Rroxscaffold_2G00093420

Nitrile-specifier protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0005031
Physical Location & Seq
Reverse (-)
14770078 .. 14772973
2896 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00093420.1

Sequence Viewer

Length: 1086 bp
ATGTTAAAACCACATTCCTTTGATATCCAGACGCAGGATTCAGAGATTGCGGCAGCACAGTTGCAAGAGCAGTCATATCTCTATGATAGCGAAATTTTTGCAAGAGCAGTCATAACATATTCTGCAAGCTCTGGTTTGCAGAGCTGTGACATTATACACTACCCAGATGTACCAAGTATCAAGGATTTGGAGAAACCCAATTCATCAGTGACTCCCTCTCTTAAAGGCTCTTCGTCATCATCCAAGCATGGCTCTCAGGCAAGGCGAGTGGATAAAGAAGGCCTATGCCTTTGGTGGCGAATTCCAGCCACGTGTCCCGGTGGACAACAAGATGCACGTGTTCGACCTCAAAGAGCTGACGTGGTCTGCAATTTCCGGCACCGGCGATATCCCACCGCCACGTGTCGGTGTGACGATGGCAAACTATTGGAAAAACCATTTATGTATTTGGTGGCAGGGACAAATGAGCACAAGGAGCTGAATGAGCTTTACTCCTTTGACACATCTACAAACAAGTGGACCCTAATTTCCCAGTGGGGATGCAGGCCCCCGCACCGGAGCTACCACTCGGTGACAACCGACGACCGTCAAGTGTATATTTTTGGGGGCTGCGGAGTGGCCGGCCGCCTCAATGATCTATGGGCTTACGACGTTGTTGATCAAAAGTGGATCCAATACCCAACACCGGGTGACAATTTGAAGGGAAGAGGTGGGCCTGGACTCCTAGTCTCCCAAGGAAAGATATGGGTTGTGTATGGCTTCGCCGGAGAGGAAGCCGATGATGTTCACACATTTGATCTAGCCCATAAAGAGTGGACCCAAGTTGAGACAAGTGGGGAGAAACCGACCGCCCGAAGCGTATTTTCTACTGTTGCAATTGGGAAATACATAATTGTGTACGGTGGAGAAGTTGACCCCAGTGATCTAGGTCACATGGGTGCCGGAAAGTTTACAGCTCAAGCTTATGCACACTGGACACAGAGACATTGCTGTGGAAGCAATTGGATGATGGCTTGGAGTCGAGCCATCATCCTGGACCTCGTGGGTGGTGTGCATTTACGGGTGGGGAACGAGATGGCCGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kelch_1 Kelch_3 Kelch_4

Protein Analysis

361

Amino Acids

40.26

Weight (kDa)

6.35

Isoelectric Point (pI)

38.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_KLHDC2_KLHL20_DRC7 PF24681 147 - 302 1.3e-47 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 148 - 226 4.8e-13 Attractin/LZTR1 beta-propeller
Beta-prop_FBX42 PF13415 157 - 257 1.7e-15 FBX42, beta-propeller domain
Kelch_1 PF01344 185 - 229 1.7e-10 Kelch motif
Kelch_HCF PF13854 245 - 305 2.3e-06 Host cell factor, Kelch-repeats domain
Beta-prop_FBX42 PF13415 247 - 318 2.5e-08 FBX42, beta-propeller domain
Beta-prop_ATRN-LZTR1 PF24981 248 - 306 9.3e-08 Attractin/LZTR1 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 378, 938
AciI CCGC 6 cut(s) 50, 396, 551, 612, 625, 849
AclWI GGATC 2 cut(s) 664, 677
AcoI YGGCCR 3 cut(s) 618, 622, 1077
AcsI RAATTY 2 cut(s) 93, 300
AcvI CACGTG 3 cut(s) 312, 338, 402
AdeI CACNNNGTG 2 cut(s) 571, 689
AfaI GTAC 2 cut(s) 171, 899
AfiI CCNNNNNNNGG 5 cut(s) 34, 405, 555, 685, 686
AflIII ACRYGT 3 cut(s) 311, 337, 401
AgsI TTSAA 1 cut(s) 700
AhdI GACNNNNNGTC 1 cut(s) 725
AjiI CACGTC 1 cut(s) 361
AjnI CCWGG 2 cut(s) 715, 1032
AleI CACNNNNGTG 1 cut(s) 936
AluBI AGCT 8 cut(s) 129, 144, 356, 478, 487, 561, 956, 962
AluI AGCT 8 cut(s) 129, 144, 356, 478, 487, 561, 956, 962
Alw21I GWGCWC 1 cut(s) 471
Alw26I GTCTC 3 cut(s) 733, 821, 976
AlwI GGATC 2 cut(s) 664, 677
AoxI GGCC 6 cut(s) 280, 545, 618, 622, 713, 1077
ApeKI GCWGC 2 cut(s) 53, 609
ApoI RAATTY 2 cut(s) 93, 300
AspS9I GGNCC 5 cut(s) 519, 546, 713, 816, 1036
AsuC2I CCSGG 2 cut(s) 318, 687
AsuHPI GGTGA 2 cut(s) 583, 701
AvaII GGWCC 3 cut(s) 519, 816, 1036
BamHI GGATCC 1 cut(s) 669
BanI GGYRCC 2 cut(s) 378, 938
BauI CACGAG 1 cut(s) 1040
BbrPI CACGTG 3 cut(s) 312, 338, 402
Bbv12I GWGCWC 1 cut(s) 471
BbvI GCAGC 2 cut(s) 65, 596
BccI CCATC 4 cut(s) 410, 1003, 1034, 1069
BcgI CGANNNNNNTGC 2 cut(s) 80, 114
BciT130I CCWGG 2 cut(s) 717, 1034
BclI TGATCA 1 cut(s) 658
BcnI CCSGG 2 cut(s) 318, 687
BcoDI GTCTC 3 cut(s) 733, 821, 976
BfaI CTAG 3 cut(s) 725, 800, 926
BisI GCNGC 4 cut(s) 51, 54, 610, 625
BlsI GCNGC 4 cut(s) 52, 55, 611, 626
Bme1390I CCNGG 4 cut(s) 318, 687, 717, 1034
Bme18I GGWCC 3 cut(s) 519, 816, 1036
BmeRI GACNNNNNGTC 1 cut(s) 725
BmgBI CACGTC 1 cut(s) 361
BmgT120I GGNCC 5 cut(s) 519, 546, 713, 816, 1036
BmiI GGNNCC 6 cut(s) 380, 521, 548, 671, 818, 940
BmrFI CCNGG 4 cut(s) 318, 687, 717, 1034
BmrI ACTGGG 2 cut(s) 526, 912
BmsI GCATC 2 cut(s) 322, 530
BmuI ACTGGG 2 cut(s) 526, 912
BoxI GACNNNNGTC 1 cut(s) 585
BplI GAGNNNNNCTC 2 cut(s) 476, 508
BpuEI CTTGAG 1 cut(s) 942
BpuMI CCSGG 2 cut(s) 318, 687
BsaAI YACGTR 3 cut(s) 312, 338, 402
BsaJI CCNNGG 1 cut(s) 733
BsaWI WCCGGW 1 cut(s) 555
Bsc4I CCNNNNNNNGG 5 cut(s) 34, 405, 555, 685, 686
Bse118I RCCGGY 2 cut(s) 381, 620
Bse1I ACTGG 3 cut(s) 532, 918, 977
Bse3DI GCAATG 1 cut(s) 985
BseBI CCWGG 2 cut(s) 717, 1034
BseDI CCNNGG 1 cut(s) 733
BseGI GGATG 4 cut(s) 239, 545, 1011, 1029
BseLI CCNNNNNNNGG 5 cut(s) 34, 405, 555, 685, 686
BseMI GCAATG 1 cut(s) 985
BseMII CTCAG 1 cut(s) 269
BseNI ACTGG 3 cut(s) 532, 918, 977
BseX3I CGGCCG 1 cut(s) 622
BseXI GCAGC 2 cut(s) 65, 596
Bsh1285I CGRYCG 3 cut(s) 586, 625, 849
BshFI GGCC 6 cut(s) 282, 547, 620, 624, 715, 1079
BshNI GGYRCC 2 cut(s) 378, 938
BsiEI CGRYCG 3 cut(s) 586, 625, 849
BsiHKAI GWGCWC 1 cut(s) 471
BsiSI CCGG 8 cut(s) 318, 376, 382, 556, 621, 686, 765, 942
BslFI GGGAC 2 cut(s) 300, 472
BslI CCNNNNNNNGG 5 cut(s) 34, 405, 555, 685, 686
BsmAI GTCTC 3 cut(s) 733, 821, 976
BsmFI GGGAC 2 cut(s) 300, 472
BsnI GGCC 6 cut(s) 282, 547, 620, 624, 715, 1079
Bsp1286I GDGCHC 1 cut(s) 471
Bsp143I GATC 5 cut(s) 634, 658, 669, 796, 922
BspACI CCGC 6 cut(s) 50, 396, 551, 612, 625, 849
BspANI GGCC 6 cut(s) 282, 547, 620, 624, 715, 1079
BspCNI CTCAG 1 cut(s) 268
BspLI GGNNCC 6 cut(s) 380, 521, 548, 671, 818, 940
BspPI GGATC 2 cut(s) 664, 677
BspQI GCTCTTC 1 cut(s) 235
BspT107I GGYRCC 2 cut(s) 378, 938
BsrDI GCAATG 1 cut(s) 985
BsrFI RCCGGY 2 cut(s) 381, 620
BsrI ACTGG 3 cut(s) 532, 918, 977
BssAI RCCGGY 2 cut(s) 381, 620
BssECI CCNNGG 1 cut(s) 733
BssMI GATC 5 cut(s) 634, 658, 669, 796, 922
BssSI CACGAG 1 cut(s) 1040
BssT1I CCWWGG 1 cut(s) 733
Bst2BI CACGAG 1 cut(s) 1040
Bst2UI CCWGG 2 cut(s) 717, 1034
Bst4CI ACNGT 4 cut(s) 60, 587, 871, 902
Bst6I CTCTTC 2 cut(s) 235, 700
BstBAI YACGTR 3 cut(s) 312, 338, 402
BstC8I GCNNGC 3 cut(s) 127, 545, 622
BstDEI CTNAG 1 cut(s) 255
BstF5I GGATG 4 cut(s) 239, 545, 1011, 1029
BstKTI GATC 5 cut(s) 637, 661, 672, 799, 925
BstMAI GTCTC 3 cut(s) 733, 821, 976
BstMBI GATC 5 cut(s) 634, 658, 669, 796, 922
BstMCI CGRYCG 3 cut(s) 586, 625, 849
BstMWI GCNNNNNNNGC 3 cut(s) 475, 484, 996
BstNI CCWGG 2 cut(s) 717, 1034
BstPAI GACNNNNGTC 1 cut(s) 585
BstSCI CCNGG 4 cut(s) 316, 685, 715, 1032
BstV1I GCAGC 2 cut(s) 65, 596
BstX2I RGATCY 1 cut(s) 669
BstXI CCANNNNNNTGG 1 cut(s) 1033
BstYI RGATCY 1 cut(s) 669
BstZI CGGCCG 1 cut(s) 622
BsuRI GGCC 6 cut(s) 282, 547, 620, 624, 715, 1079
BtrI CACGTC 1 cut(s) 361
BtsCI GGATG 4 cut(s) 239, 545, 1011, 1029
BtsIMutI CAGTG 4 cut(s) 213, 539, 925, 970
Cac8I GCNNGC 3 cut(s) 127, 545, 622
Cfr10I RCCGGY 2 cut(s) 381, 620
Cfr13I GGNCC 5 cut(s) 519, 546, 713, 816, 1036
CseI GACGC 1 cut(s) 40
Csp6I GTAC 2 cut(s) 170, 898
CspCI CAANNNNNGTGG 2 cut(s) 249, 284
CviAII CATG 2 cut(s) 248, 934
CviQI GTAC 2 cut(s) 170, 898
DdeI CTNAG 1 cut(s) 255
DpnI GATC 5 cut(s) 636, 660, 671, 798, 924
DpnII GATC 5 cut(s) 634, 658, 669, 796, 922
DraIII CACNNNGTG 2 cut(s) 571, 689
DriI GACNNNNNGTC 1 cut(s) 725
EaeI YGGCCR 3 cut(s) 618, 622, 1077
EagI CGGCCG 1 cut(s) 622
Eam1104I CTCTTC 2 cut(s) 235, 700
Eam1105I GACNNNNNGTC 1 cut(s) 725
EarI CTCTTC 2 cut(s) 235, 700
EclXI CGGCCG 1 cut(s) 622
Eco130I CCWWGG 1 cut(s) 733
Eco147I AGGCCT 1 cut(s) 282
Eco32I GATATC 2 cut(s) 25, 389
Eco47I GGWCC 3 cut(s) 519, 816, 1036
Eco52I CGGCCG 1 cut(s) 622
Eco72I CACGTG 3 cut(s) 312, 338, 402
EcoO109I RGGNCCY 1 cut(s) 546
EcoRI GAATTC 1 cut(s) 300
EcoRII CCWGG 2 cut(s) 715, 1032
EcoRV GATATC 2 cut(s) 25, 389
EcoT14I CCWWGG 1 cut(s) 733
ErhI CCWWGG 1 cut(s) 733
FaeI CATG 2 cut(s) 251, 937
FaqI GGGAC 2 cut(s) 300, 472
FatI CATG 2 cut(s) 247, 933
FauI CCCGC 1 cut(s) 558
FbaI TGATCA 1 cut(s) 658
Fnu4HI GCNGC 4 cut(s) 51, 54, 610, 625
FokI GGATG 4 cut(s) 226, 552, 1016, 1018
FseI GGCCGGCC 1 cut(s) 624
Fsp4HI GCNGC 4 cut(s) 51, 54, 610, 625
FspBI CTAG 3 cut(s) 725, 800, 926
GluI GCNGC 4 cut(s) 51, 54, 610, 625
HaeIII GGCC 6 cut(s) 282, 547, 620, 624, 715, 1079
HapII CCGG 8 cut(s) 318, 376, 382, 556, 621, 686, 765, 942
HgaI GACGC 1 cut(s) 40
Hin1II CATG 2 cut(s) 251, 937
HincII GTYRAC 1 cut(s) 913
HindII GTYRAC 1 cut(s) 913
HindIII AAGCTT 1 cut(s) 960
HinfI GANTC 4 cut(s) 38, 211, 720, 1018
HpaII CCGG 8 cut(s) 318, 376, 382, 556, 621, 686, 765, 942
HphI GGTGA 2 cut(s) 583, 701
Hpy166II GTNNAC 7 cut(s) 323, 519, 787, 816, 898, 913, 951
Hpy188I TCNGA 1 cut(s) 43
Hpy188III TCNNGA 1 cut(s) 28
Hpy8I GTNNAC 7 cut(s) 323, 519, 787, 816, 898, 913, 951
Hpy99I CGWCG 2 cut(s) 584, 653
HpyAV CCTTC 2 cut(s) 272, 694
HpyCH4III ACNGT 4 cut(s) 60, 587, 871, 902
HpyCH4IV ACGT 5 cut(s) 311, 337, 360, 401, 651
HpyF10VI GCNNNNNNNGC 3 cut(s) 475, 484, 996
HpyF3I CTNAG 1 cut(s) 255
HpySE526I ACGT 5 cut(s) 311, 337, 360, 401, 651
Hsp92II CATG 2 cut(s) 251, 937
KroI GCCGGC 1 cut(s) 620
KroNI GCCGGC 1 cut(s) 622
Ksp22I TGATCA 1 cut(s) 658
Kzo9I GATC 5 cut(s) 634, 658, 669, 796, 922
LguI GCTCTTC 1 cut(s) 235
LmnI GCTCC 2 cut(s) 475, 558
Lsp1109I GCAGC 2 cut(s) 65, 596
LweI GCATC 2 cut(s) 322, 530
MaeI CTAG 3 cut(s) 725, 800, 926
MaeII ACGT 5 cut(s) 311, 337, 360, 401, 651
MaeIII GTNAC 6 cut(s) 146, 208, 410, 571, 689, 929
MalI GATC 5 cut(s) 636, 660, 671, 798, 924
MboI GATC 5 cut(s) 634, 658, 669, 796, 922
MboII GAAGA 2 cut(s) 222, 717
MfeI CAATTG 2 cut(s) 876, 1000
MflI RGATCY 1 cut(s) 669
MhlI GDGCHC 1 cut(s) 471
MluCI AATT 9 cut(s) 93, 199, 300, 370, 525, 694, 876, 891, 1000
MlyI GAGTC 3 cut(s) 205, 714, 1027
MnlI CCTC 6 cut(s) 226, 357, 638, 701, 763, 1049
MroNI GCCGGC 1 cut(s) 620
MseI TTAA 2 cut(s) 5, 222
MslI CAYNNNNRTG 3 cut(s) 893, 936, 990
MspI CCGG 8 cut(s) 318, 376, 382, 556, 621, 686, 765, 942
MspR9I CCNGG 4 cut(s) 318, 687, 717, 1034
MunI CAATTG 2 cut(s) 876, 1000
MvaI CCWGG 2 cut(s) 717, 1034
MwoI GCNNNNNNNGC 3 cut(s) 475, 484, 996
NaeI GCCGGC 1 cut(s) 622
NciI CCSGG 2 cut(s) 318, 687
NdeII GATC 5 cut(s) 634, 658, 669, 796, 922
NgoMIV GCCGGC 1 cut(s) 620
NlaIII CATG 2 cut(s) 251, 937
NlaIV GGNNCC 6 cut(s) 380, 521, 548, 671, 818, 940
NmuCI GTSAC 6 cut(s) 146, 208, 410, 571, 689, 929
OliI CACNNNNGTG 1 cut(s) 936
PceI AGGCCT 1 cut(s) 282
PciSI GCTCTTC 1 cut(s) 235
PcsI WCGNNNNNNNCGW 1 cut(s) 1077
PdiI GCCGGC 1 cut(s) 622
PfeI GAWTC 1 cut(s) 38
PflFI GACNNNGTC 1 cut(s) 362
PfoI TCCNGGA 1 cut(s) 1032
PkrI GCNGC 4 cut(s) 52, 55, 611, 626
PleI GAGTC 3 cut(s) 205, 714, 1026
PmaCI CACGTG 3 cut(s) 312, 338, 402
PmlI CACGTG 3 cut(s) 312, 338, 402
PpsI GAGTC 3 cut(s) 205, 714, 1026
Ppu21I YACGTR 3 cut(s) 312, 338, 402
PshAI GACNNNNGTC 1 cut(s) 585
Psp6I CCWGG 2 cut(s) 715, 1032
PspCI CACGTG 3 cut(s) 312, 338, 402
PspGI CCWGG 2 cut(s) 715, 1032
PspN4I GGNNCC 6 cut(s) 380, 521, 548, 671, 818, 940
PspPI GGNCC 5 cut(s) 519, 546, 713, 816, 1036
PsuI RGATCY 1 cut(s) 669
PsyI GACNNNGTC 1 cut(s) 362
RigI GGCCGGCC 1 cut(s) 624
RsaI GTAC 2 cut(s) 171, 899
RsaNI GTAC 2 cut(s) 170, 898
RseI CAYNNNNRTG 3 cut(s) 893, 936, 990
SapI GCTCTTC 1 cut(s) 235
SaqAI TTAA 2 cut(s) 5, 222
SatI GCNGC 4 cut(s) 51, 54, 610, 625
Sau3AI GATC 5 cut(s) 634, 658, 669, 796, 922
Sau96I GGNCC 5 cut(s) 519, 546, 713, 816, 1036
SchI GAGTC 3 cut(s) 205, 714, 1027
ScrFI CCNGG 4 cut(s) 318, 687, 717, 1034
SduI GDGCHC 1 cut(s) 471
SfaNI GCATC 2 cut(s) 322, 530
SgrAI CRCCGGYG 1 cut(s) 381
SinI GGWCC 3 cut(s) 519, 816, 1036
SmiMI CAYNNNNRTG 3 cut(s) 893, 936, 990
SmlI CTYRAG 1 cut(s) 957
SmoI CTYRAG 1 cut(s) 957
Sse9I AATT 9 cut(s) 93, 199, 300, 370, 525, 694, 876, 891, 1000
SseBI AGGCCT 1 cut(s) 282
SsiI CCGC 6 cut(s) 50, 396, 551, 612, 625, 849
SspMI CTAG 3 cut(s) 725, 800, 926
StuI AGGCCT 1 cut(s) 282
StyD4I CCNGG 4 cut(s) 316, 685, 715, 1032
StyI CCWWGG 1 cut(s) 733
TaaI ACNGT 4 cut(s) 60, 587, 871, 902
TaiI ACGT 5 cut(s) 314, 340, 363, 404, 654
TaqI TCGA 2 cut(s) 343, 1021
TasI AATT 9 cut(s) 93, 199, 300, 370, 525, 694, 876, 891, 1000
TauI GCSGC 2 cut(s) 53, 627
TfiI GAWTC 1 cut(s) 38
Tru1I TTAA 2 cut(s) 5, 222
Tru9I TTAA 2 cut(s) 5, 222
TscAI CASTG 4 cut(s) 213, 539, 925, 977
TseFI GTSAC 6 cut(s) 146, 208, 410, 571, 689, 929
TseI GCWGC 2 cut(s) 53, 609
Tsp45I GTSAC 6 cut(s) 146, 208, 410, 571, 689, 929
TspDTI ATGAA 1 cut(s) 192
TspRI CASTG 4 cut(s) 213, 539, 925, 977
Tth111I GACNNNGTC 1 cut(s) 362
VpaK11BI GGWCC 3 cut(s) 519, 816, 1036
XapI RAATTY 2 cut(s) 93, 300
XspI CTAG 3 cut(s) 725, 800, 926
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.