MD14G1054600.v1.1

DNA-3-methyladenine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0008717
Physical Location & Seq
Forward (+)
5512391 .. 5515238
2848 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1054600.v1.1.491

Sequence Viewer

Length: 834 bp
ATGAAGAAACCTCGGGCATTTAAACGAGTCGCCGCCAAACCCAAGAGGCCAATTCCATCCAACCGTCTCACTAATGAAGAAGACAAAACTCGGCCGATTCAATGTTCGGAATCGTTGACCGTCGCAGCGGTCAAACCCAAAAAACCCCAATCTCAACCTCAAACCAAATCCGACTCATCACTCCCAATCCCAGACTTTCCTCCCGAGAAAATGACCATCTTGCCCTTCTCATTCTTCCAAATCGACGCCCTAGAGCTCGCCCCACGTTTGCTGGGCAAGTTTCTGAGGCGAGAGGATGTTGTTCTTCAGATTACTGAGGTAGAAGCTTATAGGCCAAATGATTCGGTTTGTCATGGTCGATTTGGCATAACTGCAAGAACAGCCCCCGTTTTTGGACCTGGAGGGCATGCGTATGTTTATCTTTGCTACGGCCTCCATACAATGCTGAATGTTGTTGCTGACAAGGAGGGGGTTGGGGCTGCTGTCCTAATACGTTCTTGCGCTCCAGTTAGTGAAATAATGAAAGTCATTACAGGATTGGAGACCATTCAGCAGCGTCGAGGTCAGAAAACTGATAAACCCGTGCTCCTTAATGGACCTGGAAAGATTGGTCAAGCGCTGGGACTTTCTACCGAGTGGTCAAGCCATCCCCTTTATACTCCAGATGGTCTGGAGATCTTAGATGGTCCGGAGCCCGAAAAGATGCTGGTTGGTCCGCGTGTGGGAATTGATTACGCTTCACCTGAGCATGTCAATGCATTGTGGAGATTTGCAGTTGCAGGTACCTCTTGGATTAGTGCTCCTAAAAACACCCTCAGGCCACCTGAATCTTAA

Protein Analysis

278

Amino Acids

30.41

Weight (kDa)

9.31

Isoelectric Point (pI)

48.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pur_DNA_glyco PF02245 77 - 262 1.1e-49 Methylpurine-DNA glycosylase (MPG)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 770
Acc65I GGTACC 1 cut(s) 782
AccB1I GGYRCC 1 cut(s) 782
AccII CGCG 1 cut(s) 718
AccIII TCCGGA 1 cut(s) 688
AciI CCGC 3 cut(s) 33, 128, 716
AcoI YGGCCR 1 cut(s) 92
AcuI CTGAAG 1 cut(s) 290
AcyI GRCGYC 1 cut(s) 246
AfaI GTAC 1 cut(s) 784
AfeI AGCGCT 1 cut(s) 618
AfiI CCNNNNNNNGG 2 cut(s) 392, 722
AgsI TTSAA 1 cut(s) 101
AjnI CCWGG 2 cut(s) 397, 598
AluBI AGCT 2 cut(s) 256, 326
AluI AGCT 2 cut(s) 256, 326
Alw21I GWGCWC 3 cut(s) 258, 588, 802
Alw26I GTCTC 2 cut(s) 71, 536
Ama87I CYCGRG 2 cut(s) 12, 203
Aor13HI TCCGGA 1 cut(s) 688
Aor51HI AGCGCT 1 cut(s) 618
AoxI GGCC 5 cut(s) 47, 92, 332, 430, 818
ApeKI GCWGC 3 cut(s) 125, 479, 553
Asp718I GGTACC 1 cut(s) 782
AspLEI GCGC 2 cut(s) 503, 619
AspS9I GGNCC 4 cut(s) 395, 596, 686, 713
AsuHPI GGTGA 1 cut(s) 732
AvaI CYCGRG 2 cut(s) 12, 203
AvaII GGWCC 4 cut(s) 395, 596, 686, 713
AxyI CCTNAGG 1 cut(s) 815
BanI GGYRCC 1 cut(s) 782
BanII GRGCYC 2 cut(s) 258, 696
BbsI GAAGAC 1 cut(s) 87
Bbv12I GWGCWC 3 cut(s) 258, 588, 802
BbvI GCAGC 3 cut(s) 137, 466, 565
BccI CCATC 5 cut(s) 64, 224, 654, 659, 677
BceAI ACGGC 1 cut(s) 445
BciT130I CCWGG 2 cut(s) 399, 600
BcoDI GTCTC 2 cut(s) 71, 536
BfaI CTAG 1 cut(s) 251
BfoI RGCGCY 1 cut(s) 620
BfuAI ACCTGC 1 cut(s) 770
BglII AGATCT 1 cut(s) 675
BisI GCNGC 4 cut(s) 33, 126, 480, 554
BlsI GCNGC 4 cut(s) 34, 127, 481, 555
Bme1390I CCNGG 2 cut(s) 399, 600
Bme18I GGWCC 4 cut(s) 395, 596, 686, 713
BmeT110I CYCGRG 2 cut(s) 12, 203
BmgT120I GGNCC 4 cut(s) 395, 596, 686, 713
BmiI GGNNCC 2 cut(s) 693, 784
BmrFI CCNGG 2 cut(s) 399, 600
BmsI GCATC 1 cut(s) 693
BpiI GAAGAC 1 cut(s) 87
BpmI CTGGAG 4 cut(s) 420, 489, 645, 692
Bpu10I CCTNAGC 1 cut(s) 744
BsaHI GRCGYC 1 cut(s) 246
BsaI GGTCTC 1 cut(s) 536
BsaJI CCNNGG 1 cut(s) 11
BsaWI WCCGGW 1 cut(s) 688
BsaXI ACNNNNNCTCC 2 cut(s) 570, 600
Bsc4I CCNNNNNNNGG 2 cut(s) 392, 722
Bse1I ACTGG 1 cut(s) 506
Bse21I CCTNAGG 1 cut(s) 815
BseAI TCCGGA 1 cut(s) 688
BseBI CCWGG 2 cut(s) 399, 600
BseDI CCNNGG 1 cut(s) 11
BseGI GGATG 3 cut(s) 56, 301, 646
BseLI CCNNNNNNNGG 2 cut(s) 392, 722
BseMII CTCAG 4 cut(s) 275, 306, 735, 829
BseNI ACTGG 1 cut(s) 506
BseX3I CGGCCG 1 cut(s) 92
BseXI GCAGC 3 cut(s) 137, 466, 565
BseYI CCCAGC 2 cut(s) 271, 619
Bsh1236I CGCG 1 cut(s) 718
Bsh1285I CGRYCG 1 cut(s) 95
BshFI GGCC 5 cut(s) 49, 94, 334, 432, 820
BshNI GGYRCC 1 cut(s) 782
BsiEI CGRYCG 1 cut(s) 95
BsiHKAI GWGCWC 3 cut(s) 258, 588, 802
BsiHKCI CYCGRG 2 cut(s) 12, 203
BsiSI CCGG 1 cut(s) 689
BslFI GGGAC 1 cut(s) 636
BslI CCNNNNNNNGG 2 cut(s) 392, 722
BsmAI GTCTC 2 cut(s) 71, 536
BsmBI CGTCTC 1 cut(s) 71
BsmFI GGGAC 1 cut(s) 636
BsnI GGCC 5 cut(s) 49, 94, 334, 432, 820
Bso31I GGTCTC 1 cut(s) 536
BsoBI CYCGRG 2 cut(s) 12, 203
Bsp1286I GDGCHC 4 cut(s) 258, 588, 696, 802
Bsp13I TCCGGA 1 cut(s) 688
Bsp143I GATC 1 cut(s) 675
BspACI CCGC 3 cut(s) 33, 128, 716
BspANI GGCC 5 cut(s) 49, 94, 334, 432, 820
BspCNI CTCAG 4 cut(s) 276, 307, 736, 828
BspEI TCCGGA 1 cut(s) 688
BspFNI CGCG 1 cut(s) 718
BspLI GGNNCC 2 cut(s) 693, 784
BspMI ACCTGC 1 cut(s) 770
BspT107I GGYRCC 1 cut(s) 782
BspTNI GGTCTC 1 cut(s) 536
BsrI ACTGG 1 cut(s) 506
BssECI CCNNGG 1 cut(s) 11
BssMI GATC 1 cut(s) 675
BssNI GRCGYC 1 cut(s) 246
Bst2UI CCWGG 2 cut(s) 399, 600
Bst4CI ACNGT 2 cut(s) 65, 121
BstACI GRCGYC 1 cut(s) 246
BstC8I GCNNGC 2 cut(s) 258, 408
BstDEI CTNAG 5 cut(s) 284, 315, 679, 744, 815
BstF5I GGATG 3 cut(s) 56, 301, 646
BstFNI CGCG 1 cut(s) 718
BstH2I RGCGCY 1 cut(s) 620
BstHHI GCGC 2 cut(s) 503, 619
BstKTI GATC 1 cut(s) 678
BstMAI GTCTC 2 cut(s) 71, 536
BstMBI GATC 1 cut(s) 675
BstMCI CGRYCG 1 cut(s) 95
BstMWI GCNNNNNNNGC 1 cut(s) 380
BstNI CCWGG 2 cut(s) 399, 600
BstNSI RCATGY 2 cut(s) 410, 752
BstSCI CCNGG 2 cut(s) 397, 598
BstUI CGCG 1 cut(s) 718
BstV1I GCAGC 3 cut(s) 137, 466, 565
BstV2I GAAGAC 1 cut(s) 87
BstX2I RGATCY 1 cut(s) 675
BstYI RGATCY 1 cut(s) 675
BstZI CGGCCG 1 cut(s) 92
Bsu36I CCTNAGG 1 cut(s) 815
BsuRI GGCC 5 cut(s) 49, 94, 334, 432, 820
BtsCI GGATG 3 cut(s) 56, 301, 646
BveI ACCTGC 1 cut(s) 770
Cac8I GCNNGC 2 cut(s) 258, 408
CfoI GCGC 2 cut(s) 503, 619
Cfr13I GGNCC 4 cut(s) 395, 596, 686, 713
CseI GACGC 2 cut(s) 254, 545
Csp6I GTAC 1 cut(s) 783
CviAII CATG 3 cut(s) 353, 407, 749
CviQI GTAC 1 cut(s) 783
DdeI CTNAG 5 cut(s) 284, 315, 679, 744, 815
DpnI GATC 1 cut(s) 677
DpnII GATC 1 cut(s) 675
DraI TTTAAA 1 cut(s) 22
EaeI YGGCCR 1 cut(s) 92
EagI CGGCCG 1 cut(s) 92
Ecl136II GAGCTC 1 cut(s) 256
EclXI CGGCCG 1 cut(s) 92
Eco24I GRGCYC 2 cut(s) 258, 696
Eco31I GGTCTC 1 cut(s) 536
Eco47I GGWCC 4 cut(s) 395, 596, 686, 713
Eco47III AGCGCT 1 cut(s) 618
Eco52I CGGCCG 1 cut(s) 92
Eco53kI GAGCTC 1 cut(s) 256
Eco57I CTGAAG 1 cut(s) 290
Eco81I CCTNAGG 1 cut(s) 815
Eco88I CYCGRG 2 cut(s) 12, 203
EcoICRI GAGCTC 1 cut(s) 256
EcoRII CCWGG 2 cut(s) 397, 598
EcoT22I ATGCAT 1 cut(s) 760
EcoT38I GRGCYC 2 cut(s) 258, 696
Esp3I CGTCTC 1 cut(s) 71
FaeI CATG 3 cut(s) 356, 410, 752
FaiI YATR 8 cut(s) 330, 354, 368, 408, 414, 438, 657, 750
FaqI GGGAC 1 cut(s) 636
FatI CATG 3 cut(s) 352, 406, 748
Fnu4HI GCNGC 4 cut(s) 33, 126, 480, 554
FokI GGATG 3 cut(s) 43, 308, 633
FriOI GRGCYC 2 cut(s) 258, 696
Fsp4HI GCNGC 4 cut(s) 33, 126, 480, 554
FspBI CTAG 1 cut(s) 251
GlaI GCGC 2 cut(s) 502, 618
GluI GCNGC 4 cut(s) 33, 126, 480, 554
GsaI CCCAGC 2 cut(s) 275, 623
GsuI CTGGAG 4 cut(s) 420, 489, 645, 692
HaeII RGCGCY 1 cut(s) 620
HaeIII GGCC 5 cut(s) 49, 94, 334, 432, 820
HapII CCGG 1 cut(s) 689
HgaI GACGC 2 cut(s) 254, 545
HhaI GCGC 2 cut(s) 503, 619
Hin1I GRCGYC 1 cut(s) 246
Hin1II CATG 3 cut(s) 356, 410, 752
Hin6I GCGC 2 cut(s) 501, 617
HinP1I GCGC 2 cut(s) 501, 617
HincII GTYRAC 1 cut(s) 117
HindII GTYRAC 1 cut(s) 117
HindIII AAGCTT 1 cut(s) 324
HinfI GANTC 6 cut(s) 27, 97, 110, 173, 341, 827
HpaII CCGG 1 cut(s) 689
HphI GGTGA 1 cut(s) 732
Hpy166II GTNNAC 1 cut(s) 117
Hpy188I TCNGA 5 cut(s) 109, 172, 285, 309, 567
Hpy188III TCNNGA 4 cut(s) 203, 662, 671, 689
Hpy8I GTNNAC 1 cut(s) 117
Hpy99I CGWCG 3 cut(s) 125, 248, 561
HpyAV CCTTC 1 cut(s) 235
HpyCH4III ACNGT 2 cut(s) 65, 121
HpyCH4IV ACGT 2 cut(s) 265, 493
HpyCH4V TGCA 4 cut(s) 374, 758, 773, 779
HpyF10VI GCNNNNNNNGC 1 cut(s) 380
HpyF3I CTNAG 5 cut(s) 284, 315, 679, 744, 815
HpySE526I ACGT 2 cut(s) 265, 493
Hsp92I GRCGYC 1 cut(s) 246
Hsp92II CATG 3 cut(s) 356, 410, 752
HspAI GCGC 2 cut(s) 501, 617
Kpn2I TCCGGA 1 cut(s) 688
KpnI GGTACC 1 cut(s) 786
Kzo9I GATC 1 cut(s) 675
LmnI GCTCC 4 cut(s) 508, 591, 691, 805
Lsp1109I GCAGC 3 cut(s) 137, 466, 565
LweI GCATC 1 cut(s) 693
MaeI CTAG 1 cut(s) 251
MaeII ACGT 2 cut(s) 265, 493
MalI GATC 1 cut(s) 677
MboI GATC 1 cut(s) 675
MboII GAAGA 5 cut(s) 16, 89, 92, 226, 296
MflI RGATCY 1 cut(s) 675
MhlI GDGCHC 4 cut(s) 258, 588, 696, 802
MluCI AATT 2 cut(s) 51, 726
MlyI GAGTC 2 cut(s) 36, 167
MmeI TCCRAC 2 cut(s) 84, 195
Mph1103I ATGCAT 1 cut(s) 760
MroI TCCGGA 1 cut(s) 688
MseI TTAA 3 cut(s) 21, 591, 832
MslI CAYNNNNRTG 2 cut(s) 411, 753
MspA1I CMGCKG 1 cut(s) 128
MspI CCGG 1 cut(s) 689
MspR9I CCNGG 2 cut(s) 399, 600
MvaI CCWGG 2 cut(s) 399, 600
MvnI CGCG 1 cut(s) 718
MwoI GCNNNNNNNGC 1 cut(s) 380
NdeII GATC 1 cut(s) 675
NlaIII CATG 3 cut(s) 356, 410, 752
NlaIV GGNNCC 2 cut(s) 693, 784
NmeAIII GCCGAG 1 cut(s) 70
NsiI ATGCAT 1 cut(s) 760
NspI RCATGY 2 cut(s) 410, 752
PaeI GCATGC 1 cut(s) 410
PfeI GAWTC 4 cut(s) 97, 110, 341, 827
PkrI GCNGC 4 cut(s) 34, 127, 481, 555
PleI GAGTC 2 cut(s) 35, 167
PpsI GAGTC 2 cut(s) 35, 167
Psp124BI GAGCTC 1 cut(s) 258
Psp6I CCWGG 2 cut(s) 397, 598
PspFI CCCAGC 2 cut(s) 271, 619
PspGI CCWGG 2 cut(s) 397, 598
PspN4I GGNNCC 2 cut(s) 693, 784
PspPI GGNCC 4 cut(s) 395, 596, 686, 713
PsuI RGATCY 1 cut(s) 675
RsaI GTAC 1 cut(s) 784
RsaNI GTAC 1 cut(s) 783
RseI CAYNNNNRTG 2 cut(s) 411, 753
SacI GAGCTC 1 cut(s) 258
SaqAI TTAA 3 cut(s) 21, 591, 832
SatI GCNGC 4 cut(s) 33, 126, 480, 554
Sau3AI GATC 1 cut(s) 675
Sau96I GGNCC 4 cut(s) 395, 596, 686, 713
SchI GAGTC 2 cut(s) 36, 167
ScrFI CCNGG 2 cut(s) 399, 600
SduI GDGCHC 4 cut(s) 258, 588, 696, 802
SfaNI GCATC 1 cut(s) 693
SinI GGWCC 4 cut(s) 395, 596, 686, 713
SmiMI CAYNNNNRTG 2 cut(s) 411, 753
SphI GCATGC 1 cut(s) 410
Sse9I AATT 2 cut(s) 51, 726
SsiI CCGC 3 cut(s) 33, 128, 716
SspMI CTAG 1 cut(s) 251
SstI GAGCTC 1 cut(s) 258
StyD4I CCNGG 2 cut(s) 397, 598
TaaI ACNGT 2 cut(s) 65, 121
TaiI ACGT 2 cut(s) 268, 496
TaqI TCGA 3 cut(s) 243, 358, 559
TasI AATT 2 cut(s) 51, 726
TauI GCSGC 1 cut(s) 35
TfiI GAWTC 4 cut(s) 97, 110, 341, 827
Tru1I TTAA 3 cut(s) 21, 591, 832
Tru9I TTAA 3 cut(s) 21, 591, 832
TseI GCWGC 3 cut(s) 125, 479, 553
TspDTI ATGAA 3 cut(s) 17, 90, 536
VpaK11BI GGWCC 4 cut(s) 395, 596, 686, 713
XceI RCATGY 2 cut(s) 410, 752
XspI CTAG 1 cut(s) 251
Zsp2I ATGCAT 1 cut(s) 760
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.