MD10G1211100.v1.1

Neddylation of cullins play an essential role in the regulation of SCF-type complexes activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0013210
Physical Location & Seq
Reverse (-)
31039467 .. 31044093
4627 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1211100.v1.1.491

Sequence Viewer

Length: 804 bp
ATGAACAAGTTGGGTAAAGGGCAACGCGATAAGCTCCAGCAGTTCATCATGATAACTGGAGCGAGTGAAAAAGTGGCTCTGCAGGTTCTGAAGGAGAGTGATTGGCATCTTGAAGGAGCGTTTGATATCTTCTACACTCAGCCCCAGCTGCAAGCAGAGACTGATTCTAATACTGATTCTAATACTAATTCTAGACAATTGGAGGAATTATACAATACATATAAAGGCAAGCTTTACCTTTCCATTCACTCTTATCCAGATGTTGATATGATACTTGTTGATGGTATCAGCCTCCTTTGCAATGATCTTCAGGTGGATCCTCAAGATATAGTCATGTTAGTTGTTTCTTGGCACATGAAGGCAGCTACCATGTGTGAATTTTCCAAAGAGGAGTTCGTAGAGGGATTAGAACCACTAGGGATAGATTCTTTGGACAAGTTTCGTGAAAAGATACCATTTATGAGATCCGAGCTGAAAGATGAACAAAAGTTTCGAGAGGTATATAATTTTGCTTTTGACTGGGCAAAAGAAAAGGGTCAGAAATCTTTGGCATTGGATACTGCAATTGGAATGTGGCAATTAGTGTTTGCTGAAAAGCAGTGGCCGTATGTTGAACATTGGTGTCAGTTCTTACCGGCTCGGCATAACAAGGCCATTTCGAGGGACACATGGTCTCAACTATTGGAGTTTGCAACGGCTGTGGATCCGTCACTGTCAAACTATGACGCGGAAGGTGCCTGGCCATATCTGATTGACGAATTTGTCGAGTACTTGAAGGAGAACGGAGCAATCCAAAAGGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cullin_binding UBA_4

Protein Analysis

268

Amino Acids

30.91

Weight (kDa)

4.74

Isoelectric Point (pI)

26.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UBA_8 PF22566 8 - 45 2.6e-09 UBA-like domain
UBA_4 PF14555 10 - 48 3.1e-11 UBA-like domain
Cullin_binding PF03556 147 - 257 4.5e-37 Cullin binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013210)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12760
fragaria_vesca FvH4_3g14590 FvH4_3g14590
malus_domestica MD05G1234900.v1.1 MD10G1211100.v1.1
prunus_persica Prupe.4G131300_v2.0.a1
pyrus_communis pycom05g21180 pycom10g17940
rosa_chinensis RchiOBHm_Chr5g0024051
rosa_laevigata RLG00000032757
rosa_multiflora Rmu_sc0011761.1_g000007
rosa_roxburghii Rroxscaffold_1G00055190
rosa_rugosa Rorug05G0081200 Rorug05G0081200
rosa_samantha Rh5AG171600 Rh5CG183800 Rh5DG170400
rosa_wichuraiana Rw5G015530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 761
Acc36I ACCTGC 1 cut(s) 73
AccB1I GGYRCC 1 cut(s) 734
AccII CGCG 2 cut(s) 27, 728
AciI CCGC 1 cut(s) 728
AclWI GGATC 5 cut(s) 311, 324, 459, 698, 711
AcoI YGGCCR 2 cut(s) 602, 740
AcsI RAATTY 2 cut(s) 377, 758
AcuI CTGAAG 2 cut(s) 110, 293
AfaI GTAC 1 cut(s) 770
AfiI CCNNNNNNNGG 1 cut(s) 660
AgsI TTSAA 3 cut(s) 113, 614, 775
AhdI GACNNNNNGTC 1 cut(s) 670
AjnI CCWGG 1 cut(s) 737
AjuI GAANNNNNNNTTGG 2 cut(s) 377, 409
AluBI AGCT 5 cut(s) 34, 148, 232, 365, 472
AluI AGCT 5 cut(s) 34, 148, 232, 365, 472
Alw26I GTCTC 2 cut(s) 152, 678
AlwI GGATC 5 cut(s) 311, 324, 459, 698, 711
AlwNI CAGNNNCTG 2 cut(s) 88, 161
AoxI GGCC 3 cut(s) 602, 651, 740
ApeKI GCWGC 2 cut(s) 148, 362
ApoI RAATTY 2 cut(s) 377, 758
BalI TGGCCA 1 cut(s) 742
BamHI GGATCC 2 cut(s) 316, 703
BanI GGYRCC 1 cut(s) 734
BbvI GCAGC 2 cut(s) 135, 374
BccI CCATC 1 cut(s) 275
BceAI ACGGC 2 cut(s) 589, 711
BciT130I CCWGG 1 cut(s) 739
BciVI GTATCC 1 cut(s) 550
BcoDI GTCTC 2 cut(s) 152, 678
BfaI CTAG 2 cut(s) 192, 416
BfmI CTRYAG 1 cut(s) 80
BfuAI ACCTGC 1 cut(s) 73
BfuI GTATCC 1 cut(s) 550
BisI GCNGC 2 cut(s) 149, 363
BlsI GCNGC 2 cut(s) 150, 364
BmcAI AGTACT 1 cut(s) 770
Bme1390I CCNGG 1 cut(s) 739
BmeRI GACNNNNNGTC 1 cut(s) 670
BmiI GGNNCC 3 cut(s) 318, 705, 736
BmrFI CCNGG 1 cut(s) 739
BmrI ACTGGG 1 cut(s) 529
BmsI GCATC 1 cut(s) 115
BmuI ACTGGG 1 cut(s) 529
BpmI CTGGAG 2 cut(s) 20, 78
BpuEI CTTGAG 1 cut(s) 306
BsaBI GATNNNNATC 1 cut(s) 105
BsaI GGTCTC 1 cut(s) 678
Bsc4I CCNNNNNNNGG 1 cut(s) 660
Bse118I RCCGGY 1 cut(s) 634
Bse1I ACTGG 2 cut(s) 61, 524
Bse3DI GCAATG 1 cut(s) 307
Bse8I GATNNNNATC 1 cut(s) 105
BseBI CCWGG 1 cut(s) 739
BseJI GATNNNNATC 1 cut(s) 105
BseLI CCNNNNNNNGG 1 cut(s) 660
BseMI GCAATG 1 cut(s) 307
BseMII CTCAG 1 cut(s) 152
BseNI ACTGG 2 cut(s) 61, 524
BseRI GAGGAG 1 cut(s) 404
BseXI GCAGC 2 cut(s) 135, 374
BseYI CCCAGC 1 cut(s) 144
Bsh1236I CGCG 2 cut(s) 27, 728
BshFI GGCC 3 cut(s) 604, 653, 742
BshNI GGYRCC 1 cut(s) 734
BsiSI CCGG 1 cut(s) 635
BslFI GGGAC 1 cut(s) 677
BslI CCNNNNNNNGG 1 cut(s) 660
BsmAI GTCTC 2 cut(s) 152, 678
BsmFI GGGAC 1 cut(s) 677
BsnI GGCC 3 cut(s) 604, 653, 742
Bso31I GGTCTC 1 cut(s) 678
Bsp143I GATC 4 cut(s) 304, 316, 464, 703
BspACI CCGC 1 cut(s) 728
BspANI GGCC 3 cut(s) 604, 653, 742
BspCNI CTCAG 1 cut(s) 151
BspFNI CGCG 2 cut(s) 27, 728
BspHI TCATGA 1 cut(s) 48
BspLI GGNNCC 3 cut(s) 318, 705, 736
BspMAI CTGCAG 1 cut(s) 84
BspMI ACCTGC 1 cut(s) 73
BspPI GGATC 5 cut(s) 311, 324, 459, 698, 711
BspT107I GGYRCC 1 cut(s) 734
BspTNI GGTCTC 1 cut(s) 678
BsrDI GCAATG 1 cut(s) 307
BsrFI RCCGGY 1 cut(s) 634
BsrI ACTGG 2 cut(s) 61, 524
BssAI RCCGGY 1 cut(s) 634
BssMI GATC 4 cut(s) 304, 316, 464, 703
Bst2UI CCWGG 1 cut(s) 739
Bst4CI ACNGT 1 cut(s) 714
BstC8I GCNNGC 2 cut(s) 153, 230
BstDEI CTNAG 1 cut(s) 138
BstFNI CGCG 2 cut(s) 27, 728
BstKTI GATC 4 cut(s) 307, 319, 467, 706
BstMAI GTCTC 2 cut(s) 152, 678
BstMBI GATC 4 cut(s) 304, 316, 464, 703
BstMWI GCNNNNNNNGC 3 cut(s) 148, 297, 734
BstNI CCWGG 1 cut(s) 739
BstSCI CCNGG 1 cut(s) 737
BstSFI CTRYAG 1 cut(s) 80
BstUI CGCG 2 cut(s) 27, 728
BstV1I GCAGC 2 cut(s) 135, 374
BstX2I RGATCY 3 cut(s) 316, 464, 703
BstYI RGATCY 3 cut(s) 316, 464, 703
BsuI GTATCC 1 cut(s) 550
BsuRI GGCC 3 cut(s) 604, 653, 742
BtsI GCAGTG 1 cut(s) 605
BtsIMutI CAGTG 2 cut(s) 605, 710
BveI ACCTGC 1 cut(s) 73
Cac8I GCNNGC 2 cut(s) 153, 230
CaiI CAGNNNCTG 2 cut(s) 88, 161
CciI TCATGA 1 cut(s) 48
Cfr10I RCCGGY 1 cut(s) 634
CseI GACGC 1 cut(s) 734
Csp6I GTAC 1 cut(s) 769
CspCI CAANNNNNGTGG 2 cut(s) 681, 716
CviAII CATG 5 cut(s) 49, 334, 355, 370, 669
CviQI GTAC 1 cut(s) 769
DdeI CTNAG 1 cut(s) 138
DpnI GATC 4 cut(s) 306, 318, 466, 705
DpnII GATC 4 cut(s) 304, 316, 464, 703
DrdI GACNNNNNNGTC 1 cut(s) 761
DriI GACNNNNNGTC 1 cut(s) 670
DseDI GACNNNNNNGTC 1 cut(s) 761
EaeI YGGCCR 2 cut(s) 602, 740
Eam1105I GACNNNNNGTC 1 cut(s) 670
Eco31I GGTCTC 1 cut(s) 678
Eco32I GATATC 1 cut(s) 127
Eco57I CTGAAG 2 cut(s) 110, 293
EcoRII CCWGG 1 cut(s) 737
EcoRV GATATC 1 cut(s) 127
FaeI CATG 5 cut(s) 52, 337, 358, 373, 672
FalI AAGNNNNNCTT 2 cut(s) 216, 248
FaqI GGGAC 1 cut(s) 677
FatI CATG 5 cut(s) 48, 333, 354, 369, 668
Fnu4HI GCNGC 2 cut(s) 149, 363
Fsp4HI GCNGC 2 cut(s) 149, 363
FspBI CTAG 2 cut(s) 192, 416
GluI GCNGC 2 cut(s) 149, 363
GsaI CCCAGC 1 cut(s) 148
GsuI CTGGAG 2 cut(s) 20, 78
HaeIII GGCC 3 cut(s) 604, 653, 742
HapII CCGG 1 cut(s) 635
HgaI GACGC 1 cut(s) 734
Hin1II CATG 5 cut(s) 52, 337, 358, 373, 672
HindIII AAGCTT 1 cut(s) 230
HinfI GANTC 3 cut(s) 164, 176, 425
HpaII CCGG 1 cut(s) 635
Hpy188I TCNGA 4 cut(s) 90, 469, 540, 750
Hpy188III TCNNGA 7 cut(s) 49, 110, 192, 257, 323, 443, 494
HpyAV CCTTC 5 cut(s) 85, 107, 352, 725, 769
HpyCH4III ACNGT 1 cut(s) 714
HpyCH4V TGCA 5 cut(s) 82, 151, 300, 563, 692
HpyF10VI GCNNNNNNNGC 3 cut(s) 148, 297, 734
HpyF3I CTNAG 1 cut(s) 138
Hsp92II CATG 5 cut(s) 52, 337, 358, 373, 672
Kzo9I GATC 4 cut(s) 304, 316, 464, 703
LmnI GCTCC 4 cut(s) 39, 59, 116, 785
Lsp1109I GCAGC 2 cut(s) 135, 374
LweI GCATC 1 cut(s) 115
MaeI CTAG 2 cut(s) 192, 416
MaeIII GTNAC 1 cut(s) 708
MalI GATC 4 cut(s) 306, 318, 466, 705
MboI GATC 4 cut(s) 304, 316, 464, 703
MboII GAAGA 2 cut(s) 121, 299
MfeI CAATTG 2 cut(s) 197, 564
MflI RGATCY 3 cut(s) 316, 464, 703
MlsI TGGCCA 1 cut(s) 742
MluCI AATT 8 cut(s) 187, 197, 206, 377, 505, 564, 578, 758
MluNI TGGCCA 1 cut(s) 742
MnlI CCTC 7 cut(s) 196, 302, 330, 382, 394, 490, 654
Mox20I TGGCCA 1 cut(s) 742
MscI TGGCCA 1 cut(s) 742
MseI TTAA 1 cut(s) 802
Msp20I TGGCCA 1 cut(s) 742
MspA1I CMGCKG 1 cut(s) 148
MspI CCGG 1 cut(s) 635
MspR9I CCNGG 1 cut(s) 739
MunI CAATTG 2 cut(s) 197, 564
MvaI CCWGG 1 cut(s) 739
MvnI CGCG 2 cut(s) 27, 728
MwoI GCNNNNNNNGC 3 cut(s) 148, 297, 734
NdeII GATC 4 cut(s) 304, 316, 464, 703
NlaIII CATG 5 cut(s) 52, 337, 358, 373, 672
NlaIV GGNNCC 3 cut(s) 318, 705, 736
NmeAIII GCCGAG 1 cut(s) 619
NmuCI GTSAC 1 cut(s) 708
PagI TCATGA 1 cut(s) 48
PcsI WCGNNNNNNNCGW 1 cut(s) 762
PfeI GAWTC 3 cut(s) 164, 176, 425
PkrI GCNGC 2 cut(s) 150, 364
Psp6I CCWGG 1 cut(s) 737
PspFI CCCAGC 1 cut(s) 144
PspGI CCWGG 1 cut(s) 737
PspN4I GGNNCC 3 cut(s) 318, 705, 736
PstI CTGCAG 1 cut(s) 84
PstNI CAGNNNCTG 2 cut(s) 88, 161
PsuI RGATCY 3 cut(s) 316, 464, 703
PvuII CAGCTG 1 cut(s) 148
RsaI GTAC 1 cut(s) 770
RsaNI GTAC 1 cut(s) 769
SaqAI TTAA 1 cut(s) 802
SatI GCNGC 2 cut(s) 149, 363
Sau3AI GATC 4 cut(s) 304, 316, 464, 703
ScaI AGTACT 1 cut(s) 770
ScrFI CCNGG 1 cut(s) 739
SfaNI GCATC 1 cut(s) 115
SfcI CTRYAG 1 cut(s) 80
SmlI CTYRAG 1 cut(s) 321
SmoI CTYRAG 1 cut(s) 321
Sse9I AATT 8 cut(s) 187, 197, 206, 377, 505, 564, 578, 758
SsiI CCGC 1 cut(s) 728
SspMI CTAG 2 cut(s) 192, 416
StyD4I CCNGG 1 cut(s) 737
TaaI ACNGT 1 cut(s) 714
TaqI TCGA 3 cut(s) 493, 659, 765
TasI AATT 8 cut(s) 187, 197, 206, 377, 505, 564, 578, 758
TatI WGTACW 1 cut(s) 768
TfiI GAWTC 3 cut(s) 164, 176, 425
Tru1I TTAA 1 cut(s) 802
Tru9I TTAA 1 cut(s) 802
TscAI CASTG 2 cut(s) 605, 717
TseFI GTSAC 1 cut(s) 708
TseI GCWGC 2 cut(s) 148, 362
Tsp45I GTSAC 1 cut(s) 708
TspDTI ATGAA 4 cut(s) 17, 34, 371, 495
TspGWI ACGGA 2 cut(s) 696, 798
TspRI CASTG 2 cut(s) 605, 717
XapI RAATTY 2 cut(s) 377, 758
XbaI TCTAGA 1 cut(s) 191
XspI CTAG 2 cut(s) 192, 416
ZrmI AGTACT 1 cut(s) 770
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.