RchiOBHm_Chr3g0479281

DNA-3-methyladenine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0008717
Physical Location & Seq
Forward (+)
25081900 .. 25084839
2940 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ44438

Sequence Viewer

Length: 762 bp
ATGAAGAGAGCTCCGCGATTCAAACGAGTCGCCGCTAAAACCCCCAAGCGGCCAACACTCTCAAACCGACCCAGCGATGACGACGACGACGACGACCAAACTCCGCCGAGTCAACCCGAACCCGACTCAACTCCAATCCCAGAGATTCCTGCGGATAAAATGACCATCTTGCCCTACACATTCTTCCAGATTGACGCCCTAGACCTCGCCCCGCGTTTGCTCGGCAAGTTCCTTAGGAGAGACGACGTTGTTCTTCAGATTACCGAGGTTGAAGCTTACAGACCAAATGATTCGGCTTGTCACGGTCGATTTGGCATAACACCAAGAACAGCCCCTGTTTTTGGACCAGGAGGGCGTGCTTATGTTTATCTTTGCTATGGTCTTCATTCGATGCTGAATGTTGTTGCTGACAAGGAGGGAGTCGGGGCGGCTGTTCTAATACGTGCTTGCGCTCCAATTAGTGGATTGGAGACCATACAGCAACGTCGAGGGCAGAAGACTAGTAAGCCTGTTCTCCTTAGTGGACCAGGAAAGATCGGTCAAGCCCTGGGACTTTCTACAGAATGGTCTAACCACCCCCTCTACACTCCTGGTGGTCTGGAAATCTTAGATGGTCCCCCACCTGAAAACATGTTGATTGGTCCGCGTGTAGGCATCAACTATGCTTTGCCTGAGCACGTCAATGCGTTGTGGAGATTTGCAATTGCAGGTACCCCTTGGATAAGTGCTCCGAAGAACACTCTCCGGCCACCGTCATCTTAA

Protein Analysis

253

Amino Acids

27.56

Weight (kDa)

8.74

Isoelectric Point (pI)

47.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pur_DNA_glyco PF02245 61 - 238 1.4e-52 Methylpurine-DNA glycosylase (MPG)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 698
Acc65I GGTACC 1 cut(s) 710
AccB1I GGYRCC 1 cut(s) 710
AccB7I CCANNNNNTGG 1 cut(s) 461
AccII CGCG 3 cut(s) 16, 214, 646
AciI CCGC 8 cut(s) 14, 33, 49, 104, 152, 212, 428, 644
AcoI YGGCCR 2 cut(s) 50, 746
AcuI CTGAAG 1 cut(s) 239
AcyI GRCGYC 1 cut(s) 195
AfaI GTAC 1 cut(s) 712
AfiI CCNNNNNNNGG 4 cut(s) 48, 341, 461, 650
AflIII ACRYGT 1 cut(s) 630
AgsI TTSAA 2 cut(s) 22, 272
AhlI ACTAGT 1 cut(s) 500
AjiI CACGTC 1 cut(s) 679
AjnI CCWGG 4 cut(s) 346, 526, 546, 589
AluBI AGCT 2 cut(s) 11, 275
AluI AGCT 2 cut(s) 11, 275
Alw21I GWGCWC 3 cut(s) 13, 678, 730
Alw26I GTCTC 2 cut(s) 234, 464
AlwNI CAGNNNCTG 1 cut(s) 335
AoxI GGCC 2 cut(s) 50, 746
Asp718I GGTACC 1 cut(s) 710
AspLEI GCGC 1 cut(s) 452
AspS9I GGNCC 4 cut(s) 344, 524, 614, 641
AvaII GGWCC 4 cut(s) 344, 524, 614, 641
AxyI CCTNAGG 1 cut(s) 233
BanI GGYRCC 1 cut(s) 710
BanII GRGCYC 1 cut(s) 13
BbsI GAAGAC 2 cut(s) 374, 503
Bbv12I GWGCWC 3 cut(s) 13, 678, 730
BccI CCATC 2 cut(s) 173, 605
BciT130I CCWGG 4 cut(s) 348, 528, 548, 591
BcoDI GTCTC 2 cut(s) 234, 464
BcuI ACTAGT 1 cut(s) 500
BfaI CTAG 2 cut(s) 200, 501
BfmI CTRYAG 1 cut(s) 558
BfuAI ACCTGC 1 cut(s) 698
BisI GCNGC 3 cut(s) 33, 50, 429
BlsI GCNGC 3 cut(s) 34, 51, 430
Bme1390I CCNGG 4 cut(s) 348, 528, 548, 591
Bme18I GGWCC 4 cut(s) 344, 524, 614, 641
BmgBI CACGTC 1 cut(s) 679
BmgT120I GGNCC 4 cut(s) 344, 524, 614, 641
BmiI GGNNCC 2 cut(s) 616, 712
BmrFI CCNGG 4 cut(s) 348, 528, 548, 591
BmsI GCATC 2 cut(s) 381, 663
BpiI GAAGAC 2 cut(s) 374, 503
Bpu10I CCTNAGC 1 cut(s) 672
BsaAI YACGTR 1 cut(s) 443
BsaHI GRCGYC 1 cut(s) 195
BsaI GGTCTC 1 cut(s) 464
BsaJI CCNNGG 4 cut(s) 264, 546, 547, 716
Bsc4I CCNNNNNNNGG 4 cut(s) 48, 341, 461, 650
Bse21I CCTNAGG 1 cut(s) 233
BseBI CCWGG 4 cut(s) 348, 528, 548, 591
BseDI CCNNGG 4 cut(s) 264, 546, 547, 716
BseLI CCNNNNNNNGG 4 cut(s) 48, 341, 461, 650
BseMII CTCAG 1 cut(s) 663
BseYI CCCAGC 1 cut(s) 71
Bsh1236I CGCG 3 cut(s) 16, 214, 646
Bsh1285I CGRYCG 1 cut(s) 307
BshFI GGCC 2 cut(s) 52, 748
BshNI GGYRCC 1 cut(s) 710
BsiEI CGRYCG 1 cut(s) 307
BsiHKAI GWGCWC 3 cut(s) 13, 678, 730
BsiSI CCGG 1 cut(s) 745
BslFI GGGAC 2 cut(s) 564, 600
BslI CCNNNNNNNGG 4 cut(s) 48, 341, 461, 650
BsmAI GTCTC 2 cut(s) 234, 464
BsmBI CGTCTC 1 cut(s) 234
BsmFI GGGAC 2 cut(s) 564, 600
BsnI GGCC 2 cut(s) 52, 748
Bso31I GGTCTC 1 cut(s) 464
Bsp1286I GDGCHC 3 cut(s) 13, 678, 730
Bsp143I GATC 1 cut(s) 534
BspACI CCGC 8 cut(s) 14, 33, 49, 104, 152, 212, 428, 644
BspANI GGCC 2 cut(s) 52, 748
BspCNI CTCAG 1 cut(s) 664
BspFNI CGCG 3 cut(s) 16, 214, 646
BspLI GGNNCC 2 cut(s) 616, 712
BspMI ACCTGC 1 cut(s) 698
BspT107I GGYRCC 1 cut(s) 710
BspTNI GGTCTC 1 cut(s) 464
BssECI CCNNGG 4 cut(s) 264, 546, 547, 716
BssMI GATC 1 cut(s) 534
BssNI GRCGYC 1 cut(s) 195
BssT1I CCWWGG 1 cut(s) 716
Bst2UI CCWGG 4 cut(s) 348, 528, 548, 591
Bst4CI ACNGT 2 cut(s) 305, 753
BstACI GRCGYC 1 cut(s) 195
BstBAI YACGTR 1 cut(s) 443
BstC8I GCNNGC 2 cut(s) 357, 448
BstDEI CTNAG 4 cut(s) 233, 518, 607, 672
BstFNI CGCG 3 cut(s) 16, 214, 646
BstHHI GCGC 1 cut(s) 452
BstKTI GATC 1 cut(s) 537
BstMAI GTCTC 2 cut(s) 234, 464
BstMBI GATC 1 cut(s) 534
BstMCI CGRYCG 1 cut(s) 307
BstNI CCWGG 4 cut(s) 348, 528, 548, 591
BstNSI RCATGY 1 cut(s) 634
BstSCI CCNGG 4 cut(s) 346, 526, 546, 589
BstSFI CTRYAG 1 cut(s) 558
BstUI CGCG 3 cut(s) 16, 214, 646
BstV2I GAAGAC 2 cut(s) 374, 503
Bsu36I CCTNAGG 1 cut(s) 233
BsuRI GGCC 2 cut(s) 52, 748
BtgZI GCGATG 1 cut(s) 90
BtrI CACGTC 1 cut(s) 679
BveI ACCTGC 1 cut(s) 698
Cac8I GCNNGC 2 cut(s) 357, 448
CaiI CAGNNNCTG 1 cut(s) 335
CfoI GCGC 1 cut(s) 452
Cfr13I GGNCC 4 cut(s) 344, 524, 614, 641
CseI GACGC 1 cut(s) 203
Csp6I GTAC 1 cut(s) 711
CviAII CATG 1 cut(s) 631
CviJI RGCY 9 cut(s) 11, 52, 275, 296, 332, 431, 508, 545, 748
CviKI_1 RGCY 9 cut(s) 11, 52, 275, 296, 332, 431, 508, 545, 748
CviQI GTAC 1 cut(s) 711
DdeI CTNAG 4 cut(s) 233, 518, 607, 672
DpnI GATC 1 cut(s) 536
DpnII GATC 1 cut(s) 534
EaeI YGGCCR 2 cut(s) 50, 746
EciI GGCGGA 1 cut(s) 93
Ecl136II GAGCTC 1 cut(s) 11
Eco130I CCWWGG 1 cut(s) 716
Eco24I GRGCYC 1 cut(s) 13
Eco31I GGTCTC 1 cut(s) 464
Eco47I GGWCC 4 cut(s) 344, 524, 614, 641
Eco53kI GAGCTC 1 cut(s) 11
Eco57I CTGAAG 1 cut(s) 239
Eco81I CCTNAGG 1 cut(s) 233
EcoICRI GAGCTC 1 cut(s) 11
EcoRII CCWGG 4 cut(s) 346, 526, 546, 589
EcoT14I CCWWGG 1 cut(s) 716
EcoT38I GRGCYC 1 cut(s) 13
ErhI CCWWGG 1 cut(s) 716
Esp3I CGTCTC 1 cut(s) 234
FaeI CATG 1 cut(s) 634
FaiI YATR 6 cut(s) 317, 363, 378, 476, 632, 663
FaqI GGGAC 2 cut(s) 564, 600
FatI CATG 1 cut(s) 630
FauI CCCGC 1 cut(s) 219
Fnu4HI GCNGC 3 cut(s) 33, 50, 429
FriOI GRGCYC 1 cut(s) 13
Fsp4HI GCNGC 3 cut(s) 33, 50, 429
FspBI CTAG 2 cut(s) 200, 501
GlaI GCGC 1 cut(s) 451
GluI GCNGC 3 cut(s) 33, 50, 429
GsaI CCCAGC 1 cut(s) 75
HaeIII GGCC 2 cut(s) 52, 748
HapII CCGG 1 cut(s) 745
HgaI GACGC 1 cut(s) 203
HhaI GCGC 1 cut(s) 452
Hin1I GRCGYC 1 cut(s) 195
Hin1II CATG 1 cut(s) 634
Hin6I GCGC 1 cut(s) 450
HinP1I GCGC 1 cut(s) 450
HincII GTYRAC 1 cut(s) 113
HindII GTYRAC 1 cut(s) 113
HindIII AAGCTT 1 cut(s) 273
HinfI GANTC 7 cut(s) 18, 27, 109, 125, 145, 290, 420
HpaII CCGG 1 cut(s) 745
Hpy166II GTNNAC 2 cut(s) 113, 524
Hpy188I TCNGA 2 cut(s) 258, 732
Hpy188III TCNNGA 2 cut(s) 187, 599
Hpy8I GTNNAC 2 cut(s) 113, 524
Hpy99I CGWCG 6 cut(s) 86, 89, 92, 95, 248, 489
HpyCH4III ACNGT 2 cut(s) 305, 753
HpyCH4IV ACGT 4 cut(s) 246, 442, 484, 678
HpyCH4V TGCA 2 cut(s) 701, 707
HpyF3I CTNAG 4 cut(s) 233, 518, 607, 672
HpySE526I ACGT 4 cut(s) 246, 442, 484, 678
Hsp92I GRCGYC 1 cut(s) 195
Hsp92II CATG 1 cut(s) 634
HspAI GCGC 1 cut(s) 450
KpnI GGTACC 1 cut(s) 714
Kzo9I GATC 1 cut(s) 534
LmnI GCTCC 3 cut(s) 16, 457, 733
LweI GCATC 2 cut(s) 381, 663
MaeI CTAG 2 cut(s) 200, 501
MaeII ACGT 4 cut(s) 246, 442, 484, 678
MaeIII GTNAC 1 cut(s) 299
MalI GATC 1 cut(s) 536
MboI GATC 1 cut(s) 534
MboII GAAGA 6 cut(s) 16, 175, 245, 374, 508, 745
MfeI CAATTG 1 cut(s) 702
MhlI GDGCHC 3 cut(s) 13, 678, 730
MluCI AATT 2 cut(s) 456, 702
MlyI GAGTC 4 cut(s) 36, 118, 119, 429
MnlI CCTC 6 cut(s) 215, 259, 344, 409, 482, 590
MseI TTAA 1 cut(s) 760
MslI CAYNNNNRTG 1 cut(s) 681
MspI CCGG 1 cut(s) 745
MspR9I CCNGG 4 cut(s) 348, 528, 548, 591
MunI CAATTG 1 cut(s) 702
MvaI CCWGG 4 cut(s) 348, 528, 548, 591
MvnI CGCG 3 cut(s) 16, 214, 646
NdeII GATC 1 cut(s) 534
NlaIII CATG 1 cut(s) 634
NlaIV GGNNCC 2 cut(s) 616, 712
NmeAIII GCCGAG 2 cut(s) 132, 201
NmuCI GTSAC 1 cut(s) 299
NspI RCATGY 1 cut(s) 634
PasI CCCWGGG 1 cut(s) 547
PciI ACATGT 1 cut(s) 630
PcsI WCGNNNNNNNCGW 2 cut(s) 87, 90
PfeI GAWTC 3 cut(s) 18, 145, 290
PflMI CCANNNNNTGG 1 cut(s) 461
PkrI GCNGC 3 cut(s) 34, 51, 430
PleI GAGTC 4 cut(s) 35, 117, 119, 428
PpsI GAGTC 4 cut(s) 35, 117, 119, 428
Ppu21I YACGTR 1 cut(s) 443
PscI ACATGT 1 cut(s) 630
Psp124BI GAGCTC 1 cut(s) 13
Psp6I CCWGG 4 cut(s) 346, 526, 546, 589
PspFI CCCAGC 1 cut(s) 71
PspGI CCWGG 4 cut(s) 346, 526, 546, 589
PspN4I GGNNCC 2 cut(s) 616, 712
PspPI GGNCC 4 cut(s) 344, 524, 614, 641
PstNI CAGNNNCTG 1 cut(s) 335
RsaI GTAC 1 cut(s) 712
RsaNI GTAC 1 cut(s) 711
RseI CAYNNNNRTG 1 cut(s) 681
SacI GAGCTC 1 cut(s) 13
SaqAI TTAA 1 cut(s) 760
SatI GCNGC 3 cut(s) 33, 50, 429
Sau3AI GATC 1 cut(s) 534
Sau96I GGNCC 4 cut(s) 344, 524, 614, 641
SchI GAGTC 4 cut(s) 36, 118, 119, 429
ScrFI CCNGG 4 cut(s) 348, 528, 548, 591
SduI GDGCHC 3 cut(s) 13, 678, 730
SfaNI GCATC 2 cut(s) 381, 663
SfcI CTRYAG 1 cut(s) 558
SinI GGWCC 4 cut(s) 344, 524, 614, 641
SmiMI CAYNNNNRTG 1 cut(s) 681
SpeI ACTAGT 1 cut(s) 500
Sse9I AATT 2 cut(s) 456, 702
SsiI CCGC 8 cut(s) 14, 33, 49, 104, 152, 212, 428, 644
SspMI CTAG 2 cut(s) 200, 501
SstI GAGCTC 1 cut(s) 13
StyD4I CCNGG 4 cut(s) 346, 526, 546, 589
StyI CCWWGG 1 cut(s) 716
TaaI ACNGT 2 cut(s) 305, 753
TaiI ACGT 4 cut(s) 249, 445, 487, 681
TaqI TCGA 3 cut(s) 307, 389, 487
TaqII GACCGA 1 cut(s) 527
TasI AATT 2 cut(s) 456, 702
TauI GCSGC 3 cut(s) 35, 52, 431
TfiI GAWTC 3 cut(s) 18, 145, 290
Tru1I TTAA 1 cut(s) 760
Tru9I TTAA 1 cut(s) 760
TseFI GTSAC 1 cut(s) 299
Tsp45I GTSAC 1 cut(s) 299
TspDTI ATGAA 2 cut(s) 17, 374
Van91I CCANNNNNTGG 1 cut(s) 461
VpaK11BI GGWCC 4 cut(s) 344, 524, 614, 641
XceI RCATGY 1 cut(s) 634
XspI CTAG 2 cut(s) 200, 501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.