Rw2G039210

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0010201
Physical Location & Seq
Forward (+)
64176693 .. 64178275
1583 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G039210.1

Sequence Viewer

Length: 1410 bp
ATGGCGACCACGGCCAAGTCTGGGTCTTCACTGAATTACTGGAGGAATTATTTCCGGACGGCGAATTCGGACATTTTCGGCATCATTGACCATGCGATCATGGTTGCCGCTGTGGATTGTCCCAAGGAGTTTAGGTTGCGGAGGGATCGAGTTGCTGAGCTCTTGTTTTCCTGCAGAATGAGCAGGTGTTCGGGTTGCGAAAAGGTGGAGTTGGTGGTGCCTGGTGCTCATGCTCATGATGATCACTATGAGGTGGAGCATGATGATGATGATGATGATGACCGAGGAGGTGATAGAGATTTTGAGGCCGGGGCTAGTAAAGAGAGCAAGGTCAATAGCAGCAGAGATGATCAGATTGGTGAAATGAGCATGAAGAATGATCAGGATAGCAATTACAGCTATGGATTGGCTGAGGCTTTGACCGATGAGATTGAACAAGAGTCTCAGGTTTTCGGGGAGGTTGTGAGGATCAAAGAGATCTTCCAGAATAGTGAACATGAGTCTGATGCTGTATTGTTTGAGTCGTTGAGGAAGCTTGAGTTGATGGCTCTGTCTGTGGAGACTCTCAAGGCAACTGAGATTGGGAAGGCTGTTAACCGGCTAAGAAAGCATGGATCAATGAAGATTCGTAATCTTGTTCGAACACTAATTGATGGGTGGAAAGTCATGGTAGATGAATGGGTCAATGCTACTACAGCTATTGCTTCTGGGGGAACAGAAGTTATTTCGGATTCTGTTGATCCATCAATTGATGATGACTTTGAAGAAGGACTTCCCACTCCTCCATTGGATGAAGGAGCTTTCTTTGCCACTCAAACCACCTCAATGGAACTCTCTGAGTTCTTTGATGGTATGGAAGATTATGGGAATCCTCGAAACAGTGGGCAATATACCAGGAATCGTGAAAATGGAAGAAAACCATCACTGGAGAAACAAAATGTTGTGAAGCAGGAACAACAGTATGTTAGTGATCAGGGAAATGTGCTTCCCAAGCAAATAAAAAGTGAACCAGTGACGGTGAAACAAGAAGTTGATGTGAAGCCAAATAGGCCCTCAAACACCAACTCTGGCCCTCGGAGACCTCCCAAAGTAAGTATGGAGCGAAAGGGTGTCAGTAGTGTAACAAATATTGAGCAAAGAACAGATAAGGTTTCCATTCAGAAGAGGCAACTCACTGGTCAGCAGCAAGATAAATACAAGTGTTCAGATGAAGTTGCAGTGCAAGTGAAGCTTGAAGCCACAAAAAGGAAACTCCAGGAGAGTTATCAACAAGCTGAGAATGCCAAGAAGCAACGGACAGTACAGGTAATGGAATTGCATGATCTACCTAAGCAGGGGCTTGGCCATAGGAACCCACATTTAAAACCTGGAAACCACAATCGGCATAGGGCACATGGGCGGAGATTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Med26

Protein Analysis

469

Amino Acids

53.01

Weight (kDa)

5.74

Isoelectric Point (pI)

37.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med26 PF08711 175 - 224 2.5e-13 TFIIS helical bundle-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 174
Acc36I ACCTGC 1 cut(s) 174
AccB1I GGYRCC 1 cut(s) 217
AccIII TCCGGA 1 cut(s) 54
AciI CCGC 3 cut(s) 108, 139, 1399
AclWI GGATC 4 cut(s) 153, 476, 622, 734
AcoI YGGCCR 2 cut(s) 12, 1342
AcsI RAATTY 1 cut(s) 64
AfaI GTAC 1 cut(s) 1302
AfiI CCNNNNNNNGG 3 cut(s) 21, 1245, 1334
AgsI TTSAA 3 cut(s) 434, 764, 1235
AjnI CCWGG 4 cut(s) 220, 893, 1254, 1366
AluBI AGCT 7 cut(s) 160, 399, 535, 698, 800, 1231, 1274
AluI AGCT 7 cut(s) 160, 399, 535, 698, 800, 1231, 1274
Alw21I GWGCWC 2 cut(s) 162, 229
Alw26I GTCTC 3 cut(s) 447, 554, 1072
AlwI GGATC 4 cut(s) 153, 476, 622, 734
Aor13HI TCCGGA 1 cut(s) 54
AoxI GGCC 5 cut(s) 12, 306, 1049, 1069, 1342
ApeKI GCWGC 2 cut(s) 339, 1183
ApoI RAATTY 1 cut(s) 64
ArsI GACNNNNNNTTYG 2 cut(s) 49, 81
Asp700I GAANNNNTTC 2 cut(s) 50, 771
AspS9I GGNCC 2 cut(s) 1050, 1070
AsuC2I CCSGG 1 cut(s) 310
AsuHPI GGTGA 3 cut(s) 302, 371, 1030
AsuII TTCGAA 1 cut(s) 640
BaeGI GKGCMC 1 cut(s) 1393
BalI TGGCCA 1 cut(s) 1344
BanI GGYRCC 1 cut(s) 217
BanII GRGCYC 1 cut(s) 162
BbsI GAAGAC 1 cut(s) 18
Bbv12I GWGCWC 2 cut(s) 162, 229
BbvCI CCTCAGC 1 cut(s) 411
BbvI GCAGC 2 cut(s) 351, 1195
BccI CCATC 5 cut(s) 538, 647, 751, 842, 928
BceAI ACGGC 2 cut(s) 27, 75
BciT130I CCWGG 4 cut(s) 222, 895, 1256, 1368
BclI TGATCA 4 cut(s) 241, 349, 379, 970
BcnI CCSGG 1 cut(s) 310
BcoDI GTCTC 3 cut(s) 447, 554, 1072
BfaI CTAG 1 cut(s) 315
BfmI CTRYAG 2 cut(s) 172, 693
BfuAI ACCTGC 1 cut(s) 174
BglI GCCNNNNNGGC 1 cut(s) 1048
BglII AGATCT 1 cut(s) 477
BisI GCNGC 3 cut(s) 108, 340, 1184
BlpI GCTNAGC 1 cut(s) 156
BlsI GCNGC 3 cut(s) 109, 341, 1185
Bme1390I CCNGG 5 cut(s) 222, 310, 895, 1256, 1368
BmgT120I GGNCC 2 cut(s) 1050, 1070
BmiI GGNNCC 2 cut(s) 219, 1352
BmrFI CCNGG 5 cut(s) 222, 310, 895, 1256, 1368
BmsI GCATC 2 cut(s) 90, 496
BpiI GAAGAC 1 cut(s) 18
BpmI CTGGAG 3 cut(s) 61, 947, 1238
Bpu10I CCTNAGC 2 cut(s) 411, 1329
Bpu1102I GCTNAGC 1 cut(s) 156
Bpu14I TTCGAA 1 cut(s) 640
BpuEI CTTGAG 2 cut(s) 551, 557
BpuMI CCSGG 1 cut(s) 310
BsaI GGTCTC 1 cut(s) 1072
BsaJI CCNNGG 5 cut(s) 9, 123, 283, 309, 1073
BsaWI WCCGGW 1 cut(s) 54
BsaXI ACNNNNNCTCC 2 cut(s) 119, 149
Bsc4I CCNNNNNNNGG 3 cut(s) 21, 1245, 1334
Bse118I RCCGGY 1 cut(s) 597
Bse1I ACTGG 4 cut(s) 44, 930, 1010, 1180
BseAI TCCGGA 1 cut(s) 54
BseBI CCWGG 4 cut(s) 222, 895, 1256, 1368
BseDI CCNNGG 5 cut(s) 9, 123, 283, 309, 1073
BseGI GGATG 1 cut(s) 796
BseLI CCNNNNNNNGG 3 cut(s) 21, 1245, 1334
BseMII CTCAG 6 cut(s) 147, 402, 458, 567, 828, 1266
BseNI ACTGG 4 cut(s) 44, 930, 1010, 1180
BseRI GAGGAG 2 cut(s) 300, 771
BseSI GKGCMC 1 cut(s) 1393
BseXI GCAGC 2 cut(s) 351, 1195
BshFI GGCC 5 cut(s) 14, 308, 1051, 1071, 1344
BshNI GGYRCC 1 cut(s) 217
BsiHKAI GWGCWC 2 cut(s) 162, 229
BsiSI CCGG 3 cut(s) 55, 309, 598
BslFI GGGAC 1 cut(s) 105
BslI CCNNNNNNNGG 3 cut(s) 21, 1245, 1334
BsmAI GTCTC 3 cut(s) 447, 554, 1072
BsmFI GGGAC 1 cut(s) 105
BsmI GAATGC 1 cut(s) 1285
BsnI GGCC 5 cut(s) 14, 308, 1051, 1071, 1344
Bso31I GGTCTC 1 cut(s) 1072
Bsp119I TTCGAA 1 cut(s) 640
Bsp1286I GDGCHC 3 cut(s) 162, 229, 1393
Bsp13I TCCGGA 1 cut(s) 54
Bsp1720I GCTNAGC 1 cut(s) 156
BspACI CCGC 3 cut(s) 108, 139, 1399
BspANI GGCC 5 cut(s) 14, 308, 1051, 1071, 1344
BspCNI CTCAG 6 cut(s) 148, 403, 457, 568, 829, 1267
BspEI TCCGGA 1 cut(s) 54
BspHI TCATGA 1 cut(s) 235
BspLI GGNNCC 2 cut(s) 219, 1352
BspMAI CTGCAG 1 cut(s) 176
BspMI ACCTGC 1 cut(s) 174
BspPI GGATC 4 cut(s) 153, 476, 622, 734
BspT104I TTCGAA 1 cut(s) 640
BspT107I GGYRCC 1 cut(s) 217
BspTNI GGTCTC 1 cut(s) 1072
BsrFI RCCGGY 1 cut(s) 597
BsrI ACTGG 4 cut(s) 44, 930, 1010, 1180
BssAI RCCGGY 1 cut(s) 597
BssECI CCNNGG 5 cut(s) 9, 123, 283, 309, 1073
BssT1I CCWWGG 1 cut(s) 123
Bst2UI CCWGG 4 cut(s) 222, 895, 1256, 1368
Bst4CI ACNGT 4 cut(s) 881, 960, 1018, 1300
Bst6I CTCTTC 1 cut(s) 1157
BstBI TTCGAA 1 cut(s) 640
BstDEI CTNAG 8 cut(s) 156, 411, 444, 576, 602, 837, 1275, 1329
BstDSI CCRYGG 1 cut(s) 9
BstF5I GGATG 1 cut(s) 796
BstMAI GTCTC 3 cut(s) 447, 554, 1072
BstNI CCWGG 4 cut(s) 222, 895, 1256, 1368
BstSCI CCNGG 5 cut(s) 220, 308, 893, 1254, 1366
BstSFI CTRYAG 2 cut(s) 172, 693
BstSLI GKGCMC 1 cut(s) 1393
BstV1I GCAGC 2 cut(s) 351, 1195
BstV2I GAAGAC 1 cut(s) 18
BstX2I RGATCY 1 cut(s) 477
BstXI CCANNNNNNTGG 1 cut(s) 826
BstYI RGATCY 1 cut(s) 477
BsuRI GGCC 5 cut(s) 14, 308, 1051, 1071, 1344
BtgI CCRYGG 1 cut(s) 9
BtsCI GGATG 1 cut(s) 796
BtsI GCAGTG 1 cut(s) 1224
BtsIMutI CAGTG 6 cut(s) 29, 886, 923, 1017, 1173, 1224
BveI ACCTGC 1 cut(s) 174
CciI TCATGA 1 cut(s) 235
Cfr10I RCCGGY 1 cut(s) 597
Cfr13I GGNCC 2 cut(s) 1050, 1070
Csp6I GTAC 1 cut(s) 1301
CviQI GTAC 1 cut(s) 1301
DdeI CTNAG 8 cut(s) 156, 411, 444, 576, 602, 837, 1275, 1329
DraI TTTAAA 1 cut(s) 1362
EaeI YGGCCR 2 cut(s) 12, 1342
Eam1104I CTCTTC 1 cut(s) 1157
EarI CTCTTC 1 cut(s) 1157
Ecl136II GAGCTC 1 cut(s) 160
Eco130I CCWWGG 1 cut(s) 123
Eco24I GRGCYC 1 cut(s) 162
Eco31I GGTCTC 1 cut(s) 1072
Eco53kI GAGCTC 1 cut(s) 160
EcoICRI GAGCTC 1 cut(s) 160
EcoO109I RGGNCCY 1 cut(s) 1050
EcoRI GAATTC 1 cut(s) 64
EcoRII CCWGG 4 cut(s) 220, 893, 1254, 1366
EcoT14I CCWWGG 1 cut(s) 123
EcoT38I GRGCYC 1 cut(s) 162
ErhI CCWWGG 1 cut(s) 123
FalI AAGNNNNNCTT 4 cut(s) 756, 788, 1215, 1247
FaqI GGGAC 1 cut(s) 105
FbaI TGATCA 4 cut(s) 241, 349, 379, 970
Fnu4HI GCNGC 3 cut(s) 108, 340, 1184
FokI GGATG 1 cut(s) 803
FriOI GRGCYC 1 cut(s) 162
Fsp4HI GCNGC 3 cut(s) 108, 340, 1184
FspBI CTAG 1 cut(s) 315
GluI GCNGC 3 cut(s) 108, 340, 1184
GsuI CTGGAG 3 cut(s) 61, 947, 1238
HaeIII GGCC 5 cut(s) 14, 308, 1051, 1071, 1344
HapII CCGG 3 cut(s) 55, 309, 598
HincII GTYRAC 1 cut(s) 595
HindII GTYRAC 1 cut(s) 595
HindIII AAGCTT 2 cut(s) 533, 1229
HinfI GANTC 8 cut(s) 440, 500, 521, 562, 625, 731, 868, 898
HpaI GTTAAC 1 cut(s) 595
HpaII CCGG 3 cut(s) 55, 309, 598
HphI GGTGA 3 cut(s) 302, 371, 1030
Hpy166II GTNNAC 3 cut(s) 494, 595, 1007
Hpy188I TCNGA 8 cut(s) 70, 354, 505, 730, 838, 1077, 1161, 1207
Hpy188III TCNNGA 5 cut(s) 55, 236, 383, 484, 902
Hpy8I GTNNAC 3 cut(s) 494, 595, 1007
HpyAV CCTTC 3 cut(s) 580, 761, 788
HpyCH4III ACNGT 4 cut(s) 881, 960, 1018, 1300
HpyCH4V TGCA 4 cut(s) 174, 1217, 1222, 1318
HpyF3I CTNAG 8 cut(s) 156, 411, 444, 576, 602, 837, 1275, 1329
Kpn2I TCCGGA 1 cut(s) 54
Ksp22I TGATCA 4 cut(s) 241, 349, 379, 970
KspAI GTTAAC 1 cut(s) 595
LmnI GCTCC 3 cut(s) 256, 797, 1099
Lsp1109I GCAGC 2 cut(s) 351, 1195
LweI GCATC 2 cut(s) 90, 496
MaeI CTAG 1 cut(s) 315
MaeIII GTNAC 2 cut(s) 1012, 1120
MboII GAAGA 8 cut(s) 18, 385, 472, 634, 776, 869, 924, 1174
MfeI CAATTG 1 cut(s) 747
MflI RGATCY 1 cut(s) 477
MhlI GDGCHC 3 cut(s) 162, 229, 1393
MlsI TGGCCA 1 cut(s) 1344
MluCI AATT 7 cut(s) 34, 46, 64, 391, 648, 747, 1313
MluNI TGGCCA 1 cut(s) 1344
MlyI GAGTC 4 cut(s) 449, 509, 530, 556
Mox20I TGGCCA 1 cut(s) 1344
MroI TCCGGA 1 cut(s) 54
MroXI GAANNNNTTC 2 cut(s) 50, 771
MscI TGGCCA 1 cut(s) 1344
MseI TTAA 2 cut(s) 594, 1361
MslI CAYNNNNRTG 3 cut(s) 234, 264, 824
Msp20I TGGCCA 1 cut(s) 1344
MspA1I CMGCKG 1 cut(s) 110
MspI CCGG 3 cut(s) 55, 309, 598
MspR9I CCNGG 5 cut(s) 222, 310, 895, 1256, 1368
MunI CAATTG 1 cut(s) 747
Mva1269I GAATGC 1 cut(s) 1285
MvaI CCWGG 4 cut(s) 222, 895, 1256, 1368
NciI CCSGG 1 cut(s) 310
NlaIV GGNNCC 2 cut(s) 219, 1352
NmuCI GTSAC 1 cut(s) 1012
NspV TTCGAA 1 cut(s) 640
PagI TCATGA 1 cut(s) 235
PaqCI CACCTGC 1 cut(s) 174
PctI GAATGC 1 cut(s) 1285
PdmI GAANNNNTTC 2 cut(s) 50, 771
PfeI GAWTC 4 cut(s) 625, 731, 868, 898
PfoI TCCNGGA 1 cut(s) 1254
PkrI GCNGC 3 cut(s) 109, 341, 1185
PleI GAGTC 4 cut(s) 448, 508, 529, 556
PpsI GAGTC 4 cut(s) 448, 508, 529, 556
Psp124BI GAGCTC 1 cut(s) 162
Psp6I CCWGG 4 cut(s) 220, 893, 1254, 1366
PspGI CCWGG 4 cut(s) 220, 893, 1254, 1366
PspN4I GGNNCC 2 cut(s) 219, 1352
PspPI GGNCC 2 cut(s) 1050, 1070
PstI CTGCAG 1 cut(s) 176
PsuI RGATCY 1 cut(s) 477
RsaI GTAC 1 cut(s) 1302
RsaNI GTAC 1 cut(s) 1301
RseI CAYNNNNRTG 3 cut(s) 234, 264, 824
SacI GAGCTC 1 cut(s) 162
SaqAI TTAA 2 cut(s) 594, 1361
SatI GCNGC 3 cut(s) 108, 340, 1184
Sau96I GGNCC 2 cut(s) 1050, 1070
SchI GAGTC 4 cut(s) 449, 509, 530, 556
ScrFI CCNGG 5 cut(s) 222, 310, 895, 1256, 1368
SduI GDGCHC 3 cut(s) 162, 229, 1393
SfaNI GCATC 2 cut(s) 90, 496
SfcI CTRYAG 2 cut(s) 172, 693
SfuI TTCGAA 1 cut(s) 640
SmiMI CAYNNNNRTG 3 cut(s) 234, 264, 824
SmlI CTYRAG 2 cut(s) 536, 566
SmoI CTYRAG 2 cut(s) 536, 566
Sse9I AATT 7 cut(s) 34, 46, 64, 391, 648, 747, 1313
SsiI CCGC 3 cut(s) 108, 139, 1399
SspI AATATT 1 cut(s) 1129
SspMI CTAG 1 cut(s) 315
SstI GAGCTC 1 cut(s) 162
StyD4I CCNGG 5 cut(s) 220, 308, 893, 1254, 1366
StyI CCWWGG 1 cut(s) 123
TaaI ACNGT 4 cut(s) 881, 960, 1018, 1300
TaqI TCGA 3 cut(s) 148, 640, 874
TaqII GACCGA 2 cut(s) 297, 437
TasI AATT 7 cut(s) 34, 46, 64, 391, 648, 747, 1313
TatI WGTACW 1 cut(s) 1300
TauI GCSGC 1 cut(s) 110
TfiI GAWTC 4 cut(s) 625, 731, 868, 898
Tru1I TTAA 2 cut(s) 594, 1361
Tru9I TTAA 2 cut(s) 594, 1361
TscAI CASTG 6 cut(s) 36, 886, 930, 1017, 1180, 1224
TseFI GTSAC 1 cut(s) 1012
TseI GCWGC 2 cut(s) 339, 1183
Tsp45I GTSAC 1 cut(s) 1012
TspDTI ATGAA 5 cut(s) 386, 635, 690, 807, 1224
TspGWI ACGGA 1 cut(s) 1309
TspRI CASTG 6 cut(s) 36, 886, 930, 1017, 1180, 1224
XapI RAATTY 1 cut(s) 64
XcmI CCANNNNNNNNNTGG 2 cut(s) 784, 1093
XmnI GAANNNNTTC 2 cut(s) 50, 771
XspI CTAG 1 cut(s) 315
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.