Rw2G042570

Nitrile-specifier protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0005031
Physical Location & Seq
Forward (+)
68936516 .. 68937357
842 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G042570.1

Sequence Viewer

Length: 735 bp
ATGCACGTGTTCGACCTCAAAGAGCTGACGTGGTCTGCAATTTCCGGCACCGGCGATATCCCACCGCCACGCGTCGTGGACCCTAATTTCCAGTGGGGATGCAGCCCCCCTCACCGGAGCTACCACTCGGTGACTACCGACGACCGTCATGTGTATATTTTTGGGGGCTGCGGAGTGGCCGGCCGCCTCAATGATCTATGGGCTTACGATGTTGTTGATCAAAAGTGGATCCAATACCCAACACCGGGGGACAATTTGAAGGGAAGAGGTGGGCCTGGACTCCTAGTGTCCCAAGGAAAGATATGGGTTGTGTATGGCTTTGCTGGAGAGGAAGCAGATGATGTACACACATTTGATCTAGCCCATAAAGAGTGGACCCAAGTTGAGACAAGTGGGGAGAAACCAACAGCCCGAAGCGTATTTTCTACTGTTGCAATTGGGAAATACATAATTGTATACGGTGGAGAAGTTGACCCCAGTGATCTAGGTCACATGGGTGCCGGAAAGTTTACCGCTCAAGCTTACGCACTGGACACAGAGACATTGCTGTGGAAGCAATTGGATGATGGCTTGGAGTCGAGCCATCATCCTGGACCTCGCGGTTGGTGTGCATTTACGGGTGGGGAAAGAGATGGCCGAGTGGGACTTTTGGTCTATGGTGGGAATTCGCCGAGTAATGATCGGCTTGATGACATTTTCTTTTTCACTCCTTATTTCGAAGCAGATGAAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kelch_1 Kelch_3 Kelch_4

Protein Analysis

244

Amino Acids

26.89

Weight (kDa)

4.92

Isoelectric Point (pI)

20.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_KLHDC2_KLHL20_DRC7 PF24681 1 - 155 8.4e-39 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 4 - 79 1.6e-06 Attractin/LZTR1 beta-propeller
Kelch_1 PF01344 38 - 82 1.3e-10 Kelch motif
Beta-prop_FBX42 PF13415 39 - 236 1.4e-23 FBX42, beta-propeller domain
Kelch_HCF PF13854 99 - 205 1e-07 Host cell factor, Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 101 - 232 5.8e-14 Attractin/LZTR1 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 47, 497
AccBSI CCGCTC 1 cut(s) 515
AccI GTMKAC 1 cut(s) 456
AccII CGCG 2 cut(s) 72, 600
AciI CCGC 5 cut(s) 65, 171, 184, 513, 600
AclWI GGATC 2 cut(s) 223, 236
AcoI YGGCCR 3 cut(s) 177, 181, 634
AcsI RAATTY 1 cut(s) 664
AcvI CACGTG 1 cut(s) 7
AdeI CACNNNGTG 1 cut(s) 130
AfaI GTAC 1 cut(s) 345
AfiI CCNNNNNNNGG 3 cut(s) 114, 244, 245
AflIII ACRYGT 2 cut(s) 6, 70
AgsI TTSAA 1 cut(s) 259
AhdI GACNNNNNGTC 1 cut(s) 650
AjiI CACGTC 1 cut(s) 30
AjnI CCWGG 2 cut(s) 274, 589
AleI CACNNNNGTG 1 cut(s) 495
AluBI AGCT 3 cut(s) 25, 120, 521
AluI AGCT 3 cut(s) 25, 120, 521
Alw26I GTCTC 2 cut(s) 380, 533
AlwI GGATC 2 cut(s) 223, 236
AoxI GGCC 4 cut(s) 177, 181, 272, 634
ApeKI GCWGC 2 cut(s) 102, 168
ApoI RAATTY 1 cut(s) 664
AspS9I GGNCC 4 cut(s) 79, 272, 375, 593
AsuC2I CCSGG 1 cut(s) 246
AsuHPI GGTGA 2 cut(s) 104, 142
AsuII TTCGAA 1 cut(s) 717
AvaII GGWCC 3 cut(s) 79, 375, 593
BamHI GGATCC 1 cut(s) 228
BanI GGYRCC 2 cut(s) 47, 497
BbrPI CACGTG 1 cut(s) 7
BbvI GCAGC 2 cut(s) 114, 155
BccI CCATC 3 cut(s) 560, 591, 626
BciT130I CCWGG 2 cut(s) 276, 591
BclI TGATCA 1 cut(s) 217
BcnI CCSGG 1 cut(s) 246
BcoDI GTCTC 2 cut(s) 380, 533
BfaI CTAG 3 cut(s) 284, 359, 485
BisI GCNGC 3 cut(s) 103, 169, 184
BlsI GCNGC 3 cut(s) 104, 170, 185
Bme1390I CCNGG 3 cut(s) 246, 276, 591
Bme18I GGWCC 3 cut(s) 79, 375, 593
BmeRI GACNNNNNGTC 1 cut(s) 650
BmgBI CACGTC 1 cut(s) 30
BmgT120I GGNCC 4 cut(s) 79, 272, 375, 593
BmiI GGNNCC 5 cut(s) 49, 81, 230, 377, 499
BmrFI CCNGG 3 cut(s) 246, 276, 591
BmrI ACTGGG 1 cut(s) 471
BmsI GCATC 1 cut(s) 89
BmuI ACTGGG 1 cut(s) 471
BoxI GACNNNNGTC 1 cut(s) 144
BpmI CTGGAG 1 cut(s) 345
Bpu14I TTCGAA 1 cut(s) 717
BpuEI CTTGAG 1 cut(s) 501
BpuMI CCSGG 1 cut(s) 246
BsaAI YACGTR 1 cut(s) 7
BsaJI CCNNGG 2 cut(s) 245, 292
BsaWI WCCGGW 1 cut(s) 114
Bsc4I CCNNNNNNNGG 3 cut(s) 114, 244, 245
Bse118I RCCGGY 2 cut(s) 50, 179
Bse1I ACTGG 3 cut(s) 91, 477, 534
Bse3DI GCAATG 1 cut(s) 542
BseBI CCWGG 2 cut(s) 276, 591
BseDI CCNNGG 2 cut(s) 245, 292
BseGI GGATG 3 cut(s) 104, 568, 586
BseLI CCNNNNNNNGG 3 cut(s) 114, 244, 245
BseMI GCAATG 1 cut(s) 542
BseNI ACTGG 3 cut(s) 91, 477, 534
BseX3I CGGCCG 1 cut(s) 181
BseXI GCAGC 2 cut(s) 114, 155
Bsh1236I CGCG 2 cut(s) 72, 600
Bsh1285I CGRYCG 2 cut(s) 145, 184
BshFI GGCC 4 cut(s) 179, 183, 274, 636
BshNI GGYRCC 2 cut(s) 47, 497
BsiEI CGRYCG 2 cut(s) 145, 184
BsiSI CCGG 6 cut(s) 45, 51, 115, 180, 245, 501
BslFI GGGAC 3 cut(s) 263, 274, 657
BslI CCNNNNNNNGG 3 cut(s) 114, 244, 245
BsmAI GTCTC 2 cut(s) 380, 533
BsmFI GGGAC 3 cut(s) 263, 274, 657
BsnI GGCC 4 cut(s) 179, 183, 274, 636
Bsp119I TTCGAA 1 cut(s) 717
Bsp1407I TGTACA 1 cut(s) 343
Bsp143I GATC 6 cut(s) 193, 217, 228, 355, 481, 679
BspACI CCGC 5 cut(s) 65, 171, 184, 513, 600
BspANI GGCC 4 cut(s) 179, 183, 274, 636
BspFNI CGCG 2 cut(s) 72, 600
BspLI GGNNCC 5 cut(s) 49, 81, 230, 377, 499
BspPI GGATC 2 cut(s) 223, 236
BspT104I TTCGAA 1 cut(s) 717
BspT107I GGYRCC 2 cut(s) 47, 497
BsrBI CCGCTC 1 cut(s) 515
BsrDI GCAATG 1 cut(s) 542
BsrFI RCCGGY 2 cut(s) 50, 179
BsrGI TGTACA 1 cut(s) 343
BsrI ACTGG 3 cut(s) 91, 477, 534
BssAI RCCGGY 2 cut(s) 50, 179
BssECI CCNNGG 2 cut(s) 245, 292
BssMI GATC 6 cut(s) 193, 217, 228, 355, 481, 679
BssNAI GTATAC 1 cut(s) 457
BssT1I CCWWGG 1 cut(s) 292
Bst1107I GTATAC 1 cut(s) 457
Bst2UI CCWGG 2 cut(s) 276, 591
Bst4CI ACNGT 3 cut(s) 146, 430, 461
Bst6I CTCTTC 1 cut(s) 259
BstAUI TGTACA 1 cut(s) 343
BstBAI YACGTR 1 cut(s) 7
BstBI TTCGAA 1 cut(s) 717
BstC8I GCNNGC 1 cut(s) 181
BstF5I GGATG 3 cut(s) 104, 568, 586
BstFNI CGCG 2 cut(s) 72, 600
BstKTI GATC 6 cut(s) 196, 220, 231, 358, 484, 682
BstMAI GTCTC 2 cut(s) 380, 533
BstMBI GATC 6 cut(s) 193, 217, 228, 355, 481, 679
BstMCI CGRYCG 2 cut(s) 145, 184
BstMWI GCNNNNNNNGC 1 cut(s) 553
BstNI CCWGG 2 cut(s) 276, 591
BstPAI GACNNNNGTC 1 cut(s) 144
BstSCI CCNGG 3 cut(s) 244, 274, 589
BstUI CGCG 2 cut(s) 72, 600
BstV1I GCAGC 2 cut(s) 114, 155
BstX2I RGATCY 1 cut(s) 228
BstXI CCANNNNNNTGG 1 cut(s) 590
BstYI RGATCY 1 cut(s) 228
BstZ17I GTATAC 1 cut(s) 457
BstZI CGGCCG 1 cut(s) 181
BsuRI GGCC 4 cut(s) 179, 183, 274, 636
BtrI CACGTC 1 cut(s) 30
BtsCI GGATG 3 cut(s) 104, 568, 586
BtsIMutI CAGTG 3 cut(s) 98, 484, 527
Cac8I GCNNGC 1 cut(s) 181
Cfr10I RCCGGY 2 cut(s) 50, 179
Cfr13I GGNCC 4 cut(s) 79, 272, 375, 593
CseI GACGC 1 cut(s) 61
Csp6I GTAC 1 cut(s) 344
CviAII CATG 2 cut(s) 149, 493
CviQI GTAC 1 cut(s) 344
DpnI GATC 6 cut(s) 195, 219, 230, 357, 483, 681
DpnII GATC 6 cut(s) 193, 217, 228, 355, 481, 679
DraIII CACNNNGTG 1 cut(s) 130
DriI GACNNNNNGTC 1 cut(s) 650
EaeI YGGCCR 3 cut(s) 177, 181, 634
EagI CGGCCG 1 cut(s) 181
Eam1104I CTCTTC 1 cut(s) 259
Eam1105I GACNNNNNGTC 1 cut(s) 650
EarI CTCTTC 1 cut(s) 259
EclXI CGGCCG 1 cut(s) 181
Eco130I CCWWGG 1 cut(s) 292
Eco32I GATATC 1 cut(s) 58
Eco47I GGWCC 3 cut(s) 79, 375, 593
Eco52I CGGCCG 1 cut(s) 181
Eco72I CACGTG 1 cut(s) 7
EcoRI GAATTC 1 cut(s) 664
EcoRII CCWGG 2 cut(s) 274, 589
EcoRV GATATC 1 cut(s) 58
EcoT14I CCWWGG 1 cut(s) 292
ErhI CCWWGG 1 cut(s) 292
FaeI CATG 2 cut(s) 152, 496
FaqI GGGAC 3 cut(s) 263, 274, 657
FatI CATG 2 cut(s) 148, 492
FbaI TGATCA 1 cut(s) 217
FblI GTMKAC 1 cut(s) 456
Fnu4HI GCNGC 3 cut(s) 103, 169, 184
FokI GGATG 3 cut(s) 111, 573, 575
FseI GGCCGGCC 1 cut(s) 183
Fsp4HI GCNGC 3 cut(s) 103, 169, 184
FspBI CTAG 3 cut(s) 284, 359, 485
GluI GCNGC 3 cut(s) 103, 169, 184
GsuI CTGGAG 1 cut(s) 345
HaeIII GGCC 4 cut(s) 179, 183, 274, 636
HapII CCGG 6 cut(s) 45, 51, 115, 180, 245, 501
HgaI GACGC 1 cut(s) 61
Hin1II CATG 2 cut(s) 152, 496
HincII GTYRAC 1 cut(s) 472
HindII GTYRAC 1 cut(s) 472
HindIII AAGCTT 1 cut(s) 519
HinfI GANTC 2 cut(s) 279, 575
HpaII CCGG 6 cut(s) 45, 51, 115, 180, 245, 501
HphI GGTGA 2 cut(s) 104, 142
Hpy166II GTNNAC 6 cut(s) 79, 346, 375, 457, 472, 510
Hpy8I GTNNAC 6 cut(s) 79, 346, 375, 457, 472, 510
Hpy99I CGWCG 2 cut(s) 77, 143
HpyAV CCTTC 1 cut(s) 253
HpyCH4III ACNGT 3 cut(s) 146, 430, 461
HpyCH4IV ACGT 2 cut(s) 6, 29
HpyCH4V TGCA 5 cut(s) 4, 38, 102, 434, 611
HpyF10VI GCNNNNNNNGC 1 cut(s) 553
HpySE526I ACGT 2 cut(s) 6, 29
Hsp92II CATG 2 cut(s) 152, 496
KroI GCCGGC 1 cut(s) 179
KroNI GCCGGC 1 cut(s) 181
Ksp22I TGATCA 1 cut(s) 217
Kzo9I GATC 6 cut(s) 193, 217, 228, 355, 481, 679
LmnI GCTCC 1 cut(s) 117
Lsp1109I GCAGC 2 cut(s) 114, 155
LweI GCATC 1 cut(s) 89
MaeI CTAG 3 cut(s) 284, 359, 485
MaeII ACGT 2 cut(s) 6, 29
MaeIII GTNAC 2 cut(s) 130, 488
MalI GATC 6 cut(s) 195, 219, 230, 357, 483, 681
MbiI CCGCTC 1 cut(s) 515
MboI GATC 6 cut(s) 193, 217, 228, 355, 481, 679
MboII GAAGA 1 cut(s) 276
MfeI CAATTG 2 cut(s) 435, 557
MflI RGATCY 1 cut(s) 228
MluCI AATT 7 cut(s) 39, 85, 253, 435, 450, 557, 664
MluI ACGCGT 1 cut(s) 70
MlyI GAGTC 2 cut(s) 273, 584
MnlI CCTC 6 cut(s) 26, 120, 197, 260, 322, 606
MroNI GCCGGC 1 cut(s) 179
MslI CAYNNNNRTG 2 cut(s) 495, 547
MspI CCGG 6 cut(s) 45, 51, 115, 180, 245, 501
MspR9I CCNGG 3 cut(s) 246, 276, 591
MunI CAATTG 2 cut(s) 435, 557
MvaI CCWGG 2 cut(s) 276, 591
MvnI CGCG 2 cut(s) 72, 600
MwoI GCNNNNNNNGC 1 cut(s) 553
NaeI GCCGGC 1 cut(s) 181
NciI CCSGG 1 cut(s) 246
NdeII GATC 6 cut(s) 193, 217, 228, 355, 481, 679
NgoMIV GCCGGC 1 cut(s) 179
NlaIII CATG 2 cut(s) 152, 496
NlaIV GGNNCC 5 cut(s) 49, 81, 230, 377, 499
NmeAIII GCCGAG 2 cut(s) 662, 696
NmuCI GTSAC 2 cut(s) 130, 488
NspV TTCGAA 1 cut(s) 717
OliI CACNNNNGTG 1 cut(s) 495
PdiI GCCGGC 1 cut(s) 181
PflFI GACNNNGTC 1 cut(s) 31
PfoI TCCNGGA 1 cut(s) 589
PkrI GCNGC 3 cut(s) 104, 170, 185
PleI GAGTC 2 cut(s) 273, 583
PmaCI CACGTG 1 cut(s) 7
PmlI CACGTG 1 cut(s) 7
PpsI GAGTC 2 cut(s) 273, 583
Ppu21I YACGTR 1 cut(s) 7
PshAI GACNNNNGTC 1 cut(s) 144
Psp6I CCWGG 2 cut(s) 274, 589
PspCI CACGTG 1 cut(s) 7
PspGI CCWGG 2 cut(s) 274, 589
PspN4I GGNNCC 5 cut(s) 49, 81, 230, 377, 499
PspPI GGNCC 4 cut(s) 79, 272, 375, 593
PsuI RGATCY 1 cut(s) 228
PsyI GACNNNGTC 1 cut(s) 31
RigI GGCCGGCC 1 cut(s) 183
RsaI GTAC 1 cut(s) 345
RsaNI GTAC 1 cut(s) 344
RseI CAYNNNNRTG 2 cut(s) 495, 547
SatI GCNGC 3 cut(s) 103, 169, 184
Sau3AI GATC 6 cut(s) 193, 217, 228, 355, 481, 679
Sau96I GGNCC 4 cut(s) 79, 272, 375, 593
SchI GAGTC 2 cut(s) 273, 584
ScrFI CCNGG 3 cut(s) 246, 276, 591
SetI ASST 9 cut(s) 9, 18, 27, 32, 122, 271, 490, 523, 598
SfaNI GCATC 1 cut(s) 89
SfuI TTCGAA 1 cut(s) 717
SgrAI CRCCGGYG 1 cut(s) 50
SinI GGWCC 3 cut(s) 79, 375, 593
SmiMI CAYNNNNRTG 2 cut(s) 495, 547
SmlI CTYRAG 1 cut(s) 516
SmoI CTYRAG 1 cut(s) 516
Sse9I AATT 7 cut(s) 39, 85, 253, 435, 450, 557, 664
SsiI CCGC 5 cut(s) 65, 171, 184, 513, 600
SspMI CTAG 3 cut(s) 284, 359, 485
StyD4I CCNGG 3 cut(s) 244, 274, 589
StyI CCWWGG 1 cut(s) 292
TaaI ACNGT 3 cut(s) 146, 430, 461
TaiI ACGT 2 cut(s) 9, 32
TaqI TCGA 3 cut(s) 12, 578, 717
TasI AATT 7 cut(s) 39, 85, 253, 435, 450, 557, 664
TatI WGTACW 1 cut(s) 343
TauI GCSGC 1 cut(s) 186
TscAI CASTG 3 cut(s) 98, 484, 534
TseFI GTSAC 2 cut(s) 130, 488
TseI GCWGC 2 cut(s) 102, 168
Tsp45I GTSAC 2 cut(s) 130, 488
TspRI CASTG 3 cut(s) 98, 484, 534
Tth111I GACNNNGTC 1 cut(s) 31
VpaK11BI GGWCC 3 cut(s) 79, 375, 593
XapI RAATTY 1 cut(s) 664
XmiI GTMKAC 1 cut(s) 456
XspI CTAG 3 cut(s) 284, 359, 485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.