Rh5DG170300

Metal tolerance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0001629
Physical Location & Seq
Reverse (-)
18269294 .. 18272272
2979 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG170300.1

Sequence Viewer

Length: 897 bp
ATGACACAGCTTGAAAGGAGTGAAAGGATAGCAATATATGCATCCAATGCGGCTAATTTGGTGCTCTTCCTAGCAAAAGTGTATGCTTCTATTCAGAGCAGATCATTGGCAGTAATAGCTTCAACTTTGGACTCCCTCTTAGACCTCTTGTCAGGGTTCATCCTTTGGTTTACAGCTTCTGCAATGAGAAAACCAAATCAGTATCACTACCCGATTGGCAAGACTCGGATGCAACCTGTGGGAATTGTTGTTTTTGCATCAGTAATGGCAACTCTTGGATTGCAAATTTTGTTTGAATCAGGCCGACAACTTGTCATGAAGACTCAACCTGATAAGGACCCAGAGAAAGAGAAATGGATGATAGGGATAATGGTCTCTGTCACAATAGTCAAGATTGTTCTAACGGTATACTGTCGAAGATTCAAAAACGAAATTGTTCGAGCATATGCTCAAGATCATCTATTTGATGTCATCACTAATGCAATTGGTCTTGCATCAGCAGTGTTAGCAGTCAGGTTTTACTGGTGGATCGATCCAATTGGAGCTATCATCATTGCTTTGTATACAATGGCGAATTGGGCAAAAACAGTGATGGACAATGTGTGGTCGCTAATTGGGAAGACAGCACCAGCAGAGTACTTGGCCAAGTTAACATATCTAATTTGGAACCATGACAAGGAGATCAAGCACATTGAGACAGTGAGAGCATACACATTTGGTGTTAACTATTTTGTAGAGGTTCATGTAGTCTTACCTGGGGACATGTCTCTCAGTGATGCACATAATATCGGAGAGGCACTCCAAGAAAAGCTTGAGAATCTTCCTGAGGTTGAACGAGCTTTTGTTCATGTTGATGTTGACATTACTCACAAGCCAGAGCACAAGCCAAAGGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cation_efflux ZT_dimer

Protein Analysis

298

Amino Acids

33.68

Weight (kDa)

7.83

Isoelectric Point (pI)

36.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_efflux PF01545 14 - 204 5.9e-33 Cation efflux transmembrane domain
ZT_dimer PF16916 216 - 285 1.8e-10 Cation efflux protein, cytoplasmic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 408, 563
AciI CCGC 1 cut(s) 50
AclWI GGATC 2 cut(s) 527, 536
AcoI YGGCCR 1 cut(s) 642
AcsI RAATTY 1 cut(s) 285
AfaI GTAC 1 cut(s) 638
AfiI CCNNNNNNNGG 1 cut(s) 676
AflIII ACRYGT 1 cut(s) 762
AgsI TTSAA 5 cut(s) 14, 123, 296, 424, 833
AhdI GACNNNNNGTC 2 cut(s) 148, 311
AjnI CCWGG 1 cut(s) 754
AluBI AGCT 6 cut(s) 10, 119, 176, 545, 811, 839
AluI AGCT 6 cut(s) 10, 119, 176, 545, 811, 839
Alw21I GWGCWC 2 cut(s) 66, 882
Alw26I GTCTC 3 cut(s) 379, 689, 771
AlwI GGATC 2 cut(s) 527, 536
AlwNI CAGNNNCTG 1 cut(s) 179
AoxI GGCC 2 cut(s) 301, 642
ApoI RAATTY 1 cut(s) 285
Asp700I GAANNNNTTC 1 cut(s) 435
AspS9I GGNCC 1 cut(s) 337
AvaII GGWCC 1 cut(s) 337
AxyI CCTNAGG 1 cut(s) 825
BalI TGGCCA 1 cut(s) 644
BbsI GAAGAC 2 cut(s) 326, 626
Bbv12I GWGCWC 2 cut(s) 66, 882
BccI CCATC 1 cut(s) 586
BciT130I CCWGG 1 cut(s) 756
BcoDI GTCTC 3 cut(s) 379, 689, 771
BfaI CTAG 1 cut(s) 71
BisI GCNGC 1 cut(s) 51
BlsI GCNGC 1 cut(s) 52
BmcAI AGTACT 1 cut(s) 638
Bme1390I CCNGG 1 cut(s) 756
Bme18I GGWCC 1 cut(s) 337
BmeRI GACNNNNNGTC 2 cut(s) 148, 311
BmgT120I GGNCC 1 cut(s) 337
BmiI GGNNCC 2 cut(s) 339, 668
BmrFI CCNGG 1 cut(s) 756
BmsI GCATC 5 cut(s) 50, 219, 266, 503, 766
BpiI GAAGAC 2 cut(s) 326, 626
BplI GAGNNNNNCTC 2 cut(s) 783, 815
BpuEI CTTGAG 2 cut(s) 435, 833
Bsa29I ATCGAT 1 cut(s) 531
BsaI GGTCTC 1 cut(s) 379
BsaJI CCNNGG 1 cut(s) 755
Bsc4I CCNNNNNNNGG 1 cut(s) 676
Bse1I ACTGG 1 cut(s) 527
Bse21I CCTNAGG 1 cut(s) 825
Bse3DI GCAATG 2 cut(s) 189, 552
BseBI CCWGG 1 cut(s) 756
BseCI ATCGAT 1 cut(s) 531
BseDI CCNNGG 1 cut(s) 755
BseGI GGATG 4 cut(s) 41, 159, 234, 363
BseLI CCNNNNNNNGG 1 cut(s) 676
BseMI GCAATG 2 cut(s) 189, 552
BseMII CTCAG 2 cut(s) 784, 816
BseNI ACTGG 1 cut(s) 527
BshFI GGCC 2 cut(s) 303, 644
BshVI ATCGAT 1 cut(s) 531
BsiHKAI GWGCWC 2 cut(s) 66, 882
BslFI GGGAC 1 cut(s) 773
BslI CCNNNNNNNGG 1 cut(s) 676
BsmAI GTCTC 3 cut(s) 379, 689, 771
BsmFI GGGAC 1 cut(s) 773
BsnI GGCC 2 cut(s) 303, 644
Bso31I GGTCTC 1 cut(s) 379
Bsp1286I GDGCHC 2 cut(s) 66, 882
Bsp143I GATC 5 cut(s) 101, 454, 528, 532, 681
BspACI CCGC 1 cut(s) 50
BspANI GGCC 2 cut(s) 303, 644
BspCNI CTCAG 2 cut(s) 783, 817
BspDI ATCGAT 1 cut(s) 531
BspHI TCATGA 1 cut(s) 315
BspLI GGNNCC 2 cut(s) 339, 668
BspPI GGATC 2 cut(s) 527, 536
BspQI GCTCTTC 1 cut(s) 71
BspTNI GGTCTC 1 cut(s) 379
BsrDI GCAATG 2 cut(s) 189, 552
BsrI ACTGG 1 cut(s) 527
BssECI CCNNGG 1 cut(s) 755
BssMI GATC 5 cut(s) 101, 454, 528, 532, 681
BssNAI GTATAC 2 cut(s) 409, 564
Bst1107I GTATAC 2 cut(s) 409, 564
Bst2UI CCWGG 1 cut(s) 756
Bst4CI ACNGT 4 cut(s) 406, 413, 589, 700
Bst6I CTCTTC 1 cut(s) 71
BstAPI GCANNNNNTGC 2 cut(s) 38, 47
BstDEI CTNAG 3 cut(s) 139, 770, 825
BstF5I GGATG 4 cut(s) 41, 159, 234, 363
BstKTI GATC 5 cut(s) 104, 457, 531, 535, 684
BstMAI GTCTC 3 cut(s) 379, 689, 771
BstMBI GATC 5 cut(s) 101, 454, 528, 532, 681
BstMWI GCNNNNNNNGC 5 cut(s) 38, 47, 116, 506, 578
BstNI CCWGG 1 cut(s) 756
BstNSI RCATGY 1 cut(s) 766
BstSCI CCNGG 1 cut(s) 754
BstV2I GAAGAC 2 cut(s) 326, 626
BstZ17I GTATAC 2 cut(s) 409, 564
Bsu15I ATCGAT 1 cut(s) 531
Bsu36I CCTNAGG 1 cut(s) 825
BsuRI GGCC 2 cut(s) 303, 644
BsuTUI ATCGAT 1 cut(s) 531
BtsCI GGATG 4 cut(s) 41, 159, 234, 363
BtsI GCAGTG 1 cut(s) 507
BtsIMutI CAGTG 4 cut(s) 507, 594, 705, 778
CaiI CAGNNNCTG 1 cut(s) 179
CciI TCATGA 1 cut(s) 315
Cfr13I GGNCC 1 cut(s) 337
ClaI ATCGAT 1 cut(s) 531
Csp6I GTAC 1 cut(s) 637
CviAII CATG 5 cut(s) 316, 671, 743, 763, 848
CviQI GTAC 1 cut(s) 637
DdeI CTNAG 3 cut(s) 139, 770, 825
DpnI GATC 5 cut(s) 103, 456, 530, 534, 683
DpnII GATC 5 cut(s) 101, 454, 528, 532, 681
DriI GACNNNNNGTC 2 cut(s) 148, 311
EaeI YGGCCR 1 cut(s) 642
Eam1104I CTCTTC 1 cut(s) 71
Eam1105I GACNNNNNGTC 2 cut(s) 148, 311
EarI CTCTTC 1 cut(s) 71
Eco31I GGTCTC 1 cut(s) 379
Eco47I GGWCC 1 cut(s) 337
Eco81I CCTNAGG 1 cut(s) 825
EcoO109I RGGNCCY 1 cut(s) 337
EcoRII CCWGG 1 cut(s) 754
EcoT22I ATGCAT 1 cut(s) 43
FaeI CATG 5 cut(s) 319, 674, 746, 766, 851
FalI AAGNNNNNCTT 2 cut(s) 795, 827
FaqI GGGAC 1 cut(s) 773
FatI CATG 5 cut(s) 315, 670, 742, 762, 847
FauNDI CATATG 1 cut(s) 445
FblI GTMKAC 2 cut(s) 408, 563
Fnu4HI GCNGC 1 cut(s) 51
FokI GGATG 4 cut(s) 28, 146, 241, 370
Fsp4HI GCNGC 1 cut(s) 51
FspBI CTAG 1 cut(s) 71
GluI GCNGC 1 cut(s) 51
HaeIII GGCC 2 cut(s) 303, 644
Hin1II CATG 5 cut(s) 319, 674, 746, 766, 851
HincII GTYRAC 3 cut(s) 651, 724, 859
HindII GTYRAC 3 cut(s) 651, 724, 859
HindIII AAGCTT 1 cut(s) 809
HinfI GANTC 6 cut(s) 131, 223, 296, 322, 420, 817
HpaI GTTAAC 2 cut(s) 651, 724
Hpy166II GTNNAC 6 cut(s) 171, 409, 564, 651, 724, 859
Hpy188I TCNGA 3 cut(s) 96, 228, 791
Hpy188III TCNNGA 4 cut(s) 316, 391, 452, 824
Hpy8I GTNNAC 6 cut(s) 171, 409, 564, 651, 724, 859
HpyCH4III ACNGT 4 cut(s) 406, 413, 589, 700
HpyCH4V TGCA 8 cut(s) 41, 182, 232, 257, 283, 482, 494, 779
HpyF10VI GCNNNNNNNGC 5 cut(s) 38, 47, 116, 506, 578
HpyF3I CTNAG 3 cut(s) 139, 770, 825
Hsp92II CATG 5 cut(s) 319, 674, 746, 766, 851
KspAI GTTAAC 2 cut(s) 651, 724
Kzo9I GATC 5 cut(s) 101, 454, 528, 532, 681
LguI GCTCTTC 1 cut(s) 71
LmnI GCTCC 1 cut(s) 542
LweI GCATC 5 cut(s) 50, 219, 266, 503, 766
MaeI CTAG 1 cut(s) 71
MaeIII GTNAC 1 cut(s) 379
MalI GATC 5 cut(s) 103, 456, 530, 534, 683
MboI GATC 5 cut(s) 101, 454, 528, 532, 681
MboII GAAGA 5 cut(s) 58, 331, 429, 631, 812
MfeI CAATTG 2 cut(s) 483, 537
MhlI GDGCHC 2 cut(s) 66, 882
MlsI TGGCCA 1 cut(s) 644
MluCI AATT 9 cut(s) 55, 243, 285, 432, 483, 537, 574, 612, 660
MluNI TGGCCA 1 cut(s) 644
MlyI GAGTC 3 cut(s) 125, 217, 316
MnlI CCTC 5 cut(s) 146, 155, 730, 787, 820
Mox20I TGGCCA 1 cut(s) 644
Mph1103I ATGCAT 1 cut(s) 43
MroXI GAANNNNTTC 1 cut(s) 435
MscI TGGCCA 1 cut(s) 644
MseI TTAA 2 cut(s) 650, 723
MslI CAYNNNNRTG 1 cut(s) 852
Msp20I TGGCCA 1 cut(s) 644
MspR9I CCNGG 1 cut(s) 756
MunI CAATTG 2 cut(s) 483, 537
MvaI CCWGG 1 cut(s) 756
MwoI GCNNNNNNNGC 5 cut(s) 38, 47, 116, 506, 578
NdeI CATATG 1 cut(s) 445
NdeII GATC 5 cut(s) 101, 454, 528, 532, 681
NlaIII CATG 5 cut(s) 319, 674, 746, 766, 851
NlaIV GGNNCC 2 cut(s) 339, 668
NmuCI GTSAC 1 cut(s) 379
NsiI ATGCAT 1 cut(s) 43
NspI RCATGY 1 cut(s) 766
PagI TCATGA 1 cut(s) 315
PciI ACATGT 1 cut(s) 762
PciSI GCTCTTC 1 cut(s) 71
PdmI GAANNNNTTC 1 cut(s) 435
PfeI GAWTC 3 cut(s) 296, 420, 817
PkrI GCNGC 1 cut(s) 52
PleI GAGTC 3 cut(s) 125, 217, 316
PpsI GAGTC 3 cut(s) 125, 217, 316
PpuMI RGGWCCY 1 cut(s) 337
PscI ACATGT 1 cut(s) 762
Psp5II RGGWCCY 1 cut(s) 337
Psp6I CCWGG 1 cut(s) 754
PspGI CCWGG 1 cut(s) 754
PspN4I GGNNCC 2 cut(s) 339, 668
PspPI GGNCC 1 cut(s) 337
PspPPI RGGWCCY 1 cut(s) 337
PstNI CAGNNNCTG 1 cut(s) 179
RsaI GTAC 1 cut(s) 638
RsaNI GTAC 1 cut(s) 637
RseI CAYNNNNRTG 1 cut(s) 852
SapI GCTCTTC 1 cut(s) 71
SaqAI TTAA 2 cut(s) 650, 723
SatI GCNGC 1 cut(s) 51
Sau3AI GATC 5 cut(s) 101, 454, 528, 532, 681
Sau96I GGNCC 1 cut(s) 337
ScaI AGTACT 1 cut(s) 638
SchI GAGTC 3 cut(s) 125, 217, 316
ScrFI CCNGG 1 cut(s) 756
SduI GDGCHC 2 cut(s) 66, 882
SfaNI GCATC 5 cut(s) 50, 219, 266, 503, 766
SinI GGWCC 1 cut(s) 337
SmiMI CAYNNNNRTG 1 cut(s) 852
SmlI CTYRAG 2 cut(s) 450, 812
SmoI CTYRAG 2 cut(s) 450, 812
Sse9I AATT 9 cut(s) 55, 243, 285, 432, 483, 537, 574, 612, 660
SsiI CCGC 1 cut(s) 50
SspMI CTAG 1 cut(s) 71
StyD4I CCNGG 1 cut(s) 754
TaaI ACNGT 4 cut(s) 406, 413, 589, 700
TaqI TCGA 3 cut(s) 415, 439, 531
TasI AATT 9 cut(s) 55, 243, 285, 432, 483, 537, 574, 612, 660
TatI WGTACW 1 cut(s) 636
TauI GCSGC 1 cut(s) 53
TfiI GAWTC 3 cut(s) 296, 420, 817
Tru1I TTAA 2 cut(s) 650, 723
Tru9I TTAA 2 cut(s) 650, 723
TscAI CASTG 4 cut(s) 507, 594, 705, 778
TseFI GTSAC 1 cut(s) 379
Tsp45I GTSAC 1 cut(s) 379
TspDTI ATGAA 4 cut(s) 148, 332, 731, 836
TspRI CASTG 4 cut(s) 507, 594, 705, 778
VpaK11BI GGWCC 1 cut(s) 337
XapI RAATTY 1 cut(s) 285
XceI RCATGY 1 cut(s) 766
XmiI GTMKAC 2 cut(s) 408, 563
XmnI GAANNNNTTC 1 cut(s) 435
XspI CTAG 1 cut(s) 71
ZrmI AGTACT 1 cut(s) 638
Zsp2I ATGCAT 1 cut(s) 43
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.