RLG00000030035

Protein of unknown function (DUF667)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0014497
Physical Location & Seq
Forward (+)
53669814 .. 53670995
1182 bp
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UTR
Exon/CDS
Intron
RLM00000030035

Sequence Viewer

Length: 516 bp
ATGTGTTTGATTTCATGGCAAGCAGTTGTCAATGGCCATATAAAGAGACCACAGGATGAAGACATACAATCCAATGTCCTTGAAATTGTTGGATCGAATATCCAGTCCACATACATTACTTGCCCTGCAGATCCATCAGCAACACTGAGTATAAAACTGCCGTTCCTTGTTATGATTGTGAAGAATCTGAAGAAATATTTCACATTTGAGATTCAAGTTCTGGATGATAAGAATGTCAGGCGACGTTTTCGAGCTTCCAATTTTCAAGCTGTCACTCGAGTGAAGCCATATATCTGCACCATGCCACTGAGGATGGATGAGGGCTGGAATCAAATCCAGTTTAATCTGGCTGATTTTACCAGGAGAGCCTATGGAACAAACTATGTGGAGACATTGCGAGTTCAGGTTCATGCAAATTGTCGCCTGAGGAGGATATATTTCTCTGACCGCTTGTACTCTGAAGAGGAACTTCCTCCTGAATTTAAACTATACCTTCCAATGCAGCAGAGAACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF667

Protein Analysis

172

Amino Acids

20.17

Weight (kDa)

9.25

Isoelectric Point (pI)

54.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CFA20_dom PF05018 6 - 164 4.8e-78 CFA20 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014497)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12300
fragaria_vesca FvH4_7g03830
malus_domestica MD02G1276800.v1.1 MD07G1044300.v1.1
prunus_persica Prupe.2G043300_v2.0.a1 Prupe.2G043300_v2.0.a1
pyrus_communis pycom02g23710
rosa_chinensis RchiOBHm_Chr1g0327201
rosa_laevigata RLG00000030035
rosa_multiflora Rmu_sc0000972.1_g000014
rosa_roxburghii Rroxscaffold_4G00323070 Rroxscaffold_4G00323150
rosa_rugosa Rorug01G0064500
rosa_samantha Rh1AG081600 Rh1BG064800 Rh1DG086100
rosa_wichuraiana Rw1G006440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 448
AclWI GGATC 2 cut(s) 100, 125
AcoI YGGCCR 1 cut(s) 34
AcsI RAATTY 1 cut(s) 479
AcuI CTGAAG 2 cut(s) 209, 480
AfaI GTAC 1 cut(s) 455
AgsI TTSAA 3 cut(s) 83, 215, 266
AjnI CCWGG 1 cut(s) 359
AleI CACNNNNGTG 1 cut(s) 278
AluBI AGCT 2 cut(s) 254, 269
AluI AGCT 2 cut(s) 254, 269
Alw26I GTCTC 2 cut(s) 40, 383
AlwI GGATC 2 cut(s) 100, 125
Ama87I CYCGRG 1 cut(s) 276
AoxI GGCC 1 cut(s) 34
ApeKI GCWGC 1 cut(s) 502
ApoI RAATTY 1 cut(s) 479
ArsI GACNNNNNNTTYG 2 cut(s) 89, 121
Asp700I GAANNNNTTC 1 cut(s) 197
AvaI CYCGRG 1 cut(s) 276
AxyI CCTNAGG 1 cut(s) 425
BalI TGGCCA 1 cut(s) 36
BbsI GAAGAC 1 cut(s) 66
BccI CCATC 2 cut(s) 142, 307
BceAI ACGGC 1 cut(s) 145
BciT130I CCWGG 1 cut(s) 361
BcoDI GTCTC 2 cut(s) 40, 383
BfmI CTRYAG 1 cut(s) 126
BisI GCNGC 1 cut(s) 503
BlsI GCNGC 1 cut(s) 504
Bme1390I CCNGG 1 cut(s) 361
BmeT110I CYCGRG 1 cut(s) 276
BmrFI CCNGG 1 cut(s) 361
BpiI GAAGAC 1 cut(s) 66
BsaI GGTCTC 1 cut(s) 40
Bse1I ACTGG 2 cut(s) 103, 337
Bse21I CCTNAGG 1 cut(s) 425
Bse3DI GCAATG 1 cut(s) 392
BseBI CCWGG 1 cut(s) 361
BseGI GGATG 4 cut(s) 61, 229, 318, 322
BseMI GCAATG 1 cut(s) 392
BseMII CTCAG 3 cut(s) 137, 299, 416
BseNI ACTGG 2 cut(s) 103, 337
BseRI GAGGAG 1 cut(s) 442
BsgI GTGCAG 1 cut(s) 280
BshFI GGCC 1 cut(s) 36
BsiHKCI CYCGRG 1 cut(s) 276
BsmAI GTCTC 2 cut(s) 40, 383
BsnI GGCC 1 cut(s) 36
Bso31I GGTCTC 1 cut(s) 40
BsoBI CYCGRG 1 cut(s) 276
Bsp143I GATC 2 cut(s) 92, 130
BspACI CCGC 1 cut(s) 448
BspANI GGCC 1 cut(s) 36
BspCNI CTCAG 3 cut(s) 138, 300, 417
BspMAI CTGCAG 1 cut(s) 130
BspPI GGATC 2 cut(s) 100, 125
BspTNI GGTCTC 1 cut(s) 40
BsrDI GCAATG 1 cut(s) 392
BsrI ACTGG 2 cut(s) 103, 337
BssMI GATC 2 cut(s) 92, 130
Bst2UI CCWGG 1 cut(s) 361
Bst6I CTCTTC 1 cut(s) 456
BstC8I GCNNGC 1 cut(s) 21
BstDEI CTNAG 3 cut(s) 146, 308, 425
BstF5I GGATG 4 cut(s) 61, 229, 318, 322
BstKTI GATC 2 cut(s) 95, 133
BstMAI GTCTC 2 cut(s) 40, 383
BstMBI GATC 2 cut(s) 92, 130
BstNI CCWGG 1 cut(s) 361
BstSCI CCNGG 1 cut(s) 359
BstSFI CTRYAG 1 cut(s) 126
BstV2I GAAGAC 1 cut(s) 66
BstX2I RGATCY 1 cut(s) 130
BstYI RGATCY 1 cut(s) 130
Bsu36I CCTNAGG 1 cut(s) 425
BsuRI GGCC 1 cut(s) 36
BtsCI GGATG 4 cut(s) 61, 229, 318, 322
BtsIMutI CAGTG 2 cut(s) 143, 305
Cac8I GCNNGC 1 cut(s) 21
Csp6I GTAC 1 cut(s) 454
CspCI CAANNNNNGTGG 2 cut(s) 366, 401
CviAII CATG 3 cut(s) 15, 301, 410
CviJI RGCY 7 cut(s) 36, 254, 269, 286, 324, 350, 368
CviKI_1 RGCY 7 cut(s) 36, 254, 269, 286, 324, 350, 368
CviQI GTAC 1 cut(s) 454
DdeI CTNAG 3 cut(s) 146, 308, 425
DpnI GATC 2 cut(s) 94, 132
DpnII GATC 2 cut(s) 92, 130
DraI TTTAAA 1 cut(s) 484
EaeI YGGCCR 1 cut(s) 34
Eam1104I CTCTTC 1 cut(s) 456
EarI CTCTTC 1 cut(s) 456
Eco31I GGTCTC 1 cut(s) 40
Eco57I CTGAAG 2 cut(s) 209, 480
Eco81I CCTNAGG 1 cut(s) 425
Eco88I CYCGRG 1 cut(s) 276
EcoRII CCWGG 1 cut(s) 359
FaeI CATG 3 cut(s) 18, 304, 413
FalI AAGNNNNNCTT 2 cut(s) 453, 485
FatI CATG 3 cut(s) 14, 300, 409
Fnu4HI GCNGC 1 cut(s) 503
FokI GGATG 4 cut(s) 68, 236, 325, 329
Fsp4HI GCNGC 1 cut(s) 503
GluI GCNGC 1 cut(s) 503
HaeIII GGCC 1 cut(s) 36
Hin1II CATG 3 cut(s) 18, 304, 413
HinfI GANTC 3 cut(s) 184, 211, 328
Hpy166II GTNNAC 1 cut(s) 108
Hpy188I TCNGA 3 cut(s) 189, 445, 460
Hpy188III TCNNGA 2 cut(s) 221, 476
Hpy8I GTNNAC 1 cut(s) 108
Hpy99I CGWCG 1 cut(s) 246
HpyAV CCTTC 1 cut(s) 503
HpyCH4IV ACGT 1 cut(s) 244
HpyCH4V TGCA 4 cut(s) 128, 297, 413, 502
HpyF3I CTNAG 3 cut(s) 146, 308, 425
HpySE526I ACGT 1 cut(s) 244
Hsp92II CATG 3 cut(s) 18, 304, 413
Kzo9I GATC 2 cut(s) 92, 130
MaeII ACGT 1 cut(s) 244
MaeIII GTNAC 1 cut(s) 271
MalI GATC 2 cut(s) 94, 132
MboI GATC 2 cut(s) 92, 130
MboII GAAGA 4 cut(s) 71, 193, 202, 473
MflI RGATCY 1 cut(s) 130
MlsI TGGCCA 1 cut(s) 36
MluCI AATT 4 cut(s) 84, 259, 415, 479
MluNI TGGCCA 1 cut(s) 36
MmeI TCCRAC 1 cut(s) 70
MnlI CCTC 6 cut(s) 303, 313, 420, 423, 457, 483
Mox20I TGGCCA 1 cut(s) 36
MroXI GAANNNNTTC 1 cut(s) 197
MscI TGGCCA 1 cut(s) 36
MseI TTAA 2 cut(s) 342, 483
MslI CAYNNNNRTG 1 cut(s) 278
Msp20I TGGCCA 1 cut(s) 36
MspR9I CCNGG 1 cut(s) 361
MvaI CCWGG 1 cut(s) 361
NdeII GATC 2 cut(s) 92, 130
NlaIII CATG 3 cut(s) 18, 304, 413
NmuCI GTSAC 1 cut(s) 271
OliI CACNNNNGTG 1 cut(s) 278
PaeR7I CTCGAG 1 cut(s) 276
PdmI GAANNNNTTC 1 cut(s) 197
PfeI GAWTC 3 cut(s) 184, 211, 328
PkrI GCNGC 1 cut(s) 504
Psp6I CCWGG 1 cut(s) 359
PspGI CCWGG 1 cut(s) 359
PspXI VCTCGAGB 1 cut(s) 276
PstI CTGCAG 1 cut(s) 130
PsuI RGATCY 1 cut(s) 130
RsaI GTAC 1 cut(s) 455
RsaNI GTAC 1 cut(s) 454
RseI CAYNNNNRTG 1 cut(s) 278
SaqAI TTAA 2 cut(s) 342, 483
SatI GCNGC 1 cut(s) 503
Sau3AI GATC 2 cut(s) 92, 130
ScrFI CCNGG 1 cut(s) 361
SetI ASST 5 cut(s) 247, 256, 271, 408, 495
SfcI CTRYAG 1 cut(s) 126
Sfr274I CTCGAG 1 cut(s) 276
SlaI CTCGAG 1 cut(s) 276
SmiMI CAYNNNNRTG 1 cut(s) 278
SmlI CTYRAG 1 cut(s) 276
SmoI CTYRAG 1 cut(s) 276
Sse9I AATT 4 cut(s) 84, 259, 415, 479
SsiI CCGC 1 cut(s) 448
SspI AATATT 1 cut(s) 197
StyD4I CCNGG 1 cut(s) 359
TaiI ACGT 1 cut(s) 247
TaqI TCGA 3 cut(s) 95, 250, 277
TasI AATT 4 cut(s) 84, 259, 415, 479
TatI WGTACW 1 cut(s) 453
TfiI GAWTC 3 cut(s) 184, 211, 328
Tru1I TTAA 2 cut(s) 342, 483
Tru9I TTAA 2 cut(s) 342, 483
TscAI CASTG 2 cut(s) 150, 312
TseFI GTSAC 1 cut(s) 271
TseI GCWGC 1 cut(s) 502
Tsp45I GTSAC 1 cut(s) 271
TspDTI ATGAA 2 cut(s) 72, 398
TspRI CASTG 2 cut(s) 150, 312
XapI RAATTY 1 cut(s) 479
XhoI CTCGAG 1 cut(s) 276
XmnI GAANNNNTTC 1 cut(s) 197
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.