Prupe.4G131300_v2.0.a1

Neddylation of cullins play an essential role in the regulation of SCF-type complexes activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0013210
Physical Location & Seq
Forward (+)
7248901 .. 7253995
5095 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G131300.1

Sequence Viewer

Length: 753 bp
ATGCACAAATTGGGTAGAGGCCACCGGGAAAAGCTCCAGCAGTTCATAACAATAACTGGAGCGAGTGAAAAAGTTGCTCTGCAGGCTTTGAAGTCGAGTGATTGGCATCTTGAAGGAGCATTTGATGTATTCTACAGTCAGCCCCAGTTGCAAACAAATACTGATTCTAGACATTTGGAGGAATTATACAATAGATATAAAGATCCATGTGCTGACATGATACTCGCTGATGGTATCAGCATCCTTTGCAACGATCTTCAGGTGGATCCTCAAGATATAGTCATGTTAGTTGTCTCCTGGCACATGAAGGCAGCCACCATGTGTGAATTTTCCAAGCAGGAGTTCATAGATGGATTAGAAACACTGGGGATAGATTCTCTGGAGAAATTCCGTGAAAAGATACCATTTATGCGATCTGAGCTGAAAGATGAACAAAAGTTTCGCGAGATATATAATTTTGCTTTTGGCTGGGCCAAAGAAAAGGGTCAGAAATCTTTGGCATTGGATACTGCAATTGGAATGTGGCAATTACTGTTTGCTGAAAAGCAGTGGCCGTTTGTCGATCATTGGTGTCAGTTCTTACAGGCTCGGCATAACAAGGCAATTTCAAGGGACACATGGTCTCAACTATTAGAGTTTGCAAGGGCTGTGGATTCATCACTCACAAATTATGACGCCGAAGGTGCCTGGCCCTATCTTATTGACGAATTTGTCGAGTACTTGAAGGAGAATGGTGTAATCCAAAAAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cullin_binding UBA_4

Protein Analysis

251

Amino Acids

29.09

Weight (kDa)

5.22

Isoelectric Point (pI)

35.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013210)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12760
fragaria_vesca FvH4_3g14590 FvH4_3g14590
malus_domestica MD05G1234900.v1.1 MD10G1211100.v1.1
prunus_persica Prupe.4G131300_v2.0.a1
pyrus_communis pycom05g21180 pycom10g17940
rosa_chinensis RchiOBHm_Chr5g0024051
rosa_laevigata RLG00000032757
rosa_multiflora Rmu_sc0011761.1_g000007
rosa_roxburghii Rroxscaffold_1G00055190
rosa_rugosa Rorug05G0081200 Rorug05G0081200
rosa_samantha Rh5AG171600 Rh5CG183800 Rh5DG170400
rosa_wichuraiana Rw5G015530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 710
AccB1I GGYRCC 1 cut(s) 683
AccII CGCG 1 cut(s) 444
AclWI GGATC 3 cut(s) 197, 260, 273
AcoI YGGCCR 1 cut(s) 551
AcsI RAATTY 3 cut(s) 326, 386, 707
AcuI CTGAAG 1 cut(s) 242
AcyI GRCGYC 1 cut(s) 675
AdeI CACNNNGTG 1 cut(s) 321
AfaI GTAC 1 cut(s) 719
AgsI TTSAA 4 cut(s) 91, 113, 609, 724
AhdI GACNNNNNGTC 1 cut(s) 619
AjnI CCWGG 2 cut(s) 296, 686
AjuI GAANNNNNNNTTGG 2 cut(s) 326, 358
AluBI AGCT 3 cut(s) 34, 421, 750
AluI AGCT 3 cut(s) 34, 421, 750
Alw26I GTCTC 2 cut(s) 298, 627
AlwI GGATC 3 cut(s) 197, 260, 273
AoxI GGCC 4 cut(s) 19, 471, 551, 689
ApeKI GCWGC 1 cut(s) 311
ApoI RAATTY 3 cut(s) 326, 386, 707
AspS9I GGNCC 2 cut(s) 471, 690
AsuC2I CCSGG 1 cut(s) 26
BamHI GGATCC 1 cut(s) 265
BanI GGYRCC 1 cut(s) 683
BbvI GCAGC 1 cut(s) 323
BccI CCATC 2 cut(s) 224, 344
BceAI ACGGC 1 cut(s) 538
BciT130I CCWGG 2 cut(s) 298, 688
BciVI GTATCC 1 cut(s) 499
BcnI CCSGG 1 cut(s) 26
BcoDI GTCTC 2 cut(s) 298, 627
BfaI CTAG 2 cut(s) 168, 751
BfmI CTRYAG 2 cut(s) 80, 133
BfuI GTATCC 1 cut(s) 499
BisI GCNGC 1 cut(s) 312
BlsI GCNGC 1 cut(s) 313
BmcAI AGTACT 1 cut(s) 719
Bme1390I CCNGG 3 cut(s) 26, 298, 688
BmeRI GACNNNNNGTC 1 cut(s) 619
BmgT120I GGNCC 2 cut(s) 471, 690
BmiI GGNNCC 2 cut(s) 267, 685
BmrFI CCNGG 3 cut(s) 26, 298, 688
BmrI ACTGGG 2 cut(s) 139, 374
BmsI GCATC 2 cut(s) 115, 249
BmuI ACTGGG 2 cut(s) 139, 374
BpmI CTGGAG 3 cut(s) 20, 78, 401
BpuEI CTTGAG 1 cut(s) 255
BpuMI CCSGG 1 cut(s) 26
BsaBI GATNNNNATC 1 cut(s) 105
BsaHI GRCGYC 1 cut(s) 675
BsaI GGTCTC 1 cut(s) 627
Bse1I ACTGG 3 cut(s) 61, 145, 369
Bse8I GATNNNNATC 1 cut(s) 105
BseBI CCWGG 2 cut(s) 298, 688
BseGI GGATG 1 cut(s) 240
BseJI GATNNNNATC 1 cut(s) 105
BseMII CTCAG 1 cut(s) 408
BseNI ACTGG 3 cut(s) 61, 145, 369
BseXI GCAGC 1 cut(s) 323
BseYI CCCAGC 1 cut(s) 468
Bsh1236I CGCG 1 cut(s) 444
BshFI GGCC 4 cut(s) 21, 473, 553, 691
BshNI GGYRCC 1 cut(s) 683
BsiSI CCGG 1 cut(s) 25
BslFI GGGAC 1 cut(s) 626
BsmAI GTCTC 2 cut(s) 298, 627
BsmFI GGGAC 1 cut(s) 626
BsnI GGCC 4 cut(s) 21, 473, 553, 691
Bso31I GGTCTC 1 cut(s) 627
Bsp143I GATC 5 cut(s) 202, 253, 265, 413, 562
Bsp68I TCGCGA 1 cut(s) 444
BspANI GGCC 4 cut(s) 21, 473, 553, 691
BspCNI CTCAG 1 cut(s) 409
BspFNI CGCG 1 cut(s) 444
BspLI GGNNCC 2 cut(s) 267, 685
BspMAI CTGCAG 1 cut(s) 84
BspPI GGATC 3 cut(s) 197, 260, 273
BspT107I GGYRCC 1 cut(s) 683
BspTNI GGTCTC 1 cut(s) 627
BsrI ACTGG 3 cut(s) 61, 145, 369
BssMI GATC 5 cut(s) 202, 253, 265, 413, 562
BssNI GRCGYC 1 cut(s) 675
Bst2UI CCWGG 2 cut(s) 298, 688
Bst4CI ACNGT 2 cut(s) 137, 534
BstACI GRCGYC 1 cut(s) 675
BstAPI GCANNNNNTGC 1 cut(s) 246
BstC8I GCNNGC 1 cut(s) 84
BstDEI CTNAG 1 cut(s) 417
BstF5I GGATG 1 cut(s) 240
BstFNI CGCG 1 cut(s) 444
BstKTI GATC 5 cut(s) 205, 256, 268, 416, 565
BstMAI GTCTC 2 cut(s) 298, 627
BstMBI GATC 5 cut(s) 202, 253, 265, 413, 562
BstMWI GCNNNNNNNGC 5 cut(s) 83, 148, 246, 418, 683
BstNI CCWGG 2 cut(s) 298, 688
BstSCI CCNGG 3 cut(s) 24, 296, 686
BstSFI CTRYAG 2 cut(s) 80, 133
BstUI CGCG 1 cut(s) 444
BstV1I GCAGC 1 cut(s) 323
BstX2I RGATCY 2 cut(s) 202, 265
BstYI RGATCY 2 cut(s) 202, 265
BsuI GTATCC 1 cut(s) 499
BsuRI GGCC 4 cut(s) 21, 473, 553, 691
BtsCI GGATG 1 cut(s) 240
BtsI GCAGTG 1 cut(s) 554
BtsIMutI CAGTG 2 cut(s) 362, 554
BtuMI TCGCGA 1 cut(s) 444
Cac8I GCNNGC 1 cut(s) 84
Cfr13I GGNCC 2 cut(s) 471, 690
CseI GACGC 1 cut(s) 683
Csp6I GTAC 1 cut(s) 718
CspCI CAANNNNNGTGG 2 cut(s) 630, 665
CviAII CATG 6 cut(s) 207, 217, 283, 304, 319, 618
CviQI GTAC 1 cut(s) 718
DdeI CTNAG 1 cut(s) 417
DpnI GATC 5 cut(s) 204, 255, 267, 415, 564
DpnII GATC 5 cut(s) 202, 253, 265, 413, 562
DraIII CACNNNGTG 1 cut(s) 321
DrdI GACNNNNNNGTC 1 cut(s) 710
DriI GACNNNNNGTC 1 cut(s) 619
DseDI GACNNNNNNGTC 1 cut(s) 710
EaeI YGGCCR 1 cut(s) 551
Eam1105I GACNNNNNGTC 1 cut(s) 619
Eco31I GGTCTC 1 cut(s) 627
Eco57I CTGAAG 1 cut(s) 242
EcoRII CCWGG 2 cut(s) 296, 686
FaeI CATG 6 cut(s) 210, 220, 286, 307, 322, 621
FaqI GGGAC 1 cut(s) 626
FatI CATG 6 cut(s) 206, 216, 282, 303, 318, 617
Fnu4HI GCNGC 1 cut(s) 312
FokI GGATG 1 cut(s) 227
Fsp4HI GCNGC 1 cut(s) 312
FspBI CTAG 2 cut(s) 168, 751
GluI GCNGC 1 cut(s) 312
GsaI CCCAGC 1 cut(s) 472
GsuI CTGGAG 3 cut(s) 20, 78, 401
HaeIII GGCC 4 cut(s) 21, 473, 553, 691
HapII CCGG 1 cut(s) 25
HgaI GACGC 1 cut(s) 683
Hin1I GRCGYC 1 cut(s) 675
Hin1II CATG 6 cut(s) 210, 220, 286, 307, 322, 621
HinfI GANTC 3 cut(s) 164, 374, 653
HpaII CCGG 1 cut(s) 25
Hpy188I TCNGA 2 cut(s) 418, 489
Hpy188III TCNNGA 5 cut(s) 110, 168, 272, 380, 443
HpyAV CCTTC 4 cut(s) 107, 301, 674, 718
HpyCH4III ACNGT 2 cut(s) 137, 534
HpyCH4V TGCA 6 cut(s) 4, 82, 151, 249, 512, 641
HpyF10VI GCNNNNNNNGC 5 cut(s) 83, 148, 246, 418, 683
HpyF3I CTNAG 1 cut(s) 417
Hsp92I GRCGYC 1 cut(s) 675
Hsp92II CATG 6 cut(s) 210, 220, 286, 307, 322, 621
Kzo9I GATC 5 cut(s) 202, 253, 265, 413, 562
LmnI GCTCC 3 cut(s) 39, 59, 116
Lsp1109I GCAGC 1 cut(s) 323
LweI GCATC 2 cut(s) 115, 249
MaeI CTAG 2 cut(s) 168, 751
MalI GATC 5 cut(s) 204, 255, 267, 415, 564
MboI GATC 5 cut(s) 202, 253, 265, 413, 562
MboII GAAGA 1 cut(s) 248
MfeI CAATTG 1 cut(s) 513
MflI RGATCY 2 cut(s) 202, 265
MnlI CCTC 3 cut(s) 11, 172, 279
MspI CCGG 1 cut(s) 25
MspR9I CCNGG 3 cut(s) 26, 298, 688
MunI CAATTG 1 cut(s) 513
MvaI CCWGG 2 cut(s) 298, 688
MvnI CGCG 1 cut(s) 444
MwoI GCNNNNNNNGC 5 cut(s) 83, 148, 246, 418, 683
NciI CCSGG 1 cut(s) 26
NdeII GATC 5 cut(s) 202, 253, 265, 413, 562
NlaIII CATG 6 cut(s) 210, 220, 286, 307, 322, 621
NlaIV GGNNCC 2 cut(s) 267, 685
NmeAIII GCCGAG 1 cut(s) 568
NruI TCGCGA 1 cut(s) 444
PcsI WCGNNNNNNNCGW 1 cut(s) 711
PfeI GAWTC 3 cut(s) 164, 374, 653
PkrI GCNGC 1 cut(s) 313
Psp6I CCWGG 2 cut(s) 296, 686
PspFI CCCAGC 1 cut(s) 468
PspGI CCWGG 2 cut(s) 296, 686
PspN4I GGNNCC 2 cut(s) 267, 685
PspPI GGNCC 2 cut(s) 471, 690
PstI CTGCAG 1 cut(s) 84
PsuI RGATCY 2 cut(s) 202, 265
RruI TCGCGA 1 cut(s) 444
RsaI GTAC 1 cut(s) 719
RsaNI GTAC 1 cut(s) 718
SatI GCNGC 1 cut(s) 312
Sau3AI GATC 5 cut(s) 202, 253, 265, 413, 562
Sau96I GGNCC 2 cut(s) 471, 690
ScaI AGTACT 1 cut(s) 719
ScrFI CCNGG 3 cut(s) 26, 298, 688
SetI ASST 5 cut(s) 36, 264, 423, 685, 752
SfaNI GCATC 2 cut(s) 115, 249
SfcI CTRYAG 2 cut(s) 80, 133
SmlI CTYRAG 1 cut(s) 270
SmoI CTYRAG 1 cut(s) 270
SspMI CTAG 2 cut(s) 168, 751
StyD4I CCNGG 3 cut(s) 24, 296, 686
TaaI ACNGT 2 cut(s) 137, 534
TaqI TCGA 3 cut(s) 95, 561, 714
TatI WGTACW 1 cut(s) 717
TfiI GAWTC 3 cut(s) 164, 374, 653
TscAI CASTG 2 cut(s) 369, 554
TseI GCWGC 1 cut(s) 311
TspDTI ATGAA 5 cut(s) 34, 320, 334, 444, 645
TspGWI ACGGA 1 cut(s) 380
TspRI CASTG 2 cut(s) 369, 554
XapI RAATTY 3 cut(s) 326, 386, 707
XbaI TCTAGA 1 cut(s) 167
XspI CTAG 2 cut(s) 168, 751
ZrmI AGTACT 1 cut(s) 719
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.