Rroxscaffold_4G00323070

Protein of unknown function (DUF667)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0014497
Physical Location & Seq
Forward (+)
53670035 .. 53673394
3360 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00323070.1

Sequence Viewer

Length: 774 bp
ATGTTCAAGAACACATTCCAATCTGGGTTTCTTTCCATTCTATACAGCCTTGGGAGCAAACCTTTGCAGATATGGGATAAAGAAGCAGTTGTCAATGGCCATATAAAGAGACCACAGGATGAAGACATACAATCCAATGTCCTTGAAATTGTTGGATCGAATATCCAGTCCACATACATTACTTGCCCTGCAGATCCATCAGCAACACTGAGTATAAAACTGCCGTTCCTTGTTATGATTGTGAAGAATCTGAAGAAATATTTCACATTTGAGATTCAAGTTCTGGATGATAAGAATGTCAGGCGACGTTTTCGAGCTTCCAATTTTCAAGCTGTCACTCGAGTGAAGCCGTATATCTGCACCATGCCACTGAGGATGGATGAGGGCTGGAATCAAATCCAGTTTAATCTGGCTGATTTTACCAGGAGAGCATATGGAACAAACTATGTGGAGACATTGCGAGTTCAGGTTCATGCAAATTGTCGCCTGAGGAGGATATATTTCTCTGACCGCTTGTACTCTGAAGAGGAACTTCCTCCTGAATTTAAACTTTACCTTCCAATGCAGCAGGGTGATCTTGGGGACAATTCTAGTTCTGCACTCAAAAGTCCTAAGAACTTTAATATTGATTCTGTTGTTGGTAAGATTTGGAAGCGAGTGGAGACCAAAACAAAGCAAATTCTGAATTCTCCTGGTGCTAGCAAGAAGTCTACATGTAAAACTGTTGCTGCAGAGGTGCCTAAATCTGGTTCTAAAGCCAGGAGCTCCGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF667

Protein Analysis

257

Amino Acids

29.35

Weight (kDa)

9.59

Isoelectric Point (pI)

53.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CFA20_dom PF05018 1 - 185 9.3e-90 CFA20 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014497)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12300
fragaria_vesca FvH4_7g03830
malus_domestica MD02G1276800.v1.1 MD07G1044300.v1.1
prunus_persica Prupe.2G043300_v2.0.a1 Prupe.2G043300_v2.0.a1
pyrus_communis pycom02g23710
rosa_chinensis RchiOBHm_Chr1g0327201
rosa_laevigata RLG00000030035
rosa_multiflora Rmu_sc0000972.1_g000014
rosa_roxburghii Rroxscaffold_4G00323070 Rroxscaffold_4G00323150
rosa_rugosa Rorug01G0064500
rosa_samantha Rh1AG081600 Rh1BG064800 Rh1DG086100
rosa_wichuraiana Rw1G006440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 736
AccI GTMKAC 1 cut(s) 710
AciI CCGC 1 cut(s) 511
AclWI GGATC 2 cut(s) 163, 188
AcoI YGGCCR 1 cut(s) 97
AcsI RAATTY 3 cut(s) 542, 678, 685
AcuI CTGAAG 2 cut(s) 272, 543
AfaI GTAC 1 cut(s) 518
AfiI CCNNNNNNNGG 1 cut(s) 746
AflIII ACRYGT 1 cut(s) 713
AgsI TTSAA 4 cut(s) 7, 146, 278, 329
AjnI CCWGG 3 cut(s) 422, 691, 758
AjuI GAANNNNNNNTTGG 2 cut(s) 12, 44
AleI CACNNNNGTG 1 cut(s) 341
AluBI AGCT 3 cut(s) 317, 332, 765
AluI AGCT 3 cut(s) 317, 332, 765
Alw21I GWGCWC 1 cut(s) 767
Alw26I GTCTC 3 cut(s) 103, 446, 656
AlwI GGATC 2 cut(s) 163, 188
Ama87I CYCGRG 1 cut(s) 339
AoxI GGCC 1 cut(s) 97
ApeKI GCWGC 2 cut(s) 565, 728
ApoI RAATTY 3 cut(s) 542, 678, 685
ArsI GACNNNNNNTTYG 2 cut(s) 152, 184
Asp700I GAANNNNTTC 2 cut(s) 14, 260
AsuHPI GGTGA 1 cut(s) 584
AsuNHI GCTAGC 1 cut(s) 698
AvaI CYCGRG 1 cut(s) 339
AxyI CCTNAGG 1 cut(s) 488
BalI TGGCCA 1 cut(s) 99
BanI GGYRCC 1 cut(s) 736
BanII GRGCYC 1 cut(s) 767
BbsI GAAGAC 1 cut(s) 129
Bbv12I GWGCWC 1 cut(s) 767
BbvI GCAGC 2 cut(s) 577, 715
BccI CCATC 2 cut(s) 205, 370
BceAI ACGGC 2 cut(s) 208, 334
BciT130I CCWGG 3 cut(s) 424, 693, 760
BcoDI GTCTC 3 cut(s) 103, 446, 656
BfaI CTAG 2 cut(s) 591, 699
BfmI CTRYAG 2 cut(s) 189, 729
BisI GCNGC 2 cut(s) 566, 729
BlsI GCNGC 2 cut(s) 567, 730
Bme1390I CCNGG 3 cut(s) 424, 693, 760
BmeT110I CYCGRG 1 cut(s) 339
BmiI GGNNCC 1 cut(s) 738
BmrFI CCNGG 3 cut(s) 424, 693, 760
BmtI GCTAGC 1 cut(s) 702
BpiI GAAGAC 1 cut(s) 129
BsaI GGTCTC 2 cut(s) 103, 656
BsaJI CCNNGG 1 cut(s) 49
Bsc4I CCNNNNNNNGG 1 cut(s) 746
Bse1I ACTGG 2 cut(s) 166, 400
Bse21I CCTNAGG 1 cut(s) 488
Bse3DI GCAATG 1 cut(s) 455
BseBI CCWGG 3 cut(s) 424, 693, 760
BseDI CCNNGG 1 cut(s) 49
BseGI GGATG 4 cut(s) 124, 292, 381, 385
BseLI CCNNNNNNNGG 1 cut(s) 746
BseMI GCAATG 1 cut(s) 455
BseMII CTCAG 3 cut(s) 200, 362, 479
BseNI ACTGG 2 cut(s) 166, 400
BseRI GAGGAG 1 cut(s) 505
BseXI GCAGC 2 cut(s) 577, 715
BsgI GTGCAG 2 cut(s) 343, 582
BshFI GGCC 1 cut(s) 99
BshNI GGYRCC 1 cut(s) 736
BsiHKAI GWGCWC 1 cut(s) 767
BsiHKCI CYCGRG 1 cut(s) 339
BslFI GGGAC 1 cut(s) 596
BslI CCNNNNNNNGG 1 cut(s) 746
BsmAI GTCTC 3 cut(s) 103, 446, 656
BsmFI GGGAC 1 cut(s) 596
BsnI GGCC 1 cut(s) 99
Bso31I GGTCTC 2 cut(s) 103, 656
BsoBI CYCGRG 1 cut(s) 339
Bsp1286I GDGCHC 1 cut(s) 767
Bsp143I GATC 3 cut(s) 155, 193, 574
BspACI CCGC 1 cut(s) 511
BspANI GGCC 1 cut(s) 99
BspCNI CTCAG 3 cut(s) 201, 363, 480
BspLI GGNNCC 1 cut(s) 738
BspMAI CTGCAG 2 cut(s) 193, 733
BspOI GCTAGC 1 cut(s) 702
BspPI GGATC 2 cut(s) 163, 188
BspT107I GGYRCC 1 cut(s) 736
BspTNI GGTCTC 2 cut(s) 103, 656
BsrDI GCAATG 1 cut(s) 455
BsrI ACTGG 2 cut(s) 166, 400
BssECI CCNNGG 1 cut(s) 49
BssMI GATC 3 cut(s) 155, 193, 574
BssT1I CCWWGG 1 cut(s) 49
Bst2UI CCWGG 3 cut(s) 424, 693, 760
Bst4CI ACNGT 1 cut(s) 724
Bst6I CTCTTC 1 cut(s) 519
BstC8I GCNNGC 1 cut(s) 700
BstDEI CTNAG 4 cut(s) 209, 371, 488, 612
BstF5I GGATG 4 cut(s) 124, 292, 381, 385
BstKTI GATC 3 cut(s) 158, 196, 577
BstMAI GTCTC 3 cut(s) 103, 446, 656
BstMBI GATC 3 cut(s) 155, 193, 574
BstMWI GCNNNNNNNGC 1 cut(s) 54
BstNI CCWGG 3 cut(s) 424, 693, 760
BstNSI RCATGY 1 cut(s) 717
BstSCI CCNGG 3 cut(s) 422, 691, 758
BstSFI CTRYAG 2 cut(s) 189, 729
BstV1I GCAGC 2 cut(s) 577, 715
BstV2I GAAGAC 1 cut(s) 129
BstX2I RGATCY 1 cut(s) 193
BstYI RGATCY 1 cut(s) 193
Bsu36I CCTNAGG 1 cut(s) 488
BsuRI GGCC 1 cut(s) 99
BtsCI GGATG 4 cut(s) 124, 292, 381, 385
BtsIMutI CAGTG 2 cut(s) 206, 368
Cac8I GCNNGC 1 cut(s) 700
Csp6I GTAC 1 cut(s) 517
CspCI CAANNNNNGTGG 2 cut(s) 429, 464
CviAII CATG 3 cut(s) 364, 473, 714
CviJI RGCY 9 cut(s) 48, 99, 317, 332, 349, 387, 413, 758, 765
CviKI_1 RGCY 9 cut(s) 48, 99, 317, 332, 349, 387, 413, 758, 765
CviQI GTAC 1 cut(s) 517
DdeI CTNAG 4 cut(s) 209, 371, 488, 612
DpnI GATC 3 cut(s) 157, 195, 576
DpnII GATC 3 cut(s) 155, 193, 574
DraI TTTAAA 1 cut(s) 547
EaeI YGGCCR 1 cut(s) 97
Eam1104I CTCTTC 1 cut(s) 519
EarI CTCTTC 1 cut(s) 519
Ecl136II GAGCTC 1 cut(s) 765
Eco130I CCWWGG 1 cut(s) 49
Eco24I GRGCYC 1 cut(s) 767
Eco31I GGTCTC 2 cut(s) 103, 656
Eco53kI GAGCTC 1 cut(s) 765
Eco57I CTGAAG 2 cut(s) 272, 543
Eco81I CCTNAGG 1 cut(s) 488
Eco88I CYCGRG 1 cut(s) 339
EcoICRI GAGCTC 1 cut(s) 765
EcoRI GAATTC 1 cut(s) 685
EcoRII CCWGG 3 cut(s) 422, 691, 758
EcoT14I CCWWGG 1 cut(s) 49
EcoT38I GRGCYC 1 cut(s) 767
ErhI CCWWGG 1 cut(s) 49
FaeI CATG 3 cut(s) 367, 476, 717
FalI AAGNNNNNCTT 2 cut(s) 516, 548
FaqI GGGAC 1 cut(s) 596
FatI CATG 3 cut(s) 363, 472, 713
FauNDI CATATG 1 cut(s) 433
FblI GTMKAC 1 cut(s) 710
Fnu4HI GCNGC 2 cut(s) 566, 729
FokI GGATG 4 cut(s) 131, 299, 388, 392
FriOI GRGCYC 1 cut(s) 767
Fsp4HI GCNGC 2 cut(s) 566, 729
FspBI CTAG 2 cut(s) 591, 699
GluI GCNGC 2 cut(s) 566, 729
HaeIII GGCC 1 cut(s) 99
Hin1II CATG 3 cut(s) 367, 476, 717
HinfI GANTC 4 cut(s) 247, 274, 391, 629
HphI GGTGA 1 cut(s) 584
Hpy166II GTNNAC 2 cut(s) 171, 711
Hpy188I TCNGA 5 cut(s) 252, 508, 523, 684, 769
Hpy188III TCNNGA 3 cut(s) 7, 284, 539
Hpy8I GTNNAC 2 cut(s) 171, 711
Hpy99I CGWCG 1 cut(s) 309
HpyAV CCTTC 1 cut(s) 566
HpyCH4III ACNGT 1 cut(s) 724
HpyCH4IV ACGT 1 cut(s) 307
HpyCH4V TGCA 7 cut(s) 67, 191, 360, 476, 565, 599, 731
HpyF10VI GCNNNNNNNGC 1 cut(s) 54
HpyF3I CTNAG 4 cut(s) 209, 371, 488, 612
HpySE526I ACGT 1 cut(s) 307
Hsp92II CATG 3 cut(s) 367, 476, 717
Kzo9I GATC 3 cut(s) 155, 193, 574
LmnI GCTCC 3 cut(s) 54, 762, 770
Lsp1109I GCAGC 2 cut(s) 577, 715
MaeI CTAG 2 cut(s) 591, 699
MaeII ACGT 1 cut(s) 307
MaeIII GTNAC 1 cut(s) 334
MalI GATC 3 cut(s) 157, 195, 576
MboI GATC 3 cut(s) 155, 193, 574
MboII GAAGA 4 cut(s) 134, 256, 265, 536
MflI RGATCY 1 cut(s) 193
MhlI GDGCHC 1 cut(s) 767
MlsI TGGCCA 1 cut(s) 99
MluCI AATT 7 cut(s) 147, 322, 478, 542, 586, 678, 685
MluNI TGGCCA 1 cut(s) 99
MmeI TCCRAC 1 cut(s) 133
MnlI CCTC 7 cut(s) 366, 376, 483, 486, 520, 546, 727
Mox20I TGGCCA 1 cut(s) 99
MroXI GAANNNNTTC 2 cut(s) 14, 260
MscI TGGCCA 1 cut(s) 99
MseI TTAA 3 cut(s) 405, 546, 621
MslI CAYNNNNRTG 1 cut(s) 341
Msp20I TGGCCA 1 cut(s) 99
MspR9I CCNGG 3 cut(s) 424, 693, 760
MvaI CCWGG 3 cut(s) 424, 693, 760
MwoI GCNNNNNNNGC 1 cut(s) 54
NdeI CATATG 1 cut(s) 433
NdeII GATC 3 cut(s) 155, 193, 574
NheI GCTAGC 1 cut(s) 698
NlaIII CATG 3 cut(s) 367, 476, 717
NlaIV GGNNCC 1 cut(s) 738
NmuCI GTSAC 1 cut(s) 334
NspI RCATGY 1 cut(s) 717
OliI CACNNNNGTG 1 cut(s) 341
PaeR7I CTCGAG 1 cut(s) 339
PciI ACATGT 1 cut(s) 713
PdmI GAANNNNTTC 2 cut(s) 14, 260
PfeI GAWTC 4 cut(s) 247, 274, 391, 629
PkrI GCNGC 2 cut(s) 567, 730
PscI ACATGT 1 cut(s) 713
Psp124BI GAGCTC 1 cut(s) 767
Psp6I CCWGG 3 cut(s) 422, 691, 758
PspGI CCWGG 3 cut(s) 422, 691, 758
PspN4I GGNNCC 1 cut(s) 738
PspXI VCTCGAGB 1 cut(s) 339
PstI CTGCAG 2 cut(s) 193, 733
PsuI RGATCY 1 cut(s) 193
RsaI GTAC 1 cut(s) 518
RsaNI GTAC 1 cut(s) 517
RseI CAYNNNNRTG 1 cut(s) 341
SacI GAGCTC 1 cut(s) 767
SaqAI TTAA 3 cut(s) 405, 546, 621
SatI GCNGC 2 cut(s) 566, 729
Sau3AI GATC 3 cut(s) 155, 193, 574
ScrFI CCNGG 3 cut(s) 424, 693, 760
SduI GDGCHC 1 cut(s) 767
SetI ASST 8 cut(s) 64, 310, 319, 334, 471, 558, 738, 767
SfcI CTRYAG 2 cut(s) 189, 729
Sfr274I CTCGAG 1 cut(s) 339
SlaI CTCGAG 1 cut(s) 339
SmiMI CAYNNNNRTG 1 cut(s) 341
SmlI CTYRAG 1 cut(s) 339
SmoI CTYRAG 1 cut(s) 339
Sse9I AATT 7 cut(s) 147, 322, 478, 542, 586, 678, 685
SsiI CCGC 1 cut(s) 511
SspI AATATT 2 cut(s) 260, 625
SspMI CTAG 2 cut(s) 591, 699
SstI GAGCTC 1 cut(s) 767
StyD4I CCNGG 3 cut(s) 422, 691, 758
StyI CCWWGG 1 cut(s) 49
TaaI ACNGT 1 cut(s) 724
TaiI ACGT 1 cut(s) 310
TaqI TCGA 3 cut(s) 158, 313, 340
TasI AATT 7 cut(s) 147, 322, 478, 542, 586, 678, 685
TatI WGTACW 1 cut(s) 516
TfiI GAWTC 4 cut(s) 247, 274, 391, 629
Tru1I TTAA 3 cut(s) 405, 546, 621
Tru9I TTAA 3 cut(s) 405, 546, 621
TscAI CASTG 2 cut(s) 213, 375
TseFI GTSAC 1 cut(s) 334
TseI GCWGC 2 cut(s) 565, 728
Tsp45I GTSAC 1 cut(s) 334
TspDTI ATGAA 2 cut(s) 135, 461
TspRI CASTG 2 cut(s) 213, 375
XapI RAATTY 3 cut(s) 542, 678, 685
XceI RCATGY 1 cut(s) 717
XhoI CTCGAG 1 cut(s) 339
XmiI GTMKAC 1 cut(s) 710
XmnI GAANNNNTTC 2 cut(s) 14, 260
XspI CTAG 2 cut(s) 591, 699
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.