FvH4_6g40583

Nitrile-specifier protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0005031
Physical Location & Seq
Forward (+)
32059204 .. 32061526
2323 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g40583.t1

Sequence Viewer

Length: 990 bp
ATGGCTCTCAGGCAAGGCAAGTGGATCAAGCTTGATCAAGGGGCAACTGGGCCTGGAGCAAGAAGCTCACATGCCATCACCCAGGTTGGACAAAAAGCCTATTCCTTTGGTGGCGAATTCCAGCCACGTGTCCCAGTGGACAACAAGATGCACGTGTTCGACCTCAAAGAGCTGACGTGGTCTGCAATTTCCGGCACCGGTGATGTTCCACCGCCACGTGTCGGTGTGACAATGGCAACTATTGGAAAGACCATTTATATATTTGGTGGCAGGGACAATGAGCATAAGGAGCTCAATGAGCTTTACTCCTTTGACACATCCACAAACAATTGGACCCTAATTTCCAGTGGGGATGTTGGCCCCCCTCACCGGAGCTACCACTCTGTGACTACCGATGACCGCCATGTCTATATTTTTGGCGGCTGCGGAGTGGCCGGCCGCCTCAATGATCTATGGGCTTACGACGTTGTTGATCAAAAGTGGATCCAATATCCAAAACCAGGTGACAATTTAAGGGGAAGAGGTGGACCTGGACTCCTAGTGGCGCAAGGAAAGATATGGGTTGTGTATGGGTTTGCTGGAGAGGAAGCAGATGATGTACACACATTTGATCTAGCCCTTAAAGAGTGGATCCAAGTTGAGACAAGTGGGGAGAAACCAACTGCTCGAAGTGTATTTTCTACCGTTGCAATTGGGAAATACATAGTTGTATACGGTGGAGAAGTTGACCCCAGTGACTTGGGTCATATGGGTGCTGGGAAGTTTACTGCCCAAGCTTATGTATTGGACACAGAGACATTGTTGTGGAAGCAATTGGAGGATGGCTTGAACTCGAGTCATCATCCTGGACCTCGTGGGTGGTGTGCATTTACTGGTGGGGAACGAGATGGCCGAGTAGGGCTTTTGGTCTATGGTGGGAATTCGCCGAGTAATGATCGACTTGATGACATTTTCTTCTTCACTCCTCATTTCGAAGCAGATGAAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kelch_1 Kelch_3 Kelch_4

Protein Analysis

330

Amino Acids

36.05

Weight (kDa)

5.54

Isoelectric Point (pI)

20.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_ATRN-LZTR1 PF24981 3 - 82 6.7e-08 Attractin/LZTR1 beta-propeller
Beta-prop_FBX42 PF13415 7 - 111 1.4e-19 FBX42, beta-propeller domain
Beta-prop_Calicin PF13964 7 - 243 3.5e-09 Calicin, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 23 - 240 2e-70 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 48 - 130 2e-14 Attractin/LZTR1 beta-propeller
Kelch_HCF PF13854 66 - 163 4.2e-08 Host cell factor, Kelch-repeats domain
Kelch_FKB95 PF25210 70 - 188 3e-08 FKB95, Kelch-repeats domain
Kelch_1 PF01344 72 - 116 1.9e-10 Kelch motif
Beta-prop_ATRN-LZTR1 PF24981 85 - 164 9.4e-15 Attractin/LZTR1 beta-propeller
Beta-prop_FBX42 PF13415 96 - 196 2.1e-14 FBX42, beta-propeller domain
Kelch_1 PF01344 123 - 166 2.4e-10 Kelch motif
Beta-prop_FBX42 PF13415 124 - 321 1.6e-22 FBX42, beta-propeller domain
Kelch_HCF PF13854 184 - 290 4.1e-07 Host cell factor, Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 186 - 318 1e-12 Attractin/LZTR1 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 404
AccB1I GGYRCC 1 cut(s) 194
AccI GTMKAC 1 cut(s) 711
AciI CCGC 5 cut(s) 212, 400, 420, 426, 439
AclWI GGATC 5 cut(s) 32, 478, 491, 625, 638
AcoI YGGCCR 3 cut(s) 432, 436, 889
AcsI RAATTY 2 cut(s) 116, 919
AcvI CACGTG 3 cut(s) 128, 154, 218
AdeI CACNNNGTG 1 cut(s) 385
AfaI GTAC 1 cut(s) 600
AfiI CCNNNNNNNGG 3 cut(s) 221, 369, 500
AflIII ACRYGT 3 cut(s) 127, 153, 217
AgeI ACCGGT 1 cut(s) 197
AgsI TTSAA 1 cut(s) 829
AjiI CACGTC 1 cut(s) 177
AjnI CCWGG 5 cut(s) 52, 81, 499, 529, 844
AluBI AGCT 7 cut(s) 31, 66, 172, 292, 301, 375, 776
AluI AGCT 7 cut(s) 31, 66, 172, 292, 301, 375, 776
Alw21I GWGCWC 1 cut(s) 294
Alw26I GTCTC 2 cut(s) 635, 788
AlwI GGATC 5 cut(s) 32, 478, 491, 625, 638
Ama87I CYCGRG 1 cut(s) 832
AoxI GGCC 5 cut(s) 50, 358, 432, 436, 889
ApeKI GCWGC 1 cut(s) 423
ApoI RAATTY 2 cut(s) 116, 919
AsiGI ACCGGT 1 cut(s) 197
AspLEI GCGC 1 cut(s) 547
AspS9I GGNCC 5 cut(s) 50, 333, 359, 527, 848
AsuHPI GGTGA 4 cut(s) 70, 212, 359, 515
AsuII TTCGAA 1 cut(s) 972
AvaI CYCGRG 1 cut(s) 832
AvaII GGWCC 3 cut(s) 333, 527, 848
BamHI GGATCC 2 cut(s) 483, 630
BanI GGYRCC 1 cut(s) 194
BanII GRGCYC 1 cut(s) 294
BauI CACGAG 1 cut(s) 852
BbrPI CACGTG 3 cut(s) 128, 154, 218
Bbv12I GWGCWC 1 cut(s) 294
BbvI GCAGC 1 cut(s) 410
BccI CCATC 3 cut(s) 83, 815, 881
BciT130I CCWGG 5 cut(s) 54, 83, 501, 531, 846
BclI TGATCA 2 cut(s) 34, 472
BcoDI GTCTC 2 cut(s) 635, 788
BfaI CTAG 2 cut(s) 539, 614
BisI GCNGC 3 cut(s) 421, 424, 439
BlsI GCNGC 3 cut(s) 422, 425, 440
Bme1390I CCNGG 5 cut(s) 54, 83, 501, 531, 846
Bme18I GGWCC 3 cut(s) 333, 527, 848
BmeT110I CYCGRG 1 cut(s) 832
BmgBI CACGTC 1 cut(s) 177
BmgT120I GGNCC 5 cut(s) 50, 333, 359, 527, 848
BmiI GGNNCC 5 cut(s) 196, 335, 361, 485, 632
BmrFI CCNGG 5 cut(s) 54, 83, 501, 531, 846
BmrI ACTGGG 3 cut(s) 57, 128, 726
BmsI GCATC 1 cut(s) 138
BmuI ACTGGG 3 cut(s) 57, 128, 726
BoxI GACNNNNGTC 1 cut(s) 741
BplI GAGNNNNNCTC 2 cut(s) 290, 322
BpmI CTGGAG 2 cut(s) 75, 600
Bpu14I TTCGAA 1 cut(s) 972
BsaAI YACGTR 3 cut(s) 128, 154, 218
BsaJI CCNNGG 1 cut(s) 81
BsaWI WCCGGW 2 cut(s) 197, 369
BsaXI ACNNNNNCTCC 2 cut(s) 519, 549
Bsc4I CCNNNNNNNGG 3 cut(s) 221, 369, 500
Bse118I RCCGGY 2 cut(s) 197, 434
Bse1I ACTGG 5 cut(s) 52, 134, 345, 732, 877
BseBI CCWGG 5 cut(s) 54, 83, 501, 531, 846
BseDI CCNNGG 1 cut(s) 81
BseGI GGATG 4 cut(s) 317, 358, 826, 841
BseLI CCNNNNNNNGG 3 cut(s) 221, 369, 500
BseMII CTCAG 1 cut(s) 22
BseNI ACTGG 5 cut(s) 52, 134, 345, 732, 877
BseRI GAGGAG 1 cut(s) 954
BseX3I CGGCCG 1 cut(s) 436
BseXI GCAGC 1 cut(s) 410
BseYI CCCAGC 1 cut(s) 755
Bsh1285I CGRYCG 1 cut(s) 439
BshFI GGCC 5 cut(s) 52, 360, 434, 438, 891
BshNI GGYRCC 1 cut(s) 194
BshTI ACCGGT 1 cut(s) 197
BsiEI CGRYCG 1 cut(s) 439
BsiHKAI GWGCWC 1 cut(s) 294
BsiHKCI CYCGRG 1 cut(s) 832
BsiSI CCGG 4 cut(s) 192, 198, 370, 435
BslFI GGGAC 2 cut(s) 116, 287
BslI CCNNNNNNNGG 3 cut(s) 221, 369, 500
BsmAI GTCTC 2 cut(s) 635, 788
BsmFI GGGAC 2 cut(s) 116, 287
BsnI GGCC 5 cut(s) 52, 360, 434, 438, 891
BsoBI CYCGRG 1 cut(s) 832
Bsp119I TTCGAA 1 cut(s) 972
Bsp1286I GDGCHC 1 cut(s) 294
Bsp1407I TGTACA 1 cut(s) 598
Bsp143I GATC 8 cut(s) 24, 34, 448, 472, 483, 610, 630, 934
BspACI CCGC 5 cut(s) 212, 400, 420, 426, 439
BspANI GGCC 5 cut(s) 52, 360, 434, 438, 891
BspCNI CTCAG 1 cut(s) 21
BspLI GGNNCC 5 cut(s) 196, 335, 361, 485, 632
BspPI GGATC 5 cut(s) 32, 478, 491, 625, 638
BspT104I TTCGAA 1 cut(s) 972
BspT107I GGYRCC 1 cut(s) 194
BsrFI RCCGGY 2 cut(s) 197, 434
BsrGI TGTACA 1 cut(s) 598
BsrI ACTGG 5 cut(s) 52, 134, 345, 732, 877
BssAI RCCGGY 2 cut(s) 197, 434
BssECI CCNNGG 1 cut(s) 81
BssMI GATC 8 cut(s) 24, 34, 448, 472, 483, 610, 630, 934
BssNAI GTATAC 1 cut(s) 712
BssSI CACGAG 1 cut(s) 852
Bst1107I GTATAC 1 cut(s) 712
Bst2BI CACGAG 1 cut(s) 852
Bst2UI CCWGG 5 cut(s) 54, 83, 501, 531, 846
Bst4CI ACNGT 2 cut(s) 685, 716
Bst6I CTCTTC 1 cut(s) 514
BstAUI TGTACA 1 cut(s) 598
BstBAI YACGTR 3 cut(s) 128, 154, 218
BstBI TTCGAA 1 cut(s) 972
BstC8I GCNNGC 1 cut(s) 436
BstDEI CTNAG 1 cut(s) 8
BstF5I GGATG 4 cut(s) 317, 358, 826, 841
BstHHI GCGC 1 cut(s) 547
BstKTI GATC 8 cut(s) 27, 37, 451, 475, 486, 613, 633, 937
BstMAI GTCTC 2 cut(s) 635, 788
BstMBI GATC 8 cut(s) 24, 34, 448, 472, 483, 610, 630, 934
BstMCI CGRYCG 1 cut(s) 439
BstMWI GCNNNNNNNGC 2 cut(s) 289, 298
BstNI CCWGG 5 cut(s) 54, 83, 501, 531, 846
BstNSI RCATGY 1 cut(s) 74
BstPAI GACNNNNGTC 1 cut(s) 741
BstSCI CCNGG 5 cut(s) 52, 81, 499, 529, 844
BstV1I GCAGC 1 cut(s) 410
BstX2I RGATCY 2 cut(s) 483, 630
BstXI CCANNNNNNTGG 1 cut(s) 739
BstYI RGATCY 2 cut(s) 483, 630
BstZ17I GTATAC 1 cut(s) 712
BstZI CGGCCG 1 cut(s) 436
BsuRI GGCC 5 cut(s) 52, 360, 434, 438, 891
BtrI CACGTC 1 cut(s) 177
BtsCI GGATG 4 cut(s) 317, 358, 826, 841
BtsIMutI CAGTG 3 cut(s) 141, 352, 739
Cac8I GCNNGC 1 cut(s) 436
CfoI GCGC 1 cut(s) 547
Cfr10I RCCGGY 2 cut(s) 197, 434
Cfr13I GGNCC 5 cut(s) 50, 333, 359, 527, 848
CsiI ACCWGGT 1 cut(s) 499
Csp6I GTAC 1 cut(s) 599
CspAI ACCGGT 1 cut(s) 197
CspCI CAANNNNNGTGG 1 cut(s) 37
CviAII CATG 2 cut(s) 71, 404
CviQI GTAC 1 cut(s) 599
DdeI CTNAG 1 cut(s) 8
DpnI GATC 8 cut(s) 26, 36, 450, 474, 485, 612, 632, 936
DpnII GATC 8 cut(s) 24, 34, 448, 472, 483, 610, 630, 934
DraIII CACNNNGTG 1 cut(s) 385
DrdI GACNNNNNNGTC 1 cut(s) 404
DseDI GACNNNNNNGTC 1 cut(s) 404
EaeI YGGCCR 3 cut(s) 432, 436, 889
EagI CGGCCG 1 cut(s) 436
Eam1104I CTCTTC 1 cut(s) 514
EarI CTCTTC 1 cut(s) 514
Ecl136II GAGCTC 1 cut(s) 292
EclXI CGGCCG 1 cut(s) 436
Eco24I GRGCYC 1 cut(s) 294
Eco47I GGWCC 3 cut(s) 333, 527, 848
Eco52I CGGCCG 1 cut(s) 436
Eco53kI GAGCTC 1 cut(s) 292
Eco72I CACGTG 3 cut(s) 128, 154, 218
Eco88I CYCGRG 1 cut(s) 832
EcoICRI GAGCTC 1 cut(s) 292
EcoRI GAATTC 2 cut(s) 116, 919
EcoRII CCWGG 5 cut(s) 52, 81, 499, 529, 844
EcoT38I GRGCYC 1 cut(s) 294
FaeI CATG 2 cut(s) 74, 407
FaqI GGGAC 2 cut(s) 116, 287
FatI CATG 2 cut(s) 70, 403
FauNDI CATATG 1 cut(s) 747
FbaI TGATCA 2 cut(s) 34, 472
FblI GTMKAC 1 cut(s) 711
Fnu4HI GCNGC 3 cut(s) 421, 424, 439
FokI GGATG 4 cut(s) 304, 365, 828, 833
FriOI GRGCYC 1 cut(s) 294
FseI GGCCGGCC 1 cut(s) 438
Fsp4HI GCNGC 3 cut(s) 421, 424, 439
FspBI CTAG 2 cut(s) 539, 614
GlaI GCGC 1 cut(s) 546
GluI GCNGC 3 cut(s) 421, 424, 439
GsaI CCCAGC 1 cut(s) 759
GsuI CTGGAG 2 cut(s) 75, 600
HaeIII GGCC 5 cut(s) 52, 360, 434, 438, 891
HapII CCGG 4 cut(s) 192, 198, 370, 435
HhaI GCGC 1 cut(s) 547
Hin1II CATG 2 cut(s) 74, 407
Hin6I GCGC 1 cut(s) 545
HinP1I GCGC 1 cut(s) 545
HincII GTYRAC 1 cut(s) 727
HindII GTYRAC 1 cut(s) 727
HindIII AAGCTT 2 cut(s) 29, 774
HinfI GANTC 2 cut(s) 534, 835
HpaII CCGG 4 cut(s) 192, 198, 370, 435
HphI GGTGA 4 cut(s) 70, 212, 359, 515
Hpy166II GTNNAC 6 cut(s) 139, 527, 601, 712, 727, 765
Hpy8I GTNNAC 6 cut(s) 139, 527, 601, 712, 727, 765
Hpy99I CGWCG 1 cut(s) 467
HpyCH4III ACNGT 2 cut(s) 685, 716
HpyCH4IV ACGT 5 cut(s) 127, 153, 176, 217, 465
HpyCH4V TGCA 4 cut(s) 151, 185, 689, 866
HpyF10VI GCNNNNNNNGC 2 cut(s) 289, 298
HpyF3I CTNAG 1 cut(s) 8
HpySE526I ACGT 5 cut(s) 127, 153, 176, 217, 465
Hsp92II CATG 2 cut(s) 74, 407
HspAI GCGC 1 cut(s) 545
KroI GCCGGC 1 cut(s) 434
KroNI GCCGGC 1 cut(s) 436
Ksp22I TGATCA 2 cut(s) 34, 472
Kzo9I GATC 8 cut(s) 24, 34, 448, 472, 483, 610, 630, 934
LmnI GCTCC 3 cut(s) 56, 289, 372
Lsp1109I GCAGC 1 cut(s) 410
LweI GCATC 1 cut(s) 138
MabI ACCWGGT 1 cut(s) 499
MaeI CTAG 2 cut(s) 539, 614
MaeII ACGT 5 cut(s) 127, 153, 176, 217, 465
MaeIII GTNAC 4 cut(s) 226, 385, 503, 734
MalI GATC 8 cut(s) 26, 36, 450, 474, 485, 612, 632, 936
MboI GATC 8 cut(s) 24, 34, 448, 472, 483, 610, 630, 934
MboII GAAGA 3 cut(s) 531, 946, 949
MfeI CAATTG 3 cut(s) 328, 690, 812
MflI RGATCY 2 cut(s) 483, 630
MhlI GDGCHC 1 cut(s) 294
MluCI AATT 8 cut(s) 116, 186, 328, 339, 508, 690, 812, 919
MlyI GAGTC 2 cut(s) 528, 844
MmeI TCCRAC 1 cut(s) 67
MnlI CCTC 8 cut(s) 173, 375, 452, 515, 577, 811, 861, 975
MroNI GCCGGC 1 cut(s) 434
MseI TTAA 2 cut(s) 512, 621
MslI CAYNNNNRTG 2 cut(s) 750, 802
MspI CCGG 4 cut(s) 192, 198, 370, 435
MspR9I CCNGG 5 cut(s) 54, 83, 501, 531, 846
MunI CAATTG 3 cut(s) 328, 690, 812
MvaI CCWGG 5 cut(s) 54, 83, 501, 531, 846
MwoI GCNNNNNNNGC 2 cut(s) 289, 298
NaeI GCCGGC 1 cut(s) 436
NdeI CATATG 1 cut(s) 747
NdeII GATC 8 cut(s) 24, 34, 448, 472, 483, 610, 630, 934
NgoMIV GCCGGC 1 cut(s) 434
NlaIII CATG 2 cut(s) 74, 407
NlaIV GGNNCC 5 cut(s) 196, 335, 361, 485, 632
NmeAIII GCCGAG 2 cut(s) 917, 951
NmuCI GTSAC 4 cut(s) 226, 385, 503, 734
NspI RCATGY 1 cut(s) 74
NspV TTCGAA 1 cut(s) 972
PaeR7I CTCGAG 1 cut(s) 832
PcsI WCGNNNNNNNCGW 1 cut(s) 889
PdiI GCCGGC 1 cut(s) 436
PflFI GACNNNGTC 1 cut(s) 178
PfoI TCCNGGA 1 cut(s) 844
PinAI ACCGGT 1 cut(s) 197
PkrI GCNGC 3 cut(s) 422, 425, 440
PleI GAGTC 2 cut(s) 528, 843
PmaCI CACGTG 3 cut(s) 128, 154, 218
PmlI CACGTG 3 cut(s) 128, 154, 218
PpsI GAGTC 2 cut(s) 528, 843
Ppu21I YACGTR 3 cut(s) 128, 154, 218
PshAI GACNNNNGTC 1 cut(s) 741
Psp124BI GAGCTC 1 cut(s) 294
Psp6I CCWGG 5 cut(s) 52, 81, 499, 529, 844
PspCI CACGTG 3 cut(s) 128, 154, 218
PspFI CCCAGC 1 cut(s) 755
PspGI CCWGG 5 cut(s) 52, 81, 499, 529, 844
PspN4I GGNNCC 5 cut(s) 196, 335, 361, 485, 632
PspPI GGNCC 5 cut(s) 50, 333, 359, 527, 848
PspXI VCTCGAGB 1 cut(s) 832
PsuI RGATCY 2 cut(s) 483, 630
PsyI GACNNNGTC 1 cut(s) 178
RigI GGCCGGCC 1 cut(s) 438
RsaI GTAC 1 cut(s) 600
RsaNI GTAC 1 cut(s) 599
RseI CAYNNNNRTG 2 cut(s) 750, 802
SacI GAGCTC 1 cut(s) 294
SaqAI TTAA 2 cut(s) 512, 621
SatI GCNGC 3 cut(s) 421, 424, 439
Sau3AI GATC 8 cut(s) 24, 34, 448, 472, 483, 610, 630, 934
Sau96I GGNCC 5 cut(s) 50, 333, 359, 527, 848
SchI GAGTC 2 cut(s) 528, 844
ScrFI CCNGG 5 cut(s) 54, 83, 501, 531, 846
SduI GDGCHC 1 cut(s) 294
SexAI ACCWGGT 1 cut(s) 499
SfaNI GCATC 1 cut(s) 138
Sfr274I CTCGAG 1 cut(s) 832
SfuI TTCGAA 1 cut(s) 972
SgrAI CRCCGGYG 1 cut(s) 197
SinI GGWCC 3 cut(s) 333, 527, 848
SlaI CTCGAG 1 cut(s) 832
SmiMI CAYNNNNRTG 2 cut(s) 750, 802
SmlI CTYRAG 1 cut(s) 832
SmoI CTYRAG 1 cut(s) 832
Sse9I AATT 8 cut(s) 116, 186, 328, 339, 508, 690, 812, 919
SsiI CCGC 5 cut(s) 212, 400, 420, 426, 439
SspMI CTAG 2 cut(s) 539, 614
SstI GAGCTC 1 cut(s) 294
StyD4I CCNGG 5 cut(s) 52, 81, 499, 529, 844
TaaI ACNGT 2 cut(s) 685, 716
TaiI ACGT 5 cut(s) 130, 156, 179, 220, 468
TaqI TCGA 5 cut(s) 159, 667, 833, 937, 972
TasI AATT 8 cut(s) 116, 186, 328, 339, 508, 690, 812, 919
TatI WGTACW 1 cut(s) 598
TauI GCSGC 2 cut(s) 423, 441
Tru1I TTAA 2 cut(s) 512, 621
Tru9I TTAA 2 cut(s) 512, 621
TscAI CASTG 3 cut(s) 141, 352, 739
TseFI GTSAC 4 cut(s) 226, 385, 503, 734
TseI GCWGC 1 cut(s) 423
Tsp45I GTSAC 4 cut(s) 226, 385, 503, 734
TspRI CASTG 3 cut(s) 141, 352, 739
Tth111I GACNNNGTC 1 cut(s) 178
VpaK11BI GGWCC 3 cut(s) 333, 527, 848
XapI RAATTY 2 cut(s) 116, 919
XceI RCATGY 1 cut(s) 74
XhoI CTCGAG 1 cut(s) 832
XmiI GTMKAC 1 cut(s) 711
XspI CTAG 2 cut(s) 539, 614
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.