MD14G1088300.v1.1

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0010201
Physical Location & Seq
Forward (+)
11048652 .. 11052365
3714 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1088300.v1.1.491

Sequence Viewer

Length: 1368 bp
ATGGCGACCAACTCCGGGTCACTGAATTATTGGAGGGATTACTTTCGGACTGCAAACTCTGATATTTTTGATATAATCGACCATGCGATCGTGGTGGCGGCTTCCGATTGCCCCACGGAGTTGAGATTGCGGCGGGATCGGATAGCAGAGCGGTTGTTTTCCAGCAGATTGACCCGGTGGTGTTCGGGTTGCGAAAAACAAGTCGAATTGGCCACCGTGCCTGGCGGCGATGGAGAAGGAGATGACGACGAAGGCGGCAGCGACGATGTGCACAGAGGGTGTAAAAGTGGGTTTGATGGAGGTGGAGGGGAAGTCGAGGGCGGTGGCAGTAAAGAGAGCAAGGTGAATAGCAGCAGCAGAGATGATGAGGGCGGGCTTAGCAGCGACAGCTATGGATTGGCTGAGGCCTTGACTGATGAGATTGAGCAAGAGTCTCAAACTCTTGGGGAGGTCTTCAGGATTAGAGACATGTTTCTTGATTACGAACAAGAGTCTGATACGGTATTGTTTGACTCTTTGAGGAAGCTTCAGTTGATGGCTCTGAATGTGGAGACTCTCGAGGCAACTGAAATTGGAAAGGCGGTTAGTGGTCTCAAAAAGCATGGATCAAAGCAGATTCGTCATCTTGCTCGAGAGCTTGTCTCAGGTTGGAAAGGCATGGTAGATGAATGGGTCAATGCTACAGCAGCTATTGCCAGCACAAAAACTATGTCAGATTCTGTTAATCCATCTTTTGATGATGAAGAAGGACTACCATCTCCTCCTCTTGATGAAGCTTTCCTTGCTAATCAAACCACGACAATAGAACTCTCGCAGTTCTTTGACGGCATGGATGATGATGGAAATCCTCGAAACAACGGGGAATTCACTGAGTACCGTGAAAATGGAAGAAAGCGAGAAGTGGAGAAAGAAAATACTATTAAGCAGAACCAACAATATTCAAATGAGGCAAATGTGCTTCTTAAGGGAAACAAGAATGAACAAGTGAAGAAACGAGAAGTTGTGAAGCCAAATAAGCCCTCAAACACTCACTATGGGCCTGGCAGACCTCCCAAAGTTAGTATGGATCAAAAGCTCAACAGTGAAACAAAGATCGAGCAAAAAACAGATAAGGTTGCCATTCAAAAGAGGCAACACGCTGGTCAGCAAAATAAATTCAAGTGTTCAGATGAAGTTGCAGTCCAAGTGAAACTCGAAGCCACAAAAAGGAAGCTCCAAGAGCGTTATCAACAGGCTGAGAACGCCAAGAGACAAAGGACGGTACAGGTTATGGAGTTGCATGATCTCCCCAAGCAAGGGCTTGCGCGTAGGAATCCACACGGGAGACCTGGAAACCAAAATCGGCATTGGGCTCATGGACGGAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Med26

Protein Analysis

456

Amino Acids

50.96

Weight (kDa)

5.66

Isoelectric Point (pI)

43.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med26 PF08711 172 - 221 2.4e-13 TFIIS helical bundle-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 151
AccII CGCG 1 cut(s) 1306
AciI CCGC 9 cut(s) 98, 130, 133, 151, 225, 255, 321, 372, 581
AclWI GGATC 3 cut(s) 144, 613, 1074
AcoI YGGCCR 1 cut(s) 210
AcsI RAATTY 2 cut(s) 863, 1154
AcuI CTGAAG 2 cut(s) 439, 512
AfaI GTAC 2 cut(s) 875, 1263
AfiI CCNNNNNNNGG 4 cut(s) 15, 1206, 1295, 1296
AflII CTTAAG 1 cut(s) 962
AflIII ACRYGT 1 cut(s) 468
AgsI TTSAA 3 cut(s) 942, 1124, 1159
AjnI CCWGG 3 cut(s) 220, 1039, 1327
AluBI AGCT 7 cut(s) 390, 526, 637, 689, 776, 1075, 1213
AluI AGCT 7 cut(s) 390, 526, 637, 689, 776, 1075, 1213
Alw21I GWGCWC 1 cut(s) 273
Alw26I GTCTC 7 cut(s) 438, 459, 545, 596, 646, 1243, 1318
Alw44I GTGCAC 1 cut(s) 269
AlwI GGATC 3 cut(s) 144, 613, 1074
AlwNI CAGNNNCTG 1 cut(s) 719
Ama87I CYCGRG 2 cut(s) 557, 630
AoxI GGCC 3 cut(s) 210, 405, 1037
ApaLI GTGCAC 1 cut(s) 269
ApeKI GCWGC 5 cut(s) 258, 351, 354, 381, 686
ApoI RAATTY 2 cut(s) 863, 1154
ArsI GACNNNNNNTTYG 4 cut(s) 186, 218, 695, 727
AspLEI GCGC 1 cut(s) 1306
AspS9I GGNCC 1 cut(s) 1037
AsuC2I CCSGG 2 cut(s) 16, 175
AsuHPI GGTGA 1 cut(s) 355
AvaI CYCGRG 2 cut(s) 557, 630
BaeGI GKGCMC 1 cut(s) 273
BalI TGGCCA 1 cut(s) 212
BanII GRGCYC 1 cut(s) 1354
BarI GAAGNNNNNNTAC 2 cut(s) 735, 767
BbsI GAAGAC 1 cut(s) 445
Bbv12I GWGCWC 1 cut(s) 273
BbvCI CCTCAGC 1 cut(s) 402
BbvI GCAGC 5 cut(s) 270, 363, 366, 393, 698
BccI CCATC 6 cut(s) 224, 290, 529, 736, 763, 833
BceAI ACGGC 1 cut(s) 841
BciT130I CCWGG 3 cut(s) 222, 1041, 1329
BcnI CCSGG 2 cut(s) 16, 175
BcoDI GTCTC 7 cut(s) 438, 459, 545, 596, 646, 1243, 1318
BfmI CTRYAG 1 cut(s) 681
BfrI CTTAAG 1 cut(s) 962
BisI GCNGC 9 cut(s) 99, 131, 226, 256, 259, 352, 355, 382, 687
BlpI GCTNAGC 1 cut(s) 377
BlsI GCNGC 9 cut(s) 100, 132, 227, 257, 260, 353, 356, 383, 688
Bme1390I CCNGG 5 cut(s) 16, 175, 222, 1041, 1329
BmeT110I CYCGRG 2 cut(s) 557, 630
BmgT120I GGNCC 1 cut(s) 1037
BmrFI CCNGG 5 cut(s) 16, 175, 222, 1041, 1329
BpiI GAAGAC 1 cut(s) 445
BplI GAGNNNNNCTC 2 cut(s) 626, 658
Bpu10I CCTNAGC 1 cut(s) 402
Bpu1102I GCTNAGC 1 cut(s) 377
BpuMI CCSGG 2 cut(s) 16, 175
BsaBI GATNNNNATC 1 cut(s) 843
BsaI GGTCTC 2 cut(s) 596, 1318
BsaJI CCNNGG 1 cut(s) 114
BsaXI ACNNNNNCTCC 2 cut(s) 297, 327
Bsc4I CCNNNNNNNGG 4 cut(s) 15, 1206, 1295, 1296
Bse8I GATNNNNATC 1 cut(s) 843
BseBI CCWGG 3 cut(s) 222, 1041, 1329
BseDI CCNNGG 1 cut(s) 114
BseGI GGATG 1 cut(s) 838
BseJI GATNNNNATC 1 cut(s) 843
BseLI CCNNNNNNNGG 4 cut(s) 15, 1206, 1295, 1296
BseMII CTCAG 4 cut(s) 393, 657, 861, 1227
BseRI GAGGAG 2 cut(s) 750, 753
BseSI GKGCMC 1 cut(s) 273
BseXI GCAGC 5 cut(s) 270, 363, 366, 393, 698
Bsh1236I CGCG 1 cut(s) 1306
Bsh1285I CGRYCG 1 cut(s) 90
BshFI GGCC 3 cut(s) 212, 407, 1039
BsiEI CGRYCG 1 cut(s) 90
BsiHKAI GWGCWC 1 cut(s) 273
BsiHKCI CYCGRG 2 cut(s) 557, 630
BsiSI CCGG 2 cut(s) 15, 175
BslI CCNNNNNNNGG 4 cut(s) 15, 1206, 1295, 1296
BsmAI GTCTC 7 cut(s) 438, 459, 545, 596, 646, 1243, 1318
BsnI GGCC 3 cut(s) 212, 407, 1039
Bso31I GGTCTC 2 cut(s) 596, 1318
BsoBI CYCGRG 2 cut(s) 557, 630
Bsp1286I GDGCHC 2 cut(s) 273, 1354
Bsp143I GATC 6 cut(s) 87, 136, 605, 1066, 1092, 1282
Bsp1720I GCTNAGC 1 cut(s) 377
BspACI CCGC 9 cut(s) 98, 130, 133, 151, 225, 255, 321, 372, 581
BspANI GGCC 3 cut(s) 212, 407, 1039
BspCNI CTCAG 4 cut(s) 394, 656, 862, 1228
BspFNI CGCG 1 cut(s) 1306
BspPI GGATC 3 cut(s) 144, 613, 1074
BspTI CTTAAG 1 cut(s) 962
BspTNI GGTCTC 2 cut(s) 596, 1318
BsrBI CCGCTC 1 cut(s) 151
BssECI CCNNGG 1 cut(s) 114
BssMI GATC 6 cut(s) 87, 136, 605, 1066, 1092, 1282
Bst2UI CCWGG 3 cut(s) 222, 1041, 1329
Bst4CI ACNGT 5 cut(s) 217, 502, 878, 1082, 1261
BstAFI CTTAAG 1 cut(s) 962
BstAPI GCANNNNNTGC 1 cut(s) 692
BstC8I GCNNGC 3 cut(s) 374, 697, 1302
BstDEI CTNAG 5 cut(s) 377, 402, 643, 870, 1236
BstDSI CCRYGG 1 cut(s) 114
BstF5I GGATG 1 cut(s) 838
BstFNI CGCG 1 cut(s) 1306
BstHHI GCGC 1 cut(s) 1306
BstKTI GATC 6 cut(s) 90, 139, 608, 1069, 1095, 1285
BstMAI GTCTC 7 cut(s) 438, 459, 545, 596, 646, 1243, 1318
BstMBI GATC 6 cut(s) 87, 136, 605, 1066, 1092, 1282
BstMCI CGRYCG 1 cut(s) 90
BstMWI GCNNNNNNNGC 8 cut(s) 378, 387, 686, 692, 782, 1015, 1219, 1241
BstNI CCWGG 3 cut(s) 222, 1041, 1329
BstNSI RCATGY 1 cut(s) 472
BstSCI CCNGG 5 cut(s) 14, 173, 220, 1039, 1327
BstSFI CTRYAG 1 cut(s) 681
BstSLI GKGCMC 1 cut(s) 273
BstUI CGCG 1 cut(s) 1306
BstV1I GCAGC 5 cut(s) 270, 363, 366, 393, 698
BstV2I GAAGAC 1 cut(s) 445
BsuRI GGCC 3 cut(s) 212, 407, 1039
BtgI CCRYGG 1 cut(s) 114
BtgZI GCGATG 1 cut(s) 243
BtsCI GGATG 1 cut(s) 838
BtsIMutI CAGTG 3 cut(s) 20, 867, 1087
Cac8I GCNNGC 3 cut(s) 374, 697, 1302
CaiI CAGNNNCTG 1 cut(s) 719
CfoI GCGC 1 cut(s) 1306
Cfr13I GGNCC 1 cut(s) 1037
Csp6I GTAC 2 cut(s) 874, 1262
CviAII CATG 7 cut(s) 83, 469, 602, 658, 829, 1280, 1355
CviQI GTAC 2 cut(s) 874, 1262
DdeI CTNAG 5 cut(s) 377, 402, 643, 870, 1236
DpnI GATC 6 cut(s) 89, 138, 607, 1068, 1094, 1284
DpnII GATC 6 cut(s) 87, 136, 605, 1066, 1092, 1282
EaeI YGGCCR 1 cut(s) 210
Eco147I AGGCCT 1 cut(s) 407
Eco24I GRGCYC 1 cut(s) 1354
Eco31I GGTCTC 2 cut(s) 596, 1318
Eco57I CTGAAG 2 cut(s) 439, 512
Eco88I CYCGRG 2 cut(s) 557, 630
EcoRI GAATTC 1 cut(s) 863
EcoRII CCWGG 3 cut(s) 220, 1039, 1327
EcoT38I GRGCYC 1 cut(s) 1354
FaeI CATG 7 cut(s) 86, 472, 605, 661, 832, 1283, 1358
FalI AAGNNNNNCTT 2 cut(s) 765, 797
FatI CATG 7 cut(s) 82, 468, 601, 657, 828, 1279, 1354
FauI CCCGC 2 cut(s) 126, 365
Fnu4HI GCNGC 9 cut(s) 99, 131, 226, 256, 259, 352, 355, 382, 687
FokI GGATG 1 cut(s) 845
FriOI GRGCYC 1 cut(s) 1354
Fsp4HI GCNGC 9 cut(s) 99, 131, 226, 256, 259, 352, 355, 382, 687
GlaI GCGC 1 cut(s) 1305
GluI GCNGC 9 cut(s) 99, 131, 226, 256, 259, 352, 355, 382, 687
HaeIII GGCC 3 cut(s) 212, 407, 1039
HapII CCGG 2 cut(s) 15, 175
HhaI GCGC 1 cut(s) 1306
Hin1II CATG 7 cut(s) 86, 472, 605, 661, 832, 1283, 1358
Hin6I GCGC 1 cut(s) 1304
HinP1I GCGC 1 cut(s) 1304
HindIII AAGCTT 2 cut(s) 524, 774
HinfI GANTC 7 cut(s) 431, 491, 512, 553, 616, 716, 1312
HpaII CCGG 2 cut(s) 15, 175
HphI GGTGA 1 cut(s) 355
Hpy166II GTNNAC 1 cut(s) 271
Hpy188I TCNGA 8 cut(s) 48, 61, 106, 141, 496, 543, 715, 1168
Hpy188III TCNNGA 5 cut(s) 457, 476, 557, 632, 767
Hpy8I GTNNAC 1 cut(s) 271
Hpy99I CGWCG 2 cut(s) 251, 266
HpyAV CCTTC 3 cut(s) 230, 245, 740
HpyCH4III ACNGT 5 cut(s) 217, 502, 878, 1082, 1261
HpyCH4V TGCA 4 cut(s) 53, 271, 1178, 1279
HpyF10VI GCNNNNNNNGC 8 cut(s) 378, 387, 686, 692, 782, 1015, 1219, 1241
HpyF3I CTNAG 5 cut(s) 377, 402, 643, 870, 1236
Hsp92II CATG 7 cut(s) 86, 472, 605, 661, 832, 1283, 1358
HspAI GCGC 1 cut(s) 1304
Kzo9I GATC 6 cut(s) 87, 136, 605, 1066, 1092, 1282
LmnI GCTCC 1 cut(s) 1218
Lsp1109I GCAGC 5 cut(s) 270, 363, 366, 393, 698
MaeIII GTNAC 1 cut(s) 18
MalI GATC 6 cut(s) 89, 138, 607, 1068, 1094, 1284
MbiI CCGCTC 1 cut(s) 151
MboI GATC 6 cut(s) 87, 136, 605, 1066, 1092, 1282
MboII GAAGA 4 cut(s) 445, 755, 900, 1000
MhlI GDGCHC 2 cut(s) 273, 1354
MlsI TGGCCA 1 cut(s) 212
MluCI AATT 5 cut(s) 25, 206, 570, 863, 1154
MluNI TGGCCA 1 cut(s) 212
MlyI GAGTC 4 cut(s) 440, 500, 506, 547
MmeI TCCRAC 1 cut(s) 629
Mox20I TGGCCA 1 cut(s) 212
MscI TGGCCA 1 cut(s) 212
MseI TTAA 3 cut(s) 723, 921, 963
Msp20I TGGCCA 1 cut(s) 212
MspCI CTTAAG 1 cut(s) 962
MspI CCGG 2 cut(s) 15, 175
MspR9I CCNGG 5 cut(s) 16, 175, 222, 1041, 1329
MvaI CCWGG 3 cut(s) 222, 1041, 1329
MvnI CGCG 1 cut(s) 1306
MwoI GCNNNNNNNGC 8 cut(s) 378, 387, 686, 692, 782, 1015, 1219, 1241
NciI CCSGG 2 cut(s) 16, 175
NdeII GATC 6 cut(s) 87, 136, 605, 1066, 1092, 1282
NlaIII CATG 7 cut(s) 86, 472, 605, 661, 832, 1283, 1358
NmuCI GTSAC 1 cut(s) 18
NspI RCATGY 1 cut(s) 472
PaeR7I CTCGAG 2 cut(s) 557, 630
PceI AGGCCT 1 cut(s) 407
PciI ACATGT 1 cut(s) 468
PfeI GAWTC 3 cut(s) 616, 716, 1312
PkrI GCNGC 9 cut(s) 100, 132, 227, 257, 260, 353, 356, 383, 688
Ple19I CGATCG 1 cut(s) 90
PleI GAGTC 4 cut(s) 439, 499, 506, 547
PpsI GAGTC 4 cut(s) 439, 499, 506, 547
PscI ACATGT 1 cut(s) 468
Psp6I CCWGG 3 cut(s) 220, 1039, 1327
PspGI CCWGG 3 cut(s) 220, 1039, 1327
PspPI GGNCC 1 cut(s) 1037
PstNI CAGNNNCTG 1 cut(s) 719
PvuI CGATCG 1 cut(s) 90
RsaI GTAC 2 cut(s) 875, 1263
RsaNI GTAC 2 cut(s) 874, 1262
SaqAI TTAA 3 cut(s) 723, 921, 963
SatI GCNGC 9 cut(s) 99, 131, 226, 256, 259, 352, 355, 382, 687
Sau3AI GATC 6 cut(s) 87, 136, 605, 1066, 1092, 1282
Sau96I GGNCC 1 cut(s) 1037
SchI GAGTC 4 cut(s) 440, 500, 506, 547
ScrFI CCNGG 5 cut(s) 16, 175, 222, 1041, 1329
SduI GDGCHC 2 cut(s) 273, 1354
SfcI CTRYAG 1 cut(s) 681
Sfr274I CTCGAG 2 cut(s) 557, 630
SlaI CTCGAG 2 cut(s) 557, 630
SmlI CTYRAG 3 cut(s) 557, 630, 962
SmoI CTYRAG 3 cut(s) 557, 630, 962
Sse9I AATT 5 cut(s) 25, 206, 570, 863, 1154
SseBI AGGCCT 1 cut(s) 407
SsiI CCGC 9 cut(s) 98, 130, 133, 151, 225, 255, 321, 372, 581
SspI AATATT 1 cut(s) 938
StuI AGGCCT 1 cut(s) 407
StyD4I CCNGG 5 cut(s) 14, 173, 220, 1039, 1327
TaaI ACNGT 5 cut(s) 217, 502, 878, 1082, 1261
TaqI TCGA 8 cut(s) 78, 204, 315, 558, 631, 850, 1095, 1194
TasI AATT 5 cut(s) 25, 206, 570, 863, 1154
TauI GCSGC 4 cut(s) 101, 133, 228, 258
TfiI GAWTC 3 cut(s) 616, 716, 1312
Tru1I TTAA 3 cut(s) 723, 921, 963
Tru9I TTAA 3 cut(s) 723, 921, 963
TscAI CASTG 3 cut(s) 27, 874, 1087
TseFI GTSAC 1 cut(s) 18
TseI GCWGC 5 cut(s) 258, 351, 354, 381, 686
Tsp45I GTSAC 1 cut(s) 18
TspDTI ATGAA 5 cut(s) 681, 756, 786, 993, 1185
TspGWI ACGGA 1 cut(s) 131
TspRI CASTG 3 cut(s) 27, 874, 1087
Vha464I CTTAAG 1 cut(s) 962
VneI GTGCAC 1 cut(s) 269
XapI RAATTY 2 cut(s) 863, 1154
XceI RCATGY 1 cut(s) 472
XcmI CCANNNNNNNNNTGG 1 cut(s) 1060
XhoI CTCGAG 2 cut(s) 557, 630
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.