2 cut(s) 844, 892
AcoI YGGCCR 1 cut(s) 274
AcuI CTGAAG 1 cut(s) 336
AcvI CACGTG 1 cut(s) 134
AcyI GRCGYC 1 cut(s) 445
AfiI CCNNNNNNNGG 5 cut(s) 20, 227, 336, 505, 506
AflIII ACRYGT 2 cut(s) 133, 411
AgeI ACCGGT 1 cut(s) 362
AgsI TTSAA 2 cut(s) 128, 554
AjnI CCWGG 4 cut(s) 58, 192, 291, 535
Alw21I GWGCWC 1 cut(s) 300
Alw26I GTCTC 3 cut(s) 452, 641, 794
AlwI GGATC 2 cut(s) 844, 892
AlwNI CAGNNNCTG 1 cut(s) 712
Ama87I CYCGRG 2 cut(s) 542, 736
AoxI GGCC 1 cut(s) 274
ApeKI GCWGC 3 cut(s) 5, 242, 878
AsiGI ACCGGT 1 cut(s) 362
AspLEI GCGC 1 cut(s) 872
AspS9I GGNCC 5 cut(s) 56, 251, 365, 533, 854
AsuC2I CCSGG 3 cut(s) 278, 507, 852
AsuHPI GGTGA 2 cut(s) 281, 788
AvaI CYCGRG 2 cut(s) 542, 736
AvaII GGWCC 5 cut(s) 56, 251, 365, 533, 854
BanII GRGCYC 1 cut(s) 300
BauI CACGAG 1 cut(s) 888
BbrPI CACGTG 1 cut(s) 134
Bbv12I GWGCWC 1 cut(s) 300
BbvI GCAGC 3 cut(s) 17, 229, 890
BccI CCATC 6 cut(s) 263, 434, 508, 821, 858, 887
BciT130I CCWGG 4 cut(s) 60, 194, 293, 537
BclI TGATCA 1 cut(s) 478
BcnI CCSGG 3 cut(s) 278, 507, 852
BcoDI GTCTC 3 cut(s) 452, 641, 794
BfaI CTAG 2 cut(s) 222, 858
BfmI CTRYAG 2 cut(s) 81, 243
BglII AGATCT 1 cut(s) 561
BisI GCNGC 3 cut(s) 6, 243, 879
BlsI GCNGC 3 cut(s) 7, 244, 880
Bme1390I CCNGG 7 cut(s) 60, 194, 278, 293, 507, 537, 852
Bme18I GGWCC 5 cut(s) 56, 251, 365, 533, 854
BmeT110I CYCGRG 2 cut(s) 542, 736
BmgT120I GGNCC 5 cut(s) 56, 251, 365, 533, 854
BmiI GGNNCC 2 cut(s) 52, 253
BmrFI CCNGG 7 cut(s) 60, 194, 278, 293, 507, 537, 852
BmsI GCATC 3 cut(s) 199, 419, 856
BpmI CTGGAG 1 cut(s) 741
BpuEI CTTGAG 1 cut(s) 968
BpuMI CCSGG 3 cut(s) 278, 507, 852
BsaAI YACGTR 1 cut(s) 134
BsaHI GRCGYC 1 cut(s) 445
BsaJI CCNNGG 1 cut(s) 237
BsaWI WCCGGW 2 cut(s) 53, 362
Bsc4I CCNNNNNNNGG 5 cut(s) 20, 227, 336, 505, 506
Bse118I RCCGGY 1 cut(s) 362
Bse1I ACTGG 1 cut(s) 334
Bse3DI GCAATG 1 cut(s) 943
BseBI CCWGG 4 cut(s) 60, 194, 293, 537
BseDI CCNNGG 1 cut(s) 237
BseGI GGATG 6 cut(s) 214, 434, 770, 832, 847, 869
BseLI CCNNNNNNNGG 5 cut(s) 20, 227, 336, 505, 506
BseMI GCAATG 1 cut(s) 943
BseMII CTCAG 2 cut(s) 191, 419
BseNI ACTGG 1 cut(s) 334
BseRI GAGGAG 3 cut(s) 204, 207, 738
BseXI GCAGC 3 cut(s) 17, 229, 890
Bsh1236I CGCG 1 cut(s) 872
BshFI GGCC 1 cut(s) 276
BshTI ACCGGT 1 cut(s) 362
BsiHKAI GWGCWC 1 cut(s) 300
BsiHKCI CYCGRG 2 cut(s) 542, 736
BsiSI CCGG 5 cut(s) 54, 277, 363, 506, 852
BslFI GGGAC 1 cut(s) 293
BslI CCNNNNNNNGG 5 cut(s) 20, 227, 336, 505, 506
BsmAI GTCTC 3 cut(s) 452, 641, 794
BsmBI CGTCTC 2 cut(s) 452, 794
BsmFI GGGAC 1 cut(s) 293
BsnI GGCC 1 cut(s) 276
BsoBI CYCGRG 2 cut(s) 542, 736
Bsp1286I GDGCHC 1 cut(s) 300
Bsp143I GATC 6 cut(s) 454, 478, 561, 742, 836, 884
Bsp19I CCATGG 1 cut(s) 237
BspACI CCGC 5 cut(s) 398, 432, 531, 692, 921
BspANI GGCC 1 cut(s) 276
BspCNI CTCAG 2 cut(s) 190, 418
BspFNI CGCG 1 cut(s) 872
BspLI GGNNCC 2 cut(s) 52, 253
BspMAI CTGCAG 1 cut(s) 247
BspPI GGATC 2 cut(s) 844, 892
BsrDI GCAATG 1 cut(s) 943
BsrFI RCCGGY 1 cut(s) 362
BsrI ACTGG 1 cut(s) 334
BssAI RCCGGY 1 cut(s) 362
BssECI CCNNGG 1 cut(s) 237
BssMI GATC 6 cut(s) 454, 478, 561, 742, 836, 884
BssNI GRCGYC 1 cut(s) 445
BssSI CACGAG 1 cut(s) 888
BssT1I CCWWGG 1 cut(s) 237
Bst2BI CACGAG 1 cut(s) 888
Bst2UI CCWGG 4 cut(s) 60, 194, 293, 537
Bst4CI ACNGT 3 cut(s) 314, 611, 666
Bst6I CTCTTC 2 cut(s) 355, 520
BstACI GRCGYC 1 cut(s) 445
BstBAI YACGTR 1 cut(s) 134
BstDEI CTNAG 2 cut(s) 177, 405
BstDSI CCRYGG 1 cut(s) 237
BstENI CCTNNNNNAGG 1 cut(s) 225
BstF5I GGATG 6 cut(s) 214, 434, 770, 832, 847, 869
BstFNI CGCG 1 cut(s) 872
BstHHI GCGC 1 cut(s) 872
BstKTI GATC 6 cut(s) 457, 481, 564, 745, 839, 887
BstMAI GTCTC 3 cut(s) 452, 641, 794
BstMBI GATC 6 cut(s) 454, 478, 561, 742, 836, 884
BstMWI GCNNNNNNNGC 2 cut(s) 242, 878
BstNI CCWGG 4 cut(s) 60, 194, 293, 537
BstSCI CCNGG 7 cut(s) 58, 192, 276, 291, 505, 535, 850
BstSFI CTRYAG 2 cut(s) 81, 243
BstUI CGCG 1 cut(s) 872
BstV1I GCAGC 3 cut(s) 17, 229, 890
BstX2I RGATCY 1 cut(s) 561
BstYI RGATCY 1 cut(s) 561
BsuRI GGCC 1 cut(s) 276
BtgI CCRYGG 1 cut(s) 237
BtsCI GGATG 6 cut(s) 214, 434, 770, 832, 847, 869
BtsIMutI CAGTG 2 cut(s) 341, 607
CaiI CAGNNNCTG 1 cut(s) 712
CfoI GCGC 1 cut(s) 872
Cfr10I RCCGGY 1 cut(s) 362
Cfr13I GGNCC 5 cut(s) 56, 251, 365, 533, 854
CseI GACGC 3 cut(s) 434, 788, 811
CspAI ACCGGT 1 cut(s) 362
CviAII CATG 3 cut(s) 77, 183, 238
DdeI CTNAG 2 cut(s) 177, 405
DpnI GATC 6 cut(s) 456, 480, 563, 744, 838, 886
DpnII GATC 6 cut(s) 454, 478, 561, 742, 836, 884
EaeI YGGCCR 1 cut(s) 274
Eam1104I CTCTTC 2 cut(s) 355, 520
EarI CTCTTC 2 cut(s) 355, 520
Ecl136II GAGCTC 1 cut(s) 298
Eco130I CCWWGG 1 cut(s) 237
Eco24I GRGCYC 1 cut(s) 300
Eco32I GATATC 2 cut(s) 717, 955
Eco47I GGWCC 5 cut(s) 56, 251, 365, 533, 854
Eco53kI GAGCTC 1 cut(s) 298
Eco57I CTGAAG 1 cut(s) 336
Eco72I CACGTG 1 cut(s) 134
Eco88I CYCGRG 2 cut(s) 542, 736
EcoICRI GAGCTC 1 cut(s) 298
EcoNI CCTNNNNNAGG 1 cut(s) 225
EcoRII CCWGG 4 cut(s) 58, 192, 291, 535
EcoRV GATATC 2 cut(s) 717, 955
EcoT14I CCWWGG 1 cut(s) 237
EcoT38I GRGCYC 1 cut(s) 300
ErhI CCWWGG 1 cut(s) 237
Esp3I CGTCTC 2 cut(s) 452, 794
FaeI CATG 3 cut(s) 80, 186, 241
FaqI GGGAC 1 cut(s) 293
FatI CATG 3 cut(s) 76, 182, 237
FauI CCCGC 1 cut(s) 914
FauNDI CATATG 1 cut(s) 753
FbaI TGATCA 1 cut(s) 478
Fnu4HI GCNGC 3 cut(s) 6, 243, 879
FokI GGATG 6 cut(s) 221, 441, 777, 834, 839, 876
FriOI GRGCYC 1 cut(s) 300
Fsp4HI GCNGC 3 cut(s) 6, 243, 879
FspBI CTAG 2 cut(s) 222, 858
GlaI GCGC 1 cut(s) 871
GluI GCNGC 3 cut(s) 6, 243, 879
GsuI CTGGAG 1 cut(s) 741
HaeIII GGCC 1 cut(s) 276
HapII CCGG 5 cut(s) 54, 277, 363, 506, 852
HgaI GACGC 3 cut(s) 434, 788, 811
HhaI GCGC 1 cut(s) 872
Hin1I GRCGYC 1 cut(s) 445
Hin1II CATG 3 cut(s) 80, 186, 241
Hin6I GCGC 1 cut(s) 870
HinP1I GCGC 1 cut(s) 870
HincII GTYRAC 1 cut(s) 145
HindII GTYRAC 1 cut(s) 145
HindIII AAGCTT 2 cut(s) 198, 513
HinfI GANTC 6 cut(s) 10, 281, 286, 401, 540, 579
HpaII CCGG 5 cut(s) 54, 277, 363, 506, 852
HphI GGTGA 2 cut(s) 281, 788
Hpy166II GTNNAC 3 cut(s) 145, 368, 607
Hpy188I TCNGA 4 cut(s) 180, 251, 408, 483
Hpy188III TCNNGA 2 cut(s) 720, 888
Hpy8I GTNNAC 3 cut(s) 145, 368, 607
HpyAV CCTTC 1 cut(s) 14
HpyCH4III ACNGT 3 cut(s) 314, 611, 666
HpyCH4IV ACGT 3 cut(s) 133, 159, 411
HpyCH4V TGCA 2 cut(s) 245, 878
HpyF10VI GCNNNNNNNGC 2 cut(s) 242, 878
HpyF3I CTNAG 2 cut(s) 177, 405
HpySE526I ACGT 3 cut(s) 133, 159, 411
Hsp92I GRCGYC 1 cut(s) 445
Hsp92II CATG 3 cut(s) 80, 186, 241
HspAI GCGC 1 cut(s) 870
Ksp22I TGATCA 1 cut(s) 478
Kzo9I GATC 6 cut(s) 454, 478, 561, 742, 836, 884
LmnI GCTCC 5 cut(s) 62, 217, 295, 758, 844
Lsp1109I GCAGC 3 cut(s) 17, 229, 890
LweI GCATC 3 cut(s) 199, 419, 856
MaeI CTAG 2 cut(s) 222, 858
MaeII ACGT 3 cut(s) 133, 159, 411
MaeIII GTNAC 1 cut(s) 776
MalI GATC 6 cut(s) 456, 480, 563, 744, 838, 886
MboI GATC 6 cut(s) 454, 478, 561, 742, 836, 884
MboII GAAGA 6 cut(s) 342, 537, 602, 949, 952, 955
MflI RGATCY 1 cut(s) 561
MhlI GDGCHC 1 cut(s) 300
MlyI GAGTC 4 cut(s) 4, 275, 280, 534
MmeI TCCRAC 2 cut(s) 229, 814
MseI TTAA 3 cut(s) 39, 318, 492
MspA1I CMGCKG 1 cut(s) 881
MspI CCGG 5 cut(s) 54, 277, 363, 506, 852
MspR9I CCNGG 7 cut(s) 60, 194, 278, 293, 507, 537, 852
MvaI CCWGG 4 cut(s) 60, 194, 293, 537
MvnI CGCG 1 cut(s) 872
MwoI GCNNNNNNNGC 2 cut(s) 242, 878
NciI CCSGG 3 cut(s) 278, 507, 852
NcoI CCATGG 1 cut(s) 237
NdeI CATATG 1 cut(s) 753
NdeII GATC 6 cut(s) 454, 478, 561, 742, 836, 884
NlaIII CATG 3 cut(s) 80, 186, 241
NlaIV GGNNCC 2 cut(s) 52, 253
NmeAIII GCCGAG 1 cut(s) 957
NmuCI GTSAC 1 cut(s) 776
PaeR7I CTCGAG 1 cut(s) 542
PfeI GAWTC 2 cut(s) 401, 579
PflFI GACNNNGTC 2 cut(s) 184, 602
PflMI CCANNNNNTGG 1 cut(s) 336
PfoI TCCNGGA 3 cut(s) 192, 505, 535
PinAI ACCGGT 1 cut(s) 362
PkrI GCNGC 3 cut(s) 7, 244, 880
PleI GAGTC 4 cut(s) 4, 275, 280, 534
PmaCI CACGTG 1 cut(s) 134
PmlI CACGTG 1 cut(s) 134
PpsI GAGTC 4 cut(s) 4, 275, 280, 534
Ppu21I YACGTR 1 cut(s) 134
Psp124BI GAGCTC 1 cut(s) 300
Psp6I CCWGG 4 cut(s) 58, 192, 291, 535
PspCI CACGTG 1 cut(s) 134
PspGI CCWGG 4 cut(s) 58, 192, 291, 535
PspN4I GGNNCC 2 cut(s) 52, 253
PspPI GGNCC 5 cut(s) 56, 251, 365, 533, 854
PspXI VCTCGAGB 1 cut(s) 542
PsrI GAACNNNNNNTAC 2 cut(s) 365, 397
PstI CTGCAG 1 cut(s) 247
PstNI CAGNNNCTG 1 cut(s) 712
PsuI RGATCY 1 cut(s) 561
PsyI GACNNNGTC 2 cut(s) 184, 602
PvuII CAGCTG 1 cut(s) 881
SacI GAGCTC 1 cut(s) 300
SaqAI TTAA 3 cut(s) 39, 318, 492
SatI GCNGC 3 cut(s) 6, 243, 879
Sau3AI GATC 6 cut(s) 454, 478, 561, 742, 836, 884
Sau96I GGNCC 5 cut(s) 56, 251, 365, 533, 854
SchI GAGTC 4 cut(s) 4, 275, 280, 534
ScrFI CCNGG 7 cut(s) 60, 194, 278, 293, 507, 537, 852
SduI GDGCHC 1 cut(s) 300
SfaNI GCATC 3 cut(s) 199, 419, 856
SfcI CTRYAG 2 cut(s) 81, 243
Sfr274I CTCGAG 1 cut(s) 542
SinI GGWCC 5 cut(s) 56, 251, 365, 533, 854
SlaI CTCGAG 1 cut(s) 542
SmlI CTYRAG 2 cut(s) 542, 983
SmoI CTYRAG 2 cut(s) 542, 983
SsiI CCGC 5 cut(s) 398, 432, 531, 692, 921
SspMI CTAG 2 cut(s) 222, 858
SstI GAGCTC 1 cut(s) 300
StyD4I CCNGG 7 cut(s) 58, 192, 276, 291, 505, 535, 850
StyI CCWWGG 1 cut(s) 237
TaaI ACNGT 3 cut(s) 314, 611, 666
TaiI ACGT 3 cut(s) 136, 162, 414
TaqI TCGA 6 cut(s) 165, 171, 284, 543, 615, 686
TfiI GAWTC 2 cut(s) 401, 579
Tru1I TTAA 3 cut(s) 39, 318, 492
Tru9I TTAA 3 cut(s) 39, 318, 492
TscAI CASTG 2 cut(s) 341, 614
TseFI GTSAC 1 cut(s) 776
TseI GCWGC 3 cut(s) 5, 242, 878
Tsp45I GTSAC 1 cut(s) 776
TspRI CASTG 2 cut(s) 341, 614
Tth111I GACNNNGTC 2 cut(s) 184, 602
Van91I CCANNNNNTGG 1 cut(s) 336
VpaK11BI GGWCC 5 cut(s) 56, 251, 365, 533, 854
XagI CCTNNNNNAGG 1 cut(s) 225
XcmI CCANNNNNNNNNTGG 1 cut(s) 200
XhoI CTCGAG 1 cut(s) 542
XspI CTAG 2 cut(s) 222, 858
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.