MD05G1235000.v1.1

Metal tolerance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0001629
Physical Location & Seq
Forward (+)
36708685 .. 36712224
3540 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1235000.v1.1.491

Sequence Viewer

Length: 1215 bp
ATGGTGGCGGATCTTCGAACAGAATCTTCGGATTACCGGACCGAGCTCTTGTCGCCGGACGCGACGGGAGAGACTGTGACAGTGCCAACTGAGGCATCGTGGAGGCTCAACATGGATGGATTCAACCTTCCAGCTGAGAGAAGCATGGATTCTTACTTTGGCCTTGGCTCTTTTGTAAACTCATTAAGGAGGCAGAGAAAAATTGCGCAATATTACAAGAGGCAGAATAAGCTTCTCAAAGGATTCAACGAGATAGACTCATTTTCTGAGATGGGTTTTTGGCCTGGAAGTTTAACACAGGATGAGGTGAACCAACTTGCAAGAAATGAAAGGATGGCAATATATACATCTAATGCGGCTAACTTGGTGCTTTTCCTGGCAAAAGTTTATGCTTCAGTTGAGAGCAGATCTTTGGCAGTAATTGCTTCAACTTTGGATTCTCTCTTAGACCTCTTGTCCGGCTTCATTCTTTGGTTCACTTCTCATGCAATGAGAAAACCTAATCAGTATCGGTACCCGATTGGTAAAACCCGGATGCAACCTGTGGGAATTGTTGTTTTTGCATCTGTAATGGCAACTCTTGGACTACAAATATTGTTCGAATCTGGGCGGCAACTTCTCACAAAGACTCAACCTGATATGGATCCAGTGAAAGAAAAATGGATGATTGGGATAATGGTGTCTGCAACAGTTGTGAAGTTTGTGTTGATGGTGTATTGTCGTAGATTCAAGAACGAAATTGTTCGAGCTTACGCTCAAGATCATCTGTTTGATGTCATCACTAACGGAATTGGTCTTGCAGCTGCAGTTTTAGCAATCAGGTTTTACTGGTGGCTTGATCCTGTTGGAGCTATCATTATTGCTTTGTACACAATGGGGAACTGGGCGAAAACAGTGATGGATAATGTGTGGTCACTGATAGGGAAGACAGCACCAGCAGAGTACTTGGCAAAATTGACATATCTGATATGGAACCATGACAAGGAGATCACGCACATTGAGACAGTGAGAGCATACACTTTTGGCTGCAACTATTTTGTGGAGGTTCACATTGTCTTGTCCGGAGACATGTCTCTCAGCCACGCACATAACATCGGAGAGACGCTTCAGGAAAAGATCGAAAACCTTCCTGAGGTGGAACGAGCTTTCGTTCATGTCGACTTTGACACTACTCACAGGCCTGAGCACAACAATGCTAAGTTAGCACCATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cation_efflux ZT_dimer

Protein Analysis

405

Amino Acids

45.85

Weight (kDa)

6.57

Isoelectric Point (pI)

39.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_efflux PF01545 117 - 306 9.5e-33 Cation efflux transmembrane domain
ZT_dimer PF16916 321 - 387 5.8e-09 Cation efflux protein, cytoplasmic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 207
Acc65I GGTACC 1 cut(s) 513
AccB1I GGYRCC 1 cut(s) 513
AccI GTMKAC 1 cut(s) 1158
AccII CGCG 1 cut(s) 62
AccIII TCCGGA 1 cut(s) 1061
AciI CCGC 3 cut(s) 8, 356, 610
AclWI GGATC 4 cut(s) 18, 638, 651, 833
AcuI CTGAAG 2 cut(s) 378, 1091
AfaI GTAC 3 cut(s) 515, 869, 944
AfiI CCNNNNNNNGG 2 cut(s) 982, 1132
AflIII ACRYGT 1 cut(s) 1068
AgsI TTSAA 4 cut(s) 124, 247, 429, 730
AhdI GACNNNNNGTC 1 cut(s) 454
AjnI CCWGG 2 cut(s) 283, 375
AluBI AGCT 7 cut(s) 46, 134, 232, 749, 803, 851, 1145
AluI AGCT 7 cut(s) 46, 134, 232, 749, 803, 851, 1145
Alw21I GWGCWC 2 cut(s) 48, 1188
Alw26I GTCTC 5 cut(s) 65, 995, 1059, 1077, 1094
AlwI GGATC 4 cut(s) 18, 638, 651, 833
Aor13HI TCCGGA 1 cut(s) 1061
AoxI GGCC 3 cut(s) 160, 281, 1178
ApeKI GCWGC 3 cut(s) 800, 803, 1026
Asp700I GAANNNNTTC 2 cut(s) 741, 1125
Asp718I GGTACC 1 cut(s) 513
AspLEI GCGC 1 cut(s) 208
AspS9I GGNCC 1 cut(s) 39
AsuC2I CCSGG 1 cut(s) 532
AsuHPI GGTGA 1 cut(s) 319
AsuII TTCGAA 2 cut(s) 16, 600
AvaII GGWCC 1 cut(s) 39
AxyI CCTNAGG 1 cut(s) 1131
BamHI GGATCC 1 cut(s) 643
BanI GGYRCC 1 cut(s) 513
BanII GRGCYC 1 cut(s) 48
BbsI GAAGAC 1 cut(s) 932
Bbv12I GWGCWC 2 cut(s) 48, 1188
BbvI GCAGC 3 cut(s) 790, 812, 1013
BccI CCATC 5 cut(s) 110, 265, 328, 703, 892
BciT130I CCWGG 2 cut(s) 285, 377
BcnI CCSGG 1 cut(s) 532
BcoDI GTCTC 5 cut(s) 65, 995, 1059, 1077, 1094
BfmI CTRYAG 1 cut(s) 804
BglII AGATCT 1 cut(s) 407
BisI GCNGC 5 cut(s) 357, 611, 801, 804, 1027
BlsI GCNGC 5 cut(s) 358, 612, 802, 805, 1028
BmcAI AGTACT 1 cut(s) 944
Bme1390I CCNGG 3 cut(s) 285, 377, 532
Bme18I GGWCC 1 cut(s) 39
BmeRI GACNNNNNGTC 1 cut(s) 454
BmgT120I GGNCC 1 cut(s) 39
BmiI GGNNCC 3 cut(s) 515, 645, 974
BmrFI CCNGG 3 cut(s) 285, 377, 532
BmrI ACTGGG 1 cut(s) 892
BmsI GCATC 3 cut(s) 104, 525, 572
BmuI ACTGGG 1 cut(s) 892
BpiI GAAGAC 1 cut(s) 932
BplI GAGNNNNNCTC 2 cut(s) 242, 274
Bpu10I CCTNAGC 1 cut(s) 1182
Bpu14I TTCGAA 2 cut(s) 16, 600
BpuEI CTTGAG 1 cut(s) 741
BpuMI CCSGG 1 cut(s) 532
BsaBI GATNNNNATC 1 cut(s) 642
BsaJI CCNNGG 1 cut(s) 163
BsaWI WCCGGW 2 cut(s) 36, 1061
BsaXI ACNNNNNCTCC 2 cut(s) 60, 90
Bsc4I CCNNNNNNNGG 2 cut(s) 982, 1132
Bse1I ACTGG 3 cut(s) 647, 833, 887
Bse21I CCTNAGG 1 cut(s) 1131
Bse3DI GCAATG 1 cut(s) 495
Bse8I GATNNNNATC 1 cut(s) 642
BseAI TCCGGA 1 cut(s) 1061
BseBI CCWGG 2 cut(s) 285, 377
BseDI CCNNGG 1 cut(s) 163
BseGI GGATG 5 cut(s) 121, 307, 339, 540, 669
BseJI GATNNNNATC 1 cut(s) 642
BseLI CCNNNNNNNGG 2 cut(s) 982, 1132
BseMI GCAATG 1 cut(s) 495
BseMII CTCAG 6 cut(s) 81, 126, 258, 1090, 1122, 1173
BseNI ACTGG 3 cut(s) 647, 833, 887
BseXI GCAGC 3 cut(s) 790, 812, 1013
Bsh1236I CGCG 1 cut(s) 62
BshFI GGCC 3 cut(s) 162, 283, 1180
BshNI GGYRCC 1 cut(s) 513
BsiHKAI GWGCWC 2 cut(s) 48, 1188
BsiSI CCGG 5 cut(s) 37, 56, 459, 532, 1062
BslI CCNNNNNNNGG 2 cut(s) 982, 1132
BsmAI GTCTC 5 cut(s) 65, 995, 1059, 1077, 1094
BsmBI CGTCTC 1 cut(s) 1094
BsnI GGCC 3 cut(s) 162, 283, 1180
Bsp119I TTCGAA 2 cut(s) 16, 600
Bsp1286I GDGCHC 2 cut(s) 48, 1188
Bsp13I TCCGGA 1 cut(s) 1061
Bsp1407I TGTACA 1 cut(s) 867
Bsp143I GATC 7 cut(s) 10, 407, 643, 760, 838, 987, 1116
BspACI CCGC 3 cut(s) 8, 356, 610
BspANI GGCC 3 cut(s) 162, 283, 1180
BspCNI CTCAG 6 cut(s) 82, 127, 259, 1089, 1123, 1174
BspEI TCCGGA 1 cut(s) 1061
BspFNI CGCG 1 cut(s) 62
BspLI GGNNCC 3 cut(s) 515, 645, 974
BspMAI CTGCAG 1 cut(s) 808
BspPI GGATC 4 cut(s) 18, 638, 651, 833
BspT104I TTCGAA 2 cut(s) 16, 600
BspT107I GGYRCC 1 cut(s) 513
BsrDI GCAATG 1 cut(s) 495
BsrGI TGTACA 1 cut(s) 867
BsrI ACTGG 3 cut(s) 647, 833, 887
BssECI CCNNGG 1 cut(s) 163
BssMI GATC 7 cut(s) 10, 407, 643, 760, 838, 987, 1116
BssT1I CCWWGG 1 cut(s) 163
Bst2UI CCWGG 2 cut(s) 285, 377
Bst4CI ACNGT 5 cut(s) 76, 82, 691, 895, 1006
BstAPI GCANNNNNTGC 1 cut(s) 422
BstAUI TGTACA 1 cut(s) 867
BstBI TTCGAA 2 cut(s) 16, 600
BstDEI CTNAG 8 cut(s) 90, 135, 267, 445, 1076, 1131, 1182, 1197
BstENI CCTNNNNNAGG 1 cut(s) 1130
BstF5I GGATG 5 cut(s) 121, 307, 339, 540, 669
BstFNI CGCG 1 cut(s) 62
BstHHI GCGC 1 cut(s) 208
BstKTI GATC 7 cut(s) 13, 410, 646, 763, 841, 990, 1119
BstMAI GTCTC 5 cut(s) 65, 995, 1059, 1077, 1094
BstMBI GATC 7 cut(s) 10, 407, 643, 760, 838, 987, 1116
BstMWI GCNNNNNNNGC 5 cut(s) 52, 229, 422, 812, 1202
BstNI CCWGG 2 cut(s) 285, 377
BstNSI RCATGY 1 cut(s) 1072
BstSCI CCNGG 3 cut(s) 283, 375, 530
BstSFI CTRYAG 1 cut(s) 804
BstUI CGCG 1 cut(s) 62
BstV1I GCAGC 3 cut(s) 790, 812, 1013
BstV2I GAAGAC 1 cut(s) 932
BstX2I RGATCY 3 cut(s) 10, 407, 643
BstYI RGATCY 3 cut(s) 10, 407, 643
Bsu36I CCTNAGG 1 cut(s) 1131
BsuRI GGCC 3 cut(s) 162, 283, 1180
BtsCI GGATG 5 cut(s) 121, 307, 339, 540, 669
BtsIMutI CAGTG 5 cut(s) 87, 654, 900, 914, 1011
CfoI GCGC 1 cut(s) 208
Cfr13I GGNCC 1 cut(s) 39
CpoI CGGWCCG 1 cut(s) 39
CseI GACGC 2 cut(s) 68, 1111
Csp6I GTAC 3 cut(s) 514, 868, 943
CspI CGGWCCG 1 cut(s) 39
CviAII CATG 6 cut(s) 112, 145, 485, 977, 1069, 1154
CviQI GTAC 3 cut(s) 514, 868, 943
DdeI CTNAG 8 cut(s) 90, 135, 267, 445, 1076, 1131, 1182, 1197
DpnI GATC 7 cut(s) 12, 409, 645, 762, 840, 989, 1118
DpnII GATC 7 cut(s) 10, 407, 643, 760, 838, 987, 1116
DriI GACNNNNNGTC 1 cut(s) 454
Eam1105I GACNNNNNGTC 1 cut(s) 454
EciI GGCGGA 1 cut(s) 23
Ecl136II GAGCTC 1 cut(s) 46
Eco130I CCWWGG 1 cut(s) 163
Eco147I AGGCCT 1 cut(s) 1180
Eco24I GRGCYC 1 cut(s) 48
Eco47I GGWCC 1 cut(s) 39
Eco53kI GAGCTC 1 cut(s) 46
Eco57I CTGAAG 2 cut(s) 378, 1091
Eco81I CCTNAGG 1 cut(s) 1131
EcoICRI GAGCTC 1 cut(s) 46
EcoNI CCTNNNNNAGG 1 cut(s) 1130
EcoRII CCWGG 2 cut(s) 283, 375
EcoT14I CCWWGG 1 cut(s) 163
EcoT38I GRGCYC 1 cut(s) 48
ErhI CCWWGG 1 cut(s) 163
Esp3I CGTCTC 1 cut(s) 1094
FaeI CATG 6 cut(s) 115, 148, 488, 980, 1072, 1157
FatI CATG 6 cut(s) 111, 144, 484, 976, 1068, 1153
FblI GTMKAC 1 cut(s) 1158
Fnu4HI GCNGC 5 cut(s) 357, 611, 801, 804, 1027
FokI GGATG 5 cut(s) 128, 314, 346, 547, 676
FriOI GRGCYC 1 cut(s) 48
Fsp4HI GCNGC 5 cut(s) 357, 611, 801, 804, 1027
FspI TGCGCA 1 cut(s) 207
GlaI GCGC 1 cut(s) 207
GluI GCNGC 5 cut(s) 357, 611, 801, 804, 1027
HaeIII GGCC 3 cut(s) 162, 283, 1180
HapII CCGG 5 cut(s) 37, 56, 459, 532, 1062
HgaI GACGC 2 cut(s) 68, 1111
HhaI GCGC 1 cut(s) 208
Hin1II CATG 6 cut(s) 115, 148, 488, 980, 1072, 1157
Hin6I GCGC 1 cut(s) 206
HinP1I GCGC 1 cut(s) 206
HincII GTYRAC 1 cut(s) 1159
HindII GTYRAC 1 cut(s) 1159
HindIII AAGCTT 1 cut(s) 230
HinfI GANTC 9 cut(s) 23, 120, 149, 243, 257, 437, 602, 628, 726
HpaII CCGG 5 cut(s) 37, 56, 459, 532, 1062
HphI GGTGA 1 cut(s) 319
Hpy166II GTNNAC 6 cut(s) 178, 310, 477, 870, 1048, 1159
Hpy188I TCNGA 4 cut(s) 31, 268, 966, 1097
Hpy188III TCNNGA 6 cut(s) 730, 758, 1062, 1109, 1130, 1212
Hpy8I GTNNAC 6 cut(s) 178, 310, 477, 870, 1048, 1159
Hpy99I CGWCG 1 cut(s) 67
HpyAV CCTTC 2 cut(s) 137, 1136
HpyCH4III ACNGT 5 cut(s) 76, 82, 691, 895, 1006
HpyCH4V TGCA 8 cut(s) 320, 488, 538, 563, 686, 800, 806, 1029
HpyF10VI GCNNNNNNNGC 5 cut(s) 52, 229, 422, 812, 1202
HpyF3I CTNAG 8 cut(s) 90, 135, 267, 445, 1076, 1131, 1182, 1197
Hsp92II CATG 6 cut(s) 115, 148, 488, 980, 1072, 1157
HspAI GCGC 1 cut(s) 206
Kpn2I TCCGGA 1 cut(s) 1061
KpnI GGTACC 1 cut(s) 517
Kzo9I GATC 7 cut(s) 10, 407, 643, 760, 838, 987, 1116
LmnI GCTCC 1 cut(s) 848
Lsp1109I GCAGC 3 cut(s) 790, 812, 1013
LweI GCATC 3 cut(s) 104, 525, 572
MaeIII GTNAC 2 cut(s) 76, 912
MalI GATC 7 cut(s) 12, 409, 645, 762, 840, 989, 1118
MboI GATC 7 cut(s) 10, 407, 643, 760, 838, 987, 1116
MboII GAAGA 3 cut(s) 5, 18, 937
MflI RGATCY 3 cut(s) 10, 407, 643
MhlI GDGCHC 2 cut(s) 48, 1188
MluCI AATT 6 cut(s) 201, 420, 549, 738, 789, 953
MlyI GAGTC 2 cut(s) 251, 622
MmeI TCCRAC 1 cut(s) 826
MnlI CCTC 8 cut(s) 85, 96, 183, 213, 298, 461, 1036, 1126
MroI TCCGGA 1 cut(s) 1061
MroXI GAANNNNTTC 2 cut(s) 741, 1125
MseI TTAA 2 cut(s) 185, 293
MslI CAYNNNNRTG 1 cut(s) 1191
MspA1I CMGCKG 2 cut(s) 134, 803
MspI CCGG 5 cut(s) 37, 56, 459, 532, 1062
MspR9I CCNGG 3 cut(s) 285, 377, 532
MvaI CCWGG 2 cut(s) 285, 377
MvnI CGCG 1 cut(s) 62
MwoI GCNNNNNNNGC 5 cut(s) 52, 229, 422, 812, 1202
NciI CCSGG 1 cut(s) 532
NdeII GATC 7 cut(s) 10, 407, 643, 760, 838, 987, 1116
NlaIII CATG 6 cut(s) 115, 148, 488, 980, 1072, 1157
NlaIV GGNNCC 3 cut(s) 515, 645, 974
NmuCI GTSAC 2 cut(s) 76, 912
NsbI TGCGCA 1 cut(s) 207
NspI RCATGY 1 cut(s) 1072
NspV TTCGAA 2 cut(s) 16, 600
PceI AGGCCT 1 cut(s) 1180
PciI ACATGT 1 cut(s) 1068
PcsI WCGNNNNNNNCGW 2 cut(s) 59, 1155
PdmI GAANNNNTTC 2 cut(s) 741, 1125
PfeI GAWTC 7 cut(s) 23, 120, 149, 243, 437, 602, 726
PkrI GCNGC 5 cut(s) 358, 612, 802, 805, 1028
PleI GAGTC 2 cut(s) 251, 622
PpsI GAGTC 2 cut(s) 251, 622
PscI ACATGT 1 cut(s) 1068
Psp124BI GAGCTC 1 cut(s) 48
Psp6I CCWGG 2 cut(s) 283, 375
PspGI CCWGG 2 cut(s) 283, 375
PspN4I GGNNCC 3 cut(s) 515, 645, 974
PspPI GGNCC 1 cut(s) 39
PstI CTGCAG 1 cut(s) 808
PsuI RGATCY 3 cut(s) 10, 407, 643
PvuII CAGCTG 2 cut(s) 134, 803
RsaI GTAC 3 cut(s) 515, 869, 944
RsaNI GTAC 3 cut(s) 514, 868, 943
RseI CAYNNNNRTG 1 cut(s) 1191
Rsr2I CGGWCCG 1 cut(s) 39
RsrII CGGWCCG 1 cut(s) 39
SacI GAGCTC 1 cut(s) 48
SalI GTCGAC 1 cut(s) 1157
SaqAI TTAA 2 cut(s) 185, 293
SatI GCNGC 5 cut(s) 357, 611, 801, 804, 1027
Sau3AI GATC 7 cut(s) 10, 407, 643, 760, 838, 987, 1116
Sau96I GGNCC 1 cut(s) 39
ScaI AGTACT 1 cut(s) 944
SchI GAGTC 2 cut(s) 251, 622
ScrFI CCNGG 3 cut(s) 285, 377, 532
SduI GDGCHC 2 cut(s) 48, 1188
SfaNI GCATC 3 cut(s) 104, 525, 572
SfcI CTRYAG 1 cut(s) 804
SfuI TTCGAA 2 cut(s) 16, 600
SinI GGWCC 1 cut(s) 39
SmiMI CAYNNNNRTG 1 cut(s) 1191
SmlI CTYRAG 1 cut(s) 756
SmoI CTYRAG 1 cut(s) 756
Sse9I AATT 6 cut(s) 201, 420, 549, 738, 789, 953
SseBI AGGCCT 1 cut(s) 1180
SsiI CCGC 3 cut(s) 8, 356, 610
SspI AATATT 2 cut(s) 212, 594
SstI GAGCTC 1 cut(s) 48
StuI AGGCCT 1 cut(s) 1180
StyD4I CCNGG 3 cut(s) 283, 375, 530
StyI CCWWGG 1 cut(s) 163
TaaI ACNGT 5 cut(s) 76, 82, 691, 895, 1006
TaqI TCGA 5 cut(s) 16, 600, 745, 1119, 1158
TaqII GACCGA 1 cut(s) 56
TasI AATT 6 cut(s) 201, 420, 549, 738, 789, 953
TatI WGTACW 2 cut(s) 867, 942
TauI GCSGC 2 cut(s) 359, 613
TfiI GAWTC 7 cut(s) 23, 120, 149, 243, 437, 602, 726
Tru1I TTAA 2 cut(s) 185, 293
Tru9I TTAA 2 cut(s) 185, 293
TscAI CASTG 5 cut(s) 87, 654, 900, 921, 1011
TseFI GTSAC 2 cut(s) 76, 912
TseI GCWGC 3 cut(s) 800, 803, 1026
Tsp45I GTSAC 2 cut(s) 76, 912
TspDTI ATGAA 3 cut(s) 342, 454, 1142
TspGWI ACGGA 1 cut(s) 801
TspRI CASTG 5 cut(s) 87, 654, 900, 921, 1011
VpaK11BI GGWCC 1 cut(s) 39
XagI CCTNNNNNAGG 1 cut(s) 1130
XceI RCATGY 1 cut(s) 1072
XmiI GTMKAC 1 cut(s) 1158
XmnI GAANNNNTTC 2 cut(s) 741, 1125
ZrmI AGTACT 1 cut(s) 944
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.