Rw3G026010

KH-domain-like of EngA bacterial GTPase enzymes, C-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0001492
Physical Location & Seq
Forward (+)
35236301 .. 35245132
8832 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G026010.1

Sequence Viewer

Length: 1965 bp
ATGGCAGCAGCTGCTGAGCTCTTCTTGTCTGGCGGAGGAGGAGGAGGAACCACCGCTCTGTCCAAAACCCTATCTCTTGTTTCTTCCCCTTTTCCCAAATCAATTCTTCATCTTCTCCGCACCACTCCCTCCTCCCCTTCCCTTTCCCTCCACCACCACCACGCTTCTCGTCTTCCTCTCCGCTTGCTCTCCGCCACTGACGTTTCCCTAACAGACGACGACGAAGAACAAGAACTAGAACCAGAAGCAGAAGCAGAGGGAGATGAATCCGACGTCGATTTGGACGTCGACGCCCTCGAACAAGACGCCCTTCTCACCGCCCGAGAATTCTCTTCATCTCTCTCCAACCAGCTCACAATCGAAGATGAGAAAACTGATCAAGAGGCACTCGGCCGAAAGCAGAGGAGGCAGAAAACTCCAATCAAAAATAAAACTATCCCTGACCATCTTCTTCCGAGAGTTGCAATTGTTGGAAGACCAAATGTTGGTAAATCAGCATTATTCAATCGACTTGTCTCGGGGAACAAGGCAATTGTAGTTGATGAGCCAGGGGTCACTAGGGATCGCCTGTATGGTAGATCCTTTTGGGGGGACTTTGAATTTATGGTGGTGGATACTGGTGGTGTTCTTACAGTTTCAAAGTCACAGGCCAACGTCATGGAAGAACTGGCCATTTCAACGACCGTTGGTATGGATGGAATTCCACTTGCCTCCAGAGAGGCAGCGGTTGCAAGGATGCCCTCAATGATTGAGAAACAAGCTACTGCAGCTGTTGAAGAGTCATCTGTCATTATTTTTCTTGTTGATGGCCAGGCTGGTCTAACTGCGGCTGACGAGGAAATTTCAGATTGGCTACGTAGGAACTTCTCAGATAAGTACATCATTCTTGCAGTCAACAAATGTGAATCTCCACGTAAGGGAGTCATGCAAGCATCAGAGTTTTGGTCCTTGGGGTTTTCGCCGCTTCCTATATCTGCTATATCTGGAACTGGAACCGGAGAGCTTCTCGATCTTGTGTGTTCAGGCCTAAAAAAGATTGAGGATCCTGATGATTTTGCTGAACAAGAAGATTATGTTCCTGCAATTGCAATTGTTGGTCGACCAAATGTTGGTAAAAGTAGCATTTTGAACGCGTTGGTTGGAGAAGACAGGACAATTGTTAGCCCAGTCAGTGGAACTACTCGCGATGCTATTGATATTGAATTCACTGGACCAGATGGACAGAAGTTCCGGCTTATTGACACTGCCGGAATCAGAAGAAAAGCCGTTGTTGCTTCATCAGGTAGTGTGACGGAGGCTTTATCAGTTAATCGAGCATTTCGTGCCATTCGCCGTTCTGATGTTGTGGCTCTTGTCATTGAGGCCATGGCTTGTATCACAGAACAGGATTGCAAGATTGCTGAAAGGATAGAAAGAGAAGGGAAAGGTTGCCTGATAGTTGTAAACAAATGGGACACTATACCAAATAAAAACCAGCAGACTGCAACATACTACGAGCAGGATGTGAGGGAAAAGCTTCGTATTCTTGATTGGGCGCCGGTTGTATATTCAACTGCACTAGCTGGTCGTAGTGTTGATAAAATTATCATGGCTGCTAGCACTGTTGAGAAAGAACGGTCAAGAAGGCTTACGACTGCTGTGGTAAATCAAGTGGTACAGGAGGCACTAGCTTTTAAATCACCACCTAGGACACGAGGTGGCAAAAGAGGACGTATTTATTACAGCACGCAGGCTGCTATAAGGCCACCAACATTTGTATTCTTTGTTAATGATGCGAAACTTTTCCCTGAAACTTATCGTCGCTATATGGAGAAGCAACTGCGTTCAGATGCTGGATTTGCGGGTACTCCAATTCGCCTTTTATGGAGCAACAGGAGGAAGACAGAAAGAGATGAAGGGAGAGCTACAACAAGGACAAAAGGGAATCTTGTGCCACGTGACAAAAAATTGGAATTAGCTACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KH_dom-like MMR_HSR1

Protein Analysis

654

Amino Acids

71.59

Weight (kDa)

5.95

Isoelectric Point (pI)

46.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MMR_HSR1 PF01926 153 - 300 7.4e-23 50S ribosome-binding GTPase
FeoB_N PF02421 153 - 215 1.6e-07 Ferrous iron transport protein B
MMR_HSR1 PF01926 362 - 483 3.7e-27 50S ribosome-binding GTPase
FeoB_N PF02421 362 - 528 3.2e-09 Ferrous iron transport protein B
KH_dom-like PF14714 542 - 622 5.4e-28 KH-domain-like of EngA bacterial GTPase enzymes, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 276, 288
AccB1I GGYRCC 1 cut(s) 1534
AccB7I CCANNNNNTGG 3 cut(s) 485, 1109, 1172
AccBSI CCGCTC 1 cut(s) 56
AccI GTMKAC 2 cut(s) 288, 1099
AccII CGCG 2 cut(s) 1133, 1185
AclWI GGATC 4 cut(s) 570, 573, 1037, 1050
AcoI YGGCCR 3 cut(s) 391, 669, 808
AcsI RAATTY 5 cut(s) 326, 599, 699, 840, 1202
AcvI CACGTG 1 cut(s) 1937
AcyI GRCGYC 5 cut(s) 273, 285, 291, 306, 1535
AdeI CACNNNGTG 1 cut(s) 1697
AfaI GTAC 3 cut(s) 878, 1656, 1846
AfiI CCNNNNNNNGG 5 cut(s) 485, 588, 917, 1109, 1172
AflIII ACRYGT 1 cut(s) 1131
AgsI TTSAA 8 cut(s) 505, 599, 639, 678, 776, 1129, 1202, 1551
AjnI CCWGG 2 cut(s) 547, 810
Alw21I GWGCWC 1 cut(s) 21
Alw26I GTCTC 1 cut(s) 520
AlwI GGATC 4 cut(s) 570, 573, 1037, 1050
AlwNI CAGNNNCTG 3 cut(s) 11, 14, 1832
Ama87I CYCGRG 2 cut(s) 321, 517
AoxI GGCC 7 cut(s) 391, 648, 669, 808, 1024, 1362, 1742
ApeKI GCWGC 7 cut(s) 5, 8, 11, 722, 767, 1592, 1733
ApoI RAATTY 5 cut(s) 326, 599, 699, 840, 1202
Asp700I GAANNNNTTC 2 cut(s) 1515, 1781
AspA2I CCTAGG 1 cut(s) 1685
AspLEI GCGC 1 cut(s) 1537
AspS9I GGNCC 2 cut(s) 945, 1211
AsuHPI GGTGA 2 cut(s) 307, 1671
AsuNHI GCTAGC 1 cut(s) 1595
AvaI CYCGRG 2 cut(s) 321, 517
AvaII GGWCC 2 cut(s) 945, 1211
AvrII CCTAGG 1 cut(s) 1685
BalI TGGCCA 2 cut(s) 671, 810
BamHI GGATCC 1 cut(s) 1042
BanI GGYRCC 1 cut(s) 1534
BanII GRGCYC 1 cut(s) 21
BauI CACGAG 1 cut(s) 1692
BbrPI CACGTG 1 cut(s) 1937
BbsI GAAGAC 4 cut(s) 164, 481, 1152, 1886
Bbv12I GWGCWC 1 cut(s) 21
BbvI GCAGC 6 cut(s) 17, 20, 734, 779, 1579, 1720
BccI CCATC 4 cut(s) 453, 689, 800, 1211
BceAI ACGGC 2 cut(s) 1250, 1317
BciT130I CCWGG 2 cut(s) 549, 812
BciVI GTATCC 1 cut(s) 607
BclI TGATCA 1 cut(s) 376
BcoDI GTCTC 1 cut(s) 520
BfaI CTAG 6 cut(s) 236, 558, 1559, 1596, 1667, 1686
BfmI CTRYAG 1 cut(s) 765
BfoI RGCGCY 1 cut(s) 1538
BfuI GTATCC 1 cut(s) 607
BisI GCNGC 9 cut(s) 6, 9, 12, 723, 768, 828, 962, 1593, 1734
BlnI CCTAGG 1 cut(s) 1685
BlpI GCTNAGC 1 cut(s) 15
BlsI GCNGC 9 cut(s) 7, 10, 13, 724, 769, 829, 963, 1594, 1735
Bme1390I CCNGG 2 cut(s) 549, 812
Bme18I GGWCC 2 cut(s) 945, 1211
BmeT110I CYCGRG 2 cut(s) 321, 517
BmgT120I GGNCC 2 cut(s) 945, 1211
BmiI GGNNCC 4 cut(s) 49, 994, 1044, 1536
BmrFI CCNGG 2 cut(s) 549, 812
BmrI ACTGGG 1 cut(s) 1160
BmsI GCATC 5 cut(s) 726, 941, 1177, 1762, 1819
BmtI GCTAGC 1 cut(s) 1599
BmuI ACTGGG 1 cut(s) 1160
BpiI GAAGAC 4 cut(s) 164, 481, 1152, 1886
BplI GAGNNNNNCTC 2 cut(s) 990, 1022
BpmI CTGGAG 1 cut(s) 697
Bpu1102I GCTNAGC 1 cut(s) 15
BsaAI YACGTR 3 cut(s) 857, 914, 1937
BsaHI GRCGYC 5 cut(s) 273, 285, 291, 306, 1535
BsaJI CCNNGG 4 cut(s) 548, 948, 1365, 1685
BsaWI WCCGGW 1 cut(s) 995
Bsc4I CCNNNNNNNGG 5 cut(s) 485, 588, 917, 1109, 1172
Bse118I RCCGGY 1 cut(s) 1537
Bse1I ACTGG 5 cut(s) 622, 672, 994, 1166, 1213
BseBI CCWGG 2 cut(s) 549, 812
BseDI CCNNGG 4 cut(s) 548, 948, 1365, 1685
BseGI GGATG 3 cut(s) 700, 741, 1507
BseLI CCNNNNNNNGG 5 cut(s) 485, 588, 917, 1109, 1172
BseMII CTCAG 2 cut(s) 6, 882
BseNI ACTGG 5 cut(s) 622, 672, 994, 1166, 1213
BseRI GAGGAG 5 cut(s) 51, 54, 57, 121, 418
BseX3I CGGCCG 1 cut(s) 391
BseXI GCAGC 6 cut(s) 17, 20, 734, 779, 1579, 1720
BsgI GTGCAG 1 cut(s) 1539
Bsh1236I CGCG 2 cut(s) 1133, 1185
Bsh1285I CGRYCG 2 cut(s) 394, 684
BshFI GGCC 7 cut(s) 393, 650, 671, 810, 1026, 1364, 1744
BshNI GGYRCC 1 cut(s) 1534
BsiEI CGRYCG 2 cut(s) 394, 684
BsiHKAI GWGCWC 1 cut(s) 21
BsiHKCI CYCGRG 2 cut(s) 321, 517
BsiSI CCGG 4 cut(s) 996, 1231, 1248, 1538
BslFI GGGAC 2 cut(s) 605, 1466
BslI CCNNNNNNNGG 5 cut(s) 485, 588, 917, 1109, 1172
BsmAI GTCTC 1 cut(s) 520
BsmFI GGGAC 2 cut(s) 605, 1466
BsnI GGCC 7 cut(s) 393, 650, 671, 810, 1026, 1364, 1744
BsoBI CYCGRG 2 cut(s) 321, 517
Bsp1286I GDGCHC 1 cut(s) 21
Bsp143I GATC 5 cut(s) 376, 562, 578, 1009, 1042
Bsp1720I GCTNAGC 1 cut(s) 15
Bsp19I CCATGG 1 cut(s) 1365
Bsp68I TCGCGA 1 cut(s) 1185
BspANI GGCC 7 cut(s) 393, 650, 671, 810, 1026, 1364, 1744
BspCNI CTCAG 2 cut(s) 7, 881
BspFNI CGCG 2 cut(s) 1133, 1185
BspLI GGNNCC 4 cut(s) 49, 994, 1044, 1536
BspMAI CTGCAG 1 cut(s) 769
BspOI GCTAGC 1 cut(s) 1599
BspPI GGATC 4 cut(s) 570, 573, 1037, 1050
BspQI GCTCTTC 1 cut(s) 26
BspT107I GGYRCC 1 cut(s) 1534
BsrBI CCGCTC 1 cut(s) 56
BsrFI RCCGGY 1 cut(s) 1537
BsrI ACTGG 5 cut(s) 622, 672, 994, 1166, 1213
BssAI RCCGGY 1 cut(s) 1537
BssECI CCNNGG 4 cut(s) 548, 948, 1365, 1685
BssMI GATC 5 cut(s) 376, 562, 578, 1009, 1042
BssNI GRCGYC 5 cut(s) 273, 285, 291, 306, 1535
BssSI CACGAG 1 cut(s) 1692
BssT1I CCWWGG 3 cut(s) 948, 1365, 1685
Bst2BI CACGAG 1 cut(s) 1692
Bst2UI CCWGG 2 cut(s) 549, 812
Bst4CI ACNGT 4 cut(s) 634, 685, 1603, 1617
Bst6I CTCTTC 3 cut(s) 26, 337, 771
BstACI GRCGYC 5 cut(s) 273, 285, 291, 306, 1535
BstAPI GCANNNNNTGC 3 cut(s) 11, 728, 1322
BstBAI YACGTR 3 cut(s) 857, 914, 1937
BstC8I GCNNGC 5 cut(s) 185, 930, 1597, 1727, 1731
BstDEI CTNAG 2 cut(s) 15, 868
BstDSI CCRYGG 1 cut(s) 1365
BstF5I GGATG 3 cut(s) 700, 741, 1507
BstFNI CGCG 2 cut(s) 1133, 1185
BstH2I RGCGCY 1 cut(s) 1538
BstHHI GCGC 1 cut(s) 1537
BstKTI GATC 5 cut(s) 379, 565, 581, 1012, 1045
BstMAI GTCTC 1 cut(s) 520
BstMBI GATC 5 cut(s) 376, 562, 578, 1009, 1042
BstMCI CGRYCG 2 cut(s) 394, 684
BstMWI GCNNNNNNNGC 7 cut(s) 11, 406, 728, 767, 1271, 1322, 1838
BstNI CCWGG 2 cut(s) 549, 812
BstSCI CCNGG 2 cut(s) 547, 810
BstSFI CTRYAG 1 cut(s) 765
BstSNI TACGTA 1 cut(s) 857
BstUI CGCG 2 cut(s) 1133, 1185
BstV1I GCAGC 6 cut(s) 17, 20, 734, 779, 1579, 1720
BstV2I GAAGAC 4 cut(s) 164, 481, 1152, 1886
BstX2I RGATCY 2 cut(s) 578, 1042
BstXI CCANNNNNNTGG 1 cut(s) 658
BstYI RGATCY 2 cut(s) 578, 1042
BstZI CGGCCG 1 cut(s) 391
BsuI GTATCC 1 cut(s) 607
BsuRI GGCC 7 cut(s) 393, 650, 671, 810, 1026, 1364, 1744
BtgI CCRYGG 1 cut(s) 1365
BtgZI GCGATG 1 cut(s) 1200
BtsCI GGATG 3 cut(s) 700, 741, 1507
BtsI GCAGTG 1 cut(s) 1242
BtsIMutI CAGTG 5 cut(s) 195, 1177, 1206, 1242, 1599
BtuMI TCGCGA 1 cut(s) 1185
Cac8I GCNNGC 5 cut(s) 185, 930, 1597, 1727, 1731
CaiI CAGNNNCTG 3 cut(s) 11, 14, 1832
CfoI GCGC 1 cut(s) 1537
Cfr10I RCCGGY 1 cut(s) 1537
Cfr13I GGNCC 2 cut(s) 945, 1211
CseI GACGC 2 cut(s) 299, 314
Csp6I GTAC 3 cut(s) 877, 1655, 1845
CviAII CATG 5 cut(s) 658, 925, 1366, 1588, 1962
CviQI GTAC 3 cut(s) 877, 1655, 1845
DdeI CTNAG 2 cut(s) 15, 868
DinI GGCGCC 1 cut(s) 1536
DpnI GATC 5 cut(s) 378, 564, 580, 1011, 1044
DpnII GATC 5 cut(s) 376, 562, 578, 1009, 1042
DraI TTTAAA 1 cut(s) 1675
DraIII CACNNNGTG 1 cut(s) 1697
EaeI YGGCCR 3 cut(s) 391, 669, 808
EagI CGGCCG 1 cut(s) 391
Eam1104I CTCTTC 3 cut(s) 26, 337, 771
EarI CTCTTC 3 cut(s) 26, 337, 771
EciI GGCGGA 2 cut(s) 48, 181
Ecl136II GAGCTC 1 cut(s) 19
EclXI CGGCCG 1 cut(s) 391
Eco105I TACGTA 1 cut(s) 857
Eco130I CCWWGG 3 cut(s) 948, 1365, 1685
Eco147I AGGCCT 1 cut(s) 1026
Eco24I GRGCYC 1 cut(s) 21
Eco47I GGWCC 2 cut(s) 945, 1211
Eco52I CGGCCG 1 cut(s) 391
Eco53kI GAGCTC 1 cut(s) 19
Eco72I CACGTG 1 cut(s) 1937
Eco88I CYCGRG 2 cut(s) 321, 517
EcoICRI GAGCTC 1 cut(s) 19
EcoRI GAATTC 3 cut(s) 326, 699, 1202
EcoRII CCWGG 2 cut(s) 547, 810
EcoT14I CCWWGG 3 cut(s) 948, 1365, 1685
EcoT38I GRGCYC 1 cut(s) 21
EgeI GGCGCC 1 cut(s) 1536
EheI GGCGCC 1 cut(s) 1536
ErhI CCWWGG 3 cut(s) 948, 1365, 1685
FaeI CATG 5 cut(s) 661, 928, 1369, 1591, 1965
FalI AAGNNNNNCTT 4 cut(s) 294, 326, 1911, 1943
FaqI GGGAC 2 cut(s) 605, 1466
FatI CATG 5 cut(s) 657, 924, 1365, 1587, 1961
FauI CCCGC 1 cut(s) 1834
FbaI TGATCA 1 cut(s) 376
FblI GTMKAC 2 cut(s) 288, 1099
Fnu4HI GCNGC 9 cut(s) 6, 9, 12, 723, 768, 828, 962, 1593, 1734
FokI GGATG 3 cut(s) 707, 748, 1514
FriOI GRGCYC 1 cut(s) 21
Fsp4HI GCNGC 9 cut(s) 6, 9, 12, 723, 768, 828, 962, 1593, 1734
FspBI CTAG 6 cut(s) 236, 558, 1559, 1596, 1667, 1686
GlaI GCGC 1 cut(s) 1536
GluI GCNGC 9 cut(s) 6, 9, 12, 723, 768, 828, 962, 1593, 1734
GsuI CTGGAG 1 cut(s) 697
HaeII RGCGCY 1 cut(s) 1538
HaeIII GGCC 7 cut(s) 393, 650, 671, 810, 1026, 1364, 1744
HapII CCGG 4 cut(s) 996, 1231, 1248, 1538
HgaI GACGC 2 cut(s) 299, 314
HhaI GCGC 1 cut(s) 1537
Hin1I GRCGYC 5 cut(s) 273, 285, 291, 306, 1535
Hin1II CATG 5 cut(s) 661, 928, 1369, 1591, 1965
Hin6I GCGC 1 cut(s) 1535
HinP1I GCGC 1 cut(s) 1535
HincII GTYRAC 3 cut(s) 289, 895, 1100
HindII GTYRAC 3 cut(s) 289, 895, 1100
HindIII AAGCTT 1 cut(s) 1514
HinfI GANTC 6 cut(s) 266, 779, 905, 921, 1251, 1924
HpaII CCGG 4 cut(s) 996, 1231, 1248, 1538
HphI GGTGA 2 cut(s) 307, 1671
Hpy166II GTNNAC 4 cut(s) 289, 895, 1100, 1444
Hpy188I TCNGA 8 cut(s) 271, 456, 847, 871, 937, 1256, 1339, 1828
Hpy188III TCNNGA 8 cut(s) 380, 714, 984, 1007, 1046, 1184, 1526, 1620
Hpy8I GTNNAC 4 cut(s) 289, 895, 1100, 1444
Hpy99I CGWCG 7 cut(s) 221, 224, 275, 278, 290, 293, 1803
HpyAV CCTTC 5 cut(s) 147, 320, 1412, 1617, 1889
HpyCH4III ACNGT 4 cut(s) 634, 685, 1603, 1617
HpyCH4IV ACGT 8 cut(s) 201, 273, 285, 654, 856, 913, 1711, 1936
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 406, 728, 767, 1271, 1322, 1838
HpyF3I CTNAG 2 cut(s) 15, 868
HpySE526I ACGT 8 cut(s) 201, 273, 285, 654, 856, 913, 1711, 1936
Hsp92I GRCGYC 5 cut(s) 273, 285, 291, 306, 1535
Hsp92II CATG 5 cut(s) 661, 928, 1369, 1591, 1965
HspAI GCGC 1 cut(s) 1535
KasI GGCGCC 1 cut(s) 1534
Ksp22I TGATCA 1 cut(s) 376
Kzo9I GATC 5 cut(s) 376, 562, 578, 1009, 1042
LguI GCTCTTC 1 cut(s) 26
LmnI GCTCC 1 cut(s) 1866
Lsp1109I GCAGC 6 cut(s) 17, 20, 734, 779, 1579, 1720
LweI GCATC 5 cut(s) 726, 941, 1177, 1762, 1819
MaeI CTAG 6 cut(s) 236, 558, 1559, 1596, 1667, 1686
MaeII ACGT 8 cut(s) 201, 273, 285, 654, 856, 913, 1711, 1936
MaeIII GTNAC 4 cut(s) 553, 642, 1288, 1937
MalI GATC 5 cut(s) 378, 564, 580, 1011, 1044
MbiI CCGCTC 1 cut(s) 56
MboI GATC 5 cut(s) 376, 562, 578, 1009, 1042
MfeI CAATTG 5 cut(s) 465, 531, 1083, 1089, 1155
MflI RGATCY 2 cut(s) 578, 1042
MhlI GDGCHC 1 cut(s) 21
MlsI TGGCCA 2 cut(s) 671, 810
MluI ACGCGT 1 cut(s) 1131
MluNI TGGCCA 2 cut(s) 671, 810
Mly113I GGCGCC 1 cut(s) 1535
MlyI GAGTC 2 cut(s) 788, 930
MmeI TCCRAC 4 cut(s) 294, 369, 451, 1120
Mox20I TGGCCA 2 cut(s) 671, 810
MroXI GAANNNNTTC 2 cut(s) 1515, 1781
MscI TGGCCA 2 cut(s) 671, 810
MseI TTAA 3 cut(s) 1308, 1674, 1767
Msp20I TGGCCA 2 cut(s) 671, 810
MspA1I CMGCKG 3 cut(s) 11, 725, 770
MspI CCGG 4 cut(s) 996, 1231, 1248, 1538
MspR9I CCNGG 2 cut(s) 549, 812
MunI CAATTG 5 cut(s) 465, 531, 1083, 1089, 1155
MvaI CCWGG 2 cut(s) 549, 812
MvnI CGCG 2 cut(s) 1133, 1185
MwoI GCNNNNNNNGC 7 cut(s) 11, 406, 728, 767, 1271, 1322, 1838
NarI GGCGCC 1 cut(s) 1535
NcoI CCATGG 1 cut(s) 1365
NdeII GATC 5 cut(s) 376, 562, 578, 1009, 1042
NheI GCTAGC 1 cut(s) 1595
NlaIII CATG 5 cut(s) 661, 928, 1369, 1591, 1965
NlaIV GGNNCC 4 cut(s) 49, 994, 1044, 1536
NmeAIII GCCGAG 1 cut(s) 369
NmuCI GTSAC 4 cut(s) 553, 642, 1288, 1937
NruI TCGCGA 1 cut(s) 1185
PceI AGGCCT 1 cut(s) 1026
PciSI GCTCTTC 1 cut(s) 26
PcsI WCGNNNNNNNCGW 3 cut(s) 282, 294, 1318
PdmI GAANNNNTTC 2 cut(s) 1515, 1781
PfeI GAWTC 4 cut(s) 266, 905, 1251, 1924
PflMI CCANNNNNTGG 3 cut(s) 485, 1109, 1172
PkrI GCNGC 9 cut(s) 7, 10, 13, 724, 769, 829, 963, 1594, 1735
PleI GAGTC 2 cut(s) 787, 929
PluTI GGCGCC 1 cut(s) 1538
PmaCI CACGTG 1 cut(s) 1937
PmlI CACGTG 1 cut(s) 1937
PpsI GAGTC 2 cut(s) 787, 929
Ppu21I YACGTR 3 cut(s) 857, 914, 1937
Psp124BI GAGCTC 1 cut(s) 21
Psp6I CCWGG 2 cut(s) 547, 810
PspCI CACGTG 1 cut(s) 1937
PspGI CCWGG 2 cut(s) 547, 810
PspN4I GGNNCC 4 cut(s) 49, 994, 1044, 1536
PspPI GGNCC 2 cut(s) 945, 1211
PstI CTGCAG 1 cut(s) 769
PstNI CAGNNNCTG 3 cut(s) 11, 14, 1832
PsuI RGATCY 2 cut(s) 578, 1042
PvuII CAGCTG 2 cut(s) 11, 770
RruI TCGCGA 1 cut(s) 1185
RsaI GTAC 3 cut(s) 878, 1656, 1846
RsaNI GTAC 3 cut(s) 877, 1655, 1845
SacI GAGCTC 1 cut(s) 21
SalI GTCGAC 2 cut(s) 287, 1098
SapI GCTCTTC 1 cut(s) 26
SaqAI TTAA 3 cut(s) 1308, 1674, 1767
SatI GCNGC 9 cut(s) 6, 9, 12, 723, 768, 828, 962, 1593, 1734
Sau3AI GATC 5 cut(s) 376, 562, 578, 1009, 1042
Sau96I GGNCC 2 cut(s) 945, 1211
SchI GAGTC 2 cut(s) 788, 930
ScrFI CCNGG 2 cut(s) 549, 812
SduI GDGCHC 1 cut(s) 21
SfaNI GCATC 5 cut(s) 726, 941, 1177, 1762, 1819
SfcI CTRYAG 1 cut(s) 765
SfoI GGCGCC 1 cut(s) 1536
SgrDI CGTCGACG 1 cut(s) 287
SinI GGWCC 2 cut(s) 945, 1211
SnaBI TACGTA 1 cut(s) 857
SseBI AGGCCT 1 cut(s) 1026
SspDI GGCGCC 1 cut(s) 1534
SspMI CTAG 6 cut(s) 236, 558, 1559, 1596, 1667, 1686
SstI GAGCTC 1 cut(s) 21
StuI AGGCCT 1 cut(s) 1026
StyD4I CCNGG 2 cut(s) 547, 810
StyI CCWWGG 3 cut(s) 948, 1365, 1685
TaaI ACNGT 4 cut(s) 634, 685, 1603, 1617
TaiI ACGT 8 cut(s) 204, 276, 288, 657, 859, 916, 1714, 1939
TaqI TCGA 8 cut(s) 276, 288, 297, 360, 508, 1008, 1099, 1312
TatI WGTACW 1 cut(s) 876
TauI GCSGC 2 cut(s) 830, 964
TfiI GAWTC 4 cut(s) 266, 905, 1251, 1924
Tru1I TTAA 3 cut(s) 1308, 1674, 1767
Tru9I TTAA 3 cut(s) 1308, 1674, 1767
TscAI CASTG 5 cut(s) 202, 1177, 1213, 1249, 1606
TseFI GTSAC 4 cut(s) 553, 642, 1288, 1937
TseI GCWGC 7 cut(s) 5, 8, 11, 722, 767, 1592, 1733
Tsp45I GTSAC 4 cut(s) 553, 642, 1288, 1937
TspDTI ATGAA 5 cut(s) 98, 279, 324, 1266, 1908
TspGWI ACGGA 1 cut(s) 1307
TspRI CASTG 5 cut(s) 202, 1177, 1213, 1249, 1606
Van91I CCANNNNNTGG 3 cut(s) 485, 1109, 1172
VpaK11BI GGWCC 2 cut(s) 945, 1211
XapI RAATTY 5 cut(s) 326, 599, 699, 840, 1202
XmaJI CCTAGG 1 cut(s) 1685
XmiI GTMKAC 2 cut(s) 288, 1099
XmnI GAANNNNTTC 2 cut(s) 1515, 1781
XspI CTAG 6 cut(s) 236, 558, 1559, 1596, 1667, 1686
ZraI GACGTC 2 cut(s) 274, 286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.