AT3G10020

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0011126
Physical Location & Seq
Reverse (-)
3090806 .. 3091922
1117 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G10020.2

Sequence Viewer

Length: 450 bp
ATGCAGGCGGTATCAGTTGATGAAACCAAAAACACGGTGGTGCTACGGGCGGAGCACAAGAATGAAGAAGGCCGGAAATCTGTTGATAAGTTCGAAGTGAAGACACGTAATCCGGAGACGATCAAGAGCGTTGAGCGGAAGCTTATGGAGAAAGGTGTTCAACGGATGGATCGTCATCCTTCCGATGGGATTCCTCTCAGGCGACCACCATCGAAGTCAGGTCACGGCGGGAAGTACACGTGGGAAGGACCTGATCGGATGGAGGATTACGAGATGCAGCCGGATCCACCGGCGATGGATGAAGGAGATCCTAATTACGACGAGGAACAGGTGAAGAAGAGTGGTGATGACGTGGCGGTTGAGCTTGTGAAAGGAGAGGTTGAGGTGGCTAAGGAGGCTCCGGCTGGTGTTGCTAGGGTTGAGGTTGATCCTCGCTTGGTCGGTCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

149

Amino Acids

16.74

Weight (kDa)

5.24

Isoelectric Point (pI)

25.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 136
AccIII TCCGGA 1 cut(s) 112
AciI CCGC 5 cut(s) 8, 50, 136, 228, 356
AclWI GGATC 5 cut(s) 177, 278, 291, 302, 422
AcvI CACGTG 1 cut(s) 240
AfaI GTAC 1 cut(s) 236
AfiI CCNNNNNNNGG 1 cut(s) 185
AflIII ACRYGT 2 cut(s) 104, 237
AgsI TTSAA 1 cut(s) 161
AjiI CACGTC 1 cut(s) 352
AleI CACNNNNGTG 1 cut(s) 38
AluBI AGCT 2 cut(s) 142, 364
AluI AGCT 2 cut(s) 142, 364
Alw21I GWGCWC 1 cut(s) 57
Alw26I GTCTC 1 cut(s) 110
AlwI GGATC 5 cut(s) 177, 278, 291, 302, 422
Aor13HI TCCGGA 1 cut(s) 112
AoxI GGCC 1 cut(s) 70
ApeKI GCWGC 1 cut(s) 277
AspS9I GGNCC 1 cut(s) 248
AsuHPI GGTGA 2 cut(s) 343, 356
AsuII TTCGAA 1 cut(s) 93
AvaII GGWCC 1 cut(s) 248
BamHI GGATCC 1 cut(s) 283
BbrPI CACGTG 1 cut(s) 240
BbsI GAAGAC 1 cut(s) 107
Bbv12I GWGCWC 1 cut(s) 57
BbvI GCAGC 1 cut(s) 289
BccI CCATC 5 cut(s) 160, 179, 217, 253, 289
BceAI ACGGC 1 cut(s) 241
BcoDI GTCTC 1 cut(s) 110
BfaI CTAG 1 cut(s) 414
BisI GCNGC 1 cut(s) 278
BlsI GCNGC 1 cut(s) 279
Bme18I GGWCC 1 cut(s) 248
BmgBI CACGTC 1 cut(s) 352
BmgT120I GGNCC 1 cut(s) 248
BmiI GGNNCC 2 cut(s) 285, 399
BmsI GCATC 1 cut(s) 264
BpiI GAAGAC 1 cut(s) 107
Bpu10I CCTNAGC 1 cut(s) 390
Bpu14I TTCGAA 1 cut(s) 93
BsaAI YACGTR 2 cut(s) 107, 240
BsaBI GATNNNNATC 1 cut(s) 174
BsaWI WCCGGW 1 cut(s) 112
Bsc4I CCNNNNNNNGG 1 cut(s) 185
Bse118I RCCGGY 1 cut(s) 289
Bse8I GATNNNNATC 1 cut(s) 174
BseAI TCCGGA 1 cut(s) 112
BseGI GGATG 4 cut(s) 171, 175, 264, 304
BseJI GATNNNNATC 1 cut(s) 174
BseLI CCNNNNNNNGG 1 cut(s) 185
BseMII CTCAG 1 cut(s) 211
BseXI GCAGC 1 cut(s) 289
Bsh1285I CGRYCG 1 cut(s) 445
BshFI GGCC 1 cut(s) 72
BsiEI CGRYCG 1 cut(s) 445
BsiHKAI GWGCWC 1 cut(s) 57
BsiSI CCGG 5 cut(s) 73, 113, 281, 290, 401
BslI CCNNNNNNNGG 1 cut(s) 185
BsmAI GTCTC 1 cut(s) 110
BsmBI CGTCTC 1 cut(s) 110
BsnI GGCC 1 cut(s) 72
Bsp119I TTCGAA 1 cut(s) 93
Bsp1286I GDGCHC 1 cut(s) 57
Bsp13I TCCGGA 1 cut(s) 112
Bsp143I GATC 6 cut(s) 120, 169, 253, 283, 307, 427
BspACI CCGC 5 cut(s) 8, 50, 136, 228, 356
BspANI GGCC 1 cut(s) 72
BspCNI CTCAG 1 cut(s) 210
BspEI TCCGGA 1 cut(s) 112
BspLI GGNNCC 2 cut(s) 285, 399
BspPI GGATC 5 cut(s) 177, 278, 291, 302, 422
BspT104I TTCGAA 1 cut(s) 93
BsrBI CCGCTC 1 cut(s) 136
BsrFI RCCGGY 1 cut(s) 289
BssAI RCCGGY 1 cut(s) 289
BssMI GATC 6 cut(s) 120, 169, 253, 283, 307, 427
Bst4CI ACNGT 1 cut(s) 37
Bst6I CTCTTC 1 cut(s) 332
BstBAI YACGTR 2 cut(s) 107, 240
BstBI TTCGAA 1 cut(s) 93
BstC8I GCNNGC 1 cut(s) 6
BstDEI CTNAG 2 cut(s) 197, 390
BstF5I GGATG 4 cut(s) 171, 175, 264, 304
BstKTI GATC 6 cut(s) 123, 172, 256, 286, 310, 430
BstMAI GTCTC 1 cut(s) 110
BstMBI GATC 6 cut(s) 120, 169, 253, 283, 307, 427
BstMCI CGRYCG 1 cut(s) 445
BstMWI GCNNNNNNNGC 2 cut(s) 395, 410
BstV1I GCAGC 1 cut(s) 289
BstV2I GAAGAC 1 cut(s) 107
BstX2I RGATCY 2 cut(s) 283, 307
BstYI RGATCY 2 cut(s) 283, 307
BsuRI GGCC 1 cut(s) 72
BtgZI GCGATG 1 cut(s) 308
BtrI CACGTC 1 cut(s) 352
BtsCI GGATG 4 cut(s) 171, 175, 264, 304
Cac8I GCNNGC 1 cut(s) 6
Cfr10I RCCGGY 1 cut(s) 289
Cfr13I GGNCC 1 cut(s) 248
Csp6I GTAC 1 cut(s) 235
CviJI RGCY 7 cut(s) 72, 142, 280, 364, 389, 398, 404
CviKI_1 RGCY 7 cut(s) 72, 142, 280, 364, 389, 398, 404
CviQI GTAC 1 cut(s) 235
DdeI CTNAG 2 cut(s) 197, 390
DpnI GATC 6 cut(s) 122, 171, 255, 285, 309, 429
DpnII GATC 6 cut(s) 120, 169, 253, 283, 307, 427
Eam1104I CTCTTC 1 cut(s) 332
EarI CTCTTC 1 cut(s) 332
EciI GGCGGA 1 cut(s) 65
Eco47I GGWCC 1 cut(s) 248
Eco72I CACGTG 1 cut(s) 240
EcoO109I RGGNCCY 1 cut(s) 248
Esp3I CGTCTC 1 cut(s) 110
FaiI YATR 1 cut(s) 146
FauI CCCGC 1 cut(s) 221
Fnu4HI GCNGC 1 cut(s) 278
FokI GGATG 4 cut(s) 162, 178, 271, 311
Fsp4HI GCNGC 1 cut(s) 278
FspBI CTAG 1 cut(s) 414
GluI GCNGC 1 cut(s) 278
HaeIII GGCC 1 cut(s) 72
HapII CCGG 5 cut(s) 73, 113, 281, 290, 401
HindIII AAGCTT 1 cut(s) 140
HinfI GANTC 1 cut(s) 190
HpaII CCGG 5 cut(s) 73, 113, 281, 290, 401
HphI GGTGA 2 cut(s) 343, 356
Hpy166II GTNNAC 1 cut(s) 237
Hpy188I TCNGA 2 cut(s) 184, 258
Hpy188III TCNNGA 2 cut(s) 113, 124
Hpy8I GTNNAC 1 cut(s) 237
Hpy99I CGWCG 1 cut(s) 323
HpyAV CCTTC 4 cut(s) 62, 189, 239, 296
HpyCH4III ACNGT 1 cut(s) 37
HpyCH4IV ACGT 3 cut(s) 106, 239, 351
HpyCH4V TGCA 2 cut(s) 4, 277
HpyF10VI GCNNNNNNNGC 2 cut(s) 395, 410
HpyF3I CTNAG 2 cut(s) 197, 390
HpySE526I ACGT 3 cut(s) 106, 239, 351
Kpn2I TCCGGA 1 cut(s) 112
Kzo9I GATC 6 cut(s) 120, 169, 253, 283, 307, 427
LmnI GCTCC 2 cut(s) 52, 403
Lsp1109I GCAGC 1 cut(s) 289
LweI GCATC 1 cut(s) 264
MaeI CTAG 1 cut(s) 414
MaeII ACGT 3 cut(s) 106, 239, 351
MaeIII GTNAC 1 cut(s) 221
MalI GATC 6 cut(s) 122, 171, 255, 285, 309, 429
MbiI CCGCTC 1 cut(s) 136
MboI GATC 6 cut(s) 120, 169, 253, 283, 307, 427
MboII GAAGA 4 cut(s) 77, 112, 346, 349
MflI RGATCY 2 cut(s) 283, 307
MhlI GDGCHC 1 cut(s) 57
MluCI AATT 1 cut(s) 313
MnlI CCTC 8 cut(s) 204, 256, 316, 370, 376, 388, 415, 441
MroI TCCGGA 1 cut(s) 112
MslI CAYNNNNRTG 2 cut(s) 38, 60
MspI CCGG 5 cut(s) 73, 113, 281, 290, 401
MwoI GCNNNNNNNGC 2 cut(s) 395, 410
NdeII GATC 6 cut(s) 120, 169, 253, 283, 307, 427
NlaIV GGNNCC 2 cut(s) 285, 399
NmuCI GTSAC 1 cut(s) 221
NspV TTCGAA 1 cut(s) 93
OliI CACNNNNGTG 1 cut(s) 38
PcsI WCGNNNNNNNCGW 1 cut(s) 169
PfeI GAWTC 1 cut(s) 190
PkrI GCNGC 1 cut(s) 279
PmaCI CACGTG 1 cut(s) 240
PmlI CACGTG 1 cut(s) 240
Ppu21I YACGTR 2 cut(s) 107, 240
PpuMI RGGWCCY 1 cut(s) 248
Psp5II RGGWCCY 1 cut(s) 248
PspCI CACGTG 1 cut(s) 240
PspN4I GGNNCC 2 cut(s) 285, 399
PspPI GGNCC 1 cut(s) 248
PspPPI RGGWCCY 1 cut(s) 248
PsuI RGATCY 2 cut(s) 283, 307
RsaI GTAC 1 cut(s) 236
RsaNI GTAC 1 cut(s) 235
RseI CAYNNNNRTG 2 cut(s) 38, 60
SatI GCNGC 1 cut(s) 278
Sau3AI GATC 6 cut(s) 120, 169, 253, 283, 307, 427
Sau96I GGNCC 1 cut(s) 248
SduI GDGCHC 1 cut(s) 57
SfaNI GCATC 1 cut(s) 264
SfuI TTCGAA 1 cut(s) 93
SgrAI CRCCGGYG 1 cut(s) 289
SinI GGWCC 1 cut(s) 248
SmiMI CAYNNNNRTG 2 cut(s) 38, 60
Sse9I AATT 1 cut(s) 313
SsiI CCGC 5 cut(s) 8, 50, 136, 228, 356
SspMI CTAG 1 cut(s) 414
TaaI ACNGT 1 cut(s) 37
TaiI ACGT 3 cut(s) 109, 242, 354
TaqI TCGA 2 cut(s) 93, 212
TaqII GACCGA 1 cut(s) 431
TasI AATT 1 cut(s) 313
TatI WGTACW 1 cut(s) 234
TfiI GAWTC 1 cut(s) 190
TseFI GTSAC 1 cut(s) 221
TseI GCWGC 1 cut(s) 277
Tsp45I GTSAC 1 cut(s) 221
TspDTI ATGAA 3 cut(s) 36, 78, 315
TspGWI ACGGA 1 cut(s) 178
VpaK11BI GGWCC 1 cut(s) 248
XcmI CCANNNNNNNNNTGG 1 cut(s) 34
XspI CTAG 1 cut(s) 414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.