Rroxscaffold_6G00409420

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0011126
Physical Location & Seq
Reverse (-)
31923069 .. 31925640
2572 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00409420.1

Sequence Viewer

Length: 729 bp
ATGGCGTGGGACCCACCACGTGGGCTTGCTGATGCATGCAAGTGGGCTACACTTGCTTCTCACACTTACCAGAAGAATGTAACACAACGTGGGAATCTTCTACAGCACCACATGGCAAAAACTCGAAGCTTCACCTTTTCTAGCACCTTCCGGTGTTATCCGAACCCTGCTCTTCCATATATACACCCACTACGAGACGACTCCCATCTCTCACTGAAACAAAACAAGAAAACTCGCGAATCACAGTTAATCACGATGAAACCCATCGACGGCAAGAAAGACAAGGTTGTCATCCGAGCGGTGAGCCACAACGAGGAGGGCCGGAAGATAGTGGAGAAGACGGAGCTCAAAACCCGCAACATCGACACCATCAAGTACGTGGAGAAGAAGCTCGTAGACAAGGGATTGCAGGGCCGGGAACGCCACCCCATCGATGGATTGGGAATCGGACAACCGCCGCCGAAGTCGGGCCACGGCGGGAAGTACACGTGGGAGGGACCCGGCGACATGGCGGAGAACGAGCTGGACCCTGTCCCGGCGGCCATAGATGAGAGGGATCCCAACTATGTGGATGAGGAGGAGGAGGAGAGGATTGTGAAGGGGGAGGAGGCTGACGTGGCGGAAATGGTGGTGGGGGAGGTGGAGGTGGCGAAGGCGGTGGAGAACAGGGAGGGGGTGACTAGAGTTGAGGTTGATCCAAAGCTCGGTGCTCAGCCTTTGTTCACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

242

Amino Acids

27.21

Weight (kDa)

6.27

Isoelectric Point (pI)

31.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 287
AccB7I CCANNNNNTGG 1 cut(s) 20
AccBSI CCGCTC 1 cut(s) 299
AccI GTMKAC 1 cut(s) 396
AccII CGCG 1 cut(s) 237
AciI CCGC 9 cut(s) 299, 355, 455, 458, 477, 512, 539, 620, 656
AclWI GGATC 3 cut(s) 551, 564, 689
AcoI YGGCCR 1 cut(s) 540
AcvI CACGTG 2 cut(s) 20, 489
AdeI CACNNNGTG 2 cut(s) 20, 89
AfaI GTAC 2 cut(s) 377, 485
AfiI CCNNNNNNNGG 7 cut(s) 20, 269, 313, 434, 467, 535, 704
AflIII ACRYGT 1 cut(s) 486
AjiI CACGTC 1 cut(s) 616
AluBI AGCT 5 cut(s) 129, 346, 391, 523, 703
AluI AGCT 5 cut(s) 129, 346, 391, 523, 703
Alw21I GWGCWC 2 cut(s) 348, 712
Alw26I GTCTC 1 cut(s) 189
AlwI GGATC 3 cut(s) 551, 564, 689
AoxI GGCC 4 cut(s) 319, 412, 469, 540
AspS9I GGNCC 6 cut(s) 10, 319, 412, 469, 497, 526
AsuC2I CCSGG 3 cut(s) 416, 501, 536
AsuHPI GGTGA 3 cut(s) 124, 313, 688
AvaII GGWCC 3 cut(s) 10, 497, 526
BamHI GGATCC 1 cut(s) 556
BanII GRGCYC 1 cut(s) 348
BbrPI CACGTG 2 cut(s) 20, 489
BbsI GAAGAC 1 cut(s) 344
Bbv12I GWGCWC 2 cut(s) 348, 712
BccI CCATC 5 cut(s) 213, 272, 377, 428, 437
BceAI ACGGC 2 cut(s) 286, 490
BcnI CCSGG 3 cut(s) 416, 501, 536
BcoDI GTCTC 1 cut(s) 189
BfaI CTAG 2 cut(s) 141, 681
BfmI CTRYAG 1 cut(s) 101
BisI GCNGC 2 cut(s) 458, 540
BlpI GCTNAGC 1 cut(s) 711
BlsI GCNGC 2 cut(s) 459, 541
Bme1390I CCNGG 3 cut(s) 416, 501, 536
Bme18I GGWCC 3 cut(s) 10, 497, 526
BmgBI CACGTC 1 cut(s) 616
BmgT120I GGNCC 6 cut(s) 10, 319, 412, 469, 497, 526
BmiI GGNNCC 6 cut(s) 11, 12, 498, 499, 528, 558
BmrFI CCNGG 3 cut(s) 416, 501, 536
BmsI GCATC 1 cut(s) 22
BpiI GAAGAC 1 cut(s) 344
Bpu1102I GCTNAGC 1 cut(s) 711
BpuMI CCSGG 3 cut(s) 416, 501, 536
Bsa29I ATCGAT 1 cut(s) 432
BsaAI YACGTR 3 cut(s) 20, 379, 489
BsaJI CCNNGG 1 cut(s) 472
BsaWI WCCGGW 1 cut(s) 150
Bsc4I CCNNNNNNNGG 7 cut(s) 20, 269, 313, 434, 467, 535, 704
BseCI ATCGAT 1 cut(s) 432
BseDI CCNNGG 1 cut(s) 472
BseGI GGATG 2 cut(s) 291, 577
BseLI CCNNNNNNNGG 7 cut(s) 20, 269, 313, 434, 467, 535, 704
BseMII CTCAG 1 cut(s) 725
BseRI GAGGAG 6 cut(s) 329, 590, 593, 596, 599, 620
Bsh1236I CGCG 1 cut(s) 237
BshFI GGCC 4 cut(s) 321, 414, 471, 542
BshVI ATCGAT 1 cut(s) 432
BsiHKAI GWGCWC 2 cut(s) 348, 712
BsiSI CCGG 5 cut(s) 151, 322, 415, 501, 536
BslFI GGGAC 3 cut(s) 23, 510, 518
BslI CCNNNNNNNGG 7 cut(s) 20, 269, 313, 434, 467, 535, 704
BsmAI GTCTC 1 cut(s) 189
BsmBI CGTCTC 1 cut(s) 189
BsmFI GGGAC 3 cut(s) 23, 510, 518
BsnI GGCC 4 cut(s) 321, 414, 471, 542
Bsp1286I GDGCHC 2 cut(s) 348, 712
Bsp143I GATC 2 cut(s) 556, 694
Bsp1720I GCTNAGC 1 cut(s) 711
Bsp68I TCGCGA 1 cut(s) 237
BspACI CCGC 9 cut(s) 299, 355, 455, 458, 477, 512, 539, 620, 656
BspANI GGCC 4 cut(s) 321, 414, 471, 542
BspCNI CTCAG 1 cut(s) 724
BspDI ATCGAT 1 cut(s) 432
BspFNI CGCG 1 cut(s) 237
BspLI GGNNCC 6 cut(s) 11, 12, 498, 499, 528, 558
BspPI GGATC 3 cut(s) 551, 564, 689
BspQI GCTCTTC 1 cut(s) 177
BsrBI CCGCTC 1 cut(s) 299
BssECI CCNNGG 1 cut(s) 472
BssMI GATC 2 cut(s) 556, 694
Bst4CI ACNGT 1 cut(s) 246
Bst6I CTCTTC 1 cut(s) 177
BstBAI YACGTR 3 cut(s) 20, 379, 489
BstC8I GCNNGC 2 cut(s) 27, 37
BstDEI CTNAG 1 cut(s) 711
BstDSI CCRYGG 1 cut(s) 472
BstF5I GGATG 2 cut(s) 291, 577
BstFNI CGCG 1 cut(s) 237
BstKTI GATC 2 cut(s) 559, 697
BstMAI GTCTC 1 cut(s) 189
BstMBI GATC 2 cut(s) 556, 694
BstMWI GCNNNNNNNGC 3 cut(s) 53, 420, 617
BstNSI RCATGY 1 cut(s) 39
BstSCI CCNGG 3 cut(s) 414, 499, 534
BstSFI CTRYAG 1 cut(s) 101
BstUI CGCG 1 cut(s) 237
BstV2I GAAGAC 1 cut(s) 344
BstX2I RGATCY 1 cut(s) 556
BstXI CCANNNNNNTGG 1 cut(s) 568
BstYI RGATCY 1 cut(s) 556
Bsu15I ATCGAT 1 cut(s) 432
BsuRI GGCC 4 cut(s) 321, 414, 471, 542
BsuTUI ATCGAT 1 cut(s) 432
BtgI CCRYGG 1 cut(s) 472
BtrI CACGTC 1 cut(s) 616
BtsCI GGATG 2 cut(s) 291, 577
BtsIMutI CAGTG 1 cut(s) 212
BtuMI TCGCGA 1 cut(s) 237
Cac8I GCNNGC 2 cut(s) 27, 37
Cfr13I GGNCC 6 cut(s) 10, 319, 412, 469, 497, 526
ClaI ATCGAT 1 cut(s) 432
Csp6I GTAC 2 cut(s) 376, 484
CviAII CATG 4 cut(s) 36, 112, 508, 726
CviQI GTAC 2 cut(s) 376, 484
DdeI CTNAG 1 cut(s) 711
DpnI GATC 2 cut(s) 558, 696
DpnII GATC 2 cut(s) 556, 694
DraIII CACNNNGTG 2 cut(s) 20, 89
DrdI GACNNNNNNGTC 1 cut(s) 287
DseDI GACNNNNNNGTC 1 cut(s) 287
EaeI YGGCCR 1 cut(s) 540
Eam1104I CTCTTC 1 cut(s) 177
EarI CTCTTC 1 cut(s) 177
EciI GGCGGA 2 cut(s) 527, 635
Ecl136II GAGCTC 1 cut(s) 346
Eco24I GRGCYC 1 cut(s) 348
Eco47I GGWCC 3 cut(s) 10, 497, 526
Eco53kI GAGCTC 1 cut(s) 346
Eco72I CACGTG 2 cut(s) 20, 489
EcoICRI GAGCTC 1 cut(s) 346
EcoO109I RGGNCCY 2 cut(s) 10, 497
EcoT22I ATGCAT 1 cut(s) 37
EcoT38I GRGCYC 1 cut(s) 348
Esp3I CGTCTC 1 cut(s) 189
FaeI CATG 4 cut(s) 39, 115, 511, 729
FaiI YATR 9 cut(s) 37, 113, 178, 180, 182, 509, 545, 567, 727
FaqI GGGAC 3 cut(s) 23, 510, 518
FatI CATG 4 cut(s) 35, 111, 507, 725
FauI CCCGC 2 cut(s) 362, 470
FblI GTMKAC 1 cut(s) 396
Fnu4HI GCNGC 2 cut(s) 458, 540
FokI GGATG 2 cut(s) 278, 584
FriOI GRGCYC 1 cut(s) 348
Fsp4HI GCNGC 2 cut(s) 458, 540
FspBI CTAG 2 cut(s) 141, 681
GluI GCNGC 2 cut(s) 458, 540
HaeIII GGCC 4 cut(s) 321, 414, 471, 542
HapII CCGG 5 cut(s) 151, 322, 415, 501, 536
Hin1II CATG 4 cut(s) 39, 115, 511, 729
HindIII AAGCTT 1 cut(s) 127
HinfI GANTC 4 cut(s) 94, 200, 239, 444
HpaII CCGG 5 cut(s) 151, 322, 415, 501, 536
HphI GGTGA 3 cut(s) 124, 313, 688
Hpy166II GTNNAC 3 cut(s) 397, 486, 723
Hpy188I TCNGA 3 cut(s) 162, 296, 449
Hpy188III TCNNGA 2 cut(s) 236, 253
Hpy8I GTNNAC 3 cut(s) 397, 486, 723
Hpy99I CGWCG 1 cut(s) 272
HpyAV CCTTC 3 cut(s) 157, 592, 646
HpyCH4III ACNGT 1 cut(s) 246
HpyCH4IV ACGT 5 cut(s) 19, 88, 378, 488, 615
HpyCH4V TGCA 3 cut(s) 35, 39, 409
HpyF10VI GCNNNNNNNGC 3 cut(s) 53, 420, 617
HpyF3I CTNAG 1 cut(s) 711
HpySE526I ACGT 5 cut(s) 19, 88, 378, 488, 615
Hsp92II CATG 4 cut(s) 39, 115, 511, 729
KflI GGGWCCC 2 cut(s) 10, 497
Kzo9I GATC 2 cut(s) 556, 694
LguI GCTCTTC 1 cut(s) 177
LmnI GCTCC 1 cut(s) 343
LweI GCATC 1 cut(s) 22
MaeI CTAG 2 cut(s) 141, 681
MaeII ACGT 5 cut(s) 19, 88, 378, 488, 615
MaeIII GTNAC 2 cut(s) 79, 676
MalI GATC 2 cut(s) 558, 696
MbiI CCGCTC 1 cut(s) 299
MboI GATC 2 cut(s) 556, 694
MboII GAAGA 6 cut(s) 85, 89, 164, 337, 349, 397
MflI RGATCY 1 cut(s) 556
MhlI GDGCHC 2 cut(s) 348, 712
MlyI GAGTC 1 cut(s) 194
Mph1103I ATGCAT 1 cut(s) 37
MseI TTAA 1 cut(s) 248
MslI CAYNNNNRTG 1 cut(s) 40
MspI CCGG 5 cut(s) 151, 322, 415, 501, 536
MspR9I CCNGG 3 cut(s) 416, 501, 536
MvnI CGCG 1 cut(s) 237
MwoI GCNNNNNNNGC 3 cut(s) 53, 420, 617
NciI CCSGG 3 cut(s) 416, 501, 536
NdeII GATC 2 cut(s) 556, 694
NlaIII CATG 4 cut(s) 39, 115, 511, 729
NlaIV GGNNCC 6 cut(s) 11, 12, 498, 499, 528, 558
NmuCI GTSAC 1 cut(s) 676
NruI TCGCGA 1 cut(s) 237
NsiI ATGCAT 1 cut(s) 37
NspI RCATGY 1 cut(s) 39
PaeI GCATGC 1 cut(s) 39
PciSI GCTCTTC 1 cut(s) 177
PfeI GAWTC 3 cut(s) 94, 239, 444
PflFI GACNNNGTC 1 cut(s) 530
PflMI CCANNNNNTGG 1 cut(s) 20
PkrI GCNGC 2 cut(s) 459, 541
PleI GAGTC 1 cut(s) 194
PmaCI CACGTG 2 cut(s) 20, 489
PmlI CACGTG 2 cut(s) 20, 489
PpsI GAGTC 1 cut(s) 194
Ppu21I YACGTR 3 cut(s) 20, 379, 489
PpuMI RGGWCCY 2 cut(s) 10, 497
Psp124BI GAGCTC 1 cut(s) 348
Psp5II RGGWCCY 2 cut(s) 10, 497
PspCI CACGTG 2 cut(s) 20, 489
PspN4I GGNNCC 6 cut(s) 11, 12, 498, 499, 528, 558
PspPI GGNCC 6 cut(s) 10, 319, 412, 469, 497, 526
PspPPI RGGWCCY 2 cut(s) 10, 497
PsuI RGATCY 1 cut(s) 556
PsyI GACNNNGTC 1 cut(s) 530
RruI TCGCGA 1 cut(s) 237
RsaI GTAC 2 cut(s) 377, 485
RsaNI GTAC 2 cut(s) 376, 484
RseI CAYNNNNRTG 1 cut(s) 40
SacI GAGCTC 1 cut(s) 348
SapI GCTCTTC 1 cut(s) 177
SaqAI TTAA 1 cut(s) 248
SatI GCNGC 2 cut(s) 458, 540
Sau3AI GATC 2 cut(s) 556, 694
Sau96I GGNCC 6 cut(s) 10, 319, 412, 469, 497, 526
SchI GAGTC 1 cut(s) 194
ScrFI CCNGG 3 cut(s) 416, 501, 536
SduI GDGCHC 2 cut(s) 348, 712
SfaNI GCATC 1 cut(s) 22
SfcI CTRYAG 1 cut(s) 101
SinI GGWCC 3 cut(s) 10, 497, 526
SmiMI CAYNNNNRTG 1 cut(s) 40
SphI GCATGC 1 cut(s) 39
SsiI CCGC 9 cut(s) 299, 355, 455, 458, 477, 512, 539, 620, 656
SspMI CTAG 2 cut(s) 141, 681
SstI GAGCTC 1 cut(s) 348
StyD4I CCNGG 3 cut(s) 414, 499, 534
TaaI ACNGT 1 cut(s) 246
TaiI ACGT 5 cut(s) 22, 91, 381, 491, 618
TaqI TCGA 4 cut(s) 124, 267, 363, 432
TatI WGTACW 1 cut(s) 483
TauI GCSGC 2 cut(s) 460, 542
TfiI GAWTC 3 cut(s) 94, 239, 444
Tru1I TTAA 1 cut(s) 248
Tru9I TTAA 1 cut(s) 248
TscAI CASTG 1 cut(s) 219
TseFI GTSAC 1 cut(s) 676
Tsp45I GTSAC 1 cut(s) 676
TspDTI ATGAA 1 cut(s) 272
TspGWI ACGGA 1 cut(s) 356
TspRI CASTG 1 cut(s) 219
Tth111I GACNNNGTC 1 cut(s) 530
Van91I CCANNNNNTGG 1 cut(s) 20
VpaK11BI GGWCC 3 cut(s) 10, 497, 526
XceI RCATGY 1 cut(s) 39
XcmI CCANNNNNNNNNTGG 3 cut(s) 376, 431, 436
XmiI GTMKAC 1 cut(s) 396
XspI CTAG 2 cut(s) 141, 681
Zsp2I ATGCAT 1 cut(s) 37
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.