FvH4_6g37520

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0010201
Physical Location & Seq
Forward (+)
29553803 .. 29557415
3613 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g37520.t1

Sequence Viewer

Length: 1377 bp
ATGGCGACCACGGCCAAGTCTTCTGGGTCTTCTTCTTCGCTGAATTACTGGCGGAGCTATTTCCGGACGGCGAATTCGGACATTTTCGGCATCATTGACCATGCTATTATGGTCGCGGCGGTGGATTGTCCCAAGGAGTTCCGGCTGCGGAGGGATCGGGTGGCTGAGCTGTTGTTTTCTTGCCGGATGAGCCGGTGTTCGGGTTGCGAAAAGGTTGAGCTGGTGGTGCCTTGTGATCATGGTGAAGGTGATGAGGGTGTGGACGGTAGTGGTGGGGATGGAGATTTTGGAGGTGGGGCTAGTAAAGAGAGCAAGGTCAATAGCACCAGTAGGGATGATGATCAGGTGATGAAGAATGATAGCAATTACGGATTGGCTGAGGCTTTGACTGATGAGATTGAAGAAGAGTCTCAGGTTTTCGAGGAGGTTCTGAGGATCAAGGAGGTCTTCCAGAATAGTGAGCATGAGTCTGATGCTGTGTTGTATGAGTCATTGAGGAAGCTTGAGTTGATGGCTCTGTCTGTGGAGACTCTCAAGGCAACTGAGATTGGGAAGGCGGTGAACCGATTAAGAAAGCATGGATCAGTGAAGATTCGTAATCTGGTTCGAGCACTGATTGATGGGTGGAAAGTTATGGTGGATGAGTGGGTGAATGCTACTACAGCTATTGCTTCTGGGGGAACTGAGGTTATTTCGGACTGTGTTGATCCATCGGTTGATTATGACTTTGAGGAAGGACTCCCTACTCCTCCATTGGATGAAGGAGCTTTCTTTGCTACTCAAACCACCTCAATGGAACTGTCTGAGTTCTTTGATGGTATGGAAGATTATGGGAACCCTCGAAACAATGGGCAATACACGAGGAATCGTGAGAATGGAAGAAAACCCTCATTGGAGAAACAAAAGTTTGTGAAGCAGGAACAACAGTATGAAAGTGATGAGGGGAATGTGCTTCCGAGGAAAATAAAGAGTGAACCAGTGATGGTGAAACAAGAAGTTGATGTGAAGCCAAATAGACCCTCAAACACCAACTCCGGCCCTCGCAGACCTCCCAAAATAACTATGGAGCAAAAAGGTGTCAGTAGTGTAACGAATATTGAACAAAGAACAGAAAAGTCTTCTATTCAGAAGAGGCAAGTCACTGGTCAGCAGCAAGATAAATATAAGTGTTCAGATGAAGTTGCAGTCCAAGTGAAGCTTGAAGCTACAAAAAGGAAACTCCAGGAGAGCTATCAACAAGCTGAGAATGCCAAGAAGCAACGGACTGTACAGGTAATGGAATTGCATGATCTTCCCAAGCAGGGGCTTGGCCATAGGAATCCACATGTGAAACATGGAAACCACATTCGACAAAGGGCTCTTGGGCGGAGATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Med26

Protein Analysis

459

Amino Acids

51.43

Weight (kDa)

5.87

Isoelectric Point (pI)

41.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med26 PF08711 164 - 213 1.2e-13 TFIIS helical bundle-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 226
AccII CGCG 1 cut(s) 116
AccIII TCCGGA 1 cut(s) 63
AciI CCGC 6 cut(s) 52, 116, 119, 148, 557, 1366
AclWI GGATC 4 cut(s) 162, 443, 589, 701
AcoI YGGCCR 2 cut(s) 12, 1309
AcsI RAATTY 1 cut(s) 73
AfaI GTAC 1 cut(s) 1269
AfiI CCNNNNNNNGG 3 cut(s) 199, 1301, 1302
AflIII ACRYGT 1 cut(s) 1324
AgsI TTSAA 3 cut(s) 401, 1100, 1202
AjnI CCWGG 1 cut(s) 1221
Alw21I GWGCWC 1 cut(s) 613
Alw26I GTCTC 2 cut(s) 414, 521
AlwI GGATC 4 cut(s) 162, 443, 589, 701
Aor13HI TCCGGA 1 cut(s) 63
AoxI GGCC 3 cut(s) 12, 1036, 1309
ApeKI GCWGC 2 cut(s) 145, 1150
ApoI RAATTY 1 cut(s) 73
ArsI GACNNNNNNTTYG 2 cut(s) 58, 90
AspS9I GGNCC 1 cut(s) 1037
AsuHPI GGTGA 6 cut(s) 254, 260, 358, 571, 661, 997
BalI TGGCCA 1 cut(s) 1311
BanI GGYRCC 1 cut(s) 226
BanII GRGCYC 1 cut(s) 1360
BauI CACGAG 1 cut(s) 859
BbsI GAAGAC 4 cut(s) 12, 21, 439, 1110
Bbv12I GWGCWC 1 cut(s) 613
BbvCI CCTCAGC 1 cut(s) 378
BbvI GCAGC 2 cut(s) 132, 1162
BccI CCATC 6 cut(s) 272, 505, 614, 718, 809, 976
BceAI ACGGC 2 cut(s) 27, 84
BciT130I CCWGG 1 cut(s) 1223
BclI TGATCA 2 cut(s) 235, 340
BcoDI GTCTC 2 cut(s) 414, 521
BfaI CTAG 1 cut(s) 300
BfmI CTRYAG 1 cut(s) 660
BisI GCNGC 3 cut(s) 117, 146, 1151
BlpI GCTNAGC 1 cut(s) 165
BlsI GCNGC 3 cut(s) 118, 147, 1152
Bme1390I CCNGG 1 cut(s) 1223
BmgT120I GGNCC 1 cut(s) 1037
BmiI GGNNCC 2 cut(s) 228, 836
BmrFI CCNGG 1 cut(s) 1223
BmsI GCATC 2 cut(s) 99, 463
BpiI GAAGAC 4 cut(s) 12, 21, 439, 1110
BpmI CTGGAG 1 cut(s) 1205
Bpu10I CCTNAGC 1 cut(s) 378
Bpu1102I GCTNAGC 1 cut(s) 165
BpuEI CTTGAG 2 cut(s) 518, 524
BsaBI GATNNNNATC 1 cut(s) 339
BsaJI CCNNGG 3 cut(s) 9, 132, 956
BsaWI WCCGGW 1 cut(s) 63
BsaXI ACNNNNNCTCC 2 cut(s) 1016, 1046
Bsc4I CCNNNNNNNGG 3 cut(s) 199, 1301, 1302
Bse118I RCCGGY 1 cut(s) 192
Bse1I ACTGG 4 cut(s) 53, 327, 977, 1147
Bse8I GATNNNNATC 1 cut(s) 339
BseAI TCCGGA 1 cut(s) 63
BseBI CCWGG 1 cut(s) 1223
BseDI CCNNGG 3 cut(s) 9, 132, 956
BseGI GGATG 5 cut(s) 192, 283, 340, 646, 763
BseJI GATNNNNATC 1 cut(s) 339
BseLI CCNNNNNNNGG 3 cut(s) 199, 1301, 1302
BseMII CTCAG 8 cut(s) 156, 369, 422, 425, 534, 675, 795, 1233
BseNI ACTGG 4 cut(s) 53, 327, 977, 1147
BseRI GAGGAG 2 cut(s) 437, 738
BseXI GCAGC 2 cut(s) 132, 1162
Bsh1236I CGCG 1 cut(s) 116
BshFI GGCC 3 cut(s) 14, 1038, 1311
BshNI GGYRCC 1 cut(s) 226
BsiHKAI GWGCWC 1 cut(s) 613
BsiSI CCGG 5 cut(s) 64, 142, 184, 193, 1035
BslFI GGGAC 1 cut(s) 114
BslI CCNNNNNNNGG 3 cut(s) 199, 1301, 1302
BsmAI GTCTC 2 cut(s) 414, 521
BsmFI GGGAC 1 cut(s) 114
BsmI GAATGC 2 cut(s) 658, 1252
BsnI GGCC 3 cut(s) 14, 1038, 1311
Bsp1286I GDGCHC 2 cut(s) 613, 1360
Bsp13I TCCGGA 1 cut(s) 63
Bsp1407I TGTACA 1 cut(s) 1267
Bsp143I GATC 7 cut(s) 154, 235, 340, 435, 581, 706, 1288
Bsp1720I GCTNAGC 1 cut(s) 165
BspACI CCGC 6 cut(s) 52, 116, 119, 148, 557, 1366
BspANI GGCC 3 cut(s) 14, 1038, 1311
BspCNI CTCAG 8 cut(s) 157, 370, 423, 424, 535, 676, 796, 1234
BspEI TCCGGA 1 cut(s) 63
BspFNI CGCG 1 cut(s) 116
BspLI GGNNCC 2 cut(s) 228, 836
BspPI GGATC 4 cut(s) 162, 443, 589, 701
BspT107I GGYRCC 1 cut(s) 226
BsrFI RCCGGY 1 cut(s) 192
BsrGI TGTACA 1 cut(s) 1267
BsrI ACTGG 4 cut(s) 53, 327, 977, 1147
BssAI RCCGGY 1 cut(s) 192
BssECI CCNNGG 3 cut(s) 9, 132, 956
BssMI GATC 7 cut(s) 154, 235, 340, 435, 581, 706, 1288
BssSI CACGAG 1 cut(s) 859
BssT1I CCWWGG 1 cut(s) 132
Bst2BI CACGAG 1 cut(s) 859
Bst2UI CCWGG 1 cut(s) 1223
Bst4CI ACNGT 5 cut(s) 266, 701, 801, 927, 1267
Bst6I CTCTTC 2 cut(s) 399, 1124
BstAUI TGTACA 1 cut(s) 1267
BstDEI CTNAG 8 cut(s) 165, 378, 411, 431, 543, 684, 804, 1242
BstDSI CCRYGG 1 cut(s) 9
BstF5I GGATG 5 cut(s) 192, 283, 340, 646, 763
BstFNI CGCG 1 cut(s) 116
BstKTI GATC 7 cut(s) 157, 238, 343, 438, 584, 709, 1291
BstMAI GTCTC 2 cut(s) 414, 521
BstMBI GATC 7 cut(s) 154, 235, 340, 435, 581, 706, 1288
BstMWI GCNNNNNNNGC 6 cut(s) 11, 189, 226, 662, 773, 1247
BstNI CCWGG 1 cut(s) 1223
BstNSI RCATGY 1 cut(s) 1328
BstSCI CCNGG 1 cut(s) 1221
BstSFI CTRYAG 1 cut(s) 660
BstUI CGCG 1 cut(s) 116
BstV1I GCAGC 2 cut(s) 132, 1162
BstV2I GAAGAC 4 cut(s) 12, 21, 439, 1110
BstXI CCANNNNNNTGG 1 cut(s) 793
BsuRI GGCC 3 cut(s) 14, 1038, 1311
BtgI CCRYGG 1 cut(s) 9
BtsCI GGATG 5 cut(s) 192, 283, 340, 646, 763
BtsIMutI CAGTG 4 cut(s) 591, 611, 984, 1140
Cfr10I RCCGGY 1 cut(s) 192
Cfr13I GGNCC 1 cut(s) 1037
Csp6I GTAC 1 cut(s) 1268
CviAII CATG 7 cut(s) 101, 239, 464, 578, 1286, 1325, 1334
CviQI GTAC 1 cut(s) 1268
DdeI CTNAG 8 cut(s) 165, 378, 411, 431, 543, 684, 804, 1242
DpnI GATC 7 cut(s) 156, 237, 342, 437, 583, 708, 1290
DpnII GATC 7 cut(s) 154, 235, 340, 435, 581, 706, 1288
EaeI YGGCCR 2 cut(s) 12, 1309
Eam1104I CTCTTC 2 cut(s) 399, 1124
EarI CTCTTC 2 cut(s) 399, 1124
EciI GGCGGA 1 cut(s) 67
Eco130I CCWWGG 1 cut(s) 132
Eco24I GRGCYC 1 cut(s) 1360
EcoRI GAATTC 1 cut(s) 73
EcoRII CCWGG 1 cut(s) 1221
EcoT14I CCWWGG 1 cut(s) 132
EcoT38I GRGCYC 1 cut(s) 1360
ErhI CCWWGG 1 cut(s) 132
FaeI CATG 7 cut(s) 104, 242, 467, 581, 1289, 1328, 1337
FalI AAGNNNNNCTT 4 cut(s) 431, 463, 1182, 1214
FaqI GGGAC 1 cut(s) 114
FatI CATG 7 cut(s) 100, 238, 463, 577, 1285, 1324, 1333
FbaI TGATCA 2 cut(s) 235, 340
Fnu4HI GCNGC 3 cut(s) 117, 146, 1151
FokI GGATG 5 cut(s) 199, 290, 347, 653, 770
FriOI GRGCYC 1 cut(s) 1360
Fsp4HI GCNGC 3 cut(s) 117, 146, 1151
FspBI CTAG 1 cut(s) 300
GluI GCNGC 3 cut(s) 117, 146, 1151
GsuI CTGGAG 1 cut(s) 1205
HaeIII GGCC 3 cut(s) 14, 1038, 1311
HapII CCGG 5 cut(s) 64, 142, 184, 193, 1035
Hin1II CATG 7 cut(s) 104, 242, 467, 581, 1289, 1328, 1337
HindIII AAGCTT 2 cut(s) 500, 1196
HinfI GANTC 9 cut(s) 407, 467, 488, 529, 592, 738, 865, 1318, 1371
HpaII CCGG 5 cut(s) 64, 142, 184, 193, 1035
HphI GGTGA 6 cut(s) 254, 260, 358, 571, 661, 997
Hpy166II GTNNAC 3 cut(s) 262, 562, 974
Hpy188I TCNGA 9 cut(s) 79, 432, 472, 697, 805, 957, 1128, 1174, 1376
Hpy188III TCNNGA 3 cut(s) 64, 451, 869
Hpy8I GTNNAC 3 cut(s) 262, 562, 974
HpyAV CCTTC 4 cut(s) 239, 547, 728, 755
HpyCH4III ACNGT 5 cut(s) 266, 701, 801, 927, 1267
HpyCH4V TGCA 2 cut(s) 1184, 1285
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 189, 226, 662, 773, 1247
HpyF3I CTNAG 8 cut(s) 165, 378, 411, 431, 543, 684, 804, 1242
Hsp92II CATG 7 cut(s) 104, 242, 467, 581, 1289, 1328, 1337
Kpn2I TCCGGA 1 cut(s) 63
Ksp22I TGATCA 2 cut(s) 235, 340
Kzo9I GATC 7 cut(s) 154, 235, 340, 435, 581, 706, 1288
LmnI GCTCC 3 cut(s) 54, 764, 1066
Lsp1109I GCAGC 2 cut(s) 132, 1162
LweI GCATC 2 cut(s) 99, 463
MaeI CTAG 1 cut(s) 300
MaeIII GTNAC 2 cut(s) 1087, 1138
MalI GATC 7 cut(s) 156, 237, 342, 437, 583, 708, 1290
MboI GATC 7 cut(s) 154, 235, 340, 435, 581, 706, 1288
MhlI GDGCHC 2 cut(s) 613, 1360
MlsI TGGCCA 1 cut(s) 1311
MluCI AATT 4 cut(s) 43, 73, 364, 1280
MluNI TGGCCA 1 cut(s) 1311
MlyI GAGTC 5 cut(s) 416, 476, 497, 523, 732
Mox20I TGGCCA 1 cut(s) 1311
MroI TCCGGA 1 cut(s) 63
MscI TGGCCA 1 cut(s) 1311
MseI TTAA 1 cut(s) 569
MslI CAYNNNNRTG 1 cut(s) 791
Msp20I TGGCCA 1 cut(s) 1311
MspI CCGG 5 cut(s) 64, 142, 184, 193, 1035
MspR9I CCNGG 1 cut(s) 1223
Mva1269I GAATGC 2 cut(s) 658, 1252
MvaI CCWGG 1 cut(s) 1223
MvnI CGCG 1 cut(s) 116
MwoI GCNNNNNNNGC 6 cut(s) 11, 189, 226, 662, 773, 1247
NdeII GATC 7 cut(s) 154, 235, 340, 435, 581, 706, 1288
NlaIII CATG 7 cut(s) 104, 242, 467, 581, 1289, 1328, 1337
NlaIV GGNNCC 2 cut(s) 228, 836
NmuCI GTSAC 1 cut(s) 1138
NspI RCATGY 1 cut(s) 1328
PciI ACATGT 1 cut(s) 1324
PctI GAATGC 2 cut(s) 658, 1252
PfeI GAWTC 4 cut(s) 592, 865, 1318, 1371
PfoI TCCNGGA 1 cut(s) 1221
PkrI GCNGC 3 cut(s) 118, 147, 1152
PleI GAGTC 5 cut(s) 415, 475, 496, 523, 732
PpsI GAGTC 5 cut(s) 415, 475, 496, 523, 732
PscI ACATGT 1 cut(s) 1324
Psp6I CCWGG 1 cut(s) 1221
PspGI CCWGG 1 cut(s) 1221
PspN4I GGNNCC 2 cut(s) 228, 836
PspPI GGNCC 1 cut(s) 1037
RsaI GTAC 1 cut(s) 1269
RsaNI GTAC 1 cut(s) 1268
RseI CAYNNNNRTG 1 cut(s) 791
SaqAI TTAA 1 cut(s) 569
SatI GCNGC 3 cut(s) 117, 146, 1151
Sau3AI GATC 7 cut(s) 154, 235, 340, 435, 581, 706, 1288
Sau96I GGNCC 1 cut(s) 1037
SchI GAGTC 5 cut(s) 416, 476, 497, 523, 732
ScrFI CCNGG 1 cut(s) 1223
SduI GDGCHC 2 cut(s) 613, 1360
SfaNI GCATC 2 cut(s) 99, 463
SfcI CTRYAG 1 cut(s) 660
SmiMI CAYNNNNRTG 1 cut(s) 791
SmlI CTYRAG 2 cut(s) 503, 533
SmoI CTYRAG 2 cut(s) 503, 533
Sse9I AATT 4 cut(s) 43, 73, 364, 1280
SsiI CCGC 6 cut(s) 52, 116, 119, 148, 557, 1366
SspI AATATT 1 cut(s) 1096
SspMI CTAG 1 cut(s) 300
StyD4I CCNGG 1 cut(s) 1221
StyI CCWWGG 1 cut(s) 132
TaaI ACNGT 5 cut(s) 266, 701, 801, 927, 1267
TaqI TCGA 4 cut(s) 420, 607, 841, 1348
TasI AATT 4 cut(s) 43, 73, 364, 1280
TatI WGTACW 1 cut(s) 1267
TauI GCSGC 1 cut(s) 119
TfiI GAWTC 4 cut(s) 592, 865, 1318, 1371
Tru1I TTAA 1 cut(s) 569
Tru9I TTAA 1 cut(s) 569
TscAI CASTG 4 cut(s) 591, 618, 984, 1147
TseFI GTSAC 1 cut(s) 1138
TseI GCWGC 2 cut(s) 145, 1150
Tsp45I GTSAC 1 cut(s) 1138
TspDTI ATGAA 4 cut(s) 365, 774, 945, 1191
TspGWI ACGGA 2 cut(s) 384, 1276
TspRI CASTG 4 cut(s) 591, 618, 984, 1147
XapI RAATTY 1 cut(s) 73
XceI RCATGY 1 cut(s) 1328
XcmI CCANNNNNNNNNTGG 1 cut(s) 1060
XspI CTAG 1 cut(s) 300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.