MD03G1176500.v1.1

KH-domain-like of EngA bacterial GTPase enzymes, C-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0001492
Physical Location & Seq
Forward (+)
24216219 .. 24223717
7499 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1176500.v1.1.491

Sequence Viewer

Length: 1983 bp
ATGGCAGCCCCCTCGGAGCTCTTGTGCTACAGAGGAACCACCACTTCTCCCTCTCTCTCCAAAACTCTCTCTCTCCTCTCTTCCCCATTTCCAATTCCCAAATATTTCTCTCGCGGTCTCCGCACTCCTTCCCTTTCTCTCCACACTTCTCTTCACACTCTTCCTTGCTTCGTTTCCCGCCTCCCTGCCAACGAATTCAATATCTCCTCCACAGCAGATGACCAACAACAACAAGAAGGCGATGGCGAAGAGGAGGAGGAGCTCGTTGACGAGTCCGACGGCGGCGGCGGCGAGTTATTGACGGCCGACGTCGACGACCTGGAAGAAGACGCTCGATACTTAGTTGAAGAGTTCTCTTCCTCTTTGTCCACCCAACTCAGAATTGAAGATGACAAAGATGACCAAGAAAAAGTTGGTCGAAAGCAGAGGAATCAGAAGAGTACAGTTAAAACTATCCCTGACCATCTTCTTCCAAGGGTTGCAATTGTTGGAAGACCGAATGTTGGCAAATCAGCATTATTTAATCGTCTTGTCTCGGGGAACAAGGCAATTGTAGTGGATGAGCCAGGGGTTACTAGGGATCGCCTGTATGGTAGATCCTTTTGGGGTGACCATGAATTTATGGTGGTGGATACTGGTGGTGTACTTACAGTTTCAAAGTCCCAGGCTAATGTCATGGAAGAATTAGCTATTTCAACAACCATTGGTATGGATGGAATCCCACTTGCCTCAAGGGAGGCAGCTGTTGCTAGGATGCCCTCAATGATAGAGAAACAAGCTACTGCAGCTGTTGAAGAATCATCTGTCATTATATTCCTCGTTGATGGCCAGGCAGGTCTAACTGCAGCAGATGAGGAAATTTCAGATTGGCTACGTAAAAACTTCTCAGATAAGTACATCATTCTTGCAGTTAACAAGTGTGAATCTCCACGTAAAGGGATCATGCAAGCATCAGAATTTTGGTCATTGGGGTTTTCACCACTTCCTATATCTGCTATATCTGGAACTGGAACTGGAGAGCTTCTTGATCTTGTGTGTTCAGGGCTGAAAAAGAATGAGGACCCGGAGGATTTTGCTGAACAGGAAGAGTATGTTCCTGCAATTGCAATTGTTGGCCGACCAAATGTTGGTAAAAGTAGCATTTTGAATGCATTGGTTGGAGAGGACAGGACAATTGTTAGCCCAGTCAGTGGAACAACCCGTGATGCTATTGATATGGAATTTACTGGACCAGATGGACAGAAATTCAAACTAATTGATACTGCTGGAATCAGAAGAAGAGCCGTTGTTGCTTCATCAGGTAGCATGACAGAGGCTTTATCTGTAAATCGAGCATTTCGTGCCATTCGTCGTTCTGATGTTGTGGCTCTTGTCATTGAGGCCCTGGCTTGTATCACAGAACAGGATTGCAAGATTGCTGACAGGATAGAAAGAGAAGGCAAAGGTTGCCTGATAGTTGTAAATAAATGGGATACCATACCAAATAAAAACCAGCAGACTGCGACATACTATGAGCAGGATGTTAGGGAGAAGCTTCGTATCCTTGGCTGGGCACCTATTGTATATTCAACTGCAATAGCTGGTCAGAGTGTTGATAAAATTATTGATGCTGCTAGCACAGTTGAGAAAGAAAGATCAAGAAGGCTTAGTACTTCTGTAGTAAACCAAGTGGTCCAGGAAGCACTAGCTTTTAAATCACCACCGAGGACACGAGGTGGCAAAAGAGGCCGCATTTATTACTGCACTCAGGCAGCTATACGGCCACCAACATTTGTGTTCTTTGTCGATGATTCAAAACTTTTCCCCGAGACATACCGGCGTTATATGGAGAAGCAACTCCGTTCAGATGCTGGGTTTTTAGGCACTCCAATTAGGCTTCTATGGTCTAACAGGAGAAAAACAGAAAAAGAGGAAGGAAAAGCTGCAACGCGAGCACAGGCAAATCTTGTGCCACGTGACCGAAAATTGGAGTTAGCTACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KH_dom-like MMR_HSR1

Protein Analysis

661

Amino Acids

72.61

Weight (kDa)

5.41

Isoelectric Point (pI)

46.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MMR_HSR1 PF01926 159 - 306 2e-23 50S ribosome-binding GTPase
FeoB_N PF02421 159 - 221 2.8e-08 Ferrous iron transport protein B
MMR_HSR1 PF01926 368 - 489 1.2e-26 50S ribosome-binding GTPase
FeoB_N PF02421 368 - 536 3.1e-09 Ferrous iron transport protein B
KH_dom-like PF14714 548 - 628 1.2e-27 KH-domain-like of EngA bacterial GTPase enzymes, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 312
Acc36I ACCTGC 1 cut(s) 824
AccB1I GGYRCC 1 cut(s) 1552
AccB7I CCANNNNNTGG 2 cut(s) 1127, 1190
AccI GTMKAC 1 cut(s) 312
AccII CGCG 2 cut(s) 114, 1930
AciI CCGC 7 cut(s) 114, 121, 178, 282, 285, 288, 1729
AclWI GGATC 3 cut(s) 588, 591, 947
AcoI YGGCCR 4 cut(s) 303, 826, 1114, 1760
AcsI RAATTY 6 cut(s) 194, 617, 858, 956, 1220, 1244
AcvI CACGTG 1 cut(s) 1955
AcyI GRCGYC 1 cut(s) 309
AdeI CACNNNGTG 1 cut(s) 1715
AfaI GTAC 4 cut(s) 442, 645, 896, 1651
AfiI CCNNNNNNNGG 6 cut(s) 503, 935, 1127, 1190, 1549, 1966
AjnI CCWGG 6 cut(s) 318, 565, 663, 828, 1383, 1674
Alw21I GWGCWC 3 cut(s) 21, 264, 1936
Alw26I GTCTC 3 cut(s) 122, 538, 1802
AlwI GGATC 3 cut(s) 588, 591, 947
AlwNI CAGNNNCTG 1 cut(s) 1850
Ama87I CYCGRG 2 cut(s) 535, 1805
AoxI GGCC 6 cut(s) 303, 826, 1114, 1380, 1726, 1760
ApeKI GCWGC 7 cut(s) 5, 740, 785, 845, 1610, 1751, 1922
ApoI RAATTY 6 cut(s) 194, 617, 858, 956, 1220, 1244
AspS9I GGNCC 4 cut(s) 1060, 1229, 1381, 1672
AsuC2I CCSGG 1 cut(s) 1064
AsuHPI GGTGA 3 cut(s) 620, 969, 1689
AsuNHI GCTAGC 1 cut(s) 1613
AvaI CYCGRG 2 cut(s) 535, 1805
AvaII GGWCC 3 cut(s) 1060, 1229, 1672
BaeGI GKGCMC 1 cut(s) 1555
BalI TGGCCA 1 cut(s) 828
BanI GGYRCC 1 cut(s) 1552
BanII GRGCYC 2 cut(s) 21, 264
BarI GAAGNNNNNNTAC 2 cut(s) 1633, 1665
BauI CACGAG 1 cut(s) 1710
BbrPI CACGTG 1 cut(s) 1955
BbsI GAAGAC 2 cut(s) 333, 499
Bbv12I GWGCWC 3 cut(s) 21, 264, 1936
BbvI GCAGC 7 cut(s) 17, 752, 797, 857, 1597, 1763, 1909
BccI CCATC 5 cut(s) 236, 471, 707, 818, 1229
BceAI ACGGC 4 cut(s) 295, 318, 1268, 1775
BcgI CGANNNNNNTGC 1 cut(s) 28
BciT130I CCWGG 6 cut(s) 320, 567, 665, 830, 1385, 1676
BciVI GTATCC 3 cut(s) 625, 1465, 1550
BcnI CCSGG 1 cut(s) 1064
BcoDI GTCTC 3 cut(s) 122, 538, 1802
BfaI CTAG 4 cut(s) 576, 750, 1614, 1685
BfmI CTRYAG 4 cut(s) 28, 783, 843, 1656
BfuAI ACCTGC 1 cut(s) 824
BfuI GTATCC 3 cut(s) 625, 1465, 1550
BmcAI AGTACT 1 cut(s) 1651
Bme1390I CCNGG 7 cut(s) 320, 567, 665, 830, 1064, 1385, 1676
Bme18I GGWCC 3 cut(s) 1060, 1229, 1672
BmeT110I CYCGRG 2 cut(s) 535, 1805
BmgT120I GGNCC 4 cut(s) 1060, 1229, 1381, 1672
BmiI GGNNCC 3 cut(s) 37, 1062, 1554
BmrFI CCNGG 7 cut(s) 320, 567, 665, 830, 1064, 1385, 1676
BmrI ACTGGG 1 cut(s) 1178
BmsI GCATC 5 cut(s) 744, 959, 1195, 1597, 1837
BmtI GCTAGC 1 cut(s) 1617
BmuI ACTGGG 1 cut(s) 1178
BpiI GAAGAC 2 cut(s) 333, 499
BpmI CTGGAG 1 cut(s) 1035
BpuEI CTTGAG 1 cut(s) 715
BpuMI CCSGG 1 cut(s) 1064
BsaAI YACGTR 3 cut(s) 875, 932, 1955
BsaHI GRCGYC 1 cut(s) 309
BsaI GGTCTC 1 cut(s) 122
BsaJI CCNNGG 7 cut(s) 12, 473, 566, 663, 1383, 1543, 1703
BsaXI ACNNNNNCTCC 4 cut(s) 8, 31, 38, 61
Bsc4I CCNNNNNNNGG 6 cut(s) 503, 935, 1127, 1190, 1549, 1966
Bse118I RCCGGY 1 cut(s) 1815
Bse1I ACTGG 5 cut(s) 640, 1012, 1018, 1184, 1231
BseBI CCWGG 6 cut(s) 320, 567, 665, 830, 1385, 1676
BseDI CCNNGG 7 cut(s) 12, 473, 566, 663, 1383, 1543, 1703
BseGI GGATG 4 cut(s) 565, 718, 759, 1525
BseLI CCNNNNNNNGG 6 cut(s) 503, 935, 1127, 1190, 1549, 1966
BseMII CTCAG 3 cut(s) 391, 900, 1760
BseNI ACTGG 5 cut(s) 640, 1012, 1018, 1184, 1231
BseRI GAGGAG 5 cut(s) 65, 196, 266, 269, 272
BseSI GKGCMC 1 cut(s) 1555
BseX3I CGGCCG 1 cut(s) 303
BseXI GCAGC 7 cut(s) 17, 752, 797, 857, 1597, 1763, 1909
BseYI CCCAGC 2 cut(s) 1548, 1850
BsgI GTGCAG 1 cut(s) 1726
Bsh1236I CGCG 2 cut(s) 114, 1930
Bsh1285I CGRYCG 1 cut(s) 306
BshFI GGCC 6 cut(s) 305, 828, 1116, 1382, 1728, 1762
BshNI GGYRCC 1 cut(s) 1552
BsiEI CGRYCG 1 cut(s) 306
BsiHKAI GWGCWC 3 cut(s) 21, 264, 1936
BsiHKCI CYCGRG 2 cut(s) 535, 1805
BsiSI CCGG 2 cut(s) 1064, 1816
BslFI GGGAC 1 cut(s) 646
BslI CCNNNNNNNGG 6 cut(s) 503, 935, 1127, 1190, 1549, 1966
BsmAI GTCTC 3 cut(s) 122, 538, 1802
BsmFI GGGAC 1 cut(s) 646
BsmI GAATGC 1 cut(s) 1153
BsnI GGCC 6 cut(s) 305, 828, 1116, 1382, 1728, 1762
Bso31I GGTCTC 1 cut(s) 122
BsoBI CYCGRG 2 cut(s) 535, 1805
Bsp1286I GDGCHC 4 cut(s) 21, 264, 1555, 1936
Bsp143I GATC 5 cut(s) 580, 596, 939, 1027, 1634
BspACI CCGC 7 cut(s) 114, 121, 178, 282, 285, 288, 1729
BspANI GGCC 6 cut(s) 305, 828, 1116, 1382, 1728, 1762
BspCNI CTCAG 3 cut(s) 390, 899, 1759
BspFNI CGCG 2 cut(s) 114, 1930
BspLI GGNNCC 3 cut(s) 37, 1062, 1554
BspMAI CTGCAG 2 cut(s) 787, 847
BspMI ACCTGC 1 cut(s) 824
BspOI GCTAGC 1 cut(s) 1617
BspPI GGATC 3 cut(s) 588, 591, 947
BspQI GCTCTTC 1 cut(s) 1273
BspT107I GGYRCC 1 cut(s) 1552
BspTNI GGTCTC 1 cut(s) 122
BsrFI RCCGGY 1 cut(s) 1815
BsrI ACTGG 5 cut(s) 640, 1012, 1018, 1184, 1231
BssAI RCCGGY 1 cut(s) 1815
BssECI CCNNGG 7 cut(s) 12, 473, 566, 663, 1383, 1543, 1703
BssMI GATC 5 cut(s) 580, 596, 939, 1027, 1634
BssNI GRCGYC 1 cut(s) 309
BssSI CACGAG 1 cut(s) 1710
BssT1I CCWWGG 2 cut(s) 473, 1543
Bst2BI CACGAG 1 cut(s) 1710
Bst2UI CCWGG 6 cut(s) 320, 567, 665, 830, 1385, 1676
Bst4CI ACNGT 3 cut(s) 445, 652, 1621
Bst6I CTCTTC 9 cut(s) 85, 156, 165, 243, 342, 361, 431, 1080, 1273
BstACI GRCGYC 1 cut(s) 309
BstAPI GCANNNNNTGC 3 cut(s) 746, 1340, 1446
BstBAI YACGTR 3 cut(s) 875, 932, 1955
BstC8I GCNNGC 3 cut(s) 948, 1615, 1932
BstDEI CTNAG 5 cut(s) 340, 377, 886, 1646, 1746
BstEII GGTNACC 1 cut(s) 608
BstF5I GGATG 4 cut(s) 565, 718, 759, 1525
BstFNI CGCG 2 cut(s) 114, 1930
BstKTI GATC 5 cut(s) 583, 599, 942, 1030, 1637
BstMAI GTCTC 3 cut(s) 122, 538, 1802
BstMBI GATC 5 cut(s) 580, 596, 939, 1027, 1634
BstMCI CGRYCG 1 cut(s) 306
BstMWI GCNNNNNNNGC 9 cut(s) 120, 288, 746, 785, 1289, 1340, 1446, 1725, 1931
BstNI CCWGG 6 cut(s) 320, 567, 665, 830, 1385, 1676
BstPI GGTNACC 1 cut(s) 608
BstSCI CCNGG 7 cut(s) 318, 565, 663, 828, 1062, 1383, 1674
BstSFI CTRYAG 4 cut(s) 28, 783, 843, 1656
BstSLI GKGCMC 1 cut(s) 1555
BstSNI TACGTA 1 cut(s) 875
BstUI CGCG 2 cut(s) 114, 1930
BstV1I GCAGC 7 cut(s) 17, 752, 797, 857, 1597, 1763, 1909
BstV2I GAAGAC 2 cut(s) 333, 499
BstX2I RGATCY 1 cut(s) 596
BstXI CCANNNNNNTGG 1 cut(s) 709
BstYI RGATCY 1 cut(s) 596
BstZI CGGCCG 1 cut(s) 303
BsuI GTATCC 3 cut(s) 625, 1465, 1550
BsuRI GGCC 6 cut(s) 305, 828, 1116, 1382, 1728, 1762
BtgZI GCGATG 1 cut(s) 255
BtsCI GGATG 4 cut(s) 565, 718, 759, 1525
BtsIMutI CAGTG 1 cut(s) 1195
BveI ACCTGC 1 cut(s) 824
Cac8I GCNNGC 3 cut(s) 948, 1615, 1932
CaiI CAGNNNCTG 1 cut(s) 1850
Cfr10I RCCGGY 1 cut(s) 1815
Cfr13I GGNCC 4 cut(s) 1060, 1229, 1381, 1672
CseI GACGC 1 cut(s) 338
Csp6I GTAC 4 cut(s) 441, 644, 895, 1650
CspCI CAANNNNNGTGG 2 cut(s) 537, 572
CviAII CATG 5 cut(s) 614, 676, 943, 1306, 1980
CviQI GTAC 4 cut(s) 441, 644, 895, 1650
DdeI CTNAG 5 cut(s) 340, 377, 886, 1646, 1746
DpnI GATC 5 cut(s) 582, 598, 941, 1029, 1636
DpnII GATC 5 cut(s) 580, 596, 939, 1027, 1634
DraI TTTAAA 1 cut(s) 1693
DraIII CACNNNGTG 1 cut(s) 1715
EaeI YGGCCR 4 cut(s) 303, 826, 1114, 1760
EagI CGGCCG 1 cut(s) 303
Eam1104I CTCTTC 9 cut(s) 85, 156, 165, 243, 342, 361, 431, 1080, 1273
EarI CTCTTC 9 cut(s) 85, 156, 165, 243, 342, 361, 431, 1080, 1273
Ecl136II GAGCTC 2 cut(s) 19, 262
EclXI CGGCCG 1 cut(s) 303
Eco105I TACGTA 1 cut(s) 875
Eco130I CCWWGG 2 cut(s) 473, 1543
Eco24I GRGCYC 2 cut(s) 21, 264
Eco31I GGTCTC 1 cut(s) 122
Eco47I GGWCC 3 cut(s) 1060, 1229, 1672
Eco52I CGGCCG 1 cut(s) 303
Eco53kI GAGCTC 2 cut(s) 19, 262
Eco72I CACGTG 1 cut(s) 1955
Eco88I CYCGRG 2 cut(s) 535, 1805
Eco91I GGTNACC 1 cut(s) 608
EcoICRI GAGCTC 2 cut(s) 19, 262
EcoO109I RGGNCCY 2 cut(s) 1060, 1381
EcoO65I GGTNACC 1 cut(s) 608
EcoRI GAATTC 1 cut(s) 194
EcoRII CCWGG 6 cut(s) 318, 565, 663, 828, 1383, 1674
EcoT14I CCWWGG 2 cut(s) 473, 1543
EcoT22I ATGCAT 1 cut(s) 1153
EcoT38I GRGCYC 2 cut(s) 21, 264
ErhI CCWWGG 2 cut(s) 473, 1543
FaeI CATG 5 cut(s) 617, 679, 946, 1309, 1983
FaqI GGGAC 1 cut(s) 646
FatI CATG 5 cut(s) 613, 675, 942, 1305, 1979
FauI CCCGC 1 cut(s) 185
FblI GTMKAC 1 cut(s) 312
FokI GGATG 4 cut(s) 572, 725, 766, 1532
FriOI GRGCYC 2 cut(s) 21, 264
FspBI CTAG 4 cut(s) 576, 750, 1614, 1685
GsaI CCCAGC 2 cut(s) 1552, 1854
GsuI CTGGAG 1 cut(s) 1035
HaeIII GGCC 6 cut(s) 305, 828, 1116, 1382, 1728, 1762
HapII CCGG 2 cut(s) 1064, 1816
HgaI GACGC 1 cut(s) 338
Hin1I GRCGYC 1 cut(s) 309
Hin1II CATG 5 cut(s) 617, 679, 946, 1309, 1983
HincII GTYRAC 3 cut(s) 268, 313, 913
HindII GTYRAC 3 cut(s) 268, 313, 913
HindIII AAGCTT 1 cut(s) 1532
HinfI GANTC 7 cut(s) 272, 430, 717, 797, 923, 1269, 1790
HpaI GTTAAC 1 cut(s) 913
HpaII CCGG 2 cut(s) 1064, 1816
HphI GGTGA 3 cut(s) 620, 969, 1689
Hpy166II GTNNAC 6 cut(s) 268, 313, 369, 644, 913, 1663
Hpy188III TCNNGA 3 cut(s) 1002, 1025, 1638
Hpy8I GTNNAC 6 cut(s) 268, 313, 369, 644, 913, 1663
Hpy99I CGWCG 5 cut(s) 281, 311, 314, 317, 1353
HpyAV CCTTC 5 cut(s) 138, 230, 1430, 1635, 1907
HpyCH4III ACNGT 3 cut(s) 445, 652, 1621
HpyCH4IV ACGT 4 cut(s) 309, 874, 931, 1954
HpyF10VI GCNNNNNNNGC 9 cut(s) 120, 288, 746, 785, 1289, 1340, 1446, 1725, 1931
HpyF3I CTNAG 5 cut(s) 340, 377, 886, 1646, 1746
HpySE526I ACGT 4 cut(s) 309, 874, 931, 1954
Hsp92I GRCGYC 1 cut(s) 309
Hsp92II CATG 5 cut(s) 617, 679, 946, 1309, 1983
KspAI GTTAAC 1 cut(s) 913
Kzo9I GATC 5 cut(s) 580, 596, 939, 1027, 1634
LguI GCTCTTC 1 cut(s) 1273
LmnI GCTCC 2 cut(s) 16, 259
Lsp1109I GCAGC 7 cut(s) 17, 752, 797, 857, 1597, 1763, 1909
LweI GCATC 5 cut(s) 744, 959, 1195, 1597, 1837
MaeI CTAG 4 cut(s) 576, 750, 1614, 1685
MaeII ACGT 4 cut(s) 309, 874, 931, 1954
MaeIII GTNAC 3 cut(s) 571, 608, 1955
MalI GATC 5 cut(s) 582, 598, 941, 1029, 1636
MboI GATC 5 cut(s) 580, 596, 939, 1027, 1634
MfeI CAATTG 5 cut(s) 483, 549, 1101, 1107, 1173
MflI RGATCY 1 cut(s) 596
MhlI GDGCHC 4 cut(s) 21, 264, 1555, 1936
MlsI TGGCCA 1 cut(s) 828
MluNI TGGCCA 1 cut(s) 828
MlyI GAGTC 1 cut(s) 281
MmeI TCCRAC 3 cut(s) 300, 469, 1138
Mox20I TGGCCA 1 cut(s) 828
Mph1103I ATGCAT 1 cut(s) 1153
MscI TGGCCA 1 cut(s) 828
MseI TTAA 4 cut(s) 447, 522, 912, 1692
MslI CAYNNNNRTG 1 cut(s) 707
Msp20I TGGCCA 1 cut(s) 828
MspA1I CMGCKG 2 cut(s) 743, 788
MspI CCGG 2 cut(s) 1064, 1816
MspR9I CCNGG 7 cut(s) 320, 567, 665, 830, 1064, 1385, 1676
MunI CAATTG 5 cut(s) 483, 549, 1101, 1107, 1173
Mva1269I GAATGC 1 cut(s) 1153
MvaI CCWGG 6 cut(s) 320, 567, 665, 830, 1385, 1676
MvnI CGCG 2 cut(s) 114, 1930
MwoI GCNNNNNNNGC 9 cut(s) 120, 288, 746, 785, 1289, 1340, 1446, 1725, 1931
NciI CCSGG 1 cut(s) 1064
NdeII GATC 5 cut(s) 580, 596, 939, 1027, 1634
NheI GCTAGC 1 cut(s) 1613
NlaIII CATG 5 cut(s) 617, 679, 946, 1309, 1983
NlaIV GGNNCC 3 cut(s) 37, 1062, 1554
NmuCI GTSAC 2 cut(s) 608, 1955
NsiI ATGCAT 1 cut(s) 1153
PciSI GCTCTTC 1 cut(s) 1273
PcsI WCGNNNNNNNCGW 2 cut(s) 1336, 1345
PctI GAATGC 1 cut(s) 1153
PfeI GAWTC 6 cut(s) 430, 717, 797, 923, 1269, 1790
PflMI CCANNNNNTGG 2 cut(s) 1127, 1190
PfoI TCCNGGA 1 cut(s) 1674
PleI GAGTC 1 cut(s) 280
PmaCI CACGTG 1 cut(s) 1955
PmlI CACGTG 1 cut(s) 1955
PpsI GAGTC 1 cut(s) 280
Ppu21I YACGTR 3 cut(s) 875, 932, 1955
PpuMI RGGWCCY 1 cut(s) 1060
Psp124BI GAGCTC 2 cut(s) 21, 264
Psp5II RGGWCCY 1 cut(s) 1060
Psp6I CCWGG 6 cut(s) 318, 565, 663, 828, 1383, 1674
PspCI CACGTG 1 cut(s) 1955
PspEI GGTNACC 1 cut(s) 608
PspFI CCCAGC 2 cut(s) 1548, 1850
PspGI CCWGG 6 cut(s) 318, 565, 663, 828, 1383, 1674
PspN4I GGNNCC 3 cut(s) 37, 1062, 1554
PspPI GGNCC 4 cut(s) 1060, 1229, 1381, 1672
PspPPI RGGWCCY 1 cut(s) 1060
PstI CTGCAG 2 cut(s) 787, 847
PstNI CAGNNNCTG 1 cut(s) 1850
PsuI RGATCY 1 cut(s) 596
PvuII CAGCTG 2 cut(s) 743, 788
RsaI GTAC 4 cut(s) 442, 645, 896, 1651
RsaNI GTAC 4 cut(s) 441, 644, 895, 1650
RseI CAYNNNNRTG 1 cut(s) 707
SacI GAGCTC 2 cut(s) 21, 264
SalI GTCGAC 1 cut(s) 311
SapI GCTCTTC 1 cut(s) 1273
SaqAI TTAA 4 cut(s) 447, 522, 912, 1692
Sau3AI GATC 5 cut(s) 580, 596, 939, 1027, 1634
Sau96I GGNCC 4 cut(s) 1060, 1229, 1381, 1672
ScaI AGTACT 1 cut(s) 1651
SchI GAGTC 1 cut(s) 281
ScrFI CCNGG 7 cut(s) 320, 567, 665, 830, 1064, 1385, 1676
SduI GDGCHC 4 cut(s) 21, 264, 1555, 1936
SfaNI GCATC 5 cut(s) 744, 959, 1195, 1597, 1837
SfcI CTRYAG 4 cut(s) 28, 783, 843, 1656
SgrDI CGTCGACG 1 cut(s) 311
SinI GGWCC 3 cut(s) 1060, 1229, 1672
SmiMI CAYNNNNRTG 1 cut(s) 707
SmlI CTYRAG 1 cut(s) 730
SmoI CTYRAG 1 cut(s) 730
SnaBI TACGTA 1 cut(s) 875
SsiI CCGC 7 cut(s) 114, 121, 178, 282, 285, 288, 1729
SspI AATATT 1 cut(s) 104
SspMI CTAG 4 cut(s) 576, 750, 1614, 1685
SstI GAGCTC 2 cut(s) 21, 264
StyD4I CCNGG 7 cut(s) 318, 565, 663, 828, 1062, 1383, 1674
StyI CCWWGG 2 cut(s) 473, 1543
TaaI ACNGT 3 cut(s) 445, 652, 1621
TaiI ACGT 4 cut(s) 312, 877, 934, 1957
TaqI TCGA 5 cut(s) 312, 334, 418, 1330, 1785
TaqII GACCGA 2 cut(s) 511, 1974
TatI WGTACW 4 cut(s) 440, 643, 894, 1649
TauI GCSGC 4 cut(s) 285, 288, 291, 1731
TfiI GAWTC 6 cut(s) 430, 717, 797, 923, 1269, 1790
Tru1I TTAA 4 cut(s) 447, 522, 912, 1692
Tru9I TTAA 4 cut(s) 447, 522, 912, 1692
TscAI CASTG 1 cut(s) 1195
TseFI GTSAC 2 cut(s) 608, 1955
TseI GCWGC 7 cut(s) 5, 740, 785, 845, 1610, 1751, 1922
Tsp45I GTSAC 2 cut(s) 608, 1955
TspDTI ATGAA 2 cut(s) 630, 1284
TspGWI ACGGA 1 cut(s) 1829
TspRI CASTG 1 cut(s) 1195
Van91I CCANNNNNTGG 2 cut(s) 1127, 1190
VpaK11BI GGWCC 3 cut(s) 1060, 1229, 1672
XapI RAATTY 6 cut(s) 194, 617, 858, 956, 1220, 1244
XcmI CCANNNNNNNNNTGG 1 cut(s) 410
XmiI GTMKAC 1 cut(s) 312
XspI CTAG 4 cut(s) 576, 750, 1614, 1685
ZraI GACGTC 1 cut(s) 310
ZrmI AGTACT 1 cut(s) 1651
Zsp2I ATGCAT 1 cut(s) 1153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.