Rroxscaffold_2G00097150

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0010201
Physical Location & Seq
Reverse (-)
18418592 .. 18421714
3123 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00097150.1

Sequence Viewer

Length: 1413 bp
ATGGCGACCACGGCCAAGTCTGGGTCTTCACTGAATTACTGGAGGAATTATTTCCGGACGGCGAATTCGGACATTTTCGGCATCATTGACCATGCCATCATGGTTGCCGCTGTGGATTGTCCCAAGGAGTTCAGGTTGCGGAGGGATCGAGTCGCTGAGCTCTTGTTTTCCTGCAGAATGAGCAGGTGTTCGGGTTGCGAAAAGGTGGAGTTGGTGGTGCCTGGTGGTCATGTTCATGGCCATGATGATCACTATGAGGTGGAGGATGATGATGATGATGATGATGATCGAGGTGATAGAGATTTTGAGGCCGGGGCTAGTAAAGAGAGCAAGGCCAATAGCAGCAGAGATTATCAGATTGGTGAGATGAGCATGAAGAATGATCAGGATAGCAATTACAGCTATGGATTGGCTGAGGCTTTGACCGATGAGATTGAACAAGAGTCTCAGGTTTTCGGGGAGGTTTTGAGGATCAAAGAGATCTTCCAGAATAGTGAACATGAGTCTGATGCTGTATTGTTTGAGTCGTTGAGGAAGCTTGAGTTGATGGCTCTGTCTGTGGAGACTCTCAAGGCAACTGAGATTGGCAAGGCTGTTAACCGGCTAAGAAAGCATGGATCAATTAAAATTCGTAATCTTGTTCGAACACTAATTGATGGGTGGAAAGTCATGGTAGATGAATGGGTCAATGCTACTACAGCTATTGCTTCTGGGGGAACAGAAGTTATTTCGGATTCTGTTGATCCATCAGTTGATGATGACTTTGAAGAAGGACTTCCCACTCCTCCGTTGGATGAAGGAGCTTTCTTTGCCACTCAAACCACCTCAATGGAACTCTCTGAGTTCTTTGATGGTATGGAAGATTATGGGAATCCTCGAAACAGTGGACAATATACCAGGAATCGTGAAAATGGAAGAAAACCATCACTGGAGAAACAAAATGTTGTGAAGCAGGAACAACAGTATGTTAGTGATCAGGGAAATGTGCTTCCCAAGAAAATAAAAAGTGAACCAGTGATGGTGAAACAAGAAGTTGATGTGAAGCCAAATAGGCCCTCAAACACCAACTCTGGCCCTCGGAGACCTCCCAAAATAAGTATGGAGCGAAAGGGTGTCAGTAGTGTAACAAATATTGAACAAAGAACAGATAAGGTTTCCATTCAGAAGAGGCAACTCACTGGTCAGCAGCAAGATAAATTCAAGTGTTCAGATGAAGTTGCAGTCCAAGTGAAGCTTGAAGCCACAAAAAGGAAACTCCAGGAGAGTTATCAACAAGCTGAGAATGCCAAGAAGCAACGGACAGTACAGGTAATGGAATTGCATGATCTACCTAAGCAGGGGCTTGGCCATAGGAATCCACATTTAAAACCTGGAAACCACAATCGGCAAAGGGCACATGGGCGGAGATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Med26

Protein Analysis

470

Amino Acids

53.2

Weight (kDa)

5.76

Isoelectric Point (pI)

37.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med26 PF08711 176 - 225 6.5e-13 TFIIS helical bundle-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 174
Acc36I ACCTGC 1 cut(s) 174
AccB1I GGYRCC 1 cut(s) 217
AccIII TCCGGA 1 cut(s) 54
AciI CCGC 3 cut(s) 108, 139, 1402
AclWI GGATC 4 cut(s) 153, 479, 625, 737
AcoI YGGCCR 3 cut(s) 12, 238, 1345
AcsI RAATTY 3 cut(s) 64, 627, 1196
AfaI GTAC 1 cut(s) 1305
AfiI CCNNNNNNNGG 3 cut(s) 21, 1248, 1337
AgsI TTSAA 5 cut(s) 437, 767, 1136, 1201, 1238
AjnI CCWGG 4 cut(s) 220, 896, 1257, 1369
AluBI AGCT 7 cut(s) 160, 402, 538, 701, 803, 1234, 1277
AluI AGCT 7 cut(s) 160, 402, 538, 701, 803, 1234, 1277
Alw21I GWGCWC 1 cut(s) 162
Alw26I GTCTC 3 cut(s) 450, 557, 1075
AlwI GGATC 4 cut(s) 153, 479, 625, 737
Aor13HI TCCGGA 1 cut(s) 54
AoxI GGCC 7 cut(s) 12, 238, 309, 333, 1052, 1072, 1345
ApeKI GCWGC 2 cut(s) 342, 1186
ApoI RAATTY 3 cut(s) 64, 627, 1196
ArsI GACNNNNNNTTYG 2 cut(s) 49, 81
Asp700I GAANNNNTTC 2 cut(s) 50, 774
AspS9I GGNCC 2 cut(s) 1053, 1073
AsuC2I CCSGG 1 cut(s) 313
AsuHPI GGTGA 3 cut(s) 305, 374, 1033
AsuII TTCGAA 1 cut(s) 643
BaeGI GKGCMC 1 cut(s) 1396
BalI TGGCCA 2 cut(s) 240, 1347
BanI GGYRCC 1 cut(s) 217
BanII GRGCYC 1 cut(s) 162
BbsI GAAGAC 1 cut(s) 18
Bbv12I GWGCWC 1 cut(s) 162
BbvCI CCTCAGC 1 cut(s) 414
BbvI GCAGC 2 cut(s) 354, 1198
BccI CCATC 7 cut(s) 104, 541, 650, 754, 845, 931, 1012
BceAI ACGGC 2 cut(s) 27, 75
BciT130I CCWGG 4 cut(s) 222, 898, 1259, 1371
BclI TGATCA 3 cut(s) 247, 382, 973
BcnI CCSGG 1 cut(s) 313
BcoDI GTCTC 3 cut(s) 450, 557, 1075
BfaI CTAG 1 cut(s) 318
BfmI CTRYAG 2 cut(s) 172, 696
BfuAI ACCTGC 1 cut(s) 174
BglI GCCNNNNNGGC 1 cut(s) 1051
BglII AGATCT 1 cut(s) 480
BisI GCNGC 3 cut(s) 108, 343, 1187
BlpI GCTNAGC 1 cut(s) 156
BlsI GCNGC 3 cut(s) 109, 344, 1188
Bme1390I CCNGG 5 cut(s) 222, 313, 898, 1259, 1371
BmgT120I GGNCC 2 cut(s) 1053, 1073
BmiI GGNNCC 1 cut(s) 219
BmrFI CCNGG 5 cut(s) 222, 313, 898, 1259, 1371
BmsI GCATC 2 cut(s) 90, 499
BpiI GAAGAC 1 cut(s) 18
BpmI CTGGAG 3 cut(s) 61, 950, 1241
Bpu10I CCTNAGC 2 cut(s) 414, 1332
Bpu1102I GCTNAGC 1 cut(s) 156
Bpu14I TTCGAA 1 cut(s) 643
BpuEI CTTGAG 2 cut(s) 554, 560
BpuMI CCSGG 1 cut(s) 313
BsaBI GATNNNNATC 1 cut(s) 285
BsaI GGTCTC 1 cut(s) 1075
BsaJI CCNNGG 4 cut(s) 9, 123, 312, 1076
BsaWI WCCGGW 1 cut(s) 54
BsaXI ACNNNNNCTCC 2 cut(s) 119, 149
Bsc4I CCNNNNNNNGG 3 cut(s) 21, 1248, 1337
Bse118I RCCGGY 1 cut(s) 600
Bse1I ACTGG 4 cut(s) 44, 933, 1013, 1183
Bse8I GATNNNNATC 1 cut(s) 285
BseAI TCCGGA 1 cut(s) 54
BseBI CCWGG 4 cut(s) 222, 898, 1259, 1371
BseDI CCNNGG 4 cut(s) 9, 123, 312, 1076
BseGI GGATG 2 cut(s) 271, 799
BseJI GATNNNNATC 1 cut(s) 285
BseLI CCNNNNNNNGG 3 cut(s) 21, 1248, 1337
BseMII CTCAG 6 cut(s) 147, 405, 461, 570, 831, 1269
BseNI ACTGG 4 cut(s) 44, 933, 1013, 1183
BseRI GAGGAG 1 cut(s) 774
BseSI GKGCMC 1 cut(s) 1396
BseXI GCAGC 2 cut(s) 354, 1198
BshFI GGCC 7 cut(s) 14, 240, 311, 335, 1054, 1074, 1347
BshNI GGYRCC 1 cut(s) 217
BsiHKAI GWGCWC 1 cut(s) 162
BsiSI CCGG 3 cut(s) 55, 312, 601
BslFI GGGAC 1 cut(s) 105
BslI CCNNNNNNNGG 3 cut(s) 21, 1248, 1337
BsmAI GTCTC 3 cut(s) 450, 557, 1075
BsmFI GGGAC 1 cut(s) 105
BsmI GAATGC 1 cut(s) 1288
BsnI GGCC 7 cut(s) 14, 240, 311, 335, 1054, 1074, 1347
Bso31I GGTCTC 1 cut(s) 1075
Bsp119I TTCGAA 1 cut(s) 643
Bsp1286I GDGCHC 2 cut(s) 162, 1396
Bsp13I TCCGGA 1 cut(s) 54
Bsp1720I GCTNAGC 1 cut(s) 156
BspACI CCGC 3 cut(s) 108, 139, 1402
BspANI GGCC 7 cut(s) 14, 240, 311, 335, 1054, 1074, 1347
BspCNI CTCAG 6 cut(s) 148, 406, 460, 571, 832, 1270
BspEI TCCGGA 1 cut(s) 54
BspLI GGNNCC 1 cut(s) 219
BspMAI CTGCAG 1 cut(s) 176
BspMI ACCTGC 1 cut(s) 174
BspPI GGATC 4 cut(s) 153, 479, 625, 737
BspT104I TTCGAA 1 cut(s) 643
BspT107I GGYRCC 1 cut(s) 217
BspTNI GGTCTC 1 cut(s) 1075
BsrFI RCCGGY 1 cut(s) 600
BsrI ACTGG 4 cut(s) 44, 933, 1013, 1183
BssAI RCCGGY 1 cut(s) 600
BssECI CCNNGG 4 cut(s) 9, 123, 312, 1076
BssT1I CCWWGG 1 cut(s) 123
Bst2UI CCWGG 4 cut(s) 222, 898, 1259, 1371
Bst4CI ACNGT 3 cut(s) 884, 963, 1303
Bst6I CTCTTC 1 cut(s) 1160
BstBI TTCGAA 1 cut(s) 643
BstDEI CTNAG 8 cut(s) 156, 414, 447, 579, 605, 840, 1278, 1332
BstDSI CCRYGG 1 cut(s) 9
BstF5I GGATG 2 cut(s) 271, 799
BstMAI GTCTC 3 cut(s) 450, 557, 1075
BstMWI GCNNNNNNNGC 8 cut(s) 11, 180, 399, 610, 698, 809, 1051, 1283
BstNI CCWGG 4 cut(s) 222, 898, 1259, 1371
BstSCI CCNGG 5 cut(s) 220, 311, 896, 1257, 1369
BstSFI CTRYAG 2 cut(s) 172, 696
BstSLI GKGCMC 1 cut(s) 1396
BstV1I GCAGC 2 cut(s) 354, 1198
BstV2I GAAGAC 1 cut(s) 18
BstX2I RGATCY 1 cut(s) 480
BstXI CCANNNNNNTGG 1 cut(s) 829
BstYI RGATCY 1 cut(s) 480
BsuRI GGCC 7 cut(s) 14, 240, 311, 335, 1054, 1074, 1347
BtgI CCRYGG 1 cut(s) 9
BtsCI GGATG 2 cut(s) 271, 799
BtsIMutI CAGTG 5 cut(s) 29, 889, 926, 1020, 1176
BveI ACCTGC 1 cut(s) 174
Cfr10I RCCGGY 1 cut(s) 600
Cfr13I GGNCC 2 cut(s) 1053, 1073
Csp6I GTAC 1 cut(s) 1304
CviQI GTAC 1 cut(s) 1304
DdeI CTNAG 8 cut(s) 156, 414, 447, 579, 605, 840, 1278, 1332
DraI TTTAAA 1 cut(s) 1365
EaeI YGGCCR 3 cut(s) 12, 238, 1345
Eam1104I CTCTTC 1 cut(s) 1160
EarI CTCTTC 1 cut(s) 1160
Ecl136II GAGCTC 1 cut(s) 160
Eco130I CCWWGG 1 cut(s) 123
Eco24I GRGCYC 1 cut(s) 162
Eco31I GGTCTC 1 cut(s) 1075
Eco53kI GAGCTC 1 cut(s) 160
EcoICRI GAGCTC 1 cut(s) 160
EcoO109I RGGNCCY 1 cut(s) 1053
EcoRI GAATTC 1 cut(s) 64
EcoRII CCWGG 4 cut(s) 220, 896, 1257, 1369
EcoT14I CCWWGG 1 cut(s) 123
EcoT38I GRGCYC 1 cut(s) 162
ErhI CCWWGG 1 cut(s) 123
FalI AAGNNNNNCTT 4 cut(s) 759, 791, 1218, 1250
FaqI GGGAC 1 cut(s) 105
FbaI TGATCA 3 cut(s) 247, 382, 973
Fnu4HI GCNGC 3 cut(s) 108, 343, 1187
FokI GGATG 2 cut(s) 278, 806
FriOI GRGCYC 1 cut(s) 162
Fsp4HI GCNGC 3 cut(s) 108, 343, 1187
FspBI CTAG 1 cut(s) 318
GluI GCNGC 3 cut(s) 108, 343, 1187
GsuI CTGGAG 3 cut(s) 61, 950, 1241
HaeIII GGCC 7 cut(s) 14, 240, 311, 335, 1054, 1074, 1347
HapII CCGG 3 cut(s) 55, 312, 601
HincII GTYRAC 1 cut(s) 598
HindII GTYRAC 1 cut(s) 598
HindIII AAGCTT 2 cut(s) 536, 1232
HpaI GTTAAC 1 cut(s) 598
HpaII CCGG 3 cut(s) 55, 312, 601
HphI GGTGA 3 cut(s) 305, 374, 1033
Hpy166II GTNNAC 4 cut(s) 497, 598, 887, 1010
Hpy188I TCNGA 9 cut(s) 70, 357, 508, 733, 841, 1080, 1164, 1210, 1412
Hpy188III TCNNGA 4 cut(s) 55, 386, 487, 905
Hpy8I GTNNAC 4 cut(s) 497, 598, 887, 1010
HpyAV CCTTC 2 cut(s) 764, 791
HpyCH4III ACNGT 3 cut(s) 884, 963, 1303
HpyCH4V TGCA 3 cut(s) 174, 1220, 1321
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 180, 399, 610, 698, 809, 1051, 1283
HpyF3I CTNAG 8 cut(s) 156, 414, 447, 579, 605, 840, 1278, 1332
Kpn2I TCCGGA 1 cut(s) 54
Ksp22I TGATCA 3 cut(s) 247, 382, 973
KspAI GTTAAC 1 cut(s) 598
LmnI GCTCC 2 cut(s) 800, 1102
Lsp1109I GCAGC 2 cut(s) 354, 1198
LweI GCATC 2 cut(s) 90, 499
MaeI CTAG 1 cut(s) 318
MaeIII GTNAC 1 cut(s) 1123
MboII GAAGA 7 cut(s) 18, 388, 475, 779, 872, 927, 1177
MflI RGATCY 1 cut(s) 480
MhlI GDGCHC 2 cut(s) 162, 1396
MlsI TGGCCA 2 cut(s) 240, 1347
MluCI AATT 9 cut(s) 34, 46, 64, 394, 621, 627, 651, 1196, 1316
MluNI TGGCCA 2 cut(s) 240, 1347
MlyI GAGTC 5 cut(s) 159, 452, 512, 533, 559
MmeI TCCRAC 1 cut(s) 771
Mox20I TGGCCA 2 cut(s) 240, 1347
MroI TCCGGA 1 cut(s) 54
MroXI GAANNNNTTC 2 cut(s) 50, 774
MscI TGGCCA 2 cut(s) 240, 1347
MseI TTAA 3 cut(s) 597, 624, 1364
MslI CAYNNNNRTG 3 cut(s) 234, 240, 827
Msp20I TGGCCA 2 cut(s) 240, 1347
MspA1I CMGCKG 1 cut(s) 110
MspI CCGG 3 cut(s) 55, 312, 601
MspR9I CCNGG 5 cut(s) 222, 313, 898, 1259, 1371
Mva1269I GAATGC 1 cut(s) 1288
MvaI CCWGG 4 cut(s) 222, 898, 1259, 1371
MwoI GCNNNNNNNGC 8 cut(s) 11, 180, 399, 610, 698, 809, 1051, 1283
NciI CCSGG 1 cut(s) 313
NlaIV GGNNCC 1 cut(s) 219
NspV TTCGAA 1 cut(s) 643
PaqCI CACCTGC 1 cut(s) 174
PctI GAATGC 1 cut(s) 1288
PdmI GAANNNNTTC 2 cut(s) 50, 774
PfeI GAWTC 5 cut(s) 734, 871, 901, 1354, 1407
PfoI TCCNGGA 1 cut(s) 1257
PkrI GCNGC 3 cut(s) 109, 344, 1188
PleI GAGTC 5 cut(s) 158, 451, 511, 532, 559
PpsI GAGTC 5 cut(s) 158, 451, 511, 532, 559
Psp124BI GAGCTC 1 cut(s) 162
Psp6I CCWGG 4 cut(s) 220, 896, 1257, 1369
PspGI CCWGG 4 cut(s) 220, 896, 1257, 1369
PspN4I GGNNCC 1 cut(s) 219
PspPI GGNCC 2 cut(s) 1053, 1073
PstI CTGCAG 1 cut(s) 176
PsuI RGATCY 1 cut(s) 480
RsaI GTAC 1 cut(s) 1305
RsaNI GTAC 1 cut(s) 1304
RseI CAYNNNNRTG 3 cut(s) 234, 240, 827
SacI GAGCTC 1 cut(s) 162
SaqAI TTAA 3 cut(s) 597, 624, 1364
SatI GCNGC 3 cut(s) 108, 343, 1187
Sau96I GGNCC 2 cut(s) 1053, 1073
SchI GAGTC 5 cut(s) 159, 452, 512, 533, 559
ScrFI CCNGG 5 cut(s) 222, 313, 898, 1259, 1371
SduI GDGCHC 2 cut(s) 162, 1396
SfaNI GCATC 2 cut(s) 90, 499
SfcI CTRYAG 2 cut(s) 172, 696
SfuI TTCGAA 1 cut(s) 643
SmiMI CAYNNNNRTG 3 cut(s) 234, 240, 827
SmlI CTYRAG 2 cut(s) 539, 569
SmoI CTYRAG 2 cut(s) 539, 569
Sse9I AATT 9 cut(s) 34, 46, 64, 394, 621, 627, 651, 1196, 1316
SsiI CCGC 3 cut(s) 108, 139, 1402
SspI AATATT 1 cut(s) 1132
SspMI CTAG 1 cut(s) 318
SstI GAGCTC 1 cut(s) 162
StyD4I CCNGG 5 cut(s) 220, 311, 896, 1257, 1369
StyI CCWWGG 1 cut(s) 123
TaaI ACNGT 3 cut(s) 884, 963, 1303
TaqI TCGA 4 cut(s) 148, 289, 643, 877
TaqII GACCGA 1 cut(s) 440
TasI AATT 9 cut(s) 34, 46, 64, 394, 621, 627, 651, 1196, 1316
TatI WGTACW 1 cut(s) 1303
TauI GCSGC 1 cut(s) 110
TfiI GAWTC 5 cut(s) 734, 871, 901, 1354, 1407
Tru1I TTAA 3 cut(s) 597, 624, 1364
Tru9I TTAA 3 cut(s) 597, 624, 1364
TscAI CASTG 5 cut(s) 36, 889, 933, 1020, 1183
TseI GCWGC 2 cut(s) 342, 1186
TspDTI ATGAA 5 cut(s) 224, 389, 693, 810, 1227
TspGWI ACGGA 2 cut(s) 777, 1312
TspRI CASTG 5 cut(s) 36, 889, 933, 1020, 1183
XapI RAATTY 3 cut(s) 64, 627, 1196
XcmI CCANNNNNNNNNTGG 2 cut(s) 787, 1096
XmnI GAANNNNTTC 2 cut(s) 50, 774
XspI CTAG 1 cut(s) 318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.