AT3G12080

KH-domain-like of EngA bacterial GTPase enzymes, C-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0001492
Physical Location & Seq
Forward (+)
3847743 .. 3852138
4396 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G12080.2

Sequence Viewer

Length: 1992 bp
ATGGCGAGTCTACTCGATTCGTTGACAACCAGAGGGTTCTTCACCATGCCCTCAATTTCTAGGATTTATTCTCCTCTAGCTTCTTCAACTTCATTGACTTCTTCACCATCTTCCTCTTCTTCGATTATCCCATCCTTGTCAGTTCTCTCTTACACTCACCAACACCCGCATTCCTCTCGCTTCCCTTTCCTTGTTGCAGCAACTCTGGATGGTTCCTCTGCTGAGGAAGAGCTAGATTTCGAGGAATTCGACCAATATGCTGAAGATAACTTCGCAGATGATTATTCGGATGACGAAGACGACTCCATCGATATCTCCGTTCTTGAGAAAGAAGCGAGAGATATCGTGAGAGATTATGCTACCACTCTGTCTCGCGAGCTCAAAATCGAGGATGAGACCATTGAGGGGAAGGAAACTCGTAGAAAAGGGAAAAGGCTAGCCAAAAATACACAGCAGATACCAGAGCATCTTCTCCAAAGAGTTGCTATAGTTGGAAGGCCTAATGTCGGAAAATCAGCATTGTTCAACCGCCTTGTTGGGGAGAATAGAGCTATTGTAGTGGATGAACCTGGAGTTACTAGGGATCGATTGTATGGTAGATCATATTGGGGTGATCAAGAGTTTGTGGTGGTAGACACCGGAGGAGTTATGACTGTTTCAAAGTCACCATCTGGTGTCATGGAAGAGCTGAATGTGTCAACCACCATTGGCATGGAAGGTATTCCACTAAGCTCTAGGGAAGCAGCTATTGCAAGGATGCCTTCCATGATTGAGAAACAAGCTACAGCAGCTGTCGACGAATCAGCTGTTATTATTTTCGTTGTCGATGGCCAGGCAGGGCCATCAGGTGCTGATGTGGAAATTGCAGACTGGTTGCGGAAGTACTACTCACATAAGTATATCATTCTCGCGGTGAACAAGTGTGAATCGCCACGTAAAGGACTCATGCAGGCTTCGGAATTTTGGTCTCTAGGGTTCACACCTATCCCTATCTCTGCATTGTCGGGAACTGGAACAGGAGAGCTGCTTGATCTTGTTTGTTCTGGCCTAATTAAACTCGAGATCATGGAGAACATTGAAGAGGAGGAAGAAGAAAACTACATTCCTGCCATTGCAATTATTGGTCGGCCAAATGTTGGCAAAAGTAGCATTTTAAATGCACTTGTCCGAGAGGATAGAACAATTGTTAGCCCTGTTAGTGGCACTACCCGTGATGCTATAGATGCAGAATTTACCGGACCAGATGGGGAGAAGTTTCGGCTTATAGATACAGCTGGGATCAGGAAAAAGTCATCTGTGGCTTCATCAGGCAGCACTACAGAGGCCATGTCAGTGAATCGTGCATTTCGAGCAATTCGTCGTTCTGATGTTGTTGCCCTTGTCATTGAAGCTATGGCATGCATAACAGAGCAGGATCTGAAGATTGCAGAAAGAATAGAAAGAGAAGGGAAAGGATGTCTGGTTGTTGTAAACAAATGGGATACAATACCGAACAAAAACCAAGAGACTGCAGCACATTACGAGGACGATGTTCGGGAGAAGCTCCGTTCCCTCAAATGGGCACCTATTGTTTATTCAACCGCAATAACTGGCCATAGCGTTGACAATATTGTGGTTGCTGCTGCTACGGTTCAAAAGGAGAGATCAAGAAGACTTAGTACGGCTATATTGAACCAAGTGATAAGAGAAGCAGTTGCATTTAAATCCCCTCCAAGGACCCGTGGAGGCAAGCGAGGTCGCGTTTACTATTGCACCCAGGCAGCAATAAGGCCACCGACGTTTGTTTTCTTTGTAAACGACGCAAAGCTGTTTTCGGATACGTACAGAAGGTATATGGAGAAACAGCTACGCACTGATGCAGGCTTTGCTGGGACTCCAATTCGGCTTCTTTGGCGTAGCCGTAAAAGATCTGACAAAAATGGAGGAGGTGGAGGAACAATGAGAATGGCAGGCCTTACACGTCAGAGAAATCTTGCAACCAAAAGAACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KH_dom-like MMR_HSR1

Protein Analysis

663

Amino Acids

73.12

Weight (kDa)

6.15

Isoelectric Point (pI)

49.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MMR_HSR1 PF01926 160 - 307 2.3e-23 50S ribosome-binding GTPase
FeoB_N PF02421 160 - 221 5.4e-09 Ferrous iron transport protein B
MMR_HSR1 PF01926 371 - 492 6.4e-27 50S ribosome-binding GTPase
FeoB_N PF02421 371 - 503 7.3e-10 Ferrous iron transport protein B
KH_dom-like PF14714 551 - 586 6.7e-06 KH-domain-like of EngA bacterial GTPase enzymes, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1561
AccB7I CCANNNNNTGG 1 cut(s) 1136
AccI GTMKAC 3 cut(s) 10, 633, 795
AccII CGCG 3 cut(s) 375, 911, 1740
AciI CCGC 5 cut(s) 167, 529, 877, 911, 1581
AclWI GGATC 3 cut(s) 591, 1286, 1422
AcoI YGGCCR 3 cut(s) 829, 1127, 1591
AcsI RAATTY 3 cut(s) 245, 959, 1229
AcuI CTGAAG 2 cut(s) 282, 1439
AfaI GTAC 3 cut(s) 884, 1660, 1823
AfiI CCNNNNNNNGG 9 cut(s) 405, 506, 538, 938, 1136, 1199, 1557, 1558, 1713
AflIII ACRYGT 1 cut(s) 1958
AgsI TTSAA 8 cut(s) 87, 526, 660, 1079, 1388, 1578, 1634, 1672
AjiI CACGTC 1 cut(s) 1961
AjnI CCWGG 3 cut(s) 568, 831, 1755
Alw21I GWGCWC 1 cut(s) 381
Alw26I GTCTC 4 cut(s) 375, 389, 972, 1499
AlwI GGATC 3 cut(s) 591, 1286, 1422
AlwNI CAGNNNCTG 3 cut(s) 791, 851, 1417
Ama87I CYCGRG 1 cut(s) 1058
AoxI GGCC 9 cut(s) 497, 829, 839, 1045, 1127, 1323, 1591, 1769, 1951
ApeKI GCWGC 9 cut(s) 197, 743, 788, 1024, 1311, 1511, 1619, 1622, 1760
ApoI RAATTY 3 cut(s) 245, 959, 1229
Asp700I GAANNNNTTC 1 cut(s) 720
AspS9I GGNCC 3 cut(s) 839, 1238, 1716
AsuHPI GGTGA 6 cut(s) 34, 96, 149, 623, 657, 925
AsuNHI GCTAGC 1 cut(s) 436
AvaI CYCGRG 1 cut(s) 1058
AvaII GGWCC 2 cut(s) 1238, 1716
BaeGI GKGCMC 1 cut(s) 1564
BalI TGGCCA 2 cut(s) 831, 1593
BanI GGYRCC 1 cut(s) 1561
BanII GRGCYC 1 cut(s) 381
BarI GAAGNNNNNNTAC 2 cut(s) 1642, 1674
BbsI GAAGAC 2 cut(s) 303, 1657
Bbv12I GWGCWC 1 cut(s) 381
BbvCI CCTCAGC 1 cut(s) 222
BbvI GCAGC 9 cut(s) 209, 755, 800, 1011, 1323, 1523, 1606, 1609, 1772
BccI CCATC 8 cut(s) 115, 139, 203, 314, 676, 821, 850, 1238
BceAI ACGGC 2 cut(s) 1677, 1884
BcgI CGANNNNNNTGC 4 cut(s) 158, 192, 239, 273
BciT130I CCWGG 3 cut(s) 570, 833, 1757
BciVI GTATCC 2 cut(s) 1474, 1810
BclI TGATCA 1 cut(s) 613
BcoDI GTCTC 4 cut(s) 375, 389, 972, 1499
BfaI CTAG 7 cut(s) 60, 77, 233, 437, 579, 735, 971
BfmI CTRYAG 5 cut(s) 486, 783, 1218, 1317, 1509
BfuI GTATCC 2 cut(s) 1474, 1810
BglII AGATCT 1 cut(s) 1907
BisI GCNGC 9 cut(s) 198, 744, 789, 1025, 1312, 1512, 1620, 1623, 1761
BlsI GCNGC 9 cut(s) 199, 745, 790, 1026, 1313, 1513, 1621, 1624, 1762
BmcAI AGTACT 1 cut(s) 884
Bme1390I CCNGG 3 cut(s) 570, 833, 1757
Bme18I GGWCC 2 cut(s) 1238, 1716
BmeT110I CYCGRG 1 cut(s) 1058
BmgBI CACGTC 1 cut(s) 1961
BmgT120I GGNCC 3 cut(s) 839, 1238, 1716
BmiI GGNNCC 3 cut(s) 214, 1563, 1718
BmrFI CCNGG 3 cut(s) 570, 833, 1757
BmsI GCATC 5 cut(s) 475, 747, 1204, 1213, 1846
BmtI GCTAGC 1 cut(s) 440
BpiI GAAGAC 2 cut(s) 303, 1657
BpmI CTGGAG 1 cut(s) 591
Bpu10I CCTNAGC 1 cut(s) 222
BpuEI CTTGAG 1 cut(s) 344
Bsa29I ATCGAT 2 cut(s) 309, 586
BsaAI YACGTR 2 cut(s) 935, 1821
BsaI GGTCTC 2 cut(s) 389, 972
BsaJI CCNNGG 3 cut(s) 1712, 1720, 1755
BsaWI WCCGGW 2 cut(s) 638, 1235
Bsc4I CCNNNNNNNGG 9 cut(s) 405, 506, 538, 938, 1136, 1199, 1557, 1558, 1713
Bse1I ACTGG 3 cut(s) 875, 1015, 1594
Bse3DI GCAATG 1 cut(s) 1110
BseBI CCWGG 3 cut(s) 570, 833, 1757
BseCI ATCGAT 2 cut(s) 309, 586
BseDI CCNNGG 3 cut(s) 1712, 1720, 1755
BseGI GGATG 7 cut(s) 131, 214, 295, 397, 568, 762, 1460
BseLI CCNNNNNNNGG 9 cut(s) 405, 506, 538, 938, 1136, 1199, 1557, 1558, 1713
BseMI GCAATG 1 cut(s) 1110
BseMII CTCAG 1 cut(s) 213
BseNI ACTGG 3 cut(s) 875, 1015, 1594
BseRI GAGGAG 4 cut(s) 63, 657, 1097, 1938
BseSI GKGCMC 1 cut(s) 1564
BseXI GCAGC 9 cut(s) 209, 755, 800, 1011, 1323, 1523, 1606, 1609, 1772
BseYI CCCAGC 2 cut(s) 1274, 1868
Bsh1236I CGCG 3 cut(s) 375, 911, 1740
BshFI GGCC 9 cut(s) 499, 831, 841, 1047, 1129, 1325, 1593, 1771, 1953
BshNI GGYRCC 1 cut(s) 1561
BshVI ATCGAT 2 cut(s) 309, 586
BsiHKAI GWGCWC 1 cut(s) 381
BsiHKCI CYCGRG 1 cut(s) 1058
BsiSI CCGG 2 cut(s) 639, 1236
BslFI GGGAC 1 cut(s) 1885
BslI CCNNNNNNNGG 9 cut(s) 405, 506, 538, 938, 1136, 1199, 1557, 1558, 1713
BsmAI GTCTC 4 cut(s) 375, 389, 972, 1499
BsmFI GGGAC 1 cut(s) 1885
BsmI GAATGC 1 cut(s) 169
BsnI GGCC 9 cut(s) 499, 831, 841, 1047, 1129, 1325, 1593, 1771, 1953
Bso31I GGTCTC 2 cut(s) 389, 972
BsoBI CYCGRG 1 cut(s) 1058
Bsp1286I GDGCHC 2 cut(s) 381, 1564
Bsp143I GATC 9 cut(s) 583, 599, 613, 1030, 1062, 1278, 1414, 1643, 1907
Bsp68I TCGCGA 1 cut(s) 375
BspACI CCGC 5 cut(s) 167, 529, 877, 911, 1581
BspANI GGCC 9 cut(s) 499, 831, 841, 1047, 1129, 1325, 1593, 1771, 1953
BspCNI CTCAG 1 cut(s) 214
BspDI ATCGAT 2 cut(s) 309, 586
BspFNI CGCG 3 cut(s) 375, 911, 1740
BspLI GGNNCC 3 cut(s) 214, 1563, 1718
BspMAI CTGCAG 1 cut(s) 1513
BspOI GCTAGC 1 cut(s) 440
BspPI GGATC 3 cut(s) 591, 1286, 1422
BspQI GCTCTTC 2 cut(s) 222, 678
BspT107I GGYRCC 1 cut(s) 1561
BspTNI GGTCTC 2 cut(s) 389, 972
BsrDI GCAATG 1 cut(s) 1110
BsrI ACTGG 3 cut(s) 875, 1015, 1594
BssECI CCNNGG 3 cut(s) 1712, 1720, 1755
BssMI GATC 9 cut(s) 583, 599, 613, 1030, 1062, 1278, 1414, 1643, 1907
BssT1I CCWWGG 1 cut(s) 1712
Bst2UI CCWGG 3 cut(s) 570, 833, 1757
Bst4CI ACNGT 2 cut(s) 655, 1630
Bst6I CTCTTC 4 cut(s) 121, 222, 678, 1074
BstAPI GCANNNNNTGC 2 cut(s) 749, 1865
BstBAI YACGTR 2 cut(s) 935, 1821
BstC8I GCNNGC 7 cut(s) 377, 438, 951, 1399, 1730, 1861, 1951
BstDEI CTNAG 3 cut(s) 222, 728, 1655
BstDSI CCRYGG 1 cut(s) 1720
BstF5I GGATG 7 cut(s) 131, 214, 295, 397, 568, 762, 1460
BstFNI CGCG 3 cut(s) 375, 911, 1740
BstKTI GATC 9 cut(s) 586, 602, 616, 1033, 1065, 1281, 1417, 1646, 1910
BstMAI GTCTC 4 cut(s) 375, 389, 972, 1499
BstMBI GATC 9 cut(s) 583, 599, 613, 1030, 1062, 1278, 1414, 1643, 1907
BstMWI GCNNNNNNNGC 7 cut(s) 749, 788, 1146, 1223, 1349, 1865, 1891
BstNI CCWGG 3 cut(s) 570, 833, 1757
BstNSI RCATGY 1 cut(s) 1401
BstSCI CCNGG 3 cut(s) 568, 831, 1755
BstSFI CTRYAG 5 cut(s) 486, 783, 1218, 1317, 1509
BstSLI GKGCMC 1 cut(s) 1564
BstSNI TACGTA 1 cut(s) 1821
BstUI CGCG 3 cut(s) 375, 911, 1740
BstV1I GCAGC 9 cut(s) 209, 755, 800, 1011, 1323, 1523, 1606, 1609, 1772
BstV2I GAAGAC 2 cut(s) 303, 1657
BstX2I RGATCY 2 cut(s) 1414, 1907
BstXI CCANNNNNNTGG 1 cut(s) 712
BstYI RGATCY 2 cut(s) 1414, 1907
Bsu15I ATCGAT 2 cut(s) 309, 586
BsuI GTATCC 2 cut(s) 1474, 1810
BsuRI GGCC 9 cut(s) 499, 831, 841, 1047, 1129, 1325, 1593, 1771, 1953
BsuTUI ATCGAT 2 cut(s) 309, 586
BtgI CCRYGG 1 cut(s) 1720
BtrI CACGTC 1 cut(s) 1961
BtsCI GGATG 7 cut(s) 131, 214, 295, 397, 568, 762, 1460
BtsIMutI CAGTG 2 cut(s) 1338, 1851
BtuMI TCGCGA 1 cut(s) 375
Cac8I GCNNGC 7 cut(s) 377, 438, 951, 1399, 1730, 1861, 1951
CaiI CAGNNNCTG 3 cut(s) 791, 851, 1417
Cfr13I GGNCC 3 cut(s) 839, 1238, 1716
ClaI ATCGAT 2 cut(s) 309, 586
CseI GACGC 1 cut(s) 1808
Csp6I GTAC 3 cut(s) 883, 1659, 1822
CviAII CATG 8 cut(s) 46, 679, 712, 766, 946, 1066, 1327, 1398
CviQI GTAC 3 cut(s) 883, 1659, 1822
DdeI CTNAG 3 cut(s) 222, 728, 1655
DpnI GATC 9 cut(s) 585, 601, 615, 1032, 1064, 1280, 1416, 1645, 1909
DpnII GATC 9 cut(s) 583, 599, 613, 1030, 1062, 1278, 1414, 1643, 1907
DraI TTTAAA 2 cut(s) 1155, 1702
EaeI YGGCCR 3 cut(s) 829, 1127, 1591
Eam1104I CTCTTC 4 cut(s) 121, 222, 678, 1074
EarI CTCTTC 4 cut(s) 121, 222, 678, 1074
Ecl136II GAGCTC 1 cut(s) 379
Eco105I TACGTA 1 cut(s) 1821
Eco130I CCWWGG 1 cut(s) 1712
Eco147I AGGCCT 2 cut(s) 499, 1953
Eco24I GRGCYC 1 cut(s) 381
Eco31I GGTCTC 2 cut(s) 389, 972
Eco32I GATATC 2 cut(s) 313, 343
Eco47I GGWCC 2 cut(s) 1238, 1716
Eco53kI GAGCTC 1 cut(s) 379
Eco57I CTGAAG 2 cut(s) 282, 1439
Eco88I CYCGRG 1 cut(s) 1058
EcoICRI GAGCTC 1 cut(s) 379
EcoO109I RGGNCCY 1 cut(s) 1716
EcoRI GAATTC 1 cut(s) 245
EcoRII CCWGG 3 cut(s) 568, 831, 1755
EcoRV GATATC 2 cut(s) 313, 343
EcoT14I CCWWGG 1 cut(s) 1712
EcoT22I ATGCAT 1 cut(s) 1403
EcoT38I GRGCYC 1 cut(s) 381
ErhI CCWWGG 1 cut(s) 1712
FaeI CATG 8 cut(s) 49, 682, 715, 769, 949, 1069, 1330, 1401
FalI AAGNNNNNCTT 2 cut(s) 745, 777
FaqI GGGAC 1 cut(s) 1885
FatI CATG 8 cut(s) 45, 678, 711, 765, 945, 1065, 1326, 1397
FauI CCCGC 1 cut(s) 174
FbaI TGATCA 1 cut(s) 613
FblI GTMKAC 3 cut(s) 10, 633, 795
Fnu4HI GCNGC 9 cut(s) 198, 744, 789, 1025, 1312, 1512, 1620, 1623, 1761
FokI GGATG 7 cut(s) 118, 221, 302, 404, 575, 769, 1467
FriOI GRGCYC 1 cut(s) 381
Fsp4HI GCNGC 9 cut(s) 198, 744, 789, 1025, 1312, 1512, 1620, 1623, 1761
FspBI CTAG 7 cut(s) 60, 77, 233, 437, 579, 735, 971
GluI GCNGC 9 cut(s) 198, 744, 789, 1025, 1312, 1512, 1620, 1623, 1761
GsaI CCCAGC 2 cut(s) 1278, 1872
GsuI CTGGAG 1 cut(s) 591
HaeIII GGCC 9 cut(s) 499, 831, 841, 1047, 1129, 1325, 1593, 1771, 1953
HapII CCGG 2 cut(s) 639, 1236
HgaI GACGC 1 cut(s) 1808
Hin1II CATG 8 cut(s) 49, 682, 715, 769, 949, 1069, 1330, 1401
HincII GTYRAC 4 cut(s) 24, 699, 796, 1603
HindII GTYRAC 4 cut(s) 24, 699, 796, 1603
HinfI GANTC 8 cut(s) 7, 17, 302, 800, 926, 942, 1336, 1873
HpaII CCGG 2 cut(s) 639, 1236
HphI GGTGA 6 cut(s) 34, 96, 149, 623, 657, 925
Hpy188I TCNGA 9 cut(s) 289, 509, 958, 1169, 1366, 1419, 1816, 1912, 1965
Hpy99I CGWCG 4 cut(s) 800, 1362, 1780, 1802
HpyAV CCTTC 6 cut(s) 403, 489, 710, 771, 1439, 1821
HpyCH4III ACNGT 2 cut(s) 655, 1630
HpyCH4IV ACGT 4 cut(s) 934, 1778, 1820, 1960
HpyF10VI GCNNNNNNNGC 7 cut(s) 749, 788, 1146, 1223, 1349, 1865, 1891
HpyF3I CTNAG 3 cut(s) 222, 728, 1655
HpySE526I ACGT 4 cut(s) 934, 1778, 1820, 1960
Hsp92II CATG 8 cut(s) 49, 682, 715, 769, 949, 1069, 1330, 1401
Ksp22I TGATCA 1 cut(s) 613
Kzo9I GATC 9 cut(s) 583, 599, 613, 1030, 1062, 1278, 1414, 1643, 1907
LguI GCTCTTC 2 cut(s) 222, 678
LmnI GCTCC 1 cut(s) 1548
Lsp1109I GCAGC 9 cut(s) 209, 755, 800, 1011, 1323, 1523, 1606, 1609, 1772
LweI GCATC 5 cut(s) 475, 747, 1204, 1213, 1846
MaeI CTAG 7 cut(s) 60, 77, 233, 437, 579, 735, 971
MaeII ACGT 4 cut(s) 934, 1778, 1820, 1960
MaeIII GTNAC 2 cut(s) 574, 663
MalI GATC 9 cut(s) 585, 601, 615, 1032, 1064, 1280, 1416, 1645, 1909
MboI GATC 9 cut(s) 583, 599, 613, 1030, 1062, 1278, 1414, 1643, 1907
MfeI CAATTG 1 cut(s) 1182
MflI RGATCY 2 cut(s) 1414, 1907
MhlI GDGCHC 2 cut(s) 381, 1564
MlsI TGGCCA 2 cut(s) 831, 1593
MluNI TGGCCA 2 cut(s) 831, 1593
MlyI GAGTC 4 cut(s) 16, 296, 936, 1867
MmeI TCCRAC 2 cut(s) 472, 487
Mox20I TGGCCA 2 cut(s) 831, 1593
Mph1103I ATGCAT 1 cut(s) 1403
MroXI GAANNNNTTC 1 cut(s) 720
MscI TGGCCA 2 cut(s) 831, 1593
MseI TTAA 3 cut(s) 1053, 1154, 1701
MslI CAYNNNNRTG 2 cut(s) 710, 1331
Msp20I TGGCCA 2 cut(s) 831, 1593
MspA1I CMGCKG 3 cut(s) 791, 806, 1274
MspI CCGG 2 cut(s) 639, 1236
MspR9I CCNGG 3 cut(s) 570, 833, 1757
MunI CAATTG 1 cut(s) 1182
Mva1269I GAATGC 1 cut(s) 169
MvaI CCWGG 3 cut(s) 570, 833, 1757
MvnI CGCG 3 cut(s) 375, 911, 1740
MwoI GCNNNNNNNGC 7 cut(s) 749, 788, 1146, 1223, 1349, 1865, 1891
NdeII GATC 9 cut(s) 583, 599, 613, 1030, 1062, 1278, 1414, 1643, 1907
NheI GCTAGC 1 cut(s) 436
NlaIII CATG 8 cut(s) 49, 682, 715, 769, 949, 1069, 1330, 1401
NlaIV GGNNCC 3 cut(s) 214, 1563, 1718
NmuCI GTSAC 1 cut(s) 663
NruI TCGCGA 1 cut(s) 375
NsiI ATGCAT 1 cut(s) 1403
NspI RCATGY 1 cut(s) 1401
PaeI GCATGC 1 cut(s) 1401
PaeR7I CTCGAG 1 cut(s) 1058
PceI AGGCCT 2 cut(s) 499, 1953
PciSI GCTCTTC 2 cut(s) 222, 678
PcsI WCGNNNNNNNCGW 5 cut(s) 246, 306, 315, 1345, 1354
PctI GAATGC 1 cut(s) 169
PdmI GAANNNNTTC 1 cut(s) 720
PfeI GAWTC 4 cut(s) 17, 800, 926, 1336
PflMI CCANNNNNTGG 1 cut(s) 1136
PkrI GCNGC 9 cut(s) 199, 745, 790, 1026, 1313, 1513, 1621, 1624, 1762
PleI GAGTC 4 cut(s) 15, 296, 936, 1867
PpsI GAGTC 4 cut(s) 15, 296, 936, 1867
Ppu21I YACGTR 2 cut(s) 935, 1821
PpuMI RGGWCCY 1 cut(s) 1716
Psp124BI GAGCTC 1 cut(s) 381
Psp5II RGGWCCY 1 cut(s) 1716
Psp6I CCWGG 3 cut(s) 568, 831, 1755
PspFI CCCAGC 2 cut(s) 1274, 1868
PspGI CCWGG 3 cut(s) 568, 831, 1755
PspN4I GGNNCC 3 cut(s) 214, 1563, 1718
PspPI GGNCC 3 cut(s) 839, 1238, 1716
PspPPI RGGWCCY 1 cut(s) 1716
PstI CTGCAG 1 cut(s) 1513
PstNI CAGNNNCTG 3 cut(s) 791, 851, 1417
PsuI RGATCY 2 cut(s) 1414, 1907
PvuII CAGCTG 3 cut(s) 791, 806, 1274
RruI TCGCGA 1 cut(s) 375
RsaI GTAC 3 cut(s) 884, 1660, 1823
RsaNI GTAC 3 cut(s) 883, 1659, 1822
RseI CAYNNNNRTG 2 cut(s) 710, 1331
SacI GAGCTC 1 cut(s) 381
SalI GTCGAC 1 cut(s) 794
SapI GCTCTTC 2 cut(s) 222, 678
SaqAI TTAA 3 cut(s) 1053, 1154, 1701
SatI GCNGC 9 cut(s) 198, 744, 789, 1025, 1312, 1512, 1620, 1623, 1761
Sau3AI GATC 9 cut(s) 583, 599, 613, 1030, 1062, 1278, 1414, 1643, 1907
Sau96I GGNCC 3 cut(s) 839, 1238, 1716
ScaI AGTACT 1 cut(s) 884
SchI GAGTC 4 cut(s) 16, 296, 936, 1867
ScrFI CCNGG 3 cut(s) 570, 833, 1757
SduI GDGCHC 2 cut(s) 381, 1564
SfaNI GCATC 5 cut(s) 475, 747, 1204, 1213, 1846
SfcI CTRYAG 5 cut(s) 486, 783, 1218, 1317, 1509
Sfr274I CTCGAG 1 cut(s) 1058
SinI GGWCC 2 cut(s) 1238, 1716
SlaI CTCGAG 1 cut(s) 1058
SmiI ATTTAAAT 1 cut(s) 1702
SmiMI CAYNNNNRTG 2 cut(s) 710, 1331
SmlI CTYRAG 2 cut(s) 323, 1058
SmoI CTYRAG 2 cut(s) 323, 1058
SnaBI TACGTA 1 cut(s) 1821
SphI GCATGC 1 cut(s) 1401
SseBI AGGCCT 2 cut(s) 499, 1953
SsiI CCGC 5 cut(s) 167, 529, 877, 911, 1581
SspI AATATT 1 cut(s) 1609
SspMI CTAG 7 cut(s) 60, 77, 233, 437, 579, 735, 971
SstI GAGCTC 1 cut(s) 381
StuI AGGCCT 2 cut(s) 499, 1953
StyD4I CCNGG 3 cut(s) 568, 831, 1755
StyI CCWWGG 1 cut(s) 1712
SwaI ATTTAAAT 1 cut(s) 1702
TaaI ACNGT 2 cut(s) 655, 1630
TaiI ACGT 4 cut(s) 937, 1781, 1823, 1963
TatI WGTACW 1 cut(s) 882
TfiI GAWTC 4 cut(s) 17, 800, 926, 1336
Tru1I TTAA 3 cut(s) 1053, 1154, 1701
Tru9I TTAA 3 cut(s) 1053, 1154, 1701
TscAI CASTG 2 cut(s) 1338, 1858
TseFI GTSAC 1 cut(s) 663
TseI GCWGC 9 cut(s) 197, 743, 788, 1024, 1311, 1511, 1619, 1622, 1760
Tsp45I GTSAC 1 cut(s) 663
TspDTI ATGAA 3 cut(s) 81, 579, 1293
TspGWI ACGGA 2 cut(s) 307, 1535
TspRI CASTG 2 cut(s) 1338, 1858
Van91I CCANNNNNTGG 1 cut(s) 1136
VpaK11BI GGWCC 2 cut(s) 1238, 1716
XapI RAATTY 3 cut(s) 245, 959, 1229
XceI RCATGY 1 cut(s) 1401
XcmI CCANNNNNNNNNTGG 1 cut(s) 709
XhoI CTCGAG 1 cut(s) 1058
XmiI GTMKAC 3 cut(s) 10, 633, 795
XmnI GAANNNNTTC 1 cut(s) 720
XspI CTAG 7 cut(s) 60, 77, 233, 437, 579, 735, 971
ZrmI AGTACT 1 cut(s) 884
Zsp2I ATGCAT 1 cut(s) 1403
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.