Rorug05G0081200

Neddylation of cullins play an essential role in the regulation of SCF-type complexes activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001629
Physical Location & Seq
Reverse (-)
7029373 .. 7033585
4213 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0081200.1

Sequence Viewer

Length: 1155 bp
ATGATGAATGAGCTGGAGTTAATTGGTGATCAGAAAGAAAAGACTGACAAAAATATTAGTGAGGACCAAGCAGAAAACATATCGGTGGGTTCTTCCCAGAAATGCTCATGCTTTGATTTAAATGAAGAAGCTAGCGATAACAGCGACAATGATATTAGCAATGCCAACGTTAACCTGGCTGATCATGAACTTAGTACAGTTAGAAATGATGAAAGAAGAGCCCTAGCCCAGGGAAACATTAATAGTAATGCAGCTAATAGTAGTAATAGCTCTAGGGTTAAAGTAAGGCCATATGTACGATCCAAACTACCTCGTCTGCGGTGGACACCCCAACTCCATCTTTCATTTTTGCATGCTTTGGAATTACTGGGAGGGCATGAGAGAGCAACTCCCAAGTTGGTTCTTCAGCTAATGAATGTGAGAGGACTAAGCATTTCCCACGTAAAGAGTCACTTGCAGATGTATCGAAGTAAGAAGCTTGATAGGGATGGTCAAGTAGTATCTGATGAGAAAACTTACGACTACACGGATGGAAGAGAATCAACGGATCACATACCTAACTTCGTTTCGAGGTCAAGTTCTCTGACAAAGAAAGAAGTCGTACAATGCAACAATTTGAACAAAGCTTTGCATCATACAAGCACTACTCCCATAAACCCTAGCCGATTACATGAGGAAAAGAGGTGGCCTCCATTTCAAAGCATTAGCAAAAGCTGGAGGGCCAGAAGATTTGCTTCCAACAATATCAATTGGTCAAATAGTCATAACGGCTCATCCCAATCTGATATAATGCATCATATGATTAGCAACAGACCAACAGATCATGATCTACGTTGTTGGAACCTTGAAGGTCATAGCAATTCAAGTAGCTACAAGCAAGAATTCGAACCGCCATTTAGGTTTGAGTGGAACAAGGAAAAAATTTTGAAGTACAAGGAATGGATGCCTGATTTGGAGTTGGGTCTGCAGAGCCAAAGAGTTTGGAATGAGATCAATGACGAGACTCAGCATCATCAGACGATTAGTCACGCAGAAGAACATTATGAAACTGTGCAAGGAGTTATAGAGACTTCACAACGTCACAAAGATCATCAATTCGAGGACATGAATAGCAAACAAGAAACTATTAGCACCAAGCTTTCCCTTTCTCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cullin_binding UBA_4

Protein Analysis

384

Amino Acids

44.38

Weight (kDa)

6.5

Isoelectric Point (pI)

58.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 105 - 156 3.7e-07 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 319, 890
AclI AACGTT 1 cut(s) 168
AclWI GGATC 2 cut(s) 294, 555
AcsI RAATTY 2 cut(s) 881, 921
AcuI CTGAAG 1 cut(s) 389
AfaI GTAC 4 cut(s) 196, 297, 603, 932
AfiI CCNNNNNNNGG 1 cut(s) 229
AgsI TTSAA 5 cut(s) 619, 698, 848, 864, 928
AhdI GACNNNNNGTC 1 cut(s) 1023
AjnI CCWGG 2 cut(s) 174, 228
Alw26I GTCTC 2 cut(s) 995, 1061
AlwI GGATC 2 cut(s) 294, 555
AoxI GGCC 3 cut(s) 287, 686, 720
ApeKI GCWGC 1 cut(s) 251
ApoI RAATTY 2 cut(s) 881, 921
AseI ATTAAT 1 cut(s) 240
AspS9I GGNCC 2 cut(s) 64, 720
AsuHPI GGTGA 1 cut(s) 38
AsuII TTCGAA 1 cut(s) 885
AsuNHI GCTAGC 1 cut(s) 131
AvaII GGWCC 1 cut(s) 64
BanII GRGCYC 1 cut(s) 223
BbvI GCAGC 1 cut(s) 263
BccI CCATC 3 cut(s) 345, 482, 524
BceAI ACGGC 1 cut(s) 784
BcgI CGANNNNNNTGC 2 cut(s) 446, 480
BciT130I CCWGG 2 cut(s) 176, 230
BclI TGATCA 2 cut(s) 28, 181
BcoDI GTCTC 2 cut(s) 995, 1061
BfaI CTAG 4 cut(s) 132, 224, 273, 660
BfmI CTRYAG 1 cut(s) 965
BisI GCNGC 1 cut(s) 252
BlsI GCNGC 1 cut(s) 253
Bme1390I CCNGG 2 cut(s) 176, 230
Bme18I GGWCC 1 cut(s) 64
BmeRI GACNNNNNGTC 1 cut(s) 1023
BmgT120I GGNCC 2 cut(s) 64, 720
BmiI GGNNCC 1 cut(s) 842
BmrFI CCNGG 2 cut(s) 176, 230
BmrI ACTGGG 1 cut(s) 377
BmsI GCATC 4 cut(s) 640, 802, 933, 1018
BmtI GCTAGC 1 cut(s) 135
BmuI ACTGGG 1 cut(s) 377
BplI GAGNNNNNCTC 4 cut(s) 373, 405, 673, 705
BpmI CTGGAG 2 cut(s) 35, 736
Bpu14I TTCGAA 1 cut(s) 885
BsaAI YACGTR 1 cut(s) 442
BsaBI GATNNNNATC 1 cut(s) 825
BsaJI CCNNGG 2 cut(s) 228, 229
BsaXI ACNNNNNCTCC 2 cut(s) 318, 348
Bsc4I CCNNNNNNNGG 1 cut(s) 229
Bse1I ACTGG 1 cut(s) 372
Bse3DI GCAATG 1 cut(s) 166
Bse8I GATNNNNATC 1 cut(s) 825
BseBI CCWGG 2 cut(s) 176, 230
BseDI CCNNGG 2 cut(s) 228, 229
BseGI GGATG 4 cut(s) 493, 535, 773, 948
BseJI GATNNNNATC 1 cut(s) 825
BseLI CCNNNNNNNGG 1 cut(s) 229
BseMI GCAATG 1 cut(s) 166
BseMII CTCAG 1 cut(s) 1019
BseNI ACTGG 1 cut(s) 372
BseXI GCAGC 1 cut(s) 263
BshFI GGCC 3 cut(s) 289, 688, 722
BslI CCNNNNNNNGG 1 cut(s) 229
BsmAI GTCTC 2 cut(s) 995, 1061
BsnI GGCC 3 cut(s) 289, 688, 722
Bsp119I TTCGAA 1 cut(s) 885
Bsp1286I GDGCHC 1 cut(s) 223
Bsp143I GATC 8 cut(s) 28, 181, 299, 547, 820, 826, 990, 1087
BspACI CCGC 2 cut(s) 319, 890
BspANI GGCC 3 cut(s) 289, 688, 722
BspCNI CTCAG 1 cut(s) 1018
BspHI TCATGA 2 cut(s) 184, 823
BspLI GGNNCC 1 cut(s) 842
BspMAI CTGCAG 1 cut(s) 969
BspOI GCTAGC 1 cut(s) 135
BspPI GGATC 2 cut(s) 294, 555
BspQI GCTCTTC 1 cut(s) 211
BspT104I TTCGAA 1 cut(s) 885
BsrDI GCAATG 1 cut(s) 166
BsrI ACTGG 1 cut(s) 372
BssECI CCNNGG 2 cut(s) 228, 229
BssMI GATC 8 cut(s) 28, 181, 299, 547, 820, 826, 990, 1087
Bst2UI CCWGG 2 cut(s) 176, 230
Bst4CI ACNGT 2 cut(s) 199, 1051
Bst6I CTCTTC 2 cut(s) 211, 529
BstBAI YACGTR 1 cut(s) 442
BstBI TTCGAA 1 cut(s) 885
BstC8I GCNNGC 2 cut(s) 133, 354
BstDEI CTNAG 3 cut(s) 191, 428, 1005
BstENI CCTNNNNNAGG 1 cut(s) 227
BstF5I GGATG 4 cut(s) 493, 535, 773, 948
BstKTI GATC 8 cut(s) 31, 184, 302, 550, 823, 829, 993, 1090
BstMAI GTCTC 2 cut(s) 995, 1061
BstMBI GATC 8 cut(s) 28, 181, 299, 547, 820, 826, 990, 1087
BstMWI GCNNNNNNNGC 1 cut(s) 141
BstNI CCWGG 2 cut(s) 176, 230
BstNSI RCATGY 1 cut(s) 356
BstSCI CCNGG 2 cut(s) 174, 228
BstSFI CTRYAG 1 cut(s) 965
BstV1I GCAGC 1 cut(s) 263
BsuRI GGCC 3 cut(s) 289, 688, 722
BtsCI GGATG 4 cut(s) 493, 535, 773, 948
Cac8I GCNNGC 2 cut(s) 133, 354
CciI TCATGA 2 cut(s) 184, 823
Cfr13I GGNCC 2 cut(s) 64, 720
Csp6I GTAC 4 cut(s) 195, 296, 602, 931
CviAII CATG 7 cut(s) 108, 185, 353, 377, 671, 824, 1105
CviQI GTAC 4 cut(s) 195, 296, 602, 931
DdeI CTNAG 3 cut(s) 191, 428, 1005
DpnI GATC 8 cut(s) 30, 183, 301, 549, 822, 828, 992, 1089
DpnII GATC 8 cut(s) 28, 181, 299, 547, 820, 826, 990, 1087
DraI TTTAAA 1 cut(s) 120
DriI GACNNNNNGTC 1 cut(s) 1023
Eam1104I CTCTTC 2 cut(s) 211, 529
Eam1105I GACNNNNNGTC 1 cut(s) 1023
EarI CTCTTC 2 cut(s) 211, 529
Eco24I GRGCYC 1 cut(s) 223
Eco47I GGWCC 1 cut(s) 64
Eco57I CTGAAG 1 cut(s) 389
EcoNI CCTNNNNNAGG 1 cut(s) 227
EcoRI GAATTC 1 cut(s) 881
EcoRII CCWGG 2 cut(s) 174, 228
EcoT22I ATGCAT 1 cut(s) 795
EcoT38I GRGCYC 1 cut(s) 223
FaeI CATG 7 cut(s) 111, 188, 356, 380, 674, 827, 1108
FalI AAGNNNNNCTT 4 cut(s) 437, 469, 718, 750
FatI CATG 7 cut(s) 107, 184, 352, 376, 670, 823, 1104
FauNDI CATATG 2 cut(s) 292, 798
FbaI TGATCA 2 cut(s) 28, 181
Fnu4HI GCNGC 1 cut(s) 252
FokI GGATG 4 cut(s) 500, 542, 760, 955
FriOI GRGCYC 1 cut(s) 223
Fsp4HI GCNGC 1 cut(s) 252
FspBI CTAG 4 cut(s) 132, 224, 273, 660
GluI GCNGC 1 cut(s) 252
GsuI CTGGAG 2 cut(s) 35, 736
HaeIII GGCC 3 cut(s) 289, 688, 722
Hin1II CATG 7 cut(s) 111, 188, 356, 380, 674, 827, 1108
HincII GTYRAC 1 cut(s) 172
HindII GTYRAC 1 cut(s) 172
HindIII AAGCTT 3 cut(s) 476, 624, 1136
HinfI GANTC 3 cut(s) 448, 539, 1003
HpaI GTTAAC 1 cut(s) 172
HphI GGTGA 1 cut(s) 38
Hpy166II GTNNAC 2 cut(s) 172, 324
Hpy188I TCNGA 5 cut(s) 33, 505, 585, 784, 1017
Hpy188III TCNNGA 2 cut(s) 185, 824
Hpy8I GTNNAC 2 cut(s) 172, 324
HpyAV CCTTC 1 cut(s) 842
HpyCH4III ACNGT 2 cut(s) 199, 1051
HpyCH4IV ACGT 4 cut(s) 168, 441, 832, 1078
HpyCH4V TGCA 8 cut(s) 251, 352, 457, 609, 631, 793, 967, 1054
HpyF10VI GCNNNNNNNGC 1 cut(s) 141
HpyF3I CTNAG 3 cut(s) 191, 428, 1005
HpySE526I ACGT 4 cut(s) 168, 441, 832, 1078
Hsp92II CATG 7 cut(s) 111, 188, 356, 380, 674, 827, 1108
Ksp22I TGATCA 2 cut(s) 28, 181
KspAI GTTAAC 1 cut(s) 172
Kzo9I GATC 8 cut(s) 28, 181, 299, 547, 820, 826, 990, 1087
LguI GCTCTTC 1 cut(s) 211
LpnPI CCDG 9 cut(s) 110, 161, 188, 215, 242, 353, 700, 736, 960
Lsp1109I GCAGC 1 cut(s) 263
LweI GCATC 4 cut(s) 640, 802, 933, 1018
MaeI CTAG 4 cut(s) 132, 224, 273, 660
MaeII ACGT 4 cut(s) 168, 441, 832, 1078
MaeIII GTNAC 3 cut(s) 449, 1025, 1079
MalI GATC 8 cut(s) 30, 183, 301, 549, 822, 828, 992, 1089
MboI GATC 8 cut(s) 28, 181, 299, 547, 820, 826, 990, 1087
MboII GAAGA 7 cut(s) 84, 137, 228, 395, 546, 738, 1046
MfeI CAATTG 1 cut(s) 748
MhlI GDGCHC 1 cut(s) 223
MluCI AATT 8 cut(s) 21, 362, 613, 748, 859, 881, 921, 1094
MlyI GAGTC 2 cut(s) 457, 997
MmeI TCCRAC 2 cut(s) 762, 818
Mph1103I ATGCAT 1 cut(s) 795
MseI TTAA 6 cut(s) 20, 119, 171, 240, 279, 1153
MslI CAYNNNNRTG 1 cut(s) 83
MspR9I CCNGG 2 cut(s) 176, 230
MunI CAATTG 1 cut(s) 748
MvaI CCWGG 2 cut(s) 176, 230
MwoI GCNNNNNNNGC 1 cut(s) 141
NdeI CATATG 2 cut(s) 292, 798
NdeII GATC 8 cut(s) 28, 181, 299, 547, 820, 826, 990, 1087
NheI GCTAGC 1 cut(s) 131
NlaIII CATG 7 cut(s) 111, 188, 356, 380, 674, 827, 1108
NlaIV GGNNCC 1 cut(s) 842
NmuCI GTSAC 3 cut(s) 449, 1025, 1079
NsiI ATGCAT 1 cut(s) 795
NspI RCATGY 1 cut(s) 356
NspV TTCGAA 1 cut(s) 885
PaeI GCATGC 1 cut(s) 356
PagI TCATGA 2 cut(s) 184, 823
PasI CCCWGGG 1 cut(s) 229
PciSI GCTCTTC 1 cut(s) 211
PfeI GAWTC 1 cut(s) 539
PkrI GCNGC 1 cut(s) 253
PleI GAGTC 2 cut(s) 456, 997
PpsI GAGTC 2 cut(s) 456, 997
Ppu21I YACGTR 1 cut(s) 442
PshBI ATTAAT 1 cut(s) 240
Psp1406I AACGTT 1 cut(s) 168
Psp6I CCWGG 2 cut(s) 174, 228
PspGI CCWGG 2 cut(s) 174, 228
PspN4I GGNNCC 1 cut(s) 842
PspPI GGNCC 2 cut(s) 64, 720
PstI CTGCAG 1 cut(s) 969
RsaI GTAC 4 cut(s) 196, 297, 603, 932
RsaNI GTAC 4 cut(s) 195, 296, 602, 931
RseI CAYNNNNRTG 1 cut(s) 83
SapI GCTCTTC 1 cut(s) 211
SaqAI TTAA 6 cut(s) 20, 119, 171, 240, 279, 1153
SatI GCNGC 1 cut(s) 252
Sau3AI GATC 8 cut(s) 28, 181, 299, 547, 820, 826, 990, 1087
Sau96I GGNCC 2 cut(s) 64, 720
SchI GAGTC 2 cut(s) 457, 997
ScrFI CCNGG 2 cut(s) 176, 230
SduI GDGCHC 1 cut(s) 223
SfaNI GCATC 4 cut(s) 640, 802, 933, 1018
SfcI CTRYAG 1 cut(s) 965
SfuI TTCGAA 1 cut(s) 885
SinI GGWCC 1 cut(s) 64
SmiI ATTTAAAT 1 cut(s) 120
SmiMI CAYNNNNRTG 1 cut(s) 83
SphI GCATGC 1 cut(s) 356
Sse9I AATT 8 cut(s) 21, 362, 613, 748, 859, 881, 921, 1094
SsiI CCGC 2 cut(s) 319, 890
SspI AATATT 1 cut(s) 55
SspMI CTAG 4 cut(s) 132, 224, 273, 660
StyD4I CCNGG 2 cut(s) 174, 228
SwaI ATTTAAAT 1 cut(s) 120
TaaI ACNGT 2 cut(s) 199, 1051
TaiI ACGT 4 cut(s) 171, 444, 835, 1081
TaqI TCGA 4 cut(s) 466, 569, 885, 1098
TasI AATT 8 cut(s) 21, 362, 613, 748, 859, 881, 921, 1094
TatI WGTACW 2 cut(s) 194, 930
TfiI GAWTC 1 cut(s) 539
Tru1I TTAA 6 cut(s) 20, 119, 171, 240, 279, 1153
Tru9I TTAA 6 cut(s) 20, 119, 171, 240, 279, 1153
TseFI GTSAC 3 cut(s) 449, 1025, 1079
TseI GCWGC 1 cut(s) 251
Tsp45I GTSAC 3 cut(s) 449, 1025, 1079
TspDTI ATGAA 8 cut(s) 20, 138, 201, 225, 333, 428, 1059, 1121
TspGWI ACGGA 2 cut(s) 542, 560
VpaK11BI GGWCC 1 cut(s) 64
VspI ATTAAT 1 cut(s) 240
XagI CCTNNNNNAGG 1 cut(s) 227
XapI RAATTY 2 cut(s) 881, 921
XceI RCATGY 1 cut(s) 356
XcmI CCANNNNNNNNNTGG 1 cut(s) 172
XspI CTAG 4 cut(s) 132, 224, 273, 660
Zsp2I ATGCAT 1 cut(s) 795
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.