AT3G10820

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity). May play a role in transcription elongation (By similarity)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0010201
Physical Location & Seq
Forward (+)
3387078 .. 3391003
3926 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G10820.1

Sequence Viewer

Length: 1185 bp
ATGGCGAAGATGAGACCATCTGTTTCGTTGGATACTTGGAGAGAGTATTTCCGGCGAGGAGATTCTGATATTTTTGGTATCATTGATCATGCGATTATGGTAGCTGCTGCAGATTGGCCAAAGGAGTTTAAATCCAGGAGTGATAGAATTGCTGAGCTTCTCTTCTCTTGTAAAGTGAGTCGCTGCATTGGTTGTGACCACCTTGAGCTCTCGATAGCTGGAGACGAAGCGGCGGTGGAGATAGTTGGAGTTGGCGGCGGCGGTGATAGGGGTGATTCTGGTGTTGCGACGGGTGAAGGTGAAGAGGCGTCTGTGAGTGTTGATGAGGTTATGAGGATCAGAGATATCTTGTCGAACAAAGACGATGAGAAAGATTCTGTGTTACTCGAATCTCTGAGAAAGCTTGAGTCAATGTCTATGAGTGTGGACATTCTTAAGGATACTGAAATTGGAAAGGCTGTTAATGGTCTGAGGAGACATAGTTCCGATAAGATTAGCAAACTTGCAAAGACTCTTTTCGCAGAGTGGAAGAGGCTGGTGGACCAGTGGATGAACACCCCAGAGGAAATGGCTGGCACTGAAGGGACGCCAGAGTCTCTAAACCTTTCAGTAATTGATGAAGAAGAAGCCTTTCCTTCTCCTCCACATGATTTAGATATCTATGCACCTGAACCTAATGGGTTTGAACTTTCTCAGATCTTAGATTGTTTGGACTGCGATGGAAATCCTCGTCACAGTGTGGAGTCCAAACATGAAAGAAAATCGCAAAGTAGTGCGGGGAGGAGACCCAAGGGAACAAATGATGCAAATGTGGTTGGGAGGTACTGCAATGATCAACAGACGAGGAGAGAAGAAGCTGATGTTAGACCTATGAAGCACTCAGCCACAGATGTTGTCGAGCCTAAAAGACAAACAAAGCAAAGTAGAGAACAAATGGTATCCGCAATCCAACGAAAACCAACTGCTGTTACTGAGCAAAAGCGGAAACTTGCTGGACCTCAACAAGATAAACTCAAAGCTCTGGATCCAGACTCAAAGTTTGAGTTTGCAAAGCGGAAACTCCAAGAGAGCTACCACCAACATGAGAATGCTAAGAGACAGAGGACGATACAAGTACTGGAAACTATCCCAAAGCAAAATAAAGTTCAGAAACCGCAACTCAAGAGACCCGCCACACGAAGATAG

Protein Analysis

394

Amino Acids

44.31

Weight (kDa)

6.36

Isoelectric Point (pI)

54.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med26 PF08711 131 - 180 1e-16 TFIIS helical bundle-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 592
AclWI GGATC 3 cut(s) 344, 1019, 1032
AcoI YGGCCR 1 cut(s) 116
AcuI CTGAAG 1 cut(s) 600
AcyI GRCGYC 2 cut(s) 308, 587
AdeI CACNNNGTG 1 cut(s) 739
AfaI GTAC 2 cut(s) 824, 1116
AflII CTTAAG 1 cut(s) 434
AgsI TTSAA 1 cut(s) 686
AjnI CCWGG 1 cut(s) 134
AloI GAACNNNNNNTCC 2 cut(s) 466, 498
AluBI AGCT 8 cut(s) 104, 157, 208, 218, 403, 857, 1019, 1071
AluI AGCT 8 cut(s) 104, 157, 208, 218, 403, 857, 1019, 1071
Alw21I GWGCWC 1 cut(s) 210
Alw26I GTCTC 7 cut(s) 7, 216, 469, 600, 778, 1090, 1159
AlwI GGATC 3 cut(s) 344, 1019, 1032
AoxI GGCC 1 cut(s) 116
ApeKI GCWGC 3 cut(s) 104, 107, 183
ArsI GACNNNNNNTTYG 2 cut(s) 1022, 1054
Asp700I GAANNNNTTC 1 cut(s) 630
AspS9I GGNCC 2 cut(s) 541, 995
AsuHPI GGTGA 4 cut(s) 275, 284, 305, 311
AvaII GGWCC 2 cut(s) 541, 995
BalI TGGCCA 1 cut(s) 118
BamHI GGATCC 1 cut(s) 1024
BanII GRGCYC 1 cut(s) 210
Bbv12I GWGCWC 1 cut(s) 210
BbvI GCAGC 3 cut(s) 91, 94, 170
BccI CCATC 2 cut(s) 25, 713
BciT130I CCWGG 1 cut(s) 136
BciVI GTATCC 3 cut(s) 25, 433, 949
BclI TGATCA 2 cut(s) 85, 832
BcoDI GTCTC 7 cut(s) 7, 216, 469, 600, 778, 1090, 1159
BfmI CTRYAG 1 cut(s) 108
BfrI CTTAAG 1 cut(s) 434
BfuI GTATCC 3 cut(s) 25, 433, 949
BglII AGATCT 1 cut(s) 696
BisI GCNGC 6 cut(s) 105, 108, 184, 231, 256, 259
BlpI GCTNAGC 1 cut(s) 153
BlsI GCNGC 6 cut(s) 106, 109, 185, 232, 257, 260
BmcAI AGTACT 1 cut(s) 1116
Bme1390I CCNGG 1 cut(s) 136
Bme18I GGWCC 2 cut(s) 541, 995
BmgT120I GGNCC 2 cut(s) 541, 995
BmiI GGNNCC 1 cut(s) 1026
BmrFI CCNGG 1 cut(s) 136
BmsI GCATC 1 cut(s) 793
BpmI CTGGAG 1 cut(s) 240
Bpu1102I GCTNAGC 1 cut(s) 153
BpuEI CTTGAG 3 cut(s) 224, 425, 1145
BsaBI GATNNNNATC 1 cut(s) 723
BsaHI GRCGYC 2 cut(s) 308, 587
BsaI GGTCTC 3 cut(s) 7, 778, 1159
BsaJI CCNNGG 1 cut(s) 789
BsaXI ACNNNNNCTCC 2 cut(s) 466, 496
Bse1I ACTGG 2 cut(s) 544, 1122
Bse3DI GCAATG 1 cut(s) 835
Bse8I GATNNNNATC 1 cut(s) 723
BseBI CCWGG 1 cut(s) 136
BseDI CCNNGG 1 cut(s) 789
BseGI GGATG 1 cut(s) 555
BseJI GATNNNNATC 1 cut(s) 723
BseMI GCAATG 1 cut(s) 835
BseMII CTCAG 6 cut(s) 144, 386, 461, 707, 894, 963
BseNI ACTGG 2 cut(s) 544, 1122
BseRI GAGGAG 5 cut(s) 72, 487, 630, 796, 859
BseXI GCAGC 3 cut(s) 91, 94, 170
BshFI GGCC 1 cut(s) 118
BsiHKAI GWGCWC 1 cut(s) 210
BsiSI CCGG 1 cut(s) 52
BslFI GGGAC 1 cut(s) 598
BsmAI GTCTC 7 cut(s) 7, 216, 469, 600, 778, 1090, 1159
BsmBI CGTCTC 1 cut(s) 216
BsmFI GGGAC 1 cut(s) 598
BsmI GAATGC 1 cut(s) 1093
BsnI GGCC 1 cut(s) 118
Bso31I GGTCTC 3 cut(s) 7, 778, 1159
Bsp1286I GDGCHC 1 cut(s) 210
Bsp143I GATC 5 cut(s) 85, 336, 696, 832, 1024
Bsp1720I GCTNAGC 1 cut(s) 153
BspANI GGCC 1 cut(s) 118
BspCNI CTCAG 6 cut(s) 145, 387, 462, 706, 893, 964
BspLI GGNNCC 1 cut(s) 1026
BspMAI CTGCAG 1 cut(s) 112
BspPI GGATC 3 cut(s) 344, 1019, 1032
BspTI CTTAAG 1 cut(s) 434
BspTNI GGTCTC 3 cut(s) 7, 778, 1159
BsrDI GCAATG 1 cut(s) 835
BsrI ACTGG 2 cut(s) 544, 1122
BssECI CCNNGG 1 cut(s) 789
BssMI GATC 5 cut(s) 85, 336, 696, 832, 1024
BssNI GRCGYC 2 cut(s) 308, 587
BssT1I CCWWGG 1 cut(s) 789
Bst2UI CCWGG 1 cut(s) 136
Bst4CI ACNGT 1 cut(s) 737
Bst6I CTCTTC 3 cut(s) 167, 297, 524
BstACI GRCGYC 2 cut(s) 308, 587
BstAFI CTTAAG 1 cut(s) 434
BstC8I GCNNGC 1 cut(s) 574
BstDEI CTNAG 8 cut(s) 153, 395, 470, 693, 700, 880, 972, 1092
BstF5I GGATG 1 cut(s) 555
BstKTI GATC 5 cut(s) 88, 339, 699, 835, 1027
BstMAI GTCTC 7 cut(s) 7, 216, 469, 600, 778, 1090, 1159
BstMBI GATC 5 cut(s) 85, 336, 696, 832, 1024
BstNI CCWGG 1 cut(s) 136
BstSCI CCNGG 1 cut(s) 134
BstSFI CTRYAG 1 cut(s) 108
BstV1I GCAGC 3 cut(s) 91, 94, 170
BstX2I RGATCY 2 cut(s) 696, 1024
BstYI RGATCY 2 cut(s) 696, 1024
BsuI GTATCC 3 cut(s) 25, 433, 949
BsuRI GGCC 1 cut(s) 118
BtgZI GCGATG 1 cut(s) 732
BtsCI GGATG 1 cut(s) 555
BtsIMutI CAGTG 3 cut(s) 551, 576, 742
Cac8I GCNNGC 1 cut(s) 574
Cfr13I GGNCC 2 cut(s) 541, 995
CseI GACGC 2 cut(s) 297, 595
Csp6I GTAC 2 cut(s) 823, 1115
CspCI CAANNNNNGTGG 2 cut(s) 874, 909
CviAII CATG 4 cut(s) 89, 647, 752, 1082
CviQI GTAC 2 cut(s) 823, 1115
DdeI CTNAG 8 cut(s) 153, 395, 470, 693, 700, 880, 972, 1092
DpnI GATC 5 cut(s) 87, 338, 698, 834, 1026
DpnII GATC 5 cut(s) 85, 336, 696, 832, 1024
DraI TTTAAA 1 cut(s) 130
DraIII CACNNNGTG 1 cut(s) 739
DrdI GACNNNNNNGTC 1 cut(s) 592
DseDI GACNNNNNNGTC 1 cut(s) 592
EaeI YGGCCR 1 cut(s) 116
Eam1104I CTCTTC 3 cut(s) 167, 297, 524
EarI CTCTTC 3 cut(s) 167, 297, 524
Ecl136II GAGCTC 1 cut(s) 208
Eco130I CCWWGG 1 cut(s) 789
Eco24I GRGCYC 1 cut(s) 210
Eco31I GGTCTC 3 cut(s) 7, 778, 1159
Eco32I GATATC 2 cut(s) 346, 658
Eco47I GGWCC 2 cut(s) 541, 995
Eco53kI GAGCTC 1 cut(s) 208
Eco57I CTGAAG 1 cut(s) 600
EcoICRI GAGCTC 1 cut(s) 208
EcoRII CCWGG 1 cut(s) 134
EcoRV GATATC 2 cut(s) 346, 658
EcoT14I CCWWGG 1 cut(s) 789
EcoT38I GRGCYC 1 cut(s) 210
ErhI CCWWGG 1 cut(s) 789
Esp3I CGTCTC 1 cut(s) 216
FaeI CATG 4 cut(s) 92, 650, 755, 1085
FaqI GGGAC 1 cut(s) 598
FatI CATG 4 cut(s) 88, 646, 751, 1081
FauI CCCGC 2 cut(s) 769, 1177
FbaI TGATCA 2 cut(s) 85, 832
Fnu4HI GCNGC 6 cut(s) 105, 108, 184, 231, 256, 259
FokI GGATG 1 cut(s) 562
FriOI GRGCYC 1 cut(s) 210
Fsp4HI GCNGC 6 cut(s) 105, 108, 184, 231, 256, 259
GluI GCNGC 6 cut(s) 105, 108, 184, 231, 256, 259
GsuI CTGGAG 1 cut(s) 240
HaeIII GGCC 1 cut(s) 118
HapII CCGG 1 cut(s) 52
HgaI GACGC 2 cut(s) 297, 595
Hin1I GRCGYC 2 cut(s) 308, 587
Hin1II CATG 4 cut(s) 92, 650, 755, 1085
HindIII AAGCTT 1 cut(s) 401
HpaII CCGG 1 cut(s) 52
HphI GGTGA 4 cut(s) 275, 284, 305, 311
Hpy166II GTNNAC 2 cut(s) 427, 541
Hpy188I TCNGA 7 cut(s) 67, 341, 396, 471, 487, 696, 1149
Hpy188III TCNNGA 4 cut(s) 211, 1022, 1028, 1162
Hpy8I GTNNAC 2 cut(s) 427, 541
Hpy99I CGWCG 1 cut(s) 292
HpyAV CCTTC 3 cut(s) 290, 575, 645
HpyCH4III ACNGT 1 cut(s) 737
HpyCH4V TGCA 7 cut(s) 110, 186, 506, 665, 806, 828, 1049
HpyF3I CTNAG 8 cut(s) 153, 395, 470, 693, 700, 880, 972, 1092
Hsp92I GRCGYC 2 cut(s) 308, 587
Hsp92II CATG 4 cut(s) 92, 650, 755, 1085
Ksp22I TGATCA 2 cut(s) 85, 832
Kzo9I GATC 5 cut(s) 85, 336, 696, 832, 1024
Lsp1109I GCAGC 3 cut(s) 91, 94, 170
LweI GCATC 1 cut(s) 793
MaeIII GTNAC 4 cut(s) 194, 381, 731, 967
MalI GATC 5 cut(s) 87, 338, 698, 834, 1026
MboI GATC 5 cut(s) 85, 336, 696, 832, 1024
MboII GAAGA 7 cut(s) 19, 154, 314, 541, 632, 635, 863
MflI RGATCY 2 cut(s) 696, 1024
MhlI GDGCHC 1 cut(s) 210
MlsI TGGCCA 1 cut(s) 118
MluCI AATT 3 cut(s) 147, 447, 612
MluNI TGGCCA 1 cut(s) 118
MlyI GAGTC 6 cut(s) 187, 416, 505, 602, 752, 1025
MmeI TCCRAC 3 cut(s) 9, 226, 973
Mox20I TGGCCA 1 cut(s) 118
MroXI GAANNNNTTC 1 cut(s) 630
MscI TGGCCA 1 cut(s) 118
MseI TTAA 3 cut(s) 129, 435, 462
MslI CAYNNNNRTG 2 cut(s) 1080, 1086
Msp20I TGGCCA 1 cut(s) 118
MspCI CTTAAG 1 cut(s) 434
MspI CCGG 1 cut(s) 52
MspR9I CCNGG 1 cut(s) 136
Mva1269I GAATGC 1 cut(s) 1093
MvaI CCWGG 1 cut(s) 136
NdeII GATC 5 cut(s) 85, 336, 696, 832, 1024
NlaIII CATG 4 cut(s) 92, 650, 755, 1085
NlaIV GGNNCC 1 cut(s) 1026
NmuCI GTSAC 2 cut(s) 194, 731
PctI GAATGC 1 cut(s) 1093
PdmI GAANNNNTTC 1 cut(s) 630
PfeI GAWTC 4 cut(s) 62, 275, 374, 389
PfoI TCCNGGA 1 cut(s) 134
PkrI GCNGC 6 cut(s) 106, 109, 185, 232, 257, 260
PleI GAGTC 6 cut(s) 186, 415, 505, 601, 751, 1025
PpsI GAGTC 6 cut(s) 186, 415, 505, 601, 751, 1025
Psp124BI GAGCTC 1 cut(s) 210
Psp6I CCWGG 1 cut(s) 134
PspGI CCWGG 1 cut(s) 134
PspN4I GGNNCC 1 cut(s) 1026
PspPI GGNCC 2 cut(s) 541, 995
PstI CTGCAG 1 cut(s) 112
PsuI RGATCY 2 cut(s) 696, 1024
RsaI GTAC 2 cut(s) 824, 1116
RsaNI GTAC 2 cut(s) 823, 1115
RseI CAYNNNNRTG 2 cut(s) 1080, 1086
SacI GAGCTC 1 cut(s) 210
SaqAI TTAA 3 cut(s) 129, 435, 462
SatI GCNGC 6 cut(s) 105, 108, 184, 231, 256, 259
Sau3AI GATC 5 cut(s) 85, 336, 696, 832, 1024
Sau96I GGNCC 2 cut(s) 541, 995
ScaI AGTACT 1 cut(s) 1116
SchI GAGTC 6 cut(s) 187, 416, 505, 602, 752, 1025
ScrFI CCNGG 1 cut(s) 136
SduI GDGCHC 1 cut(s) 210
SfaNI GCATC 1 cut(s) 793
SfcI CTRYAG 1 cut(s) 108
SinI GGWCC 2 cut(s) 541, 995
SmiMI CAYNNNNRTG 2 cut(s) 1080, 1086
SmlI CTYRAG 4 cut(s) 203, 404, 434, 1160
SmoI CTYRAG 4 cut(s) 203, 404, 434, 1160
Sse9I AATT 3 cut(s) 147, 447, 612
SstI GAGCTC 1 cut(s) 210
StyD4I CCNGG 1 cut(s) 134
StyI CCWWGG 1 cut(s) 789
TaaI ACNGT 1 cut(s) 737
TaqI TCGA 4 cut(s) 212, 353, 387, 897
TasI AATT 3 cut(s) 147, 447, 612
TatI WGTACW 1 cut(s) 1114
TauI GCSGC 3 cut(s) 233, 258, 261
TfiI GAWTC 4 cut(s) 62, 275, 374, 389
Tru1I TTAA 3 cut(s) 129, 435, 462
Tru9I TTAA 3 cut(s) 129, 435, 462
TscAI CASTG 3 cut(s) 551, 583, 742
TseFI GTSAC 2 cut(s) 194, 731
TseI GCWGC 3 cut(s) 104, 107, 183
Tsp45I GTSAC 2 cut(s) 194, 731
TspDTI ATGAA 4 cut(s) 566, 633, 768, 887
TspRI CASTG 3 cut(s) 551, 583, 742
Vha464I CTTAAG 1 cut(s) 434
VpaK11BI GGWCC 2 cut(s) 541, 995
XmnI GAANNNNTTC 1 cut(s) 630
ZrmI AGTACT 1 cut(s) 1116
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.