MD17G1117500.v1.1

Nitrile-specifier protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0005031
Physical Location & Seq
Reverse (-)
10180957 .. 10183336
2380 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1117500.v1.1.491

Sequence Viewer

Length: 987 bp
ATGTCTCTGGTTCAAGGCAAATGGATCAAGCTTGATCAAAAGGGGACTGGTCCTGGAGCAAGAAGCTCACATGCCATTACCCTAGTAGGACAGAAGGCCTATGTTTTCGGAGGCGAATTCACGCCACGTGTCCCGGTCGACAACAAGCTCCACGTGTTCAACATCAAGGAGCTGACGTGGTCTGTGATAGAAGGAACCGGGGATGTCCCGCCTCCACGTGTTGGTGTGACACTGGTAGCTGTTGGAGAAACTATTTATGTATTTGGAGGTAGGGATTATGAACACAATGAACTCAATGAGCTCTATTCCTTTGACACATCCACAAACAAGTGGACTCTAATTTCAAGTGGGGACACTGGGCCCCCTCACCGGAGTTACCACTCAGTCACCTCCGATGACCGCCATGTATACATATTTGGTGGATGTGGTGTCGCCGGTCGCCTCAATGATCTATGGGCCTACAATGTTGTTGATCAAAAGTGGATGCAATACCCAAATCCGGGTGACAGTTTGAAGGGGAGGGGTGGGCCGGGCCTGCTAGAGGTCCAAGGGAAAATATGGGTTGTGTATGGCTTTGCTGGAGAGGAAGTGGATGATGTACACATCTTTGATCCAGCCCAGGGTAAGTGGGCCCAAGTTGAGACAAGTGGCGAGAAACCAACAGCTCGGAGCGTATTTTCTACCGTAACAATTGGTAAATACATAATCATATACGGCGGTGAAGTGGACCCAAGTGACTTGGGCCACTTGGGTGCTGGGAAATTTGCTGCTGAGGTTTATGCATTGGACACTGAAACATTGCTGTGGAAGAGGTGGGATGATGGCCTGGGCTCGGACCATCATCCCGGGCCTCGCGGTTGGTGTGCGTTCGCCCGTGGTGAGTGGGAGGGGAAAGAAGGCCTTTTGGTGTATGGTGGGAATTCACCAACTAATGATCGACTTGGTGATATTCATTTCTTCACTCCTTATTTGGAAAATGGTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kelch_1 Kelch_3 Kelch_4

Protein Analysis

329

Amino Acids

35.99

Weight (kDa)

5.45

Isoelectric Point (pI)

20.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_ATRN-LZTR1 PF24981 4 - 82 7.1e-10 Attractin/LZTR1 beta-propeller
Beta-prop_FBX42 PF13415 7 - 111 6e-20 FBX42, beta-propeller domain
Beta-prop_Calicin PF13964 7 - 147 1.9e-08 Calicin, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 23 - 240 1.1e-70 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_HCF PF13854 50 - 141 5.8e-07 Host cell factor, Kelch-repeats domain
Kelch_FKB95 PF25210 70 - 237 1.1e-11 FKB95, Kelch-repeats domain
Kelch_1 PF01344 72 - 116 1.1e-11 Kelch motif
Beta-prop_ATRN-LZTR1 PF24981 85 - 165 4.8e-16 Attractin/LZTR1 beta-propeller
Kelch_1 PF01344 123 - 167 3.7e-10 Kelch motif
Beta-prop_FBX42 PF13415 124 - 321 1e-21 FBX42, beta-propeller domain
Kelch_1 PF01344 174 - 213 3.6e-06 Kelch motif
Beta-prop_ATRN-LZTR1 PF24981 186 - 313 4.4e-11 Attractin/LZTR1 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 221
AccI GTMKAC 2 cut(s) 138, 408
AccII CGCG 1 cut(s) 855
AciI CCGC 4 cut(s) 209, 400, 717, 855
AclWI GGATC 2 cut(s) 32, 605
AcsI RAATTY 3 cut(s) 116, 761, 919
AcvI CACGTG 3 cut(s) 128, 154, 218
AfaI GTAC 1 cut(s) 600
AfiI CCNNNNNNNGG 7 cut(s) 221, 369, 499, 500, 541, 620, 832
AflIII ACRYGT 3 cut(s) 127, 153, 217
AgsI TTSAA 4 cut(s) 14, 160, 345, 514
AjiI CACGTC 1 cut(s) 177
AjnI CCWGG 3 cut(s) 52, 618, 825
AleI CACNNNNGTG 1 cut(s) 750
AluBI AGCT 7 cut(s) 31, 66, 148, 172, 239, 301, 665
AluI AGCT 7 cut(s) 31, 66, 148, 172, 239, 301, 665
Alw21I GWGCWC 1 cut(s) 303
Alw26I GTCTC 2 cut(s) 9, 635
AlwI GGATC 2 cut(s) 32, 605
Ama87I CYCGRG 1 cut(s) 845
ApaI GGGCCC 2 cut(s) 363, 634
ApeKI GCWGC 1 cut(s) 767
ApoI RAATTY 3 cut(s) 116, 761, 919
AsuC2I CCSGG 6 cut(s) 134, 199, 501, 531, 846, 847
AsuHPI GGTGA 7 cut(s) 359, 379, 515, 731, 890, 915, 956
AvaI CYCGRG 1 cut(s) 845
AvaII GGWCC 4 cut(s) 50, 544, 727, 835
BaeGI GKGCMC 2 cut(s) 363, 634
BanII GRGCYC 4 cut(s) 303, 363, 634, 833
BbrPI CACGTG 3 cut(s) 128, 154, 218
Bbv12I GWGCWC 1 cut(s) 303
BbvCI CCTCAGC 1 cut(s) 771
BbvI GCAGC 1 cut(s) 754
BccI CCATC 2 cut(s) 815, 846
BceAI ACGGC 1 cut(s) 730
BciT130I CCWGG 3 cut(s) 54, 620, 827
BclI TGATCA 2 cut(s) 34, 472
BcnI CCSGG 6 cut(s) 134, 199, 501, 531, 846, 847
BcoDI GTCTC 2 cut(s) 9, 635
BfaI CTAG 2 cut(s) 83, 539
BisI GCNGC 1 cut(s) 768
BlsI GCNGC 1 cut(s) 769
Bme1390I CCNGG 9 cut(s) 54, 134, 199, 501, 531, 620, 827, 846, 847
Bme18I GGWCC 4 cut(s) 50, 544, 727, 835
BmeT110I CYCGRG 1 cut(s) 845
BmgBI CACGTC 1 cut(s) 177
BmiI GGNNCC 5 cut(s) 196, 361, 362, 632, 729
BmrFI CCNGG 9 cut(s) 54, 134, 199, 501, 531, 620, 827, 846, 847
BmrI ACTGGG 1 cut(s) 366
BmsI GCATC 1 cut(s) 474
BmuI ACTGGG 1 cut(s) 366
BpmI CTGGAG 2 cut(s) 75, 600
Bpu10I CCTNAGC 1 cut(s) 771
BpuMI CCSGG 6 cut(s) 134, 199, 501, 531, 846, 847
BsaAI YACGTR 3 cut(s) 128, 154, 218
BsaJI CCNNGG 7 cut(s) 198, 547, 618, 619, 826, 845, 874
BsaWI WCCGGW 1 cut(s) 369
BsaXI ACNNNNNCTCC 4 cut(s) 161, 191, 573, 603
Bsc4I CCNNNNNNNGG 7 cut(s) 221, 369, 499, 500, 541, 620, 832
Bse118I RCCGGY 1 cut(s) 434
Bse1I ACTGG 3 cut(s) 52, 237, 361
Bse3DI GCAATG 1 cut(s) 797
BseBI CCWGG 3 cut(s) 54, 620, 827
BseDI CCNNGG 7 cut(s) 198, 547, 618, 619, 826, 845, 874
BseGI GGATG 7 cut(s) 208, 317, 428, 489, 598, 823, 841
BseLI CCNNNNNNNGG 7 cut(s) 221, 369, 499, 500, 541, 620, 832
BseMI GCAATG 1 cut(s) 797
BseMII CTCAG 2 cut(s) 396, 762
BseNI ACTGG 3 cut(s) 52, 237, 361
BseSI GKGCMC 2 cut(s) 363, 634
BseXI GCAGC 1 cut(s) 754
BseYI CCCAGC 1 cut(s) 755
Bsh1236I CGCG 1 cut(s) 855
Bsh1285I CGRYCG 2 cut(s) 138, 439
BsiEI CGRYCG 2 cut(s) 138, 439
BsiHKAI GWGCWC 1 cut(s) 303
BsiHKCI CYCGRG 1 cut(s) 845
BsiSI CCGG 7 cut(s) 134, 198, 370, 435, 500, 530, 846
BslFI GGGAC 4 cut(s) 58, 116, 191, 365
BslI CCNNNNNNNGG 7 cut(s) 221, 369, 499, 500, 541, 620, 832
BsmAI GTCTC 2 cut(s) 9, 635
BsmFI GGGAC 4 cut(s) 58, 116, 191, 365
BsoBI CYCGRG 1 cut(s) 845
Bsp120I GGGCCC 2 cut(s) 359, 630
Bsp1286I GDGCHC 4 cut(s) 303, 363, 634, 833
Bsp1407I TGTACA 1 cut(s) 598
Bsp143I GATC 6 cut(s) 24, 34, 448, 472, 610, 934
BspACI CCGC 4 cut(s) 209, 400, 717, 855
BspCNI CTCAG 2 cut(s) 395, 763
BspFNI CGCG 1 cut(s) 855
BspLI GGNNCC 5 cut(s) 196, 361, 362, 632, 729
BspPI GGATC 2 cut(s) 32, 605
BsrDI GCAATG 1 cut(s) 797
BsrFI RCCGGY 1 cut(s) 434
BsrGI TGTACA 1 cut(s) 598
BsrI ACTGG 3 cut(s) 52, 237, 361
BssAI RCCGGY 1 cut(s) 434
BssECI CCNNGG 7 cut(s) 198, 547, 618, 619, 826, 845, 874
BssMI GATC 6 cut(s) 24, 34, 448, 472, 610, 934
BssNAI GTATAC 1 cut(s) 409
BssT1I CCWWGG 1 cut(s) 547
Bst1107I GTATAC 1 cut(s) 409
Bst2UI CCWGG 3 cut(s) 54, 620, 827
Bst4CI ACNGT 2 cut(s) 509, 685
Bst6I CTCTTC 1 cut(s) 803
BstAUI TGTACA 1 cut(s) 598
BstBAI YACGTR 3 cut(s) 128, 154, 218
BstC8I GCNNGC 1 cut(s) 536
BstDEI CTNAG 2 cut(s) 382, 771
BstDSI CCRYGG 1 cut(s) 874
BstENI CCTNNNNNAGG 1 cut(s) 539
BstF5I GGATG 7 cut(s) 208, 317, 428, 489, 598, 823, 841
BstFNI CGCG 1 cut(s) 855
BstKTI GATC 6 cut(s) 27, 37, 451, 475, 613, 937
BstMAI GTCTC 2 cut(s) 9, 635
BstMBI GATC 6 cut(s) 24, 34, 448, 472, 610, 934
BstMCI CGRYCG 2 cut(s) 138, 439
BstMWI GCNNNNNNNGC 1 cut(s) 535
BstNI CCWGG 3 cut(s) 54, 620, 827
BstNSI RCATGY 1 cut(s) 74
BstSCI CCNGG 9 cut(s) 52, 132, 197, 499, 529, 618, 825, 844, 845
BstSLI GKGCMC 2 cut(s) 363, 634
BstUI CGCG 1 cut(s) 855
BstV1I GCAGC 1 cut(s) 754
BstZ17I GTATAC 1 cut(s) 409
BtgI CCRYGG 1 cut(s) 874
BtrI CACGTC 1 cut(s) 177
BtsCI GGATG 7 cut(s) 208, 317, 428, 489, 598, 823, 841
BtsIMutI CAGTG 3 cut(s) 230, 354, 789
Cac8I GCNNGC 1 cut(s) 536
Cfr10I RCCGGY 1 cut(s) 434
Cfr9I CCCGGG 1 cut(s) 845
Csp6I GTAC 1 cut(s) 599
CviAII CATG 2 cut(s) 71, 404
CviQI GTAC 1 cut(s) 599
DdeI CTNAG 2 cut(s) 382, 771
DpnI GATC 6 cut(s) 26, 36, 450, 474, 612, 936
DpnII GATC 6 cut(s) 24, 34, 448, 472, 610, 934
Eam1104I CTCTTC 1 cut(s) 803
EarI CTCTTC 1 cut(s) 803
Ecl136II GAGCTC 1 cut(s) 301
Eco130I CCWWGG 1 cut(s) 547
Eco147I AGGCCT 2 cut(s) 98, 900
Eco24I GRGCYC 4 cut(s) 303, 363, 634, 833
Eco47I GGWCC 4 cut(s) 50, 544, 727, 835
Eco53kI GAGCTC 1 cut(s) 301
Eco72I CACGTG 3 cut(s) 128, 154, 218
Eco88I CYCGRG 1 cut(s) 845
EcoICRI GAGCTC 1 cut(s) 301
EcoNI CCTNNNNNAGG 1 cut(s) 539
EcoO109I RGGNCCY 1 cut(s) 360
EcoRI GAATTC 2 cut(s) 116, 919
EcoRII CCWGG 3 cut(s) 52, 618, 825
EcoT14I CCWWGG 1 cut(s) 547
EcoT22I ATGCAT 1 cut(s) 784
EcoT38I GRGCYC 4 cut(s) 303, 363, 634, 833
ErhI CCWWGG 1 cut(s) 547
FaeI CATG 2 cut(s) 74, 407
FalI AAGNNNNNCTT 2 cut(s) 885, 917
FaqI GGGAC 4 cut(s) 58, 116, 191, 365
FatI CATG 2 cut(s) 70, 403
FauI CCCGC 1 cut(s) 216
FbaI TGATCA 2 cut(s) 34, 472
FblI GTMKAC 2 cut(s) 138, 408
Fnu4HI GCNGC 1 cut(s) 768
FokI GGATG 7 cut(s) 215, 304, 435, 496, 605, 828, 830
FriOI GRGCYC 4 cut(s) 303, 363, 634, 833
Fsp4HI GCNGC 1 cut(s) 768
FspBI CTAG 2 cut(s) 83, 539
GluI GCNGC 1 cut(s) 768
GsaI CCCAGC 1 cut(s) 759
GsuI CTGGAG 2 cut(s) 75, 600
HapII CCGG 7 cut(s) 134, 198, 370, 435, 500, 530, 846
Hin1II CATG 2 cut(s) 74, 407
HincII GTYRAC 1 cut(s) 139
HindII GTYRAC 1 cut(s) 139
HindIII AAGCTT 1 cut(s) 29
HinfI GANTC 1 cut(s) 334
HpaII CCGG 7 cut(s) 134, 198, 370, 435, 500, 530, 846
HphI GGTGA 7 cut(s) 359, 379, 515, 731, 890, 915, 956
Hpy166II GTNNAC 5 cut(s) 139, 333, 409, 601, 727
Hpy188I TCNGA 4 cut(s) 110, 394, 669, 835
Hpy8I GTNNAC 5 cut(s) 139, 333, 409, 601, 727
HpyAV CCTTC 4 cut(s) 88, 185, 508, 890
HpyCH4III ACNGT 2 cut(s) 509, 685
HpyCH4IV ACGT 4 cut(s) 127, 153, 176, 217
HpyCH4V TGCA 2 cut(s) 487, 782
HpyF10VI GCNNNNNNNGC 1 cut(s) 535
HpyF3I CTNAG 2 cut(s) 382, 771
HpySE526I ACGT 4 cut(s) 127, 153, 176, 217
Hsp92II CATG 2 cut(s) 74, 407
Ksp22I TGATCA 2 cut(s) 34, 472
Kzo9I GATC 6 cut(s) 24, 34, 448, 472, 610, 934
LmnI GCTCC 4 cut(s) 56, 153, 169, 669
Lsp1109I GCAGC 1 cut(s) 754
LweI GCATC 1 cut(s) 474
MaeI CTAG 2 cut(s) 83, 539
MaeII ACGT 4 cut(s) 127, 153, 176, 217
MaeIII GTNAC 6 cut(s) 226, 374, 385, 503, 685, 734
MalI GATC 6 cut(s) 26, 36, 450, 474, 612, 936
MboI GATC 6 cut(s) 24, 34, 448, 472, 610, 934
MboII GAAGA 2 cut(s) 820, 949
MfeI CAATTG 1 cut(s) 690
MhlI GDGCHC 4 cut(s) 303, 363, 634, 833
MluCI AATT 5 cut(s) 116, 339, 690, 761, 919
MlyI GAGTC 1 cut(s) 328
MmeI TCCRAC 1 cut(s) 223
Mph1103I ATGCAT 1 cut(s) 784
MslI CAYNNNNRTG 2 cut(s) 750, 802
MspI CCGG 7 cut(s) 134, 198, 370, 435, 500, 530, 846
MspR9I CCNGG 9 cut(s) 54, 134, 199, 501, 531, 620, 827, 846, 847
MunI CAATTG 1 cut(s) 690
MvaI CCWGG 3 cut(s) 54, 620, 827
MvnI CGCG 1 cut(s) 855
MwoI GCNNNNNNNGC 1 cut(s) 535
NciI CCSGG 6 cut(s) 134, 199, 501, 531, 846, 847
NdeII GATC 6 cut(s) 24, 34, 448, 472, 610, 934
NlaIII CATG 2 cut(s) 74, 407
NlaIV GGNNCC 5 cut(s) 196, 361, 362, 632, 729
NmuCI GTSAC 4 cut(s) 226, 385, 503, 734
NsiI ATGCAT 1 cut(s) 784
NspI RCATGY 1 cut(s) 74
OliI CACNNNNGTG 1 cut(s) 750
PasI CCCWGGG 1 cut(s) 619
PceI AGGCCT 2 cut(s) 98, 900
PflFI GACNNNGTC 1 cut(s) 178
PflMI CCANNNNNTGG 1 cut(s) 221
PfoI TCCNGGA 1 cut(s) 52
PkrI GCNGC 1 cut(s) 769
PleI GAGTC 1 cut(s) 328
PmaCI CACGTG 3 cut(s) 128, 154, 218
PmlI CACGTG 3 cut(s) 128, 154, 218
PpsI GAGTC 1 cut(s) 328
Ppu21I YACGTR 3 cut(s) 128, 154, 218
Psp124BI GAGCTC 1 cut(s) 303
Psp6I CCWGG 3 cut(s) 52, 618, 825
PspCI CACGTG 3 cut(s) 128, 154, 218
PspFI CCCAGC 1 cut(s) 755
PspGI CCWGG 3 cut(s) 52, 618, 825
PspN4I GGNNCC 5 cut(s) 196, 361, 362, 632, 729
PspOMI GGGCCC 2 cut(s) 359, 630
PsyI GACNNNGTC 1 cut(s) 178
RsaI GTAC 1 cut(s) 600
RsaNI GTAC 1 cut(s) 599
RseI CAYNNNNRTG 2 cut(s) 750, 802
SacI GAGCTC 1 cut(s) 303
SalI GTCGAC 1 cut(s) 137
SatI GCNGC 1 cut(s) 768
Sau3AI GATC 6 cut(s) 24, 34, 448, 472, 610, 934
SchI GAGTC 1 cut(s) 328
ScrFI CCNGG 9 cut(s) 54, 134, 199, 501, 531, 620, 827, 846, 847
SduI GDGCHC 4 cut(s) 303, 363, 634, 833
SfaNI GCATC 1 cut(s) 474
SinI GGWCC 4 cut(s) 50, 544, 727, 835
SmaI CCCGGG 1 cut(s) 847
SmiMI CAYNNNNRTG 2 cut(s) 750, 802
Sse9I AATT 5 cut(s) 116, 339, 690, 761, 919
SseBI AGGCCT 2 cut(s) 98, 900
SsiI CCGC 4 cut(s) 209, 400, 717, 855
SspMI CTAG 2 cut(s) 83, 539
SstI GAGCTC 1 cut(s) 303
StuI AGGCCT 2 cut(s) 98, 900
StyD4I CCNGG 9 cut(s) 52, 132, 197, 499, 529, 618, 825, 844, 845
StyI CCWWGG 1 cut(s) 547
TaaI ACNGT 2 cut(s) 509, 685
TaiI ACGT 4 cut(s) 130, 156, 179, 220
TaqI TCGA 2 cut(s) 138, 937
TasI AATT 5 cut(s) 116, 339, 690, 761, 919
TatI WGTACW 1 cut(s) 598
TscAI CASTG 3 cut(s) 237, 361, 796
TseFI GTSAC 4 cut(s) 226, 385, 503, 734
TseI GCWGC 1 cut(s) 767
Tsp45I GTSAC 4 cut(s) 226, 385, 503, 734
TspDTI ATGAA 3 cut(s) 294, 303, 941
TspMI CCCGGG 1 cut(s) 845
TspRI CASTG 3 cut(s) 237, 361, 796
Tth111I GACNNNGTC 1 cut(s) 178
Van91I CCANNNNNTGG 1 cut(s) 221
VpaK11BI GGWCC 4 cut(s) 50, 544, 727, 835
XagI CCTNNNNNAGG 1 cut(s) 539
XapI RAATTY 3 cut(s) 116, 761, 919
XceI RCATGY 1 cut(s) 74
XcmI CCANNNNNNNNNTGG 1 cut(s) 752
XmaI CCCGGG 1 cut(s) 845
XmiI GTMKAC 2 cut(s) 138, 408
XspI CTAG 2 cut(s) 83, 539
Zsp2I ATGCAT 1 cut(s) 784
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.