RLG00000020496

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0010201
Physical Location & Seq
Forward (+)
65630997 .. 65632974
1978 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020496

Sequence Viewer

Length: 1236 bp
ATGAGCAGGTGTTCGGGTTGCGAAAAGGTGGAGTTGGTGGTGCCTGGTGGTCATGGTCATGATGATCACTATAAGGCGGAGGATGATGATGATGATGATGATGATGATCGAGGTGATAGAGATTTTGAGGCCGGGGCTAGTAAAGAGAGCAAGGTCAATAGCAGGAGAGACGATCAGATTGGTGAAATGAGCATGAAGAATGATCAGGATAGCAATTACAGCTATGGATTGGCTGAGGCCTTGACCGATGAGATTGAAGAAGAGTCTCAAGTTATCGGGGAGGTTTTGAGGATCAAAGAGATCTTCCAGAATAGTGAACATGAGTCTGATGCTGTATTGTTTGAGTCGTTGAGGAAGCTTGAGTTGATGGCTCTGTCTGTGGAGACTCTCAAGGCAACTGAGATTGGGAAGGCTGTTAACCGGCTAAGAAAGCATGGATCAATGAAGATTCGTAATCTTGTTCGAACACTAATTGATGGGTGGAAAGTCATGGTAGATGAATGGGTCAATGCTACTACAGCTATTGCTGCTGGGGGAACAGAAGTTATTTCGGATTCTGTTGATCCATCAGTTGATGATGACTTTGAAGAAGGACTTCCCACTCCTCCATTGGATGAAGGAGCTTTCTTTGCCACTCAAACCACCTCAATGGAACTCTCTGAGTTCTTTGATGGTATGGAAGATTATGGGAATCCTCGAAACAGTGGGCAATATACCAGGAATCGTGAAAATGGAAGAAAACCATCACTGGAGAAACAAAATGTTTTGAACCAGGAACAACAGTATGTTAGTGATCAGGGAAATGTGCTTCCCAAGAAAATAAAAAGTGAACCAGTGATGGTGAAACAAGAAGTTGATGTGAAGCCAAGTAGGCCCTCAAACACCAACTCTGGCCCTCGGAGACCTCCCAAAATAAGTATGGAGCGAAAGGGTGTAACTAGTAGTGTAACAAATATTGAACAAAGAACAGATAAGGTTTCCATTCAGAAGAGGCAACTCACTGTTCAGCAGCAAGATAAATACAAGTGTTCAGATGAAGTGGCAGTCCAAGTGAAGCTTGAAGCCACAAAAAGGAAACTCCAGGAGAGTTATCAACAAGCTGAGAATGCCAAGAAGCAACGGACAGTACAGAAGTTGATCCATTGGGTCAGTAGAGTTGGAATTCATACAAAACGACATAAGCTTGATCTGATGAGGAGATCCCCTTTAGCTCATCAGCAGCCAACAGTGATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Med26

Protein Analysis

412

Amino Acids

46.5

Weight (kDa)

5.36

Isoelectric Point (pI)

44.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med26 PF08711 116 - 165 2.1e-13 TFIIS helical bundle-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 40
AciI CCGC 1 cut(s) 77
AclWI GGATC 5 cut(s) 299, 445, 557, 1132, 1194
AcsI RAATTY 1 cut(s) 1161
AfaI GTAC 1 cut(s) 1128
AfiI CCNNNNNNNGG 1 cut(s) 1071
AgsI TTSAA 5 cut(s) 257, 587, 769, 959, 1061
AhlI ACTAGT 1 cut(s) 938
AjnI CCWGG 4 cut(s) 43, 716, 771, 1080
AluBI AGCT 8 cut(s) 222, 358, 521, 623, 1057, 1100, 1183, 1211
AluI AGCT 8 cut(s) 222, 358, 521, 623, 1057, 1100, 1183, 1211
Alw26I GTCTC 4 cut(s) 162, 270, 377, 895
AlwI GGATC 5 cut(s) 299, 445, 557, 1132, 1194
AoxI GGCC 4 cut(s) 129, 237, 872, 892
ApeKI GCWGC 3 cut(s) 527, 1009, 1219
ApoI RAATTY 1 cut(s) 1161
Asp700I GAANNNNTTC 1 cut(s) 594
AspS9I GGNCC 2 cut(s) 873, 893
AsuC2I CCSGG 1 cut(s) 133
AsuHPI GGTGA 3 cut(s) 125, 194, 853
AsuII TTCGAA 1 cut(s) 463
BanI GGYRCC 1 cut(s) 40
BbvCI CCTCAGC 1 cut(s) 234
BbvI GCAGC 3 cut(s) 514, 1021, 1231
BccI CCATC 6 cut(s) 361, 470, 574, 665, 751, 832
BciT130I CCWGG 4 cut(s) 45, 718, 773, 1082
BclI TGATCA 3 cut(s) 64, 202, 793
BcnI CCSGG 1 cut(s) 133
BcoDI GTCTC 4 cut(s) 162, 270, 377, 895
BcuI ACTAGT 1 cut(s) 938
BfaI CTAG 2 cut(s) 138, 939
BfmI CTRYAG 1 cut(s) 516
BglI GCCNNNNNGGC 1 cut(s) 871
BglII AGATCT 1 cut(s) 300
BisI GCNGC 3 cut(s) 528, 1010, 1220
BlsI GCNGC 3 cut(s) 529, 1011, 1221
Bme1390I CCNGG 5 cut(s) 45, 133, 718, 773, 1082
BmgT120I GGNCC 2 cut(s) 873, 893
BmiI GGNNCC 1 cut(s) 42
BmrFI CCNGG 5 cut(s) 45, 133, 718, 773, 1082
BmsI GCATC 1 cut(s) 319
BpmI CTGGAG 2 cut(s) 770, 1064
Bpu10I CCTNAGC 1 cut(s) 234
Bpu14I TTCGAA 1 cut(s) 463
BpuEI CTTGAG 3 cut(s) 252, 374, 380
BpuMI CCSGG 1 cut(s) 133
BsaBI GATNNNNATC 1 cut(s) 105
BsaI GGTCTC 1 cut(s) 895
BsaJI CCNNGG 2 cut(s) 132, 896
Bsc4I CCNNNNNNNGG 1 cut(s) 1071
Bse118I RCCGGY 1 cut(s) 420
Bse1I ACTGG 2 cut(s) 753, 833
Bse8I GATNNNNATC 1 cut(s) 105
BseBI CCWGG 4 cut(s) 45, 718, 773, 1082
BseDI CCNNGG 2 cut(s) 132, 896
BseGI GGATG 2 cut(s) 88, 619
BseJI GATNNNNATC 1 cut(s) 105
BseLI CCNNNNNNNGG 1 cut(s) 1071
BseMII CTCAG 4 cut(s) 225, 390, 651, 1092
BseNI ACTGG 2 cut(s) 753, 833
BseRI GAGGAG 2 cut(s) 594, 1210
BseXI GCAGC 3 cut(s) 514, 1021, 1231
BseYI CCCAGC 1 cut(s) 530
BshFI GGCC 4 cut(s) 131, 239, 874, 894
BshNI GGYRCC 1 cut(s) 40
BsiSI CCGG 2 cut(s) 132, 421
BslI CCNNNNNNNGG 1 cut(s) 1071
BsmAI GTCTC 4 cut(s) 162, 270, 377, 895
BsmBI CGTCTC 1 cut(s) 162
BsmI GAATGC 1 cut(s) 1111
BsnI GGCC 4 cut(s) 131, 239, 874, 894
Bso31I GGTCTC 1 cut(s) 895
Bsp119I TTCGAA 1 cut(s) 463
BspACI CCGC 1 cut(s) 77
BspANI GGCC 4 cut(s) 131, 239, 874, 894
BspCNI CTCAG 4 cut(s) 226, 391, 652, 1093
BspHI TCATGA 1 cut(s) 58
BspLI GGNNCC 1 cut(s) 42
BspPI GGATC 5 cut(s) 299, 445, 557, 1132, 1194
BspT104I TTCGAA 1 cut(s) 463
BspT107I GGYRCC 1 cut(s) 40
BspTNI GGTCTC 1 cut(s) 895
BsrFI RCCGGY 1 cut(s) 420
BsrI ACTGG 2 cut(s) 753, 833
BssAI RCCGGY 1 cut(s) 420
BssECI CCNNGG 2 cut(s) 132, 896
Bst2UI CCWGG 4 cut(s) 45, 718, 773, 1082
Bst4CI ACNGT 5 cut(s) 704, 783, 1003, 1126, 1228
Bst6I CTCTTC 2 cut(s) 255, 983
BstBI TTCGAA 1 cut(s) 463
BstDEI CTNAG 5 cut(s) 234, 399, 425, 660, 1101
BstF5I GGATG 2 cut(s) 88, 619
BstMAI GTCTC 4 cut(s) 162, 270, 377, 895
BstMWI GCNNNNNNNGC 7 cut(s) 219, 430, 518, 527, 629, 871, 1106
BstNI CCWGG 4 cut(s) 45, 718, 773, 1082
BstSCI CCNGG 5 cut(s) 43, 131, 716, 771, 1080
BstSFI CTRYAG 1 cut(s) 516
BstV1I GCAGC 3 cut(s) 514, 1021, 1231
BstX2I RGATCY 2 cut(s) 300, 1199
BstXI CCANNNNNNTGG 1 cut(s) 649
BstYI RGATCY 2 cut(s) 300, 1199
BsuRI GGCC 4 cut(s) 131, 239, 874, 894
BtsCI GGATG 2 cut(s) 88, 619
BtsIMutI CAGTG 5 cut(s) 709, 746, 840, 999, 1233
CciI TCATGA 1 cut(s) 58
Cfr10I RCCGGY 1 cut(s) 420
Cfr13I GGNCC 2 cut(s) 873, 893
Csp6I GTAC 1 cut(s) 1127
CviAII CATG 6 cut(s) 53, 59, 193, 320, 434, 490
CviQI GTAC 1 cut(s) 1127
DdeI CTNAG 5 cut(s) 234, 399, 425, 660, 1101
Eam1104I CTCTTC 2 cut(s) 255, 983
EarI CTCTTC 2 cut(s) 255, 983
EciI GGCGGA 1 cut(s) 92
Eco147I AGGCCT 1 cut(s) 239
Eco31I GGTCTC 1 cut(s) 895
EcoO109I RGGNCCY 1 cut(s) 873
EcoRI GAATTC 1 cut(s) 1161
EcoRII CCWGG 4 cut(s) 43, 716, 771, 1080
Esp3I CGTCTC 1 cut(s) 162
FaeI CATG 6 cut(s) 56, 62, 196, 323, 437, 493
FalI AAGNNNNNCTT 4 cut(s) 579, 611, 1041, 1073
FatI CATG 6 cut(s) 52, 58, 192, 319, 433, 489
FbaI TGATCA 3 cut(s) 64, 202, 793
Fnu4HI GCNGC 3 cut(s) 528, 1010, 1220
FokI GGATG 2 cut(s) 95, 626
Fsp4HI GCNGC 3 cut(s) 528, 1010, 1220
FspBI CTAG 2 cut(s) 138, 939
GluI GCNGC 3 cut(s) 528, 1010, 1220
GsaI CCCAGC 1 cut(s) 534
GsuI CTGGAG 2 cut(s) 770, 1064
HaeIII GGCC 4 cut(s) 131, 239, 874, 894
HapII CCGG 2 cut(s) 132, 421
Hin1II CATG 6 cut(s) 56, 62, 196, 323, 437, 493
HincII GTYRAC 1 cut(s) 418
HindII GTYRAC 1 cut(s) 418
HindIII AAGCTT 3 cut(s) 356, 1055, 1181
HinfI GANTC 8 cut(s) 263, 323, 344, 385, 448, 554, 691, 721
HpaI GTTAAC 1 cut(s) 418
HpaII CCGG 2 cut(s) 132, 421
HphI GGTGA 3 cut(s) 125, 194, 853
Hpy166II GTNNAC 3 cut(s) 317, 418, 830
Hpy188I TCNGA 8 cut(s) 177, 328, 553, 661, 900, 987, 1033, 1191
Hpy188III TCNNGA 4 cut(s) 59, 206, 307, 725
Hpy8I GTNNAC 3 cut(s) 317, 418, 830
HpyAV CCTTC 3 cut(s) 403, 584, 611
HpyCH4III ACNGT 5 cut(s) 704, 783, 1003, 1126, 1228
HpyF10VI GCNNNNNNNGC 7 cut(s) 219, 430, 518, 527, 629, 871, 1106
HpyF3I CTNAG 5 cut(s) 234, 399, 425, 660, 1101
Hsp92II CATG 6 cut(s) 56, 62, 196, 323, 437, 493
Ksp22I TGATCA 3 cut(s) 64, 202, 793
KspAI GTTAAC 1 cut(s) 418
LmnI GCTCC 2 cut(s) 620, 922
Lsp1109I GCAGC 3 cut(s) 514, 1021, 1231
LweI GCATC 1 cut(s) 319
MaeI CTAG 2 cut(s) 138, 939
MaeIII GTNAC 2 cut(s) 934, 946
MboII GAAGA 9 cut(s) 208, 269, 272, 295, 457, 599, 692, 747, 1000
MflI RGATCY 2 cut(s) 300, 1199
MluCI AATT 3 cut(s) 214, 471, 1161
MlyI GAGTC 4 cut(s) 272, 332, 353, 379
MmeI TCCRAC 1 cut(s) 1138
MroXI GAANNNNTTC 1 cut(s) 594
MseI TTAA 1 cut(s) 417
MslI CAYNNNNRTG 2 cut(s) 57, 647
MspI CCGG 2 cut(s) 132, 421
MspR9I CCNGG 5 cut(s) 45, 133, 718, 773, 1082
Mva1269I GAATGC 1 cut(s) 1111
MvaI CCWGG 4 cut(s) 45, 718, 773, 1082
MwoI GCNNNNNNNGC 7 cut(s) 219, 430, 518, 527, 629, 871, 1106
NciI CCSGG 1 cut(s) 133
NlaIII CATG 6 cut(s) 56, 62, 196, 323, 437, 493
NlaIV GGNNCC 1 cut(s) 42
NspV TTCGAA 1 cut(s) 463
PagI TCATGA 1 cut(s) 58
PceI AGGCCT 1 cut(s) 239
PctI GAATGC 1 cut(s) 1111
PdmI GAANNNNTTC 1 cut(s) 594
PfeI GAWTC 4 cut(s) 448, 554, 691, 721
PfoI TCCNGGA 1 cut(s) 1080
PkrI GCNGC 3 cut(s) 529, 1011, 1221
PleI GAGTC 4 cut(s) 271, 331, 352, 379
PpsI GAGTC 4 cut(s) 271, 331, 352, 379
Psp6I CCWGG 4 cut(s) 43, 716, 771, 1080
PspFI CCCAGC 1 cut(s) 530
PspGI CCWGG 4 cut(s) 43, 716, 771, 1080
PspN4I GGNNCC 1 cut(s) 42
PspPI GGNCC 2 cut(s) 873, 893
PsuI RGATCY 2 cut(s) 300, 1199
RsaI GTAC 1 cut(s) 1128
RsaNI GTAC 1 cut(s) 1127
RseI CAYNNNNRTG 2 cut(s) 57, 647
SaqAI TTAA 1 cut(s) 417
SatI GCNGC 3 cut(s) 528, 1010, 1220
Sau96I GGNCC 2 cut(s) 873, 893
SchI GAGTC 4 cut(s) 272, 332, 353, 379
ScrFI CCNGG 5 cut(s) 45, 133, 718, 773, 1082
SfaNI GCATC 1 cut(s) 319
SfcI CTRYAG 1 cut(s) 516
SfuI TTCGAA 1 cut(s) 463
SmiMI CAYNNNNRTG 2 cut(s) 57, 647
SmlI CTYRAG 3 cut(s) 267, 359, 389
SmoI CTYRAG 3 cut(s) 267, 359, 389
SpeI ACTAGT 1 cut(s) 938
Sse9I AATT 3 cut(s) 214, 471, 1161
SseBI AGGCCT 1 cut(s) 239
SsiI CCGC 1 cut(s) 77
SspI AATATT 1 cut(s) 955
SspMI CTAG 2 cut(s) 138, 939
StuI AGGCCT 1 cut(s) 239
StyD4I CCNGG 5 cut(s) 43, 131, 716, 771, 1080
TaaI ACNGT 5 cut(s) 704, 783, 1003, 1126, 1228
TaqI TCGA 3 cut(s) 109, 463, 697
TaqII GACCGA 1 cut(s) 260
TasI AATT 3 cut(s) 214, 471, 1161
TatI WGTACW 1 cut(s) 1126
TfiI GAWTC 4 cut(s) 448, 554, 691, 721
Tru1I TTAA 1 cut(s) 417
Tru9I TTAA 1 cut(s) 417
TscAI CASTG 5 cut(s) 709, 753, 840, 1006, 1233
TseI GCWGC 3 cut(s) 527, 1009, 1219
TspDTI ATGAA 6 cut(s) 209, 458, 513, 630, 1050, 1154
TspGWI ACGGA 1 cut(s) 1135
TspRI CASTG 5 cut(s) 709, 753, 840, 1006, 1233
XapI RAATTY 1 cut(s) 1161
XcmI CCANNNNNNNNNTGG 2 cut(s) 607, 916
XmnI GAANNNNTTC 1 cut(s) 594
XspI CTAG 2 cut(s) 138, 939
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.