AT3G12040

Methylpurine-DNA glycosylase (MPG)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0008717
Physical Location & Seq
Reverse (-)
3835129 .. 3836849
1721 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G12040.1

Sequence Viewer

Length: 765 bp
ATGAAAACGCCGGCTCGTCGATCGAAGCGGGTTAATCAGGAGGAATCGGAGACAAATGTTACAACCCGAGTAGTCCTACGGACACGAAAGACCAACTGTTCCAAGACACGAGCGGCTCGGGTCAGACCCGATTACCCACTGACCCGCACCACCTCCGAGTCCGAGATGAAGTTAATGCCTCCGGAGTTCTTTCAGATCGACGCACTTGATCTTGCGCCACGTTTGCTTGGCAAGTTCATGAGGAGAGACAATGTTGTGCTTCGAATTACAGAGGTAGAAGCTTATAGACCAAATGATTCAGCTTGCCATGGACGATTTGGTGTTACCCCACGGACCGCACCAGTTTTTGGACCAGGAGGACATGCTTATGTTTATCTATGTTATGGTCTTCATATGATGCTCAATATTGTTGCTGATAAAGAAGGAGTCGGAGCTGCTGTTTTGATACGGTCTTGTTCTCCTGTTTCCGGAATGGAGACTATACAGGAGCGTCGTGGCCTGAAAACCGACAAACCTGTTCTTTTAAATGGACCAGGAAAGGTTGGTCAAGCGCTTGGACTCTCAACAGAGTGGTCTCATCATCCACTTTATTCTCCTGGAGGACTGGAGCTTCTGGATGGAGGAGAAGATGTGGAGAAAGTAATGGTTGGTCCTCGCGTTGGGATAGATTACGCATTGCCTGAACATGTGAATGCCTTGTGGAGATTTGCTGTTGCAGACACTCCATGGATAAGTGCTCCTAAGAACACTCTTAAACCTCTCTAA

Protein Analysis

254

Amino Acids

28.13

Weight (kDa)

9.41

Isoelectric Point (pI)

41.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pur_DNA_glyco PF02245 62 - 241 5.5e-53 Methylpurine-DNA glycosylase (MPG)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 347
AccBSI CCGCTC 1 cut(s) 113
AccII CGCG 1 cut(s) 657
AccIII TCCGGA 2 cut(s) 181, 467
AciI CCGC 4 cut(s) 28, 113, 145, 336
AfeI AGCGCT 1 cut(s) 552
AfiI CCNNNNNNNGG 3 cut(s) 347, 467, 659
AflIII ACRYGT 1 cut(s) 685
AjnI CCWGG 3 cut(s) 352, 532, 595
AluBI AGCT 4 cut(s) 281, 302, 434, 610
AluI AGCT 4 cut(s) 281, 302, 434, 610
Alw21I GWGCWC 1 cut(s) 739
Alw26I GTCTC 4 cut(s) 44, 240, 470, 579
Ama87I CYCGRG 2 cut(s) 66, 117
Aor13HI TCCGGA 2 cut(s) 181, 467
Aor51HI AGCGCT 1 cut(s) 552
AoxI GGCC 1 cut(s) 496
ApeKI GCWGC 1 cut(s) 434
AspLEI GCGC 2 cut(s) 217, 553
AspS9I GGNCC 4 cut(s) 333, 350, 530, 650
AsuII TTCGAA 1 cut(s) 262
AvaI CYCGRG 2 cut(s) 66, 117
AvaII GGWCC 4 cut(s) 333, 350, 530, 650
BauI CACGAG 1 cut(s) 108
BbsI GAAGAC 1 cut(s) 380
Bbv12I GWGCWC 1 cut(s) 739
BbvI GCAGC 1 cut(s) 421
BccI CCATC 1 cut(s) 611
BciT130I CCWGG 3 cut(s) 354, 534, 597
BcoDI GTCTC 4 cut(s) 44, 240, 470, 579
BfoI RGCGCY 1 cut(s) 554
BisI GCNGC 2 cut(s) 114, 435
BlsI GCNGC 2 cut(s) 115, 436
Bme1390I CCNGG 3 cut(s) 354, 534, 597
Bme18I GGWCC 4 cut(s) 333, 350, 530, 650
BmeT110I CYCGRG 2 cut(s) 66, 117
BmgT120I GGNCC 4 cut(s) 333, 350, 530, 650
BmrFI CCNGG 3 cut(s) 354, 534, 597
BmsI GCATC 1 cut(s) 387
BpiI GAAGAC 1 cut(s) 380
BpmI CTGGAG 2 cut(s) 618, 626
Bpu14I TTCGAA 1 cut(s) 262
BsaI GGTCTC 1 cut(s) 579
BsaJI CCNNGG 3 cut(s) 307, 329, 725
BsaWI WCCGGW 2 cut(s) 181, 467
BsaXI ACNNNNNCTCC 4 cut(s) 423, 453, 615, 645
Bsc4I CCNNNNNNNGG 3 cut(s) 347, 467, 659
Bse118I RCCGGY 1 cut(s) 10
Bse1I ACTGG 2 cut(s) 341, 609
Bse3DI GCAATG 1 cut(s) 674
BseAI TCCGGA 2 cut(s) 181, 467
BseBI CCWGG 3 cut(s) 354, 534, 597
BseDI CCNNGG 3 cut(s) 307, 329, 725
BseGI GGATG 2 cut(s) 580, 622
BseLI CCNNNNNNNGG 3 cut(s) 347, 467, 659
BseMI GCAATG 1 cut(s) 674
BseNI ACTGG 2 cut(s) 341, 609
BseRI GAGGAG 2 cut(s) 256, 636
BseXI GCAGC 1 cut(s) 421
Bsh1236I CGCG 1 cut(s) 657
Bsh1285I CGRYCG 1 cut(s) 23
BshFI GGCC 1 cut(s) 498
BsiEI CGRYCG 1 cut(s) 23
BsiHKAI GWGCWC 1 cut(s) 739
BsiHKCI CYCGRG 2 cut(s) 66, 117
BsiSI CCGG 3 cut(s) 11, 182, 468
BslI CCNNNNNNNGG 3 cut(s) 347, 467, 659
BsmAI GTCTC 4 cut(s) 44, 240, 470, 579
BsmI GAATGC 1 cut(s) 697
BsnI GGCC 1 cut(s) 498
Bso31I GGTCTC 1 cut(s) 579
BsoBI CYCGRG 2 cut(s) 66, 117
Bsp119I TTCGAA 1 cut(s) 262
Bsp1286I GDGCHC 1 cut(s) 739
Bsp13I TCCGGA 2 cut(s) 181, 467
Bsp143I GATC 3 cut(s) 20, 195, 208
Bsp19I CCATGG 2 cut(s) 307, 725
BspACI CCGC 4 cut(s) 28, 113, 145, 336
BspANI GGCC 1 cut(s) 498
BspEI TCCGGA 2 cut(s) 181, 467
BspFNI CGCG 1 cut(s) 657
BspHI TCATGA 1 cut(s) 237
BspT104I TTCGAA 1 cut(s) 262
BspTNI GGTCTC 1 cut(s) 579
BsrBI CCGCTC 1 cut(s) 113
BsrDI GCAATG 1 cut(s) 674
BsrFI RCCGGY 1 cut(s) 10
BsrI ACTGG 2 cut(s) 341, 609
BssAI RCCGGY 1 cut(s) 10
BssECI CCNNGG 3 cut(s) 307, 329, 725
BssMI GATC 3 cut(s) 20, 195, 208
BssSI CACGAG 1 cut(s) 108
BssT1I CCWWGG 2 cut(s) 307, 725
Bst2BI CACGAG 1 cut(s) 108
Bst2UI CCWGG 3 cut(s) 354, 534, 597
Bst4CI ACNGT 2 cut(s) 98, 450
BstBI TTCGAA 1 cut(s) 262
BstC8I GCNNGC 2 cut(s) 12, 304
BstDEI CTNAG 1 cut(s) 741
BstDSI CCRYGG 3 cut(s) 307, 329, 725
BstF5I GGATG 2 cut(s) 580, 622
BstFNI CGCG 1 cut(s) 657
BstH2I RGCGCY 1 cut(s) 554
BstHHI GCGC 2 cut(s) 217, 553
BstKTI GATC 3 cut(s) 23, 198, 211
BstMAI GTCTC 4 cut(s) 44, 240, 470, 579
BstMBI GATC 3 cut(s) 20, 195, 208
BstMCI CGRYCG 1 cut(s) 23
BstMWI GCNNNNNNNGC 1 cut(s) 223
BstNI CCWGG 3 cut(s) 354, 534, 597
BstNSI RCATGY 2 cut(s) 365, 689
BstSCI CCNGG 3 cut(s) 352, 532, 595
BstUI CGCG 1 cut(s) 657
BstV1I GCAGC 1 cut(s) 421
BstV2I GAAGAC 1 cut(s) 380
BsuRI GGCC 1 cut(s) 498
BtgI CCRYGG 3 cut(s) 307, 329, 725
BtsCI GGATG 2 cut(s) 580, 622
BtsIMutI CAGTG 1 cut(s) 137
Cac8I GCNNGC 2 cut(s) 12, 304
CciI TCATGA 1 cut(s) 237
CfoI GCGC 2 cut(s) 217, 553
Cfr10I RCCGGY 1 cut(s) 10
Cfr13I GGNCC 4 cut(s) 333, 350, 530, 650
CpoI CGGWCCG 1 cut(s) 333
CseI GACGC 2 cut(s) 209, 479
CspI CGGWCCG 1 cut(s) 333
CviAII CATG 5 cut(s) 238, 308, 362, 686, 726
CviJI RGCY 7 cut(s) 14, 116, 281, 302, 434, 498, 610
CviKI_1 RGCY 7 cut(s) 14, 116, 281, 302, 434, 498, 610
DdeI CTNAG 1 cut(s) 741
DpnI GATC 3 cut(s) 22, 197, 210
DpnII GATC 3 cut(s) 20, 195, 208
DraI TTTAAA 1 cut(s) 525
Eco130I CCWWGG 2 cut(s) 307, 725
Eco31I GGTCTC 1 cut(s) 579
Eco47I GGWCC 4 cut(s) 333, 350, 530, 650
Eco47III AGCGCT 1 cut(s) 552
Eco88I CYCGRG 2 cut(s) 66, 117
EcoRII CCWGG 3 cut(s) 352, 532, 595
EcoT14I CCWWGG 2 cut(s) 307, 725
ErhI CCWWGG 2 cut(s) 307, 725
FaeI CATG 5 cut(s) 241, 311, 365, 689, 729
FatI CATG 5 cut(s) 237, 307, 361, 685, 725
FauI CCCGC 2 cut(s) 21, 152
FauNDI CATATG 1 cut(s) 393
Fnu4HI GCNGC 2 cut(s) 114, 435
FokI GGATG 2 cut(s) 567, 629
Fsp4HI GCNGC 2 cut(s) 114, 435
GlaI GCGC 2 cut(s) 216, 552
GluI GCNGC 2 cut(s) 114, 435
GsuI CTGGAG 2 cut(s) 618, 626
HaeII RGCGCY 1 cut(s) 554
HaeIII GGCC 1 cut(s) 498
HapII CCGG 3 cut(s) 11, 182, 468
HgaI GACGC 2 cut(s) 209, 479
HhaI GCGC 2 cut(s) 217, 553
Hin1II CATG 5 cut(s) 241, 311, 365, 689, 729
Hin6I GCGC 2 cut(s) 215, 551
HinP1I GCGC 2 cut(s) 215, 551
HindIII AAGCTT 1 cut(s) 279
HinfI GANTC 5 cut(s) 44, 158, 296, 426, 558
HpaII CCGG 3 cut(s) 11, 182, 468
Hpy188I TCNGA 6 cut(s) 49, 125, 157, 163, 195, 431
Hpy188III TCNNGA 5 cut(s) 38, 182, 238, 468, 614
Hpy99I CGWCG 3 cut(s) 21, 203, 495
HpyAV CCTTC 1 cut(s) 416
HpyCH4III ACNGT 2 cut(s) 98, 450
HpyCH4IV ACGT 1 cut(s) 220
HpyCH4V TGCA 1 cut(s) 716
HpyF10VI GCNNNNNNNGC 1 cut(s) 223
HpyF3I CTNAG 1 cut(s) 741
HpySE526I ACGT 1 cut(s) 220
Hsp92II CATG 5 cut(s) 241, 311, 365, 689, 729
HspAI GCGC 2 cut(s) 215, 551
Kpn2I TCCGGA 2 cut(s) 181, 467
KroI GCCGGC 1 cut(s) 10
KroNI GCCGGC 1 cut(s) 12
Kzo9I GATC 3 cut(s) 20, 195, 208
LmnI GCTCC 4 cut(s) 431, 487, 607, 742
Lsp1109I GCAGC 1 cut(s) 421
LweI GCATC 1 cut(s) 387
MaeII ACGT 1 cut(s) 220
MaeIII GTNAC 2 cut(s) 58, 322
MalI GATC 3 cut(s) 22, 197, 210
MbiI CCGCTC 1 cut(s) 113
MboI GATC 3 cut(s) 20, 195, 208
MboII GAAGA 2 cut(s) 380, 638
MhlI GDGCHC 1 cut(s) 739
MluCI AATT 1 cut(s) 264
MlyI GAGTC 3 cut(s) 167, 435, 552
MmeI TCCRAC 1 cut(s) 409
MnlI CCTC 9 cut(s) 34, 163, 189, 234, 265, 350, 593, 614, 663
MroI TCCGGA 2 cut(s) 181, 467
MroNI GCCGGC 1 cut(s) 10
MseI TTAA 4 cut(s) 33, 173, 524, 753
MslI CAYNNNNRTG 2 cut(s) 366, 690
MspI CCGG 3 cut(s) 11, 182, 468
MspR9I CCNGG 3 cut(s) 354, 534, 597
Mva1269I GAATGC 1 cut(s) 697
MvaI CCWGG 3 cut(s) 354, 534, 597
MvnI CGCG 1 cut(s) 657
MwoI GCNNNNNNNGC 1 cut(s) 223
NaeI GCCGGC 1 cut(s) 12
NcoI CCATGG 2 cut(s) 307, 725
NdeI CATATG 1 cut(s) 393
NdeII GATC 3 cut(s) 20, 195, 208
NgoMIV GCCGGC 1 cut(s) 10
NlaIII CATG 5 cut(s) 241, 311, 365, 689, 729
NspI RCATGY 2 cut(s) 365, 689
NspV TTCGAA 1 cut(s) 262
PagI TCATGA 1 cut(s) 237
PciI ACATGT 1 cut(s) 685
PctI GAATGC 1 cut(s) 697
PdiI GCCGGC 1 cut(s) 12
PfeI GAWTC 2 cut(s) 44, 296
PflMI CCANNNNNTGG 1 cut(s) 347
PfoI TCCNGGA 1 cut(s) 595
PkrI GCNGC 2 cut(s) 115, 436
Ple19I CGATCG 1 cut(s) 23
PleI GAGTC 3 cut(s) 166, 434, 552
PpsI GAGTC 3 cut(s) 166, 434, 552
PscI ACATGT 1 cut(s) 685
Psp6I CCWGG 3 cut(s) 352, 532, 595
PspGI CCWGG 3 cut(s) 352, 532, 595
PspPI GGNCC 4 cut(s) 333, 350, 530, 650
PvuI CGATCG 1 cut(s) 23
RseI CAYNNNNRTG 2 cut(s) 366, 690
Rsr2I CGGWCCG 1 cut(s) 333
RsrII CGGWCCG 1 cut(s) 333
SaqAI TTAA 4 cut(s) 33, 173, 524, 753
SatI GCNGC 2 cut(s) 114, 435
Sau3AI GATC 3 cut(s) 20, 195, 208
Sau96I GGNCC 4 cut(s) 333, 350, 530, 650
SchI GAGTC 3 cut(s) 167, 435, 552
ScrFI CCNGG 3 cut(s) 354, 534, 597
SduI GDGCHC 1 cut(s) 739
SfaNI GCATC 1 cut(s) 387
SfuI TTCGAA 1 cut(s) 262
SinI GGWCC 4 cut(s) 333, 350, 530, 650
SmiMI CAYNNNNRTG 2 cut(s) 366, 690
Sse9I AATT 1 cut(s) 264
SsiI CCGC 4 cut(s) 28, 113, 145, 336
SspI AATATT 1 cut(s) 406
StyD4I CCNGG 3 cut(s) 352, 532, 595
StyI CCWWGG 2 cut(s) 307, 725
TaaI ACNGT 2 cut(s) 98, 450
TaiI ACGT 1 cut(s) 223
TaqI TCGA 4 cut(s) 19, 23, 198, 262
TasI AATT 1 cut(s) 264
TauI GCSGC 1 cut(s) 116
TfiI GAWTC 2 cut(s) 44, 296
Tru1I TTAA 4 cut(s) 33, 173, 524, 753
Tru9I TTAA 4 cut(s) 33, 173, 524, 753
TscAI CASTG 1 cut(s) 144
TseI GCWGC 1 cut(s) 434
TspDTI ATGAA 4 cut(s) 17, 182, 226, 380
TspGWI ACGGA 2 cut(s) 94, 346
TspRI CASTG 1 cut(s) 144
Van91I CCANNNNNTGG 1 cut(s) 347
VpaK11BI GGWCC 4 cut(s) 333, 350, 530, 650
XceI RCATGY 2 cut(s) 365, 689
XcmI CCANNNNNNNNNTGG 1 cut(s) 314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.