RLG00000023587

DNA-3-methyladenine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0008717
Physical Location & Seq
Reverse (-)
25651255 .. 25653794
2540 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023587

Sequence Viewer

Length: 789 bp
ATGAAGAGAGCTCCGCGATTCAAACGAATCGCCACTAAATCCAAGCGCGCAATACTCTCAAACCCACTCAGGGATGACGACGGCGACGACCAAACTCCGCCGAGTCAACCTTCCCAGTCGTTGACAGCAGCGGTGGTCAAACCCCAACCCGACTCAACTCCAATCCCAGAGATTCCTCCCGATAAAATGACCATCTTGCCCTACACATTCTTCCAGATTGACGCCCTAGACCTCGCCCCGCGTTTGCTCGGCAAGTTCCTTAGGAGAGACGACGTTGTTCTTCAGATTACCGAGGTTGAAGCTTACAGACCAAATGATTCCGCTTGTCATGGTCGATTTGGCATAACACCAAGAACAGCCCCTGTTTTTGGACCAGGAGGGCGTGCTTATGTTTATCTTTGCTATGGTCTTCATTCAATGCTGAATGTTGTTGCTGACAAGGAGGGAGTCGGGGCGGCTGTCCTAATACGTGCTTGCGCTCCAATTAGTGGATTGGAGACCATACAGCAACGTCGAGGGCAGAAGACTAGTAAGCCTGTTCTCCTTAGTGGACCAGGAAAGATCGGACAAGCCCTGGGACTTTCTACAGAATGGTCTAACCACCCCCTCTACACTCCTGGTGGTCTTGAAATCTTAGATGGTCCCCTGCCTGAAAACATGTTGATTGGTCCGCGTGTAGGCATCAACTATGCTTTGCCTGAGCACGTCAATGCGTTGTGGAGATTTGCAATTGCAGGTACCCCTTGGATAAGTTCTCCGAAGAACACTCTCAGGCCACCGTCATCTTAA

Protein Analysis

263

Amino Acids

28.47

Weight (kDa)

9.4

Isoelectric Point (pI)

49.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pur_DNA_glyco PF02245 70 - 247 6e-52 Methylpurine-DNA glycosylase (MPG)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 725
Acc65I GGTACC 1 cut(s) 737
AccB1I GGYRCC 1 cut(s) 737
AccB7I CCANNNNNTGG 1 cut(s) 488
AccII CGCG 4 cut(s) 16, 48, 241, 673
AciI CCGC 7 cut(s) 14, 98, 131, 239, 321, 455, 671
AcuI CTGAAG 1 cut(s) 266
AcyI GRCGYC 1 cut(s) 222
AfaI GTAC 1 cut(s) 739
AfiI CCNNNNNNNGG 4 cut(s) 70, 368, 488, 677
AflIII ACRYGT 1 cut(s) 657
AgsI TTSAA 4 cut(s) 22, 299, 417, 629
AhlI ACTAGT 1 cut(s) 527
AjiI CACGTC 1 cut(s) 706
AjnI CCWGG 4 cut(s) 373, 553, 573, 616
AluBI AGCT 2 cut(s) 11, 302
AluI AGCT 2 cut(s) 11, 302
Alw21I GWGCWC 2 cut(s) 13, 705
Alw26I GTCTC 2 cut(s) 261, 491
AlwNI CAGNNNCTG 1 cut(s) 362
AoxI GGCC 1 cut(s) 773
ApeKI GCWGC 1 cut(s) 128
Asp718I GGTACC 1 cut(s) 737
AspLEI GCGC 3 cut(s) 48, 50, 479
AspS9I GGNCC 4 cut(s) 371, 551, 641, 668
AvaII GGWCC 4 cut(s) 371, 551, 641, 668
AxyI CCTNAGG 1 cut(s) 260
BanI GGYRCC 1 cut(s) 737
BanII GRGCYC 1 cut(s) 13
BbsI GAAGAC 2 cut(s) 401, 530
Bbv12I GWGCWC 2 cut(s) 13, 705
BbvI GCAGC 1 cut(s) 140
BccI CCATC 2 cut(s) 200, 632
BceAI ACGGC 1 cut(s) 97
BciT130I CCWGG 4 cut(s) 375, 555, 575, 618
BcoDI GTCTC 2 cut(s) 261, 491
BcuI ACTAGT 1 cut(s) 527
BfaI CTAG 2 cut(s) 227, 528
BfmI CTRYAG 1 cut(s) 585
BfuAI ACCTGC 1 cut(s) 725
BisI GCNGC 2 cut(s) 129, 456
BlsI GCNGC 2 cut(s) 130, 457
Bme1390I CCNGG 4 cut(s) 375, 555, 575, 618
Bme18I GGWCC 4 cut(s) 371, 551, 641, 668
BmgBI CACGTC 1 cut(s) 706
BmgT120I GGNCC 4 cut(s) 371, 551, 641, 668
BmiI GGNNCC 2 cut(s) 643, 739
BmrFI CCNGG 4 cut(s) 375, 555, 575, 618
BmrI ACTGGG 1 cut(s) 109
BmsI GCATC 1 cut(s) 690
BmuI ACTGGG 1 cut(s) 109
BpiI GAAGAC 2 cut(s) 401, 530
Bpu10I CCTNAGC 1 cut(s) 699
BsaAI YACGTR 1 cut(s) 470
BsaHI GRCGYC 1 cut(s) 222
BsaI GGTCTC 1 cut(s) 491
BsaJI CCNNGG 4 cut(s) 291, 573, 574, 743
Bsc4I CCNNNNNNNGG 4 cut(s) 70, 368, 488, 677
Bse1I ACTGG 1 cut(s) 115
Bse21I CCTNAGG 1 cut(s) 260
BseBI CCWGG 4 cut(s) 375, 555, 575, 618
BseDI CCNNGG 4 cut(s) 291, 573, 574, 743
BseGI GGATG 1 cut(s) 79
BseLI CCNNNNNNNGG 4 cut(s) 70, 368, 488, 677
BseMII CTCAG 3 cut(s) 82, 690, 784
BseNI ACTGG 1 cut(s) 115
BsePI GCGCGC 1 cut(s) 46
BseXI GCAGC 1 cut(s) 140
Bsh1236I CGCG 4 cut(s) 16, 48, 241, 673
BshFI GGCC 1 cut(s) 775
BshNI GGYRCC 1 cut(s) 737
BsiHKAI GWGCWC 2 cut(s) 13, 705
BslFI GGGAC 2 cut(s) 591, 627
BslI CCNNNNNNNGG 4 cut(s) 70, 368, 488, 677
BsmAI GTCTC 2 cut(s) 261, 491
BsmBI CGTCTC 1 cut(s) 261
BsmFI GGGAC 2 cut(s) 591, 627
BsnI GGCC 1 cut(s) 775
Bso31I GGTCTC 1 cut(s) 491
Bsp1286I GDGCHC 2 cut(s) 13, 705
Bsp143I GATC 1 cut(s) 561
BspACI CCGC 7 cut(s) 14, 98, 131, 239, 321, 455, 671
BspANI GGCC 1 cut(s) 775
BspCNI CTCAG 3 cut(s) 81, 691, 783
BspFNI CGCG 4 cut(s) 16, 48, 241, 673
BspLI GGNNCC 2 cut(s) 643, 739
BspMI ACCTGC 1 cut(s) 725
BspT107I GGYRCC 1 cut(s) 737
BspTNI GGTCTC 1 cut(s) 491
BsrI ACTGG 1 cut(s) 115
BssECI CCNNGG 4 cut(s) 291, 573, 574, 743
BssHII GCGCGC 1 cut(s) 46
BssMI GATC 1 cut(s) 561
BssNI GRCGYC 1 cut(s) 222
BssT1I CCWWGG 1 cut(s) 743
Bst2UI CCWGG 4 cut(s) 375, 555, 575, 618
Bst4CI ACNGT 1 cut(s) 780
BstACI GRCGYC 1 cut(s) 222
BstBAI YACGTR 1 cut(s) 470
BstC8I GCNNGC 3 cut(s) 48, 384, 475
BstDEI CTNAG 6 cut(s) 68, 260, 545, 634, 699, 770
BstF5I GGATG 1 cut(s) 79
BstFNI CGCG 4 cut(s) 16, 48, 241, 673
BstHHI GCGC 3 cut(s) 48, 50, 479
BstKTI GATC 1 cut(s) 564
BstMAI GTCTC 2 cut(s) 261, 491
BstMBI GATC 1 cut(s) 561
BstNI CCWGG 4 cut(s) 375, 555, 575, 618
BstNSI RCATGY 1 cut(s) 661
BstSCI CCNGG 4 cut(s) 373, 553, 573, 616
BstSFI CTRYAG 1 cut(s) 585
BstUI CGCG 4 cut(s) 16, 48, 241, 673
BstV1I GCAGC 1 cut(s) 140
BstV2I GAAGAC 2 cut(s) 401, 530
Bsu36I CCTNAGG 1 cut(s) 260
BsuRI GGCC 1 cut(s) 775
BtrI CACGTC 1 cut(s) 706
BtsCI GGATG 1 cut(s) 79
BveI ACCTGC 1 cut(s) 725
Cac8I GCNNGC 3 cut(s) 48, 384, 475
CaiI CAGNNNCTG 1 cut(s) 362
CfoI GCGC 3 cut(s) 48, 50, 479
Cfr13I GGNCC 4 cut(s) 371, 551, 641, 668
CseI GACGC 1 cut(s) 230
Csp6I GTAC 1 cut(s) 738
CviAII CATG 2 cut(s) 329, 658
CviJI RGCY 7 cut(s) 11, 302, 359, 458, 535, 572, 775
CviKI_1 RGCY 7 cut(s) 11, 302, 359, 458, 535, 572, 775
CviQI GTAC 1 cut(s) 738
DdeI CTNAG 6 cut(s) 68, 260, 545, 634, 699, 770
DpnI GATC 1 cut(s) 563
DpnII GATC 1 cut(s) 561
EciI GGCGGA 1 cut(s) 87
Ecl136II GAGCTC 1 cut(s) 11
Eco130I CCWWGG 1 cut(s) 743
Eco24I GRGCYC 1 cut(s) 13
Eco31I GGTCTC 1 cut(s) 491
Eco47I GGWCC 4 cut(s) 371, 551, 641, 668
Eco53kI GAGCTC 1 cut(s) 11
Eco57I CTGAAG 1 cut(s) 266
Eco81I CCTNAGG 1 cut(s) 260
EcoICRI GAGCTC 1 cut(s) 11
EcoRII CCWGG 4 cut(s) 373, 553, 573, 616
EcoT14I CCWWGG 1 cut(s) 743
EcoT38I GRGCYC 1 cut(s) 13
ErhI CCWWGG 1 cut(s) 743
Esp3I CGTCTC 1 cut(s) 261
FaeI CATG 2 cut(s) 332, 661
FaiI YATR 7 cut(s) 330, 344, 390, 405, 503, 659, 690
FaqI GGGAC 2 cut(s) 591, 627
FatI CATG 2 cut(s) 328, 657
FauI CCCGC 1 cut(s) 246
Fnu4HI GCNGC 2 cut(s) 129, 456
FokI GGATG 1 cut(s) 86
FriOI GRGCYC 1 cut(s) 13
Fsp4HI GCNGC 2 cut(s) 129, 456
FspBI CTAG 2 cut(s) 227, 528
GlaI GCGC 3 cut(s) 47, 49, 478
GluI GCNGC 2 cut(s) 129, 456
HaeIII GGCC 1 cut(s) 775
HgaI GACGC 1 cut(s) 230
HhaI GCGC 3 cut(s) 48, 50, 479
Hin1I GRCGYC 1 cut(s) 222
Hin1II CATG 2 cut(s) 332, 661
Hin6I GCGC 3 cut(s) 46, 48, 477
HinP1I GCGC 3 cut(s) 46, 48, 477
HincII GTYRAC 2 cut(s) 107, 123
HindII GTYRAC 2 cut(s) 107, 123
HindIII AAGCTT 1 cut(s) 300
HinfI GANTC 7 cut(s) 18, 27, 103, 152, 172, 317, 447
Hpy166II GTNNAC 3 cut(s) 107, 123, 551
Hpy188I TCNGA 3 cut(s) 285, 566, 759
Hpy188III TCNNGA 3 cut(s) 179, 214, 626
Hpy8I GTNNAC 3 cut(s) 107, 123, 551
Hpy99I CGWCG 4 cut(s) 83, 89, 275, 516
HpyAV CCTTC 1 cut(s) 120
HpyCH4III ACNGT 1 cut(s) 780
HpyCH4IV ACGT 4 cut(s) 273, 469, 511, 705
HpyCH4V TGCA 2 cut(s) 728, 734
HpyF3I CTNAG 6 cut(s) 68, 260, 545, 634, 699, 770
HpySE526I ACGT 4 cut(s) 273, 469, 511, 705
Hsp92I GRCGYC 1 cut(s) 222
Hsp92II CATG 2 cut(s) 332, 661
HspAI GCGC 3 cut(s) 46, 48, 477
KpnI GGTACC 1 cut(s) 741
Kzo9I GATC 1 cut(s) 561
LmnI GCTCC 2 cut(s) 16, 484
Lsp1109I GCAGC 1 cut(s) 140
LweI GCATC 1 cut(s) 690
MaeI CTAG 2 cut(s) 227, 528
MaeII ACGT 4 cut(s) 273, 469, 511, 705
MalI GATC 1 cut(s) 563
MboI GATC 1 cut(s) 561
MboII GAAGA 6 cut(s) 16, 202, 272, 401, 535, 772
MfeI CAATTG 1 cut(s) 729
MhlI GDGCHC 2 cut(s) 13, 705
MluCI AATT 2 cut(s) 483, 729
MlyI GAGTC 3 cut(s) 112, 146, 456
MnlI CCTC 7 cut(s) 186, 242, 286, 371, 436, 509, 617
MseI TTAA 1 cut(s) 787
MslI CAYNNNNRTG 1 cut(s) 708
MspA1I CMGCKG 1 cut(s) 131
MspR9I CCNGG 4 cut(s) 375, 555, 575, 618
MunI CAATTG 1 cut(s) 729
MvaI CCWGG 4 cut(s) 375, 555, 575, 618
MvnI CGCG 4 cut(s) 16, 48, 241, 673
NdeII GATC 1 cut(s) 561
NlaIII CATG 2 cut(s) 332, 661
NlaIV GGNNCC 2 cut(s) 643, 739
NmeAIII GCCGAG 2 cut(s) 126, 228
NspI RCATGY 1 cut(s) 661
PasI CCCWGGG 1 cut(s) 574
PauI GCGCGC 1 cut(s) 46
PciI ACATGT 1 cut(s) 657
PcsI WCGNNNNNNNCGW 1 cut(s) 84
PfeI GAWTC 4 cut(s) 18, 27, 172, 317
PflMI CCANNNNNTGG 1 cut(s) 488
PkrI GCNGC 2 cut(s) 130, 457
PleI GAGTC 3 cut(s) 111, 146, 455
PpsI GAGTC 3 cut(s) 111, 146, 455
Ppu21I YACGTR 1 cut(s) 470
PscI ACATGT 1 cut(s) 657
Psp124BI GAGCTC 1 cut(s) 13
Psp6I CCWGG 4 cut(s) 373, 553, 573, 616
PspGI CCWGG 4 cut(s) 373, 553, 573, 616
PspN4I GGNNCC 2 cut(s) 643, 739
PspPI GGNCC 4 cut(s) 371, 551, 641, 668
PstNI CAGNNNCTG 1 cut(s) 362
PteI GCGCGC 1 cut(s) 46
RsaI GTAC 1 cut(s) 739
RsaNI GTAC 1 cut(s) 738
RseI CAYNNNNRTG 1 cut(s) 708
SacI GAGCTC 1 cut(s) 13
SaqAI TTAA 1 cut(s) 787
SatI GCNGC 2 cut(s) 129, 456
Sau3AI GATC 1 cut(s) 561
Sau96I GGNCC 4 cut(s) 371, 551, 641, 668
SchI GAGTC 3 cut(s) 112, 146, 456
ScrFI CCNGG 4 cut(s) 375, 555, 575, 618
SduI GDGCHC 2 cut(s) 13, 705
SfaNI GCATC 1 cut(s) 690
SfcI CTRYAG 1 cut(s) 585
SinI GGWCC 4 cut(s) 371, 551, 641, 668
SmiMI CAYNNNNRTG 1 cut(s) 708
SpeI ACTAGT 1 cut(s) 527
Sse9I AATT 2 cut(s) 483, 729
SsiI CCGC 7 cut(s) 14, 98, 131, 239, 321, 455, 671
SspMI CTAG 2 cut(s) 227, 528
SstI GAGCTC 1 cut(s) 13
StyD4I CCNGG 4 cut(s) 373, 553, 573, 616
StyI CCWWGG 1 cut(s) 743
TaaI ACNGT 1 cut(s) 780
TaiI ACGT 4 cut(s) 276, 472, 514, 708
TaqI TCGA 2 cut(s) 334, 514
TasI AATT 2 cut(s) 483, 729
TauI GCSGC 1 cut(s) 458
TfiI GAWTC 4 cut(s) 18, 27, 172, 317
Tru1I TTAA 1 cut(s) 787
Tru9I TTAA 1 cut(s) 787
TseI GCWGC 1 cut(s) 128
TspDTI ATGAA 2 cut(s) 17, 401
Van91I CCANNNNNTGG 1 cut(s) 488
VpaK11BI GGWCC 4 cut(s) 371, 551, 641, 668
XceI RCATGY 1 cut(s) 661
XspI CTAG 2 cut(s) 227, 528
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.