AT5G05140

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity). May play a role in transcription elongation (By similarity)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0010201
Physical Location & Seq
Forward (+)
1519705 .. 1523137
3433 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G05140.1

Sequence Viewer

Length: 1311 bp
ATGAAAGCATCGGGTTCATTAGATAGCTGGAGAGAATATTTCCGACGGCGAGGAGATTCCGATATATTCGGGATCATCGATCATGCGATCATGGTGGCTGCTACTGATTGTCCTAACAAATTCAAATCCAGAAGAGACAAAATCGCCGAGCTTTTATTCTCCTGTAGAGTGAATCGTTGCGTCGGATGCGACCATCTTGAATTGTCTGTTCCCGGAGACGACGAGGCTAACCGTGGAACCACCGGAAATGGTGGTGGTGGTACGGCGGTTGATGAAGATTATGAGGTTGCTGGTGGTAGTAAAGAGAGCAAAGCTAATAGTAGCAGAGGGGATAATAATCAGATTGTTAGCAATTATACATTTGATGAAGCTGAGGCTTTGAGTGATGAGATTGAAGAGTTTTCTGTGGTTTCTAAGGAGGTTGCTAGGATCAAAGAGATCTTGCTCAACAAAGAAGATGAGCCAAACTCGGTGTTACTCGATTCTTTAAGACATCTTAAGTTGATGTCTTTGAATGTGGATATTCTTAAGAGTACTGAGATTGGAAAGGCTGTTAATGGCCTCAGGAAACATTCTTCTGATAAGATTCGACAACTCGCTAAGACTCTTATCGCAGAATGGAAGGAGTTGGTGGATCAATGGGTGAATACCACAAAGGAAATCACTGGTGCTGAAGGTACACCAGAGTCTGCAAACCCGTCTGTCCTTGATGAAGAAGAGGCGTTTCCATCGCTTCCATATGATGTTGATATCTTTACACCTGAACCAAACGGATTTGAAATCTCACACTTCTTTGATTCCTTGGACTTTGATGGAAATCCTCGTAACTCTGAAGAACACAACACAAGCCGAGAGCATGAAAGAAGGCCACAAAATATCGCCAAGAGAAAACCTGAGGGAACACAAATGAGGATACAAGATGCTCCTTTTAGATCTATTAAGCCATCATCAGCTACTGATTTTGATGGGACTAGAAGACCTGTAAAGCAAAGTACAGAGCAAAGGATGAAGAACGAAACGGTATCTGTTCACAAGTCTGAAAAACCCATGATCCAAAGGAAACCGGTTGTTACAGAACAGAAGCGGAAAGCTCCTGGACCTCAACAAGAAAAACTTAAAGGTCTTGATGCAGACGCAAAGTTTGAGTTTGCAAAGAGGAAACTTCAAGAAAGCTACCAGCATCACGAGAATGCCAAGAAGCAGCGAACGATACAAGTACTTGAGATGATCCCAAAGCAAGGTAGTGCTCAGAAACCGCAACTCAAGAGACCTGGAATGAGCAACAGGAATTGGGCTAATGGGAGAAAATAG

Protein Analysis

436

Amino Acids

49.13

Weight (kDa)

6.6

Isoelectric Point (pI)

49.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med26 PF08711 162 - 211 3.2e-16 TFIIS helical bundle-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 266, 1084, 1256
AclWI GGATC 5 cut(s) 80, 437, 642, 1045, 1222
AcsI RAATTY 1 cut(s) 119
AcuI CTGAAG 2 cut(s) 693, 852
AfaI GTAC 5 cut(s) 262, 535, 679, 994, 1218
AfiI CCNNNNNNNGG 1 cut(s) 1238
AflII CTTAAG 2 cut(s) 497, 527
AgeI ACCGGT 1 cut(s) 1063
AgsI TTSAA 6 cut(s) 124, 200, 395, 514, 779, 1166
AjnI CCWGG 2 cut(s) 1093, 1270
AluBI AGCT 7 cut(s) 27, 151, 314, 371, 953, 1091, 1173
AluI AGCT 7 cut(s) 27, 151, 314, 371, 953, 1091, 1173
Alw21I GWGCWC 1 cut(s) 1249
Alw26I GTCTC 3 cut(s) 129, 210, 1261
AlwI GGATC 5 cut(s) 80, 437, 642, 1045, 1222
AlwNI CAGNNNCTG 2 cut(s) 689, 956
AoxI GGCC 2 cut(s) 559, 866
ApeKI GCWGC 2 cut(s) 98, 1201
ApoI RAATTY 1 cut(s) 119
ArsI GACNNNNNNTTYG 2 cut(s) 1124, 1156
AsiGI ACCGGT 1 cut(s) 1063
AspS9I GGNCC 1 cut(s) 1097
AsuC2I CCSGG 1 cut(s) 213
AsuHPI GGTGA 1 cut(s) 655
AvaII GGWCC 1 cut(s) 1097
AxyI CCTNAGG 2 cut(s) 563, 894
BaeI ACNNNNGTAYC 2 cut(s) 252, 285
BauI CACGAG 1 cut(s) 1184
BbsI GAAGAC 1 cut(s) 982
Bbv12I GWGCWC 1 cut(s) 1249
BbvCI CCTCAGC 1 cut(s) 372
BbvI GCAGC 2 cut(s) 85, 1213
BccI CCATC 5 cut(s) 201, 736, 806, 952, 959
BceAI ACGGC 2 cut(s) 62, 279
BciT130I CCWGG 2 cut(s) 1095, 1272
BciVI GTATCC 1 cut(s) 906
BcnI CCSGG 1 cut(s) 213
BcoDI GTCTC 3 cut(s) 129, 210, 1261
BfaI CTAG 2 cut(s) 426, 972
BfmI CTRYAG 1 cut(s) 163
BfrI CTTAAG 2 cut(s) 497, 527
BfuI GTATCC 1 cut(s) 906
BglII AGATCT 2 cut(s) 438, 932
BisI GCNGC 2 cut(s) 99, 1202
BlsI GCNGC 2 cut(s) 100, 1203
BmcAI AGTACT 2 cut(s) 535, 1218
Bme1390I CCNGG 3 cut(s) 213, 1095, 1272
Bme18I GGWCC 1 cut(s) 1097
BmgT120I GGNCC 1 cut(s) 1097
BmiI GGNNCC 1 cut(s) 238
BmrFI CCNGG 3 cut(s) 213, 1095, 1272
BmsI GCATC 5 cut(s) 17, 176, 910, 1117, 1189
BpiI GAAGAC 1 cut(s) 982
BplI GAGNNNNNCTC 2 cut(s) 452, 484
BpmI CTGGAG 1 cut(s) 49
Bpu10I CCTNAGC 1 cut(s) 372
BpuEI CTTGAG 2 cut(s) 1241, 1247
BpuMI CCSGG 1 cut(s) 213
Bsa29I ATCGAT 1 cut(s) 78
BsaBI GATNNNNATC 2 cut(s) 336, 816
BsaI GGTCTC 1 cut(s) 1261
BsaJI CCNNGG 2 cut(s) 232, 801
BsaWI WCCGGW 2 cut(s) 242, 1063
Bsc4I CCNNNNNNNGG 1 cut(s) 1238
Bse118I RCCGGY 1 cut(s) 1063
Bse1I ACTGG 1 cut(s) 670
Bse21I CCTNAGG 2 cut(s) 563, 894
Bse8I GATNNNNATC 2 cut(s) 336, 816
BseBI CCWGG 2 cut(s) 1095, 1272
BseCI ATCGAT 1 cut(s) 78
BseDI CCNNGG 2 cut(s) 232, 801
BseGI GGATG 2 cut(s) 191, 1011
BseJI GATNNNNATC 2 cut(s) 336, 816
BseLI CCNNNNNNNGG 1 cut(s) 1238
BseMII CTCAG 5 cut(s) 363, 528, 577, 885, 1262
BseNI ACTGG 1 cut(s) 670
BseRI GAGGAG 1 cut(s) 66
BseXI GCAGC 2 cut(s) 85, 1213
BshFI GGCC 2 cut(s) 561, 868
BshTI ACCGGT 1 cut(s) 1063
BshVI ATCGAT 1 cut(s) 78
BsiHKAI GWGCWC 1 cut(s) 1249
BsiSI CCGG 3 cut(s) 213, 243, 1064
BslFI GGGAC 1 cut(s) 982
BslI CCNNNNNNNGG 1 cut(s) 1238
BsmAI GTCTC 3 cut(s) 129, 210, 1261
BsmBI CGTCTC 1 cut(s) 210
BsmFI GGGAC 1 cut(s) 982
BsmI GAATGC 1 cut(s) 1195
BsnI GGCC 2 cut(s) 561, 868
Bso31I GGTCTC 1 cut(s) 1261
Bsp1286I GDGCHC 1 cut(s) 1249
Bsp143I GATC 9 cut(s) 72, 79, 87, 429, 438, 634, 932, 1050, 1227
BspACI CCGC 3 cut(s) 266, 1084, 1256
BspANI GGCC 2 cut(s) 561, 868
BspCNI CTCAG 5 cut(s) 364, 529, 576, 886, 1261
BspDI ATCGAT 1 cut(s) 78
BspLI GGNNCC 1 cut(s) 238
BspPI GGATC 5 cut(s) 80, 437, 642, 1045, 1222
BspTI CTTAAG 2 cut(s) 497, 527
BspTNI GGTCTC 1 cut(s) 1261
BsrFI RCCGGY 1 cut(s) 1063
BsrI ACTGG 1 cut(s) 670
BssAI RCCGGY 1 cut(s) 1063
BssECI CCNNGG 2 cut(s) 232, 801
BssMI GATC 9 cut(s) 72, 79, 87, 429, 438, 634, 932, 1050, 1227
BssSI CACGAG 1 cut(s) 1184
BssT1I CCWWGG 1 cut(s) 801
Bst2BI CACGAG 1 cut(s) 1184
Bst2UI CCWGG 2 cut(s) 1095, 1272
Bst4CI ACNGT 2 cut(s) 233, 1021
Bst6I CTCTTC 3 cut(s) 127, 390, 711
BstAFI CTTAAG 2 cut(s) 497, 527
BstDEI CTNAG 7 cut(s) 372, 414, 537, 563, 600, 894, 1248
BstDSI CCRYGG 1 cut(s) 232
BstF5I GGATG 2 cut(s) 191, 1011
BstKTI GATC 9 cut(s) 75, 82, 90, 432, 441, 637, 935, 1053, 1230
BstMAI GTCTC 3 cut(s) 129, 210, 1261
BstMBI GATC 9 cut(s) 72, 79, 87, 429, 438, 634, 932, 1050, 1227
BstMWI GCNNNNNNNGC 1 cut(s) 186
BstNI CCWGG 2 cut(s) 1095, 1272
BstSCI CCNGG 3 cut(s) 211, 1093, 1270
BstSFI CTRYAG 1 cut(s) 163
BstV1I GCAGC 2 cut(s) 85, 1213
BstV2I GAAGAC 1 cut(s) 982
BstX2I RGATCY 2 cut(s) 438, 932
BstYI RGATCY 2 cut(s) 438, 932
Bsu15I ATCGAT 1 cut(s) 78
Bsu36I CCTNAGG 2 cut(s) 563, 894
BsuI GTATCC 1 cut(s) 906
BsuRI GGCC 2 cut(s) 561, 868
BsuTUI ATCGAT 1 cut(s) 78
BtgI CCRYGG 1 cut(s) 232
BtgZI GCGATG 1 cut(s) 714
BtsCI GGATG 2 cut(s) 191, 1011
BtsIMutI CAGTG 1 cut(s) 663
CaiI CAGNNNCTG 2 cut(s) 689, 956
Cfr10I RCCGGY 1 cut(s) 1063
Cfr13I GGNCC 1 cut(s) 1097
ClaI ATCGAT 1 cut(s) 78
CseI GACGC 2 cut(s) 169, 1142
Csp6I GTAC 5 cut(s) 261, 534, 678, 993, 1217
CspAI ACCGGT 1 cut(s) 1063
CviAII CATG 4 cut(s) 83, 91, 857, 1048
CviQI GTAC 5 cut(s) 261, 534, 678, 993, 1217
DdeI CTNAG 7 cut(s) 372, 414, 537, 563, 600, 894, 1248
DpnI GATC 9 cut(s) 74, 81, 89, 431, 440, 636, 934, 1052, 1229
DpnII GATC 9 cut(s) 72, 79, 87, 429, 438, 634, 932, 1050, 1227
Eam1104I CTCTTC 3 cut(s) 127, 390, 711
EarI CTCTTC 3 cut(s) 127, 390, 711
Eco130I CCWWGG 1 cut(s) 801
Eco31I GGTCTC 1 cut(s) 1261
Eco32I GATATC 1 cut(s) 751
Eco47I GGWCC 1 cut(s) 1097
Eco57I CTGAAG 2 cut(s) 693, 852
Eco81I CCTNAGG 2 cut(s) 563, 894
EcoRII CCWGG 2 cut(s) 1093, 1270
EcoRV GATATC 1 cut(s) 751
EcoT14I CCWWGG 1 cut(s) 801
ErhI CCWWGG 1 cut(s) 801
Esp3I CGTCTC 1 cut(s) 210
FaeI CATG 4 cut(s) 86, 94, 860, 1051
FaiI YATR 9 cut(s) 65, 84, 92, 282, 357, 739, 741, 858, 1049
FalI AAGNNNNNCTT 2 cut(s) 1098, 1130
FaqI GGGAC 1 cut(s) 982
FatI CATG 4 cut(s) 82, 90, 856, 1047
FauNDI CATATG 1 cut(s) 739
Fnu4HI GCNGC 2 cut(s) 99, 1202
FokI GGATG 2 cut(s) 198, 1018
Fsp4HI GCNGC 2 cut(s) 99, 1202
FspBI CTAG 2 cut(s) 426, 972
GluI GCNGC 2 cut(s) 99, 1202
GsuI CTGGAG 1 cut(s) 49
HaeIII GGCC 2 cut(s) 561, 868
HapII CCGG 3 cut(s) 213, 243, 1064
HgaI GACGC 2 cut(s) 169, 1142
Hin1II CATG 4 cut(s) 86, 94, 860, 1051
HinfI GANTC 7 cut(s) 56, 172, 482, 586, 604, 686, 797
HpaII CCGG 3 cut(s) 213, 243, 1064
HphI GGTGA 1 cut(s) 655
Hpy166II GTNNAC 2 cut(s) 680, 1030
Hpy188I TCNGA 8 cut(s) 44, 61, 185, 342, 580, 832, 1039, 1251
Hpy188III TCNNGA 8 cut(s) 70, 129, 197, 565, 1124, 1166, 1184, 1264
Hpy8I GTNNAC 2 cut(s) 680, 1030
Hpy99I CGWCG 3 cut(s) 48, 185, 224
HpyAV CCTTC 3 cut(s) 616, 668, 858
HpyCH4III ACNGT 2 cut(s) 233, 1021
HpyCH4V TGCA 3 cut(s) 692, 1130, 1151
HpyF10VI GCNNNNNNNGC 1 cut(s) 186
HpyF3I CTNAG 7 cut(s) 372, 414, 537, 563, 600, 894, 1248
Hsp92II CATG 4 cut(s) 86, 94, 860, 1051
Kzo9I GATC 9 cut(s) 72, 79, 87, 429, 438, 634, 932, 1050, 1227
LmnI GCTCC 2 cut(s) 928, 1096
Lsp1109I GCAGC 2 cut(s) 85, 1213
LweI GCATC 5 cut(s) 17, 176, 910, 1117, 1189
MaeI CTAG 2 cut(s) 426, 972
MaeIII GTNAC 3 cut(s) 474, 824, 1069
MalI GATC 9 cut(s) 74, 81, 89, 431, 440, 636, 934, 1052, 1229
MboI GATC 9 cut(s) 72, 79, 87, 429, 438, 634, 932, 1050, 1227
MflI RGATCY 2 cut(s) 438, 932
MhlI GDGCHC 1 cut(s) 1249
MluCI AATT 4 cut(s) 119, 200, 352, 1288
MlyI GAGTC 2 cut(s) 598, 695
MmeI TCCRAC 2 cut(s) 67, 163
MseI TTAA 6 cut(s) 488, 498, 528, 555, 939, 1116
MslI CAYNNNNRTG 1 cut(s) 1188
MspCI CTTAAG 2 cut(s) 497, 527
MspI CCGG 3 cut(s) 213, 243, 1064
MspR9I CCNGG 3 cut(s) 213, 1095, 1272
Mva1269I GAATGC 1 cut(s) 1195
MvaI CCWGG 2 cut(s) 1095, 1272
MwoI GCNNNNNNNGC 1 cut(s) 186
NciI CCSGG 1 cut(s) 213
NdeI CATATG 1 cut(s) 739
NdeII GATC 9 cut(s) 72, 79, 87, 429, 438, 634, 932, 1050, 1227
NlaIII CATG 4 cut(s) 86, 94, 860, 1051
NlaIV GGNNCC 1 cut(s) 238
NmeAIII GCCGAG 2 cut(s) 172, 875
PcsI WCGNNNNNNNCGW 1 cut(s) 75
PctI GAATGC 1 cut(s) 1195
PfeI GAWTC 5 cut(s) 56, 172, 482, 586, 797
PfoI TCCNGGA 2 cut(s) 211, 1093
PinAI ACCGGT 1 cut(s) 1063
PkrI GCNGC 2 cut(s) 100, 1203
PleI GAGTC 2 cut(s) 598, 694
PpsI GAGTC 2 cut(s) 598, 694
Psp6I CCWGG 2 cut(s) 1093, 1270
PspGI CCWGG 2 cut(s) 1093, 1270
PspN4I GGNNCC 1 cut(s) 238
PspPI GGNCC 1 cut(s) 1097
PstNI CAGNNNCTG 2 cut(s) 689, 956
PsuI RGATCY 2 cut(s) 438, 932
RsaI GTAC 5 cut(s) 262, 535, 679, 994, 1218
RsaNI GTAC 5 cut(s) 261, 534, 678, 993, 1217
RseI CAYNNNNRTG 1 cut(s) 1188
SaqAI TTAA 6 cut(s) 488, 498, 528, 555, 939, 1116
SatI GCNGC 2 cut(s) 99, 1202
Sau3AI GATC 9 cut(s) 72, 79, 87, 429, 438, 634, 932, 1050, 1227
Sau96I GGNCC 1 cut(s) 1097
ScaI AGTACT 2 cut(s) 535, 1218
SchI GAGTC 2 cut(s) 598, 695
ScrFI CCNGG 3 cut(s) 213, 1095, 1272
SduI GDGCHC 1 cut(s) 1249
SfaNI GCATC 5 cut(s) 17, 176, 910, 1117, 1189
SfcI CTRYAG 1 cut(s) 163
SinI GGWCC 1 cut(s) 1097
SmiMI CAYNNNNRTG 1 cut(s) 1188
SmlI CTYRAG 4 cut(s) 497, 527, 1220, 1262
SmoI CTYRAG 4 cut(s) 497, 527, 1220, 1262
Sse9I AATT 4 cut(s) 119, 200, 352, 1288
SsiI CCGC 3 cut(s) 266, 1084, 1256
SspI AATATT 1 cut(s) 38
SspMI CTAG 2 cut(s) 426, 972
StyD4I CCNGG 3 cut(s) 211, 1093, 1270
StyI CCWWGG 1 cut(s) 801
TaaI ACNGT 2 cut(s) 233, 1021
TaqI TCGA 3 cut(s) 78, 480, 589
TasI AATT 4 cut(s) 119, 200, 352, 1288
TatI WGTACW 3 cut(s) 533, 992, 1216
TfiI GAWTC 5 cut(s) 56, 172, 482, 586, 797
Tru1I TTAA 6 cut(s) 488, 498, 528, 555, 939, 1116
Tru9I TTAA 6 cut(s) 488, 498, 528, 555, 939, 1116
TscAI CASTG 1 cut(s) 670
TseI GCWGC 2 cut(s) 98, 1201
TspDTI ATGAA 7 cut(s) 6, 17, 288, 381, 726, 873, 1022
TspGWI ACGGA 1 cut(s) 786
TspRI CASTG 1 cut(s) 670
Vha464I CTTAAG 2 cut(s) 497, 527
VpaK11BI GGWCC 1 cut(s) 1097
XapI RAATTY 1 cut(s) 119
XspI CTAG 2 cut(s) 426, 972
ZrmI AGTACT 2 cut(s) 535, 1218
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.