AT3G09350

Nucleotide exchange factor Fes1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0005504
Physical Location & Seq
Forward (+)
2870970 .. 2873332
2363 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G09350.2

Sequence Viewer

Length: 1092 bp
ATGGCGAAAGACGGACCTAATTGGGATGGTTTGCTTAAGTGGAGTCTCTCTCACGCCGACGGTACTCGACCCACTCGCCAGTTGAGTGAGGAGGATCGAAAATGGTTTATGGAGGCTATGCAATCGCAGACTGTAGATGTAGTCAAACGCATGAAGGAGATTACACTAGTTATGCAAACACCTGAACAAGTTTTGGTGGAACATGGAGTAACACCTGAAGATATCCAAGATTTGCTGGATGAGCTGCAAGAGCACGTTGAATCGATCGATATGGCTAACGATCTTCATTCAATTGGAGGCTTGGTTCCTCTTCTTAGTTTCCTTAAAAACTCTCATGCTAATATTCGGGCAAAAGCTGCGGATGTCGTTAGCACAATAGTTCAGAACAATCCTAGAAGTCAGGAACTTGTTATGGAAACCAATGCTCTGGAGTCATTGCTCTCGAATTTCACTTCAGACACTGATATTCACGCTCGAACCCAGGCTCTTGGCGCAATATCATCTTTGATTCGCCATAACAAACCAGGAGTAACGGCTTTCAAGCTTGCCAATGGTTATGCTGGCCTAAGAGACGCCTTGGCATCTGATAGTGTGAGATTTCAAAGGAAAGCCTTAAACTTGTTGCAATATCTACTGCAAGAGGATGATTCAGACCGCAGTATTGCCACTGGACTTGGATTCCCCCGAGTGATGATGCATCTTGCCTCTAGTGACGATGCTGAGATCAGAGAAGCCGCTCTTCGTGGATTACTTGAGCTTTCAAGAGAGAAAAACGATGGAAGTAGCAGCATAGATAAAAGCGATGAGAAACTGAGGCAGCTACTAGAAGAACGGATCAAAGGAATCACCTTGATGTCACAAGAGGATCTTGAAACGGTTAAAGAAGAGAGACAGCTCGTGGATTTACTGTGGAGCATTTGCTACAATGAACCTTCTTCTCTGAGAGAAAAAGGACTTGTCGTTCTTCCCGGTGAAGACGCATTGCCTCCTGATGTCGCTAGCAAGCTCTTTGAGCCTCCTCTTAGAGCTTCTGCAGCAAACCGTAATGCTACTGAGAAGAAAGATGAACCTATGAAACTACTTGGACCATAG

Protein Analysis

363

Amino Acids

40.4

Weight (kDa)

5.0

Isoelectric Point (pI)

42.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fes1 PF08609 4 - 59 1.4e-07 Nucleotide exchange factor Fes1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 737
AciI CCGC 3 cut(s) 359, 655, 735
AclWI GGATC 3 cut(s) 102, 842, 873
AcsI RAATTY 1 cut(s) 445
AcuI CTGAAG 2 cut(s) 237, 438
AcyI GRCGYC 1 cut(s) 573
AfaI GTAC 1 cut(s) 64
AflII CTTAAG 1 cut(s) 35
AgsI TTSAA 6 cut(s) 260, 291, 541, 602, 762, 872
AhlI ACTAGT 1 cut(s) 166
AjnI CCWGG 2 cut(s) 480, 523
AloI GAACNNNNNNTCC 4 cut(s) 288, 320, 945, 977
AluBI AGCT 8 cut(s) 244, 356, 544, 757, 820, 895, 1006, 1028
AluI AGCT 8 cut(s) 244, 356, 544, 757, 820, 895, 1006, 1028
Alw21I GWGCWC 1 cut(s) 255
Alw26I GTCTC 3 cut(s) 50, 564, 883
AlwI GGATC 3 cut(s) 102, 842, 873
AlwNI CAGNNNCTG 1 cut(s) 461
Ama87I CYCGRG 1 cut(s) 684
AoxI GGCC 1 cut(s) 562
ApeKI GCWGC 5 cut(s) 244, 356, 786, 817, 1034
ApoI RAATTY 1 cut(s) 445
AspLEI GCGC 1 cut(s) 494
AspS9I GGNCC 2 cut(s) 14, 1085
AsuC2I CCSGG 1 cut(s) 969
AsuHPI GGTGA 2 cut(s) 838, 983
AsuNHI GCTAGC 1 cut(s) 998
AvaI CYCGRG 1 cut(s) 684
AvaII GGWCC 2 cut(s) 14, 1085
BauI CACGAG 1 cut(s) 896
BbsI GAAGAC 1 cut(s) 981
Bbv12I GWGCWC 1 cut(s) 255
BbvI GCAGC 5 cut(s) 231, 343, 798, 829, 1046
BccI CCATC 2 cut(s) 20, 770
BceAI ACGGC 1 cut(s) 549
BciT130I CCWGG 2 cut(s) 482, 525
BcnI CCSGG 1 cut(s) 969
BcoDI GTCTC 3 cut(s) 50, 564, 883
BcuI ACTAGT 1 cut(s) 166
BfaI CTAG 5 cut(s) 167, 393, 708, 824, 999
BfmI CTRYAG 2 cut(s) 132, 1032
BfrI CTTAAG 1 cut(s) 35
BisI GCNGC 6 cut(s) 245, 357, 735, 787, 818, 1035
BlsI GCNGC 6 cut(s) 246, 358, 736, 788, 819, 1036
Bme1390I CCNGG 3 cut(s) 482, 525, 969
Bme18I GGWCC 2 cut(s) 14, 1085
BmeT110I CYCGRG 1 cut(s) 684
BmgT120I GGNCC 2 cut(s) 14, 1085
BmiI GGNNCC 1 cut(s) 306
BmrFI CCNGG 3 cut(s) 482, 525, 969
BmsI GCATC 4 cut(s) 590, 684, 706, 706
BmtI GCTAGC 1 cut(s) 1002
BpiI GAAGAC 1 cut(s) 981
BplI GAGNNNNNCTC 2 cut(s) 34, 66
BpmI CTGGAG 1 cut(s) 449
BpuEI CTTGAG 1 cut(s) 773
BpuMI CCSGG 1 cut(s) 969
Bsa29I ATCGAT 2 cut(s) 263, 267
BsaHI GRCGYC 1 cut(s) 573
BsaJI CCNNGG 2 cut(s) 480, 576
BsaXI ACNNNNNCTCC 2 cut(s) 288, 318
Bse1I ACTGG 2 cut(s) 79, 673
Bse3DI GCAATG 2 cut(s) 434, 980
BseBI CCWGG 2 cut(s) 482, 525
BseCI ATCGAT 2 cut(s) 263, 267
BseDI CCNNGG 2 cut(s) 480, 576
BseGI GGATG 4 cut(s) 31, 244, 367, 649
BseMI GCAATG 2 cut(s) 434, 980
BseMII CTCAG 4 cut(s) 711, 803, 932, 1044
BseNI ACTGG 2 cut(s) 79, 673
BseRI GAGGAG 2 cut(s) 104, 1008
BseXI GCAGC 5 cut(s) 231, 343, 798, 829, 1046
Bsh1285I CGRYCG 1 cut(s) 267
BshFI GGCC 1 cut(s) 564
BshVI ATCGAT 2 cut(s) 263, 267
BsiEI CGRYCG 1 cut(s) 267
BsiHKAI GWGCWC 1 cut(s) 255
BsiHKCI CYCGRG 1 cut(s) 684
BsiSI CCGG 1 cut(s) 969
BsmAI GTCTC 3 cut(s) 50, 564, 883
BsmBI CGTCTC 1 cut(s) 564
BsnI GGCC 1 cut(s) 564
BsoBI CYCGRG 1 cut(s) 684
Bsp1286I GDGCHC 1 cut(s) 255
Bsp143I GATC 6 cut(s) 94, 264, 280, 723, 834, 865
BspACI CCGC 3 cut(s) 359, 655, 735
BspANI GGCC 1 cut(s) 564
BspCNI CTCAG 4 cut(s) 712, 804, 933, 1045
BspDI ATCGAT 2 cut(s) 263, 267
BspLI GGNNCC 1 cut(s) 306
BspMAI CTGCAG 1 cut(s) 1036
BspOI GCTAGC 1 cut(s) 1002
BspPI GGATC 3 cut(s) 102, 842, 873
BspQI GCTCTTC 1 cut(s) 744
BspTI CTTAAG 1 cut(s) 35
BsrBI CCGCTC 1 cut(s) 737
BsrDI GCAATG 2 cut(s) 434, 980
BsrI ACTGG 2 cut(s) 79, 673
BssECI CCNNGG 2 cut(s) 480, 576
BssMI GATC 6 cut(s) 94, 264, 280, 723, 834, 865
BssNI GRCGYC 1 cut(s) 573
BssSI CACGAG 1 cut(s) 896
BssT1I CCWWGG 1 cut(s) 576
Bst2BI CACGAG 1 cut(s) 896
Bst2UI CCWGG 2 cut(s) 482, 525
Bst4CI ACNGT 5 cut(s) 62, 133, 877, 909, 1043
Bst6I CTCTTC 3 cut(s) 315, 744, 879
BstACI GRCGYC 1 cut(s) 573
BstAFI CTTAAG 1 cut(s) 35
BstAPI GCANNNNNTGC 1 cut(s) 356
BstC8I GCNNGC 4 cut(s) 546, 562, 1000, 1004
BstDEI CTNAG 7 cut(s) 314, 566, 720, 812, 941, 1022, 1053
BstF5I GGATG 4 cut(s) 31, 244, 367, 649
BstHHI GCGC 1 cut(s) 494
BstKTI GATC 6 cut(s) 97, 267, 283, 726, 837, 868
BstMAI GTCTC 3 cut(s) 50, 564, 883
BstMBI GATC 6 cut(s) 94, 264, 280, 723, 834, 865
BstMCI CGRYCG 1 cut(s) 267
BstMWI GCNNNNNNNGC 6 cut(s) 241, 250, 356, 491, 1012, 1034
BstNI CCWGG 2 cut(s) 482, 525
BstSCI CCNGG 3 cut(s) 480, 523, 967
BstSFI CTRYAG 2 cut(s) 132, 1032
BstV1I GCAGC 5 cut(s) 231, 343, 798, 829, 1046
BstV2I GAAGAC 1 cut(s) 981
BstX2I RGATCY 1 cut(s) 865
BstXI CCANNNNNNTGG 2 cut(s) 427, 488
BstYI RGATCY 1 cut(s) 865
Bsu15I ATCGAT 2 cut(s) 263, 267
BsuRI GGCC 1 cut(s) 564
BsuTUI ATCGAT 2 cut(s) 263, 267
BtgZI GCGATG 1 cut(s) 816
BtsCI GGATG 4 cut(s) 31, 244, 367, 649
BtsIMutI CAGTG 2 cut(s) 459, 666
Cac8I GCNNGC 4 cut(s) 546, 562, 1000, 1004
CaiI CAGNNNCTG 1 cut(s) 461
CfoI GCGC 1 cut(s) 494
Cfr13I GGNCC 2 cut(s) 14, 1085
ClaI ATCGAT 2 cut(s) 263, 267
CseI GACGC 2 cut(s) 581, 986
Csp6I GTAC 1 cut(s) 63
CspCI CAANNNNNGTGG 2 cut(s) 655, 690
CviAII CATG 3 cut(s) 151, 203, 335
CviQI GTAC 1 cut(s) 63
DdeI CTNAG 7 cut(s) 314, 566, 720, 812, 941, 1022, 1053
DpnI GATC 6 cut(s) 96, 266, 282, 725, 836, 867
DpnII GATC 6 cut(s) 94, 264, 280, 723, 834, 865
Eam1104I CTCTTC 3 cut(s) 315, 744, 879
EarI CTCTTC 3 cut(s) 315, 744, 879
Eco130I CCWWGG 1 cut(s) 576
Eco32I GATATC 1 cut(s) 223
Eco47I GGWCC 2 cut(s) 14, 1085
Eco57I CTGAAG 2 cut(s) 237, 438
Eco88I CYCGRG 1 cut(s) 684
EcoRII CCWGG 2 cut(s) 480, 523
EcoRV GATATC 1 cut(s) 223
EcoT14I CCWWGG 1 cut(s) 576
EcoT22I ATGCAT 1 cut(s) 699
ErhI CCWWGG 1 cut(s) 576
Esp3I CGTCTC 1 cut(s) 564
FaeI CATG 3 cut(s) 154, 206, 338
FalI AAGNNNNNCTT 4 cut(s) 723, 755, 852, 884
FatI CATG 3 cut(s) 150, 202, 334
Fnu4HI GCNGC 6 cut(s) 245, 357, 735, 787, 818, 1035
FokI GGATG 4 cut(s) 38, 251, 374, 656
Fsp4HI GCNGC 6 cut(s) 245, 357, 735, 787, 818, 1035
FspBI CTAG 5 cut(s) 167, 393, 708, 824, 999
GlaI GCGC 1 cut(s) 493
GluI GCNGC 6 cut(s) 245, 357, 735, 787, 818, 1035
GsuI CTGGAG 1 cut(s) 449
HaeIII GGCC 1 cut(s) 564
HapII CCGG 1 cut(s) 969
HgaI GACGC 2 cut(s) 581, 986
HhaI GCGC 1 cut(s) 494
Hin1I GRCGYC 1 cut(s) 573
Hin1II CATG 3 cut(s) 154, 206, 338
Hin6I GCGC 1 cut(s) 492
HinP1I GCGC 1 cut(s) 492
HindIII AAGCTT 1 cut(s) 542
HinfI GANTC 7 cut(s) 43, 260, 431, 508, 647, 678, 843
HpaII CCGG 1 cut(s) 969
HphI GGTGA 2 cut(s) 838, 983
Hpy188I TCNGA 6 cut(s) 384, 457, 586, 652, 728, 942
Hpy188III TCNNGA 6 cut(s) 401, 428, 442, 762, 869, 989
Hpy99I CGWCG 1 cut(s) 62
HpyAV CCTTC 2 cut(s) 148, 942
HpyCH4III ACNGT 5 cut(s) 62, 133, 877, 909, 1043
HpyCH4IV ACGT 1 cut(s) 255
HpyCH4V TGCA 7 cut(s) 121, 175, 247, 625, 637, 697, 1034
HpyF10VI GCNNNNNNNGC 6 cut(s) 241, 250, 356, 491, 1012, 1034
HpyF3I CTNAG 7 cut(s) 314, 566, 720, 812, 941, 1022, 1053
HpySE526I ACGT 1 cut(s) 255
Hsp92I GRCGYC 1 cut(s) 573
Hsp92II CATG 3 cut(s) 154, 206, 338
HspAI GCGC 1 cut(s) 492
Kzo9I GATC 6 cut(s) 94, 264, 280, 723, 834, 865
LguI GCTCTTC 1 cut(s) 744
LmnI GCTCC 1 cut(s) 912
Lsp1109I GCAGC 5 cut(s) 231, 343, 798, 829, 1046
LweI GCATC 4 cut(s) 590, 684, 706, 706
MaeI CTAG 5 cut(s) 167, 393, 708, 824, 999
MaeII ACGT 1 cut(s) 255
MaeIII GTNAC 4 cut(s) 208, 529, 710, 855
MalI GATC 6 cut(s) 96, 266, 282, 725, 836, 867
MbiI CCGCTC 1 cut(s) 737
MboI GATC 6 cut(s) 94, 264, 280, 723, 834, 865
MfeI CAATTG 1 cut(s) 291
MflI RGATCY 1 cut(s) 865
MhlI GDGCHC 1 cut(s) 255
MluCI AATT 3 cut(s) 19, 291, 445
MlyI GAGTC 2 cut(s) 52, 440
Mph1103I ATGCAT 1 cut(s) 699
MseI TTAA 4 cut(s) 36, 324, 614, 879
MslI CAYNNNNRTG 1 cut(s) 851
MspCI CTTAAG 1 cut(s) 35
MspI CCGG 1 cut(s) 969
MspR9I CCNGG 3 cut(s) 482, 525, 969
MunI CAATTG 1 cut(s) 291
MvaI CCWGG 2 cut(s) 482, 525
MwoI GCNNNNNNNGC 6 cut(s) 241, 250, 356, 491, 1012, 1034
NciI CCSGG 1 cut(s) 969
NdeII GATC 6 cut(s) 94, 264, 280, 723, 834, 865
NheI GCTAGC 1 cut(s) 998
NlaIII CATG 3 cut(s) 154, 206, 338
NlaIV GGNNCC 1 cut(s) 306
NmuCI GTSAC 2 cut(s) 710, 855
NsiI ATGCAT 1 cut(s) 699
PciSI GCTCTTC 1 cut(s) 744
PfeI GAWTC 5 cut(s) 260, 508, 647, 678, 843
PkrI GCNGC 6 cut(s) 246, 358, 736, 788, 819, 1036
Ple19I CGATCG 1 cut(s) 267
PleI GAGTC 2 cut(s) 51, 439
PpsI GAGTC 2 cut(s) 51, 439
Psp6I CCWGG 2 cut(s) 480, 523
PspGI CCWGG 2 cut(s) 480, 523
PspN4I GGNNCC 1 cut(s) 306
PspPI GGNCC 2 cut(s) 14, 1085
PstI CTGCAG 1 cut(s) 1036
PstNI CAGNNNCTG 1 cut(s) 461
PsuI RGATCY 1 cut(s) 865
PvuI CGATCG 1 cut(s) 267
RsaI GTAC 1 cut(s) 64
RsaNI GTAC 1 cut(s) 63
RseI CAYNNNNRTG 1 cut(s) 851
SapI GCTCTTC 1 cut(s) 744
SaqAI TTAA 4 cut(s) 36, 324, 614, 879
SatI GCNGC 6 cut(s) 245, 357, 735, 787, 818, 1035
Sau3AI GATC 6 cut(s) 94, 264, 280, 723, 834, 865
Sau96I GGNCC 2 cut(s) 14, 1085
SchI GAGTC 2 cut(s) 52, 440
ScrFI CCNGG 3 cut(s) 482, 525, 969
SduI GDGCHC 1 cut(s) 255
SfaNI GCATC 4 cut(s) 590, 684, 706, 706
SfcI CTRYAG 2 cut(s) 132, 1032
SinI GGWCC 2 cut(s) 14, 1085
SmiMI CAYNNNNRTG 1 cut(s) 851
SmlI CTYRAG 2 cut(s) 35, 752
SmoI CTYRAG 2 cut(s) 35, 752
SpeI ACTAGT 1 cut(s) 166
Sse9I AATT 3 cut(s) 19, 291, 445
SsiI CCGC 3 cut(s) 359, 655, 735
SspI AATATT 1 cut(s) 343
SspMI CTAG 5 cut(s) 167, 393, 708, 824, 999
StyD4I CCNGG 3 cut(s) 480, 523, 967
StyI CCWWGG 1 cut(s) 576
TaaI ACNGT 5 cut(s) 62, 133, 877, 909, 1043
TaiI ACGT 1 cut(s) 258
TaqI TCGA 6 cut(s) 67, 97, 263, 267, 443, 475
TasI AATT 3 cut(s) 19, 291, 445
TauI GCSGC 1 cut(s) 737
TfiI GAWTC 5 cut(s) 260, 508, 647, 678, 843
Tru1I TTAA 4 cut(s) 36, 324, 614, 879
Tru9I TTAA 4 cut(s) 36, 324, 614, 879
TscAI CASTG 2 cut(s) 466, 673
TseFI GTSAC 2 cut(s) 710, 855
TseI GCWGC 5 cut(s) 244, 356, 786, 817, 1034
Tsp45I GTSAC 2 cut(s) 710, 855
TspDTI ATGAA 5 cut(s) 167, 275, 942, 1080, 1088
TspGWI ACGGA 2 cut(s) 27, 847
TspRI CASTG 2 cut(s) 466, 673
Vha464I CTTAAG 1 cut(s) 35
VpaK11BI GGWCC 2 cut(s) 14, 1085
XapI RAATTY 1 cut(s) 445
XspI CTAG 5 cut(s) 167, 393, 708, 824, 999
Zsp2I ATGCAT 1 cut(s) 699
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.