Rorug04G0071000

KH-domain-like of EngA bacterial GTPase enzymes, C-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0001492
Physical Location & Seq
Forward (+)
10927491 .. 10929710
2220 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0071000.1

Sequence Viewer

Length: 2220 bp
ATGACGACCAGAGCAATTGGTGAAAAGGGCAGCCAAGTCTTTCTCCAAAATCTCAAGATTACCCAGTTGGGGAAGTCGGGCCGAATCGACGAAGCAGTCAAACTTTTCTCACAAATGACTCAAAGGAACACCGTCACATACAACTCCATGATCTCCGCCTACGCCAAGAACGGTAGACTCGGCCATGCACGCCACCTGTTCGATAATATGTCTCACAGAAACTTGGTTTCTTGGAACACTATGATTGCAGGGTACCTGCACAGTAATATGGTGGAGGATGCTTATGGGCTTTTTGTTACCATGCCACAAAGAGACCTTTTCTCTTGGACTTTGATGATTACTTGCTACACACGTAACGGCGAGCTTGACAGAGCCAGACAGCTGTTCAGTTTGCTTCCGGATAAGCGGGACCCGGCTTGTTGGAATGCCATGATTGCAGGGTATGGGAAGAAGGGGAGGTTTGAGGAAGCGAAGAGAATGTTTGATGAAATGCCGGTGAAGAATTTAGTTTCTTGGAATTCGATGCTAGCGGGGTATACCAAGAATGGGGAGATGTGTTTGGGGGTGGAGTTTTTTGAGGAGATGCCGGAGAGGAATGTGGTTTCGTGGAATTTGATGCTGGATGGATTTGTTCAGGTTGGTGAGTTGGATAATGCTTGGCAGTTTTTTGAGAAGATTCCAGAGGCAAATGTTGTTTCGTGGGTGACAATGTTGTGTGGGTTTGCGCAAAATGGGAAGATTGTGCAGGCGGAGGATCTGTTTGAGAAGATGCCGAGTAGAAATGTGGTTGCTTGGAATGCAATGCTTGCAGCCTATGTACAAGACCGCCGAATTGACAAGGCTGCTGAAATATTCAGGGATATGCCGGAGATGGATTCAGTGTCATGGACTACAATGATCAATGGGTATGTTCGCGTTGGCAAGCTTGACGAAGCAAGGCAGTTGCTCAACCGGATGCCGTACAGGAACATTGCAGCACAAACAGCAATGATATCTGGATATGCTCATAATGGAAGGATGGATGAAGCAAGTCAGATCTTCAATCAGATTGCCATGCCTGATGTTGTTTGTTGGAACACCATGATTGCAGGCTATGCTCAGTGTGGCAGAATGGTTGAAGCTCTATCTCTTTTCAGAAACATGACCAACAAGGATGTAGTTTCTTGGAATACTATGATTACTGGTTATGCTCAAGTAGGGGAGATGGACAAAGCACTTCAAATCTTTGAGGAGATGGGGGAGAAGAACATAGTTTCTTGGAATTCTCTAATTACAGGTTATGTGCAGAACGGGTTATATCTGGATGCACTGAAGAGTATTGTGATTATGGGACAGGAAGGAAAGAGGCTTGATCAGTCAACTTTTTCATGTGGACTAAGTGCATGTGCCAATCTTGCAGCTTTTCGACTGGGAAGGCAACTTCACCAAATGGTTGTGAAGACCGGCTATCTAAGTGATTTGTTTGTCAGCAATGCCCTGATTACCATGTATGCTAAATGTGGAATGGTTGCAAATGCTAATCTTGTGTTTGAAGATATCAATCATGGCGATATTGTTTCTTGGAATTCTTTGATATCTGGGTATGCTCTAAATGGATATGGTAAAGAGGCAGTAGCACTCTTTCAAGAGATGCTAATAGAAGGGGTGAATCCAGATCAAGTGACCTTTGTTGGGGTGTTGTCTGCATGCAGTCACAGTGGGCTGGTTGATCAGGGATTAAAACTGTTTAAGAGCATGACTGAAGTTCACCTTATTGAACCTGTAGCCGAACACTATGCTTGCATGGTTGACTTGCTTGGCCGTGCAGGAAGGTTAGAGGAAGCCTTTGAAATGGTAAGGGATATGAAGATCAAGGCAACTGCCAGAATATGGGGTGCATTGCTTGGAGCTTCCAGGATACACCGAAATTTACAATTTGGCAAGTATGCTACCGAGAAGCTTTTAGAACTTGAACCTGATAAAGCTTCAAATTATGTACTCTTATCGAACATGCATGCTGAGGCAGGCAGATGGGATGAGGTTGAGAGAGTCAGGGTGTTAATGAAGGAAAGTGATACAGACAAGCAACCCGGCTGCAGTTGGATTGAAGTCAGGAATCAGGTGCATGCTTTTCTCTTTGATGATCCAGTGCAACCCAGAACATCAGAGATATGTAGTGTATTGAAGTCTCTAACCACAGAGATGACAAACACAAGTTCCTTCATGGCATCTTATGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KH_dom-like MMR_HSR1

Protein Analysis

739

Amino Acids

83.36

Weight (kDa)

5.83

Isoelectric Point (pI)

33.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_1 PF12854 42 - 71 2.6e-07 PPR repeat
PPR_2 PF13041 42 - 74 7.2e-08 PPR repeat family
PPR PF01535 45 - 74 1.3e-07 PPR repeat
PPR_2 PF13041 74 - 104 6.3e-06 PPR repeat family
PPR_1 PF12854 137 - 164 8.5e-06 PPR repeat
PPR_2 PF13041 139 - 168 7.2e-07 PPR repeat family
PPR PF01535 140 - 168 8.9e-09 PPR repeat
PPR PF01535 232 - 262 8.6e-06 PPR repeat
PPR_2 PF13041 260 - 290 7.4e-06 PPR repeat family
PPR_1 PF12854 291 - 319 9.3e-07 PPR repeat
PPR_2 PF13041 292 - 324 2e-07 PPR repeat family
PPR PF01535 294 - 323 4.9e-08 PPR repeat
PPR_1 PF12854 352 - 382 1e-07 PPR repeat
PPR_2 PF13041 353 - 385 1.6e-08 PPR repeat family
PPR PF01535 356 - 385 5.5e-08 PPR repeat
PPR_2 PF13041 384 - 417 1.4e-09 PPR repeat family
PPR_1 PF12854 385 - 412 2.5e-07 PPR repeat
PPR PF01535 387 - 417 1.8e-09 PPR repeat
PPR_2 PF13041 415 - 464 2.3e-06 PPR repeat family
PPR_2 PF13041 517 - 564 1.8e-11 PPR repeat family
PPR PF01535 519 - 549 3.8e-08 PPR repeat
E_motif PF20431 634 - 696 6.9e-24 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 95
Acc16I TGCGCA 1 cut(s) 726
Acc36I ACCTGC 1 cut(s) 264
Acc65I GGTACC 1 cut(s) 252
AccB1I GGYRCC 1 cut(s) 252
AccB7I CCANNNNNTGG 1 cut(s) 1869
AccI GTMKAC 2 cut(s) 175, 536
AccII CGCG 1 cut(s) 915
AccIII TCCGGA 1 cut(s) 397
AciI CCGC 5 cut(s) 156, 406, 530, 749, 826
AclWI GGATC 2 cut(s) 762, 2117
AcoI YGGCCR 2 cut(s) 181, 1798
AcsI RAATTY 6 cut(s) 502, 517, 610, 1261, 1564, 1906
AcuI CTGAAG 2 cut(s) 1331, 1761
AfaI GTAC 4 cut(s) 254, 819, 962, 1977
AfiI CCNNNNNNNGG 4 cut(s) 69, 546, 1671, 1869
AflIII ACRYGT 1 cut(s) 350
AjnI CCWGG 1 cut(s) 1892
AluBI AGCT 8 cut(s) 364, 382, 925, 1121, 1400, 1889, 1939, 1964
AluI AGCT 8 cut(s) 364, 382, 925, 1121, 1400, 1889, 1939, 1964
Alw26I GTCTC 3 cut(s) 216, 306, 2172
AlwI GGATC 2 cut(s) 762, 2117
Aor13HI TCCGGA 1 cut(s) 397
AoxI GGCC 3 cut(s) 79, 181, 1798
ApeKI GCWGC 6 cut(s) 30, 809, 842, 974, 1397, 2073
ApoI RAATTY 6 cut(s) 502, 517, 610, 1261, 1564, 1906
Asp718I GGTACC 1 cut(s) 252
AspLEI GCGC 1 cut(s) 727
AspS9I GGNCC 2 cut(s) 79, 409
AsuC2I CCSGG 2 cut(s) 413, 2070
AsuHPI GGTGA 7 cut(s) 32, 508, 653, 715, 1415, 1657, 1739
AsuNHI GCTAGC 1 cut(s) 526
AvaII GGWCC 1 cut(s) 409
BanI GGYRCC 1 cut(s) 252
BbsI GAAGAC 1 cut(s) 1445
BbvCI CCTCAGC 1 cut(s) 1998
BbvI GCAGC 6 cut(s) 42, 821, 829, 986, 1409, 2060
BccI CCATC 6 cut(s) 617, 865, 1012, 1198, 1228, 2004
BceAI ACGGC 3 cut(s) 373, 943, 1785
BcgI CGANNNNNNTGC 2 cut(s) 1757, 1791
BciT130I CCWGG 1 cut(s) 1894
BciVI GTATCC 1 cut(s) 1890
BclI TGATCA 3 cut(s) 897, 1351, 1708
BcnI CCSGG 2 cut(s) 413, 2070
BcoDI GTCTC 3 cut(s) 216, 306, 2172
BfaI CTAG 1 cut(s) 527
BfmI CTRYAG 2 cut(s) 1761, 2074
BfuAI ACCTGC 1 cut(s) 264
BfuI GTATCC 1 cut(s) 1890
BglII AGATCT 1 cut(s) 1035
BisI GCNGC 6 cut(s) 31, 810, 843, 975, 1398, 2074
BlsI GCNGC 6 cut(s) 32, 811, 844, 976, 1399, 2075
Bme1390I CCNGG 3 cut(s) 413, 1894, 2070
Bme18I GGWCC 1 cut(s) 409
BmgT120I GGNCC 2 cut(s) 79, 409
BmiI GGNNCC 3 cut(s) 254, 410, 411
BmrFI CCNGG 3 cut(s) 413, 1894, 2070
BmrI ACTGGG 2 cut(s) 58, 1418
BmsI GCATC 9 cut(s) 268, 513, 573, 606, 759, 945, 1294, 1620, 2216
BmtI GCTAGC 1 cut(s) 530
BmuI ACTGGG 2 cut(s) 58, 1418
BpiI GAAGAC 1 cut(s) 1445
Bpu10I CCTNAGC 1 cut(s) 1998
BpuEI CTTGAG 2 cut(s) 38, 1176
BpuMI CCSGG 2 cut(s) 413, 2070
BsaAI YACGTR 1 cut(s) 353
BsaBI GATNNNNATC 1 cut(s) 1539
BsaI GGTCTC 1 cut(s) 306
BsaWI WCCGGW 2 cut(s) 397, 951
BsaXI ACNNNNNCTCC 2 cut(s) 743, 773
Bsc4I CCNNNNNNNGG 4 cut(s) 69, 546, 1671, 1869
Bse118I RCCGGY 2 cut(s) 493, 1442
Bse1I ACTGG 4 cut(s) 64, 1186, 1413, 2126
Bse3DI GCAATG 5 cut(s) 807, 969, 993, 1477, 1877
Bse8I GATNNNNATC 1 cut(s) 1539
BseAI TCCGGA 1 cut(s) 397
BseBI CCWGG 1 cut(s) 1894
BseGI GGATG 8 cut(s) 283, 628, 960, 1023, 1027, 1159, 1309, 2020
BseJI GATNNNNATC 1 cut(s) 1539
BseLI CCNNNNNNNGG 4 cut(s) 69, 546, 1671, 1869
BseMI GCAATG 5 cut(s) 807, 969, 993, 1477, 1877
BseMII CTCAG 2 cut(s) 1112, 1989
BseNI ACTGG 4 cut(s) 64, 1186, 1413, 2126
BseRI GAGGAG 2 cut(s) 593, 1244
BseXI GCAGC 6 cut(s) 42, 821, 829, 986, 1409, 2060
BsgI GTGCAG 4 cut(s) 242, 764, 1304, 1824
Bsh1236I CGCG 1 cut(s) 915
BshFI GGCC 3 cut(s) 81, 183, 1800
BshNI GGYRCC 1 cut(s) 252
BsiSI CCGG 8 cut(s) 398, 413, 494, 587, 866, 952, 1443, 2070
BslFI GGGAC 2 cut(s) 422, 1344
BslI CCNNNNNNNGG 4 cut(s) 69, 546, 1671, 1869
BsmAI GTCTC 3 cut(s) 216, 306, 2172
BsmFI GGGAC 2 cut(s) 422, 1344
BsmI GAATGC 2 cut(s) 430, 802
BsnI GGCC 3 cut(s) 81, 183, 1800
Bso31I GGTCTC 1 cut(s) 306
Bsp13I TCCGGA 1 cut(s) 397
Bsp1407I TGTACA 1 cut(s) 817
Bsp143I GATC 9 cut(s) 150, 754, 897, 1035, 1351, 1654, 1708, 1848, 2122
BspACI CCGC 5 cut(s) 156, 406, 530, 749, 826
BspANI GGCC 3 cut(s) 81, 183, 1800
BspCNI CTCAG 2 cut(s) 1111, 1990
BspEI TCCGGA 1 cut(s) 397
BspFNI CGCG 1 cut(s) 915
BspLI GGNNCC 3 cut(s) 254, 410, 411
BspMAI CTGCAG 1 cut(s) 2078
BspMI ACCTGC 1 cut(s) 264
BspOI GCTAGC 1 cut(s) 530
BspPI GGATC 2 cut(s) 762, 2117
BspT107I GGYRCC 1 cut(s) 252
BspTNI GGTCTC 1 cut(s) 306
BsrDI GCAATG 5 cut(s) 807, 969, 993, 1477, 1877
BsrFI RCCGGY 2 cut(s) 493, 1442
BsrGI TGTACA 1 cut(s) 817
BsrI ACTGG 4 cut(s) 64, 1186, 1413, 2126
BssAI RCCGGY 2 cut(s) 493, 1442
BssMI GATC 9 cut(s) 150, 754, 897, 1035, 1351, 1654, 1708, 1848, 2122
BssNAI GTATAC 1 cut(s) 537
Bst1107I GTATAC 1 cut(s) 537
Bst2UI CCWGG 1 cut(s) 1894
Bst4CI ACNGT 5 cut(s) 133, 173, 263, 1697, 1725
Bst6I CTCTTC 2 cut(s) 467, 1307
BstAPI GCANNNNNTGC 2 cut(s) 806, 1094
BstAUI TGTACA 1 cut(s) 817
BstBAI YACGTR 1 cut(s) 353
BstDEI CTNAG 4 cut(s) 1098, 1376, 1451, 1998
BstF5I GGATG 8 cut(s) 283, 628, 960, 1023, 1027, 1159, 1309, 2020
BstFNI CGCG 1 cut(s) 915
BstHHI GCGC 1 cut(s) 727
BstKTI GATC 9 cut(s) 153, 757, 900, 1038, 1354, 1657, 1711, 1851, 2125
BstMAI GTCTC 3 cut(s) 216, 306, 2172
BstMBI GATC 9 cut(s) 150, 754, 897, 1035, 1351, 1654, 1708, 1848, 2122
BstMWI GCNNNNNNNGC 7 cut(s) 189, 434, 797, 806, 983, 1094, 1394
BstNI CCWGG 1 cut(s) 1894
BstNSI RCATGY 5 cut(s) 1386, 1689, 1993, 1997, 2108
BstSCI CCNGG 3 cut(s) 411, 1892, 2068
BstSFI CTRYAG 2 cut(s) 1761, 2074
BstUI CGCG 1 cut(s) 915
BstV1I GCAGC 6 cut(s) 42, 821, 829, 986, 1409, 2060
BstV2I GAAGAC 1 cut(s) 1445
BstX2I RGATCY 2 cut(s) 754, 1035
BstYI RGATCY 2 cut(s) 754, 1035
BstZ17I GTATAC 1 cut(s) 537
BsuI GTATCC 1 cut(s) 1890
BsuRI GGCC 3 cut(s) 81, 183, 1800
BtsCI GGATG 8 cut(s) 283, 628, 960, 1023, 1027, 1159, 1309, 2020
BtsIMutI CAGTG 5 cut(s) 885, 1106, 1307, 1702, 2133
BveI ACCTGC 1 cut(s) 264
CfoI GCGC 1 cut(s) 727
Cfr10I RCCGGY 2 cut(s) 493, 1442
Cfr13I GGNCC 2 cut(s) 79, 409
Csp6I GTAC 4 cut(s) 253, 818, 961, 1976
CviQI GTAC 4 cut(s) 253, 818, 961, 1976
DdeI CTNAG 4 cut(s) 1098, 1376, 1451, 1998
DpnI GATC 9 cut(s) 152, 756, 899, 1037, 1353, 1656, 1710, 1850, 2124
DpnII GATC 9 cut(s) 150, 754, 897, 1035, 1351, 1654, 1708, 1848, 2122
DrdI GACNNNNNNGTC 1 cut(s) 95
DseDI GACNNNNNNGTC 1 cut(s) 95
EaeI YGGCCR 2 cut(s) 181, 1798
Eam1104I CTCTTC 2 cut(s) 467, 1307
EarI CTCTTC 2 cut(s) 467, 1307
EciI GGCGGA 2 cut(s) 145, 764
Eco31I GGTCTC 1 cut(s) 306
Eco32I GATATC 3 cut(s) 993, 1537, 1575
Eco47I GGWCC 1 cut(s) 409
Eco57I CTGAAG 2 cut(s) 1331, 1761
EcoO109I RGGNCCY 1 cut(s) 409
EcoRI GAATTC 3 cut(s) 517, 1261, 1564
EcoRII CCWGG 1 cut(s) 1892
EcoRV GATATC 3 cut(s) 993, 1537, 1575
EcoT22I ATGCAT 1 cut(s) 1995
FalI AAGNNNNNCTT 2 cut(s) 1734, 1766
FaqI GGGAC 2 cut(s) 422, 1344
FauI CCCGC 2 cut(s) 399, 523
FbaI TGATCA 3 cut(s) 897, 1351, 1708
FblI GTMKAC 2 cut(s) 175, 536
Fnu4HI GCNGC 6 cut(s) 31, 810, 843, 975, 1398, 2074
FokI GGATG 8 cut(s) 290, 635, 967, 1030, 1034, 1166, 1316, 2027
Fsp4HI GCNGC 6 cut(s) 31, 810, 843, 975, 1398, 2074
FspBI CTAG 1 cut(s) 527
FspI TGCGCA 1 cut(s) 726
GlaI GCGC 1 cut(s) 726
GluI GCNGC 6 cut(s) 31, 810, 843, 975, 1398, 2074
HaeIII GGCC 3 cut(s) 81, 183, 1800
HapII CCGG 8 cut(s) 398, 413, 494, 587, 866, 952, 1443, 2070
HhaI GCGC 1 cut(s) 727
Hin6I GCGC 1 cut(s) 725
HinP1I GCGC 1 cut(s) 725
HincII GTYRAC 2 cut(s) 1359, 1789
HindII GTYRAC 2 cut(s) 1359, 1789
HindIII AAGCTT 3 cut(s) 923, 1937, 1962
HinfI GANTC 8 cut(s) 84, 118, 177, 676, 875, 1648, 2028, 2095
HpaII CCGG 8 cut(s) 398, 413, 494, 587, 866, 952, 1443, 2070
HphI GGTGA 7 cut(s) 32, 508, 653, 715, 1415, 1657, 1739
Hpy166II GTNNAC 6 cut(s) 176, 537, 1359, 1373, 1747, 1789
Hpy188I TCNGA 4 cut(s) 1035, 1047, 1136, 2146
Hpy188III TCNNGA 8 cut(s) 55, 398, 680, 996, 1301, 1625, 1652, 2092
Hpy8I GTNNAC 6 cut(s) 176, 537, 1359, 1373, 1747, 1789
Hpy99I CGWCG 1 cut(s) 92
HpyAV CCTTC 8 cut(s) 445, 1008, 1331, 1407, 1634, 1803, 2038, 2209
HpyCH4III ACNGT 5 cut(s) 133, 173, 263, 1697, 1725
HpyCH4IV ACGT 1 cut(s) 352
HpyF10VI GCNNNNNNNGC 7 cut(s) 189, 434, 797, 806, 983, 1094, 1394
HpyF3I CTNAG 4 cut(s) 1098, 1376, 1451, 1998
HpySE526I ACGT 1 cut(s) 352
HspAI GCGC 1 cut(s) 725
KflI GGGWCCC 1 cut(s) 409
Kpn2I TCCGGA 1 cut(s) 397
KpnI GGTACC 1 cut(s) 256
Ksp22I TGATCA 3 cut(s) 897, 1351, 1708
Kzo9I GATC 9 cut(s) 150, 754, 897, 1035, 1351, 1654, 1708, 1848, 2122
LmnI GCTCC 1 cut(s) 1886
Lsp1109I GCAGC 6 cut(s) 42, 821, 829, 986, 1409, 2060
LweI GCATC 9 cut(s) 268, 513, 573, 606, 759, 945, 1294, 1620, 2216
MaeI CTAG 1 cut(s) 527
MaeII ACGT 1 cut(s) 352
MaeIII GTNAC 6 cut(s) 133, 295, 353, 703, 1660, 1691
MalI GATC 9 cut(s) 152, 756, 899, 1037, 1353, 1656, 1710, 1850, 2124
MboI GATC 9 cut(s) 150, 754, 897, 1035, 1351, 1654, 1708, 1848, 2122
MfeI CAATTG 1 cut(s) 15
MflI RGATCY 2 cut(s) 754, 1035
MlyI GAGTC 3 cut(s) 112, 171, 2037
MmeI TCCRAC 4 cut(s) 401, 627, 1052, 2060
Mph1103I ATGCAT 1 cut(s) 1995
MroI TCCGGA 1 cut(s) 397
MseI TTAA 3 cut(s) 1718, 1728, 2039
MslI CAYNNNNRTG 1 cut(s) 2180
MspA1I CMGCKG 1 cut(s) 382
MspI CCGG 8 cut(s) 398, 413, 494, 587, 866, 952, 1443, 2070
MspR9I CCNGG 3 cut(s) 413, 1894, 2070
MunI CAATTG 1 cut(s) 15
Mva1269I GAATGC 2 cut(s) 430, 802
MvaI CCWGG 1 cut(s) 1894
MvnI CGCG 1 cut(s) 915
MwoI GCNNNNNNNGC 7 cut(s) 189, 434, 797, 806, 983, 1094, 1394
NciI CCSGG 2 cut(s) 413, 2070
NdeII GATC 9 cut(s) 150, 754, 897, 1035, 1351, 1654, 1708, 1848, 2122
NheI GCTAGC 1 cut(s) 526
NlaIV GGNNCC 3 cut(s) 254, 410, 411
NmeAIII GCCGAG 2 cut(s) 159, 798
NmuCI GTSAC 4 cut(s) 133, 703, 1660, 1691
NsbI TGCGCA 1 cut(s) 726
NsiI ATGCAT 1 cut(s) 1995
NspI RCATGY 5 cut(s) 1386, 1689, 1993, 1997, 2108
PaeI GCATGC 3 cut(s) 1689, 1997, 2108
PctI GAATGC 2 cut(s) 430, 802
PfeI GAWTC 5 cut(s) 84, 676, 875, 1648, 2095
PflMI CCANNNNNTGG 1 cut(s) 1869
PfoI TCCNGGA 1 cut(s) 1892
PkrI GCNGC 6 cut(s) 32, 811, 844, 976, 1399, 2075
PleI GAGTC 3 cut(s) 112, 171, 2036
PpsI GAGTC 3 cut(s) 112, 171, 2036
Ppu21I YACGTR 1 cut(s) 353
PpuMI RGGWCCY 1 cut(s) 409
Psp5II RGGWCCY 1 cut(s) 409
Psp6I CCWGG 1 cut(s) 1892
PspGI CCWGG 1 cut(s) 1892
PspN4I GGNNCC 3 cut(s) 254, 410, 411
PspPI GGNCC 2 cut(s) 79, 409
PspPPI RGGWCCY 1 cut(s) 409
PstI CTGCAG 1 cut(s) 2078
PsuI RGATCY 2 cut(s) 754, 1035
PvuII CAGCTG 1 cut(s) 382
RsaI GTAC 4 cut(s) 254, 819, 962, 1977
RsaNI GTAC 4 cut(s) 253, 818, 961, 1976
RseI CAYNNNNRTG 1 cut(s) 2180
SaqAI TTAA 3 cut(s) 1718, 1728, 2039
SatI GCNGC 6 cut(s) 31, 810, 843, 975, 1398, 2074
Sau3AI GATC 9 cut(s) 150, 754, 897, 1035, 1351, 1654, 1708, 1848, 2122
Sau96I GGNCC 2 cut(s) 79, 409
SchI GAGTC 3 cut(s) 112, 171, 2037
ScrFI CCNGG 3 cut(s) 413, 1894, 2070
SfaNI GCATC 9 cut(s) 268, 513, 573, 606, 759, 945, 1294, 1620, 2216
SfcI CTRYAG 2 cut(s) 1761, 2074
SinI GGWCC 1 cut(s) 409
SmiMI CAYNNNNRTG 1 cut(s) 2180
SmlI CTYRAG 2 cut(s) 53, 1191
SmoI CTYRAG 2 cut(s) 53, 1191
SphI GCATGC 3 cut(s) 1689, 1997, 2108
SsiI CCGC 5 cut(s) 156, 406, 530, 749, 826
SspI AATATT 1 cut(s) 852
SspMI CTAG 1 cut(s) 527
StyD4I CCNGG 3 cut(s) 411, 1892, 2068
TaaI ACNGT 5 cut(s) 133, 173, 263, 1697, 1725
TaiI ACGT 1 cut(s) 355
TaqI TCGA 5 cut(s) 87, 201, 521, 1405, 1985
TatI WGTACW 2 cut(s) 817, 1975
TfiI GAWTC 5 cut(s) 84, 676, 875, 1648, 2095
Tru1I TTAA 3 cut(s) 1718, 1728, 2039
Tru9I TTAA 3 cut(s) 1718, 1728, 2039
TscAI CASTG 5 cut(s) 885, 1106, 1314, 1702, 2133
TseFI GTSAC 4 cut(s) 133, 703, 1660, 1691
TseI GCWGC 6 cut(s) 30, 809, 842, 974, 1397, 2073
Tsp45I GTSAC 4 cut(s) 133, 703, 1660, 1691
TspDTI ATGAA 6 cut(s) 501, 1038, 1356, 1859, 2057, 2191
TspRI CASTG 5 cut(s) 885, 1106, 1314, 1702, 2133
Van91I CCANNNNNTGG 1 cut(s) 1869
VpaK11BI GGWCC 1 cut(s) 409
XapI RAATTY 6 cut(s) 502, 517, 610, 1261, 1564, 1906
XceI RCATGY 5 cut(s) 1386, 1689, 1993, 1997, 2108
XmiI GTMKAC 2 cut(s) 175, 536
XspI CTAG 1 cut(s) 527
Zsp2I ATGCAT 1 cut(s) 1995
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.