AT3G12300

Protein of unknown function (DUF667)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0014497
Physical Location & Seq
Reverse (-)
3921419 .. 3923155
1737 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G12300.1

Sequence Viewer

Length: 573 bp
ATGTTCAAGAACACGTTTCAATCTGGGTTTCTGTCTATTTTGTACAGTCTAGGAAGTAAGCCTCTTCAGATATGGGATAAAGAAGTTGTGGATGGACATGTGAAGCGTTGCCATGATGATGACATTCAATCCAATGTGCTTGAAGTAGTTGGATCAAATATTCAGTCTACATACATTACATGTCCTGCTGATCTTTCCGCTACTCTTGGTATCAAACTTCCTTTTTTGGTCTTGGTGGTGAAGGATATGAAGAAATATTTCTCATTCGAGATTCAGATTCTGGATGACAAGAATGTTCGCCGGCGTTTCCGAGCTTCTAACTTTCAAGCTGTCACTAGAGTAAAGCCGTATATTTGCACAATGCCACTGAAGATGGACGAGGGATGGAATCAAATCCAGCTTAACTTAGCGGATCTTACTAGGAGAGCTTATGGGACTAATTATGCTGAGACATTGCGAGTTCAGATTCATGCTAACTGCCGCCTTAGGCGCATATATTTTGCTGATCGTCTCTACTCTGAAGAGGAGCTTCCTCCGGAATTCAAGCTGTATCTCCCAGTGCAGAAAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF667

Protein Analysis

190

Amino Acids

22.1

Weight (kDa)

9.0

Isoelectric Point (pI)

51.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CFA20_dom PF05018 1 - 184 1.5e-89 CFA20 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014497)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12300
fragaria_vesca FvH4_7g03830
malus_domestica MD02G1276800.v1.1 MD07G1044300.v1.1
prunus_persica Prupe.2G043300_v2.0.a1 Prupe.2G043300_v2.0.a1
pyrus_communis pycom02g23710
rosa_chinensis RchiOBHm_Chr1g0327201
rosa_laevigata RLG00000030035
rosa_multiflora Rmu_sc0000972.1_g000014
rosa_roxburghii Rroxscaffold_4G00323070 Rroxscaffold_4G00323150
rosa_rugosa Rorug01G0064500
rosa_samantha Rh1AG081600 Rh1BG064800 Rh1DG086100
rosa_wichuraiana Rw1G006440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 167
AccIII TCCGGA 1 cut(s) 535
AciI CCGC 3 cut(s) 198, 410, 481
AclWI GGATC 2 cut(s) 160, 420
AcsI RAATTY 1 cut(s) 539
AcuI CTGAAG 3 cut(s) 50, 389, 540
AfaI GTAC 1 cut(s) 44
AflIII ACRYGT 3 cut(s) 12, 97, 179
AgsI TTSAA 6 cut(s) 7, 20, 128, 143, 326, 544
AluBI AGCT 6 cut(s) 314, 329, 400, 428, 529, 547
AluI AGCT 6 cut(s) 314, 329, 400, 428, 529, 547
Alw26I GTCTC 2 cut(s) 443, 515
AlwI GGATC 2 cut(s) 160, 420
AlwNI CAGNNNCTG 1 cut(s) 280
Aor13HI TCCGGA 1 cut(s) 535
ApoI RAATTY 1 cut(s) 539
ArsI GACNNNNNNTTYG 2 cut(s) 149, 181
Asp700I GAANNNNTTC 1 cut(s) 257
AspLEI GCGC 1 cut(s) 492
AsuHPI GGTGA 1 cut(s) 250
AxyI CCTNAGG 1 cut(s) 485
BccI CCATC 3 cut(s) 86, 367, 378
BceAI ACGGC 1 cut(s) 331
BcoDI GTCTC 2 cut(s) 443, 515
BfaI CTAG 3 cut(s) 50, 336, 420
BisI GCNGC 1 cut(s) 481
BlsI GCNGC 1 cut(s) 482
BmrI ACTGGG 1 cut(s) 551
BmuI ACTGGG 1 cut(s) 551
BsaWI WCCGGW 1 cut(s) 535
Bse118I RCCGGY 1 cut(s) 300
Bse1I ACTGG 1 cut(s) 557
Bse21I CCTNAGG 1 cut(s) 485
Bse3DI GCAATG 1 cut(s) 452
BseAI TCCGGA 1 cut(s) 535
BseGI GGATG 3 cut(s) 97, 289, 389
BseMI GCAATG 1 cut(s) 452
BseMII CTCAG 1 cut(s) 438
BseNI ACTGG 1 cut(s) 557
BseRI GAGGAG 1 cut(s) 539
BsiSI CCGG 2 cut(s) 301, 536
BslFI GGGAC 1 cut(s) 448
BsmAI GTCTC 2 cut(s) 443, 515
BsmBI CGTCTC 1 cut(s) 515
BsmFI GGGAC 1 cut(s) 448
Bsp13I TCCGGA 1 cut(s) 535
Bsp1407I TGTACA 1 cut(s) 42
Bsp143I GATC 4 cut(s) 152, 190, 412, 505
BspACI CCGC 3 cut(s) 198, 410, 481
BspCNI CTCAG 1 cut(s) 439
BspEI TCCGGA 1 cut(s) 535
BspPI GGATC 2 cut(s) 160, 420
BsrDI GCAATG 1 cut(s) 452
BsrFI RCCGGY 1 cut(s) 300
BsrGI TGTACA 1 cut(s) 42
BsrI ACTGG 1 cut(s) 557
BssAI RCCGGY 1 cut(s) 300
BssMI GATC 4 cut(s) 152, 190, 412, 505
Bst4CI ACNGT 1 cut(s) 47
Bst6I CTCTTC 2 cut(s) 69, 516
BstAUI TGTACA 1 cut(s) 42
BstC8I GCNNGC 1 cut(s) 302
BstDEI CTNAG 3 cut(s) 406, 447, 485
BstF5I GGATG 3 cut(s) 97, 289, 389
BstHHI GCGC 1 cut(s) 492
BstKTI GATC 4 cut(s) 155, 193, 415, 508
BstMAI GTCTC 2 cut(s) 443, 515
BstMBI GATC 4 cut(s) 152, 190, 412, 505
BstMWI GCNNNNNNNGC 1 cut(s) 489
BstNSI RCATGY 2 cut(s) 101, 183
BstX2I RGATCY 1 cut(s) 412
BstYI RGATCY 1 cut(s) 412
Bsu36I CCTNAGG 1 cut(s) 485
BtsCI GGATG 3 cut(s) 97, 289, 389
BtsIMutI CAGTG 2 cut(s) 365, 564
Cac8I GCNNGC 1 cut(s) 302
CaiI CAGNNNCTG 1 cut(s) 280
CfoI GCGC 1 cut(s) 492
Cfr10I RCCGGY 1 cut(s) 300
Csp6I GTAC 1 cut(s) 43
CviAII CATG 5 cut(s) 98, 113, 180, 470, 570
CviJI RGCY 8 cut(s) 61, 314, 329, 346, 400, 428, 529, 547
CviKI_1 RGCY 8 cut(s) 61, 314, 329, 346, 400, 428, 529, 547
CviQI GTAC 1 cut(s) 43
DdeI CTNAG 3 cut(s) 406, 447, 485
DpnI GATC 4 cut(s) 154, 192, 414, 507
DpnII GATC 4 cut(s) 152, 190, 412, 505
Eam1104I CTCTTC 2 cut(s) 69, 516
EarI CTCTTC 2 cut(s) 69, 516
Eco57I CTGAAG 3 cut(s) 50, 389, 540
Eco81I CCTNAGG 1 cut(s) 485
EcoRI GAATTC 1 cut(s) 539
Esp3I CGTCTC 1 cut(s) 515
FaeI CATG 5 cut(s) 101, 116, 183, 473, 573
FalI AAGNNNNNCTT 2 cut(s) 513, 545
FaqI GGGAC 1 cut(s) 448
FatI CATG 5 cut(s) 97, 112, 179, 469, 569
FblI GTMKAC 1 cut(s) 167
Fnu4HI GCNGC 1 cut(s) 481
FokI GGATG 3 cut(s) 104, 296, 396
Fsp4HI GCNGC 1 cut(s) 481
FspBI CTAG 3 cut(s) 50, 336, 420
GlaI GCGC 1 cut(s) 491
GluI GCNGC 1 cut(s) 481
HapII CCGG 2 cut(s) 301, 536
HhaI GCGC 1 cut(s) 492
Hin1II CATG 5 cut(s) 101, 116, 183, 473, 573
Hin6I GCGC 1 cut(s) 490
HinP1I GCGC 1 cut(s) 490
HinfI GANTC 4 cut(s) 271, 277, 388, 466
HpaII CCGG 2 cut(s) 301, 536
HphI GGTGA 1 cut(s) 250
Hpy166II GTNNAC 1 cut(s) 168
Hpy188I TCNGA 5 cut(s) 69, 276, 311, 465, 520
Hpy188III TCNNGA 4 cut(s) 7, 268, 281, 536
Hpy8I GTNNAC 1 cut(s) 168
HpyAV CCTTC 1 cut(s) 235
HpyCH4III ACNGT 1 cut(s) 47
HpyCH4IV ACGT 1 cut(s) 14
HpyCH4V TGCA 2 cut(s) 357, 562
HpyF10VI GCNNNNNNNGC 1 cut(s) 489
HpyF3I CTNAG 3 cut(s) 406, 447, 485
HpySE526I ACGT 1 cut(s) 14
Hsp92II CATG 5 cut(s) 101, 116, 183, 473, 573
HspAI GCGC 1 cut(s) 490
Kpn2I TCCGGA 1 cut(s) 535
KroI GCCGGC 1 cut(s) 300
KroNI GCCGGC 1 cut(s) 302
Kzo9I GATC 4 cut(s) 152, 190, 412, 505
LmnI GCTCC 1 cut(s) 526
LpnPI CCDG 6 cut(s) 9, 198, 266, 314, 410, 549
MaeI CTAG 3 cut(s) 50, 336, 420
MaeII ACGT 1 cut(s) 14
MaeIII GTNAC 1 cut(s) 331
MalI GATC 4 cut(s) 154, 192, 414, 507
MboI GATC 4 cut(s) 152, 190, 412, 505
MboII GAAGA 4 cut(s) 56, 262, 382, 533
MflI RGATCY 1 cut(s) 412
MluCI AATT 2 cut(s) 439, 539
MmeI TCCRAC 1 cut(s) 130
MnlI CCTC 4 cut(s) 72, 373, 517, 543
MreI CGCCGGCG 1 cut(s) 300
MroI TCCGGA 1 cut(s) 535
MroNI GCCGGC 1 cut(s) 300
MroXI GAANNNNTTC 1 cut(s) 257
MseI TTAA 1 cut(s) 402
MslI CAYNNNNRTG 1 cut(s) 117
MspI CCGG 2 cut(s) 301, 536
MwoI GCNNNNNNNGC 1 cut(s) 489
NaeI GCCGGC 1 cut(s) 302
NdeII GATC 4 cut(s) 152, 190, 412, 505
NgoMIV GCCGGC 1 cut(s) 300
NlaIII CATG 5 cut(s) 101, 116, 183, 473, 573
NmuCI GTSAC 1 cut(s) 331
NspI RCATGY 2 cut(s) 101, 183
PciI ACATGT 2 cut(s) 97, 179
PdiI GCCGGC 1 cut(s) 302
PdmI GAANNNNTTC 1 cut(s) 257
PfeI GAWTC 4 cut(s) 271, 277, 388, 466
PkrI GCNGC 1 cut(s) 482
PscI ACATGT 2 cut(s) 97, 179
PstNI CAGNNNCTG 1 cut(s) 280
PsuI RGATCY 1 cut(s) 412
RsaI GTAC 1 cut(s) 44
RsaNI GTAC 1 cut(s) 43
RseI CAYNNNNRTG 1 cut(s) 117
SaqAI TTAA 1 cut(s) 402
SatI GCNGC 1 cut(s) 481
Sau3AI GATC 4 cut(s) 152, 190, 412, 505
SetI ASST 7 cut(s) 17, 316, 331, 402, 430, 531, 549
SgrAI CRCCGGYG 1 cut(s) 300
SmiMI CAYNNNNRTG 1 cut(s) 117
Sse9I AATT 2 cut(s) 439, 539
SsiI CCGC 3 cut(s) 198, 410, 481
SspI AATATT 2 cut(s) 160, 257
SspMI CTAG 3 cut(s) 50, 336, 420
TaaI ACNGT 1 cut(s) 47
TaiI ACGT 1 cut(s) 17
TaqI TCGA 1 cut(s) 267
TasI AATT 2 cut(s) 439, 539
TatI WGTACW 1 cut(s) 42
TauI GCSGC 1 cut(s) 483
TfiI GAWTC 4 cut(s) 271, 277, 388, 466
Tru1I TTAA 1 cut(s) 402
Tru9I TTAA 1 cut(s) 402
TscAI CASTG 2 cut(s) 372, 564
TseFI GTSAC 1 cut(s) 331
Tsp45I GTSAC 1 cut(s) 331
TspDTI ATGAA 2 cut(s) 263, 458
TspRI CASTG 2 cut(s) 372, 564
XapI RAATTY 1 cut(s) 539
XceI RCATGY 2 cut(s) 101, 183
XmiI GTMKAC 1 cut(s) 167
XmnI GAANNNNTTC 1 cut(s) 257
XspI CTAG 3 cut(s) 50, 336, 420
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.