MD02G1276800.v1.1

Protein of unknown function (DUF667)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0014497
Physical Location & Seq
Forward (+)
33258675 .. 33260736
2062 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1276800.v1.1.491

Sequence Viewer

Length: 402 bp
ATGTTCAAGAATACGTTTCAATCCGGATTTCTATCCATTCTATACAGCCTTGGGAGTAAACCTTTGCAGATTTGGGATAAAGAAGTTGTTGATGGGCATATAAAAAGACCACAGGATGAAGACATACAATCCAATGTTCTTGAAATAGTTGGGTCGAACATCCAGTCAACATACATTACCTGCCCGGCAGACCCATCAGCTACACTGAGTATAAAGCTTCCATTCCTGGTTATGATTGTGAAGAATCTGAAGAAATATTTCACATTTGAGATTCAAATTCTGGATGACAAGAATGTCAGGCGACGTTTTCGAGCTTCCAATTTTCAAGTGTGGAATATTCTGGTACACGTAATGCTTCTTATGTCTGTCTCAAAGATATCTATATTTCACAAACAAGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF667

Protein Analysis

134

Amino Acids

15.39

Weight (kDa)

9.48

Isoelectric Point (pI)

60.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CFA20_dom PF05018 1 - 121 2.4e-49 CFA20 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014497)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12300
fragaria_vesca FvH4_7g03830
malus_domestica MD02G1276800.v1.1 MD07G1044300.v1.1
prunus_persica Prupe.2G043300_v2.0.a1 Prupe.2G043300_v2.0.a1
pyrus_communis pycom02g23710
rosa_chinensis RchiOBHm_Chr1g0327201
rosa_laevigata RLG00000030035
rosa_multiflora Rmu_sc0000972.1_g000014
rosa_roxburghii Rroxscaffold_4G00323070 Rroxscaffold_4G00323150
rosa_rugosa Rorug01G0064500
rosa_samantha Rh1AG081600 Rh1BG064800 Rh1DG086100
rosa_wichuraiana Rw1G006440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 293
Acc36I ACCTGC 1 cut(s) 188
AccIII TCCGGA 1 cut(s) 23
AcsI RAATTY 1 cut(s) 276
AcuI CTGAAG 1 cut(s) 269
AfaI GTAC 1 cut(s) 345
AflIII ACRYGT 1 cut(s) 346
AgsI TTSAA 5 cut(s) 7, 20, 143, 275, 326
AjnI CCWGG 1 cut(s) 225
AluBI AGCT 3 cut(s) 200, 217, 314
AluI AGCT 3 cut(s) 200, 217, 314
Alw26I GTCTC 1 cut(s) 373
Aor13HI TCCGGA 1 cut(s) 23
ApoI RAATTY 1 cut(s) 276
ArsI GACNNNNNNTTYG 2 cut(s) 149, 181
Asp700I GAANNNNTTC 1 cut(s) 257
AsuC2I CCSGG 1 cut(s) 185
BbsI GAAGAC 1 cut(s) 126
BccI CCATC 2 cut(s) 86, 202
BciT130I CCWGG 1 cut(s) 227
BcnI CCSGG 1 cut(s) 185
BcoDI GTCTC 1 cut(s) 373
BfuAI ACCTGC 1 cut(s) 188
Bme1390I CCNGG 2 cut(s) 185, 227
BmrFI CCNGG 2 cut(s) 185, 227
BpiI GAAGAC 1 cut(s) 126
BpuMI CCSGG 1 cut(s) 185
BsaAI YACGTR 1 cut(s) 349
BsaBI GATNNNNATC 1 cut(s) 31
BsaJI CCNNGG 1 cut(s) 49
BsaWI WCCGGW 1 cut(s) 23
Bse1I ACTGG 1 cut(s) 163
Bse8I GATNNNNATC 1 cut(s) 31
BseAI TCCGGA 1 cut(s) 23
BseBI CCWGG 1 cut(s) 227
BseDI CCNNGG 1 cut(s) 49
BseGI GGATG 3 cut(s) 121, 159, 289
BseJI GATNNNNATC 1 cut(s) 31
BseMII CTCAG 1 cut(s) 197
BseNI ACTGG 1 cut(s) 163
BsiSI CCGG 2 cut(s) 24, 185
BsmAI GTCTC 1 cut(s) 373
Bsp13I TCCGGA 1 cut(s) 23
BspCNI CTCAG 1 cut(s) 198
BspEI TCCGGA 1 cut(s) 23
BspMI ACCTGC 1 cut(s) 188
BsrI ACTGG 1 cut(s) 163
BssECI CCNNGG 1 cut(s) 49
BssT1I CCWWGG 1 cut(s) 49
Bst2UI CCWGG 1 cut(s) 227
BstBAI YACGTR 1 cut(s) 349
BstDEI CTNAG 1 cut(s) 206
BstF5I GGATG 3 cut(s) 121, 159, 289
BstMAI GTCTC 1 cut(s) 373
BstNI CCWGG 1 cut(s) 227
BstSCI CCNGG 2 cut(s) 183, 225
BstV2I GAAGAC 1 cut(s) 126
BtsCI GGATG 3 cut(s) 121, 159, 289
BtsIMutI CAGTG 1 cut(s) 203
BveI ACCTGC 1 cut(s) 188
Csp6I GTAC 1 cut(s) 344
CviJI RGCY 4 cut(s) 48, 200, 217, 314
CviKI_1 RGCY 4 cut(s) 48, 200, 217, 314
CviQI GTAC 1 cut(s) 344
DdeI CTNAG 1 cut(s) 206
DrdI GACNNNNNNGTC 1 cut(s) 293
DseDI GACNNNNNNGTC 1 cut(s) 293
Eco130I CCWWGG 1 cut(s) 49
Eco32I GATATC 1 cut(s) 378
Eco57I CTGAAG 1 cut(s) 269
EcoRII CCWGG 1 cut(s) 225
EcoRV GATATC 1 cut(s) 378
EcoT14I CCWWGG 1 cut(s) 49
ErhI CCWWGG 1 cut(s) 49
FaiI YATR 9 cut(s) 43, 99, 101, 125, 172, 212, 233, 362, 383
FokI GGATG 3 cut(s) 128, 146, 296
HapII CCGG 2 cut(s) 24, 185
HincII GTYRAC 1 cut(s) 168
HindII GTYRAC 1 cut(s) 168
HindIII AAGCTT 1 cut(s) 215
HinfI GANTC 2 cut(s) 244, 271
HpaII CCGG 2 cut(s) 24, 185
Hpy166II GTNNAC 3 cut(s) 59, 168, 346
Hpy188I TCNGA 1 cut(s) 249
Hpy188III TCNNGA 4 cut(s) 7, 24, 140, 281
Hpy8I GTNNAC 3 cut(s) 59, 168, 346
Hpy99I CGWCG 1 cut(s) 306
HpyCH4IV ACGT 3 cut(s) 14, 304, 348
HpyCH4V TGCA 1 cut(s) 67
HpyF3I CTNAG 1 cut(s) 206
HpySE526I ACGT 3 cut(s) 14, 304, 348
Kpn2I TCCGGA 1 cut(s) 23
MaeII ACGT 3 cut(s) 14, 304, 348
MboII GAAGA 3 cut(s) 131, 253, 262
MluCI AATT 2 cut(s) 276, 319
MroI TCCGGA 1 cut(s) 23
MroXI GAANNNNTTC 1 cut(s) 257
MspI CCGG 2 cut(s) 24, 185
MspR9I CCNGG 2 cut(s) 185, 227
MvaI CCWGG 1 cut(s) 227
NciI CCSGG 1 cut(s) 185
PdmI GAANNNNTTC 1 cut(s) 257
PfeI GAWTC 2 cut(s) 244, 271
Ppu21I YACGTR 1 cut(s) 349
Psp6I CCWGG 1 cut(s) 225
PspGI CCWGG 1 cut(s) 225
RsaI GTAC 1 cut(s) 345
RsaNI GTAC 1 cut(s) 344
ScrFI CCNGG 2 cut(s) 185, 227
SetI ASST 8 cut(s) 17, 64, 182, 202, 219, 307, 316, 351
Sse9I AATT 2 cut(s) 276, 319
SspI AATATT 2 cut(s) 257, 337
StyD4I CCNGG 2 cut(s) 183, 225
StyI CCWWGG 1 cut(s) 49
TaiI ACGT 3 cut(s) 17, 307, 351
TaqI TCGA 2 cut(s) 155, 310
TasI AATT 2 cut(s) 276, 319
TfiI GAWTC 2 cut(s) 244, 271
TscAI CASTG 1 cut(s) 210
TspDTI ATGAA 1 cut(s) 132
TspRI CASTG 1 cut(s) 210
XapI RAATTY 1 cut(s) 276
XmnI GAANNNNTTC 1 cut(s) 257
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.