Rw1G006440

Protein of unknown function (DUF667)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0014497
Physical Location & Seq
Reverse (-)
13239628 .. 13243449
3822 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G006440.1

Sequence Viewer

Length: 618 bp
ATGTTCAAGAACACATTCCAATCTGGGTTTCTGTCCGTTCTATACAGCCTTGGGAGCAAACCTTTGCAGATATGGGATAAAGAAGTTGTCAATGGCCATATAAAGAGACCACAGGATGAAGACATACAATCCAATGTCCTTGAAATTGTTGGATCGAATATCCAGTCCACATACATTACTTGCCCTGCAGATCCATCAGCAACACTGAGTATAAAACTGCCGTTCCTTGTTATGATTGTGAAGAATCTGAAGAAATATTTCACATTTGAGATTCAAGTTCTGGATGATAAGAATGTCAGGCGACGTTTTCGAGCTTCCAATTTTCAAGCTGTCACTCGAGTGAAGCCGTATATCTGCACCATGCCACTGAGGATGGATGAGGGCTGGAATCAAATCCAGTTTAATCTGGCTGATTTTACCAGGAGAGCCTATGGAACAAACTATGTGGAGACATTGCGAGTTCAGGTTCATGCAAATTGTCGCCTGAGGAGGATATATTTCTCTGACCGCTTGTACTCTGAAGAGGAACTTCCTCCTGAATTTAAACTATACCTTCCAATGCAGCAGGTAATCGATGGAGACCAAAACAAAGCAAATTCTGAGTTCTCCAGGTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF667

Protein Analysis

205

Amino Acids

24.0

Weight (kDa)

9.01

Isoelectric Point (pI)

51.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CFA20_dom PF05018 1 - 184 5.5e-91 CFA20 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014497)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12300
fragaria_vesca FvH4_7g03830
malus_domestica MD02G1276800.v1.1 MD07G1044300.v1.1
prunus_persica Prupe.2G043300_v2.0.a1 Prupe.2G043300_v2.0.a1
pyrus_communis pycom02g23710
rosa_chinensis RchiOBHm_Chr1g0327201
rosa_laevigata RLG00000030035
rosa_multiflora Rmu_sc0000972.1_g000014
rosa_roxburghii Rroxscaffold_4G00323070 Rroxscaffold_4G00323150
rosa_rugosa Rorug01G0064500
rosa_samantha Rh1AG081600 Rh1BG064800 Rh1DG086100
rosa_wichuraiana Rw1G006440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 556
AciI CCGC 1 cut(s) 508
AclWI GGATC 2 cut(s) 160, 185
AcoI YGGCCR 1 cut(s) 94
AcsI RAATTY 2 cut(s) 539, 595
AcuI CTGAAG 2 cut(s) 269, 540
AfaI GTAC 1 cut(s) 515
AgsI TTSAA 4 cut(s) 7, 143, 275, 326
AjnI CCWGG 2 cut(s) 419, 608
AjuI GAANNNNNNNTTGG 2 cut(s) 12, 44
AleI CACNNNNGTG 1 cut(s) 338
AluBI AGCT 2 cut(s) 314, 329
AluI AGCT 2 cut(s) 314, 329
Alw26I GTCTC 3 cut(s) 100, 443, 573
AlwI GGATC 2 cut(s) 160, 185
Ama87I CYCGRG 1 cut(s) 336
AoxI GGCC 1 cut(s) 94
ApeKI GCWGC 1 cut(s) 562
ApoI RAATTY 2 cut(s) 539, 595
ArsI GACNNNNNNTTYG 2 cut(s) 149, 181
Asp700I GAANNNNTTC 2 cut(s) 14, 257
AvaI CYCGRG 1 cut(s) 336
AxyI CCTNAGG 1 cut(s) 485
BalI TGGCCA 1 cut(s) 96
BbsI GAAGAC 1 cut(s) 126
BbvI GCAGC 1 cut(s) 574
BccI CCATC 3 cut(s) 202, 367, 569
BceAI ACGGC 2 cut(s) 205, 331
BciT130I CCWGG 2 cut(s) 421, 610
BcoDI GTCTC 3 cut(s) 100, 443, 573
BfaI CTAG 1 cut(s) 616
BfmI CTRYAG 1 cut(s) 186
BfuAI ACCTGC 1 cut(s) 556
BisI GCNGC 1 cut(s) 563
BlsI GCNGC 1 cut(s) 564
Bme1390I CCNGG 2 cut(s) 421, 610
BmeT110I CYCGRG 1 cut(s) 336
BmrFI CCNGG 2 cut(s) 421, 610
BpiI GAAGAC 1 cut(s) 126
BpmI CTGGAG 1 cut(s) 592
Bsa29I ATCGAT 1 cut(s) 573
BsaI GGTCTC 2 cut(s) 100, 573
BsaJI CCNNGG 1 cut(s) 49
Bse1I ACTGG 2 cut(s) 163, 397
Bse21I CCTNAGG 1 cut(s) 485
Bse3DI GCAATG 1 cut(s) 452
BseBI CCWGG 2 cut(s) 421, 610
BseCI ATCGAT 1 cut(s) 573
BseDI CCNNGG 1 cut(s) 49
BseGI GGATG 4 cut(s) 121, 289, 378, 382
BseMI GCAATG 1 cut(s) 452
BseMII CTCAG 4 cut(s) 197, 359, 476, 591
BseNI ACTGG 2 cut(s) 163, 397
BseRI GAGGAG 1 cut(s) 502
BseXI GCAGC 1 cut(s) 574
BsgI GTGCAG 1 cut(s) 340
BshFI GGCC 1 cut(s) 96
BshVI ATCGAT 1 cut(s) 573
BsiHKCI CYCGRG 1 cut(s) 336
BsmAI GTCTC 3 cut(s) 100, 443, 573
BsnI GGCC 1 cut(s) 96
Bso31I GGTCTC 2 cut(s) 100, 573
BsoBI CYCGRG 1 cut(s) 336
Bsp143I GATC 2 cut(s) 152, 190
BspACI CCGC 1 cut(s) 508
BspANI GGCC 1 cut(s) 96
BspCNI CTCAG 4 cut(s) 198, 360, 477, 592
BspDI ATCGAT 1 cut(s) 573
BspMAI CTGCAG 1 cut(s) 190
BspMI ACCTGC 1 cut(s) 556
BspPI GGATC 2 cut(s) 160, 185
BspTNI GGTCTC 2 cut(s) 100, 573
BsrDI GCAATG 1 cut(s) 452
BsrI ACTGG 2 cut(s) 163, 397
BssECI CCNNGG 1 cut(s) 49
BssMI GATC 2 cut(s) 152, 190
BssT1I CCWWGG 1 cut(s) 49
Bst2UI CCWGG 2 cut(s) 421, 610
Bst6I CTCTTC 1 cut(s) 516
BstDEI CTNAG 4 cut(s) 206, 368, 485, 600
BstF5I GGATG 4 cut(s) 121, 289, 378, 382
BstKTI GATC 2 cut(s) 155, 193
BstMAI GTCTC 3 cut(s) 100, 443, 573
BstMBI GATC 2 cut(s) 152, 190
BstMWI GCNNNNNNNGC 1 cut(s) 54
BstNI CCWGG 2 cut(s) 421, 610
BstSCI CCNGG 2 cut(s) 419, 608
BstSFI CTRYAG 1 cut(s) 186
BstV1I GCAGC 1 cut(s) 574
BstV2I GAAGAC 1 cut(s) 126
BstX2I RGATCY 1 cut(s) 190
BstYI RGATCY 1 cut(s) 190
Bsu15I ATCGAT 1 cut(s) 573
Bsu36I CCTNAGG 1 cut(s) 485
BsuRI GGCC 1 cut(s) 96
BsuTUI ATCGAT 1 cut(s) 573
BtsCI GGATG 4 cut(s) 121, 289, 378, 382
BtsIMutI CAGTG 2 cut(s) 203, 365
BveI ACCTGC 1 cut(s) 556
ClaI ATCGAT 1 cut(s) 573
Csp6I GTAC 1 cut(s) 514
CspCI CAANNNNNGTGG 2 cut(s) 426, 461
CviAII CATG 2 cut(s) 361, 470
CviJI RGCY 8 cut(s) 48, 96, 314, 329, 346, 384, 410, 428
CviKI_1 RGCY 8 cut(s) 48, 96, 314, 329, 346, 384, 410, 428
CviQI GTAC 1 cut(s) 514
DdeI CTNAG 4 cut(s) 206, 368, 485, 600
DpnI GATC 2 cut(s) 154, 192
DpnII GATC 2 cut(s) 152, 190
DraI TTTAAA 1 cut(s) 544
EaeI YGGCCR 1 cut(s) 94
Eam1104I CTCTTC 1 cut(s) 516
EarI CTCTTC 1 cut(s) 516
Eco130I CCWWGG 1 cut(s) 49
Eco31I GGTCTC 2 cut(s) 100, 573
Eco57I CTGAAG 2 cut(s) 269, 540
Eco81I CCTNAGG 1 cut(s) 485
Eco88I CYCGRG 1 cut(s) 336
EcoRII CCWGG 2 cut(s) 419, 608
EcoT14I CCWWGG 1 cut(s) 49
ErhI CCWWGG 1 cut(s) 49
FaeI CATG 2 cut(s) 364, 473
FalI AAGNNNNNCTT 2 cut(s) 513, 545
FatI CATG 2 cut(s) 360, 469
Fnu4HI GCNGC 1 cut(s) 563
FokI GGATG 4 cut(s) 128, 296, 385, 389
Fsp4HI GCNGC 1 cut(s) 563
FspBI CTAG 1 cut(s) 616
GluI GCNGC 1 cut(s) 563
GsuI CTGGAG 1 cut(s) 592
HaeIII GGCC 1 cut(s) 96
Hin1II CATG 2 cut(s) 364, 473
HinfI GANTC 3 cut(s) 244, 271, 388
Hpy166II GTNNAC 1 cut(s) 168
Hpy188I TCNGA 4 cut(s) 249, 505, 520, 601
Hpy188III TCNNGA 3 cut(s) 7, 281, 536
Hpy8I GTNNAC 1 cut(s) 168
Hpy99I CGWCG 1 cut(s) 306
HpyAV CCTTC 1 cut(s) 563
HpyCH4IV ACGT 1 cut(s) 304
HpyCH4V TGCA 5 cut(s) 67, 188, 357, 473, 562
HpyF10VI GCNNNNNNNGC 1 cut(s) 54
HpyF3I CTNAG 4 cut(s) 206, 368, 485, 600
HpySE526I ACGT 1 cut(s) 304
Hsp92II CATG 2 cut(s) 364, 473
Kzo9I GATC 2 cut(s) 152, 190
LmnI GCTCC 1 cut(s) 54
Lsp1109I GCAGC 1 cut(s) 574
MaeI CTAG 1 cut(s) 616
MaeII ACGT 1 cut(s) 304
MaeIII GTNAC 1 cut(s) 331
MalI GATC 2 cut(s) 154, 192
MboI GATC 2 cut(s) 152, 190
MboII GAAGA 4 cut(s) 131, 253, 262, 533
MflI RGATCY 1 cut(s) 190
MlsI TGGCCA 1 cut(s) 96
MluCI AATT 5 cut(s) 144, 319, 475, 539, 595
MluNI TGGCCA 1 cut(s) 96
MmeI TCCRAC 1 cut(s) 130
MnlI CCTC 6 cut(s) 363, 373, 480, 483, 517, 543
Mox20I TGGCCA 1 cut(s) 96
MroXI GAANNNNTTC 2 cut(s) 14, 257
MscI TGGCCA 1 cut(s) 96
MseI TTAA 2 cut(s) 402, 543
MslI CAYNNNNRTG 1 cut(s) 338
Msp20I TGGCCA 1 cut(s) 96
MspR9I CCNGG 2 cut(s) 421, 610
MvaI CCWGG 2 cut(s) 421, 610
MwoI GCNNNNNNNGC 1 cut(s) 54
NdeII GATC 2 cut(s) 152, 190
NlaIII CATG 2 cut(s) 364, 473
NmuCI GTSAC 1 cut(s) 331
OliI CACNNNNGTG 1 cut(s) 338
PaeR7I CTCGAG 1 cut(s) 336
PdmI GAANNNNTTC 2 cut(s) 14, 257
PfeI GAWTC 3 cut(s) 244, 271, 388
PkrI GCNGC 1 cut(s) 564
Psp6I CCWGG 2 cut(s) 419, 608
PspGI CCWGG 2 cut(s) 419, 608
PspXI VCTCGAGB 1 cut(s) 336
PstI CTGCAG 1 cut(s) 190
PsuI RGATCY 1 cut(s) 190
RsaI GTAC 1 cut(s) 515
RsaNI GTAC 1 cut(s) 514
RseI CAYNNNNRTG 1 cut(s) 338
SaqAI TTAA 2 cut(s) 402, 543
SatI GCNGC 1 cut(s) 563
Sau3AI GATC 2 cut(s) 152, 190
ScrFI CCNGG 2 cut(s) 421, 610
SetI ASST 8 cut(s) 64, 307, 316, 331, 468, 555, 570, 614
SfcI CTRYAG 1 cut(s) 186
Sfr274I CTCGAG 1 cut(s) 336
SlaI CTCGAG 1 cut(s) 336
SmiMI CAYNNNNRTG 1 cut(s) 338
SmlI CTYRAG 1 cut(s) 336
SmoI CTYRAG 1 cut(s) 336
Sse9I AATT 5 cut(s) 144, 319, 475, 539, 595
SsiI CCGC 1 cut(s) 508
SspI AATATT 1 cut(s) 257
SspMI CTAG 1 cut(s) 616
StyD4I CCNGG 2 cut(s) 419, 608
StyI CCWWGG 1 cut(s) 49
TaiI ACGT 1 cut(s) 307
TaqI TCGA 4 cut(s) 155, 310, 337, 573
TasI AATT 5 cut(s) 144, 319, 475, 539, 595
TatI WGTACW 1 cut(s) 513
TfiI GAWTC 3 cut(s) 244, 271, 388
Tru1I TTAA 2 cut(s) 402, 543
Tru9I TTAA 2 cut(s) 402, 543
TscAI CASTG 2 cut(s) 210, 372
TseFI GTSAC 1 cut(s) 331
TseI GCWGC 1 cut(s) 562
Tsp45I GTSAC 1 cut(s) 331
TspDTI ATGAA 2 cut(s) 132, 458
TspGWI ACGGA 1 cut(s) 25
TspRI CASTG 2 cut(s) 210, 372
XapI RAATTY 2 cut(s) 539, 595
XhoI CTCGAG 1 cut(s) 336
XmnI GAANNNNTTC 2 cut(s) 14, 257
XspI CTAG 1 cut(s) 616
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.