pycom03g13380

KH-domain-like of EngA bacterial GTPase enzymes, C-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0001492
Physical Location & Seq
Forward (+)
14394354 .. 14401473
7120 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g13380.1

Sequence Viewer

Length: 1917 bp
ATGGCAGCCCCCTCGAAGCTCTTGTGCTACAGAGGAACCACCACTTCTCCCTCTCTCTCCAAAACTCTCTCTCTCCTCTCTTCCCCATTTCCAATTCCCACATATTTCTCTCGCAGTCTCCGCACTCCTTCCCTTTCTCTCCACACTTCTCTTCAAACTCTTCCTCGCTCCGTTTCCCGCCTCCCTGCCAACAAATTCAAGATCTCCTCCACAGCAGATGACCAACAACAACAAGAAGGCGATGGCGAAGAGGAGGAGGAGCTCGTTGACGAGTCCGACGGCGGCGGCGGCGAGTTATTGACGGCCGACGTCGATGACCTGGAAGAAGACGCTCGATACTTAGTTGAAGAGTTCTCTTCCTCTTTGTCCACCCAACTCAGAATTGAAGATGACAAAGATGACCAAGAAAAAGTTGGTCGAAAGCAGAGGAATCAGAAAAGTTCAGTTAAAACTATCCCTGACCATCTTCTTCCAAGGGTTGCAATTGTTGGAAGACCGAATGTTGGCAAATCAGCATTATTTAATCGTCTTGTCTCGGGGAACAAGGCAATTGTAGTGGATGAGCCAGGGGTTACTAGGGATCGCCTGTATGGTAGATCGTTTTGGGGTGACCATGAATTTATGGTGGTGGATACTGGTGGTGTACTTACAGTTTCAAAGTCCCAGGCTAATGTCATGGAAGAATTAGCTATTTCAACAACCATTGGTATGGATGGAATCCCACTTGCCTCAAGGGAGGCAGCTGTTGCTAGGATGCCCTCAATGATAGAGAAACAAGCTACTGCAGCTGTTGAAGAATCATCTGTCATTATATTCCTCGTTGATGGCCAGGCAGGTCTAACTGCAGCAGATGAGGAAATTTCAGATTGGCTACGTAAAAACTTCTCAGATAAGTACATCATTCTTGCAGTTAACAAGTGTGAATCTCCACGTAAAGGATCGATCATGCAAGCATCAGAATTTTGGTCATTGGGGGTTGTTTTCACCACTTCCTATATCTGCTATATCTGGAACTGGAACTGGAGAGCTTCGATCGATCTTGTGTGTTCAGGGCTGAAAAAGAATGAGGACCCGGAGGATTTTGCTGAACAGGAAGAGTATGTTCCTGCAATTGCAATTGTTGGCCGACCAAATGTTGGTAAAAGTAGCATTTTGAATGCATTGGTTCGAGAGGACAGGACAATTGTTAGCCCAGTCAGTGGAACAACCCGTGATGCTATTGATATGGAATTTACTGGACCAGATGGACAGAAATTCAAACTAATTGATACTGCTGGAATCAGAAGAAGGGCCGTCGTTGCTTCATCAGGTAGCATGACAGAGGCTTTATCGGTAAATCGAGCATTTCGTGCCATTCGTCGTTCTGATGTTGTGGCTCTTGTCATTGAGGCCCTGGCTTGTATCACAGAACAGGATTGCAAGATTGCTGAGAGGATAGAAAGAGAAGGCAAAGGTTGCCTGATAGTTGTAAACAAATGGGATACCGTACCAAATAAAAACCAGCAGACTGCAACATACTATGAGCAGGATGTCAGGGAGAAGCTTCGTATCCTTGGTTGGGCACCTATTGTATATTCAACTGCAATAGCTGGTCAGAGTGTTGATAAGTACGAAGCACTAGCTTTTAAATCACCACCGAGGACACGAGGTGGCAAAAGAGGCCGCATTTATTACTGCACTCAGGCAGCTATAAGGCCGCCAACATTTGTGTTCTTTGTCAATGATTCAAAACTTTTCCCCGAGACATACCGGCGTTATATGGAGAAGCAACTCCGTTCAGATGCTGGGTTTTTAGGCACTCCAATTAGGCTTCTATGGTCTAACAGGAGAAAAACAGAAAAAGAGGAAGGAAGAGCTGCAATGCGAGCACAGGCAAATCTTGTGCCAAGTGACCGAAAATTGGAGTTAGCTACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KH_dom-like MMR_HSR1

Protein Analysis

639

Amino Acids

70.83

Weight (kDa)

6.02

Isoelectric Point (pI)

48.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MMR_HSR1 PF01926 159 - 306 1.8e-23 50S ribosome-binding GTPase
FeoB_N PF02421 159 - 221 2.6e-08 Ferrous iron transport protein B
MMR_HSR1 PF01926 371 - 492 1.8e-26 50S ribosome-binding GTPase
FeoB_N PF02421 371 - 502 2.5e-08 Ferrous iron transport protein B
KH_dom-like PF14714 538 - 606 8.4e-24 KH-domain-like of EngA bacterial GTPase enzymes, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 312
Acc36I ACCTGC 1 cut(s) 824
AccB1I GGYRCC 1 cut(s) 1561
AccB7I CCANNNNNTGG 2 cut(s) 1136, 1199
AciI CCGC 7 cut(s) 121, 178, 282, 285, 288, 1663, 1697
AclWI GGATC 2 cut(s) 588, 946
AcoI YGGCCR 3 cut(s) 303, 826, 1123
AcsI RAATTY 6 cut(s) 194, 617, 858, 959, 1229, 1253
AcyI GRCGYC 1 cut(s) 309
AdeI CACNNNGTG 1 cut(s) 1649
AfaI GTAC 4 cut(s) 645, 896, 1488, 1610
AfiI CCNNNNNNNGG 6 cut(s) 503, 935, 1136, 1199, 1558, 1900
AjnI CCWGG 5 cut(s) 318, 565, 663, 828, 1392
Alw21I GWGCWC 2 cut(s) 264, 1870
Alw26I GTCTC 3 cut(s) 122, 538, 1736
AlwI GGATC 2 cut(s) 588, 946
AlwNI CAGNNNCTG 1 cut(s) 1784
Ama87I CYCGRG 2 cut(s) 535, 1739
AoxI GGCC 7 cut(s) 303, 826, 1123, 1290, 1389, 1660, 1694
ApeKI GCWGC 6 cut(s) 5, 740, 785, 845, 1685, 1856
ApoI RAATTY 6 cut(s) 194, 617, 858, 959, 1229, 1253
AspS9I GGNCC 4 cut(s) 1069, 1238, 1290, 1390
AsuC2I CCSGG 1 cut(s) 1073
AsuHPI GGTGA 3 cut(s) 620, 976, 1623
AvaI CYCGRG 2 cut(s) 535, 1739
AvaII GGWCC 2 cut(s) 1069, 1238
BaeGI GKGCMC 1 cut(s) 1564
BalI TGGCCA 1 cut(s) 828
BanI GGYRCC 1 cut(s) 1561
BanII GRGCYC 1 cut(s) 264
BauI CACGAG 1 cut(s) 1644
BbsI GAAGAC 2 cut(s) 333, 499
Bbv12I GWGCWC 2 cut(s) 264, 1870
BbvI GCAGC 6 cut(s) 17, 752, 797, 857, 1697, 1843
BccI CCATC 5 cut(s) 236, 471, 707, 818, 1238
BceAI ACGGC 3 cut(s) 295, 318, 1277
BcgI CGANNNNNNTGC 1 cut(s) 28
BciT130I CCWGG 5 cut(s) 320, 567, 665, 830, 1394
BciVI GTATCC 3 cut(s) 625, 1474, 1559
BcnI CCSGG 1 cut(s) 1073
BcoDI GTCTC 3 cut(s) 122, 538, 1736
BfaI CTAG 3 cut(s) 576, 750, 1619
BfmI CTRYAG 3 cut(s) 28, 783, 843
BfuAI ACCTGC 1 cut(s) 824
BfuI GTATCC 3 cut(s) 625, 1474, 1559
BglII AGATCT 1 cut(s) 201
Bme1390I CCNGG 6 cut(s) 320, 567, 665, 830, 1073, 1394
Bme18I GGWCC 2 cut(s) 1069, 1238
BmeT110I CYCGRG 2 cut(s) 535, 1739
BmgT120I GGNCC 4 cut(s) 1069, 1238, 1290, 1390
BmiI GGNNCC 3 cut(s) 37, 1071, 1563
BmrFI CCNGG 6 cut(s) 320, 567, 665, 830, 1073, 1394
BmrI ACTGGG 1 cut(s) 1187
BmsI GCATC 4 cut(s) 744, 962, 1204, 1771
BmuI ACTGGG 1 cut(s) 1187
BpiI GAAGAC 2 cut(s) 333, 499
BpmI CTGGAG 1 cut(s) 1042
BpuEI CTTGAG 1 cut(s) 715
BpuMI CCSGG 1 cut(s) 1073
Bsa29I ATCGAT 2 cut(s) 941, 1035
BsaAI YACGTR 2 cut(s) 875, 932
BsaHI GRCGYC 1 cut(s) 309
BsaJI CCNNGG 6 cut(s) 473, 566, 663, 1392, 1552, 1637
BsaXI ACNNNNNCTCC 2 cut(s) 31, 61
Bsc4I CCNNNNNNNGG 6 cut(s) 503, 935, 1136, 1199, 1558, 1900
Bse118I RCCGGY 1 cut(s) 1749
Bse1I ACTGG 5 cut(s) 640, 1019, 1025, 1193, 1240
Bse3DI GCAATG 1 cut(s) 1866
BseBI CCWGG 5 cut(s) 320, 567, 665, 830, 1394
BseCI ATCGAT 2 cut(s) 941, 1035
BseDI CCNNGG 6 cut(s) 473, 566, 663, 1392, 1552, 1637
BseGI GGATG 4 cut(s) 565, 718, 759, 1534
BseLI CCNNNNNNNGG 6 cut(s) 503, 935, 1136, 1199, 1558, 1900
BseMI GCAATG 1 cut(s) 1866
BseMII CTCAG 4 cut(s) 391, 900, 1419, 1694
BseNI ACTGG 5 cut(s) 640, 1019, 1025, 1193, 1240
BseRI GAGGAG 5 cut(s) 65, 196, 266, 269, 272
BseSI GKGCMC 1 cut(s) 1564
BseX3I CGGCCG 1 cut(s) 303
BseXI GCAGC 6 cut(s) 17, 752, 797, 857, 1697, 1843
BseYI CCCAGC 1 cut(s) 1784
BsgI GTGCAG 1 cut(s) 1660
Bsh1285I CGRYCG 2 cut(s) 306, 1035
BshFI GGCC 7 cut(s) 305, 828, 1125, 1292, 1391, 1662, 1696
BshNI GGYRCC 1 cut(s) 1561
BshVI ATCGAT 2 cut(s) 941, 1035
BsiEI CGRYCG 2 cut(s) 306, 1035
BsiHKAI GWGCWC 2 cut(s) 264, 1870
BsiHKCI CYCGRG 2 cut(s) 535, 1739
BsiSI CCGG 2 cut(s) 1073, 1750
BslFI GGGAC 1 cut(s) 646
BslI CCNNNNNNNGG 6 cut(s) 503, 935, 1136, 1199, 1558, 1900
BsmAI GTCTC 3 cut(s) 122, 538, 1736
BsmFI GGGAC 1 cut(s) 646
BsmI GAATGC 1 cut(s) 1162
BsnI GGCC 7 cut(s) 305, 828, 1125, 1292, 1391, 1662, 1696
BsoBI CYCGRG 2 cut(s) 535, 1739
Bsp1286I GDGCHC 3 cut(s) 264, 1564, 1870
Bsp143I GATC 7 cut(s) 201, 580, 596, 938, 942, 1032, 1036
BspACI CCGC 7 cut(s) 121, 178, 282, 285, 288, 1663, 1697
BspANI GGCC 7 cut(s) 305, 828, 1125, 1292, 1391, 1662, 1696
BspCNI CTCAG 4 cut(s) 390, 899, 1420, 1693
BspDI ATCGAT 2 cut(s) 941, 1035
BspLI GGNNCC 3 cut(s) 37, 1071, 1563
BspMAI CTGCAG 2 cut(s) 787, 847
BspMI ACCTGC 1 cut(s) 824
BspPI GGATC 2 cut(s) 588, 946
BspQI GCTCTTC 1 cut(s) 1846
BspT107I GGYRCC 1 cut(s) 1561
BsrDI GCAATG 1 cut(s) 1866
BsrFI RCCGGY 1 cut(s) 1749
BsrI ACTGG 5 cut(s) 640, 1019, 1025, 1193, 1240
BssAI RCCGGY 1 cut(s) 1749
BssECI CCNNGG 6 cut(s) 473, 566, 663, 1392, 1552, 1637
BssMI GATC 7 cut(s) 201, 580, 596, 938, 942, 1032, 1036
BssNI GRCGYC 1 cut(s) 309
BssSI CACGAG 1 cut(s) 1644
BssT1I CCWWGG 2 cut(s) 473, 1552
Bst2BI CACGAG 1 cut(s) 1644
Bst2UI CCWGG 5 cut(s) 320, 567, 665, 830, 1394
Bst4CI ACNGT 2 cut(s) 652, 1486
Bst6I CTCTTC 8 cut(s) 85, 156, 165, 243, 342, 361, 1089, 1846
BstACI GRCGYC 1 cut(s) 309
BstAPI GCANNNNNTGC 3 cut(s) 746, 1349, 1455
BstBAI YACGTR 2 cut(s) 875, 932
BstC8I GCNNGC 2 cut(s) 951, 1866
BstDEI CTNAG 5 cut(s) 340, 377, 886, 1428, 1680
BstEII GGTNACC 1 cut(s) 608
BstF5I GGATG 4 cut(s) 565, 718, 759, 1534
BstKTI GATC 7 cut(s) 204, 583, 599, 941, 945, 1035, 1039
BstMAI GTCTC 3 cut(s) 122, 538, 1736
BstMBI GATC 7 cut(s) 201, 580, 596, 938, 942, 1032, 1036
BstMCI CGRYCG 2 cut(s) 306, 1035
BstMWI GCNNNNNNNGC 9 cut(s) 120, 288, 746, 785, 1298, 1349, 1455, 1659, 1865
BstNI CCWGG 5 cut(s) 320, 567, 665, 830, 1394
BstPI GGTNACC 1 cut(s) 608
BstSCI CCNGG 6 cut(s) 318, 565, 663, 828, 1071, 1392
BstSFI CTRYAG 3 cut(s) 28, 783, 843
BstSLI GKGCMC 1 cut(s) 1564
BstSNI TACGTA 1 cut(s) 875
BstV1I GCAGC 6 cut(s) 17, 752, 797, 857, 1697, 1843
BstV2I GAAGAC 2 cut(s) 333, 499
BstX2I RGATCY 1 cut(s) 201
BstXI CCANNNNNNTGG 1 cut(s) 709
BstYI RGATCY 1 cut(s) 201
BstZI CGGCCG 1 cut(s) 303
Bsu15I ATCGAT 2 cut(s) 941, 1035
BsuI GTATCC 3 cut(s) 625, 1474, 1559
BsuRI GGCC 7 cut(s) 305, 828, 1125, 1292, 1391, 1662, 1696
BsuTUI ATCGAT 2 cut(s) 941, 1035
BtgZI GCGATG 1 cut(s) 255
BtsCI GGATG 4 cut(s) 565, 718, 759, 1534
BtsIMutI CAGTG 1 cut(s) 1204
BveI ACCTGC 1 cut(s) 824
Cac8I GCNNGC 2 cut(s) 951, 1866
CaiI CAGNNNCTG 1 cut(s) 1784
Cfr10I RCCGGY 1 cut(s) 1749
Cfr13I GGNCC 4 cut(s) 1069, 1238, 1290, 1390
ClaI ATCGAT 2 cut(s) 941, 1035
CseI GACGC 1 cut(s) 338
Csp6I GTAC 4 cut(s) 644, 895, 1487, 1609
CspCI CAANNNNNGTGG 2 cut(s) 537, 572
CviAII CATG 5 cut(s) 614, 676, 946, 1315, 1914
CviQI GTAC 4 cut(s) 644, 895, 1487, 1609
DdeI CTNAG 5 cut(s) 340, 377, 886, 1428, 1680
DpnI GATC 7 cut(s) 203, 582, 598, 940, 944, 1034, 1038
DpnII GATC 7 cut(s) 201, 580, 596, 938, 942, 1032, 1036
DraI TTTAAA 1 cut(s) 1627
DraIII CACNNNGTG 1 cut(s) 1649
EaeI YGGCCR 3 cut(s) 303, 826, 1123
EagI CGGCCG 1 cut(s) 303
Eam1104I CTCTTC 8 cut(s) 85, 156, 165, 243, 342, 361, 1089, 1846
EarI CTCTTC 8 cut(s) 85, 156, 165, 243, 342, 361, 1089, 1846
Ecl136II GAGCTC 1 cut(s) 262
EclXI CGGCCG 1 cut(s) 303
Eco105I TACGTA 1 cut(s) 875
Eco130I CCWWGG 2 cut(s) 473, 1552
Eco24I GRGCYC 1 cut(s) 264
Eco47I GGWCC 2 cut(s) 1069, 1238
Eco52I CGGCCG 1 cut(s) 303
Eco53kI GAGCTC 1 cut(s) 262
Eco88I CYCGRG 2 cut(s) 535, 1739
Eco91I GGTNACC 1 cut(s) 608
EcoICRI GAGCTC 1 cut(s) 262
EcoO109I RGGNCCY 2 cut(s) 1069, 1390
EcoO65I GGTNACC 1 cut(s) 608
EcoRII CCWGG 5 cut(s) 318, 565, 663, 828, 1392
EcoT14I CCWWGG 2 cut(s) 473, 1552
EcoT22I ATGCAT 1 cut(s) 1162
EcoT38I GRGCYC 1 cut(s) 264
ErhI CCWWGG 2 cut(s) 473, 1552
FaeI CATG 5 cut(s) 617, 679, 949, 1318, 1917
FaqI GGGAC 1 cut(s) 646
FatI CATG 5 cut(s) 613, 675, 945, 1314, 1913
FauI CCCGC 1 cut(s) 185
FokI GGATG 4 cut(s) 572, 725, 766, 1541
FriOI GRGCYC 1 cut(s) 264
FspBI CTAG 3 cut(s) 576, 750, 1619
GsaI CCCAGC 1 cut(s) 1788
GsuI CTGGAG 1 cut(s) 1042
HaeIII GGCC 7 cut(s) 305, 828, 1125, 1292, 1391, 1662, 1696
HapII CCGG 2 cut(s) 1073, 1750
HgaI GACGC 1 cut(s) 338
Hin1I GRCGYC 1 cut(s) 309
Hin1II CATG 5 cut(s) 617, 679, 949, 1318, 1917
HincII GTYRAC 2 cut(s) 268, 913
HindII GTYRAC 2 cut(s) 268, 913
HindIII AAGCTT 1 cut(s) 1541
HinfI GANTC 7 cut(s) 272, 430, 717, 797, 923, 1278, 1724
HpaI GTTAAC 1 cut(s) 913
HpaII CCGG 2 cut(s) 1073, 1750
HphI GGTGA 3 cut(s) 620, 976, 1623
Hpy166II GTNNAC 5 cut(s) 268, 369, 644, 913, 1471
Hpy188III TCNNGA 3 cut(s) 199, 1009, 1169
Hpy8I GTNNAC 5 cut(s) 268, 369, 644, 913, 1471
Hpy99I CGWCG 5 cut(s) 281, 311, 314, 1298, 1362
HpyAV CCTTC 5 cut(s) 138, 230, 1281, 1439, 1841
HpyCH4III ACNGT 2 cut(s) 652, 1486
HpyCH4IV ACGT 3 cut(s) 309, 874, 931
HpyF10VI GCNNNNNNNGC 9 cut(s) 120, 288, 746, 785, 1298, 1349, 1455, 1659, 1865
HpyF3I CTNAG 5 cut(s) 340, 377, 886, 1428, 1680
HpySE526I ACGT 3 cut(s) 309, 874, 931
Hsp92I GRCGYC 1 cut(s) 309
Hsp92II CATG 5 cut(s) 617, 679, 949, 1318, 1917
KspAI GTTAAC 1 cut(s) 913
Kzo9I GATC 7 cut(s) 201, 580, 596, 938, 942, 1032, 1036
LguI GCTCTTC 1 cut(s) 1846
LmnI GCTCC 2 cut(s) 173, 259
Lsp1109I GCAGC 6 cut(s) 17, 752, 797, 857, 1697, 1843
LweI GCATC 4 cut(s) 744, 962, 1204, 1771
MaeI CTAG 3 cut(s) 576, 750, 1619
MaeII ACGT 3 cut(s) 309, 874, 931
MaeIII GTNAC 3 cut(s) 571, 608, 1889
MalI GATC 7 cut(s) 203, 582, 598, 940, 944, 1034, 1038
MboI GATC 7 cut(s) 201, 580, 596, 938, 942, 1032, 1036
MfeI CAATTG 5 cut(s) 483, 549, 1110, 1116, 1182
MflI RGATCY 1 cut(s) 201
MhlI GDGCHC 3 cut(s) 264, 1564, 1870
MlsI TGGCCA 1 cut(s) 828
MluNI TGGCCA 1 cut(s) 828
MlyI GAGTC 1 cut(s) 281
MmeI TCCRAC 2 cut(s) 300, 469
Mox20I TGGCCA 1 cut(s) 828
Mph1103I ATGCAT 1 cut(s) 1162
MscI TGGCCA 1 cut(s) 828
MseI TTAA 4 cut(s) 447, 522, 912, 1626
MslI CAYNNNNRTG 1 cut(s) 707
Msp20I TGGCCA 1 cut(s) 828
MspA1I CMGCKG 2 cut(s) 743, 788
MspI CCGG 2 cut(s) 1073, 1750
MspR9I CCNGG 6 cut(s) 320, 567, 665, 830, 1073, 1394
MunI CAATTG 5 cut(s) 483, 549, 1110, 1116, 1182
Mva1269I GAATGC 1 cut(s) 1162
MvaI CCWGG 5 cut(s) 320, 567, 665, 830, 1394
MwoI GCNNNNNNNGC 9 cut(s) 120, 288, 746, 785, 1298, 1349, 1455, 1659, 1865
NciI CCSGG 1 cut(s) 1073
NdeII GATC 7 cut(s) 201, 580, 596, 938, 942, 1032, 1036
NlaIII CATG 5 cut(s) 617, 679, 949, 1318, 1917
NlaIV GGNNCC 3 cut(s) 37, 1071, 1563
NmuCI GTSAC 2 cut(s) 608, 1889
NsiI ATGCAT 1 cut(s) 1162
PciSI GCTCTTC 1 cut(s) 1846
PcsI WCGNNNNNNNCGW 2 cut(s) 1345, 1354
PctI GAATGC 1 cut(s) 1162
PfeI GAWTC 6 cut(s) 430, 717, 797, 923, 1278, 1724
PflMI CCANNNNNTGG 2 cut(s) 1136, 1199
Ple19I CGATCG 1 cut(s) 1035
PleI GAGTC 1 cut(s) 280
PpsI GAGTC 1 cut(s) 280
Ppu21I YACGTR 2 cut(s) 875, 932
PpuMI RGGWCCY 1 cut(s) 1069
Psp124BI GAGCTC 1 cut(s) 264
Psp5II RGGWCCY 1 cut(s) 1069
Psp6I CCWGG 5 cut(s) 318, 565, 663, 828, 1392
PspEI GGTNACC 1 cut(s) 608
PspFI CCCAGC 1 cut(s) 1784
PspGI CCWGG 5 cut(s) 318, 565, 663, 828, 1392
PspN4I GGNNCC 3 cut(s) 37, 1071, 1563
PspPI GGNCC 4 cut(s) 1069, 1238, 1290, 1390
PspPPI RGGWCCY 1 cut(s) 1069
PstI CTGCAG 2 cut(s) 787, 847
PstNI CAGNNNCTG 1 cut(s) 1784
PsuI RGATCY 1 cut(s) 201
PvuI CGATCG 1 cut(s) 1035
PvuII CAGCTG 2 cut(s) 743, 788
RsaI GTAC 4 cut(s) 645, 896, 1488, 1610
RsaNI GTAC 4 cut(s) 644, 895, 1487, 1609
RseI CAYNNNNRTG 1 cut(s) 707
SacI GAGCTC 1 cut(s) 264
SapI GCTCTTC 1 cut(s) 1846
SaqAI TTAA 4 cut(s) 447, 522, 912, 1626
Sau3AI GATC 7 cut(s) 201, 580, 596, 938, 942, 1032, 1036
Sau96I GGNCC 4 cut(s) 1069, 1238, 1290, 1390
SchI GAGTC 1 cut(s) 281
ScrFI CCNGG 6 cut(s) 320, 567, 665, 830, 1073, 1394
SduI GDGCHC 3 cut(s) 264, 1564, 1870
SfaNI GCATC 4 cut(s) 744, 962, 1204, 1771
SfcI CTRYAG 3 cut(s) 28, 783, 843
SinI GGWCC 2 cut(s) 1069, 1238
SmiMI CAYNNNNRTG 1 cut(s) 707
SmlI CTYRAG 1 cut(s) 730
SmoI CTYRAG 1 cut(s) 730
SnaBI TACGTA 1 cut(s) 875
SsiI CCGC 7 cut(s) 121, 178, 282, 285, 288, 1663, 1697
SspMI CTAG 3 cut(s) 576, 750, 1619
SstI GAGCTC 1 cut(s) 264
StyD4I CCNGG 6 cut(s) 318, 565, 663, 828, 1071, 1392
StyI CCWWGG 2 cut(s) 473, 1552
TaaI ACNGT 2 cut(s) 652, 1486
TaiI ACGT 3 cut(s) 312, 877, 934
TaqI TCGA 9 cut(s) 14, 312, 334, 418, 941, 1031, 1035, 1168, 1339
TaqII GACCGA 2 cut(s) 511, 1908
TatI WGTACW 2 cut(s) 643, 894
TauI GCSGC 5 cut(s) 285, 288, 291, 1665, 1699
TfiI GAWTC 6 cut(s) 430, 717, 797, 923, 1278, 1724
Tru1I TTAA 4 cut(s) 447, 522, 912, 1626
Tru9I TTAA 4 cut(s) 447, 522, 912, 1626
TscAI CASTG 1 cut(s) 1204
TseFI GTSAC 2 cut(s) 608, 1889
TseI GCWGC 6 cut(s) 5, 740, 785, 845, 1685, 1856
Tsp45I GTSAC 2 cut(s) 608, 1889
TspDTI ATGAA 2 cut(s) 630, 1293
TspGWI ACGGA 2 cut(s) 160, 1763
TspRI CASTG 1 cut(s) 1204
Van91I CCANNNNNTGG 2 cut(s) 1136, 1199
VpaK11BI GGWCC 2 cut(s) 1069, 1238
XapI RAATTY 6 cut(s) 194, 617, 858, 959, 1229, 1253
XcmI CCANNNNNNNNNTGG 1 cut(s) 410
XspI CTAG 3 cut(s) 576, 750, 1619
ZraI GACGTC 1 cut(s) 310
Zsp2I ATGCAT 1 cut(s) 1162
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.