AT3G12760

Neddylation of cullins play an essential role in the regulation of SCF-type complexes activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0013210
Physical Location & Seq
Forward (+)
4054724 .. 4058840
4117 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G12760.1

Sequence Viewer

Length: 753 bp
ATGCATAAGTTGAGCAGAAGCAACCGTGACAAACTTCAGCAGTTCGTGGCTATCACAGGAGCTAGTGAGAAGAATGCTCTTCAGGCTCTCAAAGCCAGTGATTGGCACCTTGAAGCAGCGTTTGATGTGTTTTACAGCCAGCCTCAACCAAGAAGCAATGCTGCTGAAGTAAGACGCTTGGAGGAGCTCTACAATAGATATAAAGACCCGTATTCTGATATGATTCTTGCGGAGGGTATCTCGGTTCTGTGTAATGATCTTGAGGTGGAACCGCAAGACATAGTCACGTTGGTTCTTTCGTGGCATATGAATGCTGCAACAGCGTGTGAGTTTTCCAGGCAAGAATTTATCAGTGGATTACAGGCATTAGGTGTAGATTCAATCGGGAAGTTGCAGGAAAAGCTGCCATTTATGCGTTCTGAGCTTAAAGATGAACAAAAGTTCCATGAGATATACAACTTTGCTTTTGGGTGGGCGAAAGAGAAGGGGCAGAAATCTCTTGCCTTAGACACAGCGATTGGGATGTGGCAGTTGCTCTTTGCAGAAAGAGAGTGGCCTTTGGTAACTCACTGGTGTGATTTCTTGCAGGATCGTCATAACAAGGCCATATCTAAGGACACATGGGCACAGCTACTGGAATTTTCAAGGATGGTCGACCCAGTGCTGTCGAATTACGATGCAGAAGGAGCATGGCCTTACCTGATTGATGAATTCGTTGAGTATTTGTATGACAAAAATGTCGTTGAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cullin_binding UBA_4

Protein Analysis

250

Amino Acids

29.01

Weight (kDa)

5.0

Isoelectric Point (pI)

45.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UBA_8 PF22566 9 - 45 2.8e-11 UBA-like domain
UBA_4 PF14555 10 - 48 1e-13 UBA-like domain
Cullin_binding PF03556 130 - 241 1.8e-37 Cullin binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013210)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12760
fragaria_vesca FvH4_3g14590 FvH4_3g14590
malus_domestica MD05G1234900.v1.1 MD10G1211100.v1.1
prunus_persica Prupe.4G131300_v2.0.a1
pyrus_communis pycom05g21180 pycom10g17940
rosa_chinensis RchiOBHm_Chr5g0024051
rosa_laevigata RLG00000032757
rosa_multiflora Rmu_sc0011761.1_g000007
rosa_roxburghii Rroxscaffold_1G00055190
rosa_rugosa Rorug05G0081200 Rorug05G0081200
rosa_samantha Rh5AG171600 Rh5CG183800 Rh5DG170400
rosa_wichuraiana Rw5G015530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 737
AccB1I GGYRCC 1 cut(s) 105
AccB7I CCANNNNNTGG 1 cut(s) 102
AccI GTMKAC 1 cut(s) 654
AciI CCGC 2 cut(s) 230, 272
AclWI GGATC 1 cut(s) 597
AcsI RAATTY 3 cut(s) 344, 638, 710
AcuI CTGAAG 3 cut(s) 20, 65, 186
AfiI CCNNNNNNNGG 1 cut(s) 102
AgsI TTSAA 3 cut(s) 113, 381, 645
AjnI CCWGG 1 cut(s) 335
AleI CACNNNNGTG 1 cut(s) 573
AloI GAACNNNNNNTCC 2 cut(s) 426, 458
AluBI AGCT 5 cut(s) 62, 187, 403, 424, 631
AluI AGCT 5 cut(s) 62, 187, 403, 424, 631
Alw21I GWGCWC 1 cut(s) 189
AlwI GGATC 1 cut(s) 597
AlwNI CAGNNNCTG 1 cut(s) 634
AoxI GGCC 3 cut(s) 554, 603, 692
ApeKI GCWGC 4 cut(s) 116, 161, 314, 403
ApoI RAATTY 3 cut(s) 344, 638, 710
BaeGI GKGCMC 1 cut(s) 628
BanI GGYRCC 1 cut(s) 105
BanII GRGCYC 1 cut(s) 189
Bbv12I GWGCWC 1 cut(s) 189
BbvI GCAGC 4 cut(s) 128, 148, 301, 390
BccI CCATC 1 cut(s) 643
BciT130I CCWGG 1 cut(s) 337
BfaI CTAG 1 cut(s) 63
BisI GCNGC 4 cut(s) 117, 162, 315, 404
BlsI GCNGC 4 cut(s) 118, 163, 316, 405
Bme1390I CCNGG 1 cut(s) 337
BmiI GGNNCC 2 cut(s) 107, 270
BmrFI CCNGG 1 cut(s) 337
BmrI ACTGGG 1 cut(s) 653
BmsI GCATC 1 cut(s) 667
BmuI ACTGGG 1 cut(s) 653
BplI GAGNNNNNCTC 2 cut(s) 224, 256
BpuEI CTTGAG 1 cut(s) 281
Bsc4I CCNNNNNNNGG 1 cut(s) 102
Bse1I ACTGG 4 cut(s) 96, 575, 639, 659
Bse3DI GCAATG 1 cut(s) 163
BseBI CCWGG 1 cut(s) 337
BseGI GGATG 2 cut(s) 528, 654
BseLI CCNNNNNNNGG 1 cut(s) 102
BseMI GCAATG 1 cut(s) 163
BseMII CTCAG 1 cut(s) 411
BseNI ACTGG 4 cut(s) 96, 575, 639, 659
BseRI GAGGAG 1 cut(s) 197
BseSI GKGCMC 1 cut(s) 628
BseXI GCAGC 4 cut(s) 128, 148, 301, 390
BshFI GGCC 3 cut(s) 556, 605, 694
BshNI GGYRCC 1 cut(s) 105
BsiHKAI GWGCWC 1 cut(s) 189
BslI CCNNNNNNNGG 1 cut(s) 102
BsmI GAATGC 2 cut(s) 79, 316
BsnI GGCC 3 cut(s) 556, 605, 694
Bsp1286I GDGCHC 2 cut(s) 189, 628
Bsp143I GATC 2 cut(s) 256, 589
BspACI CCGC 2 cut(s) 230, 272
BspANI GGCC 3 cut(s) 556, 605, 694
BspCNI CTCAG 1 cut(s) 412
BspLI GGNNCC 2 cut(s) 107, 270
BspPI GGATC 1 cut(s) 597
BspQI GCTCTTC 1 cut(s) 84
BspT107I GGYRCC 1 cut(s) 105
BsrDI GCAATG 1 cut(s) 163
BsrI ACTGG 4 cut(s) 96, 575, 639, 659
BssMI GATC 2 cut(s) 256, 589
Bst2UI CCWGG 1 cut(s) 337
Bst4CI ACNGT 1 cut(s) 26
Bst6I CTCTTC 1 cut(s) 84
BstC8I GCNNGC 1 cut(s) 140
BstDEI CTNAG 3 cut(s) 420, 505, 612
BstF5I GGATG 2 cut(s) 528, 654
BstKTI GATC 2 cut(s) 259, 592
BstMBI GATC 2 cut(s) 256, 589
BstMWI GCNNNNNNNGC 7 cut(s) 83, 92, 320, 400, 412, 421, 686
BstNI CCWGG 1 cut(s) 337
BstSCI CCNGG 1 cut(s) 335
BstSLI GKGCMC 1 cut(s) 628
BstV1I GCAGC 4 cut(s) 128, 148, 301, 390
BsuRI GGCC 3 cut(s) 556, 605, 694
BtsCI GGATG 2 cut(s) 528, 654
BtsIMutI CAGTG 4 cut(s) 103, 358, 568, 666
Cac8I GCNNGC 1 cut(s) 140
CaiI CAGNNNCTG 1 cut(s) 634
CseI GACGC 1 cut(s) 183
CviAII CATG 3 cut(s) 446, 621, 690
DdeI CTNAG 3 cut(s) 420, 505, 612
DpnI GATC 2 cut(s) 258, 591
DpnII GATC 2 cut(s) 256, 589
DrdI GACNNNNNNGTC 1 cut(s) 737
DseDI GACNNNNNNGTC 1 cut(s) 737
Eam1104I CTCTTC 1 cut(s) 84
EarI CTCTTC 1 cut(s) 84
Ecl136II GAGCTC 1 cut(s) 187
Eco24I GRGCYC 1 cut(s) 189
Eco53kI GAGCTC 1 cut(s) 187
Eco57I CTGAAG 3 cut(s) 20, 65, 186
EcoICRI GAGCTC 1 cut(s) 187
EcoRI GAATTC 1 cut(s) 710
EcoRII CCWGG 1 cut(s) 335
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 189
FaeI CATG 3 cut(s) 449, 624, 693
FatI CATG 3 cut(s) 445, 620, 689
FauNDI CATATG 1 cut(s) 306
FblI GTMKAC 1 cut(s) 654
Fnu4HI GCNGC 4 cut(s) 117, 162, 315, 404
FokI GGATG 2 cut(s) 535, 661
FriOI GRGCYC 1 cut(s) 189
Fsp4HI GCNGC 4 cut(s) 117, 162, 315, 404
FspBI CTAG 1 cut(s) 63
GluI GCNGC 4 cut(s) 117, 162, 315, 404
HaeIII GGCC 3 cut(s) 556, 605, 694
HgaI GACGC 1 cut(s) 183
Hin1II CATG 3 cut(s) 449, 624, 693
HincII GTYRAC 1 cut(s) 655
HindII GTYRAC 1 cut(s) 655
HinfI GANTC 2 cut(s) 223, 377
Hpy166II GTNNAC 1 cut(s) 655
Hpy188I TCNGA 2 cut(s) 217, 421
Hpy188III TCNNGA 2 cut(s) 260, 385
Hpy8I GTNNAC 1 cut(s) 655
HpyAV CCTTC 2 cut(s) 478, 677
HpyCH4III ACNGT 1 cut(s) 26
HpyCH4IV ACGT 1 cut(s) 287
HpyCH4V TGCA 6 cut(s) 4, 317, 394, 542, 586, 680
HpyF10VI GCNNNNNNNGC 7 cut(s) 83, 92, 320, 400, 412, 421, 686
HpyF3I CTNAG 3 cut(s) 420, 505, 612
HpySE526I ACGT 1 cut(s) 287
Hsp92II CATG 3 cut(s) 449, 624, 693
Kzo9I GATC 2 cut(s) 256, 589
LguI GCTCTTC 1 cut(s) 84
LmnI GCTCC 3 cut(s) 59, 184, 686
Lsp1109I GCAGC 4 cut(s) 128, 148, 301, 390
LweI GCATC 1 cut(s) 667
MaeI CTAG 1 cut(s) 63
MaeII ACGT 1 cut(s) 287
MaeIII GTNAC 3 cut(s) 26, 283, 562
MalI GATC 2 cut(s) 258, 591
MboI GATC 2 cut(s) 256, 589
MboII GAAGA 2 cut(s) 71, 82
MhlI GDGCHC 2 cut(s) 189, 628
MluCI AATT 4 cut(s) 344, 638, 670, 710
MnlI CCTC 4 cut(s) 153, 175, 226, 256
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 1 cut(s) 426
MslI CAYNNNNRTG 2 cut(s) 309, 573
MspR9I CCNGG 1 cut(s) 337
Mva1269I GAATGC 2 cut(s) 79, 316
MvaI CCWGG 1 cut(s) 337
MwoI GCNNNNNNNGC 7 cut(s) 83, 92, 320, 400, 412, 421, 686
NdeI CATATG 1 cut(s) 306
NdeII GATC 2 cut(s) 256, 589
NlaIII CATG 3 cut(s) 449, 624, 693
NlaIV GGNNCC 2 cut(s) 107, 270
NmuCI GTSAC 2 cut(s) 26, 283
NsiI ATGCAT 1 cut(s) 6
OliI CACNNNNGTG 1 cut(s) 573
PciSI GCTCTTC 1 cut(s) 84
PctI GAATGC 2 cut(s) 79, 316
PfeI GAWTC 2 cut(s) 223, 377
PflFI GACNNNGTC 1 cut(s) 281
PflMI CCANNNNNTGG 1 cut(s) 102
PkrI GCNGC 4 cut(s) 118, 163, 316, 405
Psp124BI GAGCTC 1 cut(s) 189
Psp6I CCWGG 1 cut(s) 335
PspGI CCWGG 1 cut(s) 335
PspN4I GGNNCC 2 cut(s) 107, 270
PstNI CAGNNNCTG 1 cut(s) 634
PsyI GACNNNGTC 1 cut(s) 281
RseI CAYNNNNRTG 2 cut(s) 309, 573
SacI GAGCTC 1 cut(s) 189
SalI GTCGAC 1 cut(s) 653
SapI GCTCTTC 1 cut(s) 84
SaqAI TTAA 1 cut(s) 426
SatI GCNGC 4 cut(s) 117, 162, 315, 404
Sau3AI GATC 2 cut(s) 256, 589
ScrFI CCNGG 1 cut(s) 337
SduI GDGCHC 2 cut(s) 189, 628
SfaNI GCATC 1 cut(s) 667
SmiMI CAYNNNNRTG 2 cut(s) 309, 573
SmlI CTYRAG 1 cut(s) 260
SmoI CTYRAG 1 cut(s) 260
Sse9I AATT 4 cut(s) 344, 638, 670, 710
SsiI CCGC 2 cut(s) 230, 272
SspMI CTAG 1 cut(s) 63
SstI GAGCTC 1 cut(s) 189
StyD4I CCNGG 1 cut(s) 335
TaaI ACNGT 1 cut(s) 26
TaiI ACGT 1 cut(s) 290
TaqI TCGA 2 cut(s) 654, 668
TasI AATT 4 cut(s) 344, 638, 670, 710
TfiI GAWTC 2 cut(s) 223, 377
Tru1I TTAA 1 cut(s) 426
Tru9I TTAA 1 cut(s) 426
TscAI CASTG 4 cut(s) 103, 358, 575, 666
TseFI GTSAC 2 cut(s) 26, 283
TseI GCWGC 4 cut(s) 116, 161, 314, 403
Tsp45I GTSAC 2 cut(s) 26, 283
TspDTI ATGAA 3 cut(s) 323, 447, 723
TspRI CASTG 4 cut(s) 103, 358, 575, 666
Tth111I GACNNNGTC 1 cut(s) 281
Van91I CCANNNNNTGG 1 cut(s) 102
XapI RAATTY 3 cut(s) 344, 638, 710
XmiI GTMKAC 1 cut(s) 654
XspI CTAG 1 cut(s) 63
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.