Rroxscaffold_6G00393360

KH-domain-like of EngA bacterial GTPase enzymes, C-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0001492
Physical Location & Seq
Reverse (-)
13401981 .. 13410119
8139 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00393360.1

Sequence Viewer

Length: 2001 bp
ATGGCAGCTGCTGCTGAGCTCTTCTTGTCTTACAGAGGAGGCGGAGGCGGAGGCGGAGGAACCACCGCTCTGTCCAAAACCCTATCTCTTGTTTCTTCCCCATTTCCCAAATCAATTCTTCATCTTCTCCGCACCACTCCCTCCTCCCCTTCCCTTTCCCTTTCCCTTTCCCTCCACCACCACCACCAGGCTTCTCGTCTTCCTCTCCGCTTGCTCTCCGCCACTGATCATCTCGACGTTTCCCTAACAGACGACGACGACGAAGAACAAGAACTAGAACCAGAGCCAGAGGGAGATGAGTCCGACGTCGATTTGGACCTCGACGCCCTCGAACAAGACGCCCTTCTCGCCGTCCGAGACTTCTCTTCATCTCTCTCCAACCAGATCACAATCGAAGATGAGAAAACTGATCAAGAGGCACTCGGCCGAAAGCAGAGGAGGCAGAAAACTCCAATCAAAAATAAAACTATCCCTGACCATCTTCTTCCGAGAGTTGCAATTGTTGGAAGACCAAATGTTGGTAAATCAGCATTATTCAATCGACTTGTCTCGGGGAACAAGGCAATTGTAGTTGATGAGCCAGGGGTCACTAGGGATCGCCTGTATGGTAGATCCTTTTGGGGGGACTATGAATTTATGGTGGTCGATACTGGTGGTGTTCTTACAGTTTCAAAGTCACAGGCCAACGTCATGGAAGAACTGGCCATTTCGACGACCATCGGTATGGATGGAATTCCACTTGCCTCCAGAGAGGCAGCGGTTGCAAGGATGCCCTCAATGATTGAGAAACAAGCTACTGCAGCTGTTGAAGAGTCATCTGTCATTATTTTTCTTGTTGATGGCCAGGCTGGTCTAACTGCGGCTGATGAGGAAATTTCAGATTGGCTACGTAGGAACTTCTCAGATAAGTACATCATTCTTGCAGTCAACAAATGTGAATCTCCACGTAAAGGAGTCATGCAAGCATCAGAGTTTTGGTCTTTGGGGTTTTCGCCGCTTCCAATATCTGCTATATCTGGAACTGGAACCGGAGAGCTTCTTGATCTTGTGTGTTCAGGCCTAAAAAAGATCGAGGATCCTGATGATTTTGCTGAACAAGAAGATTATGTTCCTGCAATTGCAATTGTTGGTCGACCAAATGTTGGTAAAAGTAGCATTTTGAACGCGTTGGTTGGAGAGGACAGGACAATTGTTAGCCCAGTCAGTGGAACTACTCGCGATGCTATTGATATTGAATTCACTGGACCGGATGGACAGAAGTTCCGGCTTATTGACACTGCTGGAATCAGAAGAAAAGCCGTCGTTGCTTCATCAGGTAGTGTGACGGAGGCTTTATCGGTTAATCGAGCATTTCGTGCCATTCGCCGTTCTGATGTTGTGGCTCTTGTCATTGAAGCCATGGCTTGTATCACAGAACAGGATTGCAAGATTGCTGAAAGGATAGAAAGAGAAGGGAAAGGTTGCCTGATAGTTGTAAACAAATGGGACACTATACCAAATAAAAACCAGCAGACTGCAACATACTACGAGCAGGATGTTAGGGAAAAGCTTCGTATTCTTGATTGGGCGCCGGTTGTATATTCAACTGCACTAGCTGGTCGTAGCGTCGATAAAATTATCATGGCCGCTAGCACTGTTGAGAAAGAACGGTCAAGAAGGCTTACGACTGCTGTGGTAAATCAAGTGGTACAGGAGGCACTAGCTTTTAAATCACCACCTAGGACACGAGGTGGCAAAAGAGGACGTATTTATTACAGCACTCAGTTCTTGCCCAAAGGCGCTCTTGTCTCTAATTCTAAATTCATTTCGTTAGCTGCCATAAGGCCACCAACATTTGTATTCTTTGTTAATGATGCGAAACTTTTCCCTGAAACTTATCGTCGCTATATGGAGAAGCAACTGCGTTCAGATGCTGGGTTTGCGGGTACTCCAATTCGCCTTTTATGGAGCAACAGGAGGAAGACAGAAAGAGATGAAGGTCAGTTCTCATCTTGTCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KH_dom-like MMR_HSR1

Protein Analysis

666

Amino Acids

72.93

Weight (kDa)

5.84

Isoelectric Point (pI)

48.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MMR_HSR1 PF01926 164 - 311 3.7e-23 50S ribosome-binding GTPase
FeoB_N PF02421 164 - 226 3.5e-08 Ferrous iron transport protein B
MMR_HSR1 PF01926 373 - 494 3.9e-27 50S ribosome-binding GTPase
FeoB_N PF02421 373 - 539 3.3e-09 Ferrous iron transport protein B
KH_dom-like PF14714 553 - 649 5.3e-22 KH-domain-like of EngA bacterial GTPase enzymes, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 309
AccB1I GGYRCC 1 cut(s) 1567
AccB7I CCANNNNNTGG 3 cut(s) 518, 1142, 1205
AccBSI CCGCTC 1 cut(s) 68
AccI GTMKAC 1 cut(s) 1132
AccII CGCG 2 cut(s) 1166, 1218
AclWI GGATC 4 cut(s) 603, 606, 1070, 1083
AcoI YGGCCR 4 cut(s) 424, 702, 841, 1623
AcsI RAATTY 5 cut(s) 632, 732, 873, 1235, 1799
AcyI GRCGYC 4 cut(s) 306, 324, 339, 1568
AdeI CACNNNGTG 1 cut(s) 1730
AfaI GTAC 3 cut(s) 911, 1689, 1927
AfiI CCNNNNNNNGG 6 cut(s) 187, 518, 621, 950, 1142, 1205
AflIII ACRYGT 1 cut(s) 1164
AgsI TTSAA 7 cut(s) 538, 672, 809, 1162, 1235, 1394, 1584
AjnI CCWGG 3 cut(s) 186, 580, 843
AluBI AGCT 9 cut(s) 8, 19, 794, 803, 1036, 1549, 1595, 1703, 1814
AluI AGCT 9 cut(s) 8, 19, 794, 803, 1036, 1549, 1595, 1703, 1814
Alw21I GWGCWC 1 cut(s) 21
Alw26I GTCTC 3 cut(s) 351, 553, 1792
AlwI GGATC 4 cut(s) 603, 606, 1070, 1083
AlwNI CAGNNNCTG 2 cut(s) 11, 1913
Ama87I CYCGRG 1 cut(s) 550
AoxI GGCC 7 cut(s) 424, 681, 702, 841, 1057, 1623, 1823
ApeKI GCWGC 6 cut(s) 5, 8, 11, 755, 800, 1814
ApoI RAATTY 5 cut(s) 632, 732, 873, 1235, 1799
Asp700I GAANNNNTTC 2 cut(s) 1548, 1862
AspA2I CCTAGG 1 cut(s) 1718
AspLEI GCGC 2 cut(s) 1570, 1781
AspS9I GGNCC 2 cut(s) 316, 1244
AsuHPI GGTGA 1 cut(s) 1704
AsuNHI GCTAGC 1 cut(s) 1628
AvaI CYCGRG 1 cut(s) 550
AvaII GGWCC 2 cut(s) 316, 1244
AvrII CCTAGG 1 cut(s) 1718
BalI TGGCCA 2 cut(s) 704, 843
BamHI GGATCC 1 cut(s) 1075
BanI GGYRCC 1 cut(s) 1567
BanII GRGCYC 1 cut(s) 21
BauI CACGAG 1 cut(s) 1725
BbsI GAAGAC 3 cut(s) 191, 514, 1967
Bbv12I GWGCWC 1 cut(s) 21
BbvI GCAGC 4 cut(s) 17, 767, 812, 1801
BccI CCATC 5 cut(s) 486, 722, 725, 833, 1244
BceAI ACGGC 3 cut(s) 335, 1283, 1350
BciT130I CCWGG 3 cut(s) 188, 582, 845
BclI TGATCA 2 cut(s) 226, 409
BcoDI GTCTC 3 cut(s) 351, 553, 1792
BfaI CTAG 6 cut(s) 275, 591, 1592, 1629, 1700, 1719
BfmI CTRYAG 1 cut(s) 798
BfoI RGCGCY 2 cut(s) 1571, 1782
BisI GCNGC 9 cut(s) 6, 9, 12, 756, 801, 861, 995, 1626, 1815
BlnI CCTAGG 1 cut(s) 1718
BlpI GCTNAGC 1 cut(s) 15
BlsI GCNGC 9 cut(s) 7, 10, 13, 757, 802, 862, 996, 1627, 1816
Bme1390I CCNGG 3 cut(s) 188, 582, 845
Bme18I GGWCC 2 cut(s) 316, 1244
BmeT110I CYCGRG 1 cut(s) 550
BmgT120I GGNCC 2 cut(s) 316, 1244
BmiI GGNNCC 4 cut(s) 61, 1027, 1077, 1569
BmrFI CCNGG 3 cut(s) 188, 582, 845
BmrI ACTGGG 1 cut(s) 1193
BmsI GCATC 5 cut(s) 759, 974, 1210, 1843, 1900
BmtI GCTAGC 1 cut(s) 1632
BmuI ACTGGG 1 cut(s) 1193
BpiI GAAGAC 3 cut(s) 191, 514, 1967
BpmI CTGGAG 1 cut(s) 730
Bpu1102I GCTNAGC 1 cut(s) 15
BsaAI YACGTR 2 cut(s) 890, 947
BsaBI GATNNNNATC 1 cut(s) 389
BsaHI GRCGYC 4 cut(s) 306, 324, 339, 1568
BsaJI CCNNGG 3 cut(s) 581, 1398, 1718
BsaWI WCCGGW 2 cut(s) 1028, 1246
Bsc4I CCNNNNNNNGG 6 cut(s) 187, 518, 621, 950, 1142, 1205
Bse118I RCCGGY 1 cut(s) 1570
Bse1I ACTGG 5 cut(s) 655, 705, 1027, 1199, 1246
Bse8I GATNNNNATC 1 cut(s) 389
BseBI CCWGG 3 cut(s) 188, 582, 845
BseDI CCNNGG 3 cut(s) 581, 1398, 1718
BseGI GGATG 4 cut(s) 733, 774, 1255, 1540
BseJI GATNNNNATC 1 cut(s) 389
BseLI CCNNNNNNNGG 6 cut(s) 187, 518, 621, 950, 1142, 1205
BseMII CTCAG 3 cut(s) 6, 915, 1775
BseNI ACTGG 5 cut(s) 655, 705, 1027, 1199, 1246
BseRI GAGGAG 3 cut(s) 51, 133, 451
BseX3I CGGCCG 1 cut(s) 424
BseXI GCAGC 4 cut(s) 17, 767, 812, 1801
BseYI CCCAGC 1 cut(s) 1913
BsgI GTGCAG 1 cut(s) 1572
Bsh1236I CGCG 2 cut(s) 1166, 1218
Bsh1285I CGRYCG 1 cut(s) 427
BshFI GGCC 7 cut(s) 426, 683, 704, 843, 1059, 1625, 1825
BshNI GGYRCC 1 cut(s) 1567
BsiEI CGRYCG 1 cut(s) 427
BsiHKAI GWGCWC 1 cut(s) 21
BsiHKCI CYCGRG 1 cut(s) 550
BsiSI CCGG 4 cut(s) 1029, 1247, 1264, 1571
BslFI GGGAC 2 cut(s) 638, 1499
BslI CCNNNNNNNGG 6 cut(s) 187, 518, 621, 950, 1142, 1205
BsmAI GTCTC 3 cut(s) 351, 553, 1792
BsmFI GGGAC 2 cut(s) 638, 1499
BsnI GGCC 7 cut(s) 426, 683, 704, 843, 1059, 1625, 1825
BsoBI CYCGRG 1 cut(s) 550
Bsp1286I GDGCHC 1 cut(s) 21
Bsp143I GATC 8 cut(s) 226, 384, 409, 595, 611, 1042, 1068, 1075
Bsp1720I GCTNAGC 1 cut(s) 15
Bsp19I CCATGG 1 cut(s) 1398
Bsp68I TCGCGA 1 cut(s) 1218
BspANI GGCC 7 cut(s) 426, 683, 704, 843, 1059, 1625, 1825
BspCNI CTCAG 3 cut(s) 7, 914, 1774
BspFNI CGCG 2 cut(s) 1166, 1218
BspLI GGNNCC 4 cut(s) 61, 1027, 1077, 1569
BspMAI CTGCAG 1 cut(s) 802
BspOI GCTAGC 1 cut(s) 1632
BspPI GGATC 4 cut(s) 603, 606, 1070, 1083
BspQI GCTCTTC 1 cut(s) 26
BspT107I GGYRCC 1 cut(s) 1567
BsrBI CCGCTC 1 cut(s) 68
BsrFI RCCGGY 1 cut(s) 1570
BsrI ACTGG 5 cut(s) 655, 705, 1027, 1199, 1246
BssAI RCCGGY 1 cut(s) 1570
BssECI CCNNGG 3 cut(s) 581, 1398, 1718
BssMI GATC 8 cut(s) 226, 384, 409, 595, 611, 1042, 1068, 1075
BssNI GRCGYC 4 cut(s) 306, 324, 339, 1568
BssSI CACGAG 1 cut(s) 1725
BssT1I CCWWGG 2 cut(s) 1398, 1718
Bst2BI CACGAG 1 cut(s) 1725
Bst2UI CCWGG 3 cut(s) 188, 582, 845
Bst4CI ACNGT 3 cut(s) 667, 1636, 1650
Bst6I CTCTTC 3 cut(s) 26, 370, 804
BstACI GRCGYC 4 cut(s) 306, 324, 339, 1568
BstAPI GCANNNNNTGC 3 cut(s) 11, 761, 1355
BstBAI YACGTR 2 cut(s) 890, 947
BstC8I GCNNGC 3 cut(s) 212, 963, 1630
BstDEI CTNAG 3 cut(s) 15, 901, 1761
BstDSI CCRYGG 1 cut(s) 1398
BstF5I GGATG 4 cut(s) 733, 774, 1255, 1540
BstFNI CGCG 2 cut(s) 1166, 1218
BstH2I RGCGCY 2 cut(s) 1571, 1782
BstHHI GCGC 2 cut(s) 1570, 1781
BstKTI GATC 8 cut(s) 229, 387, 412, 598, 614, 1045, 1071, 1078
BstMAI GTCTC 3 cut(s) 351, 553, 1792
BstMBI GATC 8 cut(s) 226, 384, 409, 595, 611, 1042, 1068, 1075
BstMCI CGRYCG 1 cut(s) 427
BstMWI GCNNNNNNNGC 8 cut(s) 11, 347, 439, 761, 800, 1304, 1355, 1919
BstNI CCWGG 3 cut(s) 188, 582, 845
BstSCI CCNGG 3 cut(s) 186, 580, 843
BstSFI CTRYAG 1 cut(s) 798
BstSNI TACGTA 1 cut(s) 890
BstUI CGCG 2 cut(s) 1166, 1218
BstV1I GCAGC 4 cut(s) 17, 767, 812, 1801
BstV2I GAAGAC 3 cut(s) 191, 514, 1967
BstX2I RGATCY 2 cut(s) 611, 1075
BstXI CCANNNNNNTGG 2 cut(s) 691, 724
BstYI RGATCY 2 cut(s) 611, 1075
BstZI CGGCCG 1 cut(s) 424
BsuRI GGCC 7 cut(s) 426, 683, 704, 843, 1059, 1625, 1825
BtgI CCRYGG 1 cut(s) 1398
BtgZI GCGATG 1 cut(s) 1233
BtsCI GGATG 4 cut(s) 733, 774, 1255, 1540
BtsI GCAGTG 1 cut(s) 1275
BtsIMutI CAGTG 5 cut(s) 222, 1210, 1239, 1275, 1632
BtuMI TCGCGA 1 cut(s) 1218
Cac8I GCNNGC 3 cut(s) 212, 963, 1630
CaiI CAGNNNCTG 2 cut(s) 11, 1913
CfoI GCGC 2 cut(s) 1570, 1781
Cfr10I RCCGGY 1 cut(s) 1570
Cfr13I GGNCC 2 cut(s) 316, 1244
CseI GACGC 3 cut(s) 332, 347, 1594
Csp6I GTAC 3 cut(s) 910, 1688, 1926
CviAII CATG 4 cut(s) 691, 958, 1399, 1621
CviQI GTAC 3 cut(s) 910, 1688, 1926
DdeI CTNAG 3 cut(s) 15, 901, 1761
DinI GGCGCC 1 cut(s) 1569
DpnI GATC 8 cut(s) 228, 386, 411, 597, 613, 1044, 1070, 1077
DpnII GATC 8 cut(s) 226, 384, 409, 595, 611, 1042, 1068, 1075
DraI TTTAAA 1 cut(s) 1708
DraIII CACNNNGTG 1 cut(s) 1730
EaeI YGGCCR 4 cut(s) 424, 702, 841, 1623
EagI CGGCCG 1 cut(s) 424
Eam1104I CTCTTC 3 cut(s) 26, 370, 804
EarI CTCTTC 3 cut(s) 26, 370, 804
EciI GGCGGA 4 cut(s) 57, 63, 69, 208
Ecl136II GAGCTC 1 cut(s) 19
EclXI CGGCCG 1 cut(s) 424
Eco105I TACGTA 1 cut(s) 890
Eco130I CCWWGG 2 cut(s) 1398, 1718
Eco147I AGGCCT 1 cut(s) 1059
Eco24I GRGCYC 1 cut(s) 21
Eco47I GGWCC 2 cut(s) 316, 1244
Eco52I CGGCCG 1 cut(s) 424
Eco53kI GAGCTC 1 cut(s) 19
Eco88I CYCGRG 1 cut(s) 550
EcoICRI GAGCTC 1 cut(s) 19
EcoRI GAATTC 2 cut(s) 732, 1235
EcoRII CCWGG 3 cut(s) 186, 580, 843
EcoT14I CCWWGG 2 cut(s) 1398, 1718
EcoT38I GRGCYC 1 cut(s) 21
EgeI GGCGCC 1 cut(s) 1569
EheI GGCGCC 1 cut(s) 1569
ErhI CCWWGG 2 cut(s) 1398, 1718
FaeI CATG 4 cut(s) 694, 961, 1402, 1624
FalI AAGNNNNNCTT 4 cut(s) 327, 359, 1767, 1799
FaqI GGGAC 2 cut(s) 638, 1499
FatI CATG 4 cut(s) 690, 957, 1398, 1620
FauI CCCGC 1 cut(s) 1915
FbaI TGATCA 2 cut(s) 226, 409
FblI GTMKAC 1 cut(s) 1132
Fnu4HI GCNGC 9 cut(s) 6, 9, 12, 756, 801, 861, 995, 1626, 1815
FokI GGATG 4 cut(s) 740, 781, 1262, 1547
FriOI GRGCYC 1 cut(s) 21
Fsp4HI GCNGC 9 cut(s) 6, 9, 12, 756, 801, 861, 995, 1626, 1815
FspBI CTAG 6 cut(s) 275, 591, 1592, 1629, 1700, 1719
GlaI GCGC 2 cut(s) 1569, 1780
GluI GCNGC 9 cut(s) 6, 9, 12, 756, 801, 861, 995, 1626, 1815
GsaI CCCAGC 1 cut(s) 1917
GsuI CTGGAG 1 cut(s) 730
HaeII RGCGCY 2 cut(s) 1571, 1782
HaeIII GGCC 7 cut(s) 426, 683, 704, 843, 1059, 1625, 1825
HapII CCGG 4 cut(s) 1029, 1247, 1264, 1571
HgaI GACGC 3 cut(s) 332, 347, 1594
HhaI GCGC 2 cut(s) 1570, 1781
Hin1I GRCGYC 4 cut(s) 306, 324, 339, 1568
Hin1II CATG 4 cut(s) 694, 961, 1402, 1624
Hin6I GCGC 2 cut(s) 1568, 1779
HinP1I GCGC 2 cut(s) 1568, 1779
HincII GTYRAC 2 cut(s) 928, 1133
HindII GTYRAC 2 cut(s) 928, 1133
HindIII AAGCTT 1 cut(s) 1547
HinfI GANTC 5 cut(s) 299, 812, 938, 954, 1284
HpaII CCGG 4 cut(s) 1029, 1247, 1264, 1571
HphI GGTGA 1 cut(s) 1704
Hpy166II GTNNAC 3 cut(s) 928, 1133, 1477
Hpy188I TCNGA 9 cut(s) 304, 356, 489, 880, 904, 970, 1289, 1372, 1909
Hpy188III TCNNGA 9 cut(s) 233, 413, 747, 1017, 1040, 1079, 1217, 1559, 1653
Hpy8I GTNNAC 3 cut(s) 928, 1133, 1477
HpyAV CCTTC 5 cut(s) 159, 353, 1445, 1650, 1970
HpyCH4III ACNGT 3 cut(s) 667, 1636, 1650
HpyCH4IV ACGT 6 cut(s) 237, 306, 687, 889, 946, 1744
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 347, 439, 761, 800, 1304, 1355, 1919
HpyF3I CTNAG 3 cut(s) 15, 901, 1761
HpySE526I ACGT 6 cut(s) 237, 306, 687, 889, 946, 1744
Hsp92I GRCGYC 4 cut(s) 306, 324, 339, 1568
Hsp92II CATG 4 cut(s) 694, 961, 1402, 1624
HspAI GCGC 2 cut(s) 1568, 1779
KasI GGCGCC 1 cut(s) 1567
Ksp22I TGATCA 2 cut(s) 226, 409
Kzo9I GATC 8 cut(s) 226, 384, 409, 595, 611, 1042, 1068, 1075
LguI GCTCTTC 1 cut(s) 26
LmnI GCTCC 1 cut(s) 1947
Lsp1109I GCAGC 4 cut(s) 17, 767, 812, 1801
LweI GCATC 5 cut(s) 759, 974, 1210, 1843, 1900
MaeI CTAG 6 cut(s) 275, 591, 1592, 1629, 1700, 1719
MaeII ACGT 6 cut(s) 237, 306, 687, 889, 946, 1744
MaeIII GTNAC 3 cut(s) 586, 675, 1321
MalI GATC 8 cut(s) 228, 386, 411, 597, 613, 1044, 1070, 1077
MbiI CCGCTC 1 cut(s) 68
MboI GATC 8 cut(s) 226, 384, 409, 595, 611, 1042, 1068, 1075
MfeI CAATTG 5 cut(s) 498, 564, 1116, 1122, 1188
MflI RGATCY 2 cut(s) 611, 1075
MhlI GDGCHC 1 cut(s) 21
MlsI TGGCCA 2 cut(s) 704, 843
MluI ACGCGT 1 cut(s) 1164
MluNI TGGCCA 2 cut(s) 704, 843
Mly113I GGCGCC 1 cut(s) 1568
MlyI GAGTC 3 cut(s) 308, 821, 963
MmeI TCCRAC 4 cut(s) 327, 402, 484, 1153
Mox20I TGGCCA 2 cut(s) 704, 843
MroXI GAANNNNTTC 2 cut(s) 1548, 1862
MscI TGGCCA 2 cut(s) 704, 843
MseI TTAA 4 cut(s) 1341, 1707, 1848, 1999
MslI CAYNNNNRTG 1 cut(s) 722
Msp20I TGGCCA 2 cut(s) 704, 843
MspA1I CMGCKG 3 cut(s) 8, 758, 803
MspI CCGG 4 cut(s) 1029, 1247, 1264, 1571
MspR9I CCNGG 3 cut(s) 188, 582, 845
MunI CAATTG 5 cut(s) 498, 564, 1116, 1122, 1188
MvaI CCWGG 3 cut(s) 188, 582, 845
MvnI CGCG 2 cut(s) 1166, 1218
MwoI GCNNNNNNNGC 8 cut(s) 11, 347, 439, 761, 800, 1304, 1355, 1919
NarI GGCGCC 1 cut(s) 1568
NcoI CCATGG 1 cut(s) 1398
NdeII GATC 8 cut(s) 226, 384, 409, 595, 611, 1042, 1068, 1075
NheI GCTAGC 1 cut(s) 1628
NlaIII CATG 4 cut(s) 694, 961, 1402, 1624
NlaIV GGNNCC 4 cut(s) 61, 1027, 1077, 1569
NmeAIII GCCGAG 1 cut(s) 402
NmuCI GTSAC 3 cut(s) 586, 675, 1321
NruI TCGCGA 1 cut(s) 1218
PceI AGGCCT 1 cut(s) 1059
PciSI GCTCTTC 1 cut(s) 26
PcsI WCGNNNNNNNCGW 3 cut(s) 258, 327, 1351
PdmI GAANNNNTTC 2 cut(s) 1548, 1862
PfeI GAWTC 2 cut(s) 938, 1284
PflMI CCANNNNNTGG 3 cut(s) 518, 1142, 1205
PkrI GCNGC 9 cut(s) 7, 10, 13, 757, 802, 862, 996, 1627, 1816
PleI GAGTC 3 cut(s) 307, 820, 962
PluTI GGCGCC 1 cut(s) 1571
PpsI GAGTC 3 cut(s) 307, 820, 962
Ppu21I YACGTR 2 cut(s) 890, 947
Psp124BI GAGCTC 1 cut(s) 21
Psp6I CCWGG 3 cut(s) 186, 580, 843
PspFI CCCAGC 1 cut(s) 1913
PspGI CCWGG 3 cut(s) 186, 580, 843
PspN4I GGNNCC 4 cut(s) 61, 1027, 1077, 1569
PspPI GGNCC 2 cut(s) 316, 1244
PstI CTGCAG 1 cut(s) 802
PstNI CAGNNNCTG 2 cut(s) 11, 1913
PsuI RGATCY 2 cut(s) 611, 1075
PvuII CAGCTG 2 cut(s) 8, 803
RruI TCGCGA 1 cut(s) 1218
RsaI GTAC 3 cut(s) 911, 1689, 1927
RsaNI GTAC 3 cut(s) 910, 1688, 1926
RseI CAYNNNNRTG 1 cut(s) 722
SacI GAGCTC 1 cut(s) 21
SalI GTCGAC 1 cut(s) 1131
SapI GCTCTTC 1 cut(s) 26
SaqAI TTAA 4 cut(s) 1341, 1707, 1848, 1999
SatI GCNGC 9 cut(s) 6, 9, 12, 756, 801, 861, 995, 1626, 1815
Sau3AI GATC 8 cut(s) 226, 384, 409, 595, 611, 1042, 1068, 1075
Sau96I GGNCC 2 cut(s) 316, 1244
SchI GAGTC 3 cut(s) 308, 821, 963
ScrFI CCNGG 3 cut(s) 188, 582, 845
SduI GDGCHC 1 cut(s) 21
SfaNI GCATC 5 cut(s) 759, 974, 1210, 1843, 1900
SfcI CTRYAG 1 cut(s) 798
SfoI GGCGCC 1 cut(s) 1569
SinI GGWCC 2 cut(s) 316, 1244
SmiMI CAYNNNNRTG 1 cut(s) 722
SnaBI TACGTA 1 cut(s) 890
SseBI AGGCCT 1 cut(s) 1059
SspDI GGCGCC 1 cut(s) 1567
SspMI CTAG 6 cut(s) 275, 591, 1592, 1629, 1700, 1719
SstI GAGCTC 1 cut(s) 21
StuI AGGCCT 1 cut(s) 1059
StyD4I CCNGG 3 cut(s) 186, 580, 843
StyI CCWWGG 2 cut(s) 1398, 1718
TaaI ACNGT 3 cut(s) 667, 1636, 1650
TaiI ACGT 6 cut(s) 240, 309, 690, 892, 949, 1747
TatI WGTACW 1 cut(s) 909
TauI GCSGC 3 cut(s) 863, 997, 1628
TfiI GAWTC 2 cut(s) 938, 1284
Tru1I TTAA 4 cut(s) 1341, 1707, 1848, 1999
Tru9I TTAA 4 cut(s) 1341, 1707, 1848, 1999
TscAI CASTG 5 cut(s) 229, 1210, 1246, 1282, 1639
TseFI GTSAC 3 cut(s) 586, 675, 1321
TseI GCWGC 6 cut(s) 5, 8, 11, 755, 800, 1814
Tsp45I GTSAC 3 cut(s) 586, 675, 1321
TspDTI ATGAA 6 cut(s) 110, 357, 645, 1299, 1792, 1989
TspGWI ACGGA 1 cut(s) 1340
TspRI CASTG 5 cut(s) 229, 1210, 1246, 1282, 1639
Van91I CCANNNNNTGG 3 cut(s) 518, 1142, 1205
VpaK11BI GGWCC 2 cut(s) 316, 1244
XapI RAATTY 5 cut(s) 632, 732, 873, 1235, 1799
XmaJI CCTAGG 1 cut(s) 1718
XmiI GTMKAC 1 cut(s) 1132
XmnI GAANNNNTTC 2 cut(s) 1548, 1862
XspI CTAG 6 cut(s) 275, 591, 1592, 1629, 1700, 1719
ZraI GACGTC 1 cut(s) 307
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.