Rroxscaffold_6G00402440

DNA-3-methyladenine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0008717
Physical Location & Seq
Reverse (-)
24914288 .. 24917375
3088 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00402440.1

Sequence Viewer

Length: 753 bp
ATGAAGAGAGCTCCGCGATTCAAACGAGTCGCCGCTAAAACCCCCAAGCGGCCAACACTCTCAAACCGACCCAGCGATGACGACGACCAAACTCCGCCGAGTCAACCCCAACCCGACTCAACTCCAATCCCAGAGATTCCTGCGGATAAAATGACCATCTTGCCCTACACATTCTTCCAGATTGACGCCCTAGACCTCGCCCCGCGTTTGCTCGGCAAGTTCCTTAGGAGAGACGACGTTGTTCTTCAGATTACCGAGGTAGAAGCTTATAGACCAAATGATTCGGCTTGTCACGGTCGATTTGGCATAACAACAAGAACGGCCCCTGTTTTTGGACCAGGAGGGCATGCATATGTTTATCTTTGCTATGGTCTTCATTCGATGCTGAATGTTGTTGCTGACAAGGAGGGAGTCGGGGCGGCTGTCCTAATACGCGCTTGCGCTCCAATTAGTGGATTGGAGACCATACAGCAACGTCGAGGGCAGAAGACTAGTAAGCCTGTTCTCCTTAGTGGACCAGGAAAGATCGGGCAAGCCCTGGGACTTTCTACAGAATGGTCTAACCACCCCCTTTACACTCCTGGTGGTCTGGAAATCTTAGATGGTCCCCAGCCTGAAAACATGTTGATTGGTCCGCGTGTAGGCATCAACTATGCTTTGCCTGAACACGTCAATGCGTTGTGGAGATTTGCAATTGCAGGTACCCCTTGGATAAGTGCTCCGAAGAACACTCTCAGGCCACCGTCATCTTAA

Protein Analysis

250

Amino Acids

27.23

Weight (kDa)

9.3

Isoelectric Point (pI)

48.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pur_DNA_glyco PF02245 58 - 235 9.8e-53 Methylpurine-DNA glycosylase (MPG)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 689
Acc65I GGTACC 1 cut(s) 701
AccB1I GGYRCC 1 cut(s) 701
AccB7I CCANNNNNTGG 1 cut(s) 452
AccII CGCG 4 cut(s) 16, 205, 435, 637
AciI CCGC 8 cut(s) 14, 33, 49, 95, 143, 203, 419, 635
AcoI YGGCCR 1 cut(s) 50
AcuI CTGAAG 1 cut(s) 230
AcyI GRCGYC 1 cut(s) 186
AfaI GTAC 1 cut(s) 703
AfiI CCNNNNNNNGG 4 cut(s) 48, 332, 452, 641
AflIII ACRYGT 2 cut(s) 621, 667
AgsI TTSAA 1 cut(s) 22
AhlI ACTAGT 1 cut(s) 491
AjiI CACGTC 1 cut(s) 670
AjnI CCWGG 4 cut(s) 337, 517, 537, 580
AluBI AGCT 2 cut(s) 11, 266
AluI AGCT 2 cut(s) 11, 266
Alw21I GWGCWC 2 cut(s) 13, 721
Alw26I GTCTC 2 cut(s) 225, 455
AoxI GGCC 3 cut(s) 50, 321, 737
Asp718I GGTACC 1 cut(s) 701
AspLEI GCGC 2 cut(s) 437, 443
AspS9I GGNCC 5 cut(s) 322, 335, 515, 605, 632
AvaII GGWCC 4 cut(s) 335, 515, 605, 632
AxyI CCTNAGG 1 cut(s) 224
BanI GGYRCC 1 cut(s) 701
BanII GRGCYC 1 cut(s) 13
BbsI GAAGAC 2 cut(s) 365, 494
Bbv12I GWGCWC 2 cut(s) 13, 721
BccI CCATC 2 cut(s) 164, 596
BceAI ACGGC 1 cut(s) 336
BciT130I CCWGG 4 cut(s) 339, 519, 539, 582
BcoDI GTCTC 2 cut(s) 225, 455
BcuI ACTAGT 1 cut(s) 491
BfaI CTAG 2 cut(s) 191, 492
BfmI CTRYAG 1 cut(s) 549
BfuAI ACCTGC 1 cut(s) 689
BisI GCNGC 3 cut(s) 33, 50, 420
BlsI GCNGC 3 cut(s) 34, 51, 421
Bme1390I CCNGG 4 cut(s) 339, 519, 539, 582
Bme18I GGWCC 4 cut(s) 335, 515, 605, 632
BmgBI CACGTC 1 cut(s) 670
BmgT120I GGNCC 5 cut(s) 322, 335, 515, 605, 632
BmiI GGNNCC 3 cut(s) 324, 607, 703
BmrFI CCNGG 4 cut(s) 339, 519, 539, 582
BmsI GCATC 2 cut(s) 372, 654
BpiI GAAGAC 2 cut(s) 365, 494
BsaHI GRCGYC 1 cut(s) 186
BsaI GGTCTC 1 cut(s) 455
BsaJI CCNNGG 4 cut(s) 255, 537, 538, 707
Bsc4I CCNNNNNNNGG 4 cut(s) 48, 332, 452, 641
Bse21I CCTNAGG 1 cut(s) 224
BseBI CCWGG 4 cut(s) 339, 519, 539, 582
BseDI CCNNGG 4 cut(s) 255, 537, 538, 707
BseLI CCNNNNNNNGG 4 cut(s) 48, 332, 452, 641
BseMII CTCAG 1 cut(s) 748
BseYI CCCAGC 2 cut(s) 71, 609
Bsh1236I CGCG 4 cut(s) 16, 205, 435, 637
Bsh1285I CGRYCG 1 cut(s) 298
BshFI GGCC 3 cut(s) 52, 323, 739
BshNI GGYRCC 1 cut(s) 701
BsiEI CGRYCG 1 cut(s) 298
BsiHKAI GWGCWC 2 cut(s) 13, 721
BslFI GGGAC 2 cut(s) 555, 591
BslI CCNNNNNNNGG 4 cut(s) 48, 332, 452, 641
BsmAI GTCTC 2 cut(s) 225, 455
BsmBI CGTCTC 1 cut(s) 225
BsmFI GGGAC 2 cut(s) 555, 591
BsnI GGCC 3 cut(s) 52, 323, 739
Bso31I GGTCTC 1 cut(s) 455
Bsp1286I GDGCHC 2 cut(s) 13, 721
Bsp143I GATC 1 cut(s) 525
BspACI CCGC 8 cut(s) 14, 33, 49, 95, 143, 203, 419, 635
BspANI GGCC 3 cut(s) 52, 323, 739
BspCNI CTCAG 1 cut(s) 747
BspFNI CGCG 4 cut(s) 16, 205, 435, 637
BspLI GGNNCC 3 cut(s) 324, 607, 703
BspMI ACCTGC 1 cut(s) 689
BspT107I GGYRCC 1 cut(s) 701
BspTNI GGTCTC 1 cut(s) 455
BssECI CCNNGG 4 cut(s) 255, 537, 538, 707
BssMI GATC 1 cut(s) 525
BssNI GRCGYC 1 cut(s) 186
BssT1I CCWWGG 1 cut(s) 707
Bst2UI CCWGG 4 cut(s) 339, 519, 539, 582
Bst4CI ACNGT 2 cut(s) 296, 744
BstACI GRCGYC 1 cut(s) 186
BstC8I GCNNGC 3 cut(s) 348, 439, 534
BstDEI CTNAG 4 cut(s) 224, 509, 598, 734
BstFNI CGCG 4 cut(s) 16, 205, 435, 637
BstHHI GCGC 2 cut(s) 437, 443
BstKTI GATC 1 cut(s) 528
BstMAI GTCTC 2 cut(s) 225, 455
BstMBI GATC 1 cut(s) 525
BstMCI CGRYCG 1 cut(s) 298
BstNI CCWGG 4 cut(s) 339, 519, 539, 582
BstNSI RCATGY 2 cut(s) 350, 625
BstSCI CCNGG 4 cut(s) 337, 517, 537, 580
BstSFI CTRYAG 1 cut(s) 549
BstUI CGCG 4 cut(s) 16, 205, 435, 637
BstV2I GAAGAC 2 cut(s) 365, 494
Bsu36I CCTNAGG 1 cut(s) 224
BsuRI GGCC 3 cut(s) 52, 323, 739
BtgZI GCGATG 1 cut(s) 90
BtrI CACGTC 1 cut(s) 670
BveI ACCTGC 1 cut(s) 689
Cac8I GCNNGC 3 cut(s) 348, 439, 534
CfoI GCGC 2 cut(s) 437, 443
Cfr13I GGNCC 5 cut(s) 322, 335, 515, 605, 632
CseI GACGC 1 cut(s) 194
Csp6I GTAC 1 cut(s) 702
CviAII CATG 2 cut(s) 347, 622
CviQI GTAC 1 cut(s) 702
DdeI CTNAG 4 cut(s) 224, 509, 598, 734
DpnI GATC 1 cut(s) 527
DpnII GATC 1 cut(s) 525
EaeI YGGCCR 1 cut(s) 50
EciI GGCGGA 1 cut(s) 84
Ecl136II GAGCTC 1 cut(s) 11
Eco130I CCWWGG 1 cut(s) 707
Eco24I GRGCYC 1 cut(s) 13
Eco31I GGTCTC 1 cut(s) 455
Eco47I GGWCC 4 cut(s) 335, 515, 605, 632
Eco53kI GAGCTC 1 cut(s) 11
Eco57I CTGAAG 1 cut(s) 230
Eco81I CCTNAGG 1 cut(s) 224
EcoICRI GAGCTC 1 cut(s) 11
EcoRII CCWGG 4 cut(s) 337, 517, 537, 580
EcoT14I CCWWGG 1 cut(s) 707
EcoT22I ATGCAT 1 cut(s) 352
EcoT38I GRGCYC 1 cut(s) 13
ErhI CCWWGG 1 cut(s) 707
Esp3I CGTCTC 1 cut(s) 225
FaeI CATG 2 cut(s) 350, 625
FaiI YATR 9 cut(s) 270, 308, 348, 352, 354, 369, 467, 623, 654
FaqI GGGAC 2 cut(s) 555, 591
FatI CATG 2 cut(s) 346, 621
FauI CCCGC 1 cut(s) 210
FauNDI CATATG 1 cut(s) 352
Fnu4HI GCNGC 3 cut(s) 33, 50, 420
FriOI GRGCYC 1 cut(s) 13
Fsp4HI GCNGC 3 cut(s) 33, 50, 420
FspBI CTAG 2 cut(s) 191, 492
GlaI GCGC 2 cut(s) 436, 442
GluI GCNGC 3 cut(s) 33, 50, 420
GsaI CCCAGC 2 cut(s) 75, 613
HaeIII GGCC 3 cut(s) 52, 323, 739
HgaI GACGC 1 cut(s) 194
HhaI GCGC 2 cut(s) 437, 443
Hin1I GRCGYC 1 cut(s) 186
Hin1II CATG 2 cut(s) 350, 625
Hin6I GCGC 2 cut(s) 435, 441
HinP1I GCGC 2 cut(s) 435, 441
HincII GTYRAC 1 cut(s) 104
HindII GTYRAC 1 cut(s) 104
HindIII AAGCTT 1 cut(s) 264
HinfI GANTC 7 cut(s) 18, 27, 100, 116, 136, 281, 411
Hpy166II GTNNAC 2 cut(s) 104, 515
Hpy188I TCNGA 2 cut(s) 249, 723
Hpy188III TCNNGA 2 cut(s) 178, 590
Hpy8I GTNNAC 2 cut(s) 104, 515
Hpy99I CGWCG 3 cut(s) 86, 239, 480
HpyCH4III ACNGT 2 cut(s) 296, 744
HpyCH4IV ACGT 3 cut(s) 237, 475, 669
HpyCH4V TGCA 3 cut(s) 350, 692, 698
HpyF3I CTNAG 4 cut(s) 224, 509, 598, 734
HpySE526I ACGT 3 cut(s) 237, 475, 669
Hsp92I GRCGYC 1 cut(s) 186
Hsp92II CATG 2 cut(s) 350, 625
HspAI GCGC 2 cut(s) 435, 441
KpnI GGTACC 1 cut(s) 705
Kzo9I GATC 1 cut(s) 525
LmnI GCTCC 3 cut(s) 16, 448, 724
LweI GCATC 2 cut(s) 372, 654
MaeI CTAG 2 cut(s) 191, 492
MaeII ACGT 3 cut(s) 237, 475, 669
MaeIII GTNAC 1 cut(s) 290
MalI GATC 1 cut(s) 527
MboI GATC 1 cut(s) 525
MboII GAAGA 6 cut(s) 16, 166, 236, 365, 499, 736
MfeI CAATTG 1 cut(s) 693
MhlI GDGCHC 2 cut(s) 13, 721
MluCI AATT 2 cut(s) 447, 693
MlyI GAGTC 4 cut(s) 36, 109, 110, 420
MnlI CCTC 5 cut(s) 206, 250, 335, 400, 473
Mph1103I ATGCAT 1 cut(s) 352
MseI TTAA 1 cut(s) 751
MslI CAYNNNNRTG 2 cut(s) 351, 672
MspR9I CCNGG 4 cut(s) 339, 519, 539, 582
MunI CAATTG 1 cut(s) 693
MvaI CCWGG 4 cut(s) 339, 519, 539, 582
MvnI CGCG 4 cut(s) 16, 205, 435, 637
NdeI CATATG 1 cut(s) 352
NdeII GATC 1 cut(s) 525
NlaIII CATG 2 cut(s) 350, 625
NlaIV GGNNCC 3 cut(s) 324, 607, 703
NmeAIII GCCGAG 2 cut(s) 123, 192
NmuCI GTSAC 1 cut(s) 290
NsiI ATGCAT 1 cut(s) 352
NspI RCATGY 2 cut(s) 350, 625
PaeI GCATGC 1 cut(s) 350
PasI CCCWGGG 1 cut(s) 538
PciI ACATGT 1 cut(s) 621
PfeI GAWTC 3 cut(s) 18, 136, 281
PflMI CCANNNNNTGG 1 cut(s) 452
PkrI GCNGC 3 cut(s) 34, 51, 421
PleI GAGTC 4 cut(s) 35, 108, 110, 419
PpsI GAGTC 4 cut(s) 35, 108, 110, 419
PscI ACATGT 1 cut(s) 621
Psp124BI GAGCTC 1 cut(s) 13
Psp6I CCWGG 4 cut(s) 337, 517, 537, 580
PspFI CCCAGC 2 cut(s) 71, 609
PspGI CCWGG 4 cut(s) 337, 517, 537, 580
PspN4I GGNNCC 3 cut(s) 324, 607, 703
PspPI GGNCC 5 cut(s) 322, 335, 515, 605, 632
RsaI GTAC 1 cut(s) 703
RsaNI GTAC 1 cut(s) 702
RseI CAYNNNNRTG 2 cut(s) 351, 672
SacI GAGCTC 1 cut(s) 13
SaqAI TTAA 1 cut(s) 751
SatI GCNGC 3 cut(s) 33, 50, 420
Sau3AI GATC 1 cut(s) 525
Sau96I GGNCC 5 cut(s) 322, 335, 515, 605, 632
SchI GAGTC 4 cut(s) 36, 109, 110, 420
ScrFI CCNGG 4 cut(s) 339, 519, 539, 582
SduI GDGCHC 2 cut(s) 13, 721
SetI ASST 8 cut(s) 13, 198, 240, 261, 268, 478, 672, 703
SfaNI GCATC 2 cut(s) 372, 654
SfcI CTRYAG 1 cut(s) 549
SinI GGWCC 4 cut(s) 335, 515, 605, 632
SmiMI CAYNNNNRTG 2 cut(s) 351, 672
SpeI ACTAGT 1 cut(s) 491
SphI GCATGC 1 cut(s) 350
Sse9I AATT 2 cut(s) 447, 693
SsiI CCGC 8 cut(s) 14, 33, 49, 95, 143, 203, 419, 635
SspMI CTAG 2 cut(s) 191, 492
SstI GAGCTC 1 cut(s) 13
StyD4I CCNGG 4 cut(s) 337, 517, 537, 580
StyI CCWWGG 1 cut(s) 707
TaaI ACNGT 2 cut(s) 296, 744
TaiI ACGT 3 cut(s) 240, 478, 672
TaqI TCGA 3 cut(s) 298, 380, 478
TasI AATT 2 cut(s) 447, 693
TauI GCSGC 3 cut(s) 35, 52, 422
TfiI GAWTC 3 cut(s) 18, 136, 281
Tru1I TTAA 1 cut(s) 751
Tru9I TTAA 1 cut(s) 751
TseFI GTSAC 1 cut(s) 290
Tsp45I GTSAC 1 cut(s) 290
TspDTI ATGAA 2 cut(s) 17, 365
Van91I CCANNNNNTGG 1 cut(s) 452
VpaK11BI GGWCC 4 cut(s) 335, 515, 605, 632
XceI RCATGY 2 cut(s) 350, 625
XspI CTAG 2 cut(s) 191, 492
Zsp2I ATGCAT 1 cut(s) 352
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.