Rroxscaffold_4G00323150

Protein of unknown function (DUF667)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0014497
Physical Location & Seq
Forward (+)
53760106 .. 53762710
2605 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_4G00323150.1

Sequence Viewer

Length: 297 bp
ATGATAAGAATGTCAGGCGACGTTTTCGAGCTTCCAATTTTCAAGTGTGCTGTCACTCGAGTGAAGCCGTATATCTGCACCATGCCACTGAGGATGGATGAGGGCTGGAATCAAATCCAGTTTAATCTGGCTGATTTTACCAGGAGAGCATATGGAACAAACTATGTGGAGACATTGCGAGTTCAGGTTCATGCAAATTGTCGCCTGAGGAGGATATATTTCTCTGACCGCTTGTACTCTGAAGAGGAACTTCCTCCTGAATTTAAACTTTACCTTCCAATGCAGCAGAGGACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF667

Protein Analysis

98

Amino Acids

11.82

Weight (kDa)

8.9

Isoelectric Point (pI)

46.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CFA20_dom PF05018 18 - 91 3.8e-35 CFA20 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014497)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12300
fragaria_vesca FvH4_7g03830
malus_domestica MD02G1276800.v1.1 MD07G1044300.v1.1
prunus_persica Prupe.2G043300_v2.0.a1 Prupe.2G043300_v2.0.a1
pyrus_communis pycom02g23710
rosa_chinensis RchiOBHm_Chr1g0327201
rosa_laevigata RLG00000030035
rosa_multiflora Rmu_sc0000972.1_g000014
rosa_roxburghii Rroxscaffold_4G00323070 Rroxscaffold_4G00323150
rosa_rugosa Rorug01G0064500
rosa_samantha Rh1AG081600 Rh1BG064800 Rh1DG086100
rosa_wichuraiana Rw1G006440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 229
AcsI RAATTY 1 cut(s) 260
AcuI CTGAAG 1 cut(s) 261
AfaI GTAC 1 cut(s) 236
AgsI TTSAA 1 cut(s) 43
AjnI CCWGG 1 cut(s) 140
AleI CACNNNNGTG 1 cut(s) 59
AluBI AGCT 1 cut(s) 31
AluI AGCT 1 cut(s) 31
Alw26I GTCTC 1 cut(s) 164
Ama87I CYCGRG 1 cut(s) 57
ApeKI GCWGC 1 cut(s) 283
ApoI RAATTY 1 cut(s) 260
AvaI CYCGRG 1 cut(s) 57
AxyI CCTNAGG 1 cut(s) 206
BccI CCATC 1 cut(s) 88
BceAI ACGGC 1 cut(s) 52
BciT130I CCWGG 1 cut(s) 142
BcoDI GTCTC 1 cut(s) 164
BisI GCNGC 1 cut(s) 284
BlsI GCNGC 1 cut(s) 285
Bme1390I CCNGG 1 cut(s) 142
BmeT110I CYCGRG 1 cut(s) 57
BmrFI CCNGG 1 cut(s) 142
Bse1I ACTGG 1 cut(s) 118
Bse21I CCTNAGG 1 cut(s) 206
Bse3DI GCAATG 1 cut(s) 173
BseBI CCWGG 1 cut(s) 142
BseGI GGATG 2 cut(s) 99, 103
BseMI GCAATG 1 cut(s) 173
BseMII CTCAG 2 cut(s) 80, 197
BseNI ACTGG 1 cut(s) 118
BseRI GAGGAG 1 cut(s) 223
BsgI GTGCAG 1 cut(s) 61
BsiHKCI CYCGRG 1 cut(s) 57
BsmAI GTCTC 1 cut(s) 164
BsoBI CYCGRG 1 cut(s) 57
BspACI CCGC 1 cut(s) 229
BspCNI CTCAG 2 cut(s) 81, 198
BsrDI GCAATG 1 cut(s) 173
BsrI ACTGG 1 cut(s) 118
Bst2UI CCWGG 1 cut(s) 142
Bst6I CTCTTC 1 cut(s) 237
BstDEI CTNAG 2 cut(s) 89, 206
BstF5I GGATG 2 cut(s) 99, 103
BstMAI GTCTC 1 cut(s) 164
BstNI CCWGG 1 cut(s) 142
BstSCI CCNGG 1 cut(s) 140
Bsu36I CCTNAGG 1 cut(s) 206
BtsCI GGATG 2 cut(s) 99, 103
BtsIMutI CAGTG 1 cut(s) 86
Csp6I GTAC 1 cut(s) 235
CspCI CAANNNNNGTGG 2 cut(s) 147, 182
CviAII CATG 3 cut(s) 82, 191, 294
CviJI RGCY 4 cut(s) 31, 67, 105, 131
CviKI_1 RGCY 4 cut(s) 31, 67, 105, 131
CviQI GTAC 1 cut(s) 235
DdeI CTNAG 2 cut(s) 89, 206
DraI TTTAAA 1 cut(s) 265
Eam1104I CTCTTC 1 cut(s) 237
EarI CTCTTC 1 cut(s) 237
Eco57I CTGAAG 1 cut(s) 261
Eco81I CCTNAGG 1 cut(s) 206
Eco88I CYCGRG 1 cut(s) 57
EcoRII CCWGG 1 cut(s) 140
FaeI CATG 3 cut(s) 85, 194, 297
FaiI YATR 8 cut(s) 72, 83, 151, 153, 165, 192, 217, 295
FalI AAGNNNNNCTT 2 cut(s) 234, 266
FatI CATG 3 cut(s) 81, 190, 293
FauNDI CATATG 1 cut(s) 151
Fnu4HI GCNGC 1 cut(s) 284
FokI GGATG 2 cut(s) 106, 110
Fsp4HI GCNGC 1 cut(s) 284
GluI GCNGC 1 cut(s) 284
Hin1II CATG 3 cut(s) 85, 194, 297
HinfI GANTC 1 cut(s) 109
Hpy188I TCNGA 2 cut(s) 226, 241
Hpy188III TCNNGA 1 cut(s) 257
Hpy99I CGWCG 1 cut(s) 23
HpyAV CCTTC 1 cut(s) 284
HpyCH4IV ACGT 1 cut(s) 21
HpyCH4V TGCA 3 cut(s) 78, 194, 283
HpyF3I CTNAG 2 cut(s) 89, 206
HpySE526I ACGT 1 cut(s) 21
Hsp92II CATG 3 cut(s) 85, 194, 297
LpnPI CCDG 8 cut(s) 91, 113, 127, 131, 154, 170, 218, 270
MaeII ACGT 1 cut(s) 21
MaeIII GTNAC 1 cut(s) 52
MboII GAAGA 1 cut(s) 254
MluCI AATT 3 cut(s) 36, 196, 260
MnlI CCTC 7 cut(s) 84, 94, 201, 204, 238, 264, 282
MseI TTAA 2 cut(s) 123, 264
MslI CAYNNNNRTG 1 cut(s) 59
MspR9I CCNGG 1 cut(s) 142
MvaI CCWGG 1 cut(s) 142
NdeI CATATG 1 cut(s) 151
NlaIII CATG 3 cut(s) 85, 194, 297
NmuCI GTSAC 1 cut(s) 52
OliI CACNNNNGTG 1 cut(s) 59
PaeR7I CTCGAG 1 cut(s) 57
PfeI GAWTC 1 cut(s) 109
PkrI GCNGC 1 cut(s) 285
Psp6I CCWGG 1 cut(s) 140
PspGI CCWGG 1 cut(s) 140
PspXI VCTCGAGB 1 cut(s) 57
RsaI GTAC 1 cut(s) 236
RsaNI GTAC 1 cut(s) 235
RseI CAYNNNNRTG 1 cut(s) 59
SaqAI TTAA 2 cut(s) 123, 264
SatI GCNGC 1 cut(s) 284
ScrFI CCNGG 1 cut(s) 142
SetI ASST 4 cut(s) 24, 33, 189, 276
Sfr274I CTCGAG 1 cut(s) 57
SlaI CTCGAG 1 cut(s) 57
SmiMI CAYNNNNRTG 1 cut(s) 59
SmlI CTYRAG 1 cut(s) 57
SmoI CTYRAG 1 cut(s) 57
Sse9I AATT 3 cut(s) 36, 196, 260
SsiI CCGC 1 cut(s) 229
StyD4I CCNGG 1 cut(s) 140
TaiI ACGT 1 cut(s) 24
TaqI TCGA 2 cut(s) 27, 58
TasI AATT 3 cut(s) 36, 196, 260
TatI WGTACW 1 cut(s) 234
TfiI GAWTC 1 cut(s) 109
Tru1I TTAA 2 cut(s) 123, 264
Tru9I TTAA 2 cut(s) 123, 264
TscAI CASTG 1 cut(s) 93
TseFI GTSAC 1 cut(s) 52
TseI GCWGC 1 cut(s) 283
Tsp45I GTSAC 1 cut(s) 52
TspDTI ATGAA 1 cut(s) 179
TspRI CASTG 1 cut(s) 93
XapI RAATTY 1 cut(s) 260
XhoI CTCGAG 1 cut(s) 57
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.