Rh3BG257600

DNA-3-methyladenine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0008717
Physical Location & Seq
Forward (+)
24924267 .. 24926739
2473 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG257600.1

Sequence Viewer

Length: 714 bp
ATGAAGAGAGCTCCGCGATTCAAACGAGTCGCCGCTAAAACCCCCAAGCGGCCAACACTCTCAAACCGACCCAGCGATGACGACGACGACCAAACTCCGCCGAGTCAACCCCAACCCGACTCAACTCCAATCCCAGAGATTCCTGCGGATAAAATGACCATCTTGCCCTACACATTCTTCCAGATTGACGCCCTAGACCTCGCCCCGCGTTTGCTCGGCAAGTTCCTTAGGAGAGACGACGTTGTTCTTCAGATTACCGAGGTAGAAGCTTACAGACCAAATGATTCGGCTTGTCACGGTCGATTTGGCATAACAACAAGAACGGCCCCTGTTTTTGGACCAGGAGGGCATGCATATGTTTATCTTTGCTATGGTCTTCATTCGATGCTGAATGTTGTTGCTGACAAGGAGGGAGTTGGGGCGGCTGTCCTAATACGTGCTTGCGCTCCAATTAGTGGATTGGAGACCATACAGCAACGTCGAGGGCAGAAGACTAGTAAGCCTGTTCTCCTTAGTGGACCAGGAAAGATGACTGTCTGGCCAGGTGGTCTGGAAATCTTAGATGGTCCCCAACCTGAAAACATGTTGATTGGTCCGCGTGTAGGCATCAACTATGCTTTGCCTGAGCACGTCAATGCGTTGTGGAGATTTGCAATTGCAGGTACCCCTTGGATAAGTGCTCCGAAGAACACTCTCAGGCCACCGTCATCTTAA

Protein Analysis

237

Amino Acids

25.9

Weight (kDa)

9.32

Isoelectric Point (pI)

52.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pur_DNA_glyco PF02245 59 - 222 1.6e-42 Methylpurine-DNA glycosylase (MPG)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 650
Acc65I GGTACC 1 cut(s) 662
AccB1I GGYRCC 1 cut(s) 662
AccB7I CCANNNNNTGG 1 cut(s) 455
AccII CGCG 3 cut(s) 16, 208, 598
AciI CCGC 8 cut(s) 14, 33, 49, 98, 146, 206, 422, 596
AcoI YGGCCR 2 cut(s) 50, 539
AcuI CTGAAG 1 cut(s) 233
AcyI GRCGYC 1 cut(s) 189
AfaI GTAC 1 cut(s) 664
AfiI CCNNNNNNNGG 4 cut(s) 48, 335, 455, 602
AflIII ACRYGT 1 cut(s) 582
AgsI TTSAA 1 cut(s) 22
AhlI ACTAGT 1 cut(s) 494
AjiI CACGTC 1 cut(s) 631
AjnI CCWGG 3 cut(s) 340, 520, 541
AluBI AGCT 2 cut(s) 11, 269
AluI AGCT 2 cut(s) 11, 269
Alw21I GWGCWC 3 cut(s) 13, 630, 682
Alw26I GTCTC 2 cut(s) 228, 458
AoxI GGCC 4 cut(s) 50, 324, 539, 698
Asp718I GGTACC 1 cut(s) 662
AspLEI GCGC 1 cut(s) 446
AspS9I GGNCC 5 cut(s) 325, 338, 518, 566, 593
AvaII GGWCC 4 cut(s) 338, 518, 566, 593
AxyI CCTNAGG 1 cut(s) 227
BalI TGGCCA 1 cut(s) 541
BanI GGYRCC 1 cut(s) 662
BanII GRGCYC 1 cut(s) 13
BbsI GAAGAC 2 cut(s) 368, 497
Bbv12I GWGCWC 3 cut(s) 13, 630, 682
BccI CCATC 2 cut(s) 167, 557
BceAI ACGGC 1 cut(s) 339
BciT130I CCWGG 3 cut(s) 342, 522, 543
BcoDI GTCTC 2 cut(s) 228, 458
BcuI ACTAGT 1 cut(s) 494
BfaI CTAG 2 cut(s) 194, 495
BfuAI ACCTGC 1 cut(s) 650
BisI GCNGC 3 cut(s) 33, 50, 423
BlsI GCNGC 3 cut(s) 34, 51, 424
Bme1390I CCNGG 3 cut(s) 342, 522, 543
Bme18I GGWCC 4 cut(s) 338, 518, 566, 593
BmgBI CACGTC 1 cut(s) 631
BmgT120I GGNCC 5 cut(s) 325, 338, 518, 566, 593
BmiI GGNNCC 3 cut(s) 327, 568, 664
BmrFI CCNGG 3 cut(s) 342, 522, 543
BmsI GCATC 2 cut(s) 375, 615
BpiI GAAGAC 2 cut(s) 368, 497
Bpu10I CCTNAGC 1 cut(s) 624
BsaAI YACGTR 1 cut(s) 437
BsaHI GRCGYC 1 cut(s) 189
BsaI GGTCTC 1 cut(s) 458
BsaJI CCNNGG 2 cut(s) 258, 668
Bsc4I CCNNNNNNNGG 4 cut(s) 48, 335, 455, 602
Bse21I CCTNAGG 1 cut(s) 227
BseBI CCWGG 3 cut(s) 342, 522, 543
BseDI CCNNGG 2 cut(s) 258, 668
BseLI CCNNNNNNNGG 4 cut(s) 48, 335, 455, 602
BseMII CTCAG 2 cut(s) 615, 709
BseYI CCCAGC 1 cut(s) 71
Bsh1236I CGCG 3 cut(s) 16, 208, 598
Bsh1285I CGRYCG 1 cut(s) 301
BshFI GGCC 4 cut(s) 52, 326, 541, 700
BshNI GGYRCC 1 cut(s) 662
BsiEI CGRYCG 1 cut(s) 301
BsiHKAI GWGCWC 3 cut(s) 13, 630, 682
BslFI GGGAC 1 cut(s) 552
BslI CCNNNNNNNGG 4 cut(s) 48, 335, 455, 602
BsmAI GTCTC 2 cut(s) 228, 458
BsmBI CGTCTC 1 cut(s) 228
BsmFI GGGAC 1 cut(s) 552
BsnI GGCC 4 cut(s) 52, 326, 541, 700
Bso31I GGTCTC 1 cut(s) 458
Bsp1286I GDGCHC 3 cut(s) 13, 630, 682
BspACI CCGC 8 cut(s) 14, 33, 49, 98, 146, 206, 422, 596
BspANI GGCC 4 cut(s) 52, 326, 541, 700
BspCNI CTCAG 2 cut(s) 616, 708
BspFNI CGCG 3 cut(s) 16, 208, 598
BspLI GGNNCC 3 cut(s) 327, 568, 664
BspMI ACCTGC 1 cut(s) 650
BspT107I GGYRCC 1 cut(s) 662
BspTNI GGTCTC 1 cut(s) 458
BssECI CCNNGG 2 cut(s) 258, 668
BssNI GRCGYC 1 cut(s) 189
BssT1I CCWWGG 1 cut(s) 668
Bst2UI CCWGG 3 cut(s) 342, 522, 543
Bst4CI ACNGT 3 cut(s) 299, 535, 705
BstACI GRCGYC 1 cut(s) 189
BstBAI YACGTR 1 cut(s) 437
BstC8I GCNNGC 2 cut(s) 351, 442
BstDEI CTNAG 5 cut(s) 227, 512, 559, 624, 695
BstFNI CGCG 3 cut(s) 16, 208, 598
BstHHI GCGC 1 cut(s) 446
BstMAI GTCTC 2 cut(s) 228, 458
BstMCI CGRYCG 1 cut(s) 301
BstNI CCWGG 3 cut(s) 342, 522, 543
BstNSI RCATGY 2 cut(s) 353, 586
BstSCI CCNGG 3 cut(s) 340, 520, 541
BstUI CGCG 3 cut(s) 16, 208, 598
BstV2I GAAGAC 2 cut(s) 368, 497
Bsu36I CCTNAGG 1 cut(s) 227
BsuRI GGCC 4 cut(s) 52, 326, 541, 700
BtgZI GCGATG 1 cut(s) 90
BtrI CACGTC 1 cut(s) 631
BveI ACCTGC 1 cut(s) 650
Cac8I GCNNGC 2 cut(s) 351, 442
CfoI GCGC 1 cut(s) 446
Cfr13I GGNCC 5 cut(s) 325, 338, 518, 566, 593
CseI GACGC 1 cut(s) 197
Csp6I GTAC 1 cut(s) 663
CviAII CATG 2 cut(s) 350, 583
CviJI RGCY 9 cut(s) 11, 52, 269, 290, 326, 425, 502, 541, 700
CviKI_1 RGCY 9 cut(s) 11, 52, 269, 290, 326, 425, 502, 541, 700
CviQI GTAC 1 cut(s) 663
DdeI CTNAG 5 cut(s) 227, 512, 559, 624, 695
EaeI YGGCCR 2 cut(s) 50, 539
EciI GGCGGA 1 cut(s) 87
Ecl136II GAGCTC 1 cut(s) 11
Eco130I CCWWGG 1 cut(s) 668
Eco24I GRGCYC 1 cut(s) 13
Eco31I GGTCTC 1 cut(s) 458
Eco47I GGWCC 4 cut(s) 338, 518, 566, 593
Eco53kI GAGCTC 1 cut(s) 11
Eco57I CTGAAG 1 cut(s) 233
Eco81I CCTNAGG 1 cut(s) 227
EcoICRI GAGCTC 1 cut(s) 11
EcoRII CCWGG 3 cut(s) 340, 520, 541
EcoT14I CCWWGG 1 cut(s) 668
EcoT22I ATGCAT 1 cut(s) 355
EcoT38I GRGCYC 1 cut(s) 13
ErhI CCWWGG 1 cut(s) 668
Esp3I CGTCTC 1 cut(s) 228
FaeI CATG 2 cut(s) 353, 586
FaiI YATR 8 cut(s) 311, 351, 355, 357, 372, 470, 584, 615
FaqI GGGAC 1 cut(s) 552
FatI CATG 2 cut(s) 349, 582
FauI CCCGC 1 cut(s) 213
FauNDI CATATG 1 cut(s) 355
Fnu4HI GCNGC 3 cut(s) 33, 50, 423
FriOI GRGCYC 1 cut(s) 13
Fsp4HI GCNGC 3 cut(s) 33, 50, 423
FspBI CTAG 2 cut(s) 194, 495
GlaI GCGC 1 cut(s) 445
GluI GCNGC 3 cut(s) 33, 50, 423
GsaI CCCAGC 1 cut(s) 75
HaeIII GGCC 4 cut(s) 52, 326, 541, 700
HgaI GACGC 1 cut(s) 197
HhaI GCGC 1 cut(s) 446
Hin1I GRCGYC 1 cut(s) 189
Hin1II CATG 2 cut(s) 353, 586
Hin6I GCGC 1 cut(s) 444
HinP1I GCGC 1 cut(s) 444
HincII GTYRAC 1 cut(s) 107
HindII GTYRAC 1 cut(s) 107
HindIII AAGCTT 1 cut(s) 267
HinfI GANTC 6 cut(s) 18, 27, 103, 119, 139, 284
Hpy166II GTNNAC 2 cut(s) 107, 518
Hpy188I TCNGA 2 cut(s) 252, 684
Hpy188III TCNNGA 2 cut(s) 181, 551
Hpy8I GTNNAC 2 cut(s) 107, 518
Hpy99I CGWCG 4 cut(s) 86, 89, 242, 483
HpyCH4III ACNGT 3 cut(s) 299, 535, 705
HpyCH4IV ACGT 4 cut(s) 240, 436, 478, 630
HpyCH4V TGCA 3 cut(s) 353, 653, 659
HpyF3I CTNAG 5 cut(s) 227, 512, 559, 624, 695
HpySE526I ACGT 4 cut(s) 240, 436, 478, 630
Hsp92I GRCGYC 1 cut(s) 189
Hsp92II CATG 2 cut(s) 353, 586
HspAI GCGC 1 cut(s) 444
KpnI GGTACC 1 cut(s) 666
LmnI GCTCC 3 cut(s) 16, 451, 685
LweI GCATC 2 cut(s) 375, 615
MaeI CTAG 2 cut(s) 194, 495
MaeII ACGT 4 cut(s) 240, 436, 478, 630
MaeIII GTNAC 1 cut(s) 293
MboII GAAGA 6 cut(s) 16, 169, 239, 368, 502, 697
MfeI CAATTG 1 cut(s) 654
MhlI GDGCHC 3 cut(s) 13, 630, 682
MlsI TGGCCA 1 cut(s) 541
MluCI AATT 2 cut(s) 450, 654
MluNI TGGCCA 1 cut(s) 541
MlyI GAGTC 3 cut(s) 36, 112, 113
MnlI CCTC 5 cut(s) 209, 253, 338, 403, 476
Mox20I TGGCCA 1 cut(s) 541
Mph1103I ATGCAT 1 cut(s) 355
MscI TGGCCA 1 cut(s) 541
MseI TTAA 1 cut(s) 712
MslI CAYNNNNRTG 2 cut(s) 354, 633
Msp20I TGGCCA 1 cut(s) 541
MspR9I CCNGG 3 cut(s) 342, 522, 543
MunI CAATTG 1 cut(s) 654
MvaI CCWGG 3 cut(s) 342, 522, 543
MvnI CGCG 3 cut(s) 16, 208, 598
NdeI CATATG 1 cut(s) 355
NlaIII CATG 2 cut(s) 353, 586
NlaIV GGNNCC 3 cut(s) 327, 568, 664
NmeAIII GCCGAG 2 cut(s) 126, 195
NmuCI GTSAC 1 cut(s) 293
NsiI ATGCAT 1 cut(s) 355
NspI RCATGY 2 cut(s) 353, 586
PaeI GCATGC 1 cut(s) 353
PciI ACATGT 1 cut(s) 582
PfeI GAWTC 3 cut(s) 18, 139, 284
PflMI CCANNNNNTGG 1 cut(s) 455
PkrI GCNGC 3 cut(s) 34, 51, 424
PleI GAGTC 3 cut(s) 35, 111, 113
PpsI GAGTC 3 cut(s) 35, 111, 113
Ppu21I YACGTR 1 cut(s) 437
PscI ACATGT 1 cut(s) 582
Psp124BI GAGCTC 1 cut(s) 13
Psp6I CCWGG 3 cut(s) 340, 520, 541
PspFI CCCAGC 1 cut(s) 71
PspGI CCWGG 3 cut(s) 340, 520, 541
PspN4I GGNNCC 3 cut(s) 327, 568, 664
PspPI GGNCC 5 cut(s) 325, 338, 518, 566, 593
RsaI GTAC 1 cut(s) 664
RsaNI GTAC 1 cut(s) 663
RseI CAYNNNNRTG 2 cut(s) 354, 633
SacI GAGCTC 1 cut(s) 13
SaqAI TTAA 1 cut(s) 712
SatI GCNGC 3 cut(s) 33, 50, 423
Sau96I GGNCC 5 cut(s) 325, 338, 518, 566, 593
SchI GAGTC 3 cut(s) 36, 112, 113
ScrFI CCNGG 3 cut(s) 342, 522, 543
SduI GDGCHC 3 cut(s) 13, 630, 682
SfaNI GCATC 2 cut(s) 375, 615
SinI GGWCC 4 cut(s) 338, 518, 566, 593
SmiMI CAYNNNNRTG 2 cut(s) 354, 633
SpeI ACTAGT 1 cut(s) 494
SphI GCATGC 1 cut(s) 353
Sse9I AATT 2 cut(s) 450, 654
SsiI CCGC 8 cut(s) 14, 33, 49, 98, 146, 206, 422, 596
SspMI CTAG 2 cut(s) 194, 495
SstI GAGCTC 1 cut(s) 13
StyD4I CCNGG 3 cut(s) 340, 520, 541
StyI CCWWGG 1 cut(s) 668
TaaI ACNGT 3 cut(s) 299, 535, 705
TaiI ACGT 4 cut(s) 243, 439, 481, 633
TaqI TCGA 3 cut(s) 301, 383, 481
TasI AATT 2 cut(s) 450, 654
TauI GCSGC 3 cut(s) 35, 52, 425
TfiI GAWTC 3 cut(s) 18, 139, 284
Tru1I TTAA 1 cut(s) 712
Tru9I TTAA 1 cut(s) 712
TseFI GTSAC 1 cut(s) 293
Tsp45I GTSAC 1 cut(s) 293
TspDTI ATGAA 2 cut(s) 17, 368
Van91I CCANNNNNTGG 1 cut(s) 455
VpaK11BI GGWCC 4 cut(s) 338, 518, 566, 593
XceI RCATGY 2 cut(s) 353, 586
XspI CTAG 2 cut(s) 194, 495
Zsp2I ATGCAT 1 cut(s) 355
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.