RchiOBHm_Chr3g0490621

KH-domain-like of EngA bacterial GTPase enzymes, C-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0001492
Physical Location & Seq
Forward (+)
37275969 .. 37285074
9106 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ45372

Sequence Viewer

Length: 1971 bp
ATGGCAGCAGCTGCTGAGCTCTTCTTGTCTTACAGAGGAGGAGGAACCACCGCTCTGTCCAAAACCCTATCTCTTGTCTCTTCCCCATTTCCCAAATCAATTCTTCATCTTCTCCGCACCACTCCCTCCCTTTCCCTTTCCCACCACCACTACCACCACCGCCACGCTTCTCGTCTTCCTCTCTCTCGCCTCTCCGCCACTGATCAAATCGATGTTTCCCTAACAGACGACGACGAAGAACAAGAACTAGAACCAGAGCCAGAGGGAGATGAGTCCGACGTCGATTTGGACTTCGACGCCCTCGAACAAGACGCCCTGGAACGAGTCCGAGACTTCTCTTCATCTCTCTCCAACCAGCTCACAATCGAAGATGAGAAAACTGATCAAGAGGCACTCGGCCGAAAGCAGAGGAGGCAGAAAACTCCAATCAAAAATAAAACTATCCCTGACCATCTTCTTCCGAGAGTTGCAATTGTTGGAAGACCAAATGTTGGTAAATCAGCATTATTCAATCGACTTGTCTCGGGGAACAAGGCAATTGTAGTTGATGAGCCAGGGGTCACTAGGGATCGCCTGTATGGTAGATCCTTTTGGGGGGACTTTGAATTTATGGTGGTCGATACTGGTGGTGTTCTTACAGTTTCAAAGTCACAGGCCAACGTCATGGAAGAACTGGCCATTTCAACAACCATTGGTATGGATGGAATTCCACTTGCCTCCAGAGAGGCAGCGGTTGCAAGGATGCCCTCAATGATTGAGAAACAAGCTACTGCAGCTGTTGAAGAGTCATCTGTCATTATTTTTCTTGTTGATGGCCAGGCTGGTCTAACTGCGGCTGACGAGGAAATTTCAGATTGGCTACGTAGGAACTTCTCAGATAAGTACATCATTCTTGCAGTCAACAAATGTGAATCTCCACGTAAAGGAGTCATGCAAGCATCAGAGTTTTGGTCCTTGGGGTTTTCGCCGCTTCCTATATCTGCTATATCTGGAACTGGAACCGGAGAGCTTCTCGATCTTGTGTGTTCAGGCCTAAAAAAGATTGAGGATCCTGATGATTTTGCTGAACAAGAAGATTATGTTCCTGCAATTGCAATTGTTGGTCGACCAAATGTTGGTAAAAGTAGCATTTTGAACGCGTTGGTTGGAGAAGACAGGACAATTGTTAGCCCAGTCAGTGGAACTACTCGTGATGCTATTGATATTGAATTCACTGGACCAGATGGACAGAAGTTCCGGCTTATTGACACTGCCGGAATCAGAAGAAAAGCCGTTGTTGCTTCATCAGGTAGTGTGACGGAGGCTTTATCAGTTAATCGAGCATTTCGTGCCATTCGCCGTTCTGATGTTGTGGCTCTTGTCATTGAGGCCATGGCTTGTATCACAGAACAGGATTGCAAGATTGCTGAAAGGATAGAAAGAGAAGGGAAAGGTTGCCTGATAGTTGTAAACAAATGGGACACTATACCAAATAAAAACCAGCAGACTGCAACATACTACGAGCAGGATGTGAGGGAAAAGCTTCGTATTCTCAATTGGGCGCCGGTTGTATATTCAACTGCACTAGCTGGTCGTAGTGTTGATAAAATTATCATGGCTGCTAGCACTGTTGAGAAAGAACGGTCAAGAAGGCTTACAACTGCTGTGGTAAATCAAGTGGTACAGGAGGCACTAGCTTTTAAATCACCACCTAGGACACGAGGTGGCAAAAGAGGACGTATTTATTACAGCACTCAGGCTGCTATAAGGCCACCAACATTTGTATTCTTTGTTAATGATGCGAAACTTTTCCCTGAAACTTATCGTCGCTATATGGAGAAGCAACTGCGTTCAGATGCTGGGTTTGCGGGTACTCCAATTCGCCTTTTATGGAGCAACAGGAGGAAGACAGAAAGAGATGAAGGGAGAGCTACAACAAGGACAAAAGGGAATCTTGTGCCACGTGACAAAAAATTGGAATTAGCTACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KH_dom-like MMR_HSR1

Protein Analysis

656

Amino Acids

72.45

Weight (kDa)

6.53

Isoelectric Point (pI)

46.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MMR_HSR1 PF01926 155 - 302 7.4e-23 50S ribosome-binding GTPase
FeoB_N PF02421 155 - 217 1.5e-07 Ferrous iron transport protein B
MMR_HSR1 PF01926 364 - 485 3.8e-27 50S ribosome-binding GTPase
FeoB_N PF02421 364 - 530 3.1e-09 Ferrous iron transport protein B
KH_dom-like PF14714 544 - 624 5.4e-28 KH-domain-like of EngA bacterial GTPase enzymes, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 282
AccB1I GGYRCC 1 cut(s) 1540
AccB7I CCANNNNNTGG 3 cut(s) 491, 1115, 1178
AccBSI CCGCTC 1 cut(s) 53
AccI GTMKAC 1 cut(s) 1105
AccII CGCG 1 cut(s) 1139
AciI CCGC 8 cut(s) 51, 115, 160, 195, 731, 833, 968, 1847
AclWI GGATC 4 cut(s) 576, 579, 1043, 1056
AcoI YGGCCR 3 cut(s) 397, 675, 814
AcsI RAATTY 4 cut(s) 605, 705, 846, 1208
AcvI CACGTG 1 cut(s) 1943
AcyI GRCGYC 4 cut(s) 279, 297, 312, 1541
AdeI CACNNNGTG 1 cut(s) 1703
AfaI GTAC 3 cut(s) 884, 1662, 1852
AfiI CCNNNNNNNGG 5 cut(s) 491, 594, 923, 1115, 1178
AflIII ACRYGT 1 cut(s) 1137
AgsI TTSAA 8 cut(s) 511, 605, 645, 684, 782, 1135, 1208, 1557
AjnI CCWGG 3 cut(s) 315, 553, 816
Alw21I GWGCWC 1 cut(s) 21
Alw26I GTCTC 3 cut(s) 82, 324, 526
AlwI GGATC 4 cut(s) 576, 579, 1043, 1056
AlwNI CAGNNNCTG 3 cut(s) 11, 14, 1838
Ama87I CYCGRG 1 cut(s) 523
AoxI GGCC 7 cut(s) 397, 654, 675, 814, 1030, 1368, 1748
ApeKI GCWGC 7 cut(s) 5, 8, 11, 728, 773, 1598, 1739
ApoI RAATTY 4 cut(s) 605, 705, 846, 1208
Asp700I GAANNNNTTC 2 cut(s) 1521, 1787
AspA2I CCTAGG 1 cut(s) 1691
AspLEI GCGC 1 cut(s) 1543
AspS9I GGNCC 2 cut(s) 951, 1217
AsuHPI GGTGA 1 cut(s) 1677
AsuNHI GCTAGC 1 cut(s) 1601
AvaI CYCGRG 1 cut(s) 523
AvaII GGWCC 2 cut(s) 951, 1217
AvrII CCTAGG 1 cut(s) 1691
BalI TGGCCA 2 cut(s) 677, 816
BamHI GGATCC 1 cut(s) 1048
BanI GGYRCC 1 cut(s) 1540
BanII GRGCYC 1 cut(s) 21
BauI CACGAG 2 cut(s) 1188, 1698
BbrPI CACGTG 1 cut(s) 1943
BbsI GAAGAC 4 cut(s) 167, 487, 1158, 1892
Bbv12I GWGCWC 1 cut(s) 21
BbvI GCAGC 6 cut(s) 17, 20, 740, 785, 1585, 1726
BccI CCATC 4 cut(s) 459, 695, 806, 1217
BceAI ACGGC 2 cut(s) 1256, 1323
BciT130I CCWGG 3 cut(s) 317, 555, 818
BclI TGATCA 2 cut(s) 202, 382
BcoDI GTCTC 3 cut(s) 82, 324, 526
BfaI CTAG 6 cut(s) 248, 564, 1565, 1602, 1673, 1692
BfmI CTRYAG 1 cut(s) 771
BfoI RGCGCY 1 cut(s) 1544
BisI GCNGC 9 cut(s) 6, 9, 12, 729, 774, 834, 968, 1599, 1740
BlnI CCTAGG 1 cut(s) 1691
BlpI GCTNAGC 1 cut(s) 15
BlsI GCNGC 9 cut(s) 7, 10, 13, 730, 775, 835, 969, 1600, 1741
Bme1390I CCNGG 3 cut(s) 317, 555, 818
Bme18I GGWCC 2 cut(s) 951, 1217
BmeT110I CYCGRG 1 cut(s) 523
BmgT120I GGNCC 2 cut(s) 951, 1217
BmiI GGNNCC 4 cut(s) 46, 1000, 1050, 1542
BmrFI CCNGG 3 cut(s) 317, 555, 818
BmrI ACTGGG 1 cut(s) 1166
BmsI GCATC 5 cut(s) 732, 947, 1183, 1768, 1825
BmtI GCTAGC 1 cut(s) 1605
BmuI ACTGGG 1 cut(s) 1166
BpiI GAAGAC 4 cut(s) 167, 487, 1158, 1892
BplI GAGNNNNNCTC 2 cut(s) 996, 1028
BpmI CTGGAG 1 cut(s) 703
Bpu1102I GCTNAGC 1 cut(s) 15
Bsa29I ATCGAT 1 cut(s) 210
BsaAI YACGTR 3 cut(s) 863, 920, 1943
BsaHI GRCGYC 4 cut(s) 279, 297, 312, 1541
BsaJI CCNNGG 5 cut(s) 315, 554, 954, 1371, 1691
BsaWI WCCGGW 1 cut(s) 1001
Bsc4I CCNNNNNNNGG 5 cut(s) 491, 594, 923, 1115, 1178
Bse118I RCCGGY 1 cut(s) 1543
Bse1I ACTGG 5 cut(s) 628, 678, 1000, 1172, 1219
BseBI CCWGG 3 cut(s) 317, 555, 818
BseCI ATCGAT 1 cut(s) 210
BseDI CCNNGG 5 cut(s) 315, 554, 954, 1371, 1691
BseGI GGATG 3 cut(s) 706, 747, 1513
BseLI CCNNNNNNNGG 5 cut(s) 491, 594, 923, 1115, 1178
BseMII CTCAG 3 cut(s) 6, 888, 1748
BseNI ACTGG 5 cut(s) 628, 678, 1000, 1172, 1219
BseRI GAGGAG 3 cut(s) 51, 54, 424
BseX3I CGGCCG 1 cut(s) 397
BseXI GCAGC 6 cut(s) 17, 20, 740, 785, 1585, 1726
BseYI CCCAGC 1 cut(s) 1838
BsgI GTGCAG 1 cut(s) 1545
Bsh1236I CGCG 1 cut(s) 1139
Bsh1285I CGRYCG 1 cut(s) 400
BshFI GGCC 7 cut(s) 399, 656, 677, 816, 1032, 1370, 1750
BshNI GGYRCC 1 cut(s) 1540
BshVI ATCGAT 1 cut(s) 210
BsiEI CGRYCG 1 cut(s) 400
BsiHKAI GWGCWC 1 cut(s) 21
BsiHKCI CYCGRG 1 cut(s) 523
BsiSI CCGG 4 cut(s) 1002, 1237, 1254, 1544
BslFI GGGAC 2 cut(s) 611, 1472
BslI CCNNNNNNNGG 5 cut(s) 491, 594, 923, 1115, 1178
BsmAI GTCTC 3 cut(s) 82, 324, 526
BsmFI GGGAC 2 cut(s) 611, 1472
BsnI GGCC 7 cut(s) 399, 656, 677, 816, 1032, 1370, 1750
BsoBI CYCGRG 1 cut(s) 523
Bsp1286I GDGCHC 1 cut(s) 21
Bsp143I GATC 6 cut(s) 202, 382, 568, 584, 1015, 1048
Bsp1720I GCTNAGC 1 cut(s) 15
Bsp19I CCATGG 1 cut(s) 1371
BspACI CCGC 8 cut(s) 51, 115, 160, 195, 731, 833, 968, 1847
BspANI GGCC 7 cut(s) 399, 656, 677, 816, 1032, 1370, 1750
BspCNI CTCAG 3 cut(s) 7, 887, 1747
BspDI ATCGAT 1 cut(s) 210
BspFNI CGCG 1 cut(s) 1139
BspLI GGNNCC 4 cut(s) 46, 1000, 1050, 1542
BspMAI CTGCAG 1 cut(s) 775
BspOI GCTAGC 1 cut(s) 1605
BspPI GGATC 4 cut(s) 576, 579, 1043, 1056
BspQI GCTCTTC 1 cut(s) 26
BspT107I GGYRCC 1 cut(s) 1540
BsrBI CCGCTC 1 cut(s) 53
BsrFI RCCGGY 1 cut(s) 1543
BsrI ACTGG 5 cut(s) 628, 678, 1000, 1172, 1219
BssAI RCCGGY 1 cut(s) 1543
BssECI CCNNGG 5 cut(s) 315, 554, 954, 1371, 1691
BssMI GATC 6 cut(s) 202, 382, 568, 584, 1015, 1048
BssNI GRCGYC 4 cut(s) 279, 297, 312, 1541
BssSI CACGAG 2 cut(s) 1188, 1698
BssT1I CCWWGG 3 cut(s) 954, 1371, 1691
Bst2BI CACGAG 2 cut(s) 1188, 1698
Bst2UI CCWGG 3 cut(s) 317, 555, 818
Bst4CI ACNGT 3 cut(s) 640, 1609, 1623
Bst6I CTCTTC 4 cut(s) 26, 85, 343, 777
BstACI GRCGYC 4 cut(s) 279, 297, 312, 1541
BstAPI GCANNNNNTGC 3 cut(s) 11, 734, 1328
BstBAI YACGTR 3 cut(s) 863, 920, 1943
BstC8I GCNNGC 2 cut(s) 936, 1603
BstDEI CTNAG 3 cut(s) 15, 874, 1734
BstDSI CCRYGG 1 cut(s) 1371
BstF5I GGATG 3 cut(s) 706, 747, 1513
BstFNI CGCG 1 cut(s) 1139
BstH2I RGCGCY 1 cut(s) 1544
BstHHI GCGC 1 cut(s) 1543
BstKTI GATC 6 cut(s) 205, 385, 571, 587, 1018, 1051
BstMAI GTCTC 3 cut(s) 82, 324, 526
BstMBI GATC 6 cut(s) 202, 382, 568, 584, 1015, 1048
BstMCI CGRYCG 1 cut(s) 400
BstMWI GCNNNNNNNGC 7 cut(s) 11, 412, 734, 773, 1277, 1328, 1844
BstNI CCWGG 3 cut(s) 317, 555, 818
BstSCI CCNGG 3 cut(s) 315, 553, 816
BstSFI CTRYAG 1 cut(s) 771
BstSNI TACGTA 1 cut(s) 863
BstUI CGCG 1 cut(s) 1139
BstV1I GCAGC 6 cut(s) 17, 20, 740, 785, 1585, 1726
BstV2I GAAGAC 4 cut(s) 167, 487, 1158, 1892
BstX2I RGATCY 2 cut(s) 584, 1048
BstXI CCANNNNNNTGG 2 cut(s) 664, 697
BstYI RGATCY 2 cut(s) 584, 1048
BstZI CGGCCG 1 cut(s) 397
Bsu15I ATCGAT 1 cut(s) 210
BsuRI GGCC 7 cut(s) 399, 656, 677, 816, 1032, 1370, 1750
BsuTUI ATCGAT 1 cut(s) 210
BtgI CCRYGG 1 cut(s) 1371
BtsCI GGATG 3 cut(s) 706, 747, 1513
BtsI GCAGTG 1 cut(s) 1248
BtsIMutI CAGTG 5 cut(s) 198, 1183, 1212, 1248, 1605
Cac8I GCNNGC 2 cut(s) 936, 1603
CaiI CAGNNNCTG 3 cut(s) 11, 14, 1838
CfoI GCGC 1 cut(s) 1543
Cfr10I RCCGGY 1 cut(s) 1543
Cfr13I GGNCC 2 cut(s) 951, 1217
ClaI ATCGAT 1 cut(s) 210
CseI GACGC 2 cut(s) 305, 320
Csp6I GTAC 3 cut(s) 883, 1661, 1851
CspCI CAANNNNNGTGG 2 cut(s) 1626, 1661
CviAII CATG 5 cut(s) 664, 931, 1372, 1594, 1968
CviQI GTAC 3 cut(s) 883, 1661, 1851
DdeI CTNAG 3 cut(s) 15, 874, 1734
DinI GGCGCC 1 cut(s) 1542
DpnI GATC 6 cut(s) 204, 384, 570, 586, 1017, 1050
DpnII GATC 6 cut(s) 202, 382, 568, 584, 1015, 1048
DraI TTTAAA 1 cut(s) 1681
DraIII CACNNNGTG 1 cut(s) 1703
EaeI YGGCCR 3 cut(s) 397, 675, 814
EagI CGGCCG 1 cut(s) 397
Eam1104I CTCTTC 4 cut(s) 26, 85, 343, 777
EarI CTCTTC 4 cut(s) 26, 85, 343, 777
EciI GGCGGA 1 cut(s) 184
Ecl136II GAGCTC 1 cut(s) 19
EclXI CGGCCG 1 cut(s) 397
Eco105I TACGTA 1 cut(s) 863
Eco130I CCWWGG 3 cut(s) 954, 1371, 1691
Eco147I AGGCCT 1 cut(s) 1032
Eco24I GRGCYC 1 cut(s) 21
Eco47I GGWCC 2 cut(s) 951, 1217
Eco52I CGGCCG 1 cut(s) 397
Eco53kI GAGCTC 1 cut(s) 19
Eco72I CACGTG 1 cut(s) 1943
Eco88I CYCGRG 1 cut(s) 523
EcoICRI GAGCTC 1 cut(s) 19
EcoRI GAATTC 2 cut(s) 705, 1208
EcoRII CCWGG 3 cut(s) 315, 553, 816
EcoT14I CCWWGG 3 cut(s) 954, 1371, 1691
EcoT38I GRGCYC 1 cut(s) 21
EgeI GGCGCC 1 cut(s) 1542
EheI GGCGCC 1 cut(s) 1542
ErhI CCWWGG 3 cut(s) 954, 1371, 1691
FaeI CATG 5 cut(s) 667, 934, 1375, 1597, 1971
FalI AAGNNNNNCTT 2 cut(s) 1917, 1949
FaqI GGGAC 2 cut(s) 611, 1472
FatI CATG 5 cut(s) 663, 930, 1371, 1593, 1967
FauI CCCGC 1 cut(s) 1840
FbaI TGATCA 2 cut(s) 202, 382
FblI GTMKAC 1 cut(s) 1105
Fnu4HI GCNGC 9 cut(s) 6, 9, 12, 729, 774, 834, 968, 1599, 1740
FokI GGATG 3 cut(s) 713, 754, 1520
FriOI GRGCYC 1 cut(s) 21
Fsp4HI GCNGC 9 cut(s) 6, 9, 12, 729, 774, 834, 968, 1599, 1740
FspBI CTAG 6 cut(s) 248, 564, 1565, 1602, 1673, 1692
GlaI GCGC 1 cut(s) 1542
GluI GCNGC 9 cut(s) 6, 9, 12, 729, 774, 834, 968, 1599, 1740
GsaI CCCAGC 1 cut(s) 1842
GsuI CTGGAG 1 cut(s) 703
HaeII RGCGCY 1 cut(s) 1544
HaeIII GGCC 7 cut(s) 399, 656, 677, 816, 1032, 1370, 1750
HapII CCGG 4 cut(s) 1002, 1237, 1254, 1544
HgaI GACGC 2 cut(s) 305, 320
HhaI GCGC 1 cut(s) 1543
Hin1I GRCGYC 4 cut(s) 279, 297, 312, 1541
Hin1II CATG 5 cut(s) 667, 934, 1375, 1597, 1971
Hin6I GCGC 1 cut(s) 1541
HinP1I GCGC 1 cut(s) 1541
HincII GTYRAC 2 cut(s) 901, 1106
HindII GTYRAC 2 cut(s) 901, 1106
HindIII AAGCTT 1 cut(s) 1520
HinfI GANTC 7 cut(s) 272, 324, 785, 911, 927, 1257, 1930
HpaII CCGG 4 cut(s) 1002, 1237, 1254, 1544
HphI GGTGA 1 cut(s) 1677
Hpy166II GTNNAC 3 cut(s) 901, 1106, 1450
Hpy188I TCNGA 9 cut(s) 277, 329, 462, 853, 877, 943, 1262, 1345, 1834
Hpy188III TCNNGA 7 cut(s) 386, 720, 990, 1013, 1052, 1190, 1626
Hpy8I GTNNAC 3 cut(s) 901, 1106, 1450
Hpy99I CGWCG 6 cut(s) 233, 236, 281, 284, 299, 1809
HpyAV CCTTC 3 cut(s) 1418, 1623, 1895
HpyCH4III ACNGT 3 cut(s) 640, 1609, 1623
HpyCH4IV ACGT 6 cut(s) 279, 660, 862, 919, 1717, 1942
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 412, 734, 773, 1277, 1328, 1844
HpyF3I CTNAG 3 cut(s) 15, 874, 1734
HpySE526I ACGT 6 cut(s) 279, 660, 862, 919, 1717, 1942
Hsp92I GRCGYC 4 cut(s) 279, 297, 312, 1541
Hsp92II CATG 5 cut(s) 667, 934, 1375, 1597, 1971
HspAI GCGC 1 cut(s) 1541
KasI GGCGCC 1 cut(s) 1540
Ksp22I TGATCA 2 cut(s) 202, 382
Kzo9I GATC 6 cut(s) 202, 382, 568, 584, 1015, 1048
LguI GCTCTTC 1 cut(s) 26
LmnI GCTCC 1 cut(s) 1872
Lsp1109I GCAGC 6 cut(s) 17, 20, 740, 785, 1585, 1726
LweI GCATC 5 cut(s) 732, 947, 1183, 1768, 1825
MaeI CTAG 6 cut(s) 248, 564, 1565, 1602, 1673, 1692
MaeII ACGT 6 cut(s) 279, 660, 862, 919, 1717, 1942
MaeIII GTNAC 4 cut(s) 559, 648, 1294, 1943
MalI GATC 6 cut(s) 204, 384, 570, 586, 1017, 1050
MbiI CCGCTC 1 cut(s) 53
MboI GATC 6 cut(s) 202, 382, 568, 584, 1015, 1048
MfeI CAATTG 6 cut(s) 471, 537, 1089, 1095, 1161, 1534
MflI RGATCY 2 cut(s) 584, 1048
MhlI GDGCHC 1 cut(s) 21
MlsI TGGCCA 2 cut(s) 677, 816
MluI ACGCGT 1 cut(s) 1137
MluNI TGGCCA 2 cut(s) 677, 816
Mly113I GGCGCC 1 cut(s) 1541
MlyI GAGTC 4 cut(s) 281, 333, 794, 936
MmeI TCCRAC 4 cut(s) 300, 375, 457, 1126
Mox20I TGGCCA 2 cut(s) 677, 816
MroXI GAANNNNTTC 2 cut(s) 1521, 1787
MscI TGGCCA 2 cut(s) 677, 816
MseI TTAA 3 cut(s) 1314, 1680, 1773
MslI CAYNNNNRTG 1 cut(s) 695
Msp20I TGGCCA 2 cut(s) 677, 816
MspA1I CMGCKG 3 cut(s) 11, 731, 776
MspI CCGG 4 cut(s) 1002, 1237, 1254, 1544
MspR9I CCNGG 3 cut(s) 317, 555, 818
MunI CAATTG 6 cut(s) 471, 537, 1089, 1095, 1161, 1534
MvaI CCWGG 3 cut(s) 317, 555, 818
MvnI CGCG 1 cut(s) 1139
MwoI GCNNNNNNNGC 7 cut(s) 11, 412, 734, 773, 1277, 1328, 1844
NarI GGCGCC 1 cut(s) 1541
NcoI CCATGG 1 cut(s) 1371
NdeII GATC 6 cut(s) 202, 382, 568, 584, 1015, 1048
NheI GCTAGC 1 cut(s) 1601
NlaIII CATG 5 cut(s) 667, 934, 1375, 1597, 1971
NlaIV GGNNCC 4 cut(s) 46, 1000, 1050, 1542
NmeAIII GCCGAG 1 cut(s) 375
NmuCI GTSAC 4 cut(s) 559, 648, 1294, 1943
PceI AGGCCT 1 cut(s) 1032
PciSI GCTCTTC 1 cut(s) 26
PcsI WCGNNNNNNNCGW 2 cut(s) 300, 1324
PdmI GAANNNNTTC 2 cut(s) 1521, 1787
PfeI GAWTC 3 cut(s) 911, 1257, 1930
PflMI CCANNNNNTGG 3 cut(s) 491, 1115, 1178
PkrI GCNGC 9 cut(s) 7, 10, 13, 730, 775, 835, 969, 1600, 1741
PleI GAGTC 4 cut(s) 280, 332, 793, 935
PluTI GGCGCC 1 cut(s) 1544
PmaCI CACGTG 1 cut(s) 1943
PmlI CACGTG 1 cut(s) 1943
PpsI GAGTC 4 cut(s) 280, 332, 793, 935
Ppu21I YACGTR 3 cut(s) 863, 920, 1943
Psp124BI GAGCTC 1 cut(s) 21
Psp6I CCWGG 3 cut(s) 315, 553, 816
PspCI CACGTG 1 cut(s) 1943
PspFI CCCAGC 1 cut(s) 1838
PspGI CCWGG 3 cut(s) 315, 553, 816
PspN4I GGNNCC 4 cut(s) 46, 1000, 1050, 1542
PspPI GGNCC 2 cut(s) 951, 1217
PstI CTGCAG 1 cut(s) 775
PstNI CAGNNNCTG 3 cut(s) 11, 14, 1838
PsuI RGATCY 2 cut(s) 584, 1048
PvuII CAGCTG 2 cut(s) 11, 776
RsaI GTAC 3 cut(s) 884, 1662, 1852
RsaNI GTAC 3 cut(s) 883, 1661, 1851
RseI CAYNNNNRTG 1 cut(s) 695
SacI GAGCTC 1 cut(s) 21
SalI GTCGAC 1 cut(s) 1104
SapI GCTCTTC 1 cut(s) 26
SaqAI TTAA 3 cut(s) 1314, 1680, 1773
SatI GCNGC 9 cut(s) 6, 9, 12, 729, 774, 834, 968, 1599, 1740
Sau3AI GATC 6 cut(s) 202, 382, 568, 584, 1015, 1048
Sau96I GGNCC 2 cut(s) 951, 1217
SchI GAGTC 4 cut(s) 281, 333, 794, 936
ScrFI CCNGG 3 cut(s) 317, 555, 818
SduI GDGCHC 1 cut(s) 21
SfaNI GCATC 5 cut(s) 732, 947, 1183, 1768, 1825
SfcI CTRYAG 1 cut(s) 771
SfoI GGCGCC 1 cut(s) 1542
SinI GGWCC 2 cut(s) 951, 1217
SmiMI CAYNNNNRTG 1 cut(s) 695
SnaBI TACGTA 1 cut(s) 863
SseBI AGGCCT 1 cut(s) 1032
SsiI CCGC 8 cut(s) 51, 115, 160, 195, 731, 833, 968, 1847
SspDI GGCGCC 1 cut(s) 1540
SspMI CTAG 6 cut(s) 248, 564, 1565, 1602, 1673, 1692
SstI GAGCTC 1 cut(s) 21
StuI AGGCCT 1 cut(s) 1032
StyD4I CCNGG 3 cut(s) 315, 553, 816
StyI CCWWGG 3 cut(s) 954, 1371, 1691
TaaI ACNGT 3 cut(s) 640, 1609, 1623
TaiI ACGT 6 cut(s) 282, 663, 865, 922, 1720, 1945
TatI WGTACW 1 cut(s) 882
TauI GCSGC 2 cut(s) 836, 970
TfiI GAWTC 3 cut(s) 911, 1257, 1930
Tru1I TTAA 3 cut(s) 1314, 1680, 1773
Tru9I TTAA 3 cut(s) 1314, 1680, 1773
TscAI CASTG 5 cut(s) 205, 1183, 1219, 1255, 1612
TseFI GTSAC 4 cut(s) 559, 648, 1294, 1943
TseI GCWGC 7 cut(s) 5, 8, 11, 728, 773, 1598, 1739
Tsp45I GTSAC 4 cut(s) 559, 648, 1294, 1943
TspDTI ATGAA 4 cut(s) 95, 330, 1272, 1914
TspGWI ACGGA 1 cut(s) 1313
TspRI CASTG 5 cut(s) 205, 1183, 1219, 1255, 1612
Van91I CCANNNNNTGG 3 cut(s) 491, 1115, 1178
VpaK11BI GGWCC 2 cut(s) 951, 1217
XapI RAATTY 4 cut(s) 605, 705, 846, 1208
XmaJI CCTAGG 1 cut(s) 1691
XmiI GTMKAC 1 cut(s) 1105
XmnI GAANNNNTTC 2 cut(s) 1521, 1787
XspI CTAG 6 cut(s) 248, 564, 1565, 1602, 1673, 1692
ZraI GACGTC 1 cut(s) 280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.