MD09G1127800.v1.1

Nitrile-specifier protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0005031
Physical Location & Seq
Reverse (-)
9839574 .. 9840568
995 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1127800.v1.1.491

Sequence Viewer

Length: 939 bp
ATGCATCAAAACCCCGAAAGTTTCTTCTGCATTTGTCCAATTAGTAATACAGTATCCTTGCGTCAGCTTGATCAAAAGGGGACTGGTCCTGGAGCTAGAAGCTCACATGCCATTACCCTAGTAGGACAGAAGGCCTATGTTTTCGAAGGCGAATTCACGCCACGTGTCCCGGTAGACAACAAGCTCCATGTGTTCGACGTCAAGGAGCAGACGTGGTATGTGATTGAAGGAACCAGGGATGTCCCACCGCCATGTGTTGGTGTGACACTAGTAGCTGTTGGAGAAATTATTTATGTATTTGGAGGTAGGGATTATGAACATAATGAGCTCTATTCCTTTGACACATCCACACACAAGTGGACTCTAATTTCAAGTGGGGACACCGGGCCCCCTCACCGGAGTTACCACTCAGTCACCTCCGATGACCACCACGTATACATCTTCGGCGGATGTGGTGTTGCCGGCCGCTCAATGATCTATGGGCCTACAATGGGTGGGGCGGGCCTGCTAGAGGTCCAAGGAAAAATATGGGTTGTGTATGGCTTCGCTGGAGAGGAAGTGGATGATGTACACATCCTTGATCCAGCCCATGGTGAGTGGGCCCAAGTTGAGACAAATGGCAAGAAACCAACAGCTCGAAGTGTATTTTCTACCGTAGCAATCAGTAAATACATAATCATATACGGCGGGGAAGTGGACCCTAGTGACTTGGGCCACTTGGGTGTTGGAAAATTTACAGCCGAGGTTTATGCATTGGACACAGAAACATTGTTGTGGAAGAAGTGGGATGATGGTCTAGGCTTAGGCCATCATCCTGGCCCTCGCGGATGGTGTGAGTTTGCCCGCGGGCAATGGAAAGGGAAAGAGGGCCATTTGGTGTATGATGGGAATTCACCAACTAATGATCGACTTGGTGATATTCATTTCTTCACTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kelch_1 Kelch_3 Kelch_4

Protein Analysis

313

Amino Acids

34.31

Weight (kDa)

5.6

Isoelectric Point (pI)

23.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_ATRN-LZTR1 PF24981 20 - 102 8.1e-08 Attractin/LZTR1 beta-propeller
Beta-prop_FBX42 PF13415 22 - 154 2.7e-22 FBX42, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 35 - 156 3.4e-34 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_HCF PF13854 60 - 151 3.3e-08 Host cell factor, Kelch-repeats domain
Kelch_1 PF01344 84 - 125 5.5e-09 Kelch motif
Beta-prop_ATRN-LZTR1 PF24981 97 - 151 6.3e-08 Attractin/LZTR1 beta-propeller
Kelch_KLHDC2_KLHL20_DRC7 PF24681 168 - 230 2.5e-12 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 176 - 265 2.2e-08 Attractin/LZTR1 beta-propeller
Beta-prop_FBX42 PF13415 186 - 261 2.9e-08 FBX42, beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 201
AccB7I CCANNNNNTGG 2 cut(s) 257, 590
AccBSI CCGCTC 1 cut(s) 468
AccI GTMKAC 2 cut(s) 174, 435
AccII CGCG 2 cut(s) 825, 846
AciI CCGC 8 cut(s) 248, 447, 466, 500, 687, 825, 844, 846
AclWI GGATC 1 cut(s) 575
AcoI YGGCCR 1 cut(s) 463
AcsI RAATTY 3 cut(s) 152, 731, 889
AcvI CACGTG 1 cut(s) 164
AcyI GRCGYC 1 cut(s) 198
AfaI GTAC 1 cut(s) 570
AfiI CCNNNNNNNGG 5 cut(s) 257, 396, 491, 511, 590
AflIII ACRYGT 1 cut(s) 163
AgsI TTSAA 2 cut(s) 227, 372
AhlI ACTAGT 1 cut(s) 268
AjiI CACGTC 1 cut(s) 213
AjnI CCWGG 3 cut(s) 88, 233, 814
AleI CACNNNNGTG 2 cut(s) 355, 720
AluBI AGCT 7 cut(s) 67, 95, 102, 184, 275, 328, 635
AluI AGCT 7 cut(s) 67, 95, 102, 184, 275, 328, 635
Alw21I GWGCWC 1 cut(s) 330
Alw26I GTCTC 1 cut(s) 605
AlwI GGATC 1 cut(s) 575
ApaI GGGCCC 2 cut(s) 390, 604
ApoI RAATTY 3 cut(s) 152, 731, 889
AsuC2I CCSGG 2 cut(s) 170, 385
AsuHPI GGTGA 5 cut(s) 386, 406, 605, 885, 926
AsuII TTCGAA 1 cut(s) 144
AvaII GGWCC 3 cut(s) 86, 514, 697
BaeGI GKGCMC 2 cut(s) 390, 604
BanII GRGCYC 3 cut(s) 330, 390, 604
BbrPI CACGTG 1 cut(s) 164
Bbv12I GWGCWC 1 cut(s) 330
BccI CCATC 4 cut(s) 785, 816, 822, 878
BceAI ACGGC 1 cut(s) 700
BcgI CGANNNNNNTGC 2 cut(s) 731, 765
BciT130I CCWGG 3 cut(s) 90, 235, 816
BciVI GTATCC 1 cut(s) 64
BclI TGATCA 1 cut(s) 70
BcnI CCSGG 2 cut(s) 170, 385
BcoDI GTCTC 1 cut(s) 605
BcuI ACTAGT 1 cut(s) 268
BfaI CTAG 6 cut(s) 96, 119, 269, 509, 702, 797
BfuI GTATCC 1 cut(s) 64
BisI GCNGC 1 cut(s) 466
BlsI GCNGC 1 cut(s) 467
Bme1390I CCNGG 5 cut(s) 90, 170, 235, 385, 816
Bme18I GGWCC 3 cut(s) 86, 514, 697
BmgBI CACGTC 1 cut(s) 213
BmiI GGNNCC 5 cut(s) 232, 388, 389, 602, 699
BmrFI CCNGG 5 cut(s) 90, 170, 235, 385, 816
BmsI GCATC 1 cut(s) 13
BpmI CTGGAG 2 cut(s) 111, 570
Bpu10I CCTNAGC 1 cut(s) 802
Bpu14I TTCGAA 1 cut(s) 144
BpuMI CCSGG 2 cut(s) 170, 385
BsaAI YACGTR 2 cut(s) 164, 433
BsaHI GRCGYC 1 cut(s) 198
BsaJI CCNNGG 5 cut(s) 234, 517, 589, 741, 844
BsaWI WCCGGW 1 cut(s) 396
BsaXI ACNNNNNCTCC 4 cut(s) 197, 227, 543, 573
Bsc4I CCNNNNNNNGG 5 cut(s) 257, 396, 491, 511, 590
Bse118I RCCGGY 1 cut(s) 461
Bse1I ACTGG 1 cut(s) 88
Bse3DI GCAATG 1 cut(s) 857
BseBI CCWGG 3 cut(s) 90, 235, 816
BseDI CCNNGG 5 cut(s) 234, 517, 589, 741, 844
BseGI GGATG 8 cut(s) 244, 344, 455, 568, 573, 793, 811, 833
BseLI CCNNNNNNNGG 5 cut(s) 257, 396, 491, 511, 590
BseMI GCAATG 1 cut(s) 857
BseMII CTCAG 1 cut(s) 423
BseNI ACTGG 1 cut(s) 88
BseSI GKGCMC 2 cut(s) 390, 604
BseX3I CGGCCG 1 cut(s) 463
Bsh1236I CGCG 2 cut(s) 825, 846
Bsh1285I CGRYCG 1 cut(s) 466
BsiEI CGRYCG 1 cut(s) 466
BsiHKAI GWGCWC 1 cut(s) 330
BsiSI CCGG 4 cut(s) 170, 384, 397, 462
BslFI GGGAC 4 cut(s) 94, 152, 227, 392
BslI CCNNNNNNNGG 5 cut(s) 257, 396, 491, 511, 590
BsmAI GTCTC 1 cut(s) 605
BsmFI GGGAC 4 cut(s) 94, 152, 227, 392
Bsp119I TTCGAA 1 cut(s) 144
Bsp120I GGGCCC 2 cut(s) 386, 600
Bsp1286I GDGCHC 3 cut(s) 330, 390, 604
Bsp1407I TGTACA 1 cut(s) 568
Bsp143I GATC 4 cut(s) 70, 474, 580, 904
Bsp19I CCATGG 1 cut(s) 589
BspACI CCGC 8 cut(s) 248, 447, 466, 500, 687, 825, 844, 846
BspCNI CTCAG 1 cut(s) 422
BspFNI CGCG 2 cut(s) 825, 846
BspLI GGNNCC 5 cut(s) 232, 388, 389, 602, 699
BspPI GGATC 1 cut(s) 575
BspT104I TTCGAA 1 cut(s) 144
BsrBI CCGCTC 1 cut(s) 468
BsrDI GCAATG 1 cut(s) 857
BsrFI RCCGGY 1 cut(s) 461
BsrGI TGTACA 1 cut(s) 568
BsrI ACTGG 1 cut(s) 88
BssAI RCCGGY 1 cut(s) 461
BssECI CCNNGG 5 cut(s) 234, 517, 589, 741, 844
BssMI GATC 4 cut(s) 70, 474, 580, 904
BssNAI GTATAC 1 cut(s) 436
BssNI GRCGYC 1 cut(s) 198
BssT1I CCWWGG 2 cut(s) 517, 589
Bst1107I GTATAC 1 cut(s) 436
Bst2UI CCWGG 3 cut(s) 90, 235, 816
Bst4CI ACNGT 2 cut(s) 52, 655
BstACI GRCGYC 1 cut(s) 198
BstAUI TGTACA 1 cut(s) 568
BstBAI YACGTR 2 cut(s) 164, 433
BstBI TTCGAA 1 cut(s) 144
BstC8I GCNNGC 5 cut(s) 463, 502, 506, 844, 848
BstDEI CTNAG 2 cut(s) 409, 802
BstDSI CCRYGG 2 cut(s) 589, 844
BstENI CCTNNNNNAGG 1 cut(s) 509
BstF5I GGATG 8 cut(s) 244, 344, 455, 568, 573, 793, 811, 833
BstFNI CGCG 2 cut(s) 825, 846
BstKTI GATC 4 cut(s) 73, 477, 583, 907
BstMAI GTCTC 1 cut(s) 605
BstMBI GATC 4 cut(s) 70, 474, 580, 904
BstMCI CGRYCG 1 cut(s) 466
BstNI CCWGG 3 cut(s) 90, 235, 816
BstNSI RCATGY 1 cut(s) 110
BstSCI CCNGG 5 cut(s) 88, 168, 233, 383, 814
BstSLI GKGCMC 2 cut(s) 390, 604
BstUI CGCG 2 cut(s) 825, 846
BstXI CCANNNNNNTGG 1 cut(s) 815
BstZ17I GTATAC 1 cut(s) 436
BstZI CGGCCG 1 cut(s) 463
BsuI GTATCC 1 cut(s) 64
BtgI CCRYGG 2 cut(s) 589, 844
BtrI CACGTC 1 cut(s) 213
BtsCI GGATG 8 cut(s) 244, 344, 455, 568, 573, 793, 811, 833
Cac8I GCNNGC 5 cut(s) 463, 502, 506, 844, 848
Cfr10I RCCGGY 1 cut(s) 461
Cfr42I CCGCGG 1 cut(s) 847
CseI GACGC 1 cut(s) 50
Csp6I GTAC 1 cut(s) 569
CviAII CATG 4 cut(s) 107, 188, 252, 590
CviQI GTAC 1 cut(s) 569
DdeI CTNAG 2 cut(s) 409, 802
DpnI GATC 4 cut(s) 72, 476, 582, 906
DpnII GATC 4 cut(s) 70, 474, 580, 904
EaeI YGGCCR 1 cut(s) 463
EagI CGGCCG 1 cut(s) 463
EciI GGCGGA 1 cut(s) 462
Ecl136II GAGCTC 1 cut(s) 328
EclXI CGGCCG 1 cut(s) 463
Eco130I CCWWGG 2 cut(s) 517, 589
Eco147I AGGCCT 1 cut(s) 134
Eco24I GRGCYC 3 cut(s) 330, 390, 604
Eco47I GGWCC 3 cut(s) 86, 514, 697
Eco52I CGGCCG 1 cut(s) 463
Eco53kI GAGCTC 1 cut(s) 328
Eco72I CACGTG 1 cut(s) 164
EcoICRI GAGCTC 1 cut(s) 328
EcoNI CCTNNNNNAGG 1 cut(s) 509
EcoO109I RGGNCCY 1 cut(s) 387
EcoRI GAATTC 2 cut(s) 152, 889
EcoRII CCWGG 3 cut(s) 88, 233, 814
EcoT14I CCWWGG 2 cut(s) 517, 589
EcoT22I ATGCAT 2 cut(s) 6, 754
EcoT38I GRGCYC 3 cut(s) 330, 390, 604
ErhI CCWWGG 2 cut(s) 517, 589
FaeI CATG 4 cut(s) 110, 191, 255, 593
FaqI GGGAC 4 cut(s) 94, 152, 227, 392
FatI CATG 4 cut(s) 106, 187, 251, 589
FauI CCCGC 4 cut(s) 493, 680, 839, 851
FbaI TGATCA 1 cut(s) 70
FblI GTMKAC 2 cut(s) 174, 435
Fnu4HI GCNGC 1 cut(s) 466
FokI GGATG 8 cut(s) 251, 331, 462, 560, 575, 798, 800, 840
FriOI GRGCYC 3 cut(s) 330, 390, 604
Fsp4HI GCNGC 1 cut(s) 466
FspBI CTAG 6 cut(s) 96, 119, 269, 509, 702, 797
GluI GCNGC 1 cut(s) 466
GsuI CTGGAG 2 cut(s) 111, 570
HapII CCGG 4 cut(s) 170, 384, 397, 462
HgaI GACGC 1 cut(s) 50
Hin1I GRCGYC 1 cut(s) 198
Hin1II CATG 4 cut(s) 110, 191, 255, 593
HinfI GANTC 1 cut(s) 361
HpaII CCGG 4 cut(s) 170, 384, 397, 462
HphI GGTGA 5 cut(s) 386, 406, 605, 885, 926
Hpy166II GTNNAC 5 cut(s) 175, 360, 436, 571, 697
Hpy188I TCNGA 1 cut(s) 421
Hpy8I GTNNAC 5 cut(s) 175, 360, 436, 571, 697
Hpy99I CGWCG 1 cut(s) 200
HpyAV CCTTC 3 cut(s) 124, 140, 221
HpyCH4III ACNGT 2 cut(s) 52, 655
HpyCH4IV ACGT 4 cut(s) 163, 198, 212, 432
HpyCH4V TGCA 3 cut(s) 4, 30, 752
HpyF3I CTNAG 2 cut(s) 409, 802
HpySE526I ACGT 4 cut(s) 163, 198, 212, 432
Hsp92I GRCGYC 1 cut(s) 198
Hsp92II CATG 4 cut(s) 110, 191, 255, 593
KroI GCCGGC 1 cut(s) 461
KroNI GCCGGC 1 cut(s) 463
Ksp22I TGATCA 1 cut(s) 70
KspI CCGCGG 1 cut(s) 847
Kzo9I GATC 4 cut(s) 70, 474, 580, 904
LmnI GCTCC 3 cut(s) 92, 189, 205
LweI GCATC 1 cut(s) 13
MaeI CTAG 6 cut(s) 96, 119, 269, 509, 702, 797
MaeII ACGT 4 cut(s) 163, 198, 212, 432
MaeIII GTNAC 4 cut(s) 262, 401, 412, 704
MalI GATC 4 cut(s) 72, 476, 582, 906
MbiI CCGCTC 1 cut(s) 468
MboI GATC 4 cut(s) 70, 474, 580, 904
MboII GAAGA 4 cut(s) 16, 433, 790, 919
MhlI GDGCHC 3 cut(s) 330, 390, 604
MluCI AATT 6 cut(s) 39, 152, 285, 366, 731, 889
MlyI GAGTC 1 cut(s) 355
MmeI TCCRAC 2 cut(s) 259, 706
MnlI CCTC 8 cut(s) 296, 402, 427, 505, 547, 736, 831, 859
Mph1103I ATGCAT 2 cut(s) 6, 754
MroNI GCCGGC 1 cut(s) 461
MseI TTAA 1 cut(s) 937
MslI CAYNNNNRTG 4 cut(s) 250, 355, 720, 772
MspA1I CMGCKG 1 cut(s) 846
MspI CCGG 4 cut(s) 170, 384, 397, 462
MspR9I CCNGG 5 cut(s) 90, 170, 235, 385, 816
MvaI CCWGG 3 cut(s) 90, 235, 816
MvnI CGCG 2 cut(s) 825, 846
NaeI GCCGGC 1 cut(s) 463
NciI CCSGG 2 cut(s) 170, 385
NcoI CCATGG 1 cut(s) 589
NdeII GATC 4 cut(s) 70, 474, 580, 904
NgoMIV GCCGGC 1 cut(s) 461
NlaIII CATG 4 cut(s) 110, 191, 255, 593
NlaIV GGNNCC 5 cut(s) 232, 388, 389, 602, 699
NmeAIII GCCGAG 1 cut(s) 766
NmuCI GTSAC 3 cut(s) 262, 412, 704
NsiI ATGCAT 2 cut(s) 6, 754
NspI RCATGY 1 cut(s) 110
NspV TTCGAA 1 cut(s) 144
OliI CACNNNNGTG 2 cut(s) 355, 720
PceI AGGCCT 1 cut(s) 134
PdiI GCCGGC 1 cut(s) 463
PflMI CCANNNNNTGG 2 cut(s) 257, 590
PfoI TCCNGGA 1 cut(s) 88
PkrI GCNGC 1 cut(s) 467
PleI GAGTC 1 cut(s) 355
PmaCI CACGTG 1 cut(s) 164
PmlI CACGTG 1 cut(s) 164
PpsI GAGTC 1 cut(s) 355
Ppu21I YACGTR 2 cut(s) 164, 433
Psp124BI GAGCTC 1 cut(s) 330
Psp6I CCWGG 3 cut(s) 88, 233, 814
PspCI CACGTG 1 cut(s) 164
PspGI CCWGG 3 cut(s) 88, 233, 814
PspN4I GGNNCC 5 cut(s) 232, 388, 389, 602, 699
PspOMI GGGCCC 2 cut(s) 386, 600
RsaI GTAC 1 cut(s) 570
RsaNI GTAC 1 cut(s) 569
RseI CAYNNNNRTG 4 cut(s) 250, 355, 720, 772
SacI GAGCTC 1 cut(s) 330
SacII CCGCGG 1 cut(s) 847
SaqAI TTAA 1 cut(s) 937
SatI GCNGC 1 cut(s) 466
Sau3AI GATC 4 cut(s) 70, 474, 580, 904
SchI GAGTC 1 cut(s) 355
ScrFI CCNGG 5 cut(s) 90, 170, 235, 385, 816
SduI GDGCHC 3 cut(s) 330, 390, 604
SfaNI GCATC 1 cut(s) 13
Sfr303I CCGCGG 1 cut(s) 847
SfuI TTCGAA 1 cut(s) 144
SgrBI CCGCGG 1 cut(s) 847
SinI GGWCC 3 cut(s) 86, 514, 697
SmiMI CAYNNNNRTG 4 cut(s) 250, 355, 720, 772
SpeI ACTAGT 1 cut(s) 268
Sse9I AATT 6 cut(s) 39, 152, 285, 366, 731, 889
SseBI AGGCCT 1 cut(s) 134
SsiI CCGC 8 cut(s) 248, 447, 466, 500, 687, 825, 844, 846
SspMI CTAG 6 cut(s) 96, 119, 269, 509, 702, 797
SstI GAGCTC 1 cut(s) 330
StuI AGGCCT 1 cut(s) 134
StyD4I CCNGG 5 cut(s) 88, 168, 233, 383, 814
StyI CCWWGG 2 cut(s) 517, 589
TaaI ACNGT 2 cut(s) 52, 655
TaiI ACGT 4 cut(s) 166, 201, 215, 435
TaqI TCGA 4 cut(s) 144, 195, 637, 907
TasI AATT 6 cut(s) 39, 152, 285, 366, 731, 889
TatI WGTACW 1 cut(s) 568
TauI GCSGC 1 cut(s) 468
Tru1I TTAA 1 cut(s) 937
Tru9I TTAA 1 cut(s) 937
TseFI GTSAC 3 cut(s) 262, 412, 704
Tsp45I GTSAC 3 cut(s) 262, 412, 704
TspDTI ATGAA 2 cut(s) 330, 911
Van91I CCANNNNNTGG 2 cut(s) 257, 590
VpaK11BI GGWCC 3 cut(s) 86, 514, 697
XagI CCTNNNNNAGG 1 cut(s) 509
XapI RAATTY 3 cut(s) 152, 731, 889
XceI RCATGY 1 cut(s) 110
XcmI CCANNNNNNNNNTGG 1 cut(s) 722
XmiI GTMKAC 2 cut(s) 174, 435
XspI CTAG 6 cut(s) 96, 119, 269, 509, 702, 797
ZraI GACGTC 1 cut(s) 199
Zsp2I ATGCAT 2 cut(s) 6, 754
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.