RchiOBHm_Chr2g0150411

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0010201
Physical Location & Seq
Forward (+)
68074177 .. 68077240
3064 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ51975

Sequence Viewer

Length: 1419 bp
ATGGCGACCACGGCCAAGTCTGGGTCTTCACTGAATTACTGGAGGAATTATTTCCGGACGGCGAATTCGGACATTTTCGGCATCATTGACCATGCCATCATGGTTGCCGCTGTGGATTGTCCCAAGGAGTTCAGGTTGCGGAGGGATCGAGTTGCTGAGCTCTTGTTTTCCTGCAGAATGAGCAGGTGTTCGGGTTGCGAAAAGGTGGAGTTGGTGGTGCCTGGTGGTCATGGTCATGGTCATGATGATCACTATGAGGTGGAGCATGATGATGATGATGATGATGATGACCGAGGAGGTGATAGAGATTTTGAGGCCGGGGCTAGTAAAGAGAGCAAGGTCAATAGCAGCAGAGATGATCAGATTGGTGAAATGAGCATGAAGAATGATCAGGATAGCAATTACAGCTATGGATTGGCTGAGGCTTTGACCGATGAGATTGAACAAGAGTCTCAGGTTTTCGGGGAGGTTTTGAGGATCAAAGGGATCTTCCAGAATAGTGAACATGAGTCTGATGCTGTATTGTTTGAGTCGTTGAGGAAGCTTGAGTTGATGGCTCTGTCTGTGGAGACTCTCAAGGCAACTGAGATTGGGAAGGCTGTTAACCGGCTAAGAAAGCATGGATCAATGAAGATTCGTAATCTTGTTCGAACACTAATTGATGGGTGGAAAGTCATGGTAGATGAATGGGTCAATGCTACTACAGCTATTGCTTCTGGGGGAACAGAAGTTATTTCAGATTCTGTTGATCCATCAATTGATGATGACTTTGAAGAAGGACTTCCCACTCCTCCATTGGATGAAGGAGCTTTCTTTGCCACTCAAACCACCTCAATGGAACTCTCTGAGTTCTTTGATGGTATGGAAGATTATGGGAATCCTCGAAACAGTGGGCAATATACCAGGAATCGTGAAAATGGAAGAAAACCATCACTGGAGAAACAAAATGTTGTGAAGCAGGAACAACAGTATGTTAGTGATCAGGGAAATGTGCTTCCCAAGAAAATAAAAAGTGAACCAGTGATGGTGAAACAAGAAGTTGATGTGAAGCCAAATAGGCCCTCAAACACCAACTCTGGCCCTCGGAGACCTCCCAAAGTAAGTATGGAGCGAAAGGGTGTCAGTAGTGTAACAAATATTGAGCAAAGAACAGATAAGGTTTCCATTCAGAAGAGGCAACTCACTGGTCAGCAGCAAGATAAATACAAGTGTTCAGATGAAGTTGCAGTGCAAGTGAAGCTTGAAGCCACAAAAAGGAAACTCCAGGAGAGTTATCAACAAGCTGAGAATGCCAAGAAGCAACGGACAGTACAGGTAATGGAATTGCATGATCTACCTAAGCAGGGGCTTGGCCATAGGAACCCACATTTAAAACCTGGAAACCACAATCGGCATAGGGCACATGGGCGGAGATTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Med26

Protein Analysis

472

Amino Acids

53.27

Weight (kDa)

5.86

Isoelectric Point (pI)

36.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med26 PF08711 178 - 227 2.5e-13 TFIIS helical bundle-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 174
Acc36I ACCTGC 1 cut(s) 174
AccB1I GGYRCC 1 cut(s) 217
AccIII TCCGGA 1 cut(s) 54
AciI CCGC 3 cut(s) 108, 139, 1408
AclWI GGATC 5 cut(s) 153, 485, 494, 631, 743
AcoI YGGCCR 2 cut(s) 12, 1351
AcsI RAATTY 1 cut(s) 64
AfaI GTAC 1 cut(s) 1311
AfiI CCNNNNNNNGG 3 cut(s) 21, 1254, 1343
AgsI TTSAA 3 cut(s) 443, 773, 1244
AjnI CCWGG 4 cut(s) 220, 902, 1263, 1375
AluBI AGCT 7 cut(s) 160, 408, 544, 707, 809, 1240, 1283
AluI AGCT 7 cut(s) 160, 408, 544, 707, 809, 1240, 1283
Alw21I GWGCWC 1 cut(s) 162
Alw26I GTCTC 3 cut(s) 456, 563, 1081
AlwI GGATC 5 cut(s) 153, 485, 494, 631, 743
AlwNI CAGNNNCTG 1 cut(s) 743
Aor13HI TCCGGA 1 cut(s) 54
AoxI GGCC 5 cut(s) 12, 315, 1058, 1078, 1351
ApeKI GCWGC 2 cut(s) 348, 1192
ApoI RAATTY 1 cut(s) 64
ArsI GACNNNNNNTTYG 2 cut(s) 49, 81
Asp700I GAANNNNTTC 2 cut(s) 50, 780
AspS9I GGNCC 2 cut(s) 1059, 1079
AsuC2I CCSGG 1 cut(s) 319
AsuHPI GGTGA 3 cut(s) 311, 380, 1039
AsuII TTCGAA 1 cut(s) 649
BaeGI GKGCMC 1 cut(s) 1402
BalI TGGCCA 1 cut(s) 1353
BanI GGYRCC 1 cut(s) 217
BanII GRGCYC 1 cut(s) 162
BbsI GAAGAC 1 cut(s) 18
Bbv12I GWGCWC 1 cut(s) 162
BbvCI CCTCAGC 1 cut(s) 420
BbvI GCAGC 2 cut(s) 360, 1204
BccI CCATC 7 cut(s) 104, 547, 656, 760, 851, 937, 1018
BceAI ACGGC 2 cut(s) 27, 75
BciT130I CCWGG 4 cut(s) 222, 904, 1265, 1377
BclI TGATCA 4 cut(s) 247, 358, 388, 979
BcnI CCSGG 1 cut(s) 319
BcoDI GTCTC 3 cut(s) 456, 563, 1081
BfaI CTAG 1 cut(s) 324
BfmI CTRYAG 2 cut(s) 172, 702
BfuAI ACCTGC 1 cut(s) 174
BglI GCCNNNNNGGC 1 cut(s) 1057
BisI GCNGC 3 cut(s) 108, 349, 1193
BlpI GCTNAGC 1 cut(s) 156
BlsI GCNGC 3 cut(s) 109, 350, 1194
Bme1390I CCNGG 5 cut(s) 222, 319, 904, 1265, 1377
BmgT120I GGNCC 2 cut(s) 1059, 1079
BmiI GGNNCC 2 cut(s) 219, 1361
BmrFI CCNGG 5 cut(s) 222, 319, 904, 1265, 1377
BmsI GCATC 2 cut(s) 90, 505
BpiI GAAGAC 1 cut(s) 18
BpmI CTGGAG 3 cut(s) 61, 956, 1247
Bpu10I CCTNAGC 2 cut(s) 420, 1338
Bpu1102I GCTNAGC 1 cut(s) 156
Bpu14I TTCGAA 1 cut(s) 649
BpuEI CTTGAG 2 cut(s) 560, 566
BpuMI CCSGG 1 cut(s) 319
BsaI GGTCTC 1 cut(s) 1081
BsaJI CCNNGG 5 cut(s) 9, 123, 292, 318, 1082
BsaWI WCCGGW 1 cut(s) 54
BsaXI ACNNNNNCTCC 2 cut(s) 119, 149
Bsc4I CCNNNNNNNGG 3 cut(s) 21, 1254, 1343
Bse118I RCCGGY 1 cut(s) 606
Bse1I ACTGG 4 cut(s) 44, 939, 1019, 1189
BseAI TCCGGA 1 cut(s) 54
BseBI CCWGG 4 cut(s) 222, 904, 1265, 1377
BseDI CCNNGG 5 cut(s) 9, 123, 292, 318, 1082
BseGI GGATG 1 cut(s) 805
BseLI CCNNNNNNNGG 3 cut(s) 21, 1254, 1343
BseMII CTCAG 6 cut(s) 147, 411, 467, 576, 837, 1275
BseNI ACTGG 4 cut(s) 44, 939, 1019, 1189
BseRI GAGGAG 2 cut(s) 309, 780
BseSI GKGCMC 1 cut(s) 1402
BseXI GCAGC 2 cut(s) 360, 1204
BshFI GGCC 5 cut(s) 14, 317, 1060, 1080, 1353
BshNI GGYRCC 1 cut(s) 217
BsiHKAI GWGCWC 1 cut(s) 162
BsiSI CCGG 3 cut(s) 55, 318, 607
BslFI GGGAC 1 cut(s) 105
BslI CCNNNNNNNGG 3 cut(s) 21, 1254, 1343
BsmAI GTCTC 3 cut(s) 456, 563, 1081
BsmFI GGGAC 1 cut(s) 105
BsmI GAATGC 1 cut(s) 1294
BsnI GGCC 5 cut(s) 14, 317, 1060, 1080, 1353
Bso31I GGTCTC 1 cut(s) 1081
Bsp119I TTCGAA 1 cut(s) 649
Bsp1286I GDGCHC 2 cut(s) 162, 1402
Bsp13I TCCGGA 1 cut(s) 54
Bsp1720I GCTNAGC 1 cut(s) 156
BspACI CCGC 3 cut(s) 108, 139, 1408
BspANI GGCC 5 cut(s) 14, 317, 1060, 1080, 1353
BspCNI CTCAG 6 cut(s) 148, 412, 466, 577, 838, 1276
BspEI TCCGGA 1 cut(s) 54
BspHI TCATGA 1 cut(s) 241
BspLI GGNNCC 2 cut(s) 219, 1361
BspMAI CTGCAG 1 cut(s) 176
BspMI ACCTGC 1 cut(s) 174
BspPI GGATC 5 cut(s) 153, 485, 494, 631, 743
BspT104I TTCGAA 1 cut(s) 649
BspT107I GGYRCC 1 cut(s) 217
BspTNI GGTCTC 1 cut(s) 1081
BsrFI RCCGGY 1 cut(s) 606
BsrI ACTGG 4 cut(s) 44, 939, 1019, 1189
BssAI RCCGGY 1 cut(s) 606
BssECI CCNNGG 5 cut(s) 9, 123, 292, 318, 1082
BssT1I CCWWGG 1 cut(s) 123
Bst2UI CCWGG 4 cut(s) 222, 904, 1265, 1377
Bst4CI ACNGT 3 cut(s) 890, 969, 1309
Bst6I CTCTTC 1 cut(s) 1166
BstBI TTCGAA 1 cut(s) 649
BstDEI CTNAG 8 cut(s) 156, 420, 453, 585, 611, 846, 1284, 1338
BstDSI CCRYGG 1 cut(s) 9
BstF5I GGATG 1 cut(s) 805
BstMAI GTCTC 3 cut(s) 456, 563, 1081
BstMWI GCNNNNNNNGC 9 cut(s) 11, 180, 405, 616, 704, 815, 1057, 1237, 1289
BstNI CCWGG 4 cut(s) 222, 904, 1265, 1377
BstSCI CCNGG 5 cut(s) 220, 317, 902, 1263, 1375
BstSFI CTRYAG 2 cut(s) 172, 702
BstSLI GKGCMC 1 cut(s) 1402
BstV1I GCAGC 2 cut(s) 360, 1204
BstV2I GAAGAC 1 cut(s) 18
BstX2I RGATCY 1 cut(s) 486
BstXI CCANNNNNNTGG 1 cut(s) 835
BstYI RGATCY 1 cut(s) 486
BsuRI GGCC 5 cut(s) 14, 317, 1060, 1080, 1353
BtgI CCRYGG 1 cut(s) 9
BtsCI GGATG 1 cut(s) 805
BtsI GCAGTG 1 cut(s) 1233
BtsIMutI CAGTG 6 cut(s) 29, 895, 932, 1026, 1182, 1233
BveI ACCTGC 1 cut(s) 174
CaiI CAGNNNCTG 1 cut(s) 743
CciI TCATGA 1 cut(s) 241
Cfr10I RCCGGY 1 cut(s) 606
Cfr13I GGNCC 2 cut(s) 1059, 1079
Csp6I GTAC 1 cut(s) 1310
CviQI GTAC 1 cut(s) 1310
DdeI CTNAG 8 cut(s) 156, 420, 453, 585, 611, 846, 1284, 1338
DraI TTTAAA 1 cut(s) 1371
EaeI YGGCCR 2 cut(s) 12, 1351
Eam1104I CTCTTC 1 cut(s) 1166
EarI CTCTTC 1 cut(s) 1166
Ecl136II GAGCTC 1 cut(s) 160
Eco130I CCWWGG 1 cut(s) 123
Eco24I GRGCYC 1 cut(s) 162
Eco31I GGTCTC 1 cut(s) 1081
Eco53kI GAGCTC 1 cut(s) 160
EcoICRI GAGCTC 1 cut(s) 160
EcoO109I RGGNCCY 1 cut(s) 1059
EcoRI GAATTC 1 cut(s) 64
EcoRII CCWGG 4 cut(s) 220, 902, 1263, 1375
EcoT14I CCWWGG 1 cut(s) 123
EcoT38I GRGCYC 1 cut(s) 162
ErhI CCWWGG 1 cut(s) 123
FalI AAGNNNNNCTT 4 cut(s) 765, 797, 1224, 1256
FaqI GGGAC 1 cut(s) 105
FbaI TGATCA 4 cut(s) 247, 358, 388, 979
Fnu4HI GCNGC 3 cut(s) 108, 349, 1193
FokI GGATG 1 cut(s) 812
FriOI GRGCYC 1 cut(s) 162
Fsp4HI GCNGC 3 cut(s) 108, 349, 1193
FspBI CTAG 1 cut(s) 324
GluI GCNGC 3 cut(s) 108, 349, 1193
GsuI CTGGAG 3 cut(s) 61, 956, 1247
HaeIII GGCC 5 cut(s) 14, 317, 1060, 1080, 1353
HapII CCGG 3 cut(s) 55, 318, 607
HincII GTYRAC 1 cut(s) 604
HindII GTYRAC 1 cut(s) 604
HindIII AAGCTT 2 cut(s) 542, 1238
HinfI GANTC 8 cut(s) 449, 509, 530, 571, 634, 740, 877, 907
HpaI GTTAAC 1 cut(s) 604
HpaII CCGG 3 cut(s) 55, 318, 607
HphI GGTGA 3 cut(s) 311, 380, 1039
Hpy166II GTNNAC 3 cut(s) 503, 604, 1016
Hpy188I TCNGA 8 cut(s) 70, 363, 514, 739, 847, 1086, 1170, 1216
Hpy188III TCNNGA 5 cut(s) 55, 242, 392, 493, 911
Hpy8I GTNNAC 3 cut(s) 503, 604, 1016
HpyAV CCTTC 3 cut(s) 589, 770, 797
HpyCH4III ACNGT 3 cut(s) 890, 969, 1309
HpyCH4V TGCA 4 cut(s) 174, 1226, 1231, 1327
HpyF10VI GCNNNNNNNGC 9 cut(s) 11, 180, 405, 616, 704, 815, 1057, 1237, 1289
HpyF3I CTNAG 8 cut(s) 156, 420, 453, 585, 611, 846, 1284, 1338
Kpn2I TCCGGA 1 cut(s) 54
Ksp22I TGATCA 4 cut(s) 247, 358, 388, 979
KspAI GTTAAC 1 cut(s) 604
LmnI GCTCC 3 cut(s) 262, 806, 1108
Lsp1109I GCAGC 2 cut(s) 360, 1204
LweI GCATC 2 cut(s) 90, 505
MaeI CTAG 1 cut(s) 324
MaeIII GTNAC 1 cut(s) 1129
MboII GAAGA 8 cut(s) 18, 394, 481, 643, 785, 878, 933, 1183
MfeI CAATTG 1 cut(s) 756
MflI RGATCY 1 cut(s) 486
MhlI GDGCHC 2 cut(s) 162, 1402
MlsI TGGCCA 1 cut(s) 1353
MluCI AATT 7 cut(s) 34, 46, 64, 400, 657, 756, 1322
MluNI TGGCCA 1 cut(s) 1353
MlyI GAGTC 4 cut(s) 458, 518, 539, 565
Mox20I TGGCCA 1 cut(s) 1353
MroI TCCGGA 1 cut(s) 54
MroXI GAANNNNTTC 2 cut(s) 50, 780
MscI TGGCCA 1 cut(s) 1353
MseI TTAA 2 cut(s) 603, 1370
MslI CAYNNNNRTG 4 cut(s) 234, 240, 270, 833
Msp20I TGGCCA 1 cut(s) 1353
MspA1I CMGCKG 1 cut(s) 110
MspI CCGG 3 cut(s) 55, 318, 607
MspR9I CCNGG 5 cut(s) 222, 319, 904, 1265, 1377
MunI CAATTG 1 cut(s) 756
Mva1269I GAATGC 1 cut(s) 1294
MvaI CCWGG 4 cut(s) 222, 904, 1265, 1377
MwoI GCNNNNNNNGC 9 cut(s) 11, 180, 405, 616, 704, 815, 1057, 1237, 1289
NciI CCSGG 1 cut(s) 319
NlaIV GGNNCC 2 cut(s) 219, 1361
NspV TTCGAA 1 cut(s) 649
PagI TCATGA 1 cut(s) 241
PaqCI CACCTGC 1 cut(s) 174
PctI GAATGC 1 cut(s) 1294
PdmI GAANNNNTTC 2 cut(s) 50, 780
PfeI GAWTC 4 cut(s) 634, 740, 877, 907
PfoI TCCNGGA 1 cut(s) 1263
PkrI GCNGC 3 cut(s) 109, 350, 1194
PleI GAGTC 4 cut(s) 457, 517, 538, 565
PpsI GAGTC 4 cut(s) 457, 517, 538, 565
Psp124BI GAGCTC 1 cut(s) 162
Psp6I CCWGG 4 cut(s) 220, 902, 1263, 1375
PspGI CCWGG 4 cut(s) 220, 902, 1263, 1375
PspN4I GGNNCC 2 cut(s) 219, 1361
PspPI GGNCC 2 cut(s) 1059, 1079
PstI CTGCAG 1 cut(s) 176
PstNI CAGNNNCTG 1 cut(s) 743
PsuI RGATCY 1 cut(s) 486
RsaI GTAC 1 cut(s) 1311
RsaNI GTAC 1 cut(s) 1310
RseI CAYNNNNRTG 4 cut(s) 234, 240, 270, 833
SacI GAGCTC 1 cut(s) 162
SaqAI TTAA 2 cut(s) 603, 1370
SatI GCNGC 3 cut(s) 108, 349, 1193
Sau96I GGNCC 2 cut(s) 1059, 1079
SchI GAGTC 4 cut(s) 458, 518, 539, 565
ScrFI CCNGG 5 cut(s) 222, 319, 904, 1265, 1377
SduI GDGCHC 2 cut(s) 162, 1402
SfaNI GCATC 2 cut(s) 90, 505
SfcI CTRYAG 2 cut(s) 172, 702
SfuI TTCGAA 1 cut(s) 649
SmiMI CAYNNNNRTG 4 cut(s) 234, 240, 270, 833
SmlI CTYRAG 2 cut(s) 545, 575
SmoI CTYRAG 2 cut(s) 545, 575
Sse9I AATT 7 cut(s) 34, 46, 64, 400, 657, 756, 1322
SsiI CCGC 3 cut(s) 108, 139, 1408
SspI AATATT 1 cut(s) 1138
SspMI CTAG 1 cut(s) 324
SstI GAGCTC 1 cut(s) 162
StyD4I CCNGG 5 cut(s) 220, 317, 902, 1263, 1375
StyI CCWWGG 1 cut(s) 123
TaaI ACNGT 3 cut(s) 890, 969, 1309
TaqI TCGA 3 cut(s) 148, 649, 883
TaqII GACCGA 2 cut(s) 306, 446
TasI AATT 7 cut(s) 34, 46, 64, 400, 657, 756, 1322
TatI WGTACW 1 cut(s) 1309
TauI GCSGC 1 cut(s) 110
TfiI GAWTC 4 cut(s) 634, 740, 877, 907
Tru1I TTAA 2 cut(s) 603, 1370
Tru9I TTAA 2 cut(s) 603, 1370
TscAI CASTG 6 cut(s) 36, 895, 939, 1026, 1189, 1233
TseI GCWGC 2 cut(s) 348, 1192
TspDTI ATGAA 5 cut(s) 395, 644, 699, 816, 1233
TspGWI ACGGA 1 cut(s) 1318
TspRI CASTG 6 cut(s) 36, 895, 939, 1026, 1189, 1233
XapI RAATTY 1 cut(s) 64
XcmI CCANNNNNNNNNTGG 2 cut(s) 793, 1102
XmnI GAANNNNTTC 2 cut(s) 50, 780
XspI CTAG 1 cut(s) 324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.