RLG00000020873

Nitrile-specifier protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0005031
Physical Location & Seq
Forward (+)
69715801 .. 69718068
2268 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020873

Sequence Viewer

Length: 990 bp
ATGGCTCTCAGGCAAGGCAAGTGGATCAAGCTTGATCAAGGTGGAACTGGGCCTGGAGCAAGAAGCTCACATGCCATAACCCTTGTTGGACAAAAGGCCTATGCCTTTGGTGGCGAATTCCAGCCACGTGTCCCGGTGGACAACAAGATGCACGTGTTCGACCTCAAAGAGCTGACGTGGTCTGCAATTTCCGGCACCGGCGATATCCCACCGCCACGTGTCGGTGTGACGATGGCAACTATTGGAAAAACCATTTATGTATTTGGTGGCAGGGACAATGAGCATAAGGAGCTCAATGAGCTTTACTCCTTCGACACATCTACAAACAAGTGGACCCTAATTTCCAGTGGGGATGCAGGCCCCCCTCACCGGAGCTACCACTCGGTGACTACCGACGACCGTCATGTGTATATTTTTGGGGGCTGCGGAGTGGCCGGCCGCCTCAATGATCTATGGGCTTACGACGTTGTTGATCAAAAGTGGATCCAATACCCAACACCGGGTGACAATTTGAAGGGAAGAGGTGGGCCTGGACTCCTAGTGTCCCAAGGAAAGATATGGGTTGTGTACGGCTTTGCTGGAGAGGAAGCAGATGATGTACACACATTTGATCTAGCCCATAAAGAGTGGACCCAAGTTGAGACAAGCGGGGAGAAACCAACTGCCCGAAGCGTATTTTCTACTGTTGCAATTGGGAAATACATAATTGTGTACGGTGGAGAAGTTGACCCCAGTGATCTAGGTCACATGGGTGCCGGAAAGTTCACCGCTCAAGCTTATGCACTGGACACAGAGACATTGCTGTGGAAGCAACTGGATGATGGCTTGGAGTCGAGCCATCATCCTGGACCTCGTGGGTGGTGTGCATTTACGGGTGGGGAAAGAGATGGCCGAGTAGGACTTTTGGTCTATGGTGGGAATTCGCCGAGTAATGATCGGCTTGATGACATTTTCTTTTTCACTCCTTATTTCGAAGCAGACGAAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kelch_1 Kelch_3 Kelch_4

Protein Analysis

330

Amino Acids

35.98

Weight (kDa)

5.44

Isoelectric Point (pI)

24.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_ATRN-LZTR1 PF24981 3 - 82 1.5e-08 Attractin/LZTR1 beta-propeller
Beta-prop_Calicin PF13964 6 - 273 1.8e-10 Calicin, beta-propeller domain
Beta-prop_FBX42 PF13415 7 - 111 5.7e-20 FBX42, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 23 - 240 2.7e-73 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
NANM PF24996 53 - 142 9.2e-06 N-acetylneuraminate epimerase
Kelch_HCF PF13854 67 - 167 9e-08 Host cell factor, Kelch-repeats domain
Kelch_FKB95 PF25210 70 - 187 1.2e-07 FKB95, Kelch-repeats domain
Kelch_1 PF01344 72 - 116 7.2e-12 Kelch motif
Beta-prop_ATRN-LZTR1 PF24981 85 - 164 2.5e-15 Attractin/LZTR1 beta-propeller
Beta-prop_FBX42 PF13415 96 - 195 9.2e-16 FBX42, beta-propeller domain
Kelch_1 PF01344 123 - 167 2e-10 Kelch motif
Beta-prop_FBX42 PF13415 127 - 321 1.2e-22 FBX42, beta-propeller domain
Kelch_HCF PF13854 184 - 289 2.1e-07 Host cell factor, Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 186 - 317 1.2e-13 Attractin/LZTR1 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 194, 752
AccBSI CCGCTC 1 cut(s) 770
AciI CCGC 5 cut(s) 212, 426, 439, 648, 768
AclWI GGATC 3 cut(s) 32, 478, 491
AcoI YGGCCR 3 cut(s) 432, 436, 889
AcsI RAATTY 2 cut(s) 116, 919
AcvI CACGTG 3 cut(s) 128, 154, 218
AdeI CACNNNGTG 2 cut(s) 385, 503
AfaI GTAC 3 cut(s) 569, 600, 713
AfiI CCNNNNNNNGG 4 cut(s) 221, 369, 499, 500
AflIII ACRYGT 3 cut(s) 127, 153, 217
AgsI TTSAA 1 cut(s) 514
AhdI GACNNNNNGTC 1 cut(s) 905
AjiI CACGTC 1 cut(s) 177
AjnI CCWGG 3 cut(s) 52, 529, 844
AleI CACNNNNGTG 1 cut(s) 750
AluBI AGCT 7 cut(s) 31, 66, 172, 292, 301, 375, 776
AluI AGCT 7 cut(s) 31, 66, 172, 292, 301, 375, 776
Alw21I GWGCWC 1 cut(s) 294
Alw26I GTCTC 2 cut(s) 635, 788
AlwI GGATC 3 cut(s) 32, 478, 491
AoxI GGCC 7 cut(s) 50, 96, 358, 432, 436, 527, 889
ApeKI GCWGC 1 cut(s) 423
ApoI RAATTY 2 cut(s) 116, 919
AspS9I GGNCC 6 cut(s) 50, 333, 359, 527, 630, 848
AsuC2I CCSGG 2 cut(s) 134, 501
AsuHPI GGTGA 4 cut(s) 359, 397, 515, 757
AsuII TTCGAA 1 cut(s) 972
AvaII GGWCC 3 cut(s) 333, 630, 848
BamHI GGATCC 1 cut(s) 483
BanI GGYRCC 2 cut(s) 194, 752
BanII GRGCYC 1 cut(s) 294
BauI CACGAG 1 cut(s) 852
BbrPI CACGTG 3 cut(s) 128, 154, 218
Bbv12I GWGCWC 1 cut(s) 294
BbvI GCAGC 1 cut(s) 410
BccI CCATC 4 cut(s) 226, 815, 846, 881
BceAI ACGGC 1 cut(s) 586
BciT130I CCWGG 3 cut(s) 54, 531, 846
BclI TGATCA 2 cut(s) 34, 472
BcnI CCSGG 2 cut(s) 134, 501
BcoDI GTCTC 2 cut(s) 635, 788
BfaI CTAG 3 cut(s) 539, 614, 740
BisI GCNGC 2 cut(s) 424, 439
BlsI GCNGC 2 cut(s) 425, 440
Bme1390I CCNGG 5 cut(s) 54, 134, 501, 531, 846
Bme18I GGWCC 3 cut(s) 333, 630, 848
BmeRI GACNNNNNGTC 1 cut(s) 905
BmgBI CACGTC 1 cut(s) 177
BmgT120I GGNCC 6 cut(s) 50, 333, 359, 527, 630, 848
BmiI GGNNCC 6 cut(s) 196, 335, 361, 485, 632, 754
BmrFI CCNGG 5 cut(s) 54, 134, 501, 531, 846
BmrI ACTGGG 2 cut(s) 57, 726
BmsI GCATC 2 cut(s) 138, 343
BmuI ACTGGG 2 cut(s) 57, 726
BoxI GACNNNNGTC 1 cut(s) 399
BplI GAGNNNNNCTC 2 cut(s) 290, 322
BpmI CTGGAG 2 cut(s) 75, 600
Bpu14I TTCGAA 1 cut(s) 972
BpuEI CTTGAG 1 cut(s) 756
BpuMI CCSGG 2 cut(s) 134, 501
BsaAI YACGTR 3 cut(s) 128, 154, 218
BsaJI CCNNGG 1 cut(s) 547
BsaWI WCCGGW 1 cut(s) 369
Bsc4I CCNNNNNNNGG 4 cut(s) 221, 369, 499, 500
Bse118I RCCGGY 2 cut(s) 197, 434
Bse1I ACTGG 5 cut(s) 52, 345, 732, 789, 819
Bse3DI GCAATG 1 cut(s) 797
BseBI CCWGG 3 cut(s) 54, 531, 846
BseDI CCNNGG 1 cut(s) 547
BseGI GGATG 3 cut(s) 358, 823, 841
BseLI CCNNNNNNNGG 4 cut(s) 221, 369, 499, 500
BseMI GCAATG 1 cut(s) 797
BseMII CTCAG 1 cut(s) 22
BseNI ACTGG 5 cut(s) 52, 345, 732, 789, 819
BseX3I CGGCCG 1 cut(s) 436
BseXI GCAGC 1 cut(s) 410
Bsh1285I CGRYCG 2 cut(s) 400, 439
BshFI GGCC 7 cut(s) 52, 98, 360, 434, 438, 529, 891
BshNI GGYRCC 2 cut(s) 194, 752
BsiEI CGRYCG 2 cut(s) 400, 439
BsiHKAI GWGCWC 1 cut(s) 294
BsiSI CCGG 7 cut(s) 134, 192, 198, 370, 435, 500, 756
BslFI GGGAC 3 cut(s) 116, 287, 529
BslI CCNNNNNNNGG 4 cut(s) 221, 369, 499, 500
BsmAI GTCTC 2 cut(s) 635, 788
BsmFI GGGAC 3 cut(s) 116, 287, 529
BsnI GGCC 7 cut(s) 52, 98, 360, 434, 438, 529, 891
Bsp119I TTCGAA 1 cut(s) 972
Bsp1286I GDGCHC 1 cut(s) 294
Bsp1407I TGTACA 1 cut(s) 598
Bsp143I GATC 8 cut(s) 24, 34, 448, 472, 483, 610, 736, 934
BspACI CCGC 5 cut(s) 212, 426, 439, 648, 768
BspANI GGCC 7 cut(s) 52, 98, 360, 434, 438, 529, 891
BspCNI CTCAG 1 cut(s) 21
BspLI GGNNCC 6 cut(s) 196, 335, 361, 485, 632, 754
BspPI GGATC 3 cut(s) 32, 478, 491
BspT104I TTCGAA 1 cut(s) 972
BspT107I GGYRCC 2 cut(s) 194, 752
BsrBI CCGCTC 1 cut(s) 770
BsrDI GCAATG 1 cut(s) 797
BsrFI RCCGGY 2 cut(s) 197, 434
BsrGI TGTACA 1 cut(s) 598
BsrI ACTGG 5 cut(s) 52, 345, 732, 789, 819
BssAI RCCGGY 2 cut(s) 197, 434
BssECI CCNNGG 1 cut(s) 547
BssMI GATC 8 cut(s) 24, 34, 448, 472, 483, 610, 736, 934
BssSI CACGAG 1 cut(s) 852
BssT1I CCWWGG 1 cut(s) 547
Bst2BI CACGAG 1 cut(s) 852
Bst2UI CCWGG 3 cut(s) 54, 531, 846
Bst4CI ACNGT 3 cut(s) 401, 685, 716
Bst6I CTCTTC 1 cut(s) 514
BstAUI TGTACA 1 cut(s) 598
BstBAI YACGTR 3 cut(s) 128, 154, 218
BstBI TTCGAA 1 cut(s) 972
BstC8I GCNNGC 2 cut(s) 358, 436
BstDEI CTNAG 1 cut(s) 8
BstF5I GGATG 3 cut(s) 358, 823, 841
BstKTI GATC 8 cut(s) 27, 37, 451, 475, 486, 613, 739, 937
BstMAI GTCTC 2 cut(s) 635, 788
BstMBI GATC 8 cut(s) 24, 34, 448, 472, 483, 610, 736, 934
BstMCI CGRYCG 2 cut(s) 400, 439
BstMWI GCNNNNNNNGC 3 cut(s) 289, 298, 808
BstNI CCWGG 3 cut(s) 54, 531, 846
BstNSI RCATGY 1 cut(s) 74
BstPAI GACNNNNGTC 1 cut(s) 399
BstSCI CCNGG 5 cut(s) 52, 132, 499, 529, 844
BstV1I GCAGC 1 cut(s) 410
BstX2I RGATCY 1 cut(s) 483
BstXI CCANNNNNNTGG 1 cut(s) 845
BstYI RGATCY 1 cut(s) 483
BstZI CGGCCG 1 cut(s) 436
BsuRI GGCC 7 cut(s) 52, 98, 360, 434, 438, 529, 891
BtrI CACGTC 1 cut(s) 177
BtsCI GGATG 3 cut(s) 358, 823, 841
BtsIMutI CAGTG 3 cut(s) 352, 739, 782
Cac8I GCNNGC 2 cut(s) 358, 436
Cfr10I RCCGGY 2 cut(s) 197, 434
Cfr13I GGNCC 6 cut(s) 50, 333, 359, 527, 630, 848
Csp6I GTAC 3 cut(s) 568, 599, 712
CspCI CAANNNNNGTGG 1 cut(s) 37
CviAII CATG 3 cut(s) 71, 404, 748
CviQI GTAC 3 cut(s) 568, 599, 712
DdeI CTNAG 1 cut(s) 8
DpnI GATC 8 cut(s) 26, 36, 450, 474, 485, 612, 738, 936
DpnII GATC 8 cut(s) 24, 34, 448, 472, 483, 610, 736, 934
DraIII CACNNNGTG 2 cut(s) 385, 503
DriI GACNNNNNGTC 1 cut(s) 905
EaeI YGGCCR 3 cut(s) 432, 436, 889
EagI CGGCCG 1 cut(s) 436
Eam1104I CTCTTC 1 cut(s) 514
Eam1105I GACNNNNNGTC 1 cut(s) 905
EarI CTCTTC 1 cut(s) 514
Ecl136II GAGCTC 1 cut(s) 292
EclXI CGGCCG 1 cut(s) 436
Eco130I CCWWGG 1 cut(s) 547
Eco147I AGGCCT 1 cut(s) 98
Eco24I GRGCYC 1 cut(s) 294
Eco32I GATATC 1 cut(s) 205
Eco47I GGWCC 3 cut(s) 333, 630, 848
Eco52I CGGCCG 1 cut(s) 436
Eco53kI GAGCTC 1 cut(s) 292
Eco72I CACGTG 3 cut(s) 128, 154, 218
EcoICRI GAGCTC 1 cut(s) 292
EcoO109I RGGNCCY 1 cut(s) 359
EcoRI GAATTC 2 cut(s) 116, 919
EcoRII CCWGG 3 cut(s) 52, 529, 844
EcoRV GATATC 1 cut(s) 205
EcoT14I CCWWGG 1 cut(s) 547
EcoT38I GRGCYC 1 cut(s) 294
ErhI CCWWGG 1 cut(s) 547
FaeI CATG 3 cut(s) 74, 407, 751
FaqI GGGAC 3 cut(s) 116, 287, 529
FatI CATG 3 cut(s) 70, 403, 747
FauI CCCGC 1 cut(s) 641
FbaI TGATCA 2 cut(s) 34, 472
Fnu4HI GCNGC 2 cut(s) 424, 439
FokI GGATG 3 cut(s) 365, 828, 830
FriOI GRGCYC 1 cut(s) 294
FseI GGCCGGCC 1 cut(s) 438
Fsp4HI GCNGC 2 cut(s) 424, 439
FspBI CTAG 3 cut(s) 539, 614, 740
GluI GCNGC 2 cut(s) 424, 439
GsuI CTGGAG 2 cut(s) 75, 600
HaeIII GGCC 7 cut(s) 52, 98, 360, 434, 438, 529, 891
HapII CCGG 7 cut(s) 134, 192, 198, 370, 435, 500, 756
Hin1II CATG 3 cut(s) 74, 407, 751
HincII GTYRAC 1 cut(s) 727
HindII GTYRAC 1 cut(s) 727
HindIII AAGCTT 2 cut(s) 29, 774
HinfI GANTC 2 cut(s) 534, 830
HpaII CCGG 7 cut(s) 134, 192, 198, 370, 435, 500, 756
HphI GGTGA 4 cut(s) 359, 397, 515, 757
Hpy166II GTNNAC 8 cut(s) 139, 333, 568, 601, 630, 712, 727, 765
Hpy8I GTNNAC 8 cut(s) 139, 333, 568, 601, 630, 712, 727, 765
Hpy99I CGWCG 2 cut(s) 398, 467
HpyAV CCTTC 2 cut(s) 319, 508
HpyCH4III ACNGT 3 cut(s) 401, 685, 716
HpyCH4IV ACGT 5 cut(s) 127, 153, 176, 217, 465
HpyCH4V TGCA 6 cut(s) 151, 185, 356, 689, 782, 866
HpyF10VI GCNNNNNNNGC 3 cut(s) 289, 298, 808
HpyF3I CTNAG 1 cut(s) 8
HpySE526I ACGT 5 cut(s) 127, 153, 176, 217, 465
Hsp92II CATG 3 cut(s) 74, 407, 751
KroI GCCGGC 1 cut(s) 434
KroNI GCCGGC 1 cut(s) 436
Ksp22I TGATCA 2 cut(s) 34, 472
Kzo9I GATC 8 cut(s) 24, 34, 448, 472, 483, 610, 736, 934
LmnI GCTCC 3 cut(s) 56, 289, 372
Lsp1109I GCAGC 1 cut(s) 410
LweI GCATC 2 cut(s) 138, 343
MaeI CTAG 3 cut(s) 539, 614, 740
MaeII ACGT 5 cut(s) 127, 153, 176, 217, 465
MaeIII GTNAC 4 cut(s) 226, 385, 503, 743
MalI GATC 8 cut(s) 26, 36, 450, 474, 485, 612, 738, 936
MbiI CCGCTC 1 cut(s) 770
MboI GATC 8 cut(s) 24, 34, 448, 472, 483, 610, 736, 934
MboII GAAGA 1 cut(s) 531
MfeI CAATTG 1 cut(s) 690
MflI RGATCY 1 cut(s) 483
MhlI GDGCHC 1 cut(s) 294
MluCI AATT 7 cut(s) 116, 186, 339, 508, 690, 705, 919
MlyI GAGTC 2 cut(s) 528, 839
MmeI TCCRAC 1 cut(s) 67
MnlI CCTC 6 cut(s) 173, 375, 452, 515, 577, 861
MroNI GCCGGC 1 cut(s) 434
MslI CAYNNNNRTG 3 cut(s) 707, 750, 802
MspI CCGG 7 cut(s) 134, 192, 198, 370, 435, 500, 756
MspR9I CCNGG 5 cut(s) 54, 134, 501, 531, 846
MunI CAATTG 1 cut(s) 690
MvaI CCWGG 3 cut(s) 54, 531, 846
MwoI GCNNNNNNNGC 3 cut(s) 289, 298, 808
NaeI GCCGGC 1 cut(s) 436
NciI CCSGG 2 cut(s) 134, 501
NdeII GATC 8 cut(s) 24, 34, 448, 472, 483, 610, 736, 934
NgoMIV GCCGGC 1 cut(s) 434
NlaIII CATG 3 cut(s) 74, 407, 751
NlaIV GGNNCC 6 cut(s) 196, 335, 361, 485, 632, 754
NmeAIII GCCGAG 2 cut(s) 917, 951
NmuCI GTSAC 4 cut(s) 226, 385, 503, 743
NspI RCATGY 1 cut(s) 74
NspV TTCGAA 1 cut(s) 972
OliI CACNNNNGTG 1 cut(s) 750
PceI AGGCCT 1 cut(s) 98
PcsI WCGNNNNNNNCGW 1 cut(s) 978
PdiI GCCGGC 1 cut(s) 436
PflFI GACNNNGTC 1 cut(s) 178
PfoI TCCNGGA 1 cut(s) 844
PkrI GCNGC 2 cut(s) 425, 440
PleI GAGTC 2 cut(s) 528, 838
PmaCI CACGTG 3 cut(s) 128, 154, 218
PmlI CACGTG 3 cut(s) 128, 154, 218
PpsI GAGTC 2 cut(s) 528, 838
Ppu21I YACGTR 3 cut(s) 128, 154, 218
PshAI GACNNNNGTC 1 cut(s) 399
Psp124BI GAGCTC 1 cut(s) 294
Psp6I CCWGG 3 cut(s) 52, 529, 844
PspCI CACGTG 3 cut(s) 128, 154, 218
PspGI CCWGG 3 cut(s) 52, 529, 844
PspN4I GGNNCC 6 cut(s) 196, 335, 361, 485, 632, 754
PspPI GGNCC 6 cut(s) 50, 333, 359, 527, 630, 848
PsuI RGATCY 1 cut(s) 483
PsyI GACNNNGTC 1 cut(s) 178
RigI GGCCGGCC 1 cut(s) 438
RsaI GTAC 3 cut(s) 569, 600, 713
RsaNI GTAC 3 cut(s) 568, 599, 712
RseI CAYNNNNRTG 3 cut(s) 707, 750, 802
SacI GAGCTC 1 cut(s) 294
SatI GCNGC 2 cut(s) 424, 439
Sau3AI GATC 8 cut(s) 24, 34, 448, 472, 483, 610, 736, 934
Sau96I GGNCC 6 cut(s) 50, 333, 359, 527, 630, 848
SchI GAGTC 2 cut(s) 528, 839
ScrFI CCNGG 5 cut(s) 54, 134, 501, 531, 846
SduI GDGCHC 1 cut(s) 294
SfaNI GCATC 2 cut(s) 138, 343
SfuI TTCGAA 1 cut(s) 972
SgrAI CRCCGGYG 1 cut(s) 197
SinI GGWCC 3 cut(s) 333, 630, 848
SmiMI CAYNNNNRTG 3 cut(s) 707, 750, 802
SmlI CTYRAG 1 cut(s) 771
SmoI CTYRAG 1 cut(s) 771
Sse9I AATT 7 cut(s) 116, 186, 339, 508, 690, 705, 919
SseBI AGGCCT 1 cut(s) 98
SsiI CCGC 5 cut(s) 212, 426, 439, 648, 768
SspMI CTAG 3 cut(s) 539, 614, 740
SstI GAGCTC 1 cut(s) 294
StuI AGGCCT 1 cut(s) 98
StyD4I CCNGG 5 cut(s) 52, 132, 499, 529, 844
StyI CCWWGG 1 cut(s) 547
TaaI ACNGT 3 cut(s) 401, 685, 716
TaiI ACGT 5 cut(s) 130, 156, 179, 220, 468
TaqI TCGA 4 cut(s) 159, 312, 833, 972
TasI AATT 7 cut(s) 116, 186, 339, 508, 690, 705, 919
TatI WGTACW 1 cut(s) 598
TauI GCSGC 1 cut(s) 441
TscAI CASTG 3 cut(s) 352, 739, 789
TseFI GTSAC 4 cut(s) 226, 385, 503, 743
TseI GCWGC 1 cut(s) 423
Tsp45I GTSAC 4 cut(s) 226, 385, 503, 743
TspRI CASTG 3 cut(s) 352, 739, 789
Tth111I GACNNNGTC 1 cut(s) 178
VpaK11BI GGWCC 3 cut(s) 333, 630, 848
XapI RAATTY 2 cut(s) 116, 919
XceI RCATGY 1 cut(s) 74
XspI CTAG 3 cut(s) 539, 614, 740
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.